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In Plant Tissue Culture
In Plant Tissue Culture
Kamala pujhari
Kanwal singh Chauhan
Who started green revolution?
Norman Borlaug
William S Gaud
Henry A Wallace
Pual C Mangelsdorf
The process of expression of foreign genes in a plant is called___________
Genetic transformation
Transgenesis
Gene expression
Cell hybridization
Name the first transgenic virus resistant plant?
Tobacco
Rice
Cotton
Tomato
Which of the following is supplemented with vitamin A in order to improve
its nutritional quality?
Rice
Cotton
Tomato
Potato
The controversy regarding the use of Bt corn is that it
Is potentially harmful to monarch butterflies
Is a potential allergen to humans
Both (a) and (b)
Can contaminate groundwater
Which of the agricultural challenges below cannot be solved with transgenic
techniques?
Public preference for organic vegetables
Public concern about safety of synthetic pesticides
Crops are killed by a virus
Crops are damaged by frost
The first transgenic plants expressing engineered foreign genes were tobacco
plants produced by the use of
Agrobacterium tumefaciens
Bacillus thuringiensis
Arabidopsis thaliana
Streptomyces hygroscopicus
Transgenic plants
Contain foreign genes in their cells
Can’t be used to produce human antibodies
Both (a) and (b)
Are plants that differ in geographical locations
For developing herbicide resistance in transgenic plants which of the
following approach/(es) is/are used?
Target molecule is made insensitive to herbicide
Target protein is overproduced
A pathway should be introduced that detoxify the herbicide
All of the above
Which of the following is NOT a characteristic of a transgenic crop?
Herbicide resistance
Bt insect resistance toxin
Increased methionine content
None of these
The process of adding unwanted substances to the food, with similar
appearance/colour for making profits is called
Adulteration
Doping
Lyophilisation
Cryopreservation
This is an example of biological hazard
Salmonella
Cleaners
Antibiotics
Dirt
Under the Prevention of food Adulteration Act (PFA Act), when is the food
said to be adulterated
If any ingredient is injurious to health
If it is obtained from a diseased animal
If spices are sold without their essence
All of the above
This is an adulterant
Pesticide
Yellow soil
Pepperoil
All of the above
Which one is INCORRECT among the following?
Food adulteration increases the quality of food
Food adulteration is done as part of business strategy
Food adulteration is done to increase quantity of food production and
sales.
Food adulteration lacks effective food laws
The adulterants are added as a deliberate act with aim to increase profit. E.G.
sand, marble chips, stones, chalk powder, etc. is called
Intentional adulteration
Incidental adulteration
Metallic adulteration
Misbranding
Adulterants are found in food due to negligence, ignorance or lack of proper
facilities. E.G. Packaging hazards like larvae of insects, droppings, pesticide
residues, etc.
Incidental adulteration
Intentional adulteration
Metallic adulteration
Concealing quality
Which one is INCORRECT among the following?
There should not be strict actions regarding the punishment for those
who are involved in food adulteration.
There must be proper surveillance of the implementation food laws.
There should be periodical training programmes for Senior
Officer/Inspector/Analysts for food safety
There should be consumer awareness programmes organized by
holding exhibitions/seminars/training programmes and publishing
pamphlets.
The desire to maintain a safe laboratory environment for all begins with
_________?
Prevention
Microbiology
Ubiquity
Accidents
Which of the following type(s) of Personal Protective Equipment (PPE) is
frequently used?
Gloves
Safety glasses
Lab coats
All of the above
What is the name of the procedure performed under sterile conditions to
eliminate contamination in hopes to obtain a pure culture of one type of
microorganism?
Aseptic condition
Sterilization condition
Pathogen technique
Disinfectant technique
What is needed as a source of nutrient for the growth and reproduction of
microbes?
Media
Reagent
Pathogens
Bacteria
Pruning is an essential cultural activity in fruit plants because
To provide proper shape
To provide strong structure
To beautify the plant
All of the above
Growing of flowers and ornamental plants is called
Olericulture
Floriculture
Both a and b
Pomology
Growing of fruits in garden and farm is called
Pomology
Olericulture
Floriculture
Vegetable science
Which type of garden are of geometric design and symmetrical shape?
Formal gardens
Informal garden
Free style garden
Natural garden
The style reflects the naturalistic effect of total view and represents natural
beauty is called
Informal garden
Formal garden
Free style garden
Natural garden
The combination of both formal and informal garden is called
Free style garden
Formal garden
Informal garden
Natural garden
An enclosed work area that has sterile air moving across it. The air moves
with uniform velocity along parallel flow lines.
Horizontal laminar air flow
Autoclave
Distillation unit
Oven
The practice of working with cultures in an environment free from
microorganisms.
Sterile technique
Septic technique
Pathogen technique
Sub culturing
Which among the following is INCORRECT regarding plant tissue culture?
Cell selection decreases the potential number of individual in a
screening program
To quickly produce mature plants
To mass propagate plants for commercial use
To produce disease free plants
Which among the following is INCORRECT regarding laboratory
requirement for plant tissue culture?
Plant tissue culture can be carried out in an open area conditions.
Localize each portion of the tissue culture procedure in specified place
in the laboratory
Use glassware that has only been used for plant tissue culture and not
for other experiments.
Use only high purity water in tissue culture procedure
Arrange the following steps of micropropagation in correct order
Shoot production b) Transfer to natural environment c) Pre
transplant d) Sterilization
d, a, c, b
a, d, c, b
d, a, b, c
a, c, d, b
Culture medium consists of all except for
Ethyl alcohol
Microelements
Plant growth hormones
Vitamins
Which one is not the sterilization method?
Inoculation
Autoclaving
UV radiation
Dry heat
A filter capable of screening out particles larger than 0.3 µm and are used in
laminar air flow cabinets (hoods) for sterile transfer work are called
High efficiency particulate air filter
High pass filters
Notch filters
Low pass filters
What is/are the advantage of micropropagation?
It helps in rapid multiplication of plants.
A large number of plantlets are obtained within a short period and
from a small space.
Both a and b
The rare plant and endangered species cannot be multiplied by this
method
Which media is not used in plant tissue culture?
Eagle media
MS media
White’s media
B5 media
Which one is not the surface sterilization agent?
Calcium peroxide
Calcium hypochlorite
10- 12% hydrogen peroxide
Alcohol
Gaseous plant hormones
Ethylene
Auxin
ABA
Cytokinin
Plant stress hormones
ABA
Auxin
Cytokinin
Gibberellins
General yellowing of the plant tissue due to lack of chlorophyll.
Chlorosis
Necrosis
Mottling
Burning
Death of plant tissue; tissue browns and dies
Necrosis
Chlorosis
Burning
Stunting
Decreased growth; shorter height of the affected plants.
Stunting
Necrosis
Chlorosis
Burning
Which among the following is micronutrient?
Boron
Nitrogen
Potassium
Phosphorous
Which among the following is macronutrient?
Magnesium
Copper
Iron
Zinc
During the germination of seeds, the seed coat ruptures due to
Massive imbibition of water
Differentiation of cotyledons
Sudden increase in cell division
Massive glycolysis in cotyledons and endosperm
Seed dormancy allows the plants to
Overcome unfavourable climatic conditions
Develop healthy seeds
Reduce viability
Prevent deterioration of seeds
Which of these compounds can induce seed dormancy
ABA
Gibberellins
Ethylene
Potassium nitrate
Seed dormancy is
Failure of a seed to germinate under unfavourable condition
Failure of a seed to germinate under favourable condition
The ability of a seed to germinate under unfavourable condition
The ability of a seed to germinate under favourable condition
The process of weakening the seed coat to break dormancy is called
Scarification
Photoperiodism
Vernalization
Stratification
Photoblastic seeds are seed
Affected by light for germination
Not affected by light for germination
Independent of light for germination
Affected by light and temperature for germination
Abscisic acid is also called as
Abscisin I
Abscisin II
Dormin
All of the above
During water stress rise in ABA levels initially cause
Stomatal closure
Stomatal opening
Reduced transpiration
Increased transpiration
ABA is an
Antigibberelin
Anticytokinin
Ethylene inhibitor
Antiauxin
Which of the following hormone functions as an anti-transpirant
ABA
Auxin
Gibberellins
Cytokinin
Which of the following is not the function of auxin?
Inducing dormancy
Enhancing cell division
Inducing callus formation
Maintaining apical dominance
Which of the following is the natural cytokinin?
Zeatin
Isopentyl adenine
6-Amino purine
6- Isopentyl adenine
Which of the following is not the function of cytokinin?
Induce dormancy
Delay senescence
Promote cell division
Counteract apical dominance
Gibberellins was named after a fungus called
Gibberella fujikurio
Gibberella acuminata
Gibberella gaditijirrii
Gibberella africana
All are the functions of gibberellins except
Promote dominance
Promote cell elongation
Promote elongation of internode
Promotes parthenocarpic fruit formation
Genetic dwarfism can be nullified by spraying
Gibberellin
Auxin
Zeatin
Ethylene
External conditions that adversely affect the growth, development or
productivity of plant is called
Plant stress
Pleiotropy
Geotropism
Vernalization
Plant stresses triggers a wide range of plant responses except for
Promotes growth of the plant
Gene expression
Cellular metabolism
Affects crop yields adversely
Which one is INCORRECT regarding Low Temperature Stress
Low temperatures increases potential agricultural productivity by
limiting the crops or varieties that can be grown in a particular area
Low temperatures can damage plants both by a chilling effect and
freezing
Chilling effect, leads to physiological and developmental
abnormalities
Freezing, leads to cellular damage directly or via cellular dehydration.
To overcome the stress the plants use the following ways except for one
which leads to death of plant
Susceptible
Resistance
Avoidance
Tolerance
The crosses are made between the plants belonging to two different varieties.
Inter-varietal hybridization
Intra-varietal hybridization
Inter-specific hybridization
Intra-specific hybridization
The crosses are made between two different species of the same genus.
Inter-specific hybridization
Intra-specific hybridization
Inter-varietal hybridization
Intra-varietal hybridization
Essential elements which are present in easily detectable quantities, 1-10 mg
per gram of dry matter
Macronutrients
Micronutrients
Trace elements
Non-essential elements
Essential elements which are required by plants in small amounts, equal or
less than 0.1 mg/gm. of dry matter
Micronutrients
Macronutrients
Trace elements
Non-essential elements