Professional Documents
Culture Documents
D I Nutrition Updated
D I Nutrition Updated
1
DIPLOMA IN NUTRITION
FUNDAMENTALS OF NUTRITION SCIENCE
AKASH SEHRAWAT, H
HP, CPT
2
www.fabulousbody.com
3
Table of Contents
Disclaimer…………………………………………………………………………...……………..7
Preface………………………………………………………………………………………….…….8
Module 2: Macronutrients……………………………………..………………..28
Lecture 1: Carbohydrates…..……………………...……………………………………...28
Lecture 3: Proteins..…………………………………………………………………………...37
Lecture 5: Fats……..…………………..…………………………………………………………46
Module 3: Micronutrients………..……………………………………………...57
Lecture 1: Fat-soluble Vitamins: A, D, E & K……...…………………………….….58
4
Lecture 2: Probiotics…....…………………………………….…………………………...109
Lecture 3: Water…………....…………………………………….………………………….113
Lecture 4: Your Body Cannot Absorb More Than 30gm Of Protein In
One Sitting………………………………………………………………………………………..133
Lecture 2: Refined Carbohydrates & Added Sugars Are Bad For You..148
5
References………………………………………………………………………………………..156
6
Disclaimer
The information in this book has been carefully researched, and all
efforts have been made to ensure accuracy. The publisher and
author assume no responsibility for any injuries suffered or
damages or losses incurred during or as a result of following the
exercise and diet program in this book.
7
Preface
8
sources, and their functions and requirements. The last lecture of
this module will help you understand how to determine macro
ratios of any diet.
Along with visually pleasing lecture slides, you will find a voiceover
presentation which explains all the slides in detail plus you will find
the accompanying script (this master manual) in case you want to
read (helpful for people who are hearing impaired)
Once you have gone through the course material and are
confident enough please take the final exam and score above 50%
to secure yourself a CPD Certificate.
9
-Health & Fitness Professionals who want to help their clients with
general nutrition tips/guidelines.
-Understand what the Macronutrients are and the role they play in
our bodies.
10
11
Wikipedia:
“Nutrition is the study of nutrients in food, how the body uses them,
and the relationship between diet, health, and disease.”
12
Carbohydrates
Proteins
Fats
Vitamins and minerals are the micronutrients that are required in
small quantities.
13
When the energy intake is more than energy expenditure, you gain
weight. The body stores the extra calories as fat.
When the energy intake is less than the energy expenditure, you
lose weight.
In the resource manual, please do find the link for an article titled:
Calories In, Calories Out: CICO: Does it Matter?
14
Since we’ll be using the term calorie so many times, it does not
make sense to use kilocalorie every time, but it's important to
know the difference.
Our brains, our muscles — every cell in our body — require energy
to function in its optimal state.
15
Junk/Fast foods have calories in them as they are also made up of
carbs, proteins, and fats, but those calories are termed as ‘empty’
calories.
The term ‘Empty’ means these calories have very little or no
nutrients in them.
For example, pizza contains some nutrients like cheese which has
calcium and protein but other foods like candies, soft drinks
contain only empty calories and no nutrients.
Let’s define energy expenditure i.e. calories out in the next lecture.
16
Let’s start with the first component of energy expenditure that is
BMR.
Just imagine lying down on a sofa the whole day doing nothing at
all!
17
Another name for BMR is RMR, which stands for resting metabolic
rate.
There are various formulas for estimating your BMR. Let’s look at
the two most popular ones.
18
So, let’s say you are 170 pounds. If you know your weight in
kilograms, then simply multiply it by 2.2 to get your weight in
pounds.
Your BMR number may vary depending on the equation you use,
but let me tell you, you cannot calculate an exact number, so don’t
even try. Estimates are fine.
And they use the simple equation: Your body weight in pounds *
10.
There is another easy way out and that is to use the TDEE
calculator on my website www.fabulousbody.com
19
When you eat protein, 20 – 30% of the protein calories are burned
digesting it. This means a high protein diet will have a higher TEF.
The most common estimate for the total thermic effect of food is
around 10% of your BMR.
20
Let’s say your BMR is 1700 calories. Quite simply, your TEF would
be 1700*10= 170 calories.
Now, we are left with TEA, which stands for the thermic effect of
activity. Let’s continue in the next lecture.
21
TEA is the calories you burn through activity, i.e. your training and
workouts.
22
BMR is the number of calories you expend when you are lying
down on the sofa the whole day.
Let’s say you start to move around your house, you go to your
office and perform other daily chores.
Because of this basic movement, there will be extra calorie burn in
addition to your BMR.
For this sedentary lifestyle, the thermic effect of activity can be
calculated by multiplying BMR by 0.2.
You can put your clients in the category of lightly active if they do
some light movement 2 – 3 times a week.
23
Or
Or
Or
24
This means that beyond these workouts, their step count is limited
to ~5000 steps a day.
Their step count is easily over 10,000 steps and may go up to
15,000 steps per day at least five times a week.
Or
25
This means they often work out twice a day a few times a week.
Or
Most athletes also have a very strict schedule that includes a few
strength training sessions combined with cardiovascular training.
26
TDEE Calculation
27
M2: Macronutrients
L 1: Carbohydrates
28
In Lecture 6, I’ll provide you with Sally’s example that will help you
determine the macronutrient profile of a diet.
Well, I'll try to answer this question for you towards the end of this
lecture when we will discuss the glycemic index, but first, let's
begin with the definition of carbohydrates, the function that
carbohydrates play in our bodies, it’s classification and main food
sources.
Functions of carbohydrates
29
As you will learn in the next lecture about protein that proteins are
the main building blocks of a human body and when you eat
enough carbs, then instead of protein, carbs are used for energy,
sparing the protein to do its essential job that is to build muscle
tissues and manufacture hormones.
Carbohydrate Classification
30
When you eat a lot of refined carbs, like white rice, pasta, bagels,
and sugar-sweetened beverages over time, the cells in your body
start to resist insulin because so much is released every time you
eat those foods.
31
Does this mean that the simple sugars in fruits are also bad?
But the sugars in junk and fast foods, like candies, biscuits, cakes,
and sugar-sweetened beverages (SSBs) break down very quickly,
as there is little or no fiber in them.
Most junk and fast foods that are high in simple carbohydrates are
also devoid of vitamins, minerals, and other nutrients, resulting in
mostly empty calories.
32
For now, let's move on to the glycemic index. This is one of the
most important concepts in nutrition so please listen carefully.
33
M2: Macronutrients
Foods with a low GI value (55 or less) are more slowly digested and
absorbed, causing a slow rise in blood glucose. Less insulin is
needed to combat this gradual rise in blood sugar.
34
Examples of foods with a high GI value are white bread, white rice,
most breakfast cereals, candies, and cakes.
35
The same effect happens when you eat foods with a low GI value.
Sugar is digested and absorbed in the body slowly, leading to a
gradual increase in blood sugar, which the body can use better.
When you eat foods with high GI values, your body digests and
absorbs them quickly, causing a sudden rise in blood sugar levels,
and your pancreas must pump large quantities of insulin to
combat it.
Insulin is a hormone that helps get the blood sugar (glucose) from
the blood to the cells.
Over time, when you eat more refined carbs and your body pumps
out a lot of insulin, the cells in your body start to resist it.
So, as a thumb rule eat carbohydrates that are low on the GI scale.
So this was all about carbs and in the next two lectures we shall
discuss proteins.
36
M2: Macronutrients
L 3: Proteins
blocks.
37
Proteins are made up of amino acids, which are like the building
blocks of the human body.
When you eat proteins, your body breaks it down into its
constituent parts—amino acids.
Our bodies can’t make some of these amino acids; hence they are
known as essential amino acids. These nine essential amino acids
we need to get from our diets.
Please note that Histidine is the only essential amino acid that is
essential for infants!
Protein Classification
In terms of a diet, protein sources are categorized according to
how many of the essential amino acids they provide.
38
39
This is to ensure that your body is getting all the essential amino
acids as most plant-based proteins are deficient in one or two
essential amino acids.
A huge and popular myth which you may have come across is this:
40
M2: Macronutrients
But please note, this intake is fine for people who live a sedentary
lifestyle and represents an intake level necessary to replace losses
and avert deficiency.
