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Section 4 Adjustment: XS Series S/M December 13, 2007
Section 4 Adjustment: XS Series S/M December 13, 2007
4.1
U OPTICAL ALIGNMENT ............................................................. 1
U
4.2
U Mechanical Adjustment ............................................................ 23
U
4.1.1
U Required Tools .................................................................... 1
U
4.2.1
U Piercer Position Adjustment .............................................. 23
U
4.1.2
U Optical Alignment Outline ................................................... 1
U
4.2.2
U Adjusting Rinse Cup Position ............................................ 23
U
4.1.3
U Replacing Flow Cell ............................................................ 2
U
4.2.3
U Adjusting Aspiration Position ............................................. 24
U
4.1.4
U Removing Photo Detector No.30 ........................................ 4 U
4.2.4
U Adjusting RBC Chamber ................................................... 24
U
4.1.5
U Removing XS_FCM Detector Block Cover B ...................... 4 U
4.2.5
U Adjusting Reaction Chamber............................................. 25
U
4.1.6
U Removing Flow Cell Unit .................................................... 5
U
4.2.6
U Sampler Adjustment .......................................................... 26
U
4.1.7
U Adjusting Laser Power ........................................................ 7
U
4.3
U Hydraulic Adjustment ............................................................... 30
U
4.1.8
U Adjusting Laser Diode Angle (when replacing Laser Diode)9 U
4.3.1
U Adjusting RBC Clog Level ................................................. 30
U
4.1.9
U Installing Flow Cell Unit No.11 .......................................... 10
U
4.3.2
U Adjusting HGB Blank ......................................................... 30
U
SECTION 4 ADJUSTMENT
4.1 OPTICAL ALIGNMENT
(3) Adjust the initial position of the LD unit. (4.1.12 (8) Check FSC signal using an oscilloscope. (4.1.19 Optical
Confirmation/Adjustment of Laser Diode Initial Position) Alignment (Rough)) Adjust Z-direction of LD unit so that FSC
1) Z-direction rough adjustment by aligning the corner of the laser signal width will become narrower than specified limit.
diode cylinder and laser diode base.
2) X-direction rough adjustment by aligning optical base slotted
portion and LD base.
XS Series S/M 4-1
(9) Adjust following units by observing SFL signal using an 4.1.3 Replacing Flow Cell
oscilloscope. Verify the results in Fine mode menu, and then fix
the units. (4.1.20 Optical Alignment (fine)) (1) Disconnect tubing from nipple on SV 11.
Cautions)
In case LD unit or Flow Cell angle adjustment is not well done,
uniform signal will be seen for a short time only (unstable).
If it is difficult to get SFL signal, check if Flow Cell image at the slit
of the SSC Detector is clear and sharp when shining a flash light
toward flow cell. If NOT, adjust SFL Lens X-direction to get sharp
and clear image on the slit.
(10) Adjust the SSC Detector left or right so that Flow Cell image can
be aligned at the slit of SSC Detector. (Step (9) in 4.1.20 Optical
Alignment (fine)) 11
(11) Adjust X-direction of the FSC Detector so that FSC signal
becomes the lowest. (This means direct laser beam is
interrupted at the beam stopper.) And then, fix the FSC Detector.
(Step (14) in 4.1.20 Optical Alignment (fine))
Caution)
Adjustment for FSC(W) data is not required as long as FSC(W) Valve Unit
data is within the specified limit.
6B
(1) Remove Photo Detector No.30 by loosening fixing screws (1) Remove XS_FCM Detector Block Cover B by loosening fixing
(Screw Binding M3X6). Cut a tie wrap tying wiring cord. screws (Screw Binding M3X6).
Disconnect the connector from the PCB No.2168.
Fixing Screw
Fixing Screw Photo Detector No.30
PCB No.2168
Disconnect
8B
Fixing Screws
9B
Disconnect
(5) When below screen appears, select “Ignore all Errors except
Sampler Errors” and click OK.
Close
1B
VR1
(3) Place a hex wrench between flow cell and laser diode, and
adjust the laser diode angle so that the reflected light and laser
light are one line.
(1) Tighten temporary the Flow Cell Unit No.11 with Screw (1) Double-click Laser Power on the service screen to activate
Hex-Socket Bolt M3X8. laser.
(2) Place a hex wrench between flow cell and laser diode, and
adjust the flow cell angle so that the laser beam and reflected
beam from the flow cell on the wrench are one line.
(3) Tighten fixing screws so that the flow cell angle doe not move.
(4) Confirm that the laser beam and reflected beam on the wrench
are one line after tightening the fixing screws.
