A ndro V ision ® - m o r e t h a n C ASA

AndroVision® start screen

:::::::::::: Computerized semen analysis

AndroVision® is a highly efficient CASA* system for standardized,

interactive semen analysis. The basic system with PC and accessories
is complemented by optional software modules. AndroVision®
provides different options for viability assays of sperm functionality
besides classic automatic analysis of motility, concentration and
morphology.
*CASA= Computer Assisted Sperm Analysis
:::::::::::: Basic system
AndroVision® software
with PC and accessories REF. : 12500/0000
AndroVision® camera
High speed and high resolution REF. : 12500/4200
AndroVision® user interface - clear and intuitive
Product features ::::::::::::
• Real time analysis of live images and video files
• Very high sperm count per field analysis (> 1000)
• Highly efficient particle filter for accurate sperm differentiation
• Analysis profiles of many species
• Data base with analysis results, including AVI video files
• Data export to MS Excel and other programs
• Adjustable analysis settings and user interface
• Semen analysis report individually designed, includes analysis
image
• Languages: English, Spanish, Chinese, Portuguese, Russian, German

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:::::::::::: Software modules
Morphology and Morphometry:
Interactive system for analysis of sperm morphology and morphometry.
Identifies sperm of stained and unstained samples and measures length
and width of the sperm head, head shape and midpiece asymmetry of
each single sperm cell (acc. to Krüger). Results can be classified into a
large range of morphologic abnormalities. The classification according
to morphological criteria is a self-learning process.
Module Morphology & Morphometry REF. : 12500/1300

Viability:
Morphologie and Morphometry screen
Automated count of the percentage of membrane intact sperm, based
on a double stain fluorescence assay.
Module Viability* REF. : 12500/1400

Acrosome integrity:
Automated count of sperm with damaged acrosome, based on a
double stain fluorescence assay.
Module Acrosome integrity* REF. : 12500/1600

Dose calculation:
Calculating of the number of doses and volume of extender to add to
the ejaculate. Viability analysis based on Hoechst/PI stained sperm sample
Module Dose calculation REF. : 12500/1100

Quality control:
Microscope ::::::::::::
Analysis of samples post thaw and in holding. Link to native
ejaculate analysis Possibility to analyze samples during production: AndroVision® can be combined with a series of microscopes, preferably:
Samples of one ejaculate can be analyzed more than once and be
• Zeiss AxioLab and AxioScope
compared to native ejaculate values.
Module Quality control REF. : 12500/1200 • Olympus CX31/41 and BX series

Lab software link: Each microscope requires a negative phase contrast objective and a
heated stage. The software modules Viability and Acrosome integrity
Link of AndroVision® with lab software (e.g. IDA/IDEE).
require fluorescence equipment. Morphology analysis uses 40x or
Module Lab software link REF. : 12500/1910
100x phase contrast objective.
* requires flourescence microscope

:::::::::::: Fluorescence stains

SYBR14/Pl Membrane integrity (Viability)
for up to 100 assays REF. : 15407/0001

Hoechst 33342/Pl Membrane integrity (Viability)

for up to 100 assays REF. : 15407/0009

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Your benefits
Accuracy of sperm detection: A very accurate particle filter
distinguishes sperm from debris and egg yolk. Agglutinated cells
are excluded from evaluation. Concentration measurement has a
high repeatability and is very accurate.
Precise motility assessment: Progressive motility is assessed as
a sub-class of motility. Sperm cells moving in circles are correctly
identified. AndroVision® provides the standard CASA motility
parameters and WHO classification. Free definable classification
levels are available.
Quick analysis: AndroVision® allows for an accurate motility and
concentration assessment at production line speed for the semen
production lab.
Precision control: The combination of AndroVision® with an
optimal high end microscope makes sure that the operator stays in
control.
Flexibility: AndroVision® can analyze recorded videos as well
as live sperm samples. Analyzing recorded videos provides great
flexibility of semen assessment, both in time and location. Unlike
other similar products this system can be used with a range of
different counting chambers, providing the operator with flexibility
in his choice of product.
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AndroVision® analysis report
Morphology and Morphometry analysis: AndroVision® fea-
tures full analysis of abnormalities with an interactive tool. The user
is assisted with a thorough and complete analysis of morphological
abnormalities of sperm. It identifies the sperm of stained and un-
stained samples and takes measurements of each individual cell: The
Morphometry (acc. to Krüger) determines length and width of sperm
head, head shape and midpiece asymmetry of each single sperm cell
analysed. Sperm cells are classified into a large range of morphologic
abnormalities. The classification is a self learning process or can be set
to an automatic differentiation between intact and abnormal heads.
The analysis is done with a 40x or 100x phase contrast objective.
Custom made reports: AndroVision® can generate reports of all
analysis results as print-outs or MS Excel files. Production units and
analysis service labs can provide clients with a full quality report on
the semen doses. Reports can easily be edited by the user.
Custom made access level settings: AndroVision® can be custo-
mized for several operator levels such as user, manager or administrator.
Consistency: The objective AndroVision® evaluation criteria
guarantee the application of identical standards under all circum-
stances. This makes it possible to compare data of technicians, labs
and production days. Clearly defined threshold values allow detec-
tion of poor quality semen without a doubt and prevent processing,
freezing or shipping of sub standard product.
 AndroVision® functions at a glance

Functions
Concentration √
Motility: total, progressive, local, circular √
WHO motility classification √
Extended motility (7 additional classifications) √
Animal and analysis data base √
Analysis reports √
Advanced reports √
Parameter modification √
Export of analysis data √
Export of video files √
Compatible with many different microscopes √
Compatible with many different counting chambers √

:::::::::::: Motility analysis parameters:

• Total, progressive, local, circular Curvilinear VCL
path
• WHO classes: fast, slow, local, immotile
• VCL (Velocity Curve Line VAP
= distance and speed over the whole path) ALH
• VSL (Velocity Straight Line= speed direct path)
Average
path
• VAP (Velocity Average Path = average speed)
• ALH amplitude of lateral head displacement
VSL
• LIN linearity
Straight line path
• BCF (Beat-Cross Frequency
= average frequency of the travelled path over average
Subject to modifications, errors excepted. Illustrations similar. REF. : 30000/10195 | 07/2014

distance)

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