41
42
43
So if you are a 70kg individual, you don’t need more than 112g
(70*1.6) of daily protein for maximal muscle gains.
RDA guidelines for protein intake are less than the amount
required for active individuals and should thus be adjusted
according to one’s type of activity, as explained above.
44
Aim to get your protein from various food sources to get all the
essential amino acids, especially if you are a vegetarian/vegan.
Make sure to check the Resource Manual for the article links
relevant to this lecture.
45
M2: Macronutrients
L 5: Fats
What is fat?
46
Functions of Fat
Fats are the largest and the cleanest source of energy and they
provide 9 calories per gram more than double that of proteins and
carbohydrates.
All fats and oils, regardless of whether they are of vegetable or
animal origin, are some combination of saturated fatty acids,
monounsaturated fatty acids, and polyunsaturated fatty acids.
Good Fats
47
Bad fats are mainly found in margarine, pastries, corn, and soy oil,
basically, all processed vegetable oils, and trans fats are the worst
kind of fat.
Food for thought: Please see this amazing fact that all the good
fats are fats that exist in nature as it is, where all bad fats are
man-made. If they are processed, like coconut oil or ghee, its best
to choose the cold-pressed ones as the processing is done in such
a manner that helps preserve maximum nutrients.
Saturated fats
Saturated fats have gotten a lot of attention in the press over the
years, but these fats have been unfairly demonized.
Do not fear whole milk and whole eggs. Embrace them, and eat
them as nature intended.
Do not fear saturated fats. They are your best friends and should
be responsible for the majority of the calories you eat from fat.
Avoid trans fats at all costs; they are the worst kinds of fat and
wreak havoc on your body.
48
However, I have penned a few articles that will help you include
the right type of fats in your diet.
Lastly, please note that I am simply an educator and the best I can
do is to provide you with anecdotal evidence and hard science to
prove my point.
Everyone has the right to their opinion the same way everyone has
the right to reject something that they don't agree with.
Do your own research, read the posts that I have linked up. Ask me
questions if you have any questions or doubts.
49
In the next and final lecture, I will provide you a strategy that can
act as a starting point for any laymen to figure out their optimal
macronutrient ratio in their diets.
Although this is not a diet planning course, and you’ll find a lot of
information about formulating your diet in that diploma but in the
next lecture, I will give you a good starting point.
50
M2: Macronutrients
Let's say you have a client, and her name is Sally. She is 35 years of
age and weighs 140 lbs.
Sally’s protein intake comes out to be: 1.8* 63.6kg (140)= 115g
(rounded off)
51
However, for most diets, a healthy fat range lies between 25 to
40%.
Again this range will vary on your client’s food choices which are
intrinsically linked with their ethnic background.
The details of the macro splits for different types of diets and
cuisines around the world is a vast topic which I have covered in
my Diet Planning Diploma.
As per Sally’s dietary preference, her average fat intake comes out
to be 30% of her total calorie intake.
1g of fat is 9 calories.
52
The healthy fat calories can come from nuts, seeds, egg yolk,
whole dairy like milk, yogurt, and coconut oil/ghee.
To summarize so far:
The remaining calories will come from Carbohydrates which will be
40% of the total calorie intake.
The calories will mainly come from complex carbs like mashed
potatoes, sweet potatoes, brown rice, steel-cut oats, dairy and
whole-grain bread, etc.
53
A low carb diet will range from 4 to 20% of 2000 calories which will
come to about 20-100 grams of carbs eaten per day.
Less than 100g of carbs is actually very less, considering there are
carbs in literally every processed food item often disguised under
various names.
A Standard Ketogenic diet has no more than 20g of carbs or less in
it.
54
All of you trying to lose weight and improving your health will do
well on moderate carbs with some of you may do very well on a
low carb diet like a kept diet.
Needless to say that a Keto warrior will eat at least 70% of his
calories from fat.
If you can learn and imbibe just this one thing from this course
and that is that healthy fats are indeed very good for you, I will
consider the time spent on this course a victory.
And last but not the least, proteins are always calculated by taking
into account one’s body weight.
The least one should go for is 1.2g/kg of BW and ideally one should
stay in the range of 1.2 to 2g/kg of bodyweight.
55
Ok, then, I guess I didn’t overwhelm you with this and I hope you
found this lecture informative. Now let's move on micronutrients.
56
M3: Micronutrients
Diploma in Nutrition. I hope you are not only enjoying this course
but also finding it useful!
Vitamins
Vitamins are organic compounds that are needed in small
quantities to sustain life. Most vitamin needs come from food as
the body cannot manufacture them.
There are 13 known vitamins, out of which four are fat-soluble,
and nine are water-soluble.
Fat-Soluble Vitamins are A, E, D & K.
Water-soluble are Vitamin B1, B2, B3, B5, B6, B7, B9, B12, and
Vitamin C.
Let's start by discussing the Fat-Soluble Vitamins in the next
lecture.
57
M3: Micronutrients
and the liver. They can stay in the body for days and sometimes
even months.
Let's start with Vitamin A.
Vitamin A
Vitamin A is a group of organic compounds that the human body
needs in minimal amounts. The body cannot manufacture it, and
therefore it has to be included in our diet.
Two forms of vitamin A available in the human diet are preformed
vitamin A found in animal foods and provitamin A found in plant
foods.
The most important provitamin A is beta-carotene. 20
Vitamin A Functions
Vitamin A plays many roles in our bodies. It regulates the immune
system, plays a role in bone & tooth development and
reproduction,
58
It is also vital for hair growth and essential for maintaining the
light-sensing cells in the eye.
Vitamin A Food Sources
Preformed vitamin A is abundantly available in animal products
like milk and liver and some fish.
Plant-based foods like carrots, sweet potatoes, and green leafy
vegetables have provitamin A.
Vitamin A Deficiency
Vitamin A deficiency is a leading cause of preventable blindness in
children worldwide. About a quarter to half a million children
become blind every year due to vitamin A deficiency.
The most common symptom of vitamin A deficiency is
xerophthalmia. 21
Children in Africa and South Asia are the most affected and what is
unfortunate is that half of the children who become blind die
within a year of losing their sight. 22,
23, 24
Vitamin A deficiency is rare in most of the developed nations.
Vitamin D
Vitamin D, also known as calciferol, is a fat-soluble vitamin, which
means it can be stored in the body for a long time, just like
vitamins A, E, and K.
59
60
61
62
Vitamin E deficiency
Vitamin E deficiency is uncommon and is never detected in
otherwise healthy people.
It happens most often in diseases that impair the absorption of fat
or vitamin E from food, such as cystic fibrosis and liver disease.
Vitamin E deficiency symptoms include muscle weakness, walking
difficulties, tremors, vision problems, poor immune function, and
numbness.
Vitamin K
Vitamin K is a fat-soluble vitamin that belongs to the family of
compounds, including phylloquinone (vitamin K1) and a series of
menaquinones (vitamin K2).
The main dietary forms are vitamin K1(phylloquinone), found in
plant foods, and vitamin K2(menaquinones), found in
animal-derived foods and fermented soy products.
Functions of Vitamin K
Vitamin K plays an essential role in blood clotting. In fact, the "K"
stands for "koagulation," the Danish word for coagulation, which
means clotting.
Other functions of vitamin K include supporting bone health and
helping prevent the calcification of blood vessels, potentially
reducing the risk of heart disease.
Vitamin K Food Sources
63
64
M3: Micronutrients
L 2: Water-Soluble Vitamins: B1, B2, B3, B5, B6
65
66
The most common disease of thiamin deficiency is beriberi.
Beriberi causes impaired sensory, motor, and reflex functions.
Therapeutic doses of vitamin B1 can help overcome beriberi.
Vitamin B2
Vitamin B2, also known as riboflavin, is a water-soluble vitamin.
Our bodies can only store very little of the water-soluble vitamins,
usually in the liver, heart, and kidneys, and the excess is excreted
via urine.
Functions of Vitamin B2
Vitamin B2 is responsible for maintaining healthy blood cells. It
helps in boosting energy, protecting skin and eye health.
A point to note is that literally, all B vitamins help extract energy
from the food you eat into usable energy that is in the form of ATP.
Vitamin B2 Food Sources
Eggs, milk, organ meats have the highest content of vitamin B2.
Spinach, almonds, mushrooms are some plant-based sources of
this vitamin.
