Maglumi Manuel Utilisation

MAGLUMI® 600
MAGLUMI® 800
Service Manual
MAGLUMI® 600/800
Immunoassay Analyzers
Snibe Co.,Ltd
IMPORTANT
The information contained herein is based on experience and
knowledge relating to the subject matter as aquired by
Snibe Co.,Ltd.
Snibe is not liable for any loss or damage, including
consequential or special damages resulting from the use and/
or misuse of the contained information, be it negligence or
other fault.
.
Shenzhen New Industries Biomedical
DiaSorin S.p.A. Engineering Co.,Ltd
21st Floor, Block A,Building 1,Shenzhen Software Industry Base,
No.1008, Keyuan Road,Nanshan District,Shenzhen,
518000,P.R.China
www.snibe.com
MAGLUMI® Series Service Manual 2.1 - Revision 01
Snibe Co.,Ltd All rights reserved

Description of Changes
Chapter Change Description
Maglumi® 600/800
Content
Content
Page
1. INTRODUCTION 1-1
1.1 INTENDED USE ............................................................................................................................................. 2
1.2 TYPOGRAPHICAL CONVENTIONS ............................................................................................................... 3
Page
2. INTRODUCTION 2-1
2.1 SYSTEM OVERVIEW .................................................................................................................................... 2
2.1.1 Components .............................................................................................................................................. 2
2.1.2 Internal assemblies.................................................................................................................................... 3
2.1.3 Reaction module process path overview..................................................................................................... 5
2.2 LIQUID CONNECTION .................................................................................................................................. 6
2.2.1 M600 & M800 tubing system .................................................................................................................... 6
2.3 ELECTRIC OVERVIEW ................................................................................................................................. 9
2.3.1 Signal tansmissioin and power supply of the whole analyzer ..................................................................... 9
2.3.2 PCB electronic component ...................................................................................................................... 10
Page
3. INSTALLATION OF THE SYSTEM 3-1
3.1 INTRODUCTION ............................................................................................................................................. 2
3.2 ANALYZER RECEIVED ................................................................................................................................... 2
3.3 UNPACK AND INSTALL THE ANALYZER ........................................................................................................ 2
3.3.1 UNPACK THE ANALYZER ...................................................................................................................... 2
3.3.2 INSTALL THE ANALYZER ...................................................................................................................... 9
3.4 OPEN SERVICE SOFTWARE AND USER SOFTWARE ...................................................................................... 16
3.4.1 Service Software (to adjust the analyzer) ................................................................................... 16
3.4.2 User Software (to check the performance) ................................................................................. 18
Page
4. REMOVAL OFTHESYSTEM 4-1
4.1 INTRODUCTION ........................................................................................................................................... 2
4.2 PREPARATION FOR PACKAGING ................................................................................................................. 2
4.3 PACKAGING ................................................................................................................................................. 2
4.3.1 Check items .............................................................................................................................................. 2
4.3.2 Pack the sensors and cables ...................................................................................................................... 2
4.3.3 Fixing the moving parts ............................................................................................................................ 3
4.3.4 Fixing the analyzer ................................................................................................................................... 5
4.3.5 Packaging the accessories. ........................................................................................................................ 7
Page
5. SERVICE SOFTW ARE 5-1
5.1 INTRODUCTION ........................................................................................................................................... 2
5.2 INCUBATOR.................................................................................................................................................. 2
5.3 WASH LIFT .................................................................................................................................................. 7
5.4 CHAMBER TRANS ...................................................................................................................................... 10
5.5 COMMON FUNCTIONAL PARAMETERS ..................................................................................................... 13
5.5.1 Globals .................................................................................................................................................... 13
5.4.2 Macro ...................................................................................................................................................... 25
Page
6. TROUBLE SHOOTING AND MAITENAN CE 6-1
Service Manual V#2.1 Page 1-1

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Content
6 . 1 Manual of the Cuvette Loader ................................................................................................................. 6
6.1.1 The procedure of dismantling the cuvette loader ...................................................................................... 6
6.1.2 How to replace the components in cuvette loader .................................................................................... 8
6.1.3 Description of M800-05-E04 and M800 05-E05 .................................................................................... 12
6.1.3.1 M800-05-E04....................................................................................................................................... 12
6.1.3.2 M800-05-E05....................................................................................................................................... 12
6.1.4 Problem Shooting ................................................................................................................................... 13
6.1.5 Maintenance............................................................................................................................................ 14
6 . 2 Manual of loader ................................................................................................................................... 15
6.2.1 Sample loader ......................................................................................................................................... 15
6.2.1.1. The procedure of dismantling Sample loader ..................................................................................... 15
6.2.1.2.The replacement and debugging of the component in Sample loader ................................................. 17
6.2.1.2.1 The replacement of the belt in sample loader ................................................................................... 17
6.2.1.2.2 The replacement of electric motor .................................................................................................... 19
6.2.1.2.3 Replace the sample loader device .................................................................................................... 20
6.2.1.3 The possible problem and solution for Sample loader ......................................................................... 21
6.2.1.3.1 Sample loader cannot initialize, move right and left......................................................................... 21
6.2.2 Wash loader ............................................................................................................................................ 23
6.2.2.1 The procedure of dismantling Wash loader ......................................................................................... 23
6.2.2.2.1 The replacement of the belt in wash loader ...................................................................................... 25
6.2.2.2.2 The replacement of electric motor .................................................................................................... 27
6.2.2.2.3 Replace the sample loader device .................................................................................................... 28
6.2.2.3 The possible problem and solution for wash loader............................................................................. 29
6.2.2.3.1 Sample loader cannot initialize, move right and left......................................................................... 29
6 . 3 Manual of Incubator ............................................................................................................................. 31
6.3.1 Overview of the Incubator ...................................................................................................................... 31
6.3.2 Inside of the Incubator ............................................................................................................................ 35
6.3.3 Relevant PCB ......................................................................................................................................... 37
6.3.3.1 Layout of the M600-03-E00 PCB ........................................................................................................ 37
6.3.3.2 Incubator Related Function on the M600-03-E00 PCB ....................................................................... 37
6.3.3.3 Incubator Related Specification on the M600-03-E00 PCB ................................................................ 38
6.3.3.4 Layout of the M800-05-E00-Multiple Module PCB ........................................................................... 39
6.4.3.5 Incubator Related Function on the M800-05-E00 PCB ....................................................................... 39
6.4.3.6 Incubator Related Specification on the M800-05-E00 PCB ................................................................ 39
6.4.3.7 Function Description of the M600-03-E05-Incubator Interface PCB .................................................. 40
6.3.4 Replacement of the Incubator ................................................................................................................. 41
6.3.5 Replacement of Driving Belt .................................................................................................................. 42
6.3.6 Possible Problems and Solutions ............................................................................................................ 43
6.3.6.1 Warning："Incubator regulation front" or “Incubator regulation front” ............................................ 43
6.3.6.2 Warning：“Incubator front move not possible” or “Incubator back move not possible”.................... 44
6.3.6.3 Warning：”Incubator not initialized”.................................................................................................. 45
6.3.6.4 Warning：”Temperature ’Incubator’ is out of range！” ..................................................................... 46
6.3.7 Maintenance............................................................................................................................................ 47
6 . 4 Manual of Washer................................................................................................................................. 48
6.4.1 How to disassemble the Washer component........................................................................................... 48
6.4.2 How to replace a new transport rack....................................................................................................... 50
6.4.3 How to replace a new Washer transport channel? .................................................................................. 51
6.4.4 How to replace the reed and steel beads? ............................................................................................... 52
6.4.5 How to replace the magnets? .................................................................................................................. 53
6.4.6 How to replace the washer lift? .............................................................................................................. 54
6.4.7 How to replace the wash needle? ............................................................................................................ 54
6.4.8 How to replace the back transport push rod? .......................................................................................... 55
6.4.9 How to replace the motor in the Washer area? ....................................................................................... 56
6.4.10 How to replace the location block? ....................................................................................................... 59
6.4.11 Adjustment of Washer lift position in service software ........................................................................ 60
6.4.12 Problem Shooting ................................................................................................................................. 62
6.4.12.1 Washer Loader ................................................................................................................................... 62
6.4.12.2 Wash transport ................................................................................................................................... 63
6.4.12.3 Wash lift ............................................................................................................................................ 63
6.4.12.4 Back transport .................................................................................................................................... 64
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Content
6.4.12.5 Wash needle ....................................................................................................................................... 65
6.4.12.6 Wash soak .......................................................................................................................................... 65
6.4.12.7 Cuvette stuck in Washer Loader ........................................................................................................ 66
6.4.12.8 “Washer peristaltic pump soak abnormally!” ................................................................................... 69
6.4.13 Preventive Maintenance........................................................................................................................ 69
6 . 5 Mannual of Pusher ................................................................................................................................. 70
6.5.1 The procedure of dismantling pusher ..................................................................................................... 70
6.5.2 The replacement of the component in pusher ......................................................................................... 73
6.5.2.1 The replacement of the belt in pusher .................................................................................................. 73
6.5.2.2 Adjustment of pusher belts .................................................................................................................. 78
6.5.2.3 Replacement of electric motor ............................................................................................................. 79
6.5.3 How to adjust the position of the components in pusher ........................................................................ 81
6.5.3.1 The adjustment of height between the pusher and washer ................................................................... 82
6.5.3.2 The adjustment of pusher position. ...................................................................................................... 83
6.5.4 Component 02-Incubator and Pusher...................................................................................................... 85
6.5.4.1 Layout of the 02-E00 PCB................................................................................................................... 85
6.5.4.2 02-E00 PCB Functioning ..................................................................................................................... 85
6.5.4.3 02-E00 PCB electronic component list ................................................................................................ 86
6.5.5 Problem shooting .................................................................................................................................... 86
6.5.5.1 “Pusher not initialized” ........................................................................................................................ 87
6.5.5.2 The wrong position of the pusher movement within specified time .................................................... 88
6.5.6 The maintenance of pusher ..................................................................................................................... 89
6 . 6 Manual of Chamber .............................................................................................................................. 90
6.6.1 How to disassemble the chamber............................................................................................................ 90
6.6.2 How to replace the components in chamber ........................................................................................... 96
6.6.2.1 How to replace the white starter injector ............................................................................................. 96
6.6.2.2 How to replace the chamber glass ....................................................................................................... 96
6.6.2.3 How to replace the chamber transport belt .......................................................................................... 98
6.6.2.4 How to replace the PMT .................................................................................................................... 100
6.6.2.5 How to replace a chamber ................................................................................................................. 101
6.6.3 How to adjust the position of the components in the chamber ............................................................. 103
6.6.3.1 How to adjust the chamber transport ................................................................................................. 103
6.6.3.2 How to adjust the chamber lift ........................................................................................................... 105
6.6.3.3 The definition of blue light’s RLU in range. ..................................................................................... 107
6.6.3.4 The calculation of PMT high voltage. ............................................................................................... 109
6.6.3.5 The measurement of min. value. Cuv ................................................................................................ 109
6.6.4 04-E00 PCB Chamber .......................................................................................................................... 112
6.6.4.1 Function of the 04-E00 PCB .............................................................................................................. 112
6.6.5 Trouble shooting ................................................................................................................................... 113
6.6.5.1 “Chamber transport not initialized” ................................................................................................... 113
6.6.5.2 “Chamber transport move not possible!” ........................................................................................... 115
6.6.5.3 “Chamber lift not initialized” ............................................................................................................ 116
6.6.5.4 “Chamber lift move up/down not possible” ....................................................................................... 117
6.6.5.5 Problem-detecting of the Pumps concerning the chamber ................................................................. 118
6.6.6 The maintenance of chamber ................................................................................................................ 123
6 . 7. Sample&Reagent Area of M600 & Reagent Area of M800 ...................................................... 124
6.7.1 M600 Reagent&Sample Area maintenance .......................................................................................... 124
6.7.1.1 The procedure of dismantling M600 Reagent&Sample Area ............................................................ 124
6.7.1.2 M600-02-E00-Sample&Reagent PCB ............................................................................................... 128
6.7.2 The procedure of dismantling M800 Sample Area ............................................................................... 131
6.7.2.1 The procedure of dismantling M800 Reagent Area ........................................................................... 133
Picture 6.7.17 ................................................................................................................................................. 135
6.7.2.3 M800-05-E00-Multiple Modules (Sample&Reagent Area part) ....................................................... 135
6.7.3 Troubleshooting .................................................................................................................................... 138
6 . 8 Manual of Pipettor module ............................................................................................................. 143
6.8.1 Overview .............................................................................................................................................. 143
6.8.2.How to disassemble the pipettor ........................................................................................................... 145
6.8.2.1 How to disassemble the pipettor(M600) ............................................................................................ 145
6.8.2.2 How to disassemble the pipettor(M800) ............................................................................................ 147
6.8.3 How to replace and adjust the pipettor ................................................................................................. 150

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Content
6.8.3.1 How to replace the pipettor of M600/M800 ..................................................................................... 150
6.8.3.2 How to replace the pipettor needle（M600/800） ............................................................................ 152
6.8.3.3 Disassemble and assemble of the outer belt....................................................................................... 157
6.8.3.4 How to disassemble and assemble the inner arm belt ........................................................................ 162
6.8.3.5 How to replace the vertical rod belt of M600. ................................................................................... 165
6.8.4.M600-02-E00-Sample&Reagent .......................................................................................................... 167
6.8.4.1 Functions description of pipettor’s interface PCBs ........................................................................... 169
6.8.4.2. Functions of cable connectors on M600-02-E01 .............................................................................. 169
6.8.5. Problem shooting ................................................................................................................................. 171
6.8.5.1. Pipettor cannot be coordinated(Mainly for M600 , M800 is similar) ............................................... 171
6.8.5.2. Inner pipetting arm possible problems .............................................................................................. 182
6.8.5.2.1 Inner arm unable to initialize(For M600 only) ............................................................................... 182
6.8.5.2.2 Inner arm unable to move clockwise or anti-clockwise .................................................................. 183
6.8.5.2.3. Outer pipetting arm possible problems(For M600&M800) ........................................................... 184
6.8.5.2.4 Pipetting arm vertical common problems ....................................................................................... 185
6.8.5.2.5 Pipetting possible problems and solutions ...................................................................................... 186
6 . 9 Covers of M600 / M800..................................................................................................................... 189
6.9.1Cover of M600....................................................................................................................................... 189
6.9.1.1 Plate to cover the front ....................................................................................................................... 193
6.9.1.2 Plate to cover the back ....................................................................................................................... 194
6.9.1.3 Plate to cover to top ........................................................................................................................... 195
6.9.1.4 Plates to cover left(8)&right(9).......................................................................................................... 196
6.9.2 Covers of M800 .................................................................................................................................... 198
6.9.2.1 Plate to cover the front ....................................................................................................................... 201
6.9.2.3 Plate to cover the back ....................................................................................................................... 202
6.9.2.4 Plate to cover to top ........................................................................................................................... 204
6.9.2.5 Plates to cover left(8)&right(9).......................................................................................................... 205
Page
7. PUMPS 7-1
7.1.1 DILUTER PUMP ......................................................................................................................................... 2
7.1.1.1 Introduce ................................................................................................................................................ 3
7.1.1.2 How to disassemble and replace the pump ............................................................................................ 4
7.1.1.3 Problem Shooting .................................................................................................................................. 5
7.1.1.4 Volume adjustment of diluter pump ...................................................................................................... 6
7.1.1.5 Maintance of diluter pump .................................................................................................................... 8
7.1.2 STARTER PUMP ......................................................................................................................................... 8
7.1.2.1 Introduce ................................................................................................................................................ 8
7.1.2.2 How to disassemble and replace the pump .......................................................................................... 11
7.1.2.3 Problem Shooting ................................................................................................................................ 11
7.1.2.4 Volume adjustment of starter pump .................................................................................................... 13
7.1.3 VACUUM BYPASS PUMP .......................................................................................................................... 15
7.1.3.1 Introduce .............................................................................................................................................. 15
7.1.3.2 How to disassemble and replace the pump .......................................................................................... 16
7.1.4 CONDENSATE WATER PUMP.................................................................................................................. 18
7.1.4.1 Disassembling of the condensate water pump (Peristaltic pump)........................................................ 18
7.1.4.2 Overview of pump for consendate water (Old design- peristaltic pump) ............................................ 19
7.1.4.3 Structure of the peristaltic pump .......................................................................................................... 20
7.1.4.4 Maintenance to the peristaltic pump .................................................................................................... 20
Page
8. APPENDIX 8-1
8.1 Coordinates M600 ...................................................................................................................................... 3
8.1.1 Preparation for coordinate ........................................................................................................................ 3
8.1.3 Adjustment of reference position ............................................................................................................. 6
8.1.4.1 Adjustment of< First Sampling> position............................................................................................ 10

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8.1.4.2Adjustment of Second Sampling after Incubate ................................................................................... 14
8.1.4.3 Adjustment of Second Sampling after Wash ....................................................................................... 17
8.1.5 Washing position adjust .......................................................................................................................... 22
8.1.6 Sample Reagent area position adjust ...................................................................................................... 24
8.1.6.1 Sample area position adjust ................................................................................................................. 25
8.1.6.2 Reagent area position Adjust ............................................................................................................... 29
8.1.7 Z-Dispense position adjust...................................................................................................................... 36
8.1.8 Adjustment of the pipetting needle in Z- Start position .......................................................................... 40
8.2 Coordinates M800 .................................................................................................................................... 42
8.2.1 Sample Arm/Pipettes .............................................................................................................................. 42
8.2.2 Preparation for coordinate ...................................................................................................................... 43
8.2.3 Procedure of coordinates ........................................................................................................................ 47
8.2.4 Adjustment of reference position ............................................................................................................ 48
8.2.5 Adjustment of pipetting needle ............................................................................................................... 50
8.2.6 Adjustment of <First Sampling> position............................................................................................... 50
8.2.7 Adjustment of Second Sampling after Incubate ..................................................................................... 52
8.2.8 Adjustment of Second Sampling after Wash .......................................................................................... 53
8.2.9 Adjust of the mix position in the second sampling after wash................................................................ 55
8.2.10 Washing position adjust ........................................................................................................................ 56
8.2.12 Reagent area position Adjust ................................................................................................................ 61
8.2.13 Z-diepense position adjust .................................................................................................................... 64
8.2.14 Adjustment of the pipetting needle in Z-start position.......................................................................... 66
8.2.15 Adjustment of data compesation........................................................................................................... 66
8.3 Software Upgrade .................................................................................................................................... 70
8.3.1.1 Kernel or structure of operating software upgrade .............................................................................. 70
8.3.1.2 Operating software patch update ........................................................................................................ 75
8.3.2 Master control PCB Programs’ Upgrade ................................................................................................ 76
8.3.2.1M600-01-E00-COP or M800-01-E00-COPwrite the flash out of the upgrade program ....................... 77
8.3.2.2 M600- 02～04 board or M800-02～05program upgrade writing flash out ......................................... 80

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Content

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Introduction
Chapter 1
Introduction
1. INTRODUCTION.................................................................................................................................................2
1.1 INTENDED USE ................................................................................................................................................2
1.2 TYPOGRAPHICAL CONVENTIONS.....................................................................................................................3
1.2.1 Display of Warnings and Notes ..............................................................................................................3

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Introduction
1. Introduction
The purpose of this document is to provide all technical
instructions to allow a correct installation, maintenance, repair
and uninstallation of the MAGLUMI® Analyzer, including:
MAGLUMI 600 / MAGLUMI 800
This document is intended for Field Service Engineers (FSEs) for

training and performing field service.
1.1 Intended use

The MAGLUMI is a fully automated chemiluminescence analyzer
performing the complete sample processing (sample pre-dilutions,
sample and reagent dispensing, incubations, wash processes,
cuvette transports) as well as the measurement and evaluation.
The instrument is controlled via the Windows PC MAGLUMI®
software.
This software, which was specifically designed for this purpose,

allows the user to process the pre-defined assays.
The System was developed and tested according to the regula
-tions of the IVD Directive. It is intended strictly for professional
In-vitro-Diagnostic use. It is to be used only with Chemilumines-
cence Immunoassays, authorized by SNIBE for the MAGLUMI®
Analyzer.
Any changes to the instrument that are not authorized by the

manufacturer will lead to the loss of the conformity to the
applicable regulations the manufacturer has declared. In this
case, the customer is responsible for the fulfilment of the
applicable regulations.

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Introduction
1.2 Typographical Conventions

The warnings, notes and symbols described hereafter are used
in the current manual, on the instrument and on its packaging.
1.2.1 Display of Warnings and Notes

The signal word "Warning" and a relating symbol point to potential
dangers.
The non-observance of a warning is potential and can result in death or at least
serious irreversible injuries. A damage of the system or an adverse effect on the
system function cannot be excluded.
The signal word "Caution" and a relating symbol point to potential

dangers/problems.
The non-observance of safety instructions can result in minor injuries. A damage
of the system or an adverse effect on the system function cannot be excluded.
The signal word "Caution" point to potential problems.

The non-observance of a safety instruction can result in damage of the system or
an adverse effect on the system function.
The signal word "Note" point to potential problems.

The non-observance of notes can result in an adverse effect on the system function
(result deterioration).
Used Warning Symbols
Caution, risk of danger to person or damage to equipment!

Consult instructions for use!
Biohazard!
Ectrical hazard!

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Introduction
Laser hazard!
Caution, risk of danger to person or damage to equipment!

Consult instructions for use!

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Overview
Chapter 2
Overview
2. OVERVIEW ......................................................................................................................... 2
2.1 SYSTEM OVERVIEW ........................................................................................................... 2
2.1.1 Components ............................................................................................................... 2
2.1.2 Internal assemblies .................................................................................................... 3
2.1.3 Reaction module process path overview ..................................................................... 5
2.2 LIQUID CONNECTION ......................................................................................................... 6
2.2.1 M600 & M800 tubing system .................................................................................. 6
2.3 ELECTRIC OVERVIEW ........................................................................................................ 9
2.3.1 Signal tansmissioin and power supply of the whole analyzer .................................... 9
2.3.2 PCB electronic component ....................................................................................... 11

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Overview
2. Overview
2.1 System Overview

2.1.1 Components
The MAGLUMI® Analyzer is a system consisting of the following components:
 Analyzer
 HP PC-compatible computer with a minimum of 3 serial interfaces
 Keyboard
 Mouse
 Touch-screen monitor
 System operating software (based on Windows application)
 Connecting cable and connecting hoses
 Consumables
 Supply and waste containers

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Overview
2.1.2 Internal assemblies
The internal assemblies of the system are shown as the picture below.
Maglumi600
11
4
13
3 9 15
6 10
2 8
1
5
14
12
1. Pipettors 2. Incubator 3. Sample loader

4. Washer loader 5. Sample/Reagent Area 6. Washer transport
7. RFID reader 8. Back-transport 9. Washer lift
10. Pusher 11. Diluters 12. Barcode reader
13. Measuring chamber 14. Starter Area 15. Waste bag

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Overview
Maglumi800
14
5
2 11 16 17
13
4 9
10
3 8
1 12
15
7
1.Cuvette Loader 2. Sample loader 3. Sample Area

4.Incubator 5. Washer loader 6. RFID reader
7.Barcode reader 8. Pipettor 9. Washer transport
10. Back-transport 11. Washer lift 12. Reagent Area
13. Pusher 14.Dilutor 15. Starter Area
16. Measuring chamber 17.Waste bag
Note:
(1) For the Incubator slots, M600 have ten slots, M800 have 13 slots.
(2) M600 doesn’t include cuvettes Loader
(3) M800 Sample and Reagent Area divide to two individual Area
(4) M600 have 2tracks(16 position) for sample Area, M800 have 5 tracks (40
position)
(5) M600 have 4 tracks for Reagent Area, M800 have 9 tracks for Reagent Area.

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Overview
2.1.3 Reaction module process path overview
Maglumi 600
Wash Meas. Waste

Incu.
Left Pip.
Right Pip.

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Overview
Maglumi800
Waste
Meas.
Wash
Left Pip.
Incu.
Cuv. lo
2.2 Liquid Connection

2.2.1 M600 & M800 tubing system
Note:
(1) M600 and M800 almost have the same tubing system, the different is:
For M600, there is only one condensate tube connect to Sample&Reagent Area,
as the Sample Area and Reagent Area mix together for it.
For M800, there are two condensate tubes connect to Sample Area & Reagent
Area respectively, as Sample Area and Reagent Area separated to two
individual parts.

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Overview

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Overview

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eft
Maglumi® 600/800
Overview
2.3 Electric Overview
2.3.1 Signal tansmissioin and power supply of the whole analyzer
1) Signal transmission
Interface
Board
Upper Main
Computer COP
Board
Sensor
We have 3 dominant ways for signal transmission:

(1) Upper computer→COP→Main board→Interface board→Sensor
(2) Upper computer→COP→Main board→Interface board
(3) Upper computer→COP→Main board→Sensor
2) Power supply network of M600
Maglumi600

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Overview
3) Power supply network of M800

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Overview
2.3.2 PCB electronic component
4 3 1
1. M600-01-E00 PCB board

（1）Control of the temperature in the heating and cooling area.
（2）Liquid level signals of the starters, system liquid and waste liquid
（3）Store the commands of the software, and the components moving control
（1）Control of the vertical, inner and outer motor drivers
（2）Control of the bypass pump
（3）Control of the dilutor
（4）Control of the waste pump of pipettor
（5）Control of the receiving process of liquid level detection signal
（6）Sample and reagent track detection12
（7）Scan the sample track barcode
（8）Read reagent information from RFID
（1）Wash lift control and status
（2）Wash transport control and status
（3）Back transport control and status
（4）Wash pump control
（5）Peristaltic pump control
（6）Washer station overflow detection and warning
（7）Wash station entrance detection
（8）Incubator temperature control and status
（9）Incubator movement control and status

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Overview
（10）Incubator cuvette number scanner
（11）Pusher control and status
（12）Wash load control and status
（13）Sample load control and status
（1）Control of chamber transport
（2）Control of chamber lift
（3）Control of starter pumps
（4）Control of waste pumps
（5）Control of PMT’s high voltage
（6）Control of blue light’s voltage

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Overview
Maglumi800
4 5 1
3 2
1. M800-01-E00 PCB-COP Board

（1）Control of the temperature in the heating and cooling area.
（2）Liquid level signals of the starters, system liquid and waste liquid
（3）Store the commands of the software, and the components moving control
2. M800-02-E00 PCB-Sample Arm Board
（1）Control of the vertical, inner and outer motor drivers
（2）Control of the bypass pump
（3）Control of the dilutor
（4）Control of the waste pump of pipettor
（5）Control of the receiving process of liquid level detection signal
3. M800-03-E00 PCB-Washer Board
（1）Wash lift control and status
（2）Wash transport control and status
（3）Back transport control and status
（4）Wash pump control
（5）Peristaltic pump control
（6）Washer station overflow detection and warning
（7）Wash station entrance detection
4. M800-04-E00 PCB-Chamber Board
（1）Control of chamber transport
（2）Control of chamber lift
（3）Control of starter pumps
（4）Control of waste pumps
（5）Control of PMT’s high voltage

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Overview
5, M800-04-E00 PCB-Multiple Module

(1) Incubator movement control and status
(2) Back transport control and status
(3) Wash load control and status
(4) Sample load control and status
(5) Sample and reagent track detection
(6) Scan the sample track barcode
(7) Read reagent information from RFID
(8) Cuvette loader control and status

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Installation of the System
Chapter 3

3. INSTALLATION OF THE SYSTEM ...................................................................................................................... 2
3.1 INTRODUCTION .............................................................................................................................................. 2
3.2 ANALYZER RECEIVED ................................................................................................................................... 2
3.3 UNPACK AND INSTALL THE ANALYZER ......................................................................................................... 2
3.3.1 UNPACK THE ANALYZER ........................................................................................................................ 2
3.3.2 INSTALL THE ANALYZER ........................................................................................................................ 9
3.4 OPEN SERVICE SOFTWARE AND USER SOFTWARE ....................................................................................... 16
3.4.1 Service Software (to adjust the analyzer) ................................................................................... 16
3.4.2 User Software (to check the performance) ................................................................................. 18

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3. Installation of the System
3.1 Introduction
The package of MAGLUMI series has something different in items. This guideline
can be used for MAGLUMI 600 (M600), MAGLUMI 800 (M800) Chemiluminescence
Analyzers.
3.2 Analyzer Received

Upon receiving the analyzer, please check the condition of the lab: power cable
grounded (very important), room temperature maintained at 15-30℃, and humidity
10% -85%. 220V & 2KV UPS and air conditioner are strongly recommended.
3.3 Unpack and Install the Analyzer

3.3.1 Unpack the analyzer
I. Unpack the box
(1) Drive the screws out of the bottom of the wooden box so that you can lift the rest
of the box up, and the analyzer would stand on the base of the wooden box
(M600, M800).
Unscrew the
base of the
wooden box

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(2) For M600, M800, loosen the fixing screws and move the analyzer to a firm
experiment table or platform.
Fixing screws
(3) Remove the plastic membrane.

