Hydraulics

SECTION 2 HYDRAULICS
2.1 PNEUMATIC SYSTEM............................................................ 2

2.2 USE OF PRESSURE AND VACUUM ..................................... 2
2.3 HYDRAULIC SYSTEM ............................................................ 2
2.3.1 RBC/PLT Analysis Procedure.................................4
2.3.2 Hgb Analysis Procedure .........................................5
2.3.3 Computing the Erythrocyte Indices .........................5
2.3.4 WBC Classification (CBC+DIFF mode) ..................6
2.3.5 WBC Classification (CBC mode) ............................7
2.4 ANALYSIS PARAMETER ........................................................ 8
2.5 REAGENT SYSTEM................................................................ 9
2.6 ANALYSIS FLOW .................................................................. 10
2.7 FCM DETECTOR BLOCK ..................................................... 11
2.7.1 Function................................................................12
2.8 OPTICAL SYSTEM................................................................ 14
2.9 HYDRAULIC PARTS ............................................................. 15
2.9.1 Solenoid Valve Function .......................................15
2.9.2 HYDRAULIC PARTS LOCATION .........................18
XS-Series S/M December 13, 2007

SECTION 2 HYDRAULICS 2.3 HYDRAULIC SYSTEM
a. Whole Blood Mode
2.1 PNEUMATIC SYSTEM
The pneumatic system needed for XS operation consists of 2 pneumatic
outputs, +0.06Mpa pressure, and -0.03Mpa vacuum.
These pneumatic outputs are generated by built-in air pump unit and
regulated to +0.06 Mpa and -0.03 Mpa by corresponding relief valves
respectively. These relief valves have no adjustment points.
Relief Relief
Valve Valve
Trap
Chamber Air Tank
Air Pump
Air Tank
-0.03Mpa +0.06Mpa
Figure2-3-0-1: XS Pneumatic System
2.2 USE OF PRESSURE AND VACUUM

Uses of pneumatic outputs for XS are as follows.
Type Functions
Pressure Driving diaphragm pumps, To form Sheath Flow, Figure 2-3-0-1: Hydraulic System Flow in Whole Blood Mode
0.06MPa Draining Pressure for Waste Chambers
Driving air cylinder in Sampler Unit
Vacuum Driving diaphragm pumps, Replenishing EPK Chamber,
-0.03MPa Draining Vacuum for Waste Chambers
Driving air cylinder in Sampler Unit
XS-Series S/M 2-2 December 13, 2007

b. Capillary Mode
Figure 2-3-0-2: Hydraulic System Flow in Capillary Mode

2.3.1 RBC/PLT Analysis Procedure 1. Sample Aspiration Probe aspirates whole blood (or diluted sample for
The procedure for analyzing RBC/PLT is explained here. capillary mode) .
2. 4.0µL of whole blood (9.0µL of diluted sample for capillary mode) is
diluted with 2.0mL of EPK (CELLPACK) in the RBC Chamber.
3. The Sheath Syringe injects 10.3µL of diluted sample slowly to the
RBC/PLT detector while RBC sheath flow is formed.
4. The RBC detector counts the RBC and PLT via the Hydro Dynamic
Focusing (DC Detection). At the same time, the hematocrit (HCT) is
calculated via the RBC pulse height detection method.
Figure 2-3-1: RBC/HGB Analysis Procedure

2.3.2 Hgb Analysis Procedure 3. The concentration of SLS-hemoglobin is measured as light
The procedure for analyzing HGB is explained here. absorbance, and is calculated by comparison with the absorbance of
the Hgb blank level measured in step2.
2.3.3 Computing the Erythrocyte Indices

The red blood cell constants (mean cell volume, mean cell hemoglobin,
and mean cell hemoglobin concentration) are calculated from the RBC,
HGB, and HCT.
1. Mean cell volume (MCV)
The MCV is calculated from the RBC and HCT, using the following
equation:
HCT(%)
Figure 2-3-2: Hgb Analysis Procedure MCV( fL ) = X10
RBC( x10 6 / μL )
1. After RBC/PLT analysis, 0.5mL of SULFOLYSER is added to 1.0 mL 2. Mean cell hemoglobin (MCH)
of diluted sample remained in RBC/HGB chamber, diluting to 751 The MCH is calculated from the RBC and HGB, using the following
times (2340 times for capillary mode), hemolyzing red blood cells, equation:
HGB( g / dL )
and converts hemoglobin to SLS-Hemoglobin. MCH(pg) = X10
RBC( x10 6 / μL )
2. The diluted sample in the step 1 is charged into the HGB flow cell.
3. Mean cell hemoglobin concentration (MCHC)
The MCHC is calculated from the HCT and HGB, using the following
equation:
HGB( g / dL )
MCHC( g / dL ) = X100
HCT(%)

2.3.4 WBC Classification (CBC+DIFF mode) 1. A fixed amount of 11µL of blood (55.5µL of diluted sample for
White blood cells (leukocytes) can be broadly classified as lymphocytes, capillary mode) is diluted by 1.0mL of STROMATOLYSER-4DL in the
monocytes, or granulocytes. Granulocytes can be further classified as WBC chamber.
neutrophils, basophils, or eosinophils, depending on the dye-affinity of At the same time, 30µL of STROMATOLYSER-4DS is added to stain
the granules. The applicable analysis procedure is explained here. the sample to a dilution ratio of 1:94. (1:94 in capillary mode) After
A 5DIFF analysis is used to identify and analyze the following white cell reacting for about 22 seconds, the red blood cells are hemolyzed and
groups: lymphocytes, monocytes, eosinophils, neutrophils, and the white blood cells are stained.
basophils. The 5DIFF analysis procedure is explained here. 2. The sheath syringe injects 95µL of diluted sample into the FCM
flowcell.
3. In the FCM flow cell, the sample is analyzed via flow cytometry
method utilizing a semiconductor laser.
Figure 2-3-4: WBC/DIFF Analysis Procedure

2.3.5 WBC Classification (CBC mode)
Figure 2-3-5: WBC (CBC8) Analysis Procedure
1. A fixed amount of 11µL of whole blood (55.5µL of diluted sample for

capillary mode) is dispensed into WBC chamber, diluted, and
hemolysed with 1.0mL of STROMATOLYSER-4DL.