13B
(5) Click OK on the Laser Power screen to turn off the laser.
14B
(1) Tighten fixing screws so that tubing of the bottom of the flow cell (1) Confirm/Adjust the Laser Diode initial position (forward/backward)
is parallel to the bottom surface of the FCM Detector Block as follows.
Cover B.
Loosen these
screws when
moving cylinder
F/B
F/B Adjustment
Screw
15B
Block
Surface
Laser Diode
R/L Fixing
Screws
Aligned
Do Not Loosen Fixing Screws
(2) Place a white paper as shown and adjust Laser Diode R/L
position so that two lines of flow cell are both sides of the beam
by turning adjustment screw.
(1) Click OK on the Laser Power screen to turn off the laser. (1) Double-click Laser Power on the service screen to activate
laser.
(2) Fix the Photo Detector Block No.30 so that the distance
between flow cell and Photo Detector Bloc No.30 is 7 mm by (2) Adjust beam stopper position so that the beam image is on the
tightening screw binding M3X6. center of the beam stopper as shown.
(3) Click OK on the Laser Power screen to turn off the laser.
18B
(1) Confirm/Adjust the adjustment plates position as follows. (Loose (1) Confirm/Adjust the Photo Detector No.28 initial position as
fixing screw when moving adjustment plates.) follows. (Loosen fixing screws (Screw Hex-Socket Bolt M3X6)
when moving Photo Detector No.28.)
Fixing Screws
(1) Remove rear panel. (1) Select Menu Æ Controller ( Maintenance ( Auto Rinse.
(2) Connect probes of the oscilloscope as shown. (Both FSC and SFL (2) Confirm that no background error is generated after performing
should be connected because SFL signal is triggered by FSC Auto Rinse.
signal.)
(3) Click Close to close the screen.
Warning
Since undiluted latex is easy to coagulate, shake it enough and add
FSC(1) GND SCC SFL to CELLPACK.
Use the latex materials within 3 hours after making it. Do not add
GND latex to made latex materials after a time. (Use the latex materials
within an hour after making for confirmation of Optical Alignment
(fine))
Since the fluorescence intensity becomes weak when A-7312 (latex
for fluorescence) receives light, store A-7312 in dark place. Use
diluted latex in a bottle.
Store latex in room temperature and do not freeze it.
(7) Adjust laser diode position so that the FSC wave on the
oscilloscope is highest and narrowest by turning adjustment screw
A and B. (9) Adjust FSC(W) approximately 0.3 or lower. If the value does not
become 0.3, repeat the steps (6) to (8).
(8) Adjust the beam stopper R/L position so that FSC wave is lowest
by turning adjustment screw C.
(11) Adjust Photo Detector position so that the SFL wave on the
oscilloscope is highest by turning adjustment screw D.
(12) Adjust Photo Detector Position so that the SFL wave on the
oscilloscope is highest and minimum variation by turning
adjustment screw E and F.
F E
(9) If the value is out of the range, repeat steps (5) – (8).
(1) Double-click Optical Axis Adjustment (fine) in service.
X W
(2) Pour 1 mL of FSC latex to reaction chamber and press start FSC 60 ± 30 ≤ 0.35
switch. SFL 130 ± 50 ≤ 0.30
SSC 50 ± 40 ≤ 0.35
(3) Enter appropriate value into Gain and click Send so that the
FSC(X) value is within the below range.
FSC(X): 60 +/- 30ch
(4) Run FSC latex three times after adjusting Gain and confirm that
the FSC(W) is within the below range.
FSC(W)≤0.35
(6) Enter appropriate value into Gain and click Send so that the
SFL(X) value is within the below range. Leaf spring for Laser Diode Laser Diode base
SFL(X): 130+/-50ch
(7) Run SFL latex three times after adjusting Gain and confirm that
the SFL(W) is within the below range.
SFL(W)≤0.30
Mirror
Shine with a flashlight Place the slit in center Fix the Photo Detector No.29
of the flow cell shadows while pressing to the base
4.2.1
24B Piercer Position Adjustment (1) Click Init. Position and adjust the piercer height so that the
piercer tip and Rinse Cup bottom are same height by clicking Up
(1) Replace adapter with XS Adapter (13 mm diameter) (P/N: or Down key.
442-3086-2.
(1) Click Aspiration Position and adjust the piercer position so that (1) Click RBC/HGB Chamber and adjust the piercer position so that
the piercer tip is as shown position by clicking Up, Down, Front the piercer tip is in the center of the chamber by clicking Up, Down,
and Back key. Front and Back key.
Same
height
with the
top
surface
Center
Adjust the center at Aspiration Position Same height with the top surface
Center
27B
(1) Click WBC Chamber and adjust the piercer position so that the
piercer tip is in the center of the chamber by clicking Up, Down,
Front and Back key.
Center
Same height with the top surface (2) After adjusting, click Save and Close.
(4) When movement completes, click Start in the Tube Rotation. Adust
the Sample Set Unit position so that the test tube rotates by
clicking Back and Front key. Adust the Sample Set Unit position so
that the test tube rotates discontinuously by clicking Front key.
Move Sample Set Unit backward – 15 pulses by clickin Back key.
Confirm that the test tube rotates continously. Click Save for
saving the position. Click Stop in the Tube Rotation and Close key.
BR Unit No. 1
(6) Tighten left fixing screw of the BR Unit No. 1. Click Close to close
the window. Tighten right fixing screw of the BR Unit No.1
(8) Adjust Hand height position so that the gap between rack and hand
is 1 to 2 mm by adjusting rod height position.
(10) After completing adjustment, click Save Æ Up and remove the test
tube from the Hand.
RACK 01
RACK-02
(12) After completing adjustment, click Save Æ Up and remove the test (14) After completing adjustment, click Save Æ Up and remove the test
tube from the Hand. tube from the Hand.
4.3.1
30B Adjusting RBC Clog Level Note: Digital VR values inversely adjust blank values, i.e., increasing
digital VR value, decreases HGB blank value.
(1) Select Menu Æ Controller Æ Service Æ RBC Clogs Level.
(1) Confirm that 30 minutes past after turning on the main unit.
(2) When “RBC Clog Error” occurs, click Close to close error
screen. (2) Select Menu Æ Controller Æ Service Æ Sensitivity.
(3) RBC Clog Level appears in real time. (3) Enter appropriate values into HGB blank and ASP. Sensor blank,
and click Send. Repeat this step until value comes within
(4) Enter “99” into Target box and click “>>>” ( “Send” (”Continue”. Adjustment Value range as shown in table below.
(5) Repeat step (4) until RBC Clogs is 99 +/- 1. Click Close when RBC
Clogs becomes 99 +/- 1.
(4) Click Close after values come within Adjustment Value range as
shown in table above.
(7) Click Close when value becomes HGB assay value for e-CHECK
Level 2 X 105 +/- 50.
CTRL3 appears
4.3.4 Adjusting Sensitivity (7) When the analysis is over select Sensitivity and confirm that
values are within the following range.
(1) Confirm that Aspiration Sensor adjustment is completed. (Select
Menu Æ Controller Æ Service Æ Mode. Select Control 3 in
Analytical Mode.) Target range
DIFF-X: assay value +/- 3ch
(2) Run e-CHECK (level 2). DIFF-Y: assay value +/- 2ch
Note: For American Markets, the X-CAL product is used rather FSC-X: assay value +/- 2ch
than e-CHECK level 2 when performing Sensitivity MCV: assay value +/- 1 fL
adjustments. MPV: assay value +/- 1fL
HGB: assay value +/- 0.2 g/dL
(3) Select Sensitivity in Service screen. (e-CHECK cannot be run in
the Sensitivity screen.)
(4) Enter each assay value in the Target box and click >>> and Make sure that the DIFF scattergram and FSC histogram are
Send. discrete and displayed normally.
Enter assay value Click >>>
(8) Click Close when the values are within the range.
(9) Repeat step (6) and (7) until the values are within the range.
Click Send For adjusting DIFF-Y using human blood, aspirate ten normal blood
samples and Calculate mean value. Enter target value in DIFF-Y and
click ‘Send’ so that the result value will be in the following target value
range.
Target Value: 43.5 +/- 3.0
4.4.1
34B PCB No.6365T2 VR1: VR for LD power adjustment Increases LD power by turning to
CW.
(1) Dip Switch VR2: VR for high frequency overlap adjustment Increases high
frequency overlap.
bit ON OFF Default
8 Not used Not used OFF TP1: Ground for LD drive
7 Not used Not used OFF
TP2: Power output for LD Shows proportional voltage for LD power
6 Not used Not used OFF
5 Not used Not used OFF between TP2 and TP1. Proportional factor varies to laser
4 Initialize setting data in Not Initialize setting data in OFF diodes.
EEPROM at power ON EEPROM at power ON TP3: LD current monitor Shows following voltage.
3 Not used Not used OFF Voltage between TP3 and TP1=25 X LD current (example:
2 Not used Not used OFF
1 Initialize counters in Not Initialize counter in EEPROM OFF
2.5 V when LD current at 100mA)
EEPROM at power ON at power ON
35B