ACKNOWLEDGEMENT

This study becomes a reality with the kind support and help of many individuals.

The researchers would like to extend their sincere thanks to all who played a big role in

this study.

Foremost, the researchers would like to offer this endeavor to God Almighty for

the wisdom bestowed upon, the strength, peace of mind, and good health in order to

finish the study.

The researchers would like to thank Sr. Consuelo Gacutan, the high school

directress and Mrs. Rosabel Rizalinda Aspiras, the high school principal for the approval

on conducting the research in school.

The researchers wish to grant appreciation to the selected grade 12 students of

batch 2019-2020 of Christ the King College—San Fernando City, La Union Inc., who

willingly take part and answered the survey questionnaire with honesty and coherence.

The researchers would like to honor Mr. Pablo Tadifa Jr. for imparting his

knowledge and professional expertise for the improvement of the study.

The researchers would also like to give credit and special thanks to Mr. Dexter

Shean G. Labitoria, Mrs. Maria Liza E. Hidalgo and to Ms. Khristine Jane C. Samera, our

research advisers, for being open for questions about the research, for patiently checking

papers and most especially for the giving guidance, enlightenment and recommendations

for this study all through. The gratitude and appreciations also go to the colleagues and

people who considered the work for the approval of the study and exemplary recognition.
 The researchers are beyond grateful to the moral support given by the family and

friends, together with people who associated with the study. This would not have been

possible without the support of people who take part all throughout the study 
 DEDICATION

The researchers wholeheartedly dedicate their dissertation work to the research

advisers and panelists who have been the source of motivation for this research and the

fruit of their patience that they have done.

This study is sincerely dedicated to their family whose untiring support and

assistance have made possible for the fruition of their efforts. To their friends, who

helped them develop their skills.

To all classmates and schoolmates who shared their words of advice to

accomplish this study.

To the institution, for this study is reflects its performance in providing good

quality of education and in educating youth with excellence.

Lastly, the researchers dedicate this research to Almighty God the creator of

knowledge, wisdom and understanding and upon showering much guidance, wisdom,

strength and power of mind. The one who gave them patience, and the gift of ability and

skills.
TABLE OF CONTENTS
I. Introduction.………………………………………………………………….1

A. Conceptual Framework……………………………………………………3

B. Statement of the Problem……………………………………………….....4

C. Hypothesis…………………………………………………………………4

D. Significance of the Study……………………………………………….....5

E. Scope and Delimitation……………………………………………………5

F. Definition of Terms………………………………………………………..6

II. Methodology……………………………………………………………….....7

A. Research Design…………………………………………………………...7

G. Tools for Data Analysis……………………………………………………

H. Instrumentation and Data Collection………………………………………

I. Locale and Population of the Study……………………………………….9

J. Ethical Consideration……………………………………………………...9

B. Results and Discussions………………………………………………….11

C. Summary, Conclusion and

Recommendations…………………………...23

K. Summary…………………………………………………………………23

L. Findings………………………………………………………………….23

M. Conclusion……………………………………………………………….24

N. Recommendations………………………………………………………..25

Bibliography
Appendices

Documentation
Title: Pulverized Grapefruit Seed and Allium sativum Extract as an Antimicrobial foot

cream

Authors:

Alvester, Lae Angela A.

Arnobit, Kharisza-Anne D.

Gacad, Raovi Mcwinowa O.

Galanta, Venus S.

Jusay, Jerick G.

Medidas, Charish Mae C.

Tiongco, Jericho D.

Advisers:

Mr. Dexter Shean G. Labitoria, Research in Daily Life 2 Teacher

Mrs. Maria Liza E. Hidalgo, Research Project Teacher

Ms. Khristine Jane C. Samera, Inquiries, Investigations and Immersion Teacher

Institution:

Christ the King College—San Fernando City, La Union, Inc.

Location:
Sevilla, City of San Fernando, La Union
Synopsis: The purpose of this study is to determine the efficacy of Pulverized Grapefruit

seed and Allium sativum Extract as an Antimicrobial foot cream.