2.4 ANALYSIS PARAMETER
Table 2-4-1: Analysis Parameters (RBC, HGB)
Analysis Block RBC/PLT HGB

Principle Sheath Flow DC Detection Method Colorimetric
Analysis Channel RBC/PLT Channel HGB Channel
Information PLT Histogram RBC Histogram
Parameter PLT HCT RBC HGB
MCV,MCH,MCHC,
Analytical Parameter MPV,PDW, P-LCR RDW-SD/CV
Reagent CELLPACK SULFOLYSER
Table 2-4-2: Analysis Parameters (WBC)
Analysis Block WBC

Principle Flow Cytometry by using Semi-Conductor Laser
Analysis Channel WBC Channel 5DIFF Channel
Information WBC Histogram 5DIFF Scattergram
Parameter WBC# BASO% Lymph% MONO% NEUT% EOSI%
BASO# Lymph# MONO# NEUT# EOSI#
Reagent STROMATOLYSER-4DS STROMATOLYSER-4DS Lysing solution/ Staining Dye

2.5 REAGENT SYSTEM
STOROMATOLYSER-4DL
(Lysing solution) WBC#
STOROMATOLYSER-4DS LYMPH#,%
(Dye)
MONO#,%
EOSI#,%
NEUT#,%
BASO#,%
RBC#
CELLPACK HCT
PLT Analyzed
Data
SULFOLYSER HGB
Figure 2-5 Reaction Chamber Construction

2.6 ANALYSIS FLOW
WBC Channel
Detector RBC/PLT HGB WBC/DIFF
Analysis Hgb
Channel RBC/PLT Channel Channel WBC Channel DIFF Channel
Detection SLS
Method Seath Flow DC Method
Hemoglobin
FCM (Semi-Conductor Laser)
PLT RBC WBC Histgram DIFF Scattergram

Information Histogram Histogram
WBC# Lym% BA% MO% NE% EO%

Parameter PLT HCT RBC HGB
Lym# BA# MO# NE# EO#
MPV MCV
RDW-SD
PDW RDW-CV MCH
P-LCR MCHC
STROMATOLYSER-4DL
Lysing solution
Reagent CELLPACK Ⅱ SULFOLYSER STROMATOLYSER-4DS

Dye

2.7 FCM DETECTOR BLOCK
CAUTION:
Laser Diode is very sensitive to static electricity. Follow the
PCB No. 2168 Collector Lens Unit Photo Detector instruction below when you replace the Laser Diode.
No.28 - To prevent electrostatic discharge damage, touch the
chassis to establish a ground before servicing Optical Unit
Photo Detector
No.29 No.22.
- Remove jumper pin from the terminal on the Laser Diode
AFTER connecting to PCB No. 2168. If the jumper pin is
Laser Diode
removed before connecting to PCB No. 2168, Laser Diode
Photo Detector
No.30 will be damaged.
- Do not disconnect the connector to the PCB No. 2168.
In the worst case, the device inside the Laser Diode will be
destroyed.
Beam Stopper
Flow Cell - Before reapplying the power, be sure that VR-1 on PCB No.
2168 is turned all the way CCW.
PCB No. 2168

Figure 2-7-0: FCM Detector Block
Laser Diode
The life of the Laser Diode is approx. 10,000 hours.

2.7.1 Function Following table shows which receiver is used for each parameter.
(1) Outline
Laser diode (Wave length: 633 nm) apply to samples in sheath flow FSC Side Scattered Side Fluorescence
generated flow cell. FCM Detector Block detects front scattered Photodiode Photodiode APD
light, side scattered light, and side fluorescence light from these WBC# / BASO Used Used Not Used
samples. XS generates two-dimensional scattergrams from these DIFF Used but not Used Used
signals as follows. displayed in
Scattergram
X-axis Y-axis (2) Beam spot generating system
WBC# SSCL FSCP Beam spot generating system consists of laser diode, collimator lens.
DIFF SSCL SFLP Laser beam generated by laser diode is converted to parallel beam
SSCL: Side Scattered peak Logarithm by collimator lens. This beam is collected by condenser lens and
FSCL: Front Scattered peak Logarithm applies to samples. Beam shape is as follows.
SFLP: Side Fluorescence Light Peak linear
10 µm
SFLL: Side Fluorescence Light peak Logarithm
FSCP: Front Scattered Peak linear
220 µm
(3) Sheath flow system
Sheath flow is generated in the flow cell. Sample flows center of
the flow cell.
(4) Front scattered light system

Front scattered light system consists of beam stopper and FSC
condenser lens. Beam stopper shade direct light from the laser
diode. FSC condenser lens collect the scattered light of the sample.

(5) Dichroic mirror
Light below 640 nm wave length reflects to the side scattered light
APD.
(6) Side fluorescence light system

Side fluorescence light system consists of interference filter, APD
(Avalanche Photodiode). The light through dichroic mirror is filtered
by interference filter and fluorescence light over 660 nm wave length
is detected by APD. Detected fluorescence light signal is amplified
by pre-amplifier and transmitted to main amplifier.
(7) Side Scattered light system

The scattered light reflected by dichroic mirror is detected by
photodiode. Detected scattered light signal is amplified by
pre-amplifier and transmitted to main amplifier.
(8) Cover switch

Cover switch is installed to prevent interference from the outer light
and leakage of the laser beam. If the cover is opened, XS stops
laser radiation and a message is displayed.

2.8 OPTICAL SYSTEM

2.9 HYDRAULIC PARTS
2.9.1 Solenoid Valve Function
Following 2 types of direct operated solenoid valves are used in the XS hydraulic system.
2-way type: 3-way type:
2 each of 2-way solenoid valves are built-in a module as shown below. 2 each of 2-way solenoid valves are built-in a module, and one port of
Each S.V. operates independently. each SV is connected as common port internally. The entire module
functions as a 3-way valve. Each SV has own driver, therefore, the SV
No. is assigned separately. See the figure below.
SV1
B A B
SV2
A A
A B
C C D B
D C C
Figure 2-10-1: 2-Way/3-Way SV Modules Used in XS Hydraulic System

Refer to the following table for the solenoid valve types.
Table 2-9-1: List of Solenoid Valves (in order of SV No.)
SV Function Located 2-way/ Diaphragm Material Ref.
No Unit 3-way Page
1 WB Asp Sensor Charge Valve No. 2-way EPDM 2-21
2 RBC/HGB Chamber Drain 182 Assy
3 Sheath Syringe Rinse Valve No. 3-way EPDM 2-21
4 RBC Back Flush 182 Assy
5 RBC Front Flush OUT Valve No. 3-way EPDM 2-21
6 RBC Sheath Flow 182 Assy
7 RBC Front Flush IN Valve No. 3-way EPDM 2-21
8 Rinse Cup Flush 182 Assy
9 EPK DP Replenish Valve No. 2-way EPDM 2-21
10 WBC/DIFF Chamber Drain 182 Assy
11 FCM Sheath Flow Valve No. 2-way EPDM 2-21
12 WBC/DIFF Sample Charge-1 182 Assy
13 Sample Charge DP Drain Valve No. 3-way EPDM 2-21
14 HGB Sample Charge 182 Assy
15 EPK Chamber Replenish Valve No. 2-way EPDM 2-21
16 RBC Sample Charge-1 182 Assy
17 EPK line air bubble removal Valve No. 2-way EPDM 2-21
18 Open EPK_DP discharge line to RBC/HGB chamber 182 Assy
19 Waste chamber pressure line Valve No. 3-way EPDM 2-21
20 Waste chamber vacuum line 182 Assy
21 Air supply to CP needle (CP model only) Valve No. 2-way EPDM 2-21
22 Piercer needle internal rinse line open 182 Assy
23 DIL chamber pressure line (sheath pressure for RBC, DIFF analyses) Valve No. 3-way EPDM 2-21
24 DIL chamber vacuum line (for diluent replenish) 182 Assy
25 (reserved) Valve No. 2-way EPDM 2-21
26 (reserved) 182 Assy
(To be continued)

Table 2-9-1: List of Solenoid Valves (in order of SV No.) (Continued)
SV Function Located 2-way/ Diaphragm Material Ref.
No Unit 3-way Page
27 FFD (DIFF lyse) DP dispense Hydraulic 3-way EPDM 2-20
28 FFD_DP replenish line open Unit-C Assy
29 Pressure supply to all DPs Hydraulic 3-way EPDM 2-20
30 Vacuum supply to all DPs Unit-C Assy
31 SLS (HGB lyse) DP dispense Hydraulic 3-way EPDM 2-20
32 SLS_DP replenish line open Unit-C Assy
33 DIFF sheath syringe switching FCM 2-way EPDM 2-20
34 DIFF charging line Reaction
Unit
35 FFS (DIFF dye) DP dispense Hydraulic 3-way EPDM 2-20
36 FFS DP replenish Unit-B Assy
37 RBC sheath syringe switching RBC Valve 2-way EPDM 2-20
38 RBC charging line Assy

2.9.2 HYDRAULIC PARTS LOCATION
Hydraulic
Unit-C Assy Hydraulic
FCM Detector Unit-B Assy
Block
Whole Blood
Pump
Diluent Chamber Assy Sample Aspiration

Hydraulic Sensor
Unit-A Assy (in XS_HGB_Assy)
Sheath Syringe
Hgb Flow Cell

(in XS_HGB_Assy)
Figure 2-10-2-2: Front Interior

Valve No. 182 Assy
Figure 2-10-2-1: Left Side Interior

Trap Chamber
Air Pump Assy No.9 Comp Assy
FCM Reaction Unit
CP Assy
RBC Detector Block
Sample Set Unit

RBC Valve Assy
Figure 2-9-2-3: Right Top Interior

3-Way Solenoid
Relief Valve Relief Valve Valve SV27 – 32
Bubble Sensor Bubble Sensor
(Vacuum) (Pressure) for SLS reagent for FFD reagent
27 29 31
Waste Chamber
WC-1 28 30 32
Trap Chamber
Waste Chamber
WC-2
Pinch Valve 1
Pinch Valve 3
Rinse Cup drain
WC-2 drain
Diaphragm Pump Diaphragm Pump
for SLS reagent for FFD reagent
Pinch Valve 2 (0.5 mL)
WC-1 drain (0.5 mL）
Figure 2-9-2-6: Hydraulic Unit-C Assy

(View from internal side)
Figure 2-9-2-4: Hydraulic Unit-A Assy
3-Way Solenoid
Valve SV35, 36 Bubble Sensor
35 for FFS reagent
36
Diaphragm Pump
for FFS reagent
(30 µL)
Figure 2-9-2-5: Hydraulic Unit-B Assy