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(4) Remove the adhesive tapes and foam plastics on the analyzer.
(5) Unscrew the panel.

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(6) Remove the foam plastics at the washing station.
(7) Remove the cuvettes in the channels.
II. Check items
Check the items in the package strictly for any damages or loss.
For MAGLUMI 600, see Fig. 3.3.1-1 and Fig 3.3.1-2 for reference.
For MAGLUMI 800, see Fig. 3.3.1-3 and Fig 3.3.1-4 for reference.

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Maglumi 600 FULLY AUTO SYSTEM
PACKING LIST
Description Quantity
1 Analyzer 1 口
2 RS-232 cables 2 口
3 DB9 F/F cables 1 口
4 Power cables(3.0M) 1 口
5 Fuse（5.0A、6.0A） 2 口
6 Graduate tube 1 口
7 User Manual 1 口
Notebook Computer 1 口
Battery 1 口
8 Mouse 1 口
Power cable 1 口
AC adapter 1 口
9 Allen Key 1 口
10 Waste liquid bottle （3.3L） 1 口
11 System liquid bottle（3.3L） 2 口
12 Waste container of cuvettes 1 口
13 Cuvette Waste bags 2 口
14 QC Certificate 1 口
15 Cables for download data 1 口
16 jumper terminal connecter 4 口
17 Tray for Sample rack 1 口
18 needle wash tool 1 口
19 Double open end wrench 2 口
20 Disposable syringes（25ml） 1 口
SN:
Checked: Released:
Fig. 3.3.1-1 Maglumi 600 Packing List

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Maglumi 800 FULLY AUTO SYSTEM
PACKING LIST
Description Quantity
1 Analyzer 1 口
2 RS-232 cables 1 口
3 VDE Power cables(1.8M) 3 口
4 Fuse（3.0A、5.0A、6.0A、15.0A） 4 口
5 Graduate tube 1 口
6 User Manual 1 口
7 PC 1 口
8 Keyboard 1 口
9 Mouse 1 口
10 Monitor 1 口
11 Monitor connection cables 1 口
12 Allen Key 1 口
13 Waste liquid bottle （10L） 1 口
14 System liquid bottle（10L） 3 口
15 Caps with sensor and tubing 2 口
16 Waste container of cuvettes 1 口
17 QC Certificate 1 口
18 Cables for download data 1 口
19 Cuvette Waste bags 2 口
20 jumper terminal connecter 4 口
21 Tray for Sample rack 1 口
22 DB9 F/F cables 1 口
23 needle wash tool 1 口
24 conjunction tube 1 口
25 Double open end wrench 2 口
26 digital speaker 1 口
SN:
Checked: Released:

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Fig. 3.3.1-2 Maglumi 600 Package Items

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Fig. 3.3.1-3 Maglumi 800 Package Items

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3.3.2 Install the analyzer
For Maglumi 600 and Maglumi 800 analyzer, place the analyzer on a steady table or
platform. Adjust the four supporting points on the analyzer’s bottom to keep it level.
You need the components below.

(1) Use RS232 to connect computer and analyzer
1. M600
2. M800
RS232 RS232
cannot be
connected
with these
two ports.
RS232
(2) Prepare some system liquid and starters. Get them ready to use.
(3) Connect system liquid tube and sensors

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Note : For M800 only, M600 already connected

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(4). Connect waste tube and sensors
1. M600
2. M800
(4) Connect starters tubes and sensors

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1. For M600
2. For M800

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(5) Connect the Alitea Pump – Pressure latch.

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(6) Fix the waste bag.
Wrong position
Correct position

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3.4 Open Service Software and User Software
3.4.1 Service Software (to adjust the analyzer)
(1) Enter the Service Software (password: snibe923),and click <Initialize> to initialize
the components.
(2) Click <Macro>. Run the “threereactionrunning” to check if the cuvettes would run
smoothly inside the channels.
(3) Click <Coordinates> to align the needle(s) to proper position.
(4) Click <Globals> and then click <Background> to open the “Background” dialog.
Measure the “Background/Closed”, “Background/Open”, and “Background
/Transport Pos.” to check the sealing of the chamber. All the above results should
be less than 100. Measure “Ref. LED on” at last, which has a specified range
140,000-230,000.

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(5) Fully fill the tubing system with system liquid or starters. Click <Globals> and then
<Prime W/C>. Input “45” (or higher) in “Washer” and “20” (or higher) in “Chamber”
to prime the tubes until the tubes of the washing station and the chamber are filled
with system liquid and starters, respectively.
(6) Click <Coordinates> to initialize the pipettor and exit the interface. Click <Wash>
and <Prime> twice to fill the pipettor tubes with system liquid.

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(7) Run “Meas. Cuv.” in the Service Software. Click <Globals>, <Meas. Cuv.>, and
<Start Meas.>. Load a clean and new cuvette on the pusher, and click <OK>.
The specified range “Meas. Cuv.” is 200-1500.
3.4.2 User Software (to check the performance)
(1) Enter the user software (username: snibe, password: snibe).The analyzer will
automatically initialize.

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(2) Make sure there are cuvettes in the left pipetting position and the incubator loader
channel. Run <SystemTest> (Pipettor: 3, Washer:3, Chamber:3, BGW: 1, and
LC:1) in user software. (BGW: 200-1200 CV<5%, LC: 400000-600000, CV<3%).
(3) Calibrate a 1-step assay kit and a 2-step assay kit.
(4) Run internal quality control or external quality control (such as Bio-Rad control) to
check whether the calibration is good or not.

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Removal of the System
Chapter 4
4. REMOVALOFTHESYSTEM...................................................................................... 2
4.1 INTRODUCTION .................................................................................................... 2

4.2 PREPARATION FOR PACKAGING ............................................................................. 2
4.3 PACKAGING ......................................................................................................... 2
4.3.1 Check items ................................................................................................ 2
4.3.2 Pack the sensors and cables ...................................................................... 2
4.3.3 Fixing the moving parts .............................................................................. 3
4.3.4 Fixing the analyzer ..................................................................................... 6
4.3.5 Packaging the accessories. ........................................................................ 9

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4. Removal of the System
4.1 Introduction
The package of MAGLUMI series has something different in items. This guideline
can be used for MAGLUMI® Chemiluminescence Analyzers, including: MAGLUMI
600, MAGLUMI 800, MAGLUMI 1000 (M1000), MAGLUMI 2000 (M2000), MAGLUMI
2000 Plus (M2000P), and MAGLUMI 4000 (M4000).
4.2 Preparation for packaging
I. Finish the maintenance for the analyzer, and ensure that analyzer is in a good
condition.
II. Seal the consumables (system liquid and starters) and put them back to the
fridge.
III. Replace the system liquid and starters with distilled water. Prime the machine
with distilled water 20 times.
IV. Disable the sensors of system liquid and starters with the User Software.
Remove the distilled water. Prime the analyzer with air, making sure there is
no liquid remained in the tubes.
4.3 Packaging
4.3.1 Check items
Please take a look at the packing list in 3.3.1 in Chapter 3. Collect all the items and
put them in one place.
4.3.2 Pack the sensors and cables

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Seal all the sensors and cables separately in plastic bags as the pictures below
incase of contamination.
Picture 4.1
4.3.3 Fixing the moving parts

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I. For the pipetting arm, please take the picture below as an sample.
(1) Fix the arm with solid foam and tie the tubes with belt as the picture below.
Picture 4.2
II. Fix the washer (i.e. washing station), the incubator,and the pusher.
(1) Washer
Lift up the wash lift, and put the three needles into the solid foam plastic
coming along with the analyzer, which has three holes specifically for the
needles.
Picture 4.3

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(2) Incubator
Place three cuvettes in Slot 1 and 2 manually, move the incubator slowly to
match the two channel positions as the picture shows, and then push the
cuvette half inside so that the incubator can be well fixed.
Picture 4.4
(3) Pusher
Place one cuvette inside the pusher, move it to match the back transport
channel, and push half cuvette inside so that the pusher can be well fixed.
Picture 4.5
(4) Doors
Seal the sample area and reagent area doors with tapes.

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Picture 4.6
III. Put all the racks inside the sample area.
Picture 4.7
4.3.4 Fixing the analyzer
I. After the moving parts are fixed, seal the gate with tapes, and.package the
analyzer with plastic membrane. Carried it onto the wooden base, making sure
the four feet are well fitted inside the four hollows on the base.

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Picture 4.8
Picture 4.9
II. Fix the analyzer with chains tying around the lifting handle.
Picture 4.10

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Picture 4.11
III. Seal the analyzer with the wooden box, and glue the lable with arrow pointing
upside.
Picture 4.12

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4.3.5 Packaging the accessories.
Put the accessories and other items back to the box as shown below.
Picture 4.13
Picture 4.14
Picture 4.15

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Tube track
Waste bag
Allen key RS232 line
Tray
System liquid
connecting tube
Picture 4.16
Picture 4.17

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After the accessories are put inside the box, put the solid foam plastics inside and
seal the carton.
This is the final package for the analyzer and the accessories
Picture 4.18

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Service Software
Chapter 5
Service Software
5. SERVICESOFTWARE....................................................................................................................................... 2
5.1 INTRODUCTION .............................................................................................................................................. 2
5.2 INCUBATOR ................................................................................................................................................... 2
5.3 WASH LIFT .................................................................................................................................................... 6
5.4 CHAMBER TRANS .......................................................................................................................................... 9
5.5 COMMON FUNCTIONAL PARAMETERS ......................................................................................................... 12
5.5.1 Globals................................................................................................................................................ 12
5.4.2 Macro.................................................................................................................................................. 24

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Service Software
5. Service Software
5.1 Introduction
In this chapter, some crucial buttons and parameters in the Service Software will be
introduced.
5.2 Incubator
The button is to adjust the positions of the incubator. Please ensure that it is well
aligned; otherwise, the cuvette may get stuck during the tests.
Picture 5.1
Please click <Initialize> before entering the

“Incubator” interface.

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Service Software
Picture 5.2
Adjustment Steps:
Content Steps Note
Step 1 Check the fixed parameters:
Slot count 10
Please write the
Regulation 30
Initialization parameter
Frequency 2000,
firstlbefore
Click <Writeparam> button to
Initialization
save the parameters
Step 2 Click <Initialize> button
Step 1 Put a cuvette in the 1st slot of
the incubator
Select <Initialize> in the “Target
position” , then adjust the
position of <Init>,
Step 2 Click the stepsize bar in “Adjust Remember to
Incubator” to choose the value click the
Initial of the “stepsize” "Writeparam"
position Step 3 button(save the
Click the to adjust the parameters) after
position of the first slot, making adjusting the
the incubator move forward or position
backward until the cuvette can
smoothly get into the washer
from 1st slot
Step 4 Click <Writeparam> button to
save the parameters
Last Step 1 Put a cuvette in the 10th slot of
Position the incubator

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Service Software
Select <Last> in the “Target
position” , then adjust the
position of <Last>,
Step 2 Click the stepsize bar in “Adjust
Incubator” to choose the value
of the “stepsize”
Step 3
Click the to adjust the
position of the 10th slot, making
the incubator move forward or
smoothly get into the washer
from the 10th slot
save the parameters
Step 1 Put a cuvette in the right
pipetting position (second
pipetting position)
Select <Backtr> in the “Target
pos” , then you can adjust the
position of <Backtr>,
Backtr of the “stepsize”
position Step 3
position of the 1st slot, making
smoothly get into the 1st slot
from the pipetting position
save the parameters
Step 1 Put cuvettes from the 1st to 10th
slot position
Select <CuvetScan> in “Target
pos”
of the “stepsize”
CuvetteScan Step 3 M800 does not
Postition position of the 1st slot, making have this option
the reflex sensor above the
incubator exactly over the
middle of the 1st cuvette.
save the parameters.
Step 5 Take the 1st, the 5th, and the
10th cuvette out of the

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Service Software
incubator. Select “All” in “Scan
Incubator” and click <Scan>. If
in the “Has Cuvette(s)” column,
it displays “No” in the 1st, the
5th, and the 10th position and
“Yes” in the others, it means the
adjustment is successful. If not,
repeat Step 2 to Step 4.
Step 1 Click the <Init> button to
initialize the incubator
Step 2 Put a cuvette in the 2nd position
Select <Backtr> in “Target
position”
Enter “2” in the block of “Adjust
of pos” and click <move> button
Then adjust the position of 2nd
slot.
of the “stepsize” For the 2 to 10
Position of slots, you need to
2~10 slots Step 4 adjust the
Click the to adjust the “Backtr” position
position of the 2nd slot, make
smoothly get into the 2nd slot
from the pipetting position

save the parameters
Step 6 Follow the steps above, adjust
the position of the 3 to 10 and
so as for <Backtr> position
Temperature Adjust of the Incubator
Step 1 Put 4 reaction modules into the incubator’s 1st, 5th,
10th slots respectively. Then add 400μl water in the
1th, 3rd, 6th cuvettes of eachreaction module. Turn on
the analyzer, 30 mins is essential to stabilize the
temperature
Step 2 Measure the temperature, if it’s not in the range of
Temperature
36.8±0.5℃,adjust the blue potentiometer on the 03-
Adjustment
E05 PCB on the back panel, 1 circle of the
potentiometer = 0.2℃
If the temperature is higher, adjust it clockwise.
if lower, adjust it counterclockwise
Step 3 Repeat the step 1 and 2 until the temperature is in the
range.

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Service Software
5.3 Wash Lift

The button is to adjust the positions of the wash lift. Please ensure that it is well
aligned; otherwise, the cuvette may get stuck during the tests and the results
may also be affected.
Picture 5.3
Please click <Initialize> before entering the “Wash

Lift” interface.

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Service Software
Picture 5.4
Adjustment Steps ：
Content Steps Note

Click <Writeparam> button to
Step 1 Please write the
save the parameters
parameter firstly
Initialization Click <Initialize> button to make
before
Step 2 sure the washer lift can work
Initialization
normally
Select “Initialize” in the “Target
Position” dialog.
Click the “Stepsize” bar in the
Step 1
“Adjust Incubator” dialogue to
choose the value of the
“Stepsize”.
Move to Init Click to adjust the

Position initialization position of the
washer lift.
Step 2 Observe if the cuvette can go
though the washer channel. The
waste needles must be higher
than the cuvette by 2mm.
Click <Writeparam> to save the
parameters
move to Step 1 Select “Inject” in the “Target

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Service Software
“Inject” Position” dialog.
position Click the “Stepsize” bar in the
“Stepsize”.
Click to adjust the

inject position of the washer lift,
Step 2 observe if the injection needle is
just above the cuvette mouth.
parameters.
Select “Suction” in the “Target
Position” dialog.
Step 1
“Stepsize”.
move to Click to adjust the
suction suction position of the washer
position lift.
Observe if the suction needle
Step 2 just touch the cuvette’s bottom
and make the spring flake of the
needles rise up by 1mm.
parameters
Select “Prime” in the “Target
Position” dialog.
Step 1
“Stepsize”.
move to Click to adjust the
prime prime position of the washer lift.
position Observe if the suction needle
just touch the bottom washer
Step 2 channel’s sink and make the
spring flake of the needles rise
up by 1mm.
parameters.

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Service Software
5.4 Chamber Trans
The button is to adjust the positions of the chamber transport. Please ensure
that it is well aligned; otherwise, the cuvette may get stuck during the tests and
the results may also be affected.
Picture 5.5
Please click <Initialize> before entering the

“Chamber Transport” interface.
Make sure to switch off the analyzer and

disconnect the power supply cable before
opening the cover of chamber.

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Service Software
Picture 5.6
Adjustment Steps ：
Content step remark
Turn off the analyzer, pull out the
connecting cable of PMT and the
reference LED, and open the Switch off the
step1
cover of the chamber. analyzer and
Initialization Turn on the analyzer, run the disconnect the
Service Software. power supply
Click the <Intialize> button in the cable fisrt.
step2 main interface to Initialize the
analyzer.
Click the <Chamb Trans> button
on the main interface to open the
“Chamber Transport” dialog.
Change value of the <Load Pos>
to adjust the angle of the transport
step3 cross, make sure that the transport
Adjustment
wheel is 5-10 degrees clockwise
of the
deviated from the 90° position.
<Load
pos>
parameters. and go back to the
main menu.
Click the <Pusher> button to open
the “Pusher” dialog. Click
step4
“Init/Backtransport” to move the
pusher to this position. Place a

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Service Software
cuvette into the pusher.
Move the pusher to “Chamber”
position. Observe if the gap of
cuvette’s bottom can fit the
transport wheel smoothly.
If not ,readjust the value of the
<Load Pos>.
Click the <Globals> button and
then the <Meas.cuv> button to
step6 open the “Measure cuv” dialog.
Run a test as the Page5-16
instructed.
Observe process of the “Measure.
Cuv” carefully in the chamber.
Adjustment
step7 Check if the injector of the starters
of the
can align with center of the cuvette
<Adjust
cavity.
pos>
If not, we can adjust the “Adjust
Pos” in the “Chamber Transport”
dialog to make the injector can
step8 align with cuvette’s vial.
(We can also achieve that purpose
by adjust screw on the injector
manually)
After finishing all the settings, Click
<Writeparam> to save the
parameters.
Put a cuvette in the pusher, move
the pusher to the “Chamber”
step9
position. In the “Chamber
Transport” dialog, input 14 in the
“Move count”, and click the
<Move> button to check if the
cuvettte can move smoothly

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Service Software
5.5 Common Functional Parameters
5.5.1 Globals
When clicking <Globals>, you will see an interface divided into two parts: function
buttons (blue circled), and some basic parameters (red circled).
Picture 5.7
Only when the corresponding component is

normal, and has been initialized, can these
buttons work normally.
I. <Prime W/C>
It is to prime the washer or the chamber. You can change the times.
Picture 5.8
Adjustment Steps：
Content Steps Note
Washer Step Click <Initialization> in the main Please ensure

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Service Software
Washing 1 menu to initialize the analyzer that the
Step Click the <Globals>—<Prime W/C> “Initialization”
2 to enter the “Prime Washer/Chamber” has been done
dialog at first
Step Input the washing Cnt，and then click
3 the <Washer> button.
Step Check if the 3 washing pumps and
4 Washer peristaltic pump are working
normally.
Step Click <Initialization> in the main
1 menu to initialize the analyzer
Step Click the <Globals>—<Prime W/C>
2 to enter the “Prime Washer/Chamber”
Chamber dialog
Washing Step Input the chamber Cnt，and then click
3 the <Chamber> button.
Step Check if the 2 Starter reagent pumps
4 and chamber-soak pump are working
normally.
II. <Background>
Background check should be performed in the first place to ensure there is no light
leakage in the chamber and the reference LED is at proper state.
Picture 5.9
Adjustment Steps：
Content Steps Note
Dark Count Step 1 Click <Initialization> in the main 1. Please
measuremen menu to initialize the analyzer. ensure that

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Service Software
t under Step 2 Click the <Globals>— the
different <background> to open the “initialization”
models “backgroung” dialog. have been
Step 3 Input the “Meas.time” and done in the
“Meas.cycles” (Use default value first place
commonly). 2. Do not open
Choose the check box among the the cover of
four kinds of measurement. the chamber
Then press <Start.meas>. or disconnect
Step 4 1.”Background/Closed” <100 the cable of
”Background/Open” <100 the PMT
”Background/Transportpos” <100
If either of the above 3 is out of
range, please check the sealing of
the chamber.
2. The value of the “Ref. LED on” is
supposed to be between 140,000-
230,000, This value can be adjusted
by changing the value of the “D/A
Ref Led” on the “Globals” interface.
If it’s bigger, the value of “Ref. LED
on” in “Background” will be higher.
III. <Plateau> (PMT’s Voltage)

Plateau is to decide the high voltage for PMT and to check the performance of PMT.
It will work combined with the Reference LED; therefore, please make sure the value
of “Ref. LED on” in “Background” is between 140,000-230,000 in the first place.
Click the <Plateau> button to open the “Plateau” dialog; you will see the figure below.
Picture 5.10

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Service Software
Parameter Description：
Meas. time: each measuring time of the PMT, the default value is 1s.
Step With: each transition range of the PMT high voltage’s measurement. The
default value is 5.
Average count: the measuring number when the PMT measure the RLUs on PMT’s
high-voltage. The default value is 3
Max RLU: the max RLU of the Y-axis
Scal: the RLU of the Y-axis’s min graduation
Adjustment Steps：
Content Steps Note
Step 1 Click the <Globals>—<Plateau> to
open the “Plateau” dialog.
Step 2 Input “300000” in “Max RLU”,
choose “50000” in “Scal.”, and click
the <Draw diagr.> button. Choose 1. Please
the check box “Reference LED on”. ensure that
Step 3 Click the <Start> button to draw the the
D/A value curve. It should be smooth “initialization”
as shown in the figure below. If not, have been
please adjust the value of the "D/A done in the
Ref Led" and repeat Step 2 to Step first place
PMT 3. 2. Choose the
Voltage’s Step 4 After getting the smooth curve, take right value of
setting down the “Suggest D/A highvolt”. the “D/A Ref
Click the <Start> button for another Led” to make
4 times to record the “Suggest D/A sure the value
highvolt”. The difference between of the “Ref.
these 5 values should be less than LED on” is
or equal to 5. If not, please adjust between
the value of the “D/A Ref Led” and 140,000 and
repeat Step 2 to Step 4. 230,000
Step 5 When we get all the values of
“Suggest D/A highvolt”. Input the
smallest one in “D/A highvolt” in the
“Global” interface.

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Service Software
Picture 5.11
IV. < Meas. Cuv. >
It is to test the background of the Starters and to check the performance of the whole
chamber. When you perform it alongside with BGW in the User Software, you can
rule out whether the problem is about the washer or the chamber.
Picture 5.12
Adjustment Steps：
Content Step Note
Check the Step 1 Click <Initialization> in the main 1. Please ensure

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Service Software
“Meas.cuv” menu to initialize the analyzer. that the
Step 2 Press <Globals>--<Meas.cuv> to “initialization”
open the “Meas.cuv” dialog. have been done
Step 3 Click the <Start.Meas> button， at first place
then the software will show that 2. Do not open
“please insert cuvette in pusher”. the cover of the
Step 4 Put the cuvette in the pusher, click chamber or
the <OK> button, and the analyzer disconnect the
will begin to measure the values. cable of the
Step 5 Repeat the step 3 to step 4 for 3 PMT
times.
Result : 500-1500, CV < 5%
V. <Errorcard>
M600 Errorcard Interface

Picture 5.13

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Service Software
M800 Errorcard Interface

Picture 5.14
The red circled area can be changed. Use a multimeter to measure the voltage of the
positions shown below and input the values measured in the corresponding blanks.
As to the temperature, please measure with a thermometer at the corresponding
positions and input the values in the blanks.
(1) For M600 disconnect the cable between the switching power supply and M600-
01-E01 board, Sample & Reagent Area when the machine is powered off. Switch
on the main switch of the analyzer, and then check the +24V output of the power
supply.

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Service Software
DC voltage output of the switching power supply

Picture 5.15
Measure the +15V, -15V, and +5V voltage with the black end touching the
ground and the red end to the corresponding measuring holes on M600-04-E00
PCB.
Measuring holes on the M600-04-E00 PCB

Picture 5.16
(2) For M800 disconnect the cable between the switching power supply and M800-
01-E01 board, Sample & Reagent Area when the machine is powered off. Switch
on the main switch of the analyzer, and then check the +24V output of the power
supply.

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Service Software
DC voltage output of the switching power supply

Picture 5.17
Measure the +5V and -5V voltage with the black end touching the ground and the
red end to the corresponding measuring holes on the M600-04-E00. Measure the
+48V voltage the black end touching the ground and the red end to the
corresponding measuring hole on the M800-05-E00.

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Picture 5.18
(3) Measure the temperature and save parameters
When the analyzer is turned on ,the temperature of different components will be

stable after 1 hour. Measure the temperature and click <Calc.Temp>. Remember
to save the parameters by clicking <Writeparam>.
VI. <System Test>
This is a newly added button in our latest software (ver: 1.14.8.8). It is to set the
accepted range for BGW and Light Check (LC). If their values exceed this range,
they will be marked red in the User Software.
For BGW, the recommended value range is 200 to 1200, with the CV less than 10%.
Please note that 10% is only for BGW around 200. If the BGW is over 500, it is better
that the CV is less than 5%.
For LC, the recommended value range is 400,000 to 650,000, with the CV less than
3%.
You can set your own range if the above does not meet your needs.
Picture 5.19

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Service Software
VII. Min Value Cuv
The function of “Min value cuv.” is to test whether there is cuvette is in the chamber
or not. Once the value generated by the PMT is higher than this number, the machine
will determine that no cuvette is in the chamber.
Picture 5.20
Adjustment Steps：
Content Steps Note
Step 1 Click <Initialization> in the main
menu to initialize the analyzer.
Step 2 Press <Globals>--<Background>
Measure the “Ref. LED on” value
for 5 times and record the average 1. Make sure
value X1. that the value of
Step 3 Move the pusher to the the "D/A Ref
“backtransport position”, and put a Led" has been
cuvette in pusher. Move the well adjusted
Set
pusher to the “chamber position”. 2. When moving
parameter
Step 4 Move the chamber transport 8 the pusher or
of the
steps to make the cuvette one chamber
“Min.value.
cavity ahead the PMT in the transport,
cuv”
chamber. please use the
Step 5 Press <Globals>-- corresponding
<Background>. buttons in the
Measure the “Ref. LED on” value Service
for 5 times and record the average Software.
value X2.
Step 6 Min Value Cuv. = (X1 + X2) / 2
Then fill it in the "Min Value Cuv."
and click <Writeparam>.

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VIII. RLU Factor
Since different PMTs cannot perform exactly the same, we use RLU Factor to bring
about similar RLUs on various PMTs. The formula of RLU is as below.
Sample/QC/Calibrator signal - BGW signal
RLU   RLU Factor
1000
Picture 5.21
When the CV of Light Check (LC) is within 3%, and the value is not in range (i.e.
lower than 400,000 or higher than 600,000), you can adjust RLU Factor in the service
software using the formula below.
Original RLU factor × Expected LC result

New RLU factor 
Average of measured LC results of both needles
For machines with only one pipettor, the formula is:
Original RLU factor × Expected LC result

New RLU factor 
Measured LC result
Adjustment Steps：
Content Step Note
Step 1 Perform LC in the User Software.
Step 2 Check the difference between the
1. Please make
LC results of the two needles. If it
Set the sure the LC
is larger than 5%, please adjust
proper difference
the volume of the pipetting
RLU between left and
needles first. (For machines with
Factor right needle is
two pipettors only)
within 5% first
Step 3 Choose 500,000 (recommended)
as the expected LC result and

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calculate the RLU Factor with the
above mentioned formula.
Step 4 Input the result in “RLU Factor”
and click <Writeparam>.
5.4.2 Macro
The <Macro> button is mainly used to check the alignment of various parts except
the needle. After click <Select> on the “Macro Interpreter” interface, we oftern
choose “threereactionrunning. cmd” to move three reaction modules in the channels
of different components in order to see if they can move smoothly. Normally we
recommend running this at least 15 cycles, which means 45 reaction modules will be
used.
Picture 5.22

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Trouble Shooting and Maintenance
Chapter 6
TROUBLE SHOOTING AND
MAITENANCE
6.1 Manual of the Cuvette Loader ................................................................. 6
6.1.1 The procedure of dismantling the cuvette loader ............................. 6
6.1.2 How to replace the components in cuvette loader ........................... 8
6.1.2.1 The replacement of M800-05-E05 PCB .................................... 8
6.1.2.2 The replacement of M800-05-E04 PCB .................................... 9
6.1.2.3 The replacement of cuvette loader motor and (or) belt............ 10
6.1.3 Description of M800-05-E04 and M800 05-E05 ............................. 12
6.1.3.1 M800-05-E04 .......................................................................... 12
6.1.3.2 M800-05-E05 .......................................................................... 12
6.1.4 Problem Shooting .......................................................................... 13
6.1.4.1 Cuvette loader cannot normally load reaction modules ........... 13
6.1.4.2 “Running out of cuvettes soon” ............................................... 13
6.1.4.3 Warning that reaction module is used up ................................ 14
6.1.5 Maintenance .................................................................................. 14
6.2 Manual of loader .................................................................................... 15
6.2.1 Sample loader ................................................................................ 15
6.2.1.1. The procedure of dismantling Sample loader ......................... 15
6.2.1.2.The replacement and debugging of the component in Sample
loader .................................................................................................. 17
6.2.1.2.1 The replacement of the belt in sample loader ...................... 17
6.2.1.2.2 The replacement of electric motor ........................................ 19
6.2.1.2.3 Replace the sample loader device ..................................... 19
6.2.1.3 The possible problem and solution for Sample loader............. 20
6.2.1.3.1 Sample loader cannot initialize, move right and left ............. 20
6.2.2 Wash loader ................................................................................... 22
6.2.2.1 The procedure of dismantling Wash loader ............................. 22
6.2.2.2 The replacement and debugging of the component in Sample
loader .................................................................................................. 24
Service manual V#2.1 Page6-1

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Trouble Shooting and maintence
6.2.2.2.1 The replacement of the belt in wash loader .......................... 24
6.2.2.2.2 The replacement of electric motor ........................................ 26
6.2.2.2.3 Replace the sample loader device ..................................... 27
6.2.2.3 The possible problem and solution for wash loader ................ 28
6.2.2.3.1 Sample loader cannot initialize, move right and left ............. 28
6.3 Manual of Incubator ............................................................................... 30
6.3.1 Overview of the Incubator .............................................................. 30
6.3.2 Inside of the Incubator ................................................................... 33
6.3.3 Relevant PCB ................................................................................ 35
6.3.3.1 Layout of the M600-03-E00 PCB ............................................ 35
6.3.3.2 Incubator Related Function on the M600-03-E00 PCB ........... 35
6.3.3.3 Incubator Related Specification on the M600-03-E00 PCB ..... 36
6.3.3.4 Layout of the M800-05-E00-Multiple Module PCB .................. 37
6.4.3.5 Incubator Related Function on the M800-05-E00 PCB ........... 37
6.4.3.6 Incubator Related Specification on the M800-05-E00 PCB ..... 37
6.4.3.7 Function Description of the M600-03-E05-Incubator Interface
PCB ..................................................................................................... 38
6.3.4 Replacement of the Incubator ........................................................ 39
6.3.5 Replacement of Driving Belt .......................................................... 40
6.3.6 Possible Problems and Solutions .................................................. 41
6.3.6.1 Warning："Incubator regulation front" or “Incubator regulation
front” .................................................................................................... 41
6.3.6.2 Warning：“Incubator front move not possible” or “Incubator
back move not possible” ..................................................................... 42
6.3.6.3 Warning：”Incubator not initialized” ........................................ 43
6.3.6.4 Warning：”Temperature ’Incubator’ is out of range！” ............ 44
6.3.7 Maintenance .................................................................................. 45
6.4 Manual of Washer .................................................................................. 46
6.4.1 How to disassemble the Washer component ................................. 46
6.4.2 How to replace a new transport rack ............................................. 47
6.4.3 How to replace a new Washer transport channel?......................... 49
6.4.4 How to replace the reed and steel beads?..................................... 50
6.4.5 How to replace the magnets? ........................................................ 51
6.4.6 How to replace the washer lift? ...................................................... 52
6.4.7 How to replace the wash needle? .................................................. 52
6.4.8 How to replace the back transport push rod? ................................ 53
6.4.9 How to replace the motor in the Washer area? .............................. 54
6.4.10 How to replace the location block? .............................................. 56
6.4.11 Adjustment of Washer lift position in service software .................. 57
6.4.12 Problem Shooting ........................................................................ 59
Page 6-2 Service manual V#2.1

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6.4.12.1 Washer Loader ...................................................................... 59
6.4.12.2 Wash transport ...................................................................... 60
6.4.12.3 Wash lift ................................................................................ 60
6.4.12.4 Back transport ....................................................................... 61
6.4.12.5 Wash needle ......................................................................... 62
6.4.12.6 Wash soak ............................................................................ 62
6.4.12.7 Cuvette stuck in Washer Loader ........................................... 63
6.4.12.8 “Washer peristaltic pump soak abnormally!” ....................... 66
6.4.13 Preventive Maintenance .............................................................. 66
6.5 Mannual of Pusher ................................................................................. 68
6.5.1 The procedure of dismantling pusher............................................. 68
6.5.2 The replacement of the component in pusher ................................ 70
6.5.2.1 The replacement of the belt in pusher ..................................... 70
6.5.2.2 Adjustment of pusher belts ...................................................... 75
6.5.2.3 Replacement of electric motor ................................................. 76
6.5.3 How to adjust the position of the components in pusher ................ 79
6.5.3.1 The adjustment of height between the pusher and washer ..... 79
6.5.3.2 The adjustment of pusher position. ......................................... 80
6.5.4 Component 02-Incubator and Pusher ............................................ 82
6.5.4.1 Layout of the 02-E00 PCB....................................................... 82
6.5.4.2 02-E00 PCB Functioning ......................................................... 82
6.5.4.3 02-E00 PCB electronic component list .................................... 83
6.5.5 Problem shooting ........................................................................... 83
6.5.5.1 “Pusher not initialized” ............................................................. 84
6.5.5.2 The wrong position of the pusher movement within specified
time ..................................................................................................... 85
6.5.6 The maintenance of pusher ........................................................... 86
6.6 Manual of Chamber................................................................................ 87
6.6.1 How to disassemble the chamber .................................................. 87
6.6.2 How to replace the components in chamber .................................. 92
6.6.2.1 How to replace the white starter injector ................................. 92
6.6.2.2 How to replace the chamber glass .......................................... 92
6.6.2.3 How to replace the chamber transport belt.............................. 94
6.6.2.4 How to replace the PMT .......................................................... 96
6.6.2.5 How to replace a chamber ...................................................... 97
6.6.3 How to adjust the position of the components in the chamber ....... 99
6.6.3.1 How to adjust the chamber transport....................................... 99
6.6.3.2 How to adjust the chamber lift ............................................... 101
6.6.3.3 The definition of blue light’s RLU in range. ............................ 103
6.6.3.4 The calculation of PMT high voltage. .................................... 105

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6.6.3.5 The measurement of min. value. Cuv.................................... 105
6.6.4 04-E00 PCB Chamber ................................................................. 107
6.6.4.1 Function of the 04-E00 PCB .................................................. 107
6.6.5 Trouble shooting .......................................................................... 108
6.6.5.1 “Chamber transport not initialized” ........................................ 108
6.6.5.2 “Chamber transport move not possible!” ............................... 110
6.6.5.3 “Chamber lift not initialized” ................................................... 111
6.6.5.4 “Chamber lift move up/down not possible” ............................ 112
6.6.5.5 Problem-detecting of the Pumps concerning the chamber .... 113
6.6.6 The maintenance of chamber ...................................................... 118
6.7. Sample&Reagent Area of M600 & Reagent Area of M800................ 119
6.7.1 M600 Reagent&Sample Area maintenance ................................. 119
6.7.1.1 The procedure of dismantling M600 Reagent&Sample Area 119
6.7.1.2 M600-02-E00-Sample&Reagent PCB ................................... 123
6.7.2 The procedure of dismantling M800 Sample Area ....................... 126
6.7.2.1 The procedure of dismantling M800 Reagent Area ............... 128
6.7.2.3 M800-05-E00-Multiple Modules (Sample&Reagent Area part)
.......................................................................................................... 130
6.7.3 Troubleshooting ........................................................................... 132
6.8 Manual of Pipettor module .................................................................. 138
6.8.1 Overview ...................................................................................... 138
6.8.2.How to disassemble the pipettor .................................................. 139
6.8.2.1 How to disassemble the pipettor(M600) ................................ 139
6.8.2.2 How to disassemble the pipettor(M800) ................................ 142
6.8.3 How to replace and adjust the pipettor ........................................ 145
6.8.3.1 How to replace the pipettor of M600/M800 ......................... 145
6.8.3.2 How to replace the pipettor needle（M600/800） ................. 147
6.8.3.3 Disassemble and assemble of the outer belt......................... 151
6.8.3.4 How to disassemble and assemble the inner arm belt .......... 156
6.8.3.5 How to replace the vertical rod belt of M600. ........................ 159
6.8.4.M600-02-E00-Sample&Reagent .................................................. 161
6.8.4.1 Functions description of pipettor’s interface PCBs ................ 162
<1> M600-02-E01 PCB function description ..................................... 162
<4>M600-02-E04 PCB function description ...................................... 163
6.8.4.2. Functions of cable connectors on M600-02-E01 .................. 163
6.8.5. Problem shooting ........................................................................ 164
6.8.5.1. Pipettor cannot be coordinated(Mainly for M600 , M800 is
similar)............................................................................................... 164

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6.8.5.2. Inner pipetting arm possible problems.................................. 175
6.8.5.2.1 Inner arm unable to initialize(For M600 only) ..................... 175
6.8.5.2.2 Inner arm unable to move clockwise or anti-clockwise ....... 176
6.8.5.2.3. Outer pipetting arm possible problems(For M600&M800) . 176
6.8.5.2.4 Pipetting arm vertical common problems ........................... 178
6.8.5.2.5 Pipetting possible problems and solutions ......................... 179
6.9 Covers of M600/M800 .......................................................................... 182
6.9.1Cover of M600 .............................................................................. 182
6.9.1.1 Plate to cover the front .......................................................... 186
6.9.1.2 Plate to cover the back .......................................................... 187
6.9.1.3 Plate to cover to top .............................................................. 188
6.9.1.4 Plates to cover left(8)&right(9) ............................................... 189
6.9.2 Covers of M800 ........................................................................... 190
6.9.2.1 Plate to cover the front .......................................................... 193
6.9.2.3 Plate to cover the back .......................................................... 194
6.9.2.4 Plate to cover to top .............................................................. 196
6.9.2.5 Plates to cover left(8)&right(9) ............................................... 197

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6.1 Manual of the Cuvette Loader

This part introduces the disassembling and assembling of the cuvette loader
and some other components in cuvette loader along with the problem shooting
of the cuvette loader.
Picture 6.1.1
6.1.1 The procedure of dismantling the cuvette loader

(1) Take off the cover of the analyzer.
(2) Unscrew three screws of the incubator loader.
Picture 6.1.2
(3) Unscrew the two screws of the incubator loader pad.

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Picture 6.1.3
(4) Unscrew the three screws of the cuvette loader.

(5) Unplug the cable circled in the picture below and take out the cuvette
loader.

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Picture 6.1.4
6.1.2 How to replace the components in cuvette loader
6.1.2.1 The replacement of M800-05-E05 PCB
(1) Follow 6.1.1 to take out the cuvette loader.

(2) Unscrew the three screws on the right side of the cuvette loader.
Picture 6.1.5
Note: There are three plastic O rings on the other side of the PCB. Be
aware when unscrewing the three screws of the PCB in case losing them.

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Picture 6.1.6
(3) Replace it with another PCB if it is broken.

(4) Fix the screws and parts back to the analyzer.
6.1.2.2 The replacement of M800-05-E04 PCB

(2) Unscrew the three screws on the left side of the cuvette loader.
Note: There are three plastic O rings on the other side of the PCB. Be
aware when unscrewing the three screws of the PCB in case losing them.
Picture 6.1.7
(3) Disconnect the cables of the PCB with the loader, replace another one and
weld the cables.
Note: Mind the color of the cables and do not mix them up.

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Picture 6.1.8
6.1.2.3 The replacement of cuvette loader motor and (or) belt

(2) Unscrew the two screws of cuvette entrance device.
Picture 6.1.9
(3) Unscrew the two screws connected to the motor.

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Picture 6.1.10
(4)Take off the motor to replace the motor or belt(s).


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6.1.3 Description of M800-05-E04 and M800 05-E05
6.1.3.1 M800-05-E04
Picture 6.1.11
There are three sensors in the cuvette loader: Cuvette Loader Sensor,
Cuvete-empty Sensor, Cuvette-out Sensor.
On the M800-05-E04 PCB, there is one emitter of Cuvette Loader Sensor, two
receivers of Cuvette-empty Sensor (U4) and Cuvette-out Sensor (U1) .
Three potentionmeters (W1, W2, W3) are used to adjust the sensitivity of
Cuvette Loader Sensor, Cuvete-empty Sensor and Cuvette-out Sensor
respectively.
6.1.3.2 M800-05-E05
There are two emitters (D1, D2) for Cuvete-empty Sensor and Cuvette-out
Sensor.

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6.1.4 Problem Shooting
6.1.4.1 Cuvette loader cannot normally load reaction modules
Phenomenon:
When loading reaction modules, cuvette loader cannot recognize them and
thus the belt does not move.
Possible reasons:
(1) Cuvette Loader Sensor is not well adjusted.
(2) Cuvette Loader Sensor is broken.
Solutions:
(1) Manually block the emitter of the Cuvette Loader Sensor with a reaction
module and observe the change of the D1 signal light. D1 shall be on when
blocked and off when not blocked. If it dese not change accordingly, adjust
W1.
(2) After adjustment of W1, if D1 still does not change, chances are that the
sensor or the emitter is broken. Replace the sensor or the emitter.
6.1.4.2 “Running out of cuvettes soon”
Phenomenon:
There are exactly 6 or more than 6 reaction modules in the loader but the
analyzer alarms “Running out of cuvettes soon”.
Possible reasons:
(1) Cuvette-empty Sensor is not well adjusted.
(2) Cuvette-empty Sensor is broken.
Solutions:
(1) Manually block the emitter of the Cuvette-empty Sensor with a reaction
W2.

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6.1.4.3 Warning that reaction module is used up
Phenomenon:
There are reaction modules in the loader but the analyzer alarms that reaction
module is used up.
Possible reasons:
(1) Cuvette-out Sensor is not well adjusted.
(2) Cuvette- out Sensor is broken.
Solutions:
(1) Manually block the emitter of the Cuvette- out Sensor with a reaction
W3.
6.1.5 Maintenance
(1) Replace the belts of cuvette loader every year.

(2) Clean the surface of the cuvette loader every week.

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6.2 Manual of loader

6.2.1 Sample loader
6.2.1.1. The procedure of dismantling Sample loader
Please follow these steps below!
(Please keep the analyzer power off during these processes!)
Step 1: Please take the white panel out of the sample loader (as showed in the
red ellipse of picture 6.2.1B);
This one is the

sample loader
B C
Take this panel

out of socket
Picture 6.2.1

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Step 2: Screw these two screws in red ellipse of picture 6.2.2A; Then two new
screws will be shown in front of us, and screw other 3 screws in the red ellipse
of picture 6.2.2B to dismantle the sample loader.
A B
Picture 6.2.2
Step 3: Finally, three supporting rods will appear in front of us (as showed in
Picture 6.2.3A), and we can take sample loader out of the analyzer (depicted in
picture 6.2.3B).
This is the end of how to dismantle the pusher from the analyzer.
A B
Sample loader
Three rods
Picture 6.2.3

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6.2.1.2.The replacement and debugging of the component in
Sample loader
6.2.1.2.1 The replacement of the belt in sample loader
Step 1: Just loosen a little of two screws showed in Picture 6.2.4A, and then
the pressure of the belt will become weak （Showed in Picture 6.2.4B） and
you can take the belt out of the motor gear( Showed in picture 6.2.4C ).
A B
Rotate the belt to

take it off the motor
C gear
Picture 6.2.4
Step 2: Screw the two screws in the red ellipse as showed in Picture6.2.5A,
and then you can take the belt out from sample loader (Picture 6.2.5B).

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A B
Picture 6.2.5
Step 3: When replacing a new belt, you can follow the procedure (from picture
6.2.5 to picture 6.2.4) and finish the procedure of replacement. Moreover, after
finishing the procedure of replacing belt, you may find the belt may loosen a
little than before, and you can push screws (showed in red ellipse in picture
6.2.6A ) up to make the belt tight like the previous situation and then fix screws.
After finish this procedure, the two screws in Picture 6.2.6 B can be fixed too.
A B
Fix these two screws

after finish procedure
Pushes up the two in picture A
screws to tight the
belt
Picture 6.2.6

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6.2.1.2.2 The replacement of electric motor
Step 1 : After Screwing the two screws in picture 6.2.77A and then unpluging
the cable from the socket in picture 6.2.7B, you can take the motor out of the
sample loader.
Step 2: When replacing a new electric motor, you can follow the reverse
procedure in picture 6.2.7 to finish the procedure of replacement.
A B
Picture 6.2.7
6.2.1.2.3 Replace the sample loader device
Step 1: Screw two screws in Picture 6.2.8A and one screw in picture 6.2.8B,
then you can take the sample loader device out of the sample loader (Picture
6.2.8C). After finishing this procedure, you can change a new one to replace it
according to the inverse procedure of dismantling.

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A B
Sample loader device

C
Picture 6.2.8
6.2.1.3 The possible problem and solution for Sample loader
6.2.1.3.1 Sample loader cannot initialize, move right and left
Possible Reason:
1) The confliction between incubation and sample adding position for cuvettes
2) Wrong position between sample loader and incubation
3) Damage of M800-05-E00 board
Solution:
1) Examine whether there are cuvettes in incubation and sample adding

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position previously, which can lead to the conflict between these cuvettes.
2) Examine whether the different position of incubator align with the position
of sample adding and cuvette loader slot.
3) Check whether the indicator LED D15, D16 and D17 located on board
M800-05-E00 change, when the optical sensor of cuvette loader, incubator
and sample adding position was warded off or not. If not, it means that
optical sensor had broken, and you should change a new one.( Picture
6.2.9)
A B
Cuvette loader
sensor
Incubator sensor
Adding Sample
area sensor
Picture 6.2.9
4) When the adding sample device located at incubator position, cuvette
loader and adding sample position, you must make sure block plate on the
belt ward off the optical sensor. If not, this can cause wrong message when
initialization, and in this time you must adjust the position of the sensor.
5) When you adjust the position of incubator sensor in sample loader, there
are two things you must notice: 1, cannot interfere the movement of
incubator, 2, the pin come out of the shrapnel from the cuvette loader
position to incubator, however when the cuvette loader device move from
the incubator position to cuvette loader position, It will move on the upper

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floor. (Picture 6.2.10)
A B
Pin
Moving through this slope
Moving position
Picture 6.2.10
6.2.2 Wash loader
6.2.2.1 The procedure of dismantling Wash loader
Please follow these steps below!

(Please keep the analyzer power off during these processes!)
Step 1: Please take the white panel out of the sample loader (as showed in the
red ellipse of picture 6.2.11);

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This one is the

wash loader
B C
Take this panel

out of socket
Picture 6.2.11
Step 2 : Screw these three screws in red ellipse of picture 6.2.12A; Then you
can pull wash loader out of the position block, and two position block will
appear in front of us (showed in picture 6.2.12B).

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A B
This one is the

sample loader
Picture 6.2.12
6.2.2.2 The replacement and debugging of the component in
Sample loader
6.2.2.2.1 The replacement of the belt in wash loader
Step 1: Just loosen a little of four screws showed in Picture 6.2.13A, and then
the pressure of the belt will become weak （Showed in Picture 6.2.13B） and
you can take the belt out of the motor gear( Showed in picture 6.2.13C ).

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A B
Rotate the belt to

take it off the motor
C gear
Picture 6.2.13
Step 2: Screw the two screws in the red ellipse as showed in Picture 6.2.14A,
and then you can take the belt out from wash loader (Picture 6.2.14B).
A B
Picture 6.2.14

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Step 3: When replacing a new belt, you can follow the procedure (from picture
6.2.14 to picture 6.2.13) and finish the procedure of replacement. Moreover,
after finishing the procedure of replacing belt, you may find the belt may loosen
a little than before, and you can push screws (showed in red ellipse in picture
6.2.15A ) up to make the belt tight like the previous situation and then fix
screws. After finish this procedure, the screw in Picture 6.2.15 B can be fixed
too.
A B
Fix these two screws

Pushes up the four after finish procedure
screws to tight the in picture A
belt
Picture 6.2.15
6.2.2.2.2 The replacement of electric motor
Step 1: Firstly, screw (number 1, 2, 3) three screws in picture and then you
can pull the motor out tightly when you screw number 4 screw (picture
6.2.16A), and unplugging the cable from the socket in picture 6B, you can take
the motor out of the sample loader.

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A 1 2 B
3
4
Picture 6.2.16
Step 2: When replacing a new electric motor, you can follow the reverse
procedure in picture 6.2.16 to finish the procedure of replacement.
6.2.2.2.3 Replace the sample loader device
Step 1: Screw one screw in Picture 7A and five screws in picture 7B, then you
can take the sample loader device out of the sample loader (Picture 7C). After
finishing this procedure, you can change a new one to replace it according to
the inverse procedure of dismantling.

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A B
Picture 6.2.17
6.2.2.3 The possible problem and solution for wash loader
6.2.2.3.1 Sample loader cannot initialize, move right and left
Possible Reason:
4) The confliction between incubation and wash station for cuvettes
5) Wrong position between wash station and incubation
6) Damage of M800-05-E00 board
Solution:
7) Examine whether there are cuvettes in incubation and wash station

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position previously, which can lead to the conflict between these cuvettes.
8) Examine whether the different position of incubator align with the position
of wash station.
9) Check whether the indicator LED D13, D14 located on board M800-05-E00
change, when the optical sensor of cuvette loader, incubator and sample
adding position was warded off or not. If not, it means that optical sensor
had broken, and you should change a new one. (showed in 6.2.18)
A B
Incubator position sensor

Wash station position
sensor
Picture 6.2.18
10) When the adding sample device located at incubator position and wash
station position, you must make sure block plate on the belt ward off the
optical sensor. If not, this can cause wrong message when initialization,
and in this time you must adjust the position of the sensor.

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6.3 Manual of Incubator

This part describes incubator component in detail, including the structure of the
whole component, PCBs that control the incubator, replacement of the whole
component and the driving belt, and troubleshooting.
6.3.1 Overview of the Incubator

Four inner hexagon screws fix the incubator on the supporting rods on the
base board.
Wash
loader
Sample Wash
loader loader
Reflex Sample
sensor loader
Cuvette
loader
M600 incubator M800 incubator
Picture 6.3.1
At the backside of the incubator there is the incubator interface board, which
controls the temperature of the incubator, the heating cushion, the motor, the
encoder, the hall sensor, and the temperature sensor. Encoder records
movement positions of the incubator; while the hall sensor can sense the

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magnet field, then output different voltage signal to control the incubator to find
the initial position.
Power supply
Reflex sensor
M600-03-E05-Incubator Interface (Ver:20121106, connecting on a M600)
Picture 6.3.2
For M600, three plug cables connect to the M600-03-E05 PCB, one for the
reflex sensor above the incubator, one for power supply, and the last one for
the M600-03-E00 PCB.
For M800, there are only two plug cables on the M600-03-E05 PCB, one for
power supply, and the other for the M800-05E00 PCB.
Power supply
M600-03-E05-Incubator Interface (Ver:20130504, connecting on a M800)
Picture 6.3.3

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The components of the incubator are show as below.
3 3
2
4
4
7
5
5
Picture 6.3.4
1. Driving motor
2. Belt
3. Gear wheel component
4. Cable protection sleeve

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5. Incubator base board/clot
6. M600-03-E05-Incubator
7. Driving belt fixing plate
Note: There are 10 incubation slots in M600 and 13 incubation clots in M800.
Only number of slots varys, the basic structure and handling procedures are
almost the same for both models.
6.3.2 Inside of the Incubator
Four screws at each corner fix the incubator to its base. Inside the incubator,
there is one cushion for heating purpose.
Bottom View of the Incubator (M600)
Picture 6.3.5

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Inside the Incubator (M600)
Picture 6.3.6
1. Heating cushion
2. Incubator base
3. GND
4. Temperature sensor
5. Incubation slot
Note: There are 10 slots in the M600 incubator and 13 slots in M800‘s, heated
by the resistance cushion.

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6.3.3 Relevant PCB
6.3.3.1 Layout of the M600-03-E00 PCB
Picture 6.3.7
6.3.3.2 Incubator Related Function on the M600-03-E00 PCB
（1）Incubator temperature control and status indication
（2）Incubator movement control and status indication
（3）Incubator cuvette number scanner

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6.3.3.3 Incubator Related Specification on the M600-03-E00
PCB
Type Label Description Remark
D10 Incubator cuvette detection LED and test green

hole
TP10
When there is cuvette under the sensor, the LED
will on, TP10 voltage <300mV; No cuvette under
the sensor, LED off, Tp10 voltage >4.5V
D12 Incubator initial sensor LED and test hole green
TP11 When incubator at initial position, LED on,

Tp11<300mV
When incubator at other positions, LED off,

TP11 >4.5V
LED D9 Program debugging indicator LED, developers blue

test hole use only
D11 Program debugging indicator LED, developers blue

use only
D16 +3.3V power indicator and test hole yellow
TP14
D15 +5V power indicator and test hole yellow
TP13
D13 +24V power indicator and test hole yellow
TP12
Fuse F1 +24V fuse 5A
Upgrade J4 Upgrade interface

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interface
6.3.3.4 Layout of the M800-05-E00-Multiple Module PCB
Picture 6.3.8
6.4.3.5 Incubator Related Function on the M800-05-E00 PCB
（1）Incubator control and status indication
6.4.3.6 Incubator Related Specification on the M800-05-E00
PCB
Type Label Description Remark
LED D12 Indicating light and signal testing hole for the Green
sensor of the incubator.
test hole TP10
When the incubator is at the initial position, the

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LED will be on with TP10 voltage < 300mV.
When the incubator is at other positions, the LED

will be off with TP10 voltage >4.5V.
D6 Program debugging indicator LED, developers Blue

use only
D7 Program debugging indicator LED, developers Blue

use only
D2 +24V power indicator and test hole Yellow
TP4
D3 +5V power indicator and test hole Yellow
TP5
D8 +3.3V power indicator and test hole Yellow
TP6
Fuse F1 +24V fuse 5A
Upgrade J4 Upgrade interface

interface
6.4.3.7 Function Description of the M600-03-E05-Incubator
Interface PCB
The PCB is to control incubator temperature, the heating pieces, the motor, the
encoder, the hall sensor, and the temperature sensor.
The encoder records positions the incubator moves to.
The hall sensor can sense the magnet field, and output different voltage
signals to control the incubator in order to find the initial position.

