Biodegradation of Polyethylene

using Endophytic fungi isolated

from Carabao Mango
(Mangifera Indica L.)
NALLIW, TRISHA JEANE | CADIENTE, JIREH ANGELA | ORDEN, KHEAN
STUDENT RESEARCHERS

APRIL 2021
BACKGROUND
OF THE STUDY
Carabao Mango
(Mangifera
indica L.)
a variety of particularly sweet
mango from the Philippines
Polyethylene
the most common plastic used
at present (abbreviated PE)
exposure to heat makes this a
threat to the human body
Endophytic Fungi
(Endophytes)
settle in robust plant tissues
without causing apparent
disease
the compounds produced by
endophytes have been shown
to have important medical
applications
Biodegradation
the process of breaking down of organic materials
through the use of microorganisms
specialized proteins called enzymes are involved
STATEMENT OF
THE PROBLEM
 PROBLEM

01 Does the endophytic fungi possess factors

that can break down PE plastic?

PROBLEM PROBLEM

This study aims to provide answers to

02 Does the endophytic fungi have the ability
to affect abiotic degradation through
physical, chemical enzymatic action?
the following questions:
PROBLEM

03 Can the endophytic fungi disintegrate PE

plastic considering the factors that it
possesses?
 STATEMENT OF
THE HYPOTHESIS
01 The endophytic fungi are not effective in
disintegrating PE plastic.

02 Endophytic fungi do not possess factors that NULL

HYPOTHESIS
disintegrate PE plastic.

03 The endophytic fungi do not have the ability to

affect abiotic degradation.
SIGNIFICANCE
OF THE STUDY
The results of this study could be beneficial to the
following:

01
This study could help people
exposed to polyethlene, which
pose a threat to their central
nervous system.
02
This investigation could help
future researchers generate
new ideas for research projects
related to biodegradation,
endophytic fungi and plastics.
03
This study could benefit the
studies about endophytic fungi
that could help companies who
are aiming to reduce plastic
wastes.
 SCOPE AND
LIMITATIONS
 Scope &
Limitations
This study will: This study will not:

focus on determining the effectiveness of use a specific fungus

the endophytic fungi in biodegrading the discuss other microorganisms and other
PE plastic types of plastics
aim to observe the biodegradability of PE
plastic through the use of endophytic
fungi
 RESEARCH
ENVIRONMENT
 Poblacion West, Lamut, Ifugao
Nueva Vizcaya State University Laboratory in
Bayombong, Nueva Vizcaya
MATERIALS AND
PROCEDURES
 1. Mango Sampling

2. Isolation of Endophytic Fungi

3. Identification of Endophytic Fungi

4. Sequencing and Phylogenetic Analysis

5. Initial Polyethylene Plastic Clearance Screen

6. Anaerobic Degradation of Polyethylene Plastic

EXPERIMENTAL
DESIGNS
 Experimental Design 1

Title: Biodegradation of Polyethylene plastic

using Endophytic fungi isolated from Carabao
mango
Independent Variable: Isolated fungi
Dependent Variable: Decay rate of
polyethylene pastic
Hypothesis: The fungi is effective in
disintegrating the polyethylene plastic
 Experimental Design 2

Title: Biodegradation of Polyethylene

plastic using isolated Fungi from Carabao
mango
Independent Variable: Isolated fungi
Dependent Variable: Decay rate of
polyethylene plastic
Hypothesis: The fungi has the ability to
disintegrate the polyethylene plastic
TREATMENT OF
DATA
The data will be analyzed using
descriptive statistics
RESEARCH
BUDGET
Budget plan
RESEARCH
TIMELINE
REFERENCES
 Mahesh S. Dashyal, C.G. Sangeetha, Vikram Appanna, G.K. Halesh and Devappa (2019).
Isolation and Morphological Characterization of Endophytic Fungi Isolated from Ten
Different Varieties of Mango. Int.J.Curr.Microbiol.App.Sci. 8(03): 717-726.

Petrini O. (1991). Fungal endophytes of tree leaves. In: Andrews

JH, Hirano SS, editors. Microbial ecology of leaves. p. 179–197.

Legaspi M. (2013). Bioremediation of plastic and rubber by endophytic fungi.

https://cfpub.epa.gov/ncer_abstracts/index.cfm/fuseaction/display.abstract/10317/re
port/0
Thank you
FOR LISTENING