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Restriction Analysis Lecture Bioinformatics Uaar
Restriction Analysis Lecture Bioinformatics Uaar
Restriction Analysis Lecture Bioinformatics Uaar
Lecture Bioinformatics
UAAR
DNA and its bonds
• DNA molecules are macromolecules that hold
the genetic information of living organisms.
http://arbl.cvmbs.colostate.edu/
Different enzymes…different
sites…different cuts
http://www.phschool.com/science/ http://www.phschool.com/science/ http://www.phschool.com/science/
Incubate
Temperature and time depend on enzyme to be used.
http://www.kochi-u.ac.jp/~tatataa/tech2/gene/ligation.jpg
http://www.kochi-u.ac.jp/~tatataa/tech2/gene/ligation.jpg
Non-Examples
Restriction enzyme digestion is
NOT used for:
• Cutting RNA
• Sequencing DNA
Resources
http://www.phschool.com/science/biology_place/biocoach/red/intro.html
http://www.vivo.colostate.edu/hbooks/genetics/biotech/enzymes/cuteffects.html
http://web.indstate.edu/thcme/mwking/molecularmedicine.html#restriction
http://www.fermentas.com
http://www.chemsoc.org/timeline/graphic/1984_dnaf.jpg
END
Quick Quiz 1
A restriction enzyme cuts:
A. Single-stranded DNA
B. RNA
C. Proteins
D. Double-stranded DNA
Quick Quiz 2
A restriction enzyme works by identifying a specific DNA sequence
and cleaving:
• Restriction endonucleases are a special class of Endonuclease from bacteria to cut DNA.
EcoRI & Hind III. These are enzymes that digest DNA by recognizing specific short
sequences of bases called palindromic.