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Picture 6.3.9
6.3.4 Replacement of the Incubator
(1) For M600, the wash loader, the sample loader, and the reflex sensor for
counting cuvettes are just above the incubator; while for M800, the wash
loader and the sample loader are above the incubator. It is not easy to take
out the incubator directly from the front. Therefore, it is better to remove
the back cover and take the incubator from the backside. Disconnect the
cables connected with the incubator, remove four screws on each corner
of incubator, and then you can take the incubator out from the back.
Disconnect

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M600 Incubator (back view)
Picture 6.3.10
(2) Put a new incubator back. Connect the cables and fasten the
screws.Please make sure the screws are securely fastened so that they
will not block the incubator when it is moving. If the incubator‘s height does
not fit for the washer, please adjust the vertical height of the Washer. For
M600, you have to make sure the cuvette can freely go from the incubator
to the wash station and can also move smoothly between the incubator
and the first sampling position. For M800, apart from the above mentioned,
you also have to make sure the cuvette can be pushed from the cuvette
loader to the incubator without any blockage.
Wash station
Incubator
1st Sampling
position
M600 Incubator Height Adjustment
Picture 6.3.11
(3) Login Maglumi Service software to adjust horizontal positions of each
incubator slot according to Chapter 5.1.
6.3.5 Replacement of Driving Belt

(1) Remove the metal plate fixing the belt to the incubator.

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(2) Remove the gear opposite the motor of the driving belt
Picture 6.3.12
(3) Take out the old one through the motor. Assemble back a new one.
6.3.6 Possible Problems and Solutions
6.3.6.1 Warning："Incubator regulation front" or “Incubator
regulation front”
Explanation:
The message means the incubator has not made the required movements in
the predetermined time. (System cycles)
Possible reason:
Incubator forward/backward movements are too slow
Solution:
(1) Check if there is too much rust on the surface of the guide tracks of
incubator. If so, clean the tracks with alcohol. Lubricate the tracks with

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WD-40 or other lubricants.
(2) Check if the driving belt is loose. If so, adjust it or change a new tight one.
6.3.6.2 Warning：“Incubator front move not possible” or
“Incubator back move not possible”
Possible reason:
(1) Something stop the incubator from moving.
(2) Cable/Wire disconnection.
(3) Motor is broken.
Solution：
(1) Check if the incubator gets stuck by something.If so, remove it.
(2) Check if the cables/wires of the incubator are loosen or disconnected.
Observe the flat cable connecting to M600-03-E00/M800-05-E00. Check
its function and connect it again. Check the cables for the motro and
encoder.
(3) Switch off the analyzer, move the incubator manually to feel if there is too
much frictional resistance during the movement.
(4) Switch on the analyzer, see if the incubator can be moved manually. If so,
change the motor.
(5) If the problem still exists, replace M600-03-E00 PCB/M800-05-E00 or the
whole incubator.

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6.3.6.3 Warning：”Incubator not initialized”
Possible Reason：
(1) Hall sensor deformation
(2) Hall sensor broken
Solution：
(1) Turn off the analyzer. Push the incubator to the initialization positionand
turn on the analyzer. When the magnet gets very closed to the hall sensor,
the indicator LED D12 on the M600-03-E00 will be on.
(2) If D12 is not on, use a piece of magnet and place it very close to the hall
sensor. If D12 is on, please correct the hall sensor deformation manually.
However, if D12 is still off, you may have to replace thePCB
M600-03-E00/M800-05-E00 or the PCB M600-03-E05.
(3) If D12 is always on/off no matter where the incubator is located, the hall
sensor may be broken. Change it.
Picture 6.3.13 Hall sensor

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6.3.6.4 Warning：”Temperature ’Incubator’ is out of range！”
Possible Reasons:
(1) Temperature sensor broken
(2) Heating resistance property changed due to aging
Solution：
(1) Recheck the temperature with a high accurate thermometer
(2) If temperature is within the normal range (36.9 ℃±

are 0.5) but there
still
warning messages in the User Software, please calculate the temperature
and save the parameter in the “Errorcard” of the Service Software after
analyzer has been turned on for more than one hour to stabilize.
You can follow steps marked with numbers in picture below.
Picture 6.3.14
(3) If temperature is out of normal range (36.9 ℃+ 0.5), please
potentiometer W1 on the M600-03-E05 PCB to correct the temperature.

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Adjust one circle counterclockwise to increase 0.1 ℃, clockw ise t
decrease 0.1 ℃.
Picture 6.3.15
(4) If temperature of incubator is far away from the normal range, you have to
check the temperature sensor, or change the M600-03-E05 PCB.
(5) If after changing M600-03-E05 the problem is still there, you may have to
change the incubator.
6.3.7 Maintenance
I. Clean the surface of the incubator once a month or when necessary.
II. Check the horizontal positions of each slots of the incubator every 6
months.

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6.4 Manual of Washer

This document is illustrating how to replace the Washer component, adjust of
wash station, and demonstrate the most potential issues may occur in Washer
unit, related error message and maintenance procedure.
6.4.1 How to disassemble the Washer component
As the picture shown below, the whole Washer component includes: transport
channel; back transport channel; washer lift (Picture 6.4.1).
Washer Component (1) transport channel; (2) back transport channel;
(3) washer lift
Picture 6.4.1
(1) Switch off the instrument and manually take out the wash lift. Please take
care as there are several tubes on the washer lift.

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(2) There are 4 screws on the Washer area shown in the picture below (Picture
6.4.2), one is under the washer lift. As you can directly find there is a black
soft cover in the back used to protect the internal PCB (03-E01) and signal
lines. Release the cable before you take the Washer component out.
Screws used to fix the Washer component
Picture 6.4.2
(3) Take out the Washer component gently.
(4) Install a new Washer component back to right position and fix the screws.
The height of Washer should be matched to surrounding components such
as: incubator, pusher. Otherwise cuvettes may be stuck during the
transport.
6.4.2 How to replace a new transport rack
The white rack (which is also called: transport rack) beside the channel is used
to push the cuvette forward during the test.

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(1) There are 2 gears used to control the transport rack, and these gears are
fixed by relative screws shown below (Picture 6.4.3). Loosen the screws
and gears.
Gears and screws for transport rack
Picture 6.4.3
(2) Take out the transport rack and replace it. Please take care of the sensor.
(For Maglumi 600, before you replace a new rack, please cut off one tooth at
left ende because the rack for Maglumi 600 is one tooth shorter )
(3) Release the sensor, loose the screws fixed to the sensor and move away.
(4) As Picture 6.4.4 illustrates below, take out the transport rack carefully.
Remove the transport rack
Picture 6.4.4

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(5) Insert a new transport rack into right position, move back the sensor and fix
it. As shown in Picture 6.4.5, the end of transport rack should match the
edge of channel. And the sensor seperation blade should be right inside
the U type sensor.
The transport rack, channel and sensor seperation blade.
Picture 6.4.5
6.4.3 How to replace a new Washer transport channel?
(1) Take out the Washer component according to Picture 6.4.6, there are two
screws used to fix the Washer transport channel. Please release these
screws.
Two screws are at the bottom of the Washer component
Picture 6.4.6

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(2) Use a hammer to hit one end of the channel, it could be moved out a little
bit. Then use a screwdriver to release channel which demonstrated in
Picture 6.4.7 below. Please take care of your hands.
Use a screwdriver to lever the channel
Picture 6.4.7
(3) Insert a new transport channel to right position and fix the screws.
6.4.4 How to replace the reed and steel beads?
There are 3 groups of reeds and springs inside the wash transport channel
used to avoid the cuvette segment from moving backward (Pciture 6.4.8).
Single reed. Inside there are one spring and two steel beads
Picture 6.4.8

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(1) Take out the transport channel. There is a hexagon screw used to fix the
reed, loosen it. Beware of the tiny spring and steel beads inside which are
very easy to lose.
The structure of this area is like:
Channel
Big Bead
Small
Reed
(2) Replace the spare part in order and then fix the screws.
6.4.5 How to replace the magnets?
There is a magnetic field by the side of transport channel fixed by two screws
(Picture 6.4.9). Totally there are 9 groups of permanent magnets inside
covered by a metal plate.
Two screws and metal plate with magnet units inside
Picture 6.4.9
(1) Take out the transport channel and loose these two hexagon screws.

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(2) Directly remove the metal cover. The magnets units are fixed on the
surface, you need to replace the whole metal plate.
6.4.6 How to replace the washer lift?
The washer lift is not fixed on the Washer component; you can directly take it
out. Please mind the tubes.
(1) Switch off the instrument and take out the washer lift carefully.
(2) Disconnect the tubes and the relative detector cable.
6.4.7 How to replace the wash needle?
Totally there are 3 pairs of wash needles in one wash lift. Cooperate with the
reed, wash needles can bounce up for 1-2mm after they touch the bottom of
the transport channel.
(1) Turn off the instrument and take out the wash station.
(2) There are 4 hexagon screws on the front of washer lift (Picture 6.4.10),
loose them and remove the front cover of the needle unit.

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Wash needle group and 4 screws
Pciture 6.4.10
(3) Pull out the tubes and remove the prior wash needle.
(4) Insert the new wash needle into right position and make it locked in the
reed.
(5) Install the front cover and fix the screws. Put the wash lift back to right
position.
6.4.8 How to replace the back transport push rod?

The back transport push rod is not fixed on the Washer, you can directly
remove it.
(1) Switch off the instrument and remove the push rod.
(2) The normal position of push rod is illustrated in Picture 6.4.11; the rack
should be 90 degree. Otherwise the motor cannot match the rack.

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The rack should be 90 degree ensures to match with the motor
Picture 6.4.11
6.4.9 How to replace the motor in the Washer area?
Totally there are 3 motors in Washer, one is for transport rack (A, Picture
6.4.13); one is for washer lift (B, Picture 6.4.12), the other one is for trandport
rack. Please take a photo before disassembling/replacing a new motor,
including the cable allocation and motor position.
A
The motors. A: the motor controls the transport rack;
B: the motor controls the washer lift (with a encoder)
Picture 6.4.13
Motor A – controling the movement of the transport rack.

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(1) Take out the Washer and disassemble the transport rack.
(2) There are 2 hexagon screws for this motor (Picture 6.4.14). One is beside
the back transport channel, the other one is under the transport channel.
Release the screws and take out the motor.
Two screws for this motor
Picture 6.4.14
Motor B – controling the movement of the washer lift.
(1) Take out the Washer.
(2) There is a metal plate used to hold this motor and using two screws to fix it
(Picture 6.4.15). Loose the screws and disconnect the cable of encoder
and take out the motor.

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Two screws on the metal place used to fix this motor
Picture 6.5.15
6.4.10 How to replace the location block?
As shown in Picture 6.4.16, there are 5 blocks at the edge of Washer transport
channel used to avoid cuvette from moving backwards.
Top view of the transport channel
Picture 6.4.16
(1) Take out the wash transport channel.
The structure of this area is like:

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Blocker
Spring
Shelf
Cover
The spring is inside the block and the blocker is perched on the shelf.
(2) Loose the screws and take off the cover, shelf and blocker.
(3) Install the spring and block to the right position (Picture 6.4.17).
(4) Install the shelf and cover back and fix the screws.
Use a forceps manually inserts the spring and blocker to right position
Pciture 6.4.17
6.4.11 Adjustment of Washer lift position in service
software
There are 4 washer lift position (1) Initialize; (2) Inject; (3) Suction; (4) Prime,
shown in Picture 6.4.18.

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The Washer Lift interface in service software
Picture 6.4.18
[Notice: the ‘Parameter’ is fixed, do not change it.]
(1) Initialize: This position is the home position of washer lift during the test, not
the initial position after initialization. Push a cuvette into the middle of the
washer. The washer aspirate needles should be 2-3mm higher than the
cuvette.
(2) Inject: This position is the injection position for washer needles. Push a
cuvette into the middle of the washer. The washer aspirate needles shall
match the cuvette level.
(3) Suction: This is the aspiration position; used to remove the liquid in the
cuvette vial. Push a cuvette into the middle of the washer. The washer

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aspirate needle should touch the bottom of the cuvette and the needle reed
should be springback for 1mm.
(4) Prime: This position is the prime position for wash needle. Remove the
cuvette and make the aspirate needle touch the bottom of the transport
channel. The needle reed should be springback about 1mm.
6.4.12 Problem Shooting
6.4.12.1 Washer Loader
Error message: (01040001) Washer loader cannot move forward!
(01040002) Washer loader cannot move back!
(01040003) Washer loader not initialized!
Solution:
(1) Kindly check whether there is any dust blocks the washer loader hole.
(2) Manually rotate to motor to check whether there is any mechanical
resistance.
(3) Check the relative height of incubator and washer.
(4) Check the incubator position in service software.
(5) Make sure the loader rack is properly matched to the motor.
(6) Change a new PCB (01-E00).

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6.4.12.2 Wash transport
Error message: (03010001) Wash transport not initialized!
(03010002, 03010003) Wash transport cannot move!
Solution:
(1) Check the signal line between Washer and big back panel.
(2) Check the performance of the rack sensor. D8 LED on 03-E00 PCB will be
off while the metal plate on the rack blocks the optocoupler.
(3) Adjust the sensor position
6.4.12.3 Wash lift
Error message: (03020001) Wash lift not initialized!
(03020002) Wash lift regulation up!
(03020003) Wash lift regulation down!
(03020004) Wash lift cannot move up!
(03020005) Wash lift cannot move down!
Solution:
(1) Apply some WD40 on wash lift rack, axis and axle bearing.
(2) Make sure the connection of every cable including the encoder and motor
is good.
(3) Check the performance of hall-sensor. Turn off the instrument  Switch on
the main-switch  hold a magnet close to the hall-sensor (Picturte 6.4.19).

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If D9 LED on 03-E00 PCB is on which means the position of hall-sensor is
abnormal, then manually change the position.
If D9 LED is still off, change the 03-E00 or 03-E02 PCB.
If D9 LED always remains on or off, this sensor is broken.
Hall-sensor for washer lift
Picture 6.4.19
(4) If instrument is power on, change the 03-E00 PCB.
6.4.12.4 Back transport
Error message: (03030001) Backtrans not initialized!
(03030002) Backtransport cannot move left!
(03030003) Backtransport cannot move right!
Solution:
(1) Make sure incubator, backtransport channel and pusher are in the same
level.
(2) Adjust the pusher position.
(3) Check the cable between Washer and big back panel.

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(4) Adjust the optocoupler position.
(5) Kindly check whether there is any dust blocking the transport rod hole.
(6) If the transport rod is loose when the instrument is power on, change the
03-E00.
6.4.12.5 Wash needle
Error message:
Washer Injector inject abnormally. Please shut down the analyzer and check
carefully!
Solution:
(1) Check the connection of tubing system.
(2) Check whether there is any air leakage in valves and tubes.
(3) Use an syringe to clean the wash needle internal space.
(4) Check the performance of the wash pump and relative 3–way valve.
6.4.12.6 Wash soak
Error message: Washer peristaltic pump soak abnormally!
Solution：
(1) Use an injector or acupuncture needle to clean the internal space of
aspirate needle.
(2) Check the connection condition of tubing system.

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(3) Change a new group of tube for peristaltic pump.
6.4.12.7 Cuvette stuck in Washer Loader
If cuvette stuck in Washer, or totally cannot move forward, the experiment will
stop. Please carefully observe the washer transport gear when transport rack
moves back.
Case1: Transport gear cannot cross over the location gap on the cuvette
(Picture 6.4.20).
Top view of Case1
Picture 6.4.20
Solution:
Increase the min-steps in service software:
Service software initializewash trans Increase the Mini steps value by
20Save (Picture 6.4.22).

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Adjust the transport rack in service software
Picture 6.5.21
Case2: Transport gear cannot touch the cuvette slot at Y-axis:
Solution:
Move the gear touch the cuvette a little by 0.1mm at Y-axis by adjusting gear
fixing screws (Picture 6.4.22).

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The screws and the transport gear
Picture 6.4.22
Case3: Cuvettes stop in the entrance of the channel.
Solution:
Check the position of entrance sensor (Picture 6.4.23), loose the screws and
make sure the sensor will not block the path.
The right position for entrance sensor
Picture 6.4.23
Case4: Cuvettes stop in the connection area of each component.

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Solution: Check the horizontal level of each component. Adjust by loose the
screws.
6.4.12.8 “Washer peristaltic pump soak abnormally!”
Case1: There is a function to avoid carryover in the Washer area during the
wash. 3 groups of signal lines are connected with the corresponding needles,
each group has two needles connected with aspirate and dispense needles
respectively (Picture 6.4.24).
Once there is any carryover/ droplet, two signal lines will be short circuited and
the instrument will give an error message.
The signal lines use to avoid carryover
Picture 6.4.24
As we can see, there are two types of cables, the white one and the black one.
The white one is use to connect with all the dispense needles.
The black one is use to connect with all the aspirate needles.
Solution: reconnect the signal to right position.
6.4.13 Preventive Maintenance
1. Transport gear for Washer Every 6 – 12 months

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2. Wash needle assembly Every year

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6.5 Mannual of Pusher
This part is to illustrate the replacement of pusher and components of it like
motor, belt and the like. Moreover, constitution of circuit board of the pusher
and troubleshooting are introduced.
6.5.1 The procedure of dismantling pusher

(1) Please take the white flat cable out of the pusher:

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Flat cable
Picture 6.5.1
(2) Loose the three screws in red circle of Picture 6.5.2;
Picture 6.5.2
(4) Finally, three supporting rods will appear in front of us (as showed in
Picture 6.5.3), and we can take the pusher out of the analyzer depicted in
Picture 6.5.3.

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A B
Three screw holes

Pusher
Picture 6.5.3
6.5.2 The replacement of the component in pusher
In this section, as our company has two different model of pusher in sequence-
the old one and the new one, this part will respectively instruct how to replace
the component of the new model of pusher and the old one.
6.5.2.1 The replacement of the belt in pusher
For the new model:
( 1) Take the screw out of the screw holes labeled in Picture 6.5.4 and do as
the sequence of the alphabet (A→B→C). In the end, the old belt can be
replace by a new one.

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A B
C D
Take these screws out
Picture 6.5.4
( 2) The new belt must be first put into spout (as can be seen in Picture 6.5.5
A)
and then put and fix the cuvette loader on it showed in Picture 6.5.5B;
A B
Put the belt into the gear
Fix the cuveete loader

onto belt and tighten the
screws

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Picture 6.5.5
(3) Put the other side of the belt into the box as depicted in picture 6.5.6A.
And fix the four screws which can determine the loose extent of belt into
the screw hole (in Picture 6.5.6B) and put the two screws able to
min-adjust the loose extent of the belt on the other side into the screw
holes (showed in Picture 6.5.6B);
A B
Two screws determine the

position of the belt Two screws min-adjust the
loose extent of the belt
Picture 6.5.6
For the old model:
(1) It is also the same as the new one which use the three screws to fix it on
the holder rods. When putting these three screws away from the holder, the
pusher will appear in front of you (showed in Picture 6.5.7).

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Picture 6.5.7
(2) There is something different between the new one and the old one when
you put the belt away from the pusher. As you can see in Picture 6.5.8, the
screws of the electric motor must be put away (Picture 6.5.8A), and rotate it as
the clockwise of 15o (Picture 6.5.8B), and then the belt can be taken out of the
pusher (Picture 6.5.8C).

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A B
Rotating 15o
Screw
Belt
Picture 6.5.8
(3) When replacing a new belt, you can follow the reverse procedure in (2)
and finish the procedure of replacement. Moreover, after finishing the
procedure of belt procedure, you may find the belt may loosen a little than
before, and the spring cushion can be bended to make the belt tight like
the previous situation (followed the procedure in Picture 6.5.9).
Picture 6.5.9

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6.5.2.2 Adjustment of pusher belts
Check the belt on the cuvette holder. If it is loose, take out the pusher, and
tighten the belt as instructed by the picture below. Please be noted that there
are two different kinds of pushers. For the old design, please refer to Picture A.
For the new design, please refer to Picture B.
1. Take out the sheetmetal.
2. Bend to the proper curve.
3. Install it back.
Picture 6.5.10

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1. There’re 4 cross recessed pan head

screws on the side of the metal holder.
Loosen them
2. Adjust the belt with the 2 hexagon
socket screws to make sure the belt is
neither too tight nor too loose.
3. After adjustment of the belt, fasten the

cross recessed pan head screws again.
Picture 6.5.11
6.5.2.3 Replacement of electric motor
For the new model:
(1) Screw these two screws and rotate 15o counterclockwise, then the
electrical motor can be moved away from the pusher (showed in Picture
6.5.12).

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A B
Two screws
Cutting down the cable Rotating 15o
Pictue 6.5.12
(2) Put the cable connected to electric motor away from the board (showed in
Picture 6.5.13).
A B
Cutting down the cable
Cutting down the cable
Picture 6.5.13
(3) Screw these two screws away from the electric motor, and then the electric
motor will appear in front of you (showed in Picture 6.5.14).

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A B
Screw these two screws
Picture 6.5.14
(4) You can replace a new electric motor and follow the inverse procedure of
dismantling the motor to install a new one.
For the old model:
The procedure of dismantling the old model is the same as the new one, so
you can refer to the part of the electric motor replacement for the new electric
motor.

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6.5.3 How to adjust the position of the components in
pusher
6.5.3.1 The adjustment of height between the pusher and
washer
(1) If you find the cuvettes unable to pass from the washer transport to the
pusher smoothly (showed in picture6.5.15 A), the four screws on the bottom
of the washer can be adjusted to match the height with the pusher
(according to Picture 6.5.15B).
Rotate these four

A B
screws to adjust the
height of the washer
Picture 6.5.15

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6.5.3.2 The adjustment of pusher position.
Picture 6.5.16
Adjustment Steps ：Referring to interface in Picture 6.5.16
Initialization:
(1) Check the fixed parameters. Check the fixed parameters: Regulation 50,
Frequency 1600. Click <Writeparam> button to save the parameters;
(2) Click <Init> button to make sure the washer lift can work normally.
Backtransport:
(1) Put a cuvette in the pusher. Select <Init/Backtransport> to check box in the
“target position” dialogue to move the pusher to the backtransport position;

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(2) Move the stepsize bar in the “Teach pusher” dialogue to choose the value
of the “stepsize”:
(3) Click to adjust the pusher’s Init/Backtransport position. Observe if

the cuvette can get into the bancktransport channel smoothly from the
pusher, and then click <Writeparam> button to save the parameters.
Washer：
(1) Select <Chamber> check box in the “target position” dialogue to move the
pusher to the washer position;
of the “stepsize”;
(3) Click to adjust the pusher’s washer position. Observe if the cuvette
can get into the washer channel smoothly from the pusher, then click
<Writeparam> button to save the parameters.
Chamber:
(1) Select <Chamber> check box in the “target position” dialogue to move the
pusher to the chamber position;
of the “stepsize”;
(3) Click to adjust the pusher’s Chamber position. Observe if the

cuvette can get into the chamber channel smoothly from the pusher, then
click <Writeparam> button to save the parameters.

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6.5.4 Component 02-Incubator and Pusher
6.5.4.1 Layout of the 02-E00 PCB
02-E00 PCB component view
Picture 6.5.17
6.5.4.2 02-E00 PCB Functioning
（1）Temperature ontrol of the incubator and status display
（2）Movement control of the incubator and status display
（3）Movement control of the pusherr and status display

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6.5.4.3 02-E00 PCB electronic component list
Type Marked Explanatory Notes Remarks

Symbol
D6 Incubator sensor LED green
The LED is on when the incubator is located

at the initial position.
D5 Back transport sensor LED green
The LED is off when the back transport is

located at initial position ,blocking the optical
sensor
D2 Heating LED green

LED
The light is on when the incubator is in
heating state .
D10 Program debugging LED，should only be blue

used by program developer
D7 3.3V Power LED yellow
D8 5V Power LED yellow
D9 24V Power LED yellow
F1 24V Power Fuse 5A

Fuse
F2 5V Power Fuse 3A
Interface J20 Interface for upgrade the program
6.5.5 Problem shooting

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6.5.5.1 “Pusher not initialized”
Phenomenon:
The analyzer alarms and stops with a warning message “pusher not
initialized”.
Possible Reason:
(1) Optical sensor broken or wire disconnection
(2) Outside interference
Solution:
(1) Check the output voltage for optical sensor. To measure the voltage of the
solder junction (yellow wire, see below Picture 6.5.18), normally it’s over
4V when optical sensor is warded off by pusher slot in initial position(at the
same time, the indicator LED D5 located at 02-E00 PCB become on),
otherwise it’s less than 0.5V. If the value is out of range, change the
02-E03 interface PCB to fix the problem.

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A B
02-E03 board
02-E00 board
LED D5 on or off
The yellow wire
Picture 6.5.18
(2) Check the motor/encoder wire connection and fix it if loosen. Keep the
cable of
02-E03 interface PCB away from strong power source to avoid
electronic interference.
6.5.5.2 The wrong position of the pusher movement within
specified time
Phenomenon:
The analyzer alarms and stops with a warning message “pusher regulation
back”.
Possible Reason：
(1) Mechanical resistance outside

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(2) Wire/socket disconnection or loosen to encoder
(3) Wrong positioning due to rotating bell aging.
Solution：
(1) Check the main cover, Washer Channel, Chamber Transport if there is
mechanical
(2) Force /resistance against the Pusher. If necessary, adjust the parts
accordingly.
(3) Check the wire/socket connection of encoder and fix it if loosen
(4) Disassemble the pusher and adjust the metal belt tension. Then check the
position in
(5) Service software, following the direction of adjustment.
6.5.6 The maintenance of pusher
Notes: The interval of this maintenance is one year
(1) Clean orbit
(2) Check plastic orientation block
(3) Check calibration (washer, measuring chamber,backward device ).

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6.6 Manual of Chamber

This part illustrates the replacement of chamber, component disassembling,
adjustment and replacement along with trouble shooting and maintenance.
6.6.1 How to disassemble the chamber
（1）Take the front panels and back panel out of the analyzer, which will allow
you the access to the chamber from different angles.
（2）Unplug these three cables (see Picture 6.6.1) at the back of chamber, and
remove the black cover (with a red LED in the front) of chamber. The
chamber lift will be in front of you (see Picture 6.6.2).
Picture 6.6.1

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Picture 6.6.2
（3）Remove tubes connecting to the chamber lift, namely, tubes of Starter 1,
Starter 2, and the waste.
(4) Loosen the cross-head screw on starter injector (see Picture 6.6.2), and
then softly loosen the metal connectors of starter tubes as shown below,
and take connectors away from the tubes.
Picture 6.6.3

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Picture 6.6.4
(5) Pull the waste tube out of metal suction head, and then pull the other end
out. See Picture 6.6.5 for the detail.
Picture 6.6.5
Note: Leave signal cable alone. Do not remove it!
(1) Unscrew three screws at the back of chamber to release the tubes (Picture
6.6.6).

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Picture 6.6.6
Note: When removing these three screws, pay attention to black ‘O’ ring in
each tube, three in total. Keep them carefully!
(2) Remove the PMT away from the measuring chamber.
Please remove the signal cable (a black round cable on the right of PMT)
first! Then follow steps in Picture 6.6.8 below.
Picture 6.6.8

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Note: It is highly suggested that you cover PMT with a black bag in order to
avoid direct light to the PMT.
(3) Unscrew 3 screws shown in Picture 6.6.9 to take the chamber out of the
base.
Picture 6.6.9
The supporting rods of the chamber will appear as in Picture 6.6.10.
Picture 6.6.10
Now you have a list of small parts as in Picture 6.6.11.

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Picture 6.6.11
6.6.2 How to replace the components in chamber
6.6.2.1 How to replace the white starter injector
Connect the starter tubes and the waste tube to starter injector, and fix the
screws. Remember to take ‘O’ rings into account. Refer to 6.7.1 for detail.
Notes: 1. Tubes are supposed to be 1 to 1.5 mm out of the injector. Pay
attention when installing tubes back!
2. Starter connectors are aslant screwed into the injector rather than uprightly.
3. There is one red ‘O’ ring in each hole of the injector. Please pay attention.
6.6.2.2 How to replace the chamber glass
(1) Refer to 6.7.1 for detail. Remove the three parts in Picture 6.6.11 below,
and then take the chamber lift out. Dismantle the chamber window with the
chamber glass (as showed in Picture 6.6.12).

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Picture 6.6.11
Picture 6.6.12

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(2) Remove the screw out of the lift, and the old chamber glass will appear in
front of you (as showed in Picture 6.6.13). A new chamber glass can
replace this old one.
Picture 6.6.13
6.6.2.3 How to replace the chamber transport belt
(1) Refer to the procedure in Part 1. After being taken out of the supporting
rods, the chamber should be placed in the position as shown in Picture
6.7.14, and then you can loosen the four screws fixing the motor and take
out the old belt. Finally, a new belt can replace this old one.
Note: The route of the belt should be in accordance with the position in
Picture 6.6.14.

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Picture 6.6.14
(2) After the installment of belt, the four gears should be adjusted to the
suitable position to ensure cuvettes passing through the channel smoothly.
The position of first three gears No.1, No.2, No.3 should be the same
position (90 degrees) while the last one No.4 is a little larger than 45
degrees (as shown in Picture 6.6.15). If the position of these four gears is
wrong, we should use hexagon screw driver to loosen the screw in the circle
in Picture 6.6.16 and adjust the gear in proper position.
Picture 6.6.15

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Picture 6.6.16
6.6.2.4 How to replace the PMT
Refer to 6.6.1. Put the PMT back to the original position, and then connect all
the cables back to the previous position (Picture 6.6.8). Connect the yellow
ground cable as shown in Picture 6.6.11 below and tighten the screw. Push the
PMT back tightly and firmly into the hole (Picture 6.6.18). Install the back cover
of analyzer back and then tighten all the screws.

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Picture 6.6.17
Picture 6.6.18
6.6.2.5 How to replace a chamber
(1） Take the old chamber out following the instruction in6.6.1.
(2） Fix new chamber onto the base. You can refer to 6.6.1.
(3） Connect the starter tubes and the waste tube to starter injector, and fix
screws and the ‘O’ rings. You can follow 6.6.1 in a reverse way.
(4） Install the PMT back to the chamber. Remember to fasten the GND cable.
You can follow 6.6.1 in a reverse way.

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(5） Connect the signal cable of the PMT, and insert the black chamber cover
back with its blue light cable (Cable 3 in Picture 1) connected back.
Connect the cables shown in （2） in 6.6.1.
(6） Check all the conditions and connections of the whole machine

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6.6.3 How to adjust the position of the components in
the chamber
6.6.3.1 How to adjust the chamber transport
Note: Please make sure the analyzer is switched off and the power supply
cable of PMT, i.e. cable 1 in Picture1, is disconnected before opening the
chamber cover.
Picture 6.6.19
Adjustment Steps ：Referring to interface in Picture 6.6.19
Initialization:
(1）Turn off the analyzer, pull out the connecting cable of PMT, and open the
cover of the chamber.Turn on the analyzer, run the Service software.

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(2）Then press the <Intial> button in the main menu to Initialize the analyzer.
Adjustment of the <Load pos>:
(1）Press the <Chamb trans> button in the main menu to open the “Chamber
Transport” dialog. Changing value of the <Load pos> to adjust the angle of
the transport wheel. Make sure that the transport wheel is 5-10 degrees
clockwise deviation of the 90° position. Save the parameter and go back to
the main menu.
(2） Click the <Pusher> button to open the “pusher” dialog. Move the pusher to
the Init/backtransport position. Then insert a cuvette into the pusher Move
the pusher to Chamber position, observe if the gap of cuvette’s bottom can
clamp the transport wheel smoothly. If not ,read just the value of the <Load
pos>.
Adjustment of the <Adjust pos>:
(1）In the main menu press <Globals> button to open the “Globals” dialog.
Then press the <Meas.cuv> button to open the “Measure cuv” dialog, run
a test to see if the cuvettes can pass through the channel in chamber
soomthly.
(2）Observe process of the “Measure.cuv” carefully in the chamber, check if the
injector of the starter reagent can align with the vial of the cuvette.
(3）If not, we can adjust the <Adjust pos> in the “Chamber transport” dialog to
make the injector can align with cuvette’s vial. (We can also achieve that
purpose by adjusting the position of the injector manually).

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(4）After finishing all the settings, save the parameters. Put a cuvette in the
pusher, move the pusher to the <chamber> position. Then in the “chamber
transport” dialog, input 14 in the <Move count>, and press the <Move>
button to check if the cuvettte can move smoothly.
6.6.3.2 How to adjust the chamber lift
Note: Please make sure the analyzer is switched off and the power supply
cable of PMT, i.e. cable 1 in Picture1, is disconnected before opening the
chamber cover.
Picture 6.6.20
Adjustment Steps ：Referring to the interface in Picture 6.6.20

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Initialization:
(1） Check the fixed parameters） Drop speed 3500; Regulation 1, Frequency
1450, Click <Writeparam> button to save the parameters.
(2） Click <Init> button to make sure the chamber lift can work normally.
Notes: Save setting of parameters first!
Move to Init position:
(1） Select <Init> check box in the “Target position” dialog, Move the stepsize
bar in the “Teach chamber lift” dialogue to choose the value of the
“stepsize”.
(2） Click to adjust the Init position of the chamber lift, make sure
that the shutters of chamber entrance and exit are open. Observe if the
cuvette can get into the chamber from the pusher smoothly, then Click
<Writeparam> button to save the parameters.
Notes: The Init position is not the position where the lift stops after finishing
Initialization. It is a bit lower than it.
Move to transport position:
(1） Select <Transport> check box in the “Target position” dialog, Move the
stepsize bar in the “Teach chamber lift” dialogue to choose the value of
the “stepsize”.
(2） Click to adjust the transport position of the chamber lift, make
sure that the suction needle is higher than the cuvette by 2 mm, and
then Click <Writeparam> button to save the parameters.
Move to injection position:

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(1） Select <Inject> check box in the “Target position” dialog, Move the
the “stepsize”.
(2） Click to adjust the transport position of the chamber lift, make
sure that the injection needle is just above the cuvette by 1 mm then
Click <Writeparam> button to save the parameters.
Move to prime position:
(1） Select <Prime> check box in the “Target position” dialog, Move the
the “stepsize”.
(2） Click to adjust the transport position of the chamber lift, observe
if the suction needle just touch the chamber’s channel’s bottom and
make the spring flake of the needles bend upright by 1mm.
6.6.3.3 The definition of blue light’s RLU in range.
(1） Initial machine in service software, Press the button of Globals >
Background (as can be in the circle in Picture 6.7.21 ).

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Picture 6.6.21
(2) Choose the Ref.LED on, do the test check Blue LED RLU is around the
175,000 (if not in range, change the D/A Ref Led value by 1 or 2, then check
the Ref.LED on RLU function again) as shown in Picture 6.6.22.

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Picture 6.6.22
6.6.3.4 The calculation of PMT high voltage.
(1) Press the Plateau to open the dialogue below. Change the Max RLU to
300000 and press Draw dialogue, then choose the Reference LED on, and
press start. The Suggest D/A highvolt will give you a value. Test 3-5 times
and write the most frequent value in D/A high voltage. Please refer to
Picture 6.6.23.
Picture 6.6.23
6.6.3.5 The measurement of min. value. Cuv
Main point: The Function of the parameter is to test whether there is a cuvette
in the chamber.

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Note: Before measuring the value of min. value. Cuv, we should make sure
value of the "D/A Ref.led" have been confirmed
Picture 6.6.24
(1） Click <Initialization> in the main menu to initialize the analyzer;
(2） Press <Globals>--<Background>Measure the “Ref.LED.on” value for 5
times and record the average value X1;
(3） Move the pusher to the “back transport position”, and put a cuvette in
pusher. Then move the pusher to the “chamber position”;
(4） Move the Chamber Transport for 8 steps to make the cuvette move just
before the PMT in the chamber;
(5） Press <Globals>--<Background> Measure the “Ref.LED.on” value for 5
times and record the average value X2;

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(6） Min.value.cuv = (X1 + X2) / 2. Then fill it in the " Min.value.cuv " and click
"Writeparam"
6.6.4 04-E00 PCB Chamber
6.6.4.1 Function of the 04-E00 PCB
（1）Control of the chamber transport
（2）Control of the chamber lift

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（3）Control of the starter pumps
（4）Control of chamber waste pump
（5）Control of the PMT’s high voltage

04-E00 PCB electronic component list
ID
Type Explanatory Notes Remarks
number
LED D8 Program debugging LED，should only be blue

used by program developer
D11 Indication light of power supply(voltage : 3.3V) yellow
D12 Indication light of power supply(voltage : 5V) yellow
D13 Indication light of power supply (voltage : 24V) yellow
F1 24V Power Fuse 5A

Fuse
F2 5V Power Fuse 3A
upgrade J20 Interface for upgrade the program
Interface
6.6.5 Trouble shooting
6.6.5.1 “Chamber transport not initialized”
Phenomenon:
The analyzer alarms and stops with a warning message “chamber transport
not initialized”

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Possible reasons：
(1) The impact of the external resistance
(2) Damage of optical sensor for the chamber transport
(3) Damage of 04-E01 interface PCB
(4) Damage of 04-E00 PCB
Solution：
(1) Turn the cross-gear manually to check whether there is some resistance
( maybe crystal or outside force).
(2) Pull out the 04-E00 PCB , turn the cross-gear of the chamber transport
manually and check whether the LED D4 on the 04-E01 interface PCB will be
on and off.
If the LED D4 can be on and off normally, the 04-E00 PCB need to be
changed.
If the LED D4 don’t change between on and off, test the voltage of the M5 test
hole on the 04-E01 PCB, change the interface PCB of the optical sensor when
the voltage is abnormal, while change the 04-E01 PCB when the voltage is
normal.

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D4
M5
Picture 6.7.25: the board of 04-E01
6.6.5.2 “Chamber transport move not possible!”
Phenomenon:
The analyzer alarms and stops with a warning message “chamber transport
move not possible”
Possible reasons：
(1) The cross-gears of the chamber transport are blocked by the external
resistance
(2) The angle of the 4 cross-gears is different from the request.
Solution：
(1) Check the position where the cuvettes are esay to be blocked, for example:
entry and exit of the chamber. Observe whether there is outside force.

Rev 20141103
Maglumi® 600/800
(2) Check the relative position of the four cross-gears, the angle of the
previous three cross-gears are the same, aligned with the chamber
channel. The 4th one is 45° different with the others.
6.6.5.3 “Chamber lift not initialized”
Phenomenon:
The analyzer alarms and stops with a warning message “chamber lift
notinitialize”
Possible reasons：
(1) The optical sensor for the chamber lift is damaged.
(2) The position of the chamber lift is too high before initialization.
(3) The breakdown of the 04-E00 PCB or 04-E01 interface PCB.
Solution：
(1) Move the chamber lift manually to block and unblock the optical sensor (as
showed in Picture 6.7.23), check whether the status of the LED D3 on the
04-E01 interface PCB change. (If the sensor works normally, when being
blocked, the LED is on, and when not being blocked, the LED is off.)
(2) If the status of the D3 doesn’t change, test the voltage of the test hole on
the 04-E01 interface PCB. Changing the interface PCB of the optical
sensor when the voltage is abnormal, while change the 04-E01 PCB when
the voltage is normal.
(3) If the status of the D3 changes normally, the 04-E00 PCB needs to be
changed.
(4) The entry and exit of the chamber should not be higher than the chamber

Rev 20141103
Maglumi® 600/800
channel. Otherwise it couldn’t finish initialization.
The optical sensor of the

chamber lift
Picture 6.6.26: Interface PCB of the optical sensor for chamber lift
The light of D3
Picture 6.6.27: board of 04-E01
6.6.5.4 “Chamber lift move up/down not possible”
Phenomenon:
The analyzer alarms and stops with a warning message “chamber lift move
up/down not possible”

Rev 20141103
Maglumi® 600/800
Possible reasons：
(1) Cable of the encoder was loose.
(2) Moving-resistance of the chamber lift is too large.
(3) The waste needle of the chamber is blocked by cuvette.
Solution：
(1) Check whether the cable of the encoder is loose, or bad contact of the
terminal.
(2) Check whether the chamber lift is rusty, and clean it.
(3) Turn off the analyzer, move the chamber lift manually to check whether
there is great resistance; Observe whether there are crystals besides the
exit and entry of the door; If the chamber lift always alarm at the same
position, check the mechanism resistance.
(4) Pull out the cable of the PMT, open the cover of the chamber, do the
“Measure Cuv” to observe whether each vial of the cuvette is just below
the injector. Or whether the vial will be blocked by the suction needle, if
blocked, adjust the parameter of the chamber transport or position of the
injector.
(5) If the cables and resistance are all normal, change the 04-E00 PCB.
6.6.5.5 Problem-detecting of the Pumps concerning the
chamber
A. Start_1/2 not injecting normally.

Rev 20141103
Maglumi® 600/800
Possible reasons：
(1) Cables connected the optical sensor or motor of the starter pump are loose,
or bad contact of the terminal.
(2) Damage of the starter pumps’ optical sensor.
(3) Damage of the 04-E00 PCB.
Solution：
(1) Check cables connected the optical sensor or motor of the starter pump,
and whether the contact of the terminal is OK.
(2) On the back panel, use a multimeter to test the voltage of the white cable
connector for the pump’s optical sensor. When the pump’s sensor is
blocked and unblocked, the difference of the voltage should be more than
4V; if the difference is less than 4V, the pump may be damaged and
should be changed.
The detection of white cable
Picture 6.6.28: white cable connector for the pump’s optical sensor on the
back panel
(3) If there is only problem for one pump, change the two pumps’ cables
connected the optical sensor and motor with each other, and run the start

Rev 20141103
Maglumi® 600/800
pumps again. After changing, if the pump which have problem can work
normally, the 04-E00 PCB need to be changed; If still have problem, the
pump need to be changed.
B. Soak chamber not soak normally
Possible reasons：
(1) Soak pump doesn’t work.
(2) The suction needle or the soak pump is blocked.
(3) The wire between the suction needle and detection needle is loose or be a
short circuit.
(4) Water drop between the suction needle and detection needle.
(5) There is no height difference between the suction needle and detection
needle.
(6) Damage of 04-E01 or 04-E00 PCB
Solution：
(1) Check whether the two power cables of the soak pump are loose.
(2) Observe whether the pump works when the analyzer does the soak
process. If the pump doesn’t work, test the voltage of the red power cable
(as showed in Picture 6.6.29) with the multimeter. The normal range is
around AC 23V(22V/24V), if the voltage out of range, change the 04-E00
PCB. If the pump works, but the waste cannot be taken out, check whether
the suction needle or the soak pump is blocked by some solid particles.

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Maglumi® 600/800
Red power cable
Picture 6.6.29: soak pump of chamber
(3) If the waste can be taken out by the soak pump, but the software still alarm,
please check the height difference between the suction needle and
detection needle. Actually, the waste needle should be longer than the
detection needle (as showed in Picture 6.6.30). If it is not like this, adjust
the height of the needle with the tool.
Suction needle should be lower

than detection needle
Picture 6.6.30: The suction needle and detection needle of the

chamber
(4) Check the wire. The signal wire connects to the detection needle, and the
grounded needle connects to the suction needle.

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Maglumi® 600/800
The signal wire
The grounded needle
Picture 6.6.31: Wire for liquid level detection
(5) Check whether the LED D2 on the 04-E01 interface PCB at the back of the
chamber will be off when the suction needle and detection needle are in
the liquid. If the D2 will not be off, change the 04-E01 PCB.
(6) If there are water drops between the suction needle and detection needle,
clean them with the cloth.

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Maglumi® 600/800
6.6.6 The maintenance of chamber

Notes: The interval of these maintenance is one year
Check whether sensors of cuvettes are normal.
 Check the connecting device of pipeline of input and output of starter
reagents pump.
 Check the whether the pass in and out of starter reagents pump leaks.
 Clean the motor bearing of starter reagents, lubricate.
 Check the length of the pipeline on the top of injection.
 Check the length of the pipeline of the head of injection and if it is fixed
well (1mm).
 Clean orbit, rising device, motor bearing.
 Clean waste liquid station.
 Clean the head of injection.
 Clean numerical reading window.
 Clean the pipeline of the washer station and measuring chamber.
 Calibrate conveyer (check whether the cuvettes put in the finger).
 Calibrate rising device calibrate the head of injection just up on cuvettes.
 Check PLATEAU (reader stability about four hours). Check background

(<30).
 Check the minimum of cuvette induction.
 Rinse + measure cuvettes 30 times, CV < 5%.

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Maglumi® 600/800
6.7. Sample&Reagent Area of M600 &

Reagent Area of M800
Overview for M600 Sample&Reagent Area(Left)

and M800 Sample Area/Reagent Area(Right)
Picture 6.7.1
6.7.1 M600 Reagent&Sample Area maintenance
6.7.1.1 The procedure of dismantling M600 Reagent&Sample
Area
(1) Disconnect totally 3 cables on the front cover (Picture 6.7.2).

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Maglumi® 600/800
Three cables required to remove

Picture 6.7.2
(2) Please remove the front cover of analyzer (Picture 6.7.3), there are three
of them.
Three screws required to loose

Picture 6.7.3
(3) Loose four screws use to fix the Sample&ReagentArea component
(Picture 6.7.4).

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Maglumi® 600/800
Four screws use to fix the Sample&Reagent Area

Picture 6.7.4
(4) Disconnect all the cables, including: ground, power supply, signal, flat wire
(Picture 6.7.5). Actually, no need to disconnect cable #2 and #3 when take out
this component.
Cables connection in the back of Sample&Reagent Area
Picture 6.7.5

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Maglumi® 600/800
(5) Lift up the M600 Sample&Reagent Are.a gently and manuallyremove the
tube for condensate water in the bottom right corner (Picture 6.7.6).
Condensate water tube for Sample&Reagent Area

Picture 6.7.6
(6) Take out the Sample&Reagent Area and put it on a clean table (Picture
6.6.7)
Sample&Reagent Area separated component
Picture 6.7.7

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Maglumi® 600/800
6.7.1.2 M600-02-E00-Sample&Reagent PCB
M600-02-E00 is use to control Reagent&Sample Area component, you can
find this PCB in the back of the instrument (Picture 6.7.8)
M600-02-E00-Sample&Reagent PCB
Picture 6.7.8
(1) Control of the vertical, inner and outer motor drivers
(2) Control of the bypass pump
(3) Control of the dilutor
(4) Control of the waste pump of pipettor
(5) Control of the receiving process of liquid level detection signal
(6) Sample and reagent track detection
(7) Scan the sample track barcode
(8) Read reagent information from RFID

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Maglumi® 600/800
Type ID Explanation Remarks

D12 Indicating light and signal testing hole for the green
TP12 sensor of the plunger pump (dilutor) .
After initialize the plunger pump, the plunger
reaches the top, and the sensor is not blocked,
at this time the D12 LED is on, and the test
voltage at TP12 is below 300mV;
The sensor is blocked when the plunger is at
other positions, the D12 LED is off, and the test
voltage is above 4.5V
TP13 sensor of shake speed monitoring.
When the sensor of shaker motor is not blocker,
the D13 LED is on, the voltage at TP13 is below
300mV;
When the sensor is blocked by sheet metal, the
D13 is off, and the voltage at TP13 is above
4.5V;
LED D14 Sensor LED for inner pipetting arm initialization green
TP9 and hole for signal test.
When the sensor is not blocked by the sheet
metal on the inner arm, the D14 LED is on, and
the voltage at TP9 is below 300mV;
When the sensor is blocked, the D14 LED is off,
and the voltage at TP9 is above 4.5V
D15 Sensor LED for pipettor’s vertical initialization green

When the sensor is not blocked by the vertical
sheet metal, the D15 LED is on, and the voltage
at TP11 is below 300mV;
D16 Sensor LED for outer pipetting arm initialization green

metal on the outer arm, the D16 LED is on, and

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Maglumi® 600/800
D1 LED for +24V power supply yellow
D4 LED for +3.3V power supply yellow
D5 Program debugging LED, should only be used yellow
by program developer
Fuse F1 +24Vpower fuse 5A
Upgrade J4 Connector for upgrade the left arm
connect
or

Rev 20141103
Maglumi® 600/800
6.7.2 The procedure of dismantling M800 Sample Area
(1) Please loosen 5 screws to remove the front cover of analyzer. Make sure
you have enough space to take the Sample Area out (Picture 6.7.9).
5 screws for M800 front cover

Picture 6.7.9
(2) Loose two screws use to fix the Sample area component (Picture 6.7.10).
Two screws use to fix the Sample Area
Picture 6.7.10
(3) Disconnect two cables, including the signal cable and power supply
(Picture 6.7.11).

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Maglumi® 600/800
Picture 11 The flat cable is signal cable; the green connector is power
supply
Picture 6.7.11
(4) Remove the tube for condensate water in the bottom right corner and
disconnect the door sensor connector (Picture 6.7.12).
Condensate water tube and door sensor connector

Picture 6.7.12
(5) Gently push the Sample Area to the right side, cross the supporting bar,
and lift up whole component (Picture 6.7.13).

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Maglumi® 600/800
Sample Area separated component

Picture 6.7.13
6.7.2.1 The procedure of dismantling M800 Reagent Area
(1) Remove the upper cover of anlyzer, there are 4 screws use to fix this
Reagent
Area component (Picture 6.7.14).
Four screws use to fix the Reagent Area

Picture 6.7.14

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Maglumi® 600/800
(2) In the back of Reagent Area component, you will find several cables;
disconnect left two of them, the flap cable (signal) and two power supplies
(Picture 6.7.15).
Three cables required to disconnect
Picture 6.7.15
(3) Lift up the Reagent Area component, you will find a condensate water tube
in the right lower corner, disconnect it. Also, in the left side, there is a door
sensor cable,disconnect it.Left one is the door sensor, right one is the
condensate water tube
Picture 6.7.16
(4) Take out the Reagent Area and put it on a clean table (Picture 6.7.17).

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Maglumi® 600/800
Picture 6.7.17
6.7.2.3 M800-05-E00-Multiple Modules (Sample&Reagent Area
part)
(1) Sample detection function. LED indicator controlling.
(2) Reagent kit detection. Control the shaker motor.
(3) Barcode reader function. RFID function.
(4) Control separate PCB: M800-05-E01 and M800-05-E02.

D9 Shaker controller indicator and signal test hole. green
TP7 When shaker back to initialize position, or nothing
LED block the optocoupler, LED on, TP7 <200mV;
When optocoupler has been block, LED off,
TP7>4.5V.

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Maglumi® 600/800
D6 Program debugging LED, should only be used blue

D7 Program debugging LED, should only be used blue
TP4
TP5
TP6
Upgrade J4 Connector for upgrade the firmware
connect
or
M800-05-E01-Sample Area Interface
Picture 6.7.18

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Maglumi® 600/800
M800-05-E02-Reagent Area Interface
Picture 6.7.19
6.7.3 Troubleshooting
(2) Unable to read the sample barcode information which is closest or

farthest to the barcode reader
Possible reasons：
1) Barcode reader installation problem
Solutions：
Check whether the barcode reader is installed properly.Barcode
reader should be perpendicular to the instrument bottom. The distance
between laser release point and sample area should be more than 60mm.The
Laser bean should not be blocked by anything.
(3) Unable to recognize sample barcode information

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Maglumi® 600/800
Possible reasons:
1) The barcode lazser size is too narrow
2) The barcode type is not supported by the barcode reader
Solutions:
Read the user manual and check the barcode type and printing width
whether they can be matched by the barcode reader.
(3) Door switch doesn`t work

Possible reasons：
1) Connection cable inappropriate connected
2) Magnetic switch malfunction
3) The magnet inside the Sample/reagent area door unable to be close to
the magnetic switch
4) M600-02-E04 PCB malfunction
Solutions：
1) Check the cable connection between M600-02-E04(or M800-05-E01)
and magnetic switch. Check the flat cable connection to the main PCB board
of the Maglumi analyzer.
2) Hold a magnet material close to the magnetic switch, and the beeper
on M600-02-E00(or M800-05-E01) PCB should have a “beep” sound. At the
same time, it should be short circuit between both cables of the magnetic
switch. If not, the magnetic switch should be replaced.

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Maglumi® 600/800
Magnetic switch for Sample/reagent area

Picture 6.7.20
(4) Sample area cannot detect inserted sample rack
Possible reasons：
1) The head part of the sample rack is too short to be detected by the
sensor
2) M600-02-E04 interface PCB malfunction
3) M800-05-E01 interface PCBmalfunction
4) Sample rack detecting sensor malfunction
Solutions：
Use another sample rack or tool to block the sensor on
M600-02-E04(or M800-05-E01) and check whether it can be detected.
If not, so the sensor on M600-02-E04(or M800-05-E01) is broken and
need to be replaced.

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Maglumi® 600/800
M600-02-E04 PCB board

Picture 6.7.21
M800-05-E01 PCB board

Picture 6.7.22
(5) Abnormal speed of the reagent shaker

Possible reasons：
1) Affected by strong mechanical resistance

Rev 20141103
Maglumi® 600/800
Solutions：
1) Check the cable connection of shaker motor and 06-E02(or 05-E02)
bonding wire
2) If you can move the shaker freely and feel no resistance, this indicates
M600-02-E00(or M800-05-E00) malfunction and need to be replaced
3) If the shaker blocked when move to a certain position, this indicates this
position exist some mechanical resistance.
4) If there is a warning even the shaker moving normal, use the
multi-meter to measure the voltage at TP13 testing hole in M600-02-E00 (or
M800-05-E00).
06-E02 shaker speed detecting PCB board

Picture 6.7.23
(6) Cooling function in reagent sample/reagent area not work

properly
Possible reasons：
1) Temperature sensor malfunction
2) Fan malfunction
4) M800-05-E02PCB malfunction
5) Peltier malfunction
Solutions：

Rev 20141103
Maglumi® 600/800
1) Use multi-meter to measure the yellow signal wire at J4,to check the
output signal of the temperature sensor.
2) Check whether the fan can work normally. If it doesn`t move, then
check the voltage of J8 socket. If the voltage is +24 Volts, then you need to
replace the fan.
3) Check the voltage between red and black wire of J3 socket. If the
voltage is +24V, it indicates that the peltier is failure and need to be replaced. If
there is no voltage or the voltage is very low, then replace M600-02-E04 (or
M800-05-E02) interface board.
(7) Cannot detect inserted Reagent

Possible reasons：
1) Head part of reagent kit (which is used for presse the detector switch)
is too short.
2) M600-02-E04 interface PCB malfunction
3) M800-05-E02interface PCB malfunction
Solutions：
Use another reagent box or tool to press the switch on M600-02-E04 (or
M800-05-E02) to check whether it can be detected. If not, then this indicates
that the switch malfunction and need to be replaced.

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Maglumi® 600/800
6.8 Manual of Pipettor module

6.8.1 Overview
This part is to illustrate the replacement of Maglumi 600/800 pipettor and
components of it, such as motor, arms, belt and so on. Moreover, constitution
of circuit board of the pipettor and trouble shooting are introduced.
Pipettor of Maglumi600
Picture 6.8.1

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Maglumi® 600/800
Pipettor of Maglumi800
Picture 6.8.2
6.8.2.How to disassemble the pipettor
6.8.2.1 How to disassemble the pipettor(M600)
1), Loosen the screw of horizontal panel, vertical panel, left panel, front cover
and back panel.

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Maglumi® 600/800
(External Panels of Maglumi600)

Picture 6.8.3
2), For the front panel, there are four screws in total. Please loosen them,
disconnect the cable and take the cover away.
(Procudure of removing external panels)
Picture 6.8.3

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Maglumi® 600/800
3), Loosen the two screws, and take the holder out.
(Side panel holder)

Picture 6.8.4
4), Loosen the two screws at the bottom of the pipettor, dismantle the two
cable and the tube joint, and then take the whole pipettor out.

Rev 20141103
Maglumi® 600/800
(Removing procedures of M600 pipetting arm)
Picture 6.8.5
6.8.2.2 How to disassemble the pipettor(M800)
1),Romvoe horizontal panel and front panel as shown below;

Rev 20141103
Maglumi® 600/800
(External panels of Maglumi800)

Picture 6.8.6
2), Unplug two flat cables below and disconnect connector for hard pipetting
tube;
(Removing procedures of M800 pipetting arm)

Picture 6.8.7

Rev 20141103
Maglumi® 600/800
then unscrew four cross screws at the base of pipetting arm;
(M800 popetting arm base fixing screws)

Picture 6.8.8
Finally you will see as below:
(Top View after removement of pipetting arm)

Picture 6.8.9

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Maglumi® 600/800
6.8.3 How to replace and adjust the pipettor
6.8.3.1 How to replace the pipettor of M600/M800
1)Remove panels and get access to pipetting arm;
2)For the front panel, there are four screws in total. Loosen them, disconnect
the cable and take the cover away.
(External panels removement procedures)
Picture 6.8.10
3)Loosen the two screws, and take the holder out.

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Maglumi® 600/800
(M600 side panel holder)
Picture 6.8.11
4)Loosen the two screws at the bottom of the pipettor, dismantle the two cable
and the tube joint, and then take the whole pipettor out. Finally install the new
pipettor accordingly.
For replacement of Maglumi 800 pipettor replacement, please refer to

6.8.2;

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Maglumi® 600/800
6.8.3.2 How to replace the pipettor needle（M600/800）
1), Move the outer arm cover away.
(Removement of outer pipetting arm)

Picture 6.8.12
2), Loosen the screws and the black screw which connects the tube to the
pipettor needle and move it away; loosen the hexagon screw and remove the
black cable; disconnect the blue line by pulling up from the small board.
(Joints inside outer arm )

Picture 6.8.13

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Maglumi® 600/800
3), Loosen the screws marked 1 and 2, and take the pin marked 3 out. Then
take the whole pipettor needle out of the outer arm, and take the previous pin
out.
(Fixing of needle)
Picture 6.8.14
4), Dismantle the old pipettor needle. It can be dismantled into one old needle
and five accessories.
(Details of needle)
Picture 6.8.15

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Maglumi® 600/800
5)Take the needle holder into the outer arm, make sure that the plane of the
holder should be on the right side.
(Needle holder)
Picture 6.8.16
6)Insert the new needle into the needle holder, install the spring and pin, and
fix the needle with the two screws.
(Installation of pipetting needle)

Picture 6.8.17

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Maglumi® 600/800
7)Fix the trigger A and the black line. Make sure that the trigger (A) should be
right above the other trigger (B).
(LLD system )
Picture 6.8.18
8) Connect the blue line to the small board, and rotate the black screw (on the
tube) to the pipettor needle.
(LLD system)
Picture 6.8.19

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Maglumi® 600/800
9) Install the outer arm cover.
(Final view after replacement)

Picture 6.8.20
10) Install the front cover and all the panels according to the above steps.
6.8.3.3 Disassemble and assemble of the outer belt
1) The out-arm belt of M600 is as below.
(View of inside arm)

Picture 6.8.21
2) Remove the front side panel as well as the RFID reader cable

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Maglumi® 600/800
Picture 6.8.22
(Front panel of M600, not for M800)

Picture 6.8.23
3) Then, unscrew the two screws to remove the motor. If the belt is not very
loose and needs some adjustment, it can be adjusted by adjusting the position
of the motor.

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Maglumi® 600/800
Picture 6.8.24
Picture 6.8.25
4) Unscrew the two screws as shown below.
Picture 6.8.26

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Maglumi® 600/800
Now the belt can smoothly go through the motor and cables.
Picture 6.8.27
5) Unscrew the two screws, disconnect the 3 white cables,
Picture 6.8.28
6) Disconnect the black tube connector.

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Maglumi® 600/800
Picture 6.8.29
7) When you finish the above steps, the belt can be smoothly taken off from
the analyzer.
Picture 6.8.30
8) Replace a new belt and assemble it according to the steps above.
Note: As for Maglumi 800, please just do as below:

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Maglumi® 600/800
Picture 6.8.31
Remove the belt and take the belt out, replace it with a new one.
6.8.3.4 How to disassemble and assemble the inner arm belt
1),Take out the pipettor, unscrew four screws of the motor on the back of the
pipettor and adjust the belt by pulling out or pushing in the motor until the belt
is tight enough. Screw them up after adjustment. If the belt is too loose and
needs to be replaced, please take out the motor and follow the following steps.
2),Unscrew two screws of the inner arm sensor along with the cable connector.

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Maglumi® 600/800
Picture 6.8.32
3)Unscrew six screws on the pipettor rack (with four on both of the lateral sides
and two on the back).
Picture 6.8.33

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Maglumi® 600/800
4) Unscrew four black screws connected to the guide rail and the other one on
the catch of the belt.
Picture 6.8.34
5) After the steps above, the pipettor could be dismantled from the pipettor
holder and the belt of inner arm could be taken out and replaced.
Picture 6.8.35
6)Assemble the pipettor according to the step above.
7)As for Maglumi800, we just suggest you to adjust the tension of the inner
arm belt and replace the inner arm motor..
Firstly please release the motor, and then remove the gear wheel out as
shown below:

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Maglumi® 600/800
Picture 6.8.36
Then replace the the moter with a new one, and adjust the position of gear
wheel according to th hight of belt, make sure that the gear fits the belt.
You could adjust the tension of the belt by moving the motor as shown above.
6.8.3.5 How to replace the vertical rod belt of M600.
1), Disassemble the pipettor according to 4.1 to 4.5 (the inner arm belt does
not necessarily need to be taken out).
Picture 6.8.37
2)Unscrew screws and remove the vertical motor on the bottom of the pipettor.

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Maglumi® 600/800
Picture 6.8.38
3), The belt could be easily taken out and replaced.
4), As for Maglumi800, we adopt screw gear rod instead of vertical belt:
Picture 6.8.39

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Maglumi® 600/800
Normally this part is very stable, we just need to do some maintainance job. If
the rod is defective, we just suggest you to replace the whole pipetting
component.
6.8.4.M600-02-E00-Sample&Reagent
TP1 sensor of the plunger pump (dilutor).
2 After initialize the plunger pump, the plunger
reaches the top, and the sensor is not blocked, at
this time the D12 LED is on, and the test voltage
The sensor is blocked when the plunger is at
other positions, the D12 LED is off, and the test
voltage is above 4.5V
TP1 sensor of shake speed monitoring.
3 When the sensor of shaker motor is not blocker,
the D13 LED is on, the voltage at TP13 is below
300mV;
When the sensor is blocked by sheet metal, the
LED D13 is off, and the voltage at TP13 is above
4.5V;
D14 Sensor LED for inner pipetting arm initialization green

metal on the inner arm, the D14 LED is on, and
D15 Sensor LED for pipettor’s vertical initialization green

1 When the sensor is not blocked by the vertical
sheet metal, the D15 LED is on, and the voltage

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D16 Sensor LED for outer pipetting arm initialization green

0 When the sensor is not blocked by the sheet
metal on the outer arm, the D16 LED is on, and
D5 Program debugging LED, should only be used by yellow
program developer
Upgrade J4 Connector for upgrade the left arm
connect
or
6.8.4.1 Functions description of pipettor’s interface PCBs
<1> M600-02-E01 PCB function description
（1）outer and inner pipetting arm, vertical motor and encoder transfer
（2）liquid level detection signal transfer
（1）LLD signal generation

（2）Detection of the outer pipetting arm initialization position by the sensor
（1）Pipetting arm plunger pump motor and sensor signal transfer

（2）Pipetting arm wash pump transfer
（3）Pipetting arm waste pump transfer
（4）Pipetting arm washing control valve transfer

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Maglumi® 600/800
<4>M600-02-E04 PCB function description
（1）Detection of the sample and reagent rack insertion

（2）Detection of the sample/reagent area door switch
（3）Shaker motor and speed monitoring transfer
（4）Control the temperature of sample/reagent area
6.8.4.2. Functions of cable connectors on M600-02-E01
Picture 6.8.40
Connector function Influences or alarms if poorly connected or

damaged
1.M6C13-02-E00 Cable connector It cannot move and alarms ”SampleArm can
J5-2 connected to not be moved down”.
02-E00
2.M6C123-02-E00 Cable connector It cannot move and alarms “SampleArm can
J5-1 connected to not be initialized on vertical”
02-E00
3.Ecncoder arm Cable connector It can moves upwards and downwards a little
out connected to outer bit but soon alarms “The outer track of
arm encoder SampleArm can not be initialized” or “The

Rev 20141103
Maglumi® 600/800
outer track of SampleArm can not move
clockwise”
4. Encoder arm in Cable connector It can moves upwards and downwards a little
connected to inner bit but soon alarms “The inner track of
arm encoder SampleArm can not be initialized”
5. M6c18 02-E02 Cable connector It cannot move and alarms “SampleArm can
J1 connected to not be initialized on vertical”
02-E02
6.M6C26 02-E01 Cable connector The inner arm keeps moving counterclockwise
J5 connected to Inner and alarms “The inner track of SampleArm can
arm sensor not be initialized”.
7.Init Arm V Cable connector It moves downwards a bit and then alarms
connected to “SampleArm can not be initialized on vertical”.
vertical sensor
8.Arm V Motor Cable connector It cannot move and alarms “SampleArm can
connected to not be initialized on vertical”
vertical motor
9.ArmHOut Motor Cable connector It can moves upwards and downwards a little
connected to outer bit but soon alarms “The outer track of
arm motor SampleArm can not be initialized”
10. Arm HIn- Cable connector It can moves upwards and downwards a little
Motor connected to inner bit but soon alarms “The inner track of
arm motor SampleArm can not be initialized”
6.8.5. Problem shooting
6.8.5.1. Pipettor cannot be coordinated(Mainly for M600 , M800
is similar)
Normally, when the pipettor arm is initialized, it will direct to the top-right corner
of the sample & reagent area, and the outer arm and inner arm are in the same
line.

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Maglumi® 600/800
Picture 6.8.41
However, sometimes the pipettor arm's position maybe changed during the
transport, which makes the pipettor arm cannot get to the right position during
initializing. Under this circumstance, please check the pipettor arm carefully as
the following steps.
1), Move the panel away and the pipettor arm cover. There are two screws
under the inner arm to fix the inner arm cover; you need to loosen the two
screws before moving out the inner arm cover.
Picture 6.8.42

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Maglumi® 600/800
2),After moving out the arm cover and the panel, then check the arm belt, both
the inner arm belt and outer arm belt need to be checked. If the belt is
loosened, please tighten it as following steps:
3),For the outer arm belt, please firstly loose the two screws as marked in the
following picture, then move the motor to the left side to make the belt tighten.
At last fix the two screws.
Picture 6.8.43
4), For the inner belt, it is same as the outer arm belt. If it gets loosen, please
firstly loose the four screws, and then move the motor to tighten the belt and fix
the screws.

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Maglumi® 600/800
Picture 6.8.45
5), making sure the two belts are tighten, please log in the service software,
click [Coordinate], the pipettor will initialize. After it finishes initializing, please
check whether the outer arm and inner arm in same line, and the two arm
should direct to the top-right corner of the reagent area. You can refer to the
following picture.

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Maglumi® 600/800
Picture 6.8.46
6), the inner arm is not directing to the top-right corner of the reagent area
when you initialize the pipettor,
Picture 6.8.47
Firstly initialize the pipettor (click [Coordinate]). After initialization, please do
not turn off the analyzer and do not quit the <coordinate>. Just loose the two
screws marked A and B in the above picture, and then move the inner arm to

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Maglumi® 600/800
make it direct to the top-right corner of the reagent area. Then tighten the two
screws.
7), the inner arm is directing to the top-right corner of the reagent area when
you initialize the pipettor, but the outer arm is not in the same line with the
inner arm,
Picture 6.8.48
Firstly, initialize the pipettor and do not turn off the analyzer and also do not
quit the <coordinate>. There are two screws in the wheel gear of the outer arm.
Loose the two screws as the above picture indicates, then move the outer arm
to make it in the same line with the inner arm. At last, tighten the two screws
and initialize the pipettor to check whether the pipettor is good.
8),after step 1.1, step 1.2 and steo 1.3, if there is no problem with initialization,
then you can log in the [coordinate], and adjust the needle position. Before you
adjust the needle position, make sure that the needle sensitivity
(Coordinate>>>Inject) value is between 20~30, if the value is not in the range,
you can fill 25 in the area and then click [writeparam].

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Maglumi® 600/800
Picture 6.8.49
9), After the above steps, if the pipettor cannot detect the liquid level when you
adjust the needle position, or the needle cannot get to the right position after
you finish the coordinate. Please check all cables of the pipettor to confirm
whether the cables were connected well or not. Especially the blue line of the
M600-02-E02 PCB in the inner arm.

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B C
Picture 6.8.50
Check the cable of which connected to 02-E02 PCB (Fig A), 20-E01 PCB (Fig
B) and 02-E00 PCB -Sample& Reagent (Fig C).
10), If the cables are connected well, please check the 02-E02 PCB as the
following instructions:
Use a multi-meter (short circuit function) and place the two probes to both
sides of the capacitor to detect if there is short connection (circuit) for the
capacitor.

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Maglumi® 600/800
Picture 6.8.51
Note: There are all 8 capacitors (C1,C2,C3,C4,C5,C6,C7,C8) on this
PCB(M600-02-E02), which have to be checked overall. If there is any
capacitor is detected to be “short circuit” connection, it means that capacitor is
defective.
Picture 6.8.52
After the above steps, generally, the analyzer will be normal. If the pipettor
cannot work still, please contact us.
After everything is normal, then please adjust the needle in the coordinate. For
the Ref.Pos, please make sure that the angle of inner arm and outer arm are
different in the Motion Trace1 and Motion trace 2. For other positions, please
refer the <User manual>.

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For the [Ref.Pos], you must be careful that the Motion Trace 1 and Motion
Trace 2 are different.
Picture 6.8.53
For motion trace 1, the inner arm and the outer arm angle like Fig 1.
Picture 6.8.54

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Maglumi® 600/800
Picture 6.8.55
For motion trace 2, the inner arm and the outer arm angle like Fig 2.
Picture 6.8.56

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For other positions in <coordinate>, please follow the chapter of needle
coordinate.
6.8.5.2. Inner pipetting arm possible problems
6.8.5.2.1 Inner arm unable to initialize(For M600 only)
Possible reasons：
1) initialization sensor is damaged
2) M600-02-E00 PCB is damaged
Solutions：
1) Block or unblock the inner arm initialization sensor with a piece of
light-proof sheet, then check whether the status of D14 changes. If not,
replace the sensor.
Pciture 6.8.57
2) If the sensor is ok, but the motor unable to move and is not locked when
doing inner arm initialization, it is suggested that the M600-02-E00 PCB be
replaced because of damage.

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6.8.5.2.2 Inner arm unable to move clockwise or anti-clockwise
Possible reasons：
1) The power line does not connect the inner arm motor and encoder well.
2) External resistance.
3) The M600-02-E00 PCB or the motor is damaged.
Solutions：
1) Check the wire which is connected to the motor and encoder.
2) Check external resistance by pushing the inner arm manually
3) If the initialization and wire connection are ok, the external resistance does
not exist and the arm is not locked when power on, it suggests that the
inner arm encoder is damaged and need to change it together with the
motor.
6.8.5.2.3. Outer pipetting arm possible problems(For

M600&M800)
1, Outer arm unable to initialize
Possible reasons：
1) Initialization sensor is damaged
2) Wire connected to the interface PCB and motor is loosen
3) M600-02-E00 PCB is damaged
Solutions：
1) Check the wire connected to the interface PCB, motor and adaptor.
2) Pull the outer arm to block or unblock the sensor. Normally, the state of the
D16 LED will change. Otherwise, the sensor is damaged and you need to
change the M600-02-E00 PCB.

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Maglumi® 600/800
Picture 6.8.58
3) If the outer arm cannot move when doing initialization and cannot be
locked when power on, it suggests the M600-02-E00 PCB is damaged .
2,The outer arm cannot move forward or backward
Possible reasons：
1) The power line does not connect the outer arm motor and encoder well.
2) External resistance.
3) The M600-02-E00 PCB or the motor is damaged.
Solutions：
1) Check the wire connected to the motor and encoder.
2) Check external resistance by push the outer arm manually
3) If the initialization and wire connection is ok, the external resistance does
not exist and the arm is not locked when power on, it suggests the outer
arm encoder is damaged and you need to change it together with the
motor.

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Maglumi® 600/800
6.8.5.2.4 Pipetting arm vertical common problems
1, Unable to initialize the vertical direction, the probe cannot move up

during initialization
Possible reasons：
1) The probe hits something in the vertical direction
2) The sensor of the vertical direction is damaged
3) The M600-02-E00 PCB is damaged
4) The vertical motor is damaged
Solutions：
1) Adjust the probe to the correct position if it had hit something
2) Check whether the D15 LED changes its status when the sensor is
blocked and unblocked. If not, the sensor is damaged and need to be
replaced.
Picture 6.8.59
3) If the vertical arm is not locked when power on and cannot move during
initialization, the M600-02-E00 PCB is damaged.
2,Unable to move upward or downward in vertical direction

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Maglumi® 600/800
Possible reasons：
1) External resistance
2) The motor is damaged
3) Bad wire connection
Solutions：
1) Check the wire of vertical motor.
2) Pull the probe in vertical direction and see if there is stronger resistance at
some position.
3) If initialization is ok, the connection is good and resistance is weak, there is
still error warning, indicating that the motor is damaged.
6.8.5.2.5 Pipetting possible problems and solutions
1 Liquid level detection fail
Possible reasons：
1) The signal wire of LLD is not well connected
2) The ground wire of the sample/reagent area is not well connected
3) The metallic membrane on the reagent kit is not removed
4) The LLD parameter is not adjusted correct
5) M600-02-E00/ M600-02-E02 PCB is damaged
6) The pipetting probe crashed or rust
Solutions：
1) Check the LLD signal wire, and check whether there is liquid or crystal on
the adaptor PCB.

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Maglumi® 600/800
Picture 6.8.60
2) Use multi-meter to check whether the ground wire of sample/reagent area
is connected well to the instrument shell.
3) Use the “Detect” function in service software to check the level detect
value. Normally the difference between maximum and minimum value
should be below 3, if it is more than 3, please check the power supply of
the instrument and the grounding or try to replace M600-02-E02 PCB
board.

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Maglumi® 600/800
“Detect” function in service software

Picture 6.8.61
4) If all above are ok, change the sensitivity parameter of LLD gradually(the
value is 25 normally),the sensor becomes less sensitive as the value
decrease.
5) If the needle is clashed, adjust it back to original position.
6) If the needle is rusty, please check whether there are some rust or crystal
material sticks to the welding point of needle PCB board.

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Maglumi® 600/800
6.9 Covers of M600/M800

6.9.1Cover of M600

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Maglumi® 600/800
As the pictures below shows, Maglumi 600 has as much as 9 plates in all.
1
2
3 5
9
6
8

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Maglumi® 600/800
Picture 6.9.1
Usually, we can remove plate1/2/3/4 to observe how the process goes.
1
2
3
5
Picture 6.9.2
Explanation:
---Unscrew 5 mounting screws to remove plate 1 and plate 2
---Then unscrew 2 mouting screws on both sides of plate 3, then remove it.
---Before removing plate 4, pull the plate 5 out.
--- Unscrew 4 mounting screws to remove plate 4.
As the picture below shows, the whole experiment can be observed clearly
after removing plate1/2/3/4.

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Maglumi® 600/800
Picture 6.9.3
If you need to continue detaching the rest panels, please follow the next
procedure.

Rev 20141103
Maglumi® 600/800
6.9.1.1 Plate to cover the front
Front panel
Picture 6.9.4
Picture 6.9.5
Explanation:
.---Unscrew all mounting screws in the front panel.

Rev 20141103
Maglumi® 600/800
---Pull out the cables connected to the panel.
6.9.1.2 Plate to cover the back
Picture 6.9.6
Explanation
---Unscrew all screws on plate 6 to remove it
The top view of cover

Picture 6.9.7

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Maglumi® 600/800
Explanation
--- Remove 4 screws on the cover remove it.
6.9.1.3 Plate to cover to top
Top view of the plate 7

Picture 6.9.8
Explanation
--- Remove all screws underneath the plate 7 to remove it.

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Maglumi® 600/800
6.9.1.4 Plates to cover left(8)&right(9)
Step1 Step2
Picture 6.9.10
Explanation:
---remove all 5 screws in the plate 8

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Maglumi® 600/800
Step1 Step2 Step3
Steps to remove plate 9

Picture 6.9.11
Explanation:
---pull the waste bag container out first.
---unscrew 4 screws underneath the plate.
---unscrew the last 2 screws to remove the plate.
6.9.2 Covers of M800
As the pictures below shows, Maglumi 800 has as much as 10 plates in all.

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Maglumi® 600/800
2
3
8
9
Picture 6.9.12
Usually, we can remove plate1/2/3/4 to observe how the process goes.

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Maglumi® 600/800
1
2
Picture 6.9.13
Explanation:
---Unscrew 3 mounting screws to remove plate 1 or plate 2
---Then unscrew 2 mouting screws on both sides of plate 3
---Before removing plate 4, plate 1, plate 2 and plate 3 have to be removed at
first.
As the picture below shows, the whole experiment can be observed clearly
after removing plate1/2/3/4.

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Maglumi® 600/800
Picture 6.9.14
If you need to continue detaching the rest panels, please read the
following procedure.
6.9.2.1 Plate to cover the front
Front panel

Rev 20141103
Maglumi® 600/800
Picture 6.9.15
Picture 6.9.16
Picture 6.9.17
Explanation:
---Pull out the cables connected to the panel first.
---Unscrew the 10 mounting screws in the front panel.
6.9.2.3 Plate to cover the back

Rev 20141103
Maglumi® 600/800
Picture 6.9.18
Explanation
---Unscrew all screws on plate 5 to remove it
The overlook of plate 6

Picture 6.9.19
Explanation
--- Remove 4 screws on the cross board underneath the plate 6 to remove it.

Rev 20141103
Maglumi® 600/800
6.9.2.4 Plate to cover to top
Top view of the plate 8

Picture 6.9.20
Picture 6.9.21
Explanation
--- Remove all screws underneath the plate 7 to remove it.

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Maglumi® 600/800
6.9.2.5 Plates to cover left(8)&right(9)
8 9
Picture 6.9.22

Rev 20141103
Maglumi® 600/800
Explanation:
---Unscrew the 5 mounting screws on the plate right(8) to remove it.
Picture 6.9.23
Explanation:
---remove 6 screws on the plate left (9)

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Maglumi® Series
Pumps and Tubings
Chapter 7
Pumps and Tubings
7.1 PUMPS ................................................................................................................. 2
7.1.1 DILUTER PUMP.................................................................................................. 2
7.1.1.1 Introduce ................................................................................................. 2
7.1.1.2 How to disassemble and replace the pump ............................................. 3
7.1.1.3 Problem Shooting .................................................................................... 4
7.1.1.4 Volume adjustment of diluter pump ......................................................... 5
7.1.1.5 Maintance of diluter pump ...................................................................... 7
7.1.2 STARTER PUMP ................................................................................................ 7
7.1.2.1 Introduce ................................................................................................. 7
7.1.2.2 How to disassemble and replace the pump ............................................10
7.1.2.3 Problem Shooting ...................................................................................10
7.1.2.4 Volume adjustment of starter pump .......................................................12
7.1.3 VACUUM BYPASS PUMP ....................................................................................14
7.1.3.1 Introduce ................................................................................................14
7.1.3.2 How to disassemble and replace the pump ............................................15
7.1.4 CONDENSATE WATER PUMP .............................................................................17
7.1.4.1 Disassembling of the condensate water pump (Peristaltic pump) ..........17
7.1.4.2 Overview of pump for consendate water (Old design- peristaltic pump).19
7.1.4.3 Structure of the peristaltic pump .............................................................20
7.1.4.4 Maintenance to the peristaltic pump .......................................................21
7.2 TUBING SYSTEM ...............................................................................................25

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Pumps and Tubings
7.1 Pumps
7.1.1 Diluter pump
7.1.1.1 Introduce
The diluter pump is responsible for transporting system liquid to wash needle and for
reagent (sample) aspiration and injection. It consists of motor,sensor,piston bar,in-let
pipe and out-let pipe (Picture 7.1.1,2). The photoelectric sensor can positioning the
Piston bar during the movement (Picture 7.1.3).
Picture 7.1.1
Picture 7.1.2

Rev 20141103
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Pumps and Tubings
Picture 7.1.3
7.1.1.2 How to disassemble and replace the pump

Firstly, loosen two screws (red circle, Picture 7.1.4) on the base, and loosen two
plastic srews (red circle, Picture 7.1.4), note do not confuse the position of the two
plastic screws. Then change your new pump.
Picture 7.1.4

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Pumps and Tubings
7.1.1.3 Problem Shooting
Case 1："Right/Left Needle Inject Pump not initialized"
Problem：
⑴ The last movement of diluter pump beyond the limit position.
⑵ The cable of sensor is loosen.
Solution：
⑴ Check the position of piston bar, if it is beyond the limit position,use
a screwdriver adjust the piston bar (red circle,Picture 5) to the middle position.
Picture 7.1.5
⑵ Check whether the cable of sensor is loosen, and plug in the cable.
Case 2：Right/Left Needle Inject Pump do not work.

Problem：
⑴ The cable of diluter pump is loosen.
⑵ The motor is damaged.
Solution：
⑴ Check the cable of the diluter pump,if it is loosen,plug in the cable.
⑵ change the motor.
Case 3: Liquid leakage of diluter pump.
Problem：
⑴ The plastic screws is loosen.
Solution：

Rev 20141103
Maglumi® Series
Pumps and Tubings
⑴ Check the plastic screws,if it is loosen,fasten it.
7.1.1.4 Volume adjustment of diluter pump

(1)Use an electric balance (correctly calibrated) to measure the weight of an empty
cuvette (assume the weight to be W empty ) and then put it into the left pipetting area
(Picture 6).
Picture 7.1.6
(2) In Maglumi Service, after clicking to initialize all the movable parts
except the pipettor, please click ----- ----- to

initialize the incubator loader to push the cuvette at the left pipetting area to the
exact position (Picture 7).

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Pumps and Tubings
Picture 7.1.7
(3) Click to initialize the pipettor and then the button to enter
the Adjust Inject interface. In this interface, at first you need to set the aspirate
position as the position where you have already prepared some water, for
example, select the 1st track and 1st position of sample area after you place one
tube of water there, the inject position as the left pipetting area and the volume as
40μL.
Picture 7.1.8

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Pumps and Tubings
(4)Click several times to prime the tube and needle thoroughly, then
to aspirate 40 μL water, at last click to inject the water into

the cuvette.
(5)Use the same electric balance to measure the weight of the cuvette with 40μL
water (assume the weight to be W water ) , then we can calculate the weight of the
water: W water -W empty . The value should approximate 40mg.
(6)If do not have electric balance, you can also use a pipettor (correctly calibrated) to
check the volume. Set the volume of the pipettor to be 40μL, and then absorb the
water from the cuvette. Normally, all the water in the cuvette will be absorbed into
the pipette tip without bubble.
7.1.1.5 Maintance of diluter pump

There will be some crystal in the pump after long time of work. We suggest you to
Take off the cap of the pump, and soak it with warm water to remove crystal inside.
7.1.2 Starter pump

7.1.2.1 Introduce
The starter pump is responsible for injecting starters to starter needle. It consists
of motor,interlocks device,sensor,piston bar,in-let pipe and out-let pipe (Picture
7.1.8,9). When the motor moves, it will drive the piston bar in and out under the
action of interlocks device, thus finish aspiration and injection of starters (Picture
7.1.10).The photoelectric sensor can positioning the piston bar during the movement
(Picture 7.1.11).

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Pumps and Tubings
Picture 7.1.8.
Picture 7.1.9

Rev 20141103
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Pumps and Tubings
Picture 7.1.9
Picture 7.1.10

Rev 20141103
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Pumps and Tubings
Firstly, loosen 4 screws (red circle, Picture 7.1.11) on the metal plate, loosen two
metal srews (red circle, Picture 7.1.11) slowly and gently to remove the tubes, note
do not confuse the position of the two metal srews, the lower one connects to white
starter tube, the upper one connects to black tube. Then you can change your new
pump.
Picture 7.1.11
Note: Since the wrong angle and violent action of loosen or tighten the metal
srews will damage the plastic connector which will lead leakage of the
pump and difficult to repair, so it is very important to be slowly and gently.
Case 1："Starter_1 inject abnormally. Please shut down the analyzer and check carefully!"
Problem：
⑴ The cable of starter pump was loosen or .
⑵ The cable of sensor was loosen or damaged.
⑶ The motor is damaged.
Solution：
⑴ Check whether the cable of starter pump is loosen, and plug in the cable.

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Pumps and Tubings
Picture 7.1.12
Make sure START1 PUMP cable,START1 PUMP cable,START1 SENSOR cable and
START2 SENSOR cable are plugged in the right places as shown in Picture 7.1.12.
⑵ Check whether the cable of sensor was loosen, and plug in the cable.
⑶ Change the motor.
Case 2：Suck-back of starter pump, lead to the first assay results lower.
Problem：
⑴ Gas leakage of starter pump.
Solution：
⑴ Check whether there is suck-back in the starter sprayer,if it is,fasten the
metal srews of in-let pipe and out-let pipe.
Case 3: Noise from starter pump.
Problem：
⑴ The piston bar was worn.
Solution：
⑴ Check whether there is a liquid leakage of the starter pump, or after adjusting
the pump volume, the volume become inaccurate in a short time. If it is, change
the piston bar.

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Pumps and Tubings
Case 4: Starter tube was damaged.
Problem：
⑴ When the starter tube was damaged, the volume will become inaccurate,and
will get lower RLU, therefor cause bad results.
Solution：
⑴ Check the starter tube and change the damaged tube.
7.1.2.4 Volume adjustment of starter pump

For some cases, we doubt about the volume of starter pump 1/2,in these cases,
we need to check whether the volume is inaccurate first, if it is, then we need to
adjust it.
⑴Turn off the analyzer,unplug the cable of PMT (Cable 1, Picture 7.1.13) and take
off the chamber cover, then put a cuvette at the bottom of starter spray (Picture
7.1.14).
Picture 7.1.13
Picture 7.1.14

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Pumps and Tubings
⑵Open the service software and initialize, after that click “pumps” (Picture 7.1.15).
Picture 7.1.15
⑶Click “starter 1” to check volume of starter pump 1 (“starter 2” for starter pump 2).
Click this buttom 2 times, so the pump will inject for 2 times, then there will be
400μL in the cuvette.（please reference to the 400μL line of cuvette）. If the
volume is less/more than 400μL, you need to adjust the volume according to the
following method:

Rev 20141103
Maglumi® Series
Pumps and Tubings
⑷ If the volume is less than 400μL, first loosen the metal screw (red circle, Picture
7.1.16), and move Part A down to reduce the acute angle to increase the volume.
If the volume is higher than 400μL, first loosen the metal screw (red circle,Picture
10), and move Part A up to enlarge the acute angle to decrease the volume.
Picture 7.1.16
7.1.3 Vacuum bypass pump

7.1.3.1 Introduce
The vacuum bypass pump is a gas-liquid transfer pump. The use of a special
diaphragm allows the pump to transfer both air and liquid. In Maglumi analyzer it is
responsible for transporting system liquid to diluter pump, thus to wash the needle.
The following pictures are out-side view (Picture 7.1.17) and internal structure
(Picture 7.1.18) of this pump. Along with the diaphragm move back and forth driven
by the motor, the system liquid trans“In” and “Out”.

Rev 20141103
Maglumi® Series
Pumps and Tubings
1 -- Pump Head
2 -- Valve
3 -- Valve seat
4 -- Diaphragm
5 --Sealing
gasket
Picture 7.1.17 Picture 7.1.18

Firstly, loosen two screws (red circle, Picture 7.1.19) on the base, and loosen
two metal clips (red circle, Picture 7.1.19), then easliy remove the two tubes (Picture
7.1.20), upluge the corresponding cable on the backpanel.And change your new
pump.
Picture 7.1.19

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Maglumi® Series
Pumps and Tubings
Picture 7.1.20
Phenomenon：No liquid when needle is washing.

Problem：
⑴ Vacuum bypass pump do not work.
⑵ Pipeline/pump is blocked.
Solution：
⑴ Check the cable of vacuum bypass pump and make sure it is OK. Turn on the
machine and go to service software-->click -->click to run the

bypass pump, to hear the voice and see the movement of the punp, if there is not
any voice or movement, it means that the bypass pump is damaged.
⑵ Check whether the pipeline/pump is blocked, and dredge the pipeline. Turn on the
machine and go to service software-->click -->click to run the

bypass pump. To see if there is a water flow from the inlet pipe to the pump then to
the outlet pipe, if there isn’t, it means that the pipeline/pump is blocked. Sometimes
system luquid may produce some crystals at two valves (red circle, Picture 7.1.22) so
block the pump. Here

Rev 20141103
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Pumps and Tubings
Picture 7.1.21
Picture 7.1.22
7.1.4 Condensate Water Pump
A. Peristaltic Pump
7.1.4.1 Disassembling of the condensate water pump (Peristaltic

pump)
To disassemble the peristaltic pump for condensate water, we need to loosen two
pieces of screws and disconnect two connectors for tubing and one power cables for
the motor

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Pumps and Tubings
Picture 7.1.23
Picture 7.1.24

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Pumps and Tubings
7.1.4.2 Overview of pump for consendate water (Old design-
peristaltic pump)
Picture 7.1.25
1. Motor
2. Power cable for
motor
3. Peristaltic pump

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Pumps and Tubings
7.1.4.3 Structure of the peristaltic pump
Picture 7.1.26
Picture 7.1.27

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Pumps and Tubings
7.1.4.4 Maintenance to the peristaltic pump
Normally, the tubing inside the peristaltic pump should be replaced every 18-24
months. If working under bigger load, it should be more often.
Picture 7.1.28
Case 1 “Condensate water cannot be removed from reagent area“
(1) Please enter service software to check whether the pump can work when clicking
[Condensate].

Rev 20141103
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Pumps and Tubings
Picture 7.1.29
(2) If the pump does not respond at all, when clicking [Condensate], please measure
working voltage of the pump.
Picture 7.1.29
---If the working voltage is 14-27V-DC, please replace the pump.
---If the working voltage is out of range, you need to replace the 06-E01 interface
board or 06-E00 PCB board

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Maglumi® Series
Pumps and Tubings
(3) If the pump can work but no water flow through the tubing, you need to replace
the tubing inside the pump (Please refer to Maintenance to the peristaltic pump).
(4) Enter service software to check whether the peristaltic pump is right set. We can
adjust the Condensate Time to change how long the pump work for one round,
and adjust the Condensate Int. to change the interval. The default setting is as
follow, but you can adjust it according to environment.
Picture 7.1.30
B. New design for concentrate water pump
Unlike the old design, the pump is replaced by diaphragm pump. We still need to
disconnect the tubing and power supply cable, but loosen one screw only.

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Pumps and Tubings
Picture 7.1.31

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Pumps and Tubings
7.2 Tubing System

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Pumps and Tubings

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Appendix Maglumi® 600/800
Appendix
Chapter 8
Appendix
8.1 COORDINATES M600 ......................................................................................... 3

8.1.1 PREPARATION FOR COORDINATE ....................................................... 3
8.1.3 ADJUSTMENT OF REFERENCE POSITION .......................................... 6
8.1.4.1 ADJUSTMENT OF< FIRST SAMPLING> POSITION ....................................... 9
8.1.4.2ADJUSTMENT OF SECOND SAMPLING AFTER INCUBATE ........................... 12
8.1.4.3 ADJUSTMENT OF SECOND SAMPLING AFTER WASH ................................ 15
8.1.5 WASHING POSITION ADJUST .............................................................. 20
8.1.6 SAMPLE REAGENT AREA POSITION ADJUST ................................... 22
8.1.6.1 SAMPLE AREA POSITION ADJUST ........................................................... 23
8.1.6.2 REAGENT AREA POSITION ADJUST ........................................................ 27
8.1.7 Z-DISPENSE POSITION ADJUST ....................................................................... 33
8.1.8 ADJUSTMENT OF THE PIPETTING NEEDLE IN Z- START POSITION 37
8.2 COORDINATES M800 ....................................................................................... 39
8.2.1 SAMPLE ARM/PIPETTES....................................................................................... 39
8.2.2 PREPARATION FOR COORDINATE.................................................................. 40
8.2.3 PROCEDURE OF COORDINATES ..................................................................... 43
8.2.4 ADJUSTMENT OF REFERENCE POSITION ................................................... 44
8.2.5 ADJUSTMENT OF PIPETTING NEEDLE .......................................................... 46
8.2.6 ADJUSTMENT OF <FIRST SAMPLING> POSITION .................................... 46
8.2.7 ADJUSTMENT OF SECOND SAMPLING AFTER INCUBATE ................... 48
8.2.8 ADJUSTMENT OF SECOND SAMPLING AFTER WASH............................ 49

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Appendix
8.2.9 ADJUST OF THE MIX POSITION IN THE SECOND SAMPLING AFTER
WASH ....................................................................................................................................... 51
8.2.10 WASHING POSITION ADJUST .......................................................................... 52
8.2.12 REAGENT AREA POSITION ADJUST ............................................................. 57
8.2.13 Z-DIEPENSE POSITION ADJUST ..................................................................... 60
8.2.14 ADJUSTMENT OF THE PIPETTING NEEDLE IN Z-START POSITION
.................................................................................................................................................... 62
8.2.15 ADJUSTMENT OF DATA COMPESATION .................................................... 62
8.3 SOFTWARE UPGRADE .................................................................................... 66
8.3.1.1 KERNEL OR STRUCTURE OF OPERATING SOFTWARE UPGRADE ........................ 66
8.3.1.2 OPERATING SOFTWARE PATCH UPDATE .................................................................. 71
8.3.2 MASTER CONTROL PCB PROGRAMS’ UPGRADE .................................... 72
8.3.2.1M600-01-E00-COP OR M800-01-E00-COPWRITE THE FLASH OUT OF THE
UPGRADE PROGRAM ....................................................................................................................... 73
8.3.2.2 M600- 02～04 BOARD OR M800-02～05PROGRAM UPGRADE WRITING
FLASH OUT ......................................................................................................................................... 76

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Appendix
8.1Needle Coordinates
8.1 Coordinates M600

After installment of the analyzer and computer,the pipettors’ plane
position and the maximum position should be coordinated at first.
8.1.1 Preparation for coordinate

Using two reaction modules fill 75μl of water and then these two
cuvettes respectively place to first slot of incubator and one position
in front of the right pipetting position
For Maglumi600,using three tubes filled with100μl of water should be

respectively placed at the track#1,1st and 8th position sample area
in,track#2, 8th position in sample area.
For Maglumi800, using threetubesfilled with100μl of water should be

respectively placed at the track#1, 1st and 8th position sample area in,
track#5,8th position in sample area.
Fill the 1st vial (the position of magnetic particle) of the reagent kit
used toCoordinate with 200μl water, while the 2nd vial (the position of
low calibrator) with 200μl water, the 4th vial (the position of
displacer)with 500μl water and the 7th vial (the position of diluter)with
1000μl water.
Then slide the reagent kit into the track#1 of Reagent Area.
Switch on to start the analyzer. Turn on the PC system. Double click

the [Maglumi Service] icon on the desktop, if the connection between
the PC and the analyzer is OK,entered password (snibe923),the
[Maglumi Service]Interface will be shown as below Fig 8.1-1

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Appendix
Fig. 8.1-1: [MAGLUMI Service]dialog
Press<Initialize>button on the top center of the dialog to execute

initialization of the planeassemblies.
When the initialization finishes, press<Incubator>buttonto open the
[Incubator] dialog.Select in the [target position], click
icon, back to the [Maglumi Service]interface . Click
button to open[Sample Loader] dialog。

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Appendix
Fig. 1.1-2:[Sample Loader]dialog
Click<both> icon, one reaction module will be moved to thefirst

pipetting position
Click <OK> icon, back to the [Maglumi Service] dialog
8.1.2 Procedures of Coordinates

Click the upper left corner button of [Maglumi 600 Service] interface to open[Needles
Adjust]dialog.
Fig. 1.2-1: [Needles Adjust] dialog

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Maglumi® 600/800
Appendix
8.1.3 Adjustment of reference position
Click button onupper right corner of the [Needles
Adjust]interface to open[Ref. Adjust]dialog.
Fig. 1.3-1: [Ref. Adjust] dialog
Select<Motion Track1>In the field of [Select Motion

Track ]of reference position.Toset the stepsize-bar can adjust value
of the stepsize.Set bigger stepsizefirstly, then press the icon to
move the pipettor needle downward. When the needle gets close to
the target posision, set stepsize smaller, and then click the Clockwise
directionicon of outside arm,click the Counterclockwise
direction icon of outside arm, click the Clockwise direction
of inside arm, click the couterclockwise direction of inside arm.

making the needletip just above the center point of reference
position, Then press and icon to make the needletip

higher than the reference position center point by 0.5mm. Save the
parameters with icon, then press <OK>toexit.

And then adjust the Motion Track2 as the way of Motion
Track1, click to exit the interface.
NOTE: Ensure that Motion Track1and Motion Track2 adjust the same
reference position.

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Appendix
Requirement:
1. Needle tip should be located in the center point of thereference position,which is located
on the protection board of reagent area.
Reference Position
2. The Needletip higher than the reference position center point by 0.5mm
After adjust the pipetting needle, click icon to exit the interface.

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Appendix
8.1.4 Adjustment of pipetting needle
Click the icon in the right side of[Needles

Adjust]interface to open the[RightPipetting Position
Adjust]dialog
Fig. 1.4-1: [Right Pipetting Position Adjust]dialo

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Appendix
8.1.4.1 Adjustment of< First Sampling> position
Select First Sampling in the field of [Select Sampling Time]

Select the Position of 1 in the field of [Select Area].
Fig. 1.4.1-1: [Right Pipetting Position Adjust](First Sampling,Position of 1 )
Toset the stepsize-bar can adjust value of the stepsize.Set bigger
stepsizefirstly,click the icon to downward the pipettor. When the

pipettorget close to thereaction module ,set stepsize smaller. Then
click the Clockwise direction icon of outside arm,click the
Counterclockwise direction iconof outside arm, click the Clockwise
direction icon of inside arm, click the couterclockwise direction
icon of inside arm. Make the needletip just above the target
position, click upward icon, downward icon to make the

needle tip reaches the Zmax in module.Save the parameters with
icon.

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Appendix
Requirements:
1. Plane position: The edge of the needle should be aligned
with the corner of the reaction modulecavity.
2. Zmax in module: click and icon to adjust the pipettor

needle. When the needle tip reaches the liquid level, the LLD’light
would be on, then press icon to save the parameters.
Select Position of 6 in[Select Area]

Fig. 1.4.1-2: [Right Pipetting Position Adjust] (First Sampling, Position of 6 )
stepsizefirstly,click the icon to downward the pipettor. when the

pipettor get close to thereaction module ,set stepsize smaller. Then
Counterclockwise direction icon of outside arm, click the
Clockwise direction icon of inside arm, click the couterclockwise

Rev 20141103
Appendix
direction icon of inside arm. Make the needletip just above the
target position, click upward icon, downward icon to make

the needle tip reaches the Zmax in module.Save the parameters with
icon.
Requirements:
1.Plane position: The edge of the needle should be alignedwith the
corner of the reaction modulecavity.


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Appendix
8.1.4.2 Adjustment of Second Sampling after Incubate
SelectSecond Sampling after Incubatein the field of

[Select Sampling Time]
select the Position of 1 in the field of [Select Area]
Fig. 1.4.2-1: [Right Pipetting Position Adjust](Second Sampling after Incubate, Position of 1 )


icon.
Requirements:

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Appendix

Select thePosition of 6in the field of [Select Area]
Fig. 1.4.2-2: [Right Pipetting Position Adjust](Second Sampling after Incubate, Position of 6 )


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Maglumi® 600/800
Appendix

icon.
Requirements:


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Appendix
8.1.4.3 Adjustment of Second Sampling after Wash
Back to [Maglumi 600 Service] interface.
Click icon to open[Incubator]dialog. Select Backtransport

Position in the field of [Target Position], click icon, back
to[Maglumi Service]dialog
Press icon in[Maglumi 600 Service]interfaceto open[Back

Transport]dialog
Fig. 1.4.3-1: [Back Transport] dialog
Click<Move>icon, transport 1 reaction module to the position of

Second Sampling after Wash, and push one cuvette back toSlot 1 of
Incubator. Click icon, back to[Maglumi Service]dialog
Select Second Sampling after Wash in [Select Sampling Time] interface

Select Position 1 in [Select Area]

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Maglumi® 600/800
Appendix
Fig. 1.4.3-2: [Right Pipetting Position Adjust](Second Sampling after Wash, Position 1)


icon.
Requirements:
1.Plane : The edge of the needle shouldbe approximately1-2 mm
away from the back wall& centered to the forwardcorner.

Rev 20141103
Appendix
2. Zmax in module: click to open to open 、 icon to

adjust the pipettor needle. When the needle tip reaches the
liquid level, the LLD’light would be on, then press

icon to save the parameters.
Select Position of 6 in[Select Area]
Fig. 1.4.3-3: [Right Pipetting Position Adjust](Second Sampling after Wash, Position 6)


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Maglumi® 600/800
Appendix

icon.
Requirements:
1.Plane : The edge of the needle shouldbe approximately1-2 mm
away from the back wall& centered to the forwardcorner.
2. Zmax in module: click 、 icon to adjust the pipettor

needle. When the needle tip reaches the liquid level, the
LLD’light would be on, then press icon to save the

parameters.
Select Position of Mix in [Select Area]

Fig. 1.4.3-4: [Right Pipetting Position Adjust](Second Sampling after Wash, Position of Mix )

Rev 20141103
Appendix
Then click the Clockwise direction icon of outside arm,click the
direction icon of inside arm, adjust the Plane Position of Pipetting

Needle.
Requirements: The needle should becentered to the forwardcorner.

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Maglumi® 600/800
6 0
Appen
ndix
8.1.5 Washin
ng position adjjust
C
Click the wasshing positio
on icon in the [Nee
edles Adjus
st]
dialog to ope
d en the [Washing Positio
on Adjust] d
dialog.
g.1.5 : [Wash
Fig hing Positio
on Adjust]dia
alog
Select [Was
ste Position] or [Prime Position]
P in [Select Area
a],
T
Toset the ste
epsize-bar ca
an adjust valu epsize.Set bigger
ue of the ste
sstepsizefirstlyy, then press

s the iccon to move tthe pipettor needle
n
d
downward. W
When the nee
edle gets close to waste //prime positioon, set
s
stepsize aller. Click the Clockwise direction
sma icon of outtside
a
arm,click the Counterclocckwise directiion icon of outside arm,
a click
the Clockwise
e direction icon of in
nside arm, cliick the
ccouterclockwwise direction icon off inside arm, adjust the Plane

P
P
Position of Pipetting Needdle.
Making the needletip jusst above the waste /prime e position. Th
hen
p
press a
and icon until the ne
eedle reache
es the target position.
S
Savethe para
ameters with
h icon.
R
Requiremen
nts:
1 . The nee
edle of the waste
w posittionmust alw
ways be hig
gher than
Page 8-20
8 S
Service Manual V#2.1
Rev 2014110
03
Appendix
theprime position wall. If the wasteposition is lower, the needle
maycrash when moving from thewaste to the prime position.
2.The needle of the Prime Positionshouldbe taught to the bottom
ofthe priming well and 3steps up.

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Maglumi® 600/800
Appendix
8.1.6Sample Reagent area position adjust
Click the icon in the [Needles Adjust] dialog to open

the [Sample Area Reagent Adjust] dialog
Fig. 1.6-1: [Sample Reagent Area Adjust]diallog

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Appendix
8.1.6.1 Sample area position adjust
SelectPosition of Track1 and Tube 1 in[SelectSample Area]

Fig. 1.6.1-1: [Sample Reagent Area Adjust]( Position of Track 1 and Tube 1)


icon.

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Maglumi® 600/800
Appendix
Reauirements:
1.The tube should be filled with 100μl water at first.
2.Plane position: needle’s position should be just at the center point
of the tubemouth.
3.Zmax in module: click and icon to adjust the pipettor


4.Press the <Zmax>icon while holding the "Shift" key on the keyboard,
then the pipettor needlewill movehalf the distance to Zmax.
According to this method, the needle will gradually approach the
Zmax in order to prevent the needle hitting the bottom of the tube.
SelectPosition of Track1 and Tube 8 in [SelectSample Area]


Clockwise direction icon of inside arm, click the
couterclockwisedirection icon of inside arm. Make the needletip
just above the target position, click upward icon, downward

Rev 20141103
Appendix
icon to make the needle tip reaches the Zmax in module.Save the
parameters with icon.
Reauirements:
of the tubemouth.


SelectPosition of Track2and Tube 8 in [SelectSample Area]


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Maglumi® 600/800
Appendix


icon.
Reauirements:
of the tubemouth.



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Appendix
8.1.6.2 Reagent area position Adjust
SelectPosition of Track1 and Tube 1 in[Select Reagent Area]



icon.
Reauirements:
1.Fill 200μl water into the 1st vial (the position of magnetic particle) of
the reagent kit which is used for Coordinate.

of the sealing flap.

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Maglumi® 600/800
Appendix

would be on, then click icon to save the parameters.

SelectPosition of Track1 and Tube 2in [Select Reagent Area]



icon.

Rev 20141103
Appendix
Reauirements:
1.Fill 200μl water into the 2nd vial (the position of low calibrator) of
the reagent kit which is used for Coordinate.






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Maglumi® 600/800
Appendix

icon.
Reauirements:
1.Fill 500μl water into the 4th vial (the position of displacer) of the
reagent kit which is used for Coordinate.





Rev 20141103
Appendix

icon.
Reauirements:
1.Fill 1000μl water into the 7th vial (the position of diluter) of the


Zmax in order to prevent the needle hitting the bottom of the tube
SelectPosition of Track 4 and Tube 7in [Select Reagent Area]


Rev 20141103
Maglumi® 600/800
Appendix


icon.
Reauirements:
1.Fill 1000μl water into the 7th vial (the position of diluter) of the


Zmax in order to prevent the needle hitting the bottom of the tube
5. When the adjustment finishes, take out of the reagent kit which
is used for coordinate and then slide it into the fifteenth track of
Reagent Area

Rev 20141103
Appendix
8.1.7 Z-Dispense position adjust
Fill 600μl water into module’s position 1 and then insert it in the the
slot 1 of Incubator position.
1.Click icon in [maglumi 600 service] interface, after
finished initialize. Click iconto open [Incubator] dialog.

Select Backtransport Position in the field of [Target Position]
（Fig.1.7-2）, and then click icon, back to[Maglumi
Service]dialog
2.Click iconto open [Sample Loader] dialog. Select Both in

the field of [Target Position]（Fig.1.7-3）, and then click
icon, back to[Maglumi Service]dialog
3.Click the upper left corner button of [Maglumi 600
Service] interface to open[Needles Adjust]dialog, click

icon to open Dispense Adjust]dialog （Fig.1.7-4）
Fig .1.7-1 Liquid level of the ReactionModule

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Maglumi® 600/800
Appendix
Fig.1.7-2: [Incubator] dialog
Fig.1.7-3 :[Sample Loader]dialog

Rev 20141103
Appendix
Fig .1.7-4： [Z-Dispense Adjust]dialog
Select Needle in [Select Needle]
Fig .1.7-5：Select pipetting needle in [Z-Dispense Adjust]dialog

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Maglumi® 600/800
Appendix

pipettor get close to thereaction module ,set stepsize smaller. Click
and icon to adjust the pipettor needle. When the needle tip
reaches the liquid level, the LLD’light would be on, then click
icon to save the parameters.

Rev 20141103
Appendix
8.1.8 Adjustment of the pipetting needle in Z- Start position
Click icon to open[Z-StartAdjust]dialog
Fig. 1.8-1: [Z-Start Adjust]dialog.
Fig .1.8-2：Select Pipetting Needle in [Z-Dispense Adjust]dialog

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Appendix
Toset the stepsize-bar can adjust value of the stepsize. Press

icon tomove the pipettor needle downward. When 2/3of the needle tip
coated with Teflon just cross the sealing flap to the 1st vial mouth of
the reagent kit, press icon to save the parameters.
When the coordinates finishes, press the <OK> button in the

[Needles Adjust]dialog to exit.
Remove the tubes, reaction modules and reagent kit used for
coordinate from the analyzer and then transmit a new reaction
module to the left pipetting positon.

Rev 20141103
Appendix
8.2 Needle Coordinates M800
8.2 Coordinates M800

8.2.1 Sample Arm/Pipettes
After installment of the analyzer and computer, it is suggested that the
initialization position of the pipettes must be coordinated, and the
maximum movement of the sample arm have to be examined to
confirm it can cover the whole range of sample and reagent areas.
Please follow the procedure below when coordinating the parameters

of the pipettes.
Note:
1. Please ensure the position of the sample area, reagent area (including the
glass panel) and wash hole fixed before coordinating the position of the
pipette.
2. Please remember to examine the verticality of the pipette, and adjust the
position of the pipette if it is out of plumb before coordinating the position of
the pipette.
3. Keep the position of sample rack and reagent kit stable before
coordinating the position of the pipette.
4. Except for the ‘moving track’ in ‘needle reference position’, the other
position of the outside horizontal arm must be on the left side of inside
horizontal arm when coordinating the pipettes (As showed in picture 1).
5. It is proper for the operators to coordinate the pipettes by one or two steps
in order to confirm precision when coordinating the pipettes.
6. Do not put your hands in the moving areas of sample arm in case some
damage may happen.
7. Pictures in this document are just as the reference and please subject to the
actual adjustment.

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Appendix
Figure 1 Inner arm and outer arm positional relation (top view)
8.2.2 Preparation for coordinate

(1) Take out of two cuvettes from the package, and pour 75μl of water
in 1st and 6th well of one cuvette. And pour 600μl of water in 6 well of
the other cuvette. Put these two cuvettes on the cuvette loader as the
sequence.
(2)Three sample tubes filled with 100μl of water are respectively
placed at the track#1, 1st and 8th position sample area in, track#5, 8th
position in sample area.
(3) Fill the 1st vial (the position of magnetic particle) of the reagent kit
used to Coordinate with 200μl water, while the 2nd vial (the position of
low calibrator) with 200μl water, the 4th vial (the position of displacer)
with 500μl water and the 7th vial (the position of diluter)with 1000μl
water. Then slide the reagent kit into the track#9 of Reagent Area.
(4) Switch on to start the analyzer. Turn on the PC system.
(5) Double clickthe [Maglumi Service] icon on the desktop, if the
connection between the PC and the analyzer is OK,entered password
(snibe923), the [Maglumi Service] Interface will be shown as below
Figure 2.

Rev 20141103
Appendix
Figure 1 [MAGLUMI 800 Service] interface
(6)Press <Initialize> button on the top center of the interface to

execute initialization of the plane assemblies
(7)When the initialization finished, press the<Incubator> button to
open the [Incubator] interface. Select [Backtr] in the [Target Position],
click <OK> icon, back to the [Maglumi Service] interface.

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Appendix
Figure 2 MAGLUMI 800 Service] interface
(8) Press <Sample Load> button to open [Sample Loader] interface.

Select [Both Incubator and Cuvette Loader] in the [Target Position] to
push the cuvette from the cuvette loader to first slot of incubator.
Figure 3 [Sample Loader] interface

Rev 20141103
Appendix
(9) Select [Both incubator and Back Trans] in the [Target Position] to
push the cuvette from the incubator into the pipetting position.
Figure 4 Sample Loader] interface
8.2.3 Procedure of coordinates

Click the upper left corner <Coordinates> button of [Maglumi 800
Service] interface to open [Needles Adjust] interface.

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Appendix
Figure 5 [Needles Adjust] interface
Coordinate Hot Key Table

Description Icon Hot Key
Outer Arm Clockwise Move Outer Arm Adjustment →

Outer Arm Anti-clockwise Move Outer Arm Adjustment ←
Inner Arm Clockwise Move Inner Arm Adjustment ↑
Inner Arm Anti-clockwise Move Inner Arm Adjustment ↓
Vertical Upward Move Vertically Adjustment Page Up
Vertical Downward Move Vertically Adjustment Page Down
8.2.4 Adjustment of reference position

‘Reference position’ situated at the middle of right 7 column, 6 to 7
hole in the right bottom corner of the glass panel.
Click <Ref.Pos> button on upper right corner of the [Needles Adjust]
interface to open [Ref.Adjust] interface.

Rev 20141103
Appendix
Figure 6 [Ref. Adjust] interface
Select <Motion Trace1> in the field of [Select MotionTrace of Ref

Pos].To set the stepsize-bar can adjustvalue of the stepsize. Set
bigger stepsize firstly, then press the icon to move the

pipettor needle downward. When the needle getsclose to the target
posision, set stepsize smaller, and then click theClockwise direction
icon of outside arm, click theCounterclockwise direction icon
of outside arm, click theClockwise direction of
inside arm, click the counterclockwisedirection of inside arm.

Making the needle tip just above thecenter point of reference position,
Then press and icon to make the needle tip higher

than the reference position centerpoint by 0.5mm. Save the
parameters with icon, thenpress <OK> to exit.

And then adjust the Motion Track 2 as the way of MotionTrack1, click
to exit the interface.
Requirement:
1.Needle tip should be located in the center point of the reference
position,which is locatedon the protection board of reagent area.

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Appendix
2.The Needle tip is just higher than the reference position center point
by 0.5mmAfter adjust the pipetting needle, click icon to exit
the interface.
Note:
1. Make sure the position between the MotionTrace 1 and MotionTrace 2 is
the same.
2. The position correlation between the two horizontal arms in ‘MotionTrace
1’: Outside Arm located on the left side of the Inner Arm extension line
3. The position correlation between the two horizontal arms in ‘Motion
Trace 2’: Outside Arm located on the rightside of the Inner Armextension
line.
8.2.5 Adjustment of pipetting needle
Click the icon in the right side of

[NeedlesAdjust]interface to open the [Right Pipetting
PositionAdjust]interface.
Figure 7 [Right Pipetting Position Adjust] interface
8.2.6 Adjustment of <First Sampling> position

Select [First Sampling] in the field of [Select Sampling Time]. Select
the Position of 1 in the field of [Select Area].

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Appendix
Figure 8 [Right Pipetting Position Adjust] interface First Sampling-Position of 1
To set the stepsize-bar can adjust value of the stepsize. Set
biggerstepsize firstly,click the icon to downward the pipettor.

When thepipettor get close to the reaction module , set stepsize
smaller. Thenclick the Clockwise direction icon of outside

arm, click theCounterclockwise direction icon of outside arm, click
theClockwise direction icon of inside arm , click the
counterclockwisedirection icon of inside arm. Make the
needle tip just above thetarget position, click upward icon,
downward icon to makethe needle tip reaches the Z-max in
module. Save the parameters with icon.
Requirement:
Plane position: The edge of the needle should be alignedwith the
corner of the reaction module cavity.

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Maglumi® 600/800
Appendix
Z-max in module: click and icon to adjust the

pipettorneedle. When the needle tip reaches the liquid level, the
LLD’lightwould be on, then press icon to save the parameters.
8.2.7 Adjustment of Second Sampling after Incubate

Select [Second Sampling after Incub] in the field of[Select Sampling
Time],select the Position of 1 in the field of [Select Area].
Figure 9 [Right Pipetting Position Adjust] interface Second Sampling after Incub-Position of 1

When thepipettor get close to the reaction module, set stepsize


Rev 20141103
Appendix
theClockwise direction icon of inside arm, click the
counterclockwisedirection icon of inside arm. Make the
module. Save the parameters with icon.
Requirement:
Plane position: The edge of the needle should be alignedwith the
corner of the reaction module cavity.
Z-max in module: click and icon to adjust the

pipettorneedle. When the needle tip reaches the liquid level, the LLD’
lightwould be on, then press icon to save the parameters.
8.2.8 Adjustment of Second Sampling after Wash

Note: Firstly, cuvette must be put in the back transport position, and
then pushed into the Second Sampling position.
Select [Second Sampling after Wash] in [Select Sampling Time]
interfaceSelect Position 1 in [Select Area].

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Maglumi® 600/800
Appendix
Figure 10[Right Pipetting Position Adjust] interface Second Sampling after Wash-Position of 1

When thepipettor get close to the reaction module, set stepsizesmaller.
Thenclick the Clockwise direction icon of outside arm, click

theCounterclockwise direction icon of outside arm, click theClockwise
direction icon of inside arm, click the
Counterclockwisedirection icon of inside arm. Make the
module. Save the parameters with icon. Apply the

same method to adjust the position 6 in[Second Sampling after Wash].
Requirements:

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Appendix
1. Plane: The edge of the needle should be approximately 1-2 mm
away from the back wall & centered to the forward corner.
2. Z-max in module: click to open to open ﹑ icon to

adjust the pipettor needle. When the needle tip reaches theliquid level,
the LLD’ light would be on, then press icon to save

the parameters.
8.2.9 Adjust of the mix position in the second sampling

after wash
Figure 11[Right Pipetting Position Adjust] interface Second Sampling after Wash-Position of Mix

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Maglumi® 600/800
Appendix
Then click the Clockwise direction icon of outside arm, click
the Counterclockwise direction icon of outside arm, click the
Clockwise direction icon of inside arm , click
thecounterclockwisedirection icon of inside arm, adjust the

Plane Position ofPipetting Needle.
Requirements: The needle should be centered to the forward corner.
8.2.10 Washing position adjust
Click the wahing position icon in the [Needles adjust]

interface to open the [Washing Position Adjust] interface.
Figure 12[Washing Position Adjust] interface

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Appendix
Select [Waste Position] or [Prime Position] in [Select Area], to set the
stepsize-bar can adjust value of the stepsize. Set bigger stepsize
firstly, then press the icon to move the pipettor needle

downward. When the needle gets close to waste/Prime position, set
stepsizesamller. Click the Clockwise direction icon of
outside arm, click the counterclockwise direction icon of
outside arm, click the clockwise direction icon of the inside
arm, click the counterclockwise direction icon of inside arm,

adjust the plane Position of Pipetting Needle. Making the needle tip
just above the waste/prime position. Then press and
icon until the needle reaches the target position. Save the
parameters with icon.
Requirements:
1. The needle of the Waste Position must always be higher than the
prime position wall. If the waste position is lower, the needle may
crash when moving from the waste to the prime position.
2. The needle of the Prime Position should be taught to the bottom of
the priming well and 3 steps up.

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Maglumi® 600/800
Appendix
8.2.11 Sample area position adjust
Click the icon in the [Needles Adjust] interface to open the

[Sample Area Adjust] interface.

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Appendix
Figure 13[Sample Area Adjust] interface
Select Position of Track 1 and Tube 1 in [Select Sample Area]

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Maglumi® 600/800
Appendix
Figure 14[Sample Area Adjust] interface Position of Trace 1 and Tube 1



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Appendix
module. Save the parameters with icon. Take the

same method to adjust the [Track 1, Tube 8], [Track 5, Tube 8].
Requirements:
1. The tube should be filled with 100μl water at first.
2. Plane position: needle’s position should be just at the center point
of the tube mouth.
3. Z-max in module: click and icon to adjust the

lightwould be on, then press sicon to save the

parameters.
4. Press the <Z-max> icon while holding the "Shift" key on the
keyboard, then the pipettor needle will move half the distance to Z-
max. According to this method, the needle will gradually approach the
Z- max in order to prevent the needle hitting the bottom of the tube.
8.2.12 Reagent area position Adjust
Click the icon in the [Needles Adjust] interface to

open the [Sample Area Adjust] interface.

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Maglumi® 600/800
Appendix
Figure 15[Reagent Area Adjust] interface
Select Position of Track 9and Tube 1 in [Select Reagent Area].

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Appendix
Figure 16[Reagent Area Adjust] interface Position of Track 9 and Tube 1



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Maglumi® 600/800
Appendix
module. Save the parameters with icon. Take the

same method to adjust the [Track 9, Tube 2], [Track 9, Tube 4], [Track
9, Tube 7], [Track 1, Tube 7].
Requirements:
1. Fill 200μl water into the 1st vial (the position of magnetic particle)
ofthe reagent kit which is used for Coordinate.
Fill 200μl water into the 2st vial (low calibration) ofthe reagent kit
which is used for Coordinate.
Fill 500μl water into the 4st vial (displacing reagent) ofthe reagent kit
which is used for Coordinate.
Fill 1000μl water into the 7st vial (dilution) ofthe reagent kit which is
used for Coordinate.
2. Plane position: needle’s position should be just at the center pointof
the sealing flap.
3. Z-max in module: click and icon to adjust the

lightwould be on, then click icon to save the

parameters.
4. Press the <Z-max> icon while holding the "Shift" key on the
keyboard, then the pipettor needle will move half the distance to Z-
max. According to this method, the needle will gradually approach the
Z-max in order to prevent the needle hitting the bottom of the tube.
8.2.13 Z-diepense position adjust

1. Refer to the procedure (7), (8), (9) in Section 2.2 before.
2.Fill 600μl water into module’s position 1 and then push the cuvette
from the cuvette loader into right sampling areas.
3.Click the upper left corner button of

[Maglumi800Service]interface to open [Needles Adjust]interface, click
icon to open [Z-Dispense Adjust]interface.

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Appendix
Figure 17[Z-Dispense Adjust] interface
To set the stepsize-bar can adjust value of the stepsize. Set bigger
stepsizefirstly,click the icon to downward the pipettor. Whenthe
pipettor get close to the reaction module, set stepsize smaller.Click
and icon to adjust the pipettor needle. When theneedle tip reaches the
liquid level, the LLD’ light would be on, thenclick icon to save the
parameters.

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Maglumi® 600/800
Appendix
8.2.14 Adjustment of the pipetting needle in Z-start
position
Click icon to open[Z-Start Adjust] interface,Select Needle
in [Select Needle].
Figure 18[Z-Start Adjust] interface
(1) Inset one reagent kit in reagent area from the right direction.
(2) To set the stepsize-bar can adjust value of the stepsize. Press
icon to move the pipettor needle downward. When 2/3of the

needletip coated with Teflon just cross the sealing flap to the 1st vial
mouthof the reagent kit, press icon to save the

parameters.When the coordinates finishes, press the <OK> button in
the[Needles Adjust] interface to exit.
8.2.15 Adjustment of data compesation

Press the [Detect] icon in the [Needles Adjust] interface to open the
[Detect] interface.

Rev 20141103
Appendix
Figure 19[Detect] interface
(1) Inset one reagent kit in reagent area from the left direction.
(2) Select the [Reagent Area] in [SelectArea], and then click the [Move]
icon to make the pipette move to the tack 1 and 7 hole in reagent area.
To set the stepsize-bar can adjust value of the stepsize. Press
icon to move the pipettor needle downwarduntil it is just

above the sealing flap and observe whether needle tip direct to the
center of the sealing flap.
(3) If the tip directs the center of the sealing membrane, it means that
the amount of the compensation is good, and press the <OK> button
in the[Detect] interface to exit. If not, it means the parameter in
[Compensation] icon need to be adjusted. Press the [Compensation]
icon at the bottom right corner of [Detect] interface to open the [Pipe
Compensate] interface.

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Maglumi® 600/800
Appendix
Figure 20[Pipe Compensate] interface
(4) Change the compensation parameter, and then click the [OK] icon
go back to [Detect] interface. Repeat the procedure (2), (3) until the
needle tip direct to the center of the sealing flap.
The key point of the coordinate and announcement

Preperation:
1. Observe whether the initial position deviate or not;
This position fix on the glass panel, and it is acceptable if the deviation of needle position is
in the range of 10 mm.
The function of this position is providing the initial reference position for engineer when
disassembling the sample arm.
2. Examine the maximum moving position of the needle, and make sure its moving range can
cover the whole range of the sample and reagent area.
3. Make sure whether the position of the sample area, reagent area (glass penal) and wash
hole is fixed.
4. Please remember to examine the verticality of the pipette, and adjust the position of the
pipette if it is out of plumb.
5. Prepare the cuvettes, tube and reagent kit with suitable liquid volume. Push the cuvette into
sampling area, and inset sample rack and reagent lit into the sample and reagent area
respectively. Make sure the position of them stable.
Start coordinate:
As the sample arm of the M800 is too long, which can lead to the large deviation when
needle deviate from setpoint a little, it is advised that to set stepsize 1 or 2 step is acceptable
to ensure the precision when the needle arrive to the targeted position nearly.
LLD’ indicator is on the software interface.
6. Reference position
MotionTrace 1: Outer Arm is on the left side of the InterArm extension line.
MotionTrace 2: OuterArm is on the right side of the InterArm extension line.
7. Sampling Position, Wash Area, Sample Area, Reagent Area

The relative position of the two arms: outside arm is on the left side of the inside arm
extension cord.

Rev 20141103
Appendix
8. As the needle of the M800 is adjusted as the circular motion mode, it can bring the
calculation error if the arm levelness and perpendicularity of the needle is bad. It cannot be
adjusted as the levelness of the arm is controlled by the assembly. And thought the
perpendicularity of the needle is available to be adjusted by 4 method correlation, it
cannotguarantee the precision of movement only by naked eye. The calculation error can be
amplified as the length of the two arm added is too long. So After completing the adjustment
above, M800 need a compensation parameter to debug.
The [Compensation Parameter] interface is on the bottom right of [Needles Adjust]-

>[Detect] interface. Through Testing the position accuracy of reagent 1-1 guarantee whether
the compensation parameter is good or not. Reasons: Firstly, as reagent area is the largest
rectangular in the moving range of sampling arm, the deviation of sample area is most
apparent if the compensation parameter is impropriate. Secondly, it is convenient for us the
make sure the position as there are sealing flap on vial of reagent kit. Thirdly, 9-1,9-7,1-7
position in the reagent area is applied to coordinate the needle position in reagent area, and
the rest position in reagent area are gained according to this three positions. If the needle
position in reagent area is abnormal, the deviation of 1-1 position is the largest. So it is
considered that the compensation is Ok when the position of the 1-1 is accurate.
Finally, Make sure the position of M800 needle in every position is good:
1. Arm levelness is good, perpendicularity of the needle is good.
2. Set the size-step to 1 or 2 step when the needle arrive to the targeted position nearly.
3. Compensation parameter is good.
After crashing the needles:

1. Adjusting the verticality of needle through method 4.
2. Examine the position of ‘Reference position’ to see whether the needle position is good or
not. If not, you should examine the position again to ensure needle movement can cover the
whole range of the sample and reagent area.
3. Through testing the accuracy of the 1-1 position in reagent area to ensure whether the
compensation parameter is appropriate.

Rev 20141103
Maglumi® 600/800
Appendix
8.3 Software Upgrade

8.3.1 OperatingSoftware Upgrade
In order to constantly improve operating
software of MALGUMI series automatic
chemiluminescence analyzer, it is necessary
to upgrade it.
Upgrade modeincludes:
1) Install new version of the operating
software,
2) Upgrade some small patch of software.
This chapter will introduce the general

procedure of software upgrade. We have
the special Guidance document for every
specific software upgrade.
8.3.1.1 Kernel or structure of operating software upgrade
When the Kernelor structure of operating software

changes, the analyzer need to be upgraded to a
new version.
The new version of the operating software will be
sent to you by email or by CD. And BACKUP the
previous operating software.
Upgrade steps:
1)Backup four important folders in“C: program files\Maglumi
600”, including assay, component, report, config
2) Uninstall the old version, theninstall the new
version.
3) Copy the four important folders to cover in the new
version of software.
4) Restart the computer system.
5) Turn on the analyzer, run SERVICE.EXE
software to detect and re-adjust the position of
pipetting needle. After that exit the dialog.
6) Run Priming, BGW and LC to ensure the status
of the analyzer.
7) Suggest that run Two Step Assay
once, confirm the result in target range, unsure
analyzer upgrade successfully.

Rev 20141103
Appendix
The installation process of the specific steps please see pictures below:
Fig .1.1.1-1：MAGLUMI®600 MAGLUMI Users operating software installation process1

Rev 20141103
Maglumi® 600/800
Appendix

Rev 20141103
Appendix
Fig .1.1.1-5：MAGLUMI 600 MAGLUMI Users operating software installation process5
®

Rev 20141103
Maglumi® 600/800
Appendix

Rev 20141103
Appendix
8.3.1.2 Operating software patch update
Sometimes, the system software only need to be

upgraded for a small function or do some small
change, you needn’t uninstall the old version and
install the new version.
You only need to copy the upgrade files and then
cover these files in the “C;/Program
files/Maglumi600”. If there are more steps to do,
it will be mention in the“upgrade instruction”

Rev 20141103
Maglumi® 600/800
Appendix
8.3.2 Master control PCB Programs’ Upgrade
Each component of analyzer has been controlled by
corresponding PCB board. The controlling circuit has
dataline interface for upgrade.
For Maglumi 600,there are 4 pieces of master control

PCB boards on the analyzer can be upgraded, they
control components respectively, their numbers and
names are :
a) M600-01-E00-COP
b) M600-02-E00-Sample&Reagent
c) M600-03-E00-Incubator&Washer&Pusher
d) M600-04-E00-MeasureChamber
PCB board schematic diagram

4 pieces of master control PCB board should be
upgraded by two different methods.
Method 1:M600-01 - E00 - COP can be upgraded

program by the RS232 directly.
Method 2: M600-02 - E00,M600-03-E00 andM600-04-
E00 board should be upgraded by special upgrading
cable.
For Maglumi800,there are 5 pieces of master control PCB

boards on the analyzer can be upgraded, they control
components respectively, their numbers and names are :
a) M800-01-E00-COP
b) M800-02-E00-Sample Arm
c) M800-03-E00-Washer
d) M800-04-E00-MeasureChamber(it is the same as M600-
04-E00-MeasureChamber )
e) M800-05-E00-Multiple Modules
PCB board schematic diagram

5 pieces of master control PCB board should be
upgraded by two different methods.
Method 1:M800-01 - E00 - COP can be upgraded

program by the RS232 directly.
Method 2: M600-02 - E00,M600-03-E00,M600-04-E00
and M600-05-E00 board should be upgraded by special
upgrading cable.

Rev 20141103
Appendix
8.3.2.1M600-01-E00-COP or M800-01-E00-COPwrite the
flash out of the upgrade program
Upgrade Procedure
1) Before upgraded, connect USB Port of

computer to RS232 Interface of analyzer with
RS232 data line
2)Take 01- E00- COP out under shutdown
condition, then connect J2 and J3jumper wire
cap see diagram below:
Fig .1.2.1-1：Positionof 01-E00-COPBoard Jumper
3) Restart the computer and analyzer. Operate Flash Magic

exe on computer. Then select options as follows
Step1-Communications：
step option content meaning
1 Device LPC2468 write the flash out of
chip
2 COMPort COM1 Communication port
3 Baud Rate 115200 write the flash out of
speed
4 Oscillator(MHz) 14.31818 Frequency (fixed)
Step2-Erase：
1 Erase all √ Remove all flash
Flash+Code and codes

Rev 20141103
Maglumi® 600/800
Appendix
Rd Prot
Step3-Hex File：
1 Hex File ….hex select document
document written the flash out
(COP.hex)，
provided bySNIBE
Step4-Option：
1 Verify after √ Proofread after
programming writing flash out
Completing to set the four major steps as above, as shown in the diagram below
Fig .1.2.1-2：Upgrade procedure of MAGLUMI®600/800master control PCB boardM600-01-

E00-COP or M800-01-E00-COP
Press Start button to

execute
Step5-Start!：
Press the “start”button，software will startto writeprogram into the
CMOS chip .
After that, Software will display “finished”at left bottom.

Rev 20141103
Appendix
Fig .1.2.1-3: dialog of finished software upgrade
Step6：
after writing flash out the COP program, make sure to pull jumper wire
cap out (must operate under the consideration of interruption of power
supply of analyzer), can hang on one foot of J2, J3, such as
Disconnect the jumpers after programming
If connect COP jumpers wire cap at the

runtime, maycause the COP programs and
data loss, cannot continue to do the
experiment.

Rev 20141103
Maglumi® 600/800
Appendix
8.3.2.2 M600- 02～04 board or M800-02～05program upgrade writing
flash out
Upgrade procedure:
1） Before upgrade, turn off power supply. Use RS232 interface

dataline provided by SNIBE, program download line connect
(shown as below) to computer COM port and ananlyzer 02-04
master board program download interface, program download
line and connections method as below shown
Fig. 1.2.2-1: MAGLUMI600 ananlyzer r master board 02-04, program download line
Fig. 1.2.2-2: MAGLUMI600 ananlyzer master board 02 program download interface: J4

Rev 20141103
Appendix
Fig. 1.2.2-3: MAGLUMI600 ananlyzer master board 03program download interface: J4
Fig. 1.2.2-4: MAGLUMI600 ananlyzer master board 04program download interface: J7

Rev 20141103
Maglumi® 600/800
Appendix
Note: The M800-04-E00 PCB update procedure is the same as M600-04-E00

PCB.Please refer to Fig 1.2.2-4.

Rev 20141103
Appendix
Fig. 1.2.2-8: MAGLUMI600/800 ananlyzer master board program download connections method

Rev 20141103
Maglumi® 600/800
Appendix
RS232 connection
bl
connections method schematic diagram
2) After connect line, start the computer and analyzer. Operate

FLASHLOAD EXE.program on computer , select options as
follows
Step1-Communications：
1 Device write the flash
out of chip
2 COMPort COM1 Communication
port
3 Baud Rate 115200 write the flash
out of speed
4 Oscillator(MHz) 14.31818 Frequency
(fixed)
Analyzer PCB Device#

Maglumi 600 M600-02-E00-Sample&Reagent LPC2387
M600-03-E00-Incubator&Washer&Pusher LPC2387
M600-04-E00-MeasureChamber LPC2132
Maglumi 800 M800-02-E00-Sample Arm LPC2387
M800-03-E00-Washer LPC2132
M800-04-E00-MeasureChamber LPC2312
M800-05-E00-Multiple Modules LPC2468
Step2-Erase：
1 Erase all √ Remove all flash
Flash+Code and codes
Rd Prot
Step3-Hex File：
1 Hex File ….hex select document
document written the flash
out，These 3
master control
boards have 3
different files
respectively, the
file names are

Rev 20141103
Appendix
corresponding to
circuit names,
provided bySNIBE
Step4-Option：
1 Verify after √ Proofread after
programming writing flash out
Completing to set the four major steps as above, as shown in the diagram below
Fig. 1.2.2-6: Procedure of MAGLUMI600/800 ananlyzer master board02-05program upgrade
Step5-Start!：
Press the “start”button，software will startto writeprogram into the
CMOS chip .
After that, Software will display “finished”at left bottom.
Step6:
1) After writing flash out the program, exit Flash
Download.EXEprogram
2) Switch off analyzer, separate data line, reconnect RS232 data line
with analyzer’s RS232 interface
Upgrading program must be followe the principles, not

charged when plug and pull out connecting lines, must
turn off power supply when plug and pull out connecting lines,
otherwise you'll burn master control circuit

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Maglumi® 600/800
Appendix

Rev 20141103

Maglumi Manuel Utilisation

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MAGLUMI® 600

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or misuse of the contained information, be it negligence or

MAGLUMI® Series Service Manual 2.1 - Revision 01

Snibe Co.,Ltd All rights reserved

Service Manual V#2.1 Page 1-1

Page 1-2 Service Manual V#2.1

Service Manual V#2.1 Page 1-3

Page 1-4 Service Manual V#2.1

Service Manual V#2.1 Page 1-5

Page 1-6 Service Manual V#2.1

Service Manual V#2.1 Page 1-1

MAGLUMI 600 / MAGLUMI 800

This document is intended for Field Service Engineers (FSEs) for

1.1 Intended use

This software, which was specifically designed for this purpose,

Any changes to the instrument that are not authorized by the

Page 1-2 Service Manual V#2.1

1.2 Typographical Conventions

1.2.1 Display of Warnings and Notes

The signal word "Caution" and a relating symbol point to potential

The signal word "Caution" point to potential problems.

The signal word "Note" point to potential problems.

Used Warning Symbols

Caution, risk of danger to person or damage to equipment!

Service Manual V#2.1 Page 1-3

Caution, risk of danger to person or damage to equipment!

Page 1-4 Service Manual V#2.1

Service Manual V#2.1 Page 2-1

2.1 System Overview

Page 2-2 Service Manual V#2.1

1. Pipettors 2. Incubator 3. Sample loader

Service Manual V#2.1 Page 2-3

1.Cuvette Loader 2. Sample loader 3. Sample Area

Page 2-4 Service Manual V#2.1

2.1.3 Reaction module process path overview

Wash Meas. Waste

Service Manual V#2.1 Page 2-5

2.2 Liquid Connection

Page 2-6 Service Manual V#2.1

Service Manual V#2.1 Page 2-7

Page 2-8 Service Manual V#2.1

We have 3 dominant ways for signal transmission:

2) Power supply network of M600

Service Manual V#2.1 Page 2-9

Page 2-10 Service Manual V#2.1

1. M600-01-E00 PCB board

Service Manual V#2.1 Page 2-11

Page 2-12 Service Manual V#2.1

1. M800-01-E00 PCB-COP Board

Service Manual V#2.1 Page 2-13

5, M800-04-E00 PCB-Multiple Module

Page 2-14 Service Manual V#2.1

Service Manual V#2.1 Page 3-1

3. Installation of the System

3.2 Analyzer Received

3.3 Unpack and Install the Analyzer

Page 3-2 Service Manual V#2.1

(3) Remove the plastic membrane.

Service Manual V#2.1 Page 3-3

(5) Unscrew the panel.

Page 3-4 Service Manual V#2.1