Quantitative approach is used in the study that enables the researchers to produce

a study that is highly objective and deliver an accurate number of data and results. In

collecting relevant information, the pre-experimental research design was used in the

study. This design enables the researchers to test variables to gather data from it which

makes it more appropriate to thus study since it is aimed at gathering data test the

effectiveness of pulverized grapefruit seed and allium sativum extract as an antimicrobial

foot cram. Survey questionnaire were distributed to gather data evidences. There are ten

respondents from selected grade 12 students of Christ the King College—San Fernando

City, La Union Inc. Sample products are being observed by the respondents as their basis

for their responses.

The researchers found out that making antimicrobial foot cream out of pulverized

grapefruit seed and allium sativum extract is effective in terms of antimicrobial property,

treating athletes’ foot, and the duration. It has the potential in eliminating bad bacteria in

the different regions of feet and in treating fungi.

 CHAPTER I

INTRODUCTION

Background of the Study

Garlic (Allium sativum) has acquired a reputation in different traditions as a

prophylactic as well as therapeutic medicinal plant. Garlic has antibacterial, antifungal,

antiviral, and antiseptic properties from allicin. Allicin helps to kill the bacteria causing

skin infections. It also helps to reduce swelling and inflammation, and improve blood

circulation. These beneficial effects allow the skin to receive more nutrients. Grapefruit

(Citrus paradisi) has Vitamin C plays a vital role in the formation of collagen, the main

support system of the skin and conclude that vitamin C could help protect against sun

damage and aging. Citrus fruits such as oranges and grapefruits are particularly effective

at brightening and reviving the skin due to their acidic properties. Grapefruit in particular,

has recently become a key ingredient in skincare products and a staple in many skin

afflictions for those desiring a comforting complexion from this fruit. Included in many

cleansing products, also possesses antiseptic and antibacterial properties which work to

clean and clear congested skin for a satisfying outcome for the skin.

Nowadays, having skin infections is a common problem for the people, this is

caused by the exposure of the skin from dirty environment, and other things that can

result to skin reaction. Tinea pedis or athlete's foot is a superficial inflammatory infection

of the skin of the feet caused by dermatophyte fungi. Athlete's foot is characterized by

whitish macerated skin and itchy or asymptomatic erythema between the toes, usually in

the fourth and fifth spaces. Blisters and cracks in the skin between the toes may cause

1
pain and inflammation of the exposed raw tissue. A concomitant bacterial infection may

also be present and require antibiotic treatment. Athlete's foot is typically diagnosed by

visual inspection of cell culture and may be differentially diagnosed as bacterial

inflammatory and eczema. The most common complications of athlete's foot are

subsequent toenail infection or bacterial skin infection. These infections can be cured by

using grapefruit and garlic that contains organic and natural compounds that can

contribute in treating as such. Grapefruit and Allium sativum possess the capabilities of

being a good agent against infections and disease caused by bacteria. Grapefruit and

Allium sativum in antimicrobial treatment is essential in order to monitor resistance in

various pathogens.

This study aims to test the effectiveness of Grapefruit seed and Allium sativum

extract as a natural component in making antimicrobial foot cream to test for athletes’

foot.

2
Conceptual Framework

FEATURES
Eco-friendly
Organic material

Pulverized EFFECTIVENESS
Citrus x This antimicrobial foot
Paradisi seed cream no longer has
and Allium financial complications
in finding such
Sativum extract
treatment. It helps to
as an prevent the dry skin,
Antimicrobial cuts, sore and infection
FOOT CREAM with natural remedy.
Product
BENEFITS
Antimicrobial
Moisturizer
Anti-fungal

As you can see in the chart that have been presented our product will benefit you

in terms of antimicrobial, moisturizer and antifungal because of the materials that have

been used which is the Citrus Paradisi seed and Allium Sativum extract; the effectiveness

of the product depends on the main ingredients. Through the features of the product it

will become eco-friendly and will be able to adopt the better use of Grapefruit seed and

Garlic extract.

