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Laboratory Manual Activity No. 2 Observing Bacteria
Laboratory Manual Activity No. 2 Observing Bacteria
Introduction
Bacteria are microorganisms that have a cell well, a cell membrane and a cytoplasm, nut not a
nucleus. Bacteria have three basic shapes: bacillus or rod-shaped, coccus or sphere shaped, and spirilla
or spiral-shaped.
Objective
1. Examine some prepared slides of bacteria and identify them according to their shape
2. Observe the structure of bacteria
Materials
Prepared slides of bacilli, cocci and spirilla
microscope
Procedure
1. Obtain three prepared slides of bacteria.
2. Place one slide on the stage of the microscope and observe the specimen using the low-power
objective lens.
3. Locate the bacteria. Identify its shape. Locate all three shapes of bacteria.
4. Switch to high-power objective lens. Focus on a few bacteria.
5. Draw what you see. Identify the parts of a bacteria: cell wall, cell membrane, and cytoplasm.
a. SPIRILLA b. COCCI
c. BACILLI
Discussion
It is much smaller in size than an animal cell, It is larger than a bacterial cell, e.g.
They are generally autotrophs and maybe They are always heterotrophs.
heterotrophs.
It has plasmids (circular DNA in the cytosol). It lacks the plasmids. It has well-
cell wall.
surface.
cytoplasm).
proteins.
Conclusion:
Questions
1. Describe ways by which bacteria are helpful and ways by which they are harmful.
Bacteria help many animals to digest food, they help trees grow, and they are important in the recycling
of nutrients in the environment. They are also used in biotechnology applications to produce everything
from food to energy to clean water. Bacteria can be very helpful to humans and other organisms.
Harmful bacteria are called pathogenic bacteria because they cause disease and illnesses like strep
throat, staph infections, cholera, tuberculosis, and food poisoning.
2. How do you protect yourself from bacteria that might infect you?
T
Bacterialcell:Bacterialcellis
aprokaryoticcell.
Animalcell:Animalcellisa
eukaryoticcell.
Size
BacterialCell:Bacterialcellsa
re0.2to2µminsize.
AnimalCell:Animalcellsare
largerinsizecomparedtob
acterialcellsand10to100µ
minsize.
Cellwall
BacterialCell:Thebacterialcel
lwallismadeupofmurein.
AnimalCell:Animalcellsdo
nothaveacellwall.Theplas
mamembraneistheouterbou
ndary.
Shape
BacterialCell:Bacterialcellsc
onsistofseveralshapeslikec
occui,bacillus,vibrio,spirilla.