FIRST PROFESSIONAL MBBS FOURTH SEMESTER

EXAMINATION SESSION-2007-2008
SUBJECT - BIOCHEMISTRY

1)- CODONS ARE PRESENT IN:

(A) Template strand of DNA.

(B) mRNA.
(C) tRNA.
(D) rRNA.
(E) Nucleotides.

2)- EXTRA NUCLEAR DNA:

(A) Dosen’t encode proteins.

(B) Encodes proteins which are used in ribosomes.
(C) Encodes proteins which are used in mitochondria.
(D) Encodes proteins which can be used anywhere in the cell.

3)- HYPOXANHINE MAY BE PRESENT IN:

(A) DNA.
(B) mRNA.
(C) tRNA.
(D) rRNA.
(E) All nucleotides.

4)- WHICH OF THE FOLLOWING STATEMENTS REGARDING A DOUBLE-HELICAL

MOLECULE OF DNA IS TRUE:

(A) All hydroxyl groups of pentoses are involved in linkages.

(B) Bases are perpendicular to the axis.
(C) Each strand is identical.
(D) Each strand has parallel, 5` to 3` direction .
(E) Each strand replicates itself.

5)- POLYMERASE CHAIN REACTION (PCR) INVOLVES THREE BASIC STEPS IN

WHICH OF THE FOLLOWING ORDERS?

(A) Annealing denaturation extension.

(B) Annealing extension denaturatoin.
(C) Denaturation annealing annealing.
(D) Extension annealing denaturation.
(E) Extension denaturation annealing.

6)- IN CONTRAST TO DNA POLYMERASE, RNA POLYMERASE:

(A) Adds nucleoside monophosphates to the frowing polynucleotides.

(B) Edits as it synthesizes.
(C) Fills in the gap between okazaki franments.
(D) Synthesizes RNA primer to initiate DNA synthesis.
(E)Works only in a 5` to 3` direction.

7)- A PROMATER SITE ON DNA:

(A) Codes for RNA polymerase.

(B) Initiates transcription
(C) Regulates termination.
(D) Transcribes repressor.
(E) Translates specific proteins.
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8)- THE STATEMENTS CHARACTERISE DNA REPLICATION :

A) RNA and DNA chains are linked covalently.

B) Growth of the new DNA chains occurs in the 5` to 3` direction.
C) Growth of DNA chain is discontinuous.
D) Overall growth of DNA chain is bidirectional.
E) DNA is synthesized in a 3` to 5` direction on one parental strand and in a 5` to 3`
direction on the other.

9)- SICKLE CELL ANAEMIA IS THE CLINICAL MANIFESTATION OF

HOMOZYGOUS GENES FOR AN ABNORMAL Hb MOLECULE. THE
MUTATIONAL EVENT RESPONSIBLE FOR MUTATION IN THE
B-CHAIN IS :

A) Deletion.
B) Insertion.
C) Point mutation.
D) Crossing over.
E) Non-dysjunction.

10)- TRANSLOCASE IS AN ENZYME REQUIRED IN THE PROCESS OF :

A) DNA replication.
B) RNA synthesis.
C) Initiation of protein synthesis.
D) Elongation of peptides.
E) Termination of protein synthesis.

11)- NONSENSE CODONS BRING ABOUT :

A) Amino acid activation .

B) Initiation of protein synthesis.
C) Termination of protein synthesis.
D) Elongation of polypeptide chains.
E) Post translational modification of proteins.

12)- IN THE PROCESS OF TRANSCRIPTION, THE FLOW OF GENETIC

INFORMATION IS FROM :

A) DNA to DNA.
B) DNA to protein.
C) RNA to protein.
D) DNA to RNA.
E) Protein to RNA.

13)- THE ANTICODON REGION IS AN IMPORTANT PART OF THE

STRUCTURE OF :

A) r-RNA.
B) t-RNA.
C) m-RNA.
D) hn-RNA.
E) DNA.

14)- WHICH OF THE FOLLOWING IS TRANSCRIBED DURING

REPRESSION :

A) Structural gene.
B) promoter gene.
C) Regulator gene.
D) Operator gene.
E) Non structural gene.
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15)- DNA POLYMERASE PRESENT IN MITOCHONDRIA IS:

(A) Gamma.
(B) Alpha.
(C) Delta.
(D) Beta.
(E) Epsilon.