3
Statement of the Problem

This study about the “Citrus Pulverized Paradisi Seed and Allium Sativum Extract

as an Antimicrobial Foot Cream” aims to answer the following question:

1. How effective is the pulverized Grapefruit Seed and Allium Sativum Extract

in terms of:

A. Antimicrobial Property

B. Treating Athlete’s Foot

C. Duration

Hypothesis

As researcher of this study, it is highly shown that Null Hypothesis and

Alternative Hypothesis were drawn from the experimented and observation of the

material.

Ho: Null Hypothesis

The organic foot cream made of Grapefruit (Citrus x paradisi) and Garlic (Allium

sativum) is not effective as an antimicrobial and treating athletes’ foot.

Ha: Alternative Hypothesis

The organic foot cream made of Grapefruit (Citrus x paradisi) and Garlic (Allium

sativum) is effective as an antimicrobial and treating athletes’ foot.

4
Significance of the Study

The results and findings of these research will be going to benefit the community,

researchers and specially the student athletes. Through the pulverized grape fruit seed and

garlic extract as an anti-microbial foot cream, the student-athletes will be able to have

solutions that will able to help them treat their fungi, and other foot infections.

For the community who want a natural therapy to treat their fungal infections,

they will no longer have financial complications in finding such treatment for and buying

commercial products because these natural ingredients can be found anywhere. Simple at

may it seem but the community will be able to adopt the better use of grape fruit seed and

garlic extract.

For the respondents and future researchers, the study conducted will be the benefit

of them for it will bring them to deeper knowledge that garlic and grapefruit seed are not

waste, but a useful one in making remedies in treating fungal infections. It will feed their

minds that these simple materials contain natural components in making antimicrobial

foot cream.

Scope and Delimitation

This research study focuses on the research project on “Citrus Pulverized Paradisi

Seed and Allium Sativum Extract as an Antimicrobial Foot Cream” on how it can help

become a natural remedy in fighting fungal infections. There are factors that this study

5
considers such as the materials, the procedures, the utensils and ingredients needed on the

process of making antimicrobial foot cream. This study is limited only about the

components present in the organic materials that the researchers will use in making

antimicrobial foot cream. It also seeks knowledge about the experiences of self- foot care

and how do people manage their foot health.

The respondents of the study are limited only to 10 grade 12 students of Christ the

King College—San Fernando City, La Union Inc.

Definition of Terms

1. Grapefruit – it is a tropical citrus fruit known for its sweet and somewhat sour

taste containing vitamin C, antioxidize. It's rich in nutrients, antioxidants and

fiber. Its seed’s best defense against candidiasis is its antifungal properties.

2. Extract – it contains the active ingredient of a substance in concentrated form.

3. Antimicrobial – it is an agent that kills microorganisms or stops their growth

especially pathogenic microorganisms.

4. Pulverized – it is to press or crush something until it becomes powder or a soft

mass.

5. Foot cream – it contains some stabilizing and thickening ingredients. An

essential that helps to keep our feet in good form making them soft and keeping

them from getting cracks, dry skin, red spots and calluses.

6. Fungi – it is a wide variety of organisms that reproduce by spores, including the

mushrooms, molds, yeasts, and mildews. It feed off of dead organic matter or

living organisms.

7. Remedy – it is a treatment for disease or injury.

6
 CHAPTER II

METHODOLOGY

This part discussed the material and methods used as well as the entire procedures of the

study.

Research Design

This study entitled "Citrus Pulverized Paradisi Seed and Allium Sativum Extract

as an Antimicrobial Foot Cream" is an applied research that aims to solve problems

related to hygiene and sanitation through the use of organic materials. As Calmorin

(2010) stated, applied research is the type of research that involves seeking new

applications of the scientific knowledge to the solution of a problem such as the

development of a new system or procedure, new device, or new method in order to solve

problems. In this type of research, the problem is identified as new system or new method

is applied to solve the problem. On the other hand, experimental type of research is

conducted in this study for it aims to know the potential uses of Citrus Pulverized

Paradisi Seed and Allium Sativum Extract as an Antimicrobial Foot Cream.

Pre-Experimental Design is used to conduct the given study. This design is used

to determine the effectiveness of pulverized Grapefruit Seed and Allium Sativum Extract

in terms of Antimicrobial property and Treating athletes’ foot. This design is the basic

7
form of research design in which involves a single group or multiple groups that are

observed successively to some agent or treatment presumed to cause changes.

In finding the solution to the research problem, the researchers used the

quantitative analysis. It is a technique that seeks to understand behavior by using

mathematical and statistical modeling, measurement, and research. emphasize objective

measurements and the statistical, mathematical, or numerical analysis of data collected

through polls, questionnaires, and surveys, or by manipulating pre-existing statistical data

using computational techniques. Quantitative research focuses on gathering numerical

data and generalizing it across groups of people or to explain a particular phenomenon.

(Creswell, 2013).

Instrumentation and Data Collection

This study is a non-probability sample that is selected based on the

characteristics of the population and the objective of the study. It is an unbiased

surveying technique. Survey-questionnaire is conducted to gather reliable data, facts and

opinions from the respondents. Purposive sampling is also known as judgmental,

selective, or subjective sampling. This is the most common sampling strategy, useful in

situations when you need to reach a targeted sample quickly, and where sampling for

proportionality is not the main concern.

Tools for Data Analysis

The researchers gathered the data through a survey, Internet, books and other

related studies. From the observation collected in the conducted experiment, it was

tallied, examined and explicated. The data gathered from survey questionnaire are

necessarily needed to acquire and go deeper from the experiences of the respondents that

8
are subjected to data through analysis and interpretations. The pen-and-paper method was

used to enumerate and analyze the answer of the respondents through compare and

contrast method. The 2019 version of Microsoft Word.

Locale and Population of the Study

The respondents of the study are limited only to 10 grade 12 students of Christ

the King College—San Fernando City, La Union Inc.

Based on this purpose, a list of student athletes of Christ the King College-San

Fernando City, La Union Incorporated school year 2019-2020 was interviewed as the

researchers’ respondent. The student athletes can contribute to the study because they are

the people who are prone to have athlete’s foot. Answers will be gathered, tallied,

analyzed and interpreted. The researchers will be using the pulverized grapefruit seed and

allium sativum extract and selecting athlete students to observe and answer the survey

questionnaires prepared by the researchers.

Ethical Consideration

To establish and safeguard ethics in conducting this research, the researchers

strictly observed proper reference and the following:

The student athletes’ name will not be mentioned in any part of this research.

Proper document sourcing or referencing of materials was done to ensure and promote

copyright laws, this study will be constructed legally. They used proper citations to avoid

plagiarism and falsification.

Materials:

 Garlic Cloves - Garlic Extract

9
  Grapefruit seed - Pulverized

 Mortar and Pestle

 Beeswax (Multipurpose)

 Container

 Blender Grinder

Procedures:

1. Prepare all the ingredients needed.

2. Grind Garlic Cloves and pull the extract out of it using Cheese Cloth.

3. Weigh 70 grams of Grapefruit see and pulverized.

4. Separate the two samples into a clean container.

5. Use water bath to boil the product. Place the beaker just on top and put 30 grams

of beeswax, let it melt for a while and continue stirring well.

6. Add 5 grams of cornstarch and add drop by drop tea tree oil to the sample. Mix it

thoroughly.

7. Remove the product and place it into a clean container, let it cool for five minutes.

8. Allow to solidify the product. Test product by applying the infected part of the

feet.

10
 CHAPTER III

RESULTS AND DISCUSSION

This chapter presents the answer of the respondents in the survey conducted by

the researchers through graphs and interpretations.