SV21 – 26
25
23
RBC Detector 21
26
Block 24
Isolation
IsolationChambers
Chamber (Sheath Detector) 22
SV11 – 20
19
17
15
20 13
18 11
16
14
12
SV1 – 10
9
7
10 5
8 3
1
6
Diaphragm Pump 4
Figure 2-9-2-7: Detector Block for EPK (1.0 mL) 2
Diaphragm Pump
for Sample Charge
(1.0 mL)
Figure 2-9-2-8: Valve No. 182 Assy

Figure 2-9-2-9: Air Pump (No. 1 Assy)
Figure 2-9-2-11: CP Assy
Air Tank (Vacuum)

Air Tank
(Pressure)
Diluent (EPK)
Chamber
Figure 2-9-2-10: Diluent Chamber Assy

Figure 2-9-2-12: Aspiration Unit Assy

RBC/Hgb
Chamber SV37 – 38
Reagent Heater WBC Chamber 37
38
38
33
34
Figure 2-9-2-14: RBC Valve Assy

SV33 – 34
Figure 2-9-2-13: Reaction Unit Assy

2.10 HYDRAULIC SEQUENCE DESCRIPTION
0.0 -1.5 sec.

0-1.5 sec.
[Diaphragm Pump Control]

- Vacuum (-0.03MPa) is applied for pneumatic side of diaphragm pumps by activating SV30 for 0.0 – 1.5 seconds.
- EPK diaphragm pump is replenished by activating SV9 for 0.0 – 1.5 seconds.
[RBC/HGB]
- Solution in RBC/HGB Chamber is drained to WC1 by activating SV2 for 0.0 – 1.5 seconds.
[WB Aspiration Probe]
- The Whole blood syringe descends to aspirate 20µL of whole blood sample for 0.2 – 2.0 seconds.

1.5 – 2.5 sec.
1.0mL of EPK is
dispensed into RBC
Chamber
1.5 – 2.5 sec
+0.06 MPa applied

0.6 – 24.0 sec
EPK
+0.06MPa to DPs
1.5 – 2.5 sec
from Rinse Cup

3.4 – 6.4 sec
-0.03 MPa
applied
0.6 – 26.5 sec

1.5 – 2.5 sec..
- Pressure (+0.06MPa) is applied for pneumatic side of diaphragm pumps by activating SV29 for 1.5 – 2.5 seconds.
[RBC/HGB]
- EPK DP dispenses 1.0mL of diluent into RBC/HGB Chamber by activating SV18 for 1.5 – 2.5 seconds.

2.5 – 9.4 sec.

2.5 – 9.4 sec.
[RBC/HGB]
- EPK in RBC/HGB Chamber is charged into Hgb flow cell by activating SV14 for 8.5 – 9.0 seconds.
- Solution in RBC/HGB Chamber is drained by activating SV2 for 9.0 – 9.4 seconds.
[Probe Operation]
- Aspiration probe ascends to the up-most position through the rinse cup for 3.4 to 6.2 seconds.
- EPK is pushed into Rinse cup by activating SV8 and PV-1 while the aspiration probe is ascending through the cup for 3.4 – 6.4 seconds.

9.4 -10.1 sec.

9.4 -10.1 sec.
[RBC/HGB]
- EPK DP dispenses 1.0mL of EPK into RBC/HGB Chamber by activating SV18 for 9.4 – 10.1 seconds.

10.1 – 11.1sec.

10.1 – 11.1sec.
- FFD diaphragm pump is replenished by activating SV28 for 10.1 – 11.1 seconds.
- FFS diaphragm pump replenished by activating SV36 for 10.1 – 11.1 seconds.
[WBC-DIFF]
- Solution in WBC Chamber is drained by activating SV10 for 10.1 – 11.1 seconds.

11.1 – 12.0sec.

11.1 – 12.0sec.
[WBC-DIFF]
- FFD DP dispenses 0.5mL of FFD reagent into WBC Chamber by activating SV27 for 11.1 – 12.0 seconds.
[Sample Aspiration Check]
- Sample Charge Diaphragm Pump is drained by activating SV13 for 11.1 – 12.0 seconds.

12.0 – 14.2sec.

12.0 – 14.2sec.
- Vacuum (-0.03MPa) applied for pneumatic port of diaphragm pumps by activating SV30 for 12.0 – 14.2 seconds.
- FFD diaphragm pump is replenished by activating SV28 for 12.0 – 14.2 seconds.
- 1.0 mL of out of 2mL EPK in RBC chamber is charged into Sample Aspiration Sensor by activating SV1 for “Short Sample” Monitoring, 13.2 – 13.8
seconds. (1.0 mL of EPK remains in RBC Chamber after this operation)

14.2 – 15.5sec.

14.2 – 15.5 sec.
- Pressure “+0.06MPa” is applied for pneumatic side of diaphragm pumps by activating SV29 for 14.2 – 15.5 seconds.

15.5 – 17.0sec.

15.5 – 17.0 sec.
[HGB]
- 1.0 of EPK remained in RBC/HGB Chamber is charged into Hgb flow cell by activating SV14 for 15.5 – 16.9 seconds.
(For blank measurement)
[Probe Operation]
- Sample Aspiration probe at the sample aspirating position moves to above RBC Chamber position driven by STM3 for 15.5 -16.9 seconds.
(At this time, initial position is confirmed beforehand)

17.0 – 18.0sec.