Like vitamin B1, B2 is water-soluble and is lost in the cooking
water. To minimize loss, it’s best to steam rice and not overcook
your food.
Vitamin B2 Deficiency
67
68
69
70
M3: Micronutrients
L 3: Water-Soluble Vitamins: B7, B9, B12, C
V itamin B7
71
72
73
Vitamin B12 Functions
Vitamin B12 is responsible for making red blood cells. It is also
involved in DNA formation and regulation.
Vitamin B12 helps prevent megaloblastic anemia, a disorder in
which red blood cells become larger than normal, immature, and
few, which reduces the amount of oxygen carried by the blood to
the tissues. 45
Vitamin B12 is also a crucial nutrient to maintain healthy eyes,
brain, heart, hair, skin, and nails.
Food Sources
Vitamin B12 is mostly found in animal foods. The top sources are
fish, meat, eggs, and milk. 46
Nutritional yeast and fortified foods like bread and cereals have
this vitamin.
Vitamin B12 Deficiency
An unfortunate fact is that large amounts of folic acid (vitamin B9)
can mask vitamin B12 and could result in some severe
neurological damage when not treated for a few years.
Additional vitamin B12 deficiency symptoms include poor memory,
dementia, depression, confusion, megaloblastic anemia, weakness,
constipation, and weight loss.
74
75
To ensure a high supply of vitamin C daily, it's a good idea to eat at
least five servings of fresh fruits and vegetables.
Vitamin C Deficiency
Scurvy is a disease that may start to manifest if the vitamin C
intake falls below 10mg/day for weeks.
Inflammation in the gums and malaise are the initial symptoms,
and if left untreated, it leads to scurvy.
As this disease progresses, joint pain, swollen gums, loosening, or
loss of teeth can happen. If scurvy is left untreated, then it could
prove fatal. Vitamin C deficiency is rare in the developed world.
In summary, you can see that deficiency of both fat-soluble and
water-soluble vitamins is rare and limited to people who have
specific diseases or some disorder, which reduces the body's
ability to absorb these critical vitamins.
Lastly, vitamin deficiency is more in developing nations where
there is widespread malnutrition.
Lets now discuss minerals starting with the next lecture.
76
M3: Micronutrients
L 4: Macro-Minerals: Calcium, Phosphorus,
Magnesium
Your body uses minerals for many different jobs, including keeping
your bones, muscles, heart, and brain working properly.
Minerals are also important for making enzymes and hormones.
There are macro minerals needed by the human body in large
quantities, and then there are trace minerals required by our body
in small amounts.
The macrominerals are calcium, phosphorus, magnesium, sulfur,
sodium, chloride, and potassium. The amounts needed are more
than 100 milligrams (mg) per day.
Calcium
Calcium is the most abundant mineral in the body. Calcium is
mostly found in bones, and it constitutes 2% of your total body
weight.
Functions of Calcium
77
Since most of the calcium is found in our bones, low calcium intake
may result in bone resorption.
It's important to know that our bones are constantly remodeling.
In children and adolescents, bone formation exceeds resorption as
the human body is growing at that time.
However, as we start to age, bone resorption exceeds formation,
which is especially true for postmenopausal women who have to
take extra precautions when it comes to calcium intake to avoid
osteoporosis.
Osteoporosis is a skeletal disorder in which bone mass and bone
strength are affected. 49
Adequate calcium and vitamin D, along with physical activity, may
reduce the risk of osteoporosis. 50
Calcium is also needed to regulate heart rhythms, aids in muscle
function, and regulate blood pressure and cholesterol levels.
Calcium Food Sources
Milk and milk products like yogurt, cheese have the highest
concentrations of calcium.
Green leafy vegetables like spinach, kale, and broccoli have some
calcium, but the bioavailability decreases because of anti-nutrients
like oxalic acid.
78
Calcium Deficiency
In the short term, there is no calcium deficiency, as blood calcium
levels are tightly regulated. But in the long run, it may bone mass
and growth, especially in children and postmenopausal women.
Vegans or people with lactose intolerance are also at risk of
calcium deficiency.
Calcium deficiency eventually can lead to bone fractures and
osteoporosis.
Phosphorus
Phosphorus is a mineral that makes up 1% of a person's total body
weight. It is the second most available mineral in the body after
calcium.
Most of the body's phosphorus is in the bones, teeth, DNA, and
RNA.
Functions of Phosphorus
Phosphorus and calcium play a significant role in the creation of
bones and teeth. 51
Phosphorus exists in every cell of the human body and contributes
to RNA and DNA formation, responsible for the storage and
transmission of genetic information. 52
79
80
81
M3: Micronutrients
L 5: Macro-Minerals: Sodium, Chloride,
Potassium, Sulphur
in salt.
Sodium is found mostly in blood plasma and the extracellular fluid.
The body obtains sodium through food and drink and loses it
primarily in sweat and urine.
Most of the sodium we consume is salt, sodium chloride-40%
sodium, and 60%chloride.
Functions of Sodium
Sodium is an electrolyte in the body fluids like the blood that carry
an electric charge. Sodium and other minerals like potassium help
the body keep fluids in a healthy balance.
Helps with Nerve and Muscle Function
The heart, muscle, and nerve cells use electrolytes like sodium to
carry electrical impulses to other cells. 56
82
Sodium Recommended Intake
The Daily Value (DV) for sodium is less than 2,300 milligrams (mg)
per day.
But due to a significant portion of calories coming from processed
foods (high in sodium), the average American consumes around
3,400mg of sodium per day.
Sodium F ood Sources
You don't have to worry about getting enough sodium in your diet.
If most of your calories come from processed foods, you should be
more worried about how you can lower your sodium intake!
Monosodium glutamate(MSG), baking soda, sodium nitrite, sodium
saccharin, and sodium benzoate are different sodium forms and
added to many food products. These increase the flavor, texture,
appearance, and shelf life of the foods.
It is good to eat most of your calories from home-made foods
prepared from fresh, local, and seasonal ingredients for healthy
sodium intake.
Sodium D eficiency
It's extremely rare to run low on sodium as most people eat
significant calories from processed foods!
83
84
85
86
Potassium Food Sources
Potassium is available from a variety of vegetables, fruits, dairy,
nuts, and unrefined foods.
Lentils and beans are also high in this essential mineral.
Potassium Deficiency
Hypokalemia ( low potassium level) occurs in the body due to
excessive urination because of prescription medications, severe
sweating, vomiting, overuse of laxatives, kidney disease, and
genetic disorders. 59
Mild hypokalemia symptoms are constipation, fatigue, and muscle
weakness.
However, potassium deficiency is very rare, especially in people
with healthy kidney function.
Sulphur
Sulfur is an element that exists in nature and is freely available in
soil, plants, foods, and water.
Sulfur is supplied mainly by the intake of two amino acids,
methionine, and cysteine, found in plant and animal proteins.
Sulfur becomes an essential constituent of amino acids, proteins,
enzymes, vitamins, and other biomolecules.
Function of Sulphur
87
88
89
M3: Micronutrients
Microminerals.
Even though our bodies need microminerals in minimal quantities
(0.2 to 15mg/day), they are still essential to our diet daily.
There are nine microminerals: Iron, Zinc, Copper, Manganese,
Selenium, Chromium, Iodine, Molybdenum, and Cobalt.
Let's start with iron.
Iron
Iron is a micromineral essential for all living organisms and found
in every cell of the body.
Functions of Iron
It plays a role in metabolic processes, including oxygen transport,
deoxyribonucleic acid (DNA) synthesis, electron transport, and
hormones synthesis.
90
91
92
Vegetarians may need more Zinc than the recommended due to
the low bioavailability of Zinc from plant-based foods.
Zinc Deficiency
Low dietary intake, malabsorption, chronic illnesses like diabetes,
cancer, and liver diseases can create Zinc deficiency.
Symptoms are loss of appetite, frequent infections, retarded
growth, and anemia. In severe cases, it can result in hair loss, loss
of sense of taste, and smell. 65
Low levels of Zinc are evident in vegetarians, pregnant and
lactating women, and anyone suffering from gastrointestinal
diseases.
Copper
Copper is an essential trace mineral and vital to the body's health
from fetal development to old age.
Copper in the body is located in the liver, bones, and muscle, but
copper traces occur in all body tissues.
Most of the copper is excreted in bile and a small amount in urine.
Functions of Copper
The body uses copper to carry out many vital functions, including
making energy, and maintaining the nervous and immune system.
93
Your body also needs copper for brain development and iron
absorption.
Copper plays a vital role in our metabolism, primarily because it
allows many critical enzymes to function correctly.
Copper is necessary for the maintenance of a healthy white blood
cell count.
Too little copper can lead to neutropenia, a deficiency of white
blood cells, or neutrophils, which fight off infection.
Copper Food Sources
Animal sources are shellfish, oysters, crab, and organ meat.
Plant sources are whole grains, beans, dark leafy greens, potato,
and chocolate.
Seeds and nuts rich in copper are cashews, hazelnut, sunflower
seeds.
Copper D eficiency
Copper deficiency can result from malnutrition, malabsorption, or
excessive zinc intake and can be acquired or inherited.
The symptoms are low white blood cells with increased risk of
infection, fatigue, anemia, osteoporosis, Hypopigmentation,
connective tissue disorder, nerve damage, and muscle weakness.
66
94
Manganese
Manganese is a trace mineral, vital but required in a small
quantity.
Manganese is ingested through the gastrointestinal tract and
stored in the liver, pancreas, bones, kidneys, and brain.
Functions of Manganese
Manganese is essential for forming healthy cartilage and bone for
blood clotting and helps in wound healing.
It plays a role in numerous chemical processes, including the
synthesis of nutrients like cholesterol, carbohydrates, and
proteins.67
Manganese Food Sources
Plant-based foods rich in manganese are sprouted grains,
legumes, beans, nuts, and seeds.
Animal sources are clams, oysters, and mussels.
Manganese Deficiency
Manganese deficiency is rare and usually limited to people
suffering from medical issues like diabetes, osteoporosis, epilepsy
Signs of a potential manganese deficiency may include impaired
growth, impaired reproductive system, and skeletal system
abnormalities. 68
95
96
M3: Micronutrients
L 7: Macro-Minerals: Selenium, Chromium,
Iodine, Molybdenum, Cobalt
S elenium
97
Plant sources are brazil nuts, beans, lentils, grains, green
vegetables, bread, and dairy.
Selenium Deficiency
Selenium deficiency is rare; however, if manifested may cause
poor immune function, cognitive decline. 71
Chromium
Chromium is a naturally occurring element found in rocks,
animals, plants, soil, and volcanic dust and gases.
Dietary Chromium is poorly absorbed, and levels of absorption
decrease with age.
Only a small amount of the Chromium from food is absorbed, and
the absorption is better if eaten with foods containing vitamin C
and niacin.
Functions of Chromium
Chromium is an essential mineral that has a beneficial role in
regulating insulin action and its effects on carbohydrate, protein,
and lipid metabolism. 72, 73
It stimulates fatty acid and cholesterol synthesis, which is vital for
brain function.
Chromium Food Sources
98
99
About 70-80% of Iodine is in the thyroid gland in the neck, the rest
is in the blood, muscles, ovaries, and other parts of the body.
Functions of Iodine
Iodine's principal physiological role in the human body is to
produce the Thyroid-stimulating hormone( THS) known as
thyrotropin.
Thyroid hormones regulate crucial biochemical reactions, including
protein synthesis and enzymatic activity, and are critical
determinants of metabolic activity.
Iodine is an essential component of the thyroid hormones
triiodothyronine (T3) and thyroxine (T4) and vital for healthy
thyroid function.
All cells in our body depend on T3 and T4 for the regulation of
their metabolism.
Iodine Food Sources
Seafood is abundant in Iodine because marine animals absorb
some of the Iodine that is naturally present in seawater.
Certain types of edible seaweed such as wakame, nori, and kombu
are also rich in Iodine.
Eggs, fruit, vegetables, grain products, dairy, and poultry are good
sources of Iodine, but the amount varies on the soil and fertilizer's
iodine content.
100
101
102
Functions of Cobalt
Cobalt plays a significant role in forming amino acids and some
proteins in nerve cells and creating neurotransmitters that are
indispensable for the organism's correct functioning.
Cobalt is also part of the biotin-dependent Krebs-cycle, which the
body uses to break down sugars into energy.
Cobalt Food sources
Animal sources rich in cobalt are fish, eggs, organ meat, muscle
meat, poultry, and milk.
Plant sources are Nuts, seeds, green leafy vegetables, broccoli,
peas, beans, and root vegetables.
Cobalt Deficiency
Cobalt deficiency is strongly related to disturbances in vitamin B12
synthesis.77
Cobalt deficiency can cause pernicious anemia. The symptoms can
include numbness, fatigue, tingling in your hand and feet, nausea,
appetite loss, difficulty maintaining balance, weight loss, and
headache.
103
Types of Fiber
Soluble Fiber
Food sources rich in soluble fiber are peas, oats, apples, beans,
carrots and barley.
The main benefits of soluble fiber are that it helps to reduce blood
glucose levels and blood cholesterol levels.
104
Insoluble Fiber
A high fiber diet is more filling as it helps stabilize your blood sugar
levels which significantly reduces your cravings and hunger.
105
This is because cravings are simply a cry from your body to ingest
more micronutrients that it needs to deal with billions of chemical
reactions happening in your body at any given point in time.
106
It’s important to eat a variety of high fiber foods to ensure that you
get both types of fiber i.e. soluble and insoluble.
Just focus on including the following foods that I have shown in the
table on a daily basis. This will ensure that you reach the optimal
fiber intake according to your age and gender.
107
Oats, barely, ragi, brown rice, roti are all high fiber foods. Try and
replace white bread, rice with whole wheat bread and brown rice
respectively.
Lastly, if you eat meat almost daily, starting including lentils and
beans in your diet a few times a week. This is because meats have
zero fiber in them and lentils and beans have a lot in them!
108
L 2: Probiotics
spinal cord or the peripheral nervous system and that's the very
reason scientists call it the second brain.
Roughly 70% of our immune system is located within the gut in the
form of gut-associated lymphoid tissue [GALT] 83
Literally there are more immune cells in your gut than anywhere in
your body.
Another amazing fact is that more than 90% of the ‘feel good’
hormone serotonin is produced in your gut.
diseases, right?
109
Not really!
Quite simply, bacteria in your gut is divided into good and bad
Bad bacteria are the kind that multiply and mean harm. Some
Probiotics are simply the good bacteria in your gut, including some
yeast that can act as probiotics.
This makes a lot of sense since the foods we eat and drink are
loaded with pathogens, viruses and bacteria.
Having our immune system in the gut allows it to fight off these
unwanted invaders more effectively.
110
Dopamine. 85,
86
The gut is the largest site for serotonin as gut microbes and gut
cells produce it.
Probiotics help fend off bad bacteria that try to harm you.
If this ratio is disrupted due to changes in diet and lifestyle like less
sleep, more stress, high intake of alcohol, smoking, antibiotic use,
consumption of fast and junk food, consumption of conventional
dairy, etc., then symptoms like headaches, mood swings, weight
gain, acne, gas, bloating, and stomach pain start to crop up.
111
112
L 3: Water
Before I discuss how much water one should consume daily, let’s
understand the key role in the human body.
113
According to BrainMD.Com:
“Your brain is 80% water, your muscles (including your heart) are
75% water, your blood is 83% water, your lungs are 90% water,
your skin is 64% water, and even your bones are 30% water.”
To curb their cravings, I suggest that they include water as the first
thing they should reach for.
114
It’s important to note that these beverages are 99% water and is a
very effective strategy to curb cravings and act as appetite
suppressants.
Various other studies support the same results that water intake
results in an increased metabolic rate.90, 91
115
However, when you drink enough water, there is no reason for the
body to retain water!
This reduces the ‘puffy’ look and makes you look toned.
Just like one’s protein intake is intrinsically linked with their activity
levels, the same way your water intake will vary based on where
you live and how active you are.
If you live in a country where it’s hot and humid most of the time,
your water intake will be higher because you will lose more water
in sweat.
116
For example, if you eat a 2,000 calorie diet, you should drink 2
liters of water per day.
When you average the intake touted by most health authorities, it
comes to roughly ~2 liters per day.
Most fruits and vegetables are 75-99% water. Meats, poultry are
50-65% water.
117
The best way to ensure optimal water intake is to let your body be
your guide.
For starters, ensure that your urine color is always light yellow in
color or colorless.
Also, please do note that the moment you start to feel thirsty, you
are already dehydrated. So it’s a good idea to drink water
throughout the day.
118
L 1: N
utrition Myth No # 1: Egg Yolks are Bad For
You, They Cause Cholesterol Issues
to use hard science to bust top 7 nutrition myths that just refuse to
go away.
Let's start with the first nutrition myth and that is:
Nutrition Myth No # 1 Egg Yolks are bad for you they cause
cholesterol issues
119
One medium egg has about 185mg cholesterol and if you eat two
eggs you have already crossed that limit!
So, eating less or no eggs (or just the white portion) will not cause
increased cholesterol and you will largely reduce the chances of
having coronary heart disease.
Right?
First some anecdotal experience: I have been eating eggs for many
decades now.
What has allowed me to fearlessly eat these many eggs every day
when everyone around me is throwing away the yolk or even
completely replacing omelets with cornflakes?
120
In the end, the decision lies with you, my job is to educate and
empower and not force you into making any decision.
Just think, why would your body manufacture something that will
harm itself?
If you eat more cholesterol from dietary sources like eggs, your
body produces less of it. In the end, your body has the innate
wisdom to balance out its cholesterol levels based on your level of
dietary consumption.
121
#1 High-Quality Protein
122
One special nutrient with which whole eggs are loaded with is
choline.
Eating eggs almost every day also makes counting calories very
simple, and we all know how important counting calories is for fat
loss or muscle gain.
123
Lutein and Zeaxanthin are antioxidants that are very beneficial for
eye health.
Besides eating eggs, these two nutrients are abundantly found in
kale, spinach, collards, turnip greens, green peas, broccoli.
124
125
BMR is the calories you burn at complete rest, like lying down on a
sofa the whole day, doing nothing.
Now, let's calculate the thermic effect of food. The formula for TEF
is 10% of your BMR.
When you eat food, some energy is required to digest and process
that food. This is called the thermic effect of food.
126
Firstly, when compared with the BMR, the thermic effect of food is
not that significant.
Even when compared with your activity level it is not that much.
The bottom line is that food has very little to do with your
metabolism and the major players are your BMR and your activity
level. Always remember this.
127
“W hen saturated fat got mixed up with the high sugar
added to processed food in the second half of the 20th century, it
got a bad name,” noted UC San Francisco pediatric endocrinologist
Robert Lustig.
It all started with Ancel Keys and the Seven Countries Study. Keys
launched the Seven Countries Study in 1958 to research the
relationship between dietary patterns and the prevalence of
coronary heart disease. He uncovered a direct link between heart
disease from high total serum cholesterol and saturated fat intake.
However, the study was seriously flawed; Keys started out with
data from 22 countries and simply omitted the data from the
countries that didn’t fit with his hypothesis!
For the last four decades, because of him and his deceptive study,
we have been eating cereals with skimmed milk instead of whole
egg omelets made in butter.
128
They state that saturated fats raise the level of cholesterol in your
blood but fail to delineate which type of cholesterol they are
referring to!
On the contrary, I feel saturated fats are protective and found in
real food and not junk.
129
“The mantra that saturated fat must be removed to reduce the risk
of cardiovascular disease has dominated dietary advice and
guidelines for almost four decades. Yet scientific evidence shows
that this advice has, paradoxically, increased our cardiovascular
risks. 106
130
Nature cannot be wrong. Did you know that mother’s milk has
more than 50 percent of calories from saturated fat and a higher
proportion of cholesterol? This is because cholesterol and
saturated fat are essential for growth in babies and children,
especially when it comes to brain development.
Saturated fats are very stable and do not oxidize when used in
cooking at high temperatures. However, the oils most of us tend to
use are polyunsaturated (canola, soy, corn, safflower, and
sunflower). These do oxidize when they are used for high heat
cooking, creating harmful free radicals in the process.
Saturated fats play many vital roles in the body, such as helping to
strengthen the immune system, promote healthy bones, protect
the liver, and provide structural integrity to the cells. After all,
saturated fatty acids make up at least half of your cell membranes.
131
I drink whole milk, and I cook in desi ghee (butter) and extra virgin
cold-pressed coconut oil all the time.
132
Visualize this situation when food was scarce and agriculture was
not invented.
Of course not, they would eat until their heart desired— because
they were not sure where their next meal would come from.
133
I have a feeling that this myth that you cannot absorb more than
30g of protein in one sitting was started by supplement
companies.
134
In case you are not gaining weight, put an alarm at 3 am, and
squeeze in one serving then. It will help you gain weight.
So coming back to the point, can the human body really absorb
30g of protein in one sitting?
Yes, it can!
Your body is very smart and intelligent and can easily self-regulate
its digestion.
If you eat more protein in one sitting, as I do, and millions of
others like me, our bodies just take a longer time to absorb it.
135
At the same time, if you have crossed your TDEE and your body
has utilized the protein, anything excess will likely be converted
into body fat.
136
for protein intake is less than the amount actually required for
active individuals and should thus be adjusted according to one’s
type of activity as explained above.
137
138
139
compared with diets that are high in carb, thus leading to more
weight loss in the long term.110
140
coconut oil containing saturated fats are unhealthy and raise the
risk of heart disease and cardiovascular problems.
We have already busted the myths about saturated fats and why
consuming them is NOT dangerous.
141
For starters, do note that if the vegetable oils contain the word
'vegetable' in them that does not mean they are unhealthy.
In fact, it's the exact opposite. Vegetable oils are very unstable at
high temperatures and grow rancid when used for cooking.
Why?
If you are thinking what's the big deal with inflammation, well let
me be quick to point out that inflammation is a leading cause of a
host of health issues including heart disease, metabolic syndrome,
diabetes, arthritis, Alzheimer's, many types of cancer, etc.
142
Bottom line, you want the ratio of omega 3: omega 6 1:1 or ideally
very close to this ratio.
Before you start thinking about how to get enough omega 3 in
your diet, doesn't it make sense to first reduce omega 6 in your
diet?
Then let's see how you can increase omega 3 content in your diet:
If you are a fish eater, then focus on eating fatty fishes like salmon
and tuna. A quick word of warning.
So try and focus on wild water fishes and try to avoid larger fishes
like shark, swordfish, and mackerel, which have the highest
amounts of contaminants.
Instead, you should focus on eating shrimp, light tuna, and catfish,
and of course salmon.
143
I agree walnuts have ALA but the body requires Omega 3 in the
form of DHA and EPA and humans are very poor at converting ALA
to EPA and DHA.
So, guys, omega 3 supplementing with krill oil for the majority of
you is a very safe bet and can dramatically improve your health.
Vegans and vegetarians who cannot stand the smell of fish oils or
even krill oil (the latter has little or no smell by the way) flaxseed oil
will provide you with enough omega 3 for your daily needs!
Ok, this is the end of this module and the next module we’ll
discuss 5 nutrition facts. Thanks for listening and see you in the
next module.
144
L 1: D
iets fail. what you need is a lifestyle change
According to New York Times, ninety-five percent of people who
lose weight on a diet gain it back, and a significant percentage of
people gain back more than they had originally lost, just like in less
than a year or two almost everyone who wins the lottery is worse
off than before they won it! 113
Moreover, ninety-two percent of people give up their New Year
Resolutions of losing weight and becoming healthier!
These statistics are dismal.
Instead, what is required is a lifestyle change.
You see, most diets are restrictive and inflexible. This makes them
not sustainable.
They believe that to lose weight, it's normal to give up their
favorite foods altogether, eat a severely calorie restrictive diet, and
focus on certain so-called 'fat loss' foods that can help lose the
flab.
145
What happens after a month or so: they do indeed lose a lot of
weight but at a cost.
Because they were under a severe caloric restriction, two things
happened: They lose a lot of lean muscle, as the weighing scale
was going down effortlessly without exercise, and they think there
is no need to work out.
Anyways, it's not easy to work out by eating fewer calories.
Because of calorie deprivation, their metabolism shuts down, and
cravings and hunger start to build up. Not good.
And now that they have managed to lose a lot of weight (even
though they don't look healthy, toned, or sexy as without gaining
any lean muscle, you really cannot shape your body), they still
think it's a victory and deserve a treat.
The treat would not be broccoli.Instead, they go out with their
friends and family and attack on a delicious pizza, a medium coke,
and large fries.
At this time, their body's metabolism is damaged and is primed to
gain fat.
Most of the calories will go towards fat storage.
When you deprive and restrict food for that long, the body thinks a
famine is coming, and it's better to store calories in case it hits
again.
146
Excellent, now all 1000 + calories you had in your pizza and coke
and fries goes towards your gut, all fat.
This cycle repeats itself. You start to feel guilty, craving increasing
as its a cry of nutrients required by your body, but junk and fast
food has only empty calories in them,
And the facts that I mentioned at the start comes true, the majority
of people who lose weight on a diet gain it all back within a year of
dieting, and most of them gain back more than they had originally
lost.
147
L 2: R
efined carbohydrates and added sugar are
bad for you
148
149
L 3: S
upplements can never completely replace
your diet
way it's grown, the way it is transported, and the way we store it
and cook it.
Unless you are buying your produce from a local farmers market
and taking every precaution to store it effectively and consume it
by cooking on medium heat and not overcooking it, there is almost
a loss of close to 50-70% loss of nutrients in your food.
There is another issue. 60% of the calories come from processed
foods for Americans. The reality is no different than in the rest of
the world.
Factory foods are highly processed and offer very little or no
nutritional value.
It's straightforward.
150
151
L 4: E
ating more fruits and vegetables is good for
your health.
As we discovered in Module titled: Micronutrients, students just
how important it is to get all the essential vitamins and trace
minerals as the human body cannot manufacture them
independently.
You realize how vital they are and the critical role they play in
keeping us alive.
Fruits and vegetables are the single most significant source of
these micronutrients.
Now, I get it; it's hard work to consume 5-8 servings of fresh fruits
and vegetables every day!
To go to the grocery store, buy the fruits and vegetables, which are
more expensive than an Mc Donald burger, and then they don't
even taste that good.
Students, it's a matter of developing a perspective and a long-term
mindset.
152
Fruits and vegetables are important, and if you eat enough every
day, you are giving the body nutrients that will keep you optimally
healthy. You have to do the work and believe that it will pay off in
the long term. That's the only way.
Being good at making salads makes it a habit to include some
veggies with every meal you eat, add various colors of veggies, and
green leafy is the most important.
Try and ferment your vegetables for probiotics. And needless to
say, veggies are also the most significant source of fiber.
Fibre and probiotics are two nutrients that most people are
severely deficient in.
153
L 5: T
here is no perfect diet for everyone
We all thrive on different foods; we eat foods based on our culture,
upbringing, preference, and lifestyle.
Just because the keto diet is a rave now does not mean you will
also get great results. You may or may not.
A cookie-cutter diet plan may not work for you.
You need to find or create a diet for yourself and your clients (if
you plan to become a nutrition coach) according to their unique
needs, including their culture, preferences, and overall lifestyle.
Do check out an article titled: Best Diet, which you can find on my
website, to know more about this topic.
I will be coming out with an in-depth course titled: Holistic
Nutrition Coach Certification. That course is specifically for
individuals who want to build their part-time/full-time career in the
vast field of Nutrition as nutrition coaches. I cannot promise an
154
exact date, but it should be launched in the first half of 2021; stay
tuned.
Students, this is the end of this fundamental diploma in Nutrition.
There is still a lot of bonus content for you to check out to deepen
your nutrition knowledge further.
If you still haven't downloaded your master and resource manuals,
please do. I urge you to listen to the lectures again before you
attempt your final exam.
I hope you graduate with flying colors.
I thank you once again from the bottom of my heart for placing
your trust in me and supporting me in my mission by buying this
diploma course from my Academy.
I wish you all the best for your success. Lots of love and take care.
155
References
1) Mohammad Ishraq Zafar, Kerry E Mills, Juan Zheng, Anita Regmi, Sheng Qing Hu,
Luoning Gou, Lu-Lu Chen, Low-glycemic index diets as an intervention for diabetes:
a systematic review and meta-analysis, The American Journal of Clinical Nutrition,
Volume 110, Issue 4, October 2019, Pages 891–902,
https://doi.org/10.1093/ajcn/nqz149
2) Sylvia H. Ley, PhD, RD,a Osama Hamdy, MD, PhD,b V. Mohan, MD, PhD,c and Frank
B. Hu, MD, PhDa,d, Prevention and Management of Type 2 Diabetes: Dietary
Components and Nutritional Strategies, PMID: 24910231, Lancet 2014 Jun
7;383(9933):1999-2007. doi: 10.1016/S0140-6736(14)60613-9
https://pubmed.ncbi.nlm.nih.gov/24910231/
3) Aundrea Parker 1 , Yeonsoo Kim 1, The Effect of Low Glycemic Index and Glycemic
Load Diets on Hepatic Fat Mass, Insulin Resistance, and Blood Lipid Panels in
Individuals with Nonalcoholic, Fatty Liver Disease, PMID: 31305201 DOI:
10.1089/met.2019.0038, https://pubmed.ncbi.nlm.nih.gov/31305201/
5) Robert R. Wolfe, Branched-chain amino acids and muscle protein synthesis in
humans: myth or reality?, Journal of the International Society of Sports Nutrition,
Published: 22 August 2017
https://jissn.biomedcentral.com/articles/10.1186/s12970-017-0184-9
156
8) Dietary Reference Intakes for Energy, Carbohydrate, Fiber, Fat, Fatty Acids,
Cholesterol, Protein, and Amino Acids (2005), Chapter: 10 Protein and Amino Acids,
The National Acadamies Press https://www.nap.edu/read/10490/chapter/12
9) D Travis Thomas, Kelly Anne Erdman, Louise M Burke, American College of Sports
Medicine Joint Position Statement. Nutrition and Athletic Performance, PMID:
26891166 DOI: 10.1249/MSS.0000000000000852
https://www.ncbi.nlm.nih.gov/pubmed/26891166
10) Richard B Kreider 1, Bill Campbell, Protein for exercise and recovery, PMID:
20048505 DOI: 10.3810/psm.2009.06.1705
https://pubmed.ncbi.nlm.nih.gov/20048505/
11) Ralf Jäger, Chad M Kerksick, Bill I Campbell, Paul J Cribb, Shawn D Wells, Tim M
Skwiat, Martin Purpura, Tim N Ziegenfuss, Arny A Ferrando, Shawn M Arent, Abbie E
Smith-Ryan, Jeffrey R Stout, Paul J Arciero, Michael J Ormsbee, Lem W Taylor, Colin D
Wilborn, Doug S Kalman, Richard B Kreider, Darryn S Willoughby, Jay R Hoffman,
Jamie L Krzykowski, Jose Antonio, International Society of Sports Nutrition Position
Stand: protein and exercise, PMID: 28642676 PMCID: PMC5477153 DOI:
10.1186/s12970-017-0177-8, https://pubmed.ncbi.nlm.nih.gov/28642676/
12) Robert W Morton, Kevin T Murphy, Sean R McKellar, Brad J Schoenfeld, Menno
Henselmans, Eric Helms, Alan A Aragon, Michaela C Devries, Laura Banfield, James
W Krieger, Stuart M Phillips, A systematic review, meta-analysis and meta-regression
of the effect of protein supplementation on resistance training-induced gains in
muscle mass and strength in healthy adults, PMID: 28698222 PMCID: PMC5867436
DOI: 10.1136/bjsports-2017-097608, h ttps://pubmed.ncbi.nlm.nih.gov/28698222/
13) Jay R Hoffman, corresponding author: Nicholas A Ratamess, Jie Kang, Michael J Falvo
and Avery D Faigenbaum, Effect of Protein Intake on Strength, Body Composition
and Endocrine Changes in Strength/Power Athletes, J Int Soc Sports Nutr. 2006; 3(2):
12–18,Published online 2006 Dec 13,
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2129168
14) Philip C Calder, Functional Roles of Fatty Acids and Their Effects on Human Health,
PMID: 26177664 DOI: 10.1177/0148607115595980,
https://pubmed.ncbi.nlm.nih.gov/26177664/
157
16) Patty W Siri-Tarino, Qi Sun, Frank B Hu, and Ronald M Krausscorresponding author,
Meta-analysis of prospective cohort studies evaluating the association of saturated
fat with cardiovascular disease, PMID: 20071648, Published online 2010 Jan 13,
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2824152/
17) Huabing Zhang 1, Jorge Plutzky, Stephen Skentzos, Fritha Morrison, Perry Mar, Maria
Shubina, Alexander Turchin, Discontinuation of statins in routine care settings: a
cohort study, PMID: 23546564 PMCID: PMC3692286 DOI:
10.7326/0003-4819-158-7-201304020-00004,
https://pubmed.ncbi.nlm.nih.gov/23546564/
18) Giuseppe Querques, * Raimondo Forte, and Eric H. Souied, Retina and Omega-3, J
Nutr Metab. 2011; 2011: 748361., Published online 2011 Oct 31. doi:
10.1155/2011/748361,
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3206354/#:~:text=The%20retina%20
has%20a%20high,antiapoptotic%2C%20role%20in%20the%20retina
21) Mimi Chiu, Alexander Dillon, Stephanie Watson, Vitamin A deficiency and
xerophthalmia in children of a developed country, PMID: 27439630 DOI:
10.1111/jpc.13243, https://pubmed.ncbi.nlm.nih.gov/27439630/
22) James Cheshire, Sai Kolli, Vitamin A deficiency due to chronic malabsorption: an
ophthalmic manifestation of a systemic condition, PMID: 28490481 PMCID:
PMC5614218 DOI: 10.1136/bcr-2017-220024,
https://pubmed.ncbi.nlm.nih.gov/28490481/
23) Elina Manusevich Wiseman, Shimrit Bar-El Dadon, Ram Reifen, The vicious cycle of
vitamin a deficiency: A review, PMID: 27128154 DOI:
10.1080/10408398.2016.1160362, h ttps://pubmed.ncbi.nlm.nih.gov/27128154/
24) Silvia Martini, Angela Rizzello, Ilaria Corsini, Benedetta Romanin, Michelangelo
Fiorentino, Sara Grandi, Rosalba Bergamaschi, Vitamin A Deficiency Due to Selective
Eating as a Cause of Blindness in a High-Income Setting , PMID: 29610168 DOI:
10.1542/peds.2016-2628, h ttps://pubmed.ncbi.nlm.nih.gov/29610168/
158
26) Anna Piotrowska, Justyna Wierzbicka, Michał A Żmijewski, Vitamin D in the skin
physiology and pathology, PMID: 26824295 DOI: 10.18388/abp.2015_1104,
https://pubmed.ncbi.nlm.nih.gov/26824295/
27) Ruoxu Dou , Kimmie Ng, Edward L Giovannucci, JoAnn E Manson, Zhi Rong Qian,
Shuji Ogino, Vitamin D and colorectal cancer: molecular, epidemiological and clinical
evidence, PMID: 27245104 PMCID: PMC4890569 DOI: 10.1017/S0007114516000696,
https://pubmed.ncbi.nlm.nih.gov/27245104/
28) Fatemeh Khadangi, Angelo Azzi, Vitamin E - The Next 100 Years, PMID: 30550633
DOI: 10.1002/iub.1990, https://pubmed.ncbi.nlm.nih.gov/30550633/
30) Solmaz Akbari, Amir Alireza Rasouli-Ghahroudi, Vitamin K and Bone Metabolism: A
Review of the Latest Evidence in Preclinical Studies, PMID: 30050932 PMCID:
PMC6040265 DOI: 10.1155/2018/4629383,
https://pubmed.ncbi.nlm.nih.gov/30050932/
32) Gary E Gibson, Joseph A Hirsch, Pasquale Fonzetti, Barry D Jordan, Rosanna T Cirio ,
Jessica Elder, Vitamin B1 (thiamine) and dementia, PMID: 26971083 PMCID:
PMC4846521 DOI: 10.1111/nyas.13031,
https://pubmed.ncbi.nlm.nih.gov/26971083/
35) Donald L Bissett, John E Oblong, Cynthia A Berge, Niacinamide: A B vitamin that
improves aging facial skin appearance, PMID: 16029679 DOI:
10.1111/j.1524-4725.2005.31732, h ttps://pubmed.ncbi.nlm.nih.gov/16029679/
36) Rosemary A Fricker, Emma L Green, Stuart I Jenkins, Síle M Griffin, The Influence of
Nicotinamide on Health and Disease in the Central Nervous System, PMID:
29844677 PMCID: PMC5966847 DOI: 10.1177/1178646918776658,
https://pubmed.ncbi.nlm.nih.gov/29844677/
159
37) Malkanthi Evans , John A Rumberger, Isao Azumano , Joseph J Napolitano, Danielle
Citrolo , Toshikazu Kamiya, Pantethine, a derivative of vitamin B5, favorably alters
total, LDL and non-HDL cholesterol in low to moderate cardiovascular risk subjects
eligible for statin therapy: a triple-blinded placebo and diet-controlled investigation,
PMID: 24600231 PMCID: PMC3942300 DOI: 10.2147/VHRM.S57116,
https://pubmed.ncbi.nlm.nih.gov/24600231/
39) L C Rall, S N Meydani, Vitamin B6 and immune competence, PMID: 8302491 DOI:
10.1111/j.1753-4887.1993.tb03109.x, h ttps://pubmed.ncbi.nlm.nih.gov/8302491/
41) Hamid M Said, Biotin: biochemical, physiological and clinical aspects, PMID:
22116691 DOI: 10.1007/978-94-007-2199-9_1,
https://pubmed.ncbi.nlm.nih.gov/22116691/
42) Shari R Lipner, Richard K Scher, Biotin for the treatment of nail disease: what is the
evidence?, PMID: 29057689 DOI: 10.1080/09546634.2017.1395799,
https://pubmed.ncbi.nlm.nih.gov/29057689/
43) Nassim Naderi, James D House, Recent Developments in Folate Nutrition, PMID:
29477222 DOI: 10.1016/bs.afnr.2017.12.006,
https://pubmed.ncbi.nlm.nih.gov/29477222/
44) Yen-Ming Chan, Regan Bailey, Deborah L O'Connor, Folate, PMID: 23319130 PMCID:
PMC3648733 DOI: 10.3945/an.112.003392,
https://pubmed.ncbi.nlm.nih.gov/23319130/
45) Ralph Green, Ananya Datta Mitra, Megaloblastic Anemias: Nutritional and Other
Causes, PMID: 28189172 DOI: 10.1016/j.mcna.2016.09.013,
https://pubmed.ncbi.nlm.nih.gov/28189172/
46) Doreen Gille, Alexandra Schmid, Vitamin B12 in meat and dairy products, PMID:
26024497 DOI: 10.1093/nutrit/nuu011, https://pubmed.ncbi.nlm.nih.gov/26024497/
47) Jens Lykkesfeldt, Alexander J Michels, Balz Frei, Vitamin C, PMID: 24425716 PMCID:
PMC3884093 DOI: 10.3945/an.113.005157
https://pubmed.ncbi.nlm.nih.gov/24425716/
160
48) Anitra C Carr, Silvia Maggini, Vitamin C and Immune Function, PMID: 29099763
PMCID: PMC5707683 DOI: 10.3390/nu9111211,
https://pubmed.ncbi.nlm.nih.gov/29099763/
49) Jane Higdon, Victoria J. Drake, Barbara Delage, Connie M. Weaver, Linus Pauling
Institute, Calcium, Micronutrient Information Center,
https://lpi.oregonstate.edu/mic/minerals/calcium
50) R P Heaney, Calcium, dairy products and osteoporosis, PMID: 10759135 DOI:
10.1080/07315724.2000.10718088 https://pubmed.ncbi.nlm.nih.gov/10759135/
51) Ulrike Trautvetter, Bianka Ditscheid, Gerhard Jahreis, Michael Glei, Habitual Intakes,
Food Sources and Excretions of Phosphorus and Calcium in Three German Study
Collectives, PMID: 29393923 PMCID: PMC5852747 DOI: 10.3390/nu10020171,
https://pubmed.ncbi.nlm.nih.gov/29393923/
52) John A. Raven, RNA function and phosphorus use by photosynthetic organisms,
PMCID: PMC3872737, PMID: 24421782, Published online 2013 Dec 26. doi:
10.3389/fpls.2013.00536, https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3872737/
54) Ryu Yamanaka, Sho Tabata, Yutaka Shindo, Kohji Hotta, Koji Suzuki, Tomoyoshi
Soga, Kotaro Okaa, Mitochondrial Mg2+ homeostasis decides cellular energy
metabolism and vulnerability to stress, PMCID: PMC4960558, PMID: 27458051,
Published online 2016 Jul 26. doi: 10.1038/srep30027,
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4960558/
55) W B Vernon, The role of magnesium in nucleic-acid and protein metabolism, PMID:
2472534, h
ttps://pubmed.ncbi.nlm.nih.gov/2472534/
57) Carmen Andrea Pfortmueller, Dominik Uehlinger, Stephan von Haehling,3 and Joerg
Christian Schefold, Serum chloride levels in critical illness—the hidden story, PMCID:
PMC5899079, PMID: 29654387,
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5899079/
58) Jennifer S Hanberg , Veena Rao, Jozine M Ter Maaten , Olga Laur, Meredith A Brisco,
F Perry Wilson, Justin L Grodin , Mahlet Assefa , J Samuel Broughton, Noah J
161
Planavsky , Tariq Ahmad, Lavanya Bellumkonda , W H Wilson Tang, Chirag R Parikh ,
Jeffrey M Testani, Hypochloremia and Diuretic Resistance in Heart Failure:
Mechanistic Insights , PMID: 27507113 PMCID: PMC4988527 DOI:
10.1161/CIRCHEARTFAILURE.116.003180,
https://pubmed.ncbi.nlm.nih.gov/27507113/
60) Minghao Feng, Bingqing Tang, Steven H. Liang,b, Xuefeng Jianga, Sulfur Containing
Scaffolds in Drugs: Synthesis and Application in Medicinal Chemistry, PMCID:
PMC4877035, PMID:
26369815,https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4877035/
61) Joanne F Doleman, Katrijn Grisar, Lena Van Liedekerke, Shikha Saha, Mark Roe,
Henri S Tapp, Richard F Mithen, The contribution of alliaceous and cruciferous
vegetables to dietary sulphur intake , PMID: 28551250 PMCID: PMC5460521 DOI:
10.1016/j.foodchem.2017.04.098, h ttps://pubmed.ncbi.nlm.nih.gov/28551250/
62) Laura Percy, Diana Mansour, Ian Fraser, Iron deficiency and iron deficiency anaemia
in women, PMID: 28029503 DOI: 10.1016/j.bpobgyn.2016.09.007,
https://pubmed.ncbi.nlm.nih.gov/28029503/
63) S Grantham-McGregor, C Ani, A review of studies on the effect of iron deficiency on
cognitive development in children, PMID: 11160596 DOI: 10.1093/jn/131.2.649S,
https://pubmed.ncbi.nlm.nih.gov/11160596/
64) Ananda S Prasad, Zinc in Human Health: Effect of Zinc on Immune Cells, PMCID:
PMC2277319, PMID: 18385818, Published online 2008 Apr 3. doi:
10.2119/2008-00033.Prasad,
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2277319/
65) Parosh Kadir Muhamed, Steen Vadstrup, Zinc is the most important trace element,
PMID: 25096007, h
ttps://pubmed.ncbi.nlm.nih.gov/25096007/
67) Longman Li, Xiaobo Yang, The Essential Element Manganese, Oxidative Stress, and
Metabolic Diseases: Links and Interactions,
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5907490/
68) Michael Aschner, Keith Erikson, Manganese, PMID: 28507016 PMCID: PMC5421128
DOI: 10.3945/an.117.015305, h ttps://pubmed.ncbi.nlm.nih.gov/28507016/
162
69) Ujang Tinggi, Selenium: its role as antioxidant in human health, PMCID:
PMC2698273, PMID: 19568888, Published online 2008 Feb 28. doi:
10.1007/s12199-007-0019-4,
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2698273/
70) Mara Ventura, Miguel Melo, Francisco Carrilho, Selenium and Thyroid Disease: From
Pathophysiology to Treatment,
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5307254/
72) A scientific review: the role of chromium in insulin resistance, PMID: 15208835,
https://pubmed.ncbi.nlm.nih.gov/15208835/
75) Russ Hille, Takeshi Nishino, Florian Bittner, Molybdenum enzymes in higher
organisms, PMCID: PMC3079273, PMID: 21516203, doi: 10.1016/j.ccr.2010.11.034,
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3079273/
76) Katarzyna Czarnek, Sylwia Terpiłowska, Andrzej K. Siwicki, Selected aspects of the
action of cobalt ions in the human body, PMCID: PMC4637398, PMID: 26557039,
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4637398/
78) Marc P. McRae, J Chiropr Med, Dietary Fiber Is Beneficial for the Prevention of
Cardiovascular Disease: An Umbrella Review of Meta-analyses, PMCID:
PMC5731843, PMID: 29276461,
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5731843/
79) Maryam S. Farvid, A. Heather Eliassen, Eunyoung Cho, Xiaomei Liao, Wendy Y. Chen
and Walter C. Willett, Dietary Fiber Intake in Young Adults and Breast Cancer Risk,
Pediatrics February 2016, peds.2015-1226; DOI:
https://doi.org/10.1542/peds.2015-1226,
https://pediatrics.aappublications.org/content/early/2016/01/28/peds.2015-1226
163
81) Teresa T Fung, Frank B Hu, Mark A Pereira, Simin Liu, Meir J Stampfer, Graham A
Colditz, Walter C Willett, Whole-grain intake and the risk of type 2 diabetes: a
prospective study in men, h ttps://pubmed.ncbi.nlm.nih.gov/12197996/
82) Matthias B Schulze, Simin Liu, Eric B Rimm, JoAnn E Manson, Walter C Willett, Frank
B Hu, Glycemic index, glycemic load, and dietary fiber intake and incidence of type 2
diabetes in younger and middle-aged women, PMID: 15277155 DOI:
10.1093/ajcn/80.2.348, h
ttps://pubmed.ncbi.nlm.nih.gov/15277155/
83) G Vighi, F Marcucci, L Sensi, G Di Cara, F Frati, Allergy and the gastrointestinal
system, PMCID: PMC2515351, PMID: 18721321,
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2515351/
84) Jessica Stoller-Conrad, Microbes Help Produce Serotonin in Gut, April 09, 2015,
https://www.caltech.edu/about/news/microbes-help-produce-serotonin-gut-46495
86) Your Gut Can Influence How You Feel: It All Starts with GABA and Serotonin,
https://bodyecology.com/articles/your-gut-can-influence-how-you-feel-it-all-starts-wi
th-serotonin/
87) Núria Macha, Dolors Fuster-Botellaa, Endurance exercise and gut microbiota: A
review, PMCID: PMC6188999, PMID: 30356594,
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6188999/
88) Jodi D Stookey, Florence Constant, Barry M Popkin, Christopher D Gardner, Drinking
water is associated with weight loss in overweight dieting women independent of
diet and activity, PMID: 18787524 DOI: 10.1038/oby.2008.409,
https://pubmed.ncbi.nlm.nih.gov/18787524/
89) Michael Boschmann, Jochen Steiniger, Uta Hille, Jens Tank, Frauke Adams, Arya M
Sharma, Susanne Klaus, Friedrich C Luft, Jens Jordan, Water-induced thermogenesis,
PMID: 14671205 DOI: 10.1210/jc.2003-030780,
https://pubmed.ncbi.nlm.nih.gov/14671205/
164
90) Vinu A Vij, Anjali S Joshi, Effect of 'water induced thermogenesis' on body weight,
body mass index and body composition of overweight subjects, PMID: 24179891
PMCID: PMC3809630 DOI:10.7860/JCDR/2013/5862.3344,
https://pubmed.ncbi.nlm.nih.gov/24179891/
91) Vinu Ashok Kumar Vij, Anjali S Joshi, Effect of excessive water intake on body weight,
body mass index, body fat, and appetite of overweight female participants, PMID:
25097411 PMCID: PMC4121911 DOI: 10.4103/0976-9668.136180,
https://pubmed.ncbi.nlm.nih.gov/25097411/
94) Ronald M. Krauss, Robert H. Eckel, Barbara Howard, Lawrence J. Appel, Stephen R.
Daniels, Richard J. Deckelbaum, John W. ