BIOLOGY PRACTICAL

Experiment on Thermoregulation
 2° C

11°C

20 cm3 2°C
For F1,
0.25
 BIOLOGY Practical
Experiment:
Production of Carbon dioxide during anaerobic respiration
Anaerobic Respiration
Anaerobic means without air. Sometimes there is not
enough oxygen around for animals and plants to respire,
but they still need energy to survive, so they carry out
respiration in the absence of oxygen to produce the
energy they require. As the respiration takes place in the
absence of oxygen, incomplete oxidation of food occurs
and much less energy is released. However, carbon
dioxide is still produced. This is called anaerobic
respiration and the process occurs in the cytoplasm.
The word equation for anaerobic respiration in
plants/Yeast is:
The chemical equation is:

Objective:
To show experimentally that carbon dioxide is released during
respiration.

Apparatus and Materials Required:

A beaker, yeast powder, a cork/bung with a hole, a glass tube/
delivery tube bent at right angles at two places, a small test tube.

Procedure:
 Result:
The numbers of Air bubbles indicate that CO2 is produced during
anaerobic respiration. Actually, the yeast will respire and produce
CO2, which will form bubbles. The air present in the bent glass tube
moves into the beaker containing water. The bubbles in the water
will be further counted.

Precautions:
Keep the test tube airtight.

Factors affecting Respiration:

Here are some of the few factors that affect the rate of respiration.

 Temperature- At a very high temperature, the rate of respiration decreases with time and
at very low temperature, the respiration rate is insignificant. Optimum temperature for
respiration is 20 - 30oC.
 Carbon dioxide concentration - Increase in CO2 concentration and absence of
O2 adversely affects the rate of aerobic respiration.
 Light- Light controls respiration by raising the temperature of an organism.
 Water- The respiratory rate increases with the increase in water content of the respiring
organism.

Effect of Glucose and Sucrose on the activity of yeast:

Yeast breaks down Sucrose into glucose and fructose with the help of enzyme invertase. More
amount of Carbon dioxide will be produced as there will be two units of monosaccharides( one is
glucose, another one is fructose). Glucose and Fructose will be further broken down by Yeast with
the help of enzyme zymase and will produce lot of bubbles. There are lots of unicellular organisms
which secrete enzymes to break down sucrose.”
Learning Outcomes:

1. Students understand the terms respiration, aerobic and anaerobic respiration and ATP.
2. Students understand the two types of respiration.
3. Students understand the factors affecting the rate of respiration.
4. Students do the experiment better in the real lab.

 Presence of Carbon dioxide by using Lime water:

Respiration in humans can be studied with lime water. Carbon dioxide reacts with lime water on
respiration to form calcium carbonate (CaCO3) that turns lime water milky.
Ch. Heredity