7.75743.0004-6013748955 msp.

December 2018

1.00857.0001 7. Procedure
Measuring range 1.1 - 107.0 mg/l SiO2 (0.5 - 50.0 mg/l Si):

Spectroquant ® Pretreated sample 4.0 ml Pipette into a test tube.

Silicate (Silicic Acid) Test Si (20 - 40 °C)

Reagent Si-1
Reagent Si-2
4 drops 1) Add and mix.
2.0 ml Add with pipette and mix.

Leave to stand for 2 min (reaction time A).

1. Method Reagent Si-3 4 drops 1) Add and mix.
In sulfuric solution silicate ions react with molybdate ions to form a yellow
heteropoly acid that is determined photometrically. Leave to stand for 2 min (reaction time B), then fill the measurement
The method is analogous to APHA 4500-SiO2 C. sample into a 10-mm cell, and measure in the photometer.

Hold the bottle vertically while adding the reagent!

1)

2. Measuring range and number of determinations

Cell Number of Measuring range 11 - 1070 mg/l SiO2 (5 - 500 mg/l Si):
Measuring range
mm determinations
Distilled water 1) 5.0 ml Pipette into a test tube.
1.1
- 107.0 mg/l SiO2 (20 - 40 °C)
11 - 1 070 mg/l SiO2 Pretreated sample 0.50 ml Add with pipette and mix.
10 100 (20 - 40 °C)
0.5 - 50.0 mg/l Si Reagent Si-1 4 drops Add and mix.
2)

5 - 500 mg/l Si Reagent Si-2 2.0 ml Add with pipette and mix.
For programming data for selected photometers / spectrophotometers see Leave to stand for 2 min (reaction time A).
www.service-test-kits.com.
Reagent Si-3 4 drops 2) Add and mix.
3. Applications Leave to stand for 2 min (reaction time B), then fill the measurement
Sample material: sample into a 10-mm cell, and measure in the photometer.
All kinds of water
It is recommended to use water for on-line analysis, Cat. No. 101051.
1)
Boiler water and boiler feed water
This test is not suited for seawater. Hold the bottle vertically while adding the reagent!
2)

Notes on the measurement:

4. Influence of foreign substances
This was checked individually in solutions containing 50 (500) and 0 mg/l
SiO2. The determination is not yet interfered with up to the concentrations
of foreign substances given in the table. The values in parentheses apply for
the measuring range 11 - 1070 mg/l SiO2. Cumulative effects were not
checked; such effects can, however, not be excluded.
Concentrations of foreign substances in mg/l or %
AsO43- 50 (500) NaCl 5%
Fe3+ 25 (250) NaNO3 10 %
P 100 (1000) Na2SO4 2.5 %
• Certain photometers may require a blank (preparation as per
measurement sample, but with distilled water instead of sample).
• For photometric measurement the cells must be clean.
Wipe, if necessary, with a clean dry cloth.
• Measurement of turbid solutions yields false-high readings.
• The pH of the measurement solution must be within the range
1.3 - 1.5.
• The color of the measurement solution remains stable for 15 min after
the end of the reaction time B stated above. The measurement value
subsequently diminishes slowly.

5. Reagents and auxiliaries 8. Analytical quality assurance

recommended before each measurement series
Please note the warnings on the packaging materials! To check the photometric measurement system (test reagents, measure-
ment device, handling) and the mode of working, a dilute silicon standard
The test reagents are stable up to the date stated on the pack when
solution containing 50.0 or 500 mg/l SiO2 (23.4 or 234 mg/l Si) can be
stored closed at +15 to +25 °C.
used.
Sample-dependent interferences (matrix effects) can be deter-
Package contents:
mined by means of standard addition.
1 bottle of reagent Si-1
Additional notes see under www.qa-test-kits.com.
1 bottle of reagent Si-2
For quality and batch certificates for Spectroquant® test kits see the
1 bottle of reagent Si-3
website, where you will find all data in production control, that are
2 AutoSelectors
determined in accordance with ISO 8466-1 and DIN 38402 A51.
Other reagents and accessories:
MQuant® Universal indicator strips pH 0 - 14, Cat. No. 109535 9. Notes
MQuant® pH-indicator strips pH 0 - 6.0, Cat. No. 109531
Sodium hydroxide solution 1 mol/l Titripur®, Cat. No. 109137 • Reclose the reagent bottles immediately after use.
Sulfuric acid 0.5 mol/l Titripur®, Cat. No. 109072 • Information on disposal can be obtained at
Water for on-line analysis, Cat. No. 101051 www.disposal-test-kits.com.
Silicon standard solution Certipur®, 1000 mg/l Si, Cat. No. 170236
Pipettes for pipetting volumes of 0.50, 2.0, 4.0, and 5.0 ml
Rectangular cells 10 mm (2 pcs), Cat. No. 114946

6. Preparation
• Analyze immediately after sampling.
• The pH must be within the range 2 - 10.
Adjust, if necessary, with sodium hydroxide solution or sulfuric acid.
• Filter turbid samples.

Merck KGaA, 64271 Darmstadt, Germany,

Tel. +49(0)6151 72-2440
www.analytical-test-kits.com
EMD Millipore Corporation, 400 Summit Drive
Burlington MA 01803, USA, Tel. +1-978-715-4321