There are ten grade 12 students’ total respondents from Christ the King

College—San Fernando City, La Union Inc. Sample products with the given to the

respondents as basis of their responses in the questionnaire.

The product was evaluated base on the effectivity of the product.

The charts presented shows that the inguinal, squamous, nose, ear and foot

bacteria are sensitive to the antibiotic discs out of guava leaves extract. None of them was

resistant.

RESULT
BATCH COMPONENT DESCRIPTION

15 grams of garlic extract Very rough, firm, uneven

8 drops of eucalyptus oil pulverized grapefruit seed,
I
7 grams of beeswax not compact, candle-like
5 grams of pulverized grapefruit seed texture, easy to apply

11
 15 grams of garlic extract
8 drops of eucalyptus oil
II
7 grams of beeswax
5 grams of pulverized grapefruit seed
15 grams of garlic extract
8 drops of eucalyptus oil
III
7 grams of beeswax
5 grams of pulverized grapefruit seed
15 grams of garlic extract
8 drops of eucalyptus oil
IV
7 grams of beeswax
5 grams of pulverized grapefruit seed
15 grams of garlic extract
8 drops of eucalyptus oil
V
7 grams of beeswax
5 grams of pulverized grapefruit seed
15 grams of garlic extract
8 drops of eucalyptus oil
VI
7 grams of beeswax
5 grams of pulverized grapefruit seed
15 grams of garlic extract
8 drops of eucalyptus oil
VII
7 grams of beeswax
5 grams of pulverized grapefruit seed
15 grams of garlic extract
8 drops of eucalyptus oil
VIII
7 grams of beeswax
5 grams of pulverized grapefruit seed
15 grams of garlic extract
8 drops of eucalyptus oil
IX
7 grams of beeswax
5 grams of pulverized grapefruit seed
12
Very rough, firm, uneven
pulverized grapefruit seed,
not compact, candle-like
texture, easy to apply
Very rough, firm, uneven
pulverized grapefruit seed,
not compact, candle-like
texture, easy to apply
Very rough, firm, uneven
pulverized grapefruit seed,
not compact, candle-like
texture, easy to apply
Very rough, firm, uneven
pulverized grapefruit seed,
not compact, candle-like
texture, easy to apply
Very rough, firm, uneven
pulverized grapefruit seed,
not compact, candle-like
texture, easy to apply
Very rough, firm, uneven
pulverized grapefruit seed,
not compact, candle-like
texture, easy to apply
Very rough, firm, uneven
pulverized grapefruit seed,
not compact, candle-like
texture, easy to apply
Very rough, firm, uneven
pulverized grapefruit seed,
not compact, candle-like
texture, easy to apply
 15 grams of garlic extract
8 drops of eucalyptus oil
X
7 grams of beeswax
5 grams of pulverized grapefruit seed
Very rough, firm, uneven
pulverized grapefruit seed,
not compact, candle-like
texture, easy to apply

The table indicates results of experiments that are conducted. The researchers

found out that that when 15 grams of garlic extract, 8 drops of eucalyptus oil, 7 grams of

beeswax, and 5 grams of pulverized grapefruit seed, the outcome became very rough, has

a firm and candle-like texture and turns out the product is not that compact but easy to

apply.

13
 Graph 1

Efficacy of Antimicrobial Property

YES NO

0.7

30%

YES NO

Grapefruit seed and Allium sativum extract has various properties for

antimicrobial resistance. In 10 respondents, seven out of ten respondents answered yes

(70%) and three out of ten answered no (30%). Therefore, based from the graph above, it

is long-standing that the grapefruit seed and allium sativum extract has effective

antimicrobial properties.

Kekuda, P., (2014) stated that Grapefruit seed is known to exhibit various

bioactivities. In the present study, it is determined that antimicrobial activity of

14
grapefruit seed. Poisoned food technique was performed to screen antifungal effect of

grapefruit seed. Grapefruit seed was effective against mycelia growth.

Yadav, S., (2015) stated that varying concentrations of fresh garlic extract were

tested for their antimicrobial activity against common pathogenic organisms and has an

important medicinal role for centuries. Its therapeutic uses include beneficial effects on

antimicrobial, antibiotic and anti-inflammatory.

The purpose of this research was to study Grapefruit seed (citrus paradisi) and

Garlic (allium sativum) extract as a functional ingredient for tinea pedis. The study used

beeswax; it creates a barrier which helps to seal moisture into the skin. This barrier also

helps to protect the skin from environmental toxins and irritants also a natural exfoliator,

ideal for sloughing away dead skin cells.

15
 Graph 2

Effectivity in Treating Athletes’ Foot

YES NO

0.6

40%

YES NO

The Grapefruit and Allium sativum extract are indeed effective in treating

athletes’ foot. There are 6 out of ten respondents (60%) who answered yes and for out of

ten respondents (40%) answered no, because it has no effect for them—they only got to

test a particular pathogen on their feet. However, the overall data concludes that the

grapefruit seed and allium sativum extract has the ability to eliminate fungal infections.

16
 Reagor, L., (2002) states that Garlic (Allium sativum) extract is one of the best-

researched herbal remedies and is also commonly used for treating various health

problems. Garlic is widely known for its biological properties and plays an important role

as an antioxidant. Garlic extract is considered as a good candidate in the search for an

antimicrobial agent because it has higher antibacterial activity against gram positive

bacteria compared to gram negative bacteria.

Harich, J., (2020) stated that Grapefruit seed is a naturally potent antioxidant

derived from the seeds and pulp. It is used as a natural preservative in a wide range of

cosmetic and skincare products to help stop the growth of harmful microorganisms such

as fungus or bacteria and extend its shelf life.

Gotter, A., (2019) stated that Grapefruit seed extract’s best defense against

candidiasis is its antifungal properties. Grapefruit seed extract have comparable

effectiveness in treating multiple types of bacteria strains as commonly prescribed topical

antibiotics. Athlete’s foot is a topical candida infection affecting moist areas on the feet.

Because of this, topical administration of grapefruit seed extract is best remedy for

treating as such.

17
 Graph 3

Duration
7
6
5
4
3
2
1
0
3 DAYS 5 DAYS 7 DAYS 10 DAYS

Column2

The respondents tested the duration of the product by checking it if it is effective

during the ten days of testing the antimicrobial foot cream. On Day 3 of testing the

product, 2 out of 10 respondents said that there are changes while using it. There are 6

out of 10 respondents said that on Day 5 of testing the product, they see positive

outcomes. On Day 7 and 10, there are at least 2 out 10 respondents said that they

observed changes on these days. This would mean that the product is effective on

different skin types of the feet that also work on different period of time.

18
 Athletes’ foot was derived from the increased occurrence of the condition among

athletes, because of their use of occlusive footwear, which create the ideal conditions for

dermatophyte reproduction. It is characterized by whitish macerated skin and itchy or

asymptomatic erythema between the toes, usually in the fourth and fifth spaces. Blisters

and cracks in the skin between the toes may cause pain and inflammation of the exposed

raw tissue (Macura 1993). Nuance, M. Q., (2000) stated that Aside from the modern

ways of treating Athlete's foot, many are resorting to the use of Garlic to control and

remedy Athlete's Foot and other fungus infection.

Graph 4

Evaluation of Odor and Complication

YES NO

100%

YES NO

The product does not have any foul smell. In general, 10 out of 10 (100%)

respondents that there is no change of the natural smell of their foot while using the

product and no complications all throughout the testing of Antimicrobial foot cream. 0

19
out of 10 respondents said otherwise. This means that the product does not possess

unpleasant smell and has no negative side effect on the respondents’ feet.

Rahman. M. S., (2007). Garlic (Allium sativum) extract is one of the main

ingredients of the product. Allicin is the dominant component of garlic. Along with its

decomposition products diallyl disulfide and diallyl trisulfide, are major contributors to

the characteristic odor of garlic, with other allicin-derived compounds, such as vinyl

dithiins and ajoene.

