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Parabens are a group of chemicals that are derived from benzoic acid. Because of Based on the results obtained, methylparaben strongly inhibited the growth of all fungi
anti-bacterial property, parabens are classified in the group of preservatives. Inexpensive tested by zone of inhibition ranged from 25 to 35 mm after 48 h. This compound induced
price and excellent effect in preventing decomposition providing a widely use of a clear zone of fungal growth inhibition of 25 mm for A. niger, 30 mm for C. albicans
parabens to extend the life of food products and cosmetics from 50 years ago. In this and 35 mm for A. flavus (Figure 3). In this study, we demonstrated that methylparaben
study, growth inhibitory effect of methylparaben (Figure 1) was evaluated against showed the antifungal activity against human pathogenic fungi. According to the results,
Aspergillus niger , Aspergillus flavus and Candida albicans as important human as for low costs of production, chemical stability and low toxicity of methylparaben and
pathogenic fungi. rapid development of resistant strains to the old antifungal agents, these results suggest
that we may can use this compound as a novel antifungal agent with worldwide usage.
Methylparaben's derivatives with different structure and different alkyl chain length, may
have better antifungal activity than methylparaben; this issue and the operating
mechanism of these compounds need more study in future.
Zone of Inhibition (mm)
Figure 1 _ Methylparaben
The disk diffusion method was used to evaluate antifungal activity of methylparaben.200
µg Methylparaben dissolved in 800 µg ethanol (purity 98.0%) at room temperature and
inseminated on blank discs. Aspergillus niger, A. flavus and C. albicans were cultured on
Muller Hinton agar plates and blank discs containing methylparaben (400 µg/disc;
dissolved in ethanol) were placed on the surface of the culture plates. Cultures were Figure 3 _ Zone of inhibition diagram
maintained for 48 h at 30 °C (for Aspergillus species) and 37 °C (for C. albicans) and
the zone of inhibitions around the disks were measured and considered as an index of
antifungal activity (figure 2).
Conclusion
With respect to the strong antifungal activity, methylparaben may consider as a good
candidate for application in human and animal health practices as an antifungal as well as
a preservative for preventing food, drug and cosmetics fungal deterioration.
References