17.0 – 18.0sec.
- Pressure (+0.06MPa) is applied for pneumatic port of diaphragm pumps by activating SV29 for 17.0 – 20.0 seconds.
[Probe Operation]
- Sample aspiration probe descends into RBC/HGB chamber for 17.0 -18.1 seconds.
[RBC/HGB]
- RBC/HGB Chamber is drained by activating SV2 for 17.0 – 18.0 seconds.

18.0 – 20.0sec.

18.0 – 20.0 sec.
- EPK DP dispenses small amount of diluent into RBC/HGB Chamber as the as the primary buffer to prepare whole blood being dispensed by
activating SV18 for 18.0 – 18.1 seconds.
- Sample aspiration probe dispense approx. 2.5µL of whole blood (top end discard portion) into RBC Chamber swinging the probe itself slightly
forward and backword for 19.0 -19.8 seconds.
- Simultaneously, EPK DP dispenses diluent into RBC/HGB chamber for 19.5 – 20.0 seconds. (1mL in total)
At this time, whole blood previously dispensed is mixed with EPK by swirl effect when EPK is injected into RBC chamber.

20.0 – 22.0 sec.

20.0 – 22.0 sec.
- Solution in RBC/HGB Chamber is charged into Blood Aspiration Sensor by activating SV1 for 21.0 - 21.7 seconds. (For Short Sample Check)
then
- Solution remained in RBC/HGB Chamber is drained by activating SV2 for 21.7 – 22.0 seconds.

22.0 – 23.0 sec.

22.0 – 23.0 sec.
- Pressure (+0.06MPa) is applied for pneumatic port of diaphragm pumps by activating SV29 for 22.0 - 23.0 seconds.
- Sample Charge Diaphragm Pump is drained by activating SV13 for 22.0 - 23.0 seconds.
[RBC/HGB]
- 1.0mL of EPK is dispensed into RBC/HGB Chamber by activating SV18 for 22.0 - 23.0 seconds.
[WBC-DIFF]
- To rinse the FCM measurement line and sheath nozzle, following two operations are preformed.
1) FCM sheath flow is formed by activating SV11 for 22.5 - 24.0 seconds;
2) EPK is pushed into FCM measurement line by activation SV3, SV33 for 22.7 - 24.0 seconds, and SV 34 for 22.7 - 23.0 seconds.

23.0 – 24.0 sec.

23.0 – 24.0 sec.
- EPK Diaphragm Pump is replenished by activating SV9 for 23.0 – 25.2 seconds.
- FFD Diaphragm Pump is replenished by activating SV28 for 23.0 – 25.2 seconds.
[RBC/HGB]
[WBC-DIFF]
- FCM Sample Charge line is rinsed by activating SV12 for 23.0 – 24.0 seconds.

24.0 – 25.3 sec.

24.0 – 25.3 sec.
[WBC-DIFF]
- WBC Chamber is drained by activating SV28 for 24.0 – 25.2 seconds.
[Others]
- EPK chamber is replenished by deactivating SV23, activating SV24 from 24.0 seconds and SV15 from 24.5 seconds.
These SV’s (SV15, SV23, SV24,) state are kept until 37 seconds in maximum or 4.0 seconds after EPK Chamber’s Float SW ON, and then turn to
reveres status.

25.3 – 26.6 sec.
EPK Chamber is
Discharging Sample replenished
Charge DP 24.0 – 37.0 sec
4µL of whole blood 25.3 – 26.5 sec
is dispensed into maximum or
RBC Chamber 4.0 sec after
swinging probe itself Float SW ON
-0.03 MPa
slightly applied to EPK
Chamber
24.0 – 37.0 sec
maximum or
4.0 sec after
Float SW ON
+0.06MPa to DP
25.3 – 26.6 sec
To RBC FFD dispensed
Chamber From Sample 25.3 – 26.5 sec
Asp Probe
Sample Charge DP
drained
25.3 – 26.5 sec
From Sample
Charge DP
-0.03 MPa
applied
0.6 – 26.5 sec

25.3 – 26.6 sec.
- Sample Charge Diaphragm Pump is drained by activating SV13 for 25.3 - 26.5 seconds.
[WBC-DIFF]
- FFD DP dispenses 0.5mL of FFD reagent into WBC Chamber by activating SV27 for 25.3 – 26.5 seconds.
[RBC/HGB]
- Aspiration probe in RBC/HGB chamber dispenses 4µL of RBC sample swinging the probe itself slightly forward and backward by activating SV27
for 25.3 – 26.5 seconds.
- 1.0mL of diluent is dispensed into RBC/HGB Chamber by activating SV18 for 25.3 – 26.5 seconds to dilute 4µL of whole blood sample.
(First dispense of EPK for RBC/HGB dilution.)

26.6 – 28.0 sec.

26.6 – 28.0 sec.
- EPK and FFD diaphragm pumps are replenished by activating SV9, SV28, and SV36 for 26.6 - 27.9 seconds.
[WBC-DIFF]
- WBC Chamber is drained two times by activating SV10 for 27.5 - 28.4 seconds, and 27.5 - 28.4 seconds respectively.

EPK Chamber is
28.0 – 30.4 sec. replenished
Sample probe Dispensing 1.0mL 24.0 – 37.0 sec
ascends from RBC of EPK into RBC maximum or
chamber to up-most chamber 4.0 sec after
position swinging Float SW ON
probe itself slightly 28.0 – 29.9 sec
28.7 – 29.8 sec
-0.03 MPa
Sample probe applied to EPK
moves from above Chamber
RBC chamber to 24.0 – 37.0 sec
above WBC maximum or
Chamber 4.0 sec after
29.8 – 30.9 sec Float SW ON
+0.06MPa to DP
FFD dispensed 25.3 – 26.6 sec
28.5 – 29.4 sec
FFS dispensed
29.4 30.3 sec

28.0 – 30.4 sec.
[WBC-DIFF]
- 0.5mL of FFD reagent is dispensed into WBC Chamber by activating SV27 for 28.5 - 29.4 seconds.
- 30µL of FFS reagent is dispensed into WBC Chamber by activating SV35 for 29.4 – 30.3 seconds.
(This operation is not performed in CBC mode.)
[RBC/HGB]
- 1mL of EPK is dispensed into RBC/HGB Chamber additionally by activating SV18 for 28.0 - 29.9 seconds.
At this time, 4µL of whole blood previously dispensed is mixed with 2mL of EPK by the swirl effect when additional EPK is injected into RBC
chamber.
[Probe Operation]
- Sample Aspiration Probe ascends from RBC/HGB chamber to up-most position for 28.7 – 29.8 seconds.
- Sample Aspiration Probe moves backward from RBC/HGB chamber to above WBC chamber position for 28.7 – 29.8 seconds.
[Waste Chamber]
- Waste Chamber -1 is drained by activating SV 19 for 28.5 - 34.1 seconds.

30.4 – 32.9 sec.

30.4 – 32.9 sec.
- Vacuum (-0.03MPa) applied for pneumatic port of diaphragm pumps by activating SV30 for 30.4 - 32.9 seconds.
- EPK, FFD, FFS and SLS reagents are replenished into corresponding diaphragm pumps respectively by activating SV9, SV28, SV32 and SV36 for
30.4 - 32.8 seconds.

32.9 – 34.7 sec.

32.9 – 34.7 sec.
- Sample Charge Diaphragm Pump drained by activating SV13 for 32.9 - 34.8 seconds.
[Probe Operation]
- Sample probe descends into WBC Chamber for 35.2 - 35.3 seconds.
[RBC/HGB]
- Sheath syringe injection path is switched to RBC/PLT measurement line by activating SV37 for 32.7 - 57.3 seconds

34.7 – 37.5 sec.

34.7 – 37.5 sec.
[RBC/HGB]
- RBC sample is charged by activating SV16 and SV38 for 34.7 – 37.4 seconds.
[Probe Operation]
- Aspiration probe descends into WBC chamber for 35.2 –-36.3 seconds.
- Aspiration probe dispense 11µL of whole blood into WBC chamber swinging the probe itself slightly forward and backward for 36.7 – 37.7 seconds.

37.5 – 38.8 sec.

37.5 – 38.8 sec.
[HGB]
- 0.5mL of SLS lyse is dispensed into RBC/HGB chamber by activating SV31 (and SV29) for 37.6 – 38.7. At this time, 1mL of diluted RBC sample
remained in the chamber is mixed and hemolysed with SLS lyse by the swirl effect when SLS is injected into RBC chamber.
(0.5mL of SLS lyse is added into remained 1mL of RBC/HGB diluted sample remained in RBC/HGB chamber.).
[WBC-DIFF]
- 0.5mL of FFD reagent is dispensed into WBC chamber by activating SV27 and SV31 for 37.6 – 38.7 seconds. At this time, 11µL of whole blood
previously dispensed is mixed with 1mL of FFD by the swirl effect when additional FFD is injected into the chamber.
This solution is maintained in the WBC Chamber for about 20 seconds for proceeding reaction.
[RBC]
- RBC Detector is flushed by activating SV4, SV5 and SV7 for 38.3 - 39.1 seconds.

38.8 – 39.8 sec.

38.8 – 39.8 sec.
- FFD and SLS diaphragm pumps are replenished respectively by activating SV28 and SV32 for 38.8 – 39.8 seconds.
[RBC]
- RBC sheath flow is formed by activating SV6 for 39.1 – 58.0 seconds.
- Sheath Syringe ascends for 39.2 – 43.8 seconds at 750pps as RBC pre-drive.
[Probe Operation]
- Sample Aspiration Probe ascends from WBC chamber to upper end position for 38.4 – 39.5 seconds.

39.8 – 41.3 sec.

39.8 – 41.3 sec.
[Probe Operation]
- Sample Aspiration Probe moves forward to above RBC/HGB chamber for 39.8 – 40.9 seconds.

41.3 – 43.4 sec.

41.3 – 43.4 sec.
- SLS diaphragm pump is replenished by activating SV14 for 41.8 – 43.3 seconds.
[Probe Operation]
- Sample Aspiration probe descends into RBC Chamber for 42.8 – 43.7 seconds.

43.4 – 44.1 sec.

43.4 – 44.1 sec.
[RBC]
- Sheath Syringe ascends for 43.9 – 55.7 seconds at 125pps as RBC measurement.

44.1 – 46.8 sec.
Small amount of
Remained whole EPK is dispensed
into RBC Chamber
blood at the top of by activating SV18
probe is dispensed for 0.1 sec
into RBC chamber
with EPK (44.2 – 44.3 sec)
(44.4 – 46.8 sec)
+0.06 MPa to
EPK Chamber is
stopped
44.2 – 55.8 sec
+0.06MPa to DPs
43.4 – 46.8 sec
To RBC Chamber
WB 2.5 µL +
EPK from Asp
Probe Sheath flow path is
(44.4 – 46.8 sec) switched for RBC
measurement
32.7 – 57.3 sec
From RBC
Sample Charge DP Detector
drained
43.4 – 46.8 sec
Sheath Syringe
ascends for
43.9 – 55.7 sec -0.03 MPa
at 125pps applied to WC1
37.0 – 104.8
sec
RBC sheath flow is

formed
39.1 -58.0 sec
WC2 is drained
38.0 – 46.6 seconds in
maximum or 3.0 seconds
after WC2 made empty

44.1 – 46.8 sec.
- Sample Charge Diaphragm Pump drained by activating SV13 for 43.4 – 46.8 seconds.
[RBC]
- Pressure (+0.06MPa) supply to EPK chamber is stopped to keep RBC sheath flow stable, by deactivating SV23 for 44.2 – 55.8 seconds.
Meanwhile, only remained (charged) pneumatic pressure inside the EPK chamber is used to form RBC sheath flow.

- Small amount of EPK is poured into RBC chamber by activating SV18 for 0.1 seconds (44.2 – 44.3 seconds).
This EPK is poured as the buffer liquid for whole blood that dispensed in the next step.
- Remained EPK in EPK Diaphragm Pump is poured into RBC chamber via Sample Aspiration Probe with the whole blood at the tail end discard
portion in the probe by activating SV22 for 44.4 – 46.8 seconds.
By above two operations, approx. 2.5µL of whole blood is diluted with 1.0mL of EPK and this solution is used for “Sample not asp” check in the
following sequence.

46.8 – 49.6 sec.

46.8 – 49.6 sec.
- Solution in RBC Chamber is charged into Sample Aspiration Sensor by activating SV1 for 47.8 – 48.6 seconds.
(For “Sample not Asp” error check)
- Solution in RBC Chamber is drained by activating SV2 for 49.3 – 49.6 seconds.

49.6 – 51.9 sec.

49.6 – 51.9 sec.
[RBC/HGB]
- 1.0mL of EPK is dispensed into RBC Chamber via Aspiration Probe by activating SV22 for 49.6 – 51.9 seconds.
(Rinse Purpose / 1st dispense)

51.9 – 52.7 sec.

51.9 – 52.7 sec.

52.7 – 53.5 sec.

52.7 – 53.5 sec.
[RBC/HGB]
- 1.0mL of EPK is dispensed into RBC Chamber by activating SV18 for 52.7 – 53.5 seconds.
(Rinse Purpose / 2nd dispense)

53.5 – 55.8 sec.

53.5 – 55.8 sec.
[Probe Operation]
- Sample Aspiration Probe ascends from RBC chamber to up-most position for 53.8 – 54.7 seconds.
- Sample Aspiration probe above RBC Chamber moves to above rinse cup for 55.0 – 55.8 seconds.
[RBC/HGB]
- RBC measurement operation finishes at 55.8 seconds.

55.8 – 57.4 sec.

55.8 – 57.4 sec.
- Sample Charge Diaphragm Pump drained by activating SV13 for 55.8 – 57.3 seconds.
[RBC]
- Some amount of EPK in RBC chamber is fed by sheath syringe (returning to home position) into RBC charge line for rinse by activating SV38, 55.8
– 56.3 seconds
- Some amount of EPK is fed into the other side of RBC charge line by activating SV16 for 56.3 – 57.3 seconds.
- RBC Chamber is drained by activating SV2 for 55.7 – 56.2 seconds, and drained again 56.5 – 57.5 seconds.
[WBC-DIFF]
- Pressure (+0.06MPa) is re-applied to EPK Chamber by activating SV23 for 55.8 – 83 seconds.
- Sheath syringe desceds to home position for 55.0 – 57.6 seconds to prepare following DIFF measurement.
- FCM sheath flow is formed by activating SV11 for 55.8 – 81.4 seconds.

57.4 – 58.6 sec.

57.4 – 58.6 sec.
[WBC-DIFF]
- WBC-DIFF sample is charged by activating SV12 and SV34 for 57.4 – 58.6 seconds.

58.6 – 79.3 sec.

58.6 – 79.3 sec.
[WBC-DIFF]
- WBC-DIFF sample at FCM measurement line is injected into FCM Flow Cell by sheath syringe for 58.6 – 60.9 seconds, and by activating SV38 for
58.6 – 79.3 seconds.
While in this period, drive speed of sheath syringe varies as follows;
(1) 58.6 – 60.9 seconds: 1114pps (FCM pre-drive)
(2) 61.0 – 79.3 seconds: 688pps (FCM measurement)

79.3 – 83.0 sec.

79.3 – 83.0 sec.
[Sheath Syringe Reset and Rinse]
- Sheath syringe descends for 9700 pulses, 79.3 – 83.0 seconds. For the space inside the Sheath Syringe descending, EPK is fed by activating
SV3 for 58.6 – 79.3 seconds.
- EPK is pushed into Sheath Syringe by activating SV33 and SV34 for 81.1 – 81.4 seconds.

83.0 – 100.5 sec.

83.0 – 100.5 sec.
From 83.0 seconds, instrument returns to READY status for the next analysis while aftertreatment process is being continued.
When the next anaysis opreration trrigered by pressing [START] button, aftertreatment processs is canceld, then the next analysis sequence will
start.
NOTE:
Aftertreatment proccess includes reset operation of Sheath Syringe position. When aftertreatment is canceled without resetting Sheath
Syringe position, position reset will be performed in the next anaysis sequence while SV33 is activated for 25.5 – 26.1 seconds before the
next measurement process started.

- Sample Charge Diaphragm Pump drained by activating SV13 from 83.0 seconds.
- 1.0mL of EPK is dispensed into RBC Chamber by activating SV18 from 83.0 seconds.
[Others]
- EPK chamber is replenished by deactivating SV23, activating SV24 from 83.0 seconds and SV15 from 83.5 seconds.
These SV’s (SV13, SV18, SV15, SV23, SV24,) state are kept until 100.5 seconds or until 4.0 seconds after EPK Chamber’s Float SW ON, and
then turn to reveres status simultaneously.