Grapefruit seed is rich in essential oils and antioxidants and has a variety of

potential health benefits. It contains powerful antimicrobials containing potent

compounds that can kill more than sixty types of bacteria and yeasts. Julson, E., (2018)

20
 Graph 5

Recommendable

YES NO

0.6

40%

YES NO

The product is recommendable to be used as an Antimicrobial foot cream. The

respondents are asked if they would recommend the product to other users of foot cream.

The graph presents that 6 respondents (60%) answered that they agree upon

recommending the product while there are 4 respondents (40%) answered that they are

not in favor of recommending the organic foot cream product. Most of the respondents

said that they would recommend the product to others. This means that it can be clearly

determined that the product is highly recommendable to be used as cheap alternative to

commercialized ones. Also, the respondent doesn’t find it difficult on applying the

product on the affected regions of their feet.

21
 Graph 6

Organic or Commercialized

Organic Commercialized

0.7

30%

ORGANI C C OMMER C I AL I ZED

The graph presents that 7 respondents (70%) answered that they are more in

favor of using the organic product for it is more affordable than the commercialized ones

while there are 3 respondents (30%) answered that they will remain and stick to the

commercialized products. Most of the respondents said that they would use organic foot

cream product. This means that it can be clearly set on that the organic product is still the

dominant one in use for treating infected foot. Also, without harsh chemicals, this organic

foot cream products are less likely to cause allergic reactions, inflammations or

irritations. If an allergic reaction does occur with the use of it, it would most likely be due

to a natural ingredient which would be easier to identify.

22
 CHAPTER IV

SUMMARY, CONCLUSIONS AND RECOMMENDATIONS

Summary

This study focuses on the effectiveness of pulverized citrus paradisi seed and

allium sativum extract as an antimicrobial foot cream. This study aims to gather

knowledge about experiences of foot self-care from the perspective of healthy people in

order to improve their welfare and their management of foot health. It desires to know the

advantages of the said main materials in management of foot health in terms of

antimicrobial property, treating athletes’ foot and duration. It this study it showcased and

proved the effectiveness of the product to become an antimicrobial foot cream by several

tests and experimentation.

The method used in this study was experimental wherein the researchers

underwent and performed experiments to create a product that will serve as a sample.

Survey questionnaires were made and distributed to gather data and evidences the

student athletes of Christ the king college San Fernando city la union Inc. were given the

opportunity were given the opportunity to try the given samples they were given the

chance to test the antimicrobial foot cream by putting it in their feet for (number of days)

consecutive days to test its effectivity in treating their athletes foot. The respondent

observes the result while answering the questionnaires.

Findings

The following are the relevant findings of the study found by the researchers:

1. Grapefruit seed and Allium sativum extract was effective in treating fungi.

23
 2. Grapefruit seed and Allium sativum extract is effective in eliminating bacteria in

the regions of feet.

3. Grapefruit seed and Allium sativum extract has the potential in making

antimicrobial foot application.

4. Grapefruit seed and Allium sativum extract is effective in common foot infection

caused by fungi in the most possible expected measure.

Conclusion

Athlete's foot (tinea pedis) is a fungal infection that usually begins between the

toes. It commonly occurs in people whose feet have become very sweaty while confined

within tightfitting shoes. Signs and symptoms of athlete's foot include a scaly rash that

usually causes itching, stinging and burning. Athlete's foot is contagious and can be

spread via contaminated floors, towels or clothing.

The researchers concluded that this study "Pulverized Grapefruit (Citrus x

paradisi) seed and Garlic (Allium sativum) extract as an antimicrobial foot cream" is

highly effective to eliminate fungal infection like athlete's foot.

The quality, texture, and appearance of the product produced greatly depending

on the proportions and quality of the additives and organic materials used. Step-by-step

procedure also contribute to the product's quality and composition.

24
Recommendations

Based on the findings and results of the study, here are several recommendations

to be considered:

1. This anti-microbial can easily rot, as a solution we can add some products that will

help the product to preserve and other solutions that have been observe during the

process in making the product.

2. Some of the respondents says that the product doesn’t have the good odor, as per said,

we can add some oil that have a good and strong scent.

3. The researchers should always follow the rules and regulation of the laboratories.

4. As a researcher, we would like to recommend this to the athletes because it can easily

made and we can find the materials needed in the market.

5. Smelly foot can lead to serious problem if not treated immediately. Grapefruit seed

and allium sativum extract are instant cure for getting rid of bad odor as they contain

tinea fungus that grows on feet.

25
 BIBLIOGRAPHY

Online Sources

Reagor, L., (2002, June 8). National Library of Health Medicine National

Institutes Review. Retrieved from

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3249897/

Kekuda, P., (2014, January). Ancient Science of Life. Retrieved from

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3336284/

Yadav, D., (2015, April). An International Quarterly Journal of Research in

Ayurveda. Retrieved from https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4784133/

Harich J., (2020, March 4). New Directions Aromatics Blog. Retrieved from

https://www.newdirectionsaromatics.com/blog/products/preservative-powers-of-

grapefruit-seed-extract.html

Gotter, A., (2019, February 20). Healthline. Retrieved from

https://www.healthline.com/health/grapefruit-seed-extract-candida

Nuance, M. Q., (2000). VisiHow See It Do It. Retrieved from

https://visihow.com/Eliminate_Fungal_Infections_and_Athletes_Foot_with_Garlic_Rem

edies

Rahman, M. S., (2007). Health Benefits and Bioavailability. Retrieved from

https://www.tandfonline.com/doi/pdf/10.1080/10942910601113327

Julson, E., (2018, June 29). Healthline. Retrieved from

https://www.healthline.com/nutrition/grapefruit-seed

26
27
Name (Optional): ________________________________________________________
Age: __________

INSTRUCTION: Write a small check [✓] inside the box that fits your answer. Please
write neatly and legibly.

QUESTIONS YES NO
Is the product effective in terms of
the following:
A. Antimicrobial Property
B. Treating Athletes’ foot
C. Duration
After using the product, did you
notice any changes in your foot
any changes in your foot in terms
of:
A. Odor
B. Color
C. Complication
Is the product recommendable to
use based on your experience?
Are you having difficulties
applying the product?
Do you think that the organic
product foot cream is better than
the commercialized product?

28
CHARISH MAE C. MEDIDAS
Zone 6, Langcuas, City of San Fernando, La Union
DOCUMENTATION

Prepare the laboratory equipment and materials needed for the experiment.

Pull out seeds from the Grapefruit and peel three Garlic cloves.

29
 Weigh the Garlic. Mince and grind the Garlic well using mortar and pestle.

Pulse the Garlic and pull the extract from it using Cheese Cloth. Store in Refrigerator.

30
 Gape Grapefruit detach seeds from it and eat the fruit. Set aside fruit seeds.

Dry seeds and Bake it in the oven. After baking it in the oven peel again seeds to get the

innermost part of the Grapefruit seed.

Set up a double-boiler. Place a saucepan filled with 2 inches of water on the stove. Place

a heat-safe, glass measuring cup into the water, then bring the water to a simmer.

Boil beeswax, carefully stir until substance yields to external pressure.

31
Allow set aside ingredients to join the beeswax and mix well.

32
 Stir mixture until ingredients becomes fully united and form viscous outcome.

From the beaker, when it is not too hot to touch, pour the mixture half-full to the half-

open petri dishes.

33
 Close them so that no other unrelated microorganism will exploit the product. Pour and

transfer the mixture in an air tight container for allow the mixture to store in a refrigerator

to solidify for testing.

After the experiment, discard the wastes properly into a bag, seal the waste bag and

dispose it. Wash the dishes and sanitize. Do the process twice thoroughly.

34