ScienceDirect - International Journal of Food Microbiology : Comparison of four routine methods for t... http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T7K-409VGKY-H&_user=10&_co...

Hub ScienceDirect Scopus SciTopics Applications Register Login Go to SciVal Suite

Home Browse Search My settings My alerts Shopping cart Help

All fields Author Advanced search

Journal/Book title Volume Issue Page Search tips

Rerun your search for "confirm technique using the Lactose Sulfite medium " on ScienceDirect. Search Font Size:

Purchase Export citation

Related Articles
Abstract Article Figures/Tables References
Rapid reverse CAMP test for identification of Clostridi...
Clinical Microbiology Newsletter
International Journal of Food Microbiology
Tryptose sulphite cycloserine (TSC) agar (without egg y...
Volume 57, Issues 1-2, 10 June 2000, Pages 135-140
Progress in Industrial Microbiology
Investigations and results concerning railway-induced g...
Journal of Sound and Vibration
doi:10.1016/S0168-1605(00)00236-1 | How to Cite or Link Using DOI Selective and differential media for Clostridium perfri...
Copyright © 2000 Elsevier Science B.V. All rights reserved. Cited By in Scopus (5)
International Journal of Food Microbiology
Permissions & Reprints
Tryptose sulphite cycoloserine (TSC) agar (without egg ...
Short communication Progress in Industrial Microbiology
Comparison of four routine methods for the confirmation of View more related articles
Clostridium perfringens in food
,
H. Eisgruber , B. Schalch, B. Sperner and A. Stolle Purchase SciClick
Institute for Hygiene and Technology of Food of Animal Origin, Faculty of Veterinary $ 31.50
Medicine, Ludwig Maximilians University, Munich, Veterinärstr. 13, 80539 Munich,
Free Research Videos

1 of 3 03/21/2011 5:14 PM
ScienceDirect - International Journal of Food Microbiology : Comparison of four routine methods for t... http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T7K-409VGKY-H&_user=10&_co...

Germany
Accepted 17 January 2000. Available online 22 May 2000.
Abstract
In order to compare methods for the confirmation of C. perfringens 52 isolates from strain collections and food
samples were examined using four different techniques including the detection of lactose degradation and sulfite
reduction in lactose sulfite medium (European Standard EN 13401), examination of nitrate reduction, motility,
lactose degradation and gelatine liquefaction in motility–nitrate and lactose–gelatine medium (EN 13401), the
reverse CAMP test (German Standard DIN 10103) and the acid phosphatase reaction according to Ueno et al.
[Ueno, K., Fujii, H., Marui, T., Takahshi, J., Sugitani, T., Ushijima, T., Suzuki, S. 1970. Acid phosphatase in
Clostridium perfringens — A new rapid and simple identification method. Jpn. J. Microbiol. 14, 171–173]. Pure
cultures of the test strains were suspended in sterile physiological saline and decimal dilutions inoculated into
modified SC agar according to Hauschild and Hilsheimer [Hauschild, A.H.W., Hilsheimer, R., 1974. Evaluation and
modifications of media for enumeration of Clostridium perfringens. Appl. Microbiol. 27, 521–526] and Eisgruber
and Reuter [Eisgruber, H., Reuter, G., 1995. A selective medium for the detection and enumeration of mesophilic
sulphite-reducing clostridia in food monitoring programs. Food Res. Internat. 28, 219–226] by pour-plating. Five
individual colonies per test strain (in total 260 colonies) were examined. Using either the reverse CAMP test or the
acid phosphatase reaction 94.2% of the colonies were confirmed as C. perfringens. The detection of nitratase and
motility in combination with lactose and gelatine degradation enabled the identification of C. perfringens in 88.8%
of the colonies. The lowest percentage of C. perfringens colonies was detected via lactose sulfite medium: only
42.7% of the tested colonies showed typical sulfite reduction with gas formation from lactose.
Author Keywords: Clostridium perfringens; Food; EN 13401
Article Outline
1. Introduction

2. Material and methods

2.1. Isolates
2.2. Confirmation via motility–nitrate medium in combination with lactose–gelatine medium
2.3. Confirmation via lactose sulfite medium
2.4. Confirmation via reverse CAMP test
2.5. Confirmation via the acid phosphatase reaction

3. Results and discussion

3.1. Confirmation via motility–nitrate medium in combination with lactose–gelatine medium
3.2. Confirmation via lactose sulfite medium
3.3. Confirmation via reverse CAMP test
3.4. Confirmation via acid phosphatase reaction

References

2 of 3 03/21/2011 5:14 PM
ScienceDirect - International Journal of Food Microbiology : Comparison of four routine methods for t... http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T7K-409VGKY-H&_user=10&_co...

Corresponding author. Tel.: +49-89-2180-3834; fax: +49-89-2180-3872; email: sekretariat@lmhyg.vetmed.uni-

muenchen.de

Purchase
$ 31.50

International Journal of Food Microbiology

Volume 57, Issues 1-2, 10 June 2000, Pages 135-140

Sponsored Links

Sensitive Nitrate detection

Higher Quality, Ultra-Sensitive,
Easy-to-Use & Robust ELISA Kits
www.ArborAssays.com
Search ASTM Lab Directory
Search for Labs by ASTM Standards
ASTM Free Online Lab Directory
www.astm.org/labs
Order Difco microbiology.
Difco agar antigens antiserum
Online ordering ; resale discounts
www.vgdllc.com

Home Browse Search My settings My alerts Shopping cart Help

About ScienceDirect Contact and Support About Elsevier

What is ScienceDirect Contact and Support About Elsevier
Content details About SciVerse
Set up About SciVal
How to use Terms and Conditions
Subscriptions Privacy policy
Developers Information for advertisers

Copyright © 2011 Elsevier B.V. All rights reserved. SciVerse® is a registered trademark of Elsevier Properties S.A., used under license. ScienceDirect® is a registered trademark of Elsevier B.V.

3 of 3 03/21/2011 5:14 PM