100.5-103.2 sec.
EPK DP
replenished
100.5 – 101.5 sec
+0.06 MPa applied to

EPK Chamber
100.5 – 104.8 sec
-0.03MPa to DPs
100.5 – 103.2 sec
WBC-DIFF
charge line
rinsed
102.5 – 103.2
HGB charge line WBC Chamber

rinsed drained
Sheath Syringe 100.5 - 101.5
100.5 – 101.5 sec
and WBC-DIFF
charge line
rinsed
101.5 – 103.2 -0.03 MPa
applied to WC1
37.0 – 104.8
sec
FCM Flow Cell

Rinsed
101.5 - 103.2
Sheath Syringe
returns to home
position
101.5 – 103.4

100.5-103.2 sec.
- EPK Diaphragm pump is replenished by activating SV9 for 100.5 – 101.5 seconds.
[Rinse Operations]
- 1.0mL of EPK in RBC Chamber is charged into Hgb Flow Cell by activating SV14 for rinse purpose, 100.5 – 101.5 seconds.
- EPK is pushed into FCM Flow Cell by activating SV11 for rinse purpose, 101.5 – 103.2 seconds.
- Sheath syringe descends for 5449 pulses, 101.5 – 103.4 seconds. For the space inside the Sheath Syringe descending, EPK is fed by activating
SV3 for 101.5 – 104.1 seconds.
- EPK is pushed into Sheath Syringe and WBC-DIFF measurement line for rinse purpose by activating SV33, 101.5 – 104.1 seconds.
- EPK is pushed into WBC-DIFF sample charge line between WBC Chamber and measurement line for rinse purpose by activating SV34, 102.5 –
103.2 seconds.
- Solution in Rinse Cup line is drained by making PV3 open from 103.2 seconds and this status is kept until all operation completed.

103.2-104.8 sec.

103.2-104.8 sec.
- Sample Charge Diaphragm Pump drained by activating SV13 from for 103.2 – 104.8 seconds.
[Rinse Operations]
- 1.0mL of EPK is dispensed into RBC Chamber by activating SV18, 103.2 – 104.8 seconds.
- Pinch Valve-3 at the Rinse Cup line is opened from 103.2 seconds and kept open until the next analysis triggered to prevent the tube being stuck.
- Pinch Valve-1 and 2 at the Waste Chambers are also opened from 104.2 seconds and kept open until the next analysis triggered to prevent the
tubes being stuck.
- Pressure (+0.06MPa) is applied to drain WC1 by activating SV19 for 104.2 – 104.8 seconds.

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SECTION 2 HYDRAULICS

2.1 PNEUMATIC SYSTEM............................................................ 2

XS-Series S/M December 13, 2007

2.2 USE OF PRESSURE AND VACUUM

XS-Series S/M 2-2 December 13, 2007

Figure 2-3-0-2: Hydraulic System Flow in Capillary Mode

XS-Series S/M 2-3 December 13, 2007

Figure 2-3-1: RBC/HGB Analysis Procedure

XS-Series S/M 2-4 December 13, 2007

2.3.3 Computing the Erythrocyte Indices

XS-Series S/M 2-5 December 13, 2007

Figure 2-3-4: WBC/DIFF Analysis Procedure

XS-Series S/M 2-6 December 13, 2007

Figure 2-3-5: WBC (CBC8) Analysis Procedure

1. A fixed amount of 11µL of whole blood (55.5µL of diluted sample for

XS-Series S/M 2-7 December 13, 2007

Analysis Block RBC/PLT HGB

Table 2-4-2: Analysis Parameters (WBC)

Analysis Block WBC

XS-Series S/M 2-8 December 13, 2007

Figure 2-5 Reaction Chamber Construction

XS-Series S/M 2-9 December 13, 2007

Detector RBC/PLT HGB WBC/DIFF

PLT RBC WBC Histgram DIFF Scattergram

WBC# Lym% BA% MO% NE% EO%

Reagent CELLPACK Ⅱ SULFOLYSER STROMATOLYSER-4DS

XS-Series S/M 2-10 December 13, 2007

PCB No. 2168

The life of the Laser Diode is approx. 10,000 hours.

XS-Series S/M 2-11 December 13, 2007

(4) Front scattered light system

XS-Series S/M 2-12 December 13, 2007

(6) Side fluorescence light system

(7) Side Scattered light system

(8) Cover switch

XS-Series S/M 2-13 December 13, 2007

XS-Series S/M 2-14 December 13, 2007

Figure 2-10-1: 2-Way/3-Way SV Modules Used in XS Hydraulic System

XS-Series S/M 2-15 December 13, 2007

XS-Series S/M 2-16 December 13, 2007

XS-Series S/M 2-17 December 13, 2007

Diluent Chamber Assy Sample Aspiration

Hgb Flow Cell

Figure 2-10-2-2: Front Interior

Figure 2-10-2-1: Left Side Interior

XS-Series S/M 2-18 December 13, 2007

FCM Reaction Unit

RBC Detector Block

Sample Set Unit

Figure 2-9-2-3: Right Top Interior

XS-Series S/M 2-19 December 13, 2007

Figure 2-9-2-6: Hydraulic Unit-C Assy

Figure 2-9-2-5: Hydraulic Unit-B Assy

XS-Series S/M 2-20 December 13, 2007

Figure 2-9-2-8: Valve No. 182 Assy

XS-Series S/M 2-21 December 13, 2007

Air Tank (Vacuum)

Figure 2-9-2-10: Diluent Chamber Assy

XS-Series S/M 2-22 December 13, 2007

Figure 2-9-2-14: RBC Valve Assy

Figure 2-9-2-13: Reaction Unit Assy

XS-Series S/M 2-23 December 13, 2007

XS-Series S/M 2-24 December 13, 2007