16)- AZASERINE, THE ANTICANCER DRUG INHIBIT:

(A) Formation of PRPP.

(B) Utilization of formyl THFA.
(C) Formation of glycinamide ribonucleotide.
(D) Phosphoribosyl amido transferase.
(E) Multienzyme complex.

17)- REGARDING DE NOVO SYNTHESIS OF PURINES:

(A) Extra hepatic tissues are the main site of formation.

(B) Are formed as adenine and guanine.
(C) Carbondioxide (CO2) fixation reaction require biotin / ATP.
(D) Aspartic acid and glycine are completely utilized.
(E) Inosine monophosphate is concerted to AMP and GMP.

18)- SALVAGE PATHWAY:

(A) Convert nucleosides to nucleotides .

(B) Convert the free purines to nucleotides.
(C) Source of ribose is HMP-shunt.
(D) Mainly are salved by Nucleoside phosphorylase + Guamosine kinase.
(E) Converts free pyrimidine to nucleosides.

19)- SECONDARY GOUT RESULTS FROM:

(A) Abnormal PRPP synthetase.

(B) Deficiency of G-6-P phosphatase.
(C) Deficiency of enzymes of salvage pathway.
(D) Glutathione reductase variant.
(E) Increased tissue breakdown in starvation .

20)- REGARDING URIC ACID:

(A) Is end product of pyrimidene bases.

(B) Raised conc: is found in vegetarians.
(C) Potassium urate crystals form tophi.
(D) Deficiency of xanthene oxidase causes Hypourecemia.
(E) Normally conc: is high in females as compared to males.

21)- CAUSE OF LEASH NYHAN SYNDROME IS:

(A) Purine biosynthesis.

(B) Pyrimidine salvage.
(C) Neuclotide breakdown.
(D) Purine salvage.
(E) Pyramidine biosynthesis.
22)- AMMONIA IS TRANSPORTED FROM MUSCLE TO LIVER IN FORM OF:

(A) Glutamine.
(B) Alanine.
(C) Aspartic acid.
(D) Asparagine.
(E) Glutamnic acid.
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23)- WHICH AMINO ACIDS, ACTIVELY PARTICIPATE IN TRANSAMINATION:

(A) Aspargine and glutamine.

(B) Alanine and asparate.
(C) Alanine and Glutamic acid.
(D) Arginie and protein .
(E) Lencine and lysine.

24)- FORMATION OF SEROTONIN:

(A) Tryptophan is carboxylated.

(B) It essentially requires tetralydrobiopterin.
(C) Tetrelydrobiopterin is regeuereted by FAD.
(D) It is regenerated by NAD.
(E) Reserpine inhibit its release.

25)- MOST PREVALENT INBORN ERROR OF PROTEIN METABOLISM IS:

(A) Albinism.
(B) Phenyl ketonuria.
(C) Alkaptonuria.
(D) Maple syrupurine disease.
(E) Hyper ammonemia type I.

26)- UREA CYCLE:

(A) First line of defense against ammonia toxicity.

(B) The major regulatory step is catelysed by CPS-II (Carbamoyl phosphate synthase I)
(C) The enzyme are located in one compartment
(D) Energy is not required for synthesis
(E) During starvation the activity of urea cycle enzymes is elevated.

27)- TNTRANS METLYLATION REACTION:

Methyl Aceeptor Methylated Product

(A) Guanido Acitic Acid --------------------------- Creatinine

(B) Norepinephrine ---------------------------Metanephirne
(C) Ethandamine --------------------------- Carnitine
(D) Histadine --------------------------- Histamine
(E) Acetylserotonin ---------------------------Melatonin

28)- REGARDING GLUTATHIONE:

(A) It is glutamyl seryl glycine.

(B) For synthesis 3 moles of ATP are required.
(C) Help in activation of Insulin.
(D) Glutathion is necessary for oxidation of met-hemoglobin.
(E) Necessary for Fe (Iron) absorption as it converts Fe+++ --------Fe++.

29)- REGARDING LIVER FUNCTION:

(A) It maintains acid base balance.

(B) It is the site of Hb synthesis in adult man.
(C) It supplies glucose to body during well fed state.
(D) Total body cholesterol is synthesized in it.
(E) It is the site for Denove purine synthesis.

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30)- THE FATTY ACID SYNTHASE COMPLEX OF HUMAN CONTAIN:

(A) Covalently bound acyl carrier protein.

(B) Four different kinds of sub units each having several enzyme activities .
(C) Seven pairs of identical sub unit.
(D) Covalently bound pyridoxal phosphate.
(E) two identical sub unit each having several enzyme activities .

31)- REGARDING CHOLESTEROL SYNTHESIS:

(A) Only small fraction is synthesis in the body.

(B) Dose not require activation of HMG CoA reductase.
(C) It is regulated by para thyroid hormone.
(D) It takes place in mitochondria .
(E) Some fraction is synthesized in skin.

32)- KETONE BODIES ARE INCREASED IN BLOOD:

(A) By excessive lipolysis.

(B) In controlled diabetic mellitus .
(C) Over feeding of carbohydrate diets.
(D) Following of deep breathing.
(E) In phenyl ketonuria.

33)- COMPLETE OXIDATION OF PALMITIC ACID PRODUCES:

(A) 6 acetyl CoA.

(B) 8 FADH2.
(C) 36 ATPs.
(D) 3 NADH.
(E) 129 ATP.

34)- FOLLOWING FACTORS CAUSE HYPOCHOLESTEROLEMIA:

(A) Malabsorption syndrome and thyroid stimulating hormone.

(B) Smoking.
(C) Obstructive jaundice.
(D) Diabetes mellitus.
(E) gluco carticoids.

35)- CHOLINE RESIDUE IS PRESENT IN WHICH OF THE FOLLOWING LIPIDS:

(A) Glucocerebrosides.
(B) Gangliosides.
(C) Cholesterol.
(D) Sphingomylin.
(E) Phosphatidic acid.

Signature ___________
 SHORT ESSAY QUESTIONS

Q.1. When ketogenesis takes place? Write the steps of Synthesis.

Q.2. Describe urea cycle Discuss causes of Uremia.

Q.3. Write difference between RNA and DNA

Q.4. Describe the Metabolic role of liver Mention liver function tests (LFT)

Q.5. Describe any two inborn errors of protein metabolism

(a) Phenyl ketonuria

(b) Alkaptonuria

(c) Maple syrup urine disease

Q.6. Describe replication

Q.7. Describe the steps of Beta – oxidation and energy obtained from
palmatic acid.

Q.8. Describe the salvage pathway of purines.

Signature ___________
 KEY SHEET FOR BCQS

FIRST PROFESSIONAL MBBS FOURTH SEMESTER

EXAMINATION SESSION-2007-2008
SUBJECT - BIOCHEMISTRY

QUESTION NO. KEY

1 B
2 C
3 C
4 B
5 C
6 D
7 B
8 E
9 C
10 D
11 C
12 D
13 B
14 C
15 A
16 D
17 E
18 B
19 E
20 D
21 D
22 B
23 C
24 B
25 A
26 E
27 E
28 E
29 E
30 A
31 E
32 A
33 E
34 A
35 D
Name of Paper Setter Prof. Zafar Ali Pirzado Signature ___________

OFFICE OF THE HEAD OF BIOCHEMISTRY DEPARTMENT CMC LARKANA.

No.CMC/Biochem:/ Dated__________2009.

To

The Controller of Examinations,

Liaquat University of Medical & Health Sciences,
Jamshoro.

Subject: QUESTION PAPER FOR FIRST PROFESSIONAL MBBS, FOURTH SEMESTER

EXAMINATION SESSION 2008-2009 IN THE SUBJECT OF BIOCHEMISTRY

Reference: Your office letter No. LUMHS/Exam/Ts/1199, dated 29-10-2009.

With reference your office letter referred to above, I am sending herewith the

Question Paper for First Professional MBBS Fourth Semester Examination Session

2007-2008 in the subject of Biochemistry along with Master key.

(DR. ZAFAR ALI PIRZADO)
PROFESSOR AND HEAD OF
BIOCHEMISTRY DEPARTMENT
C.M.C. / (SMBBU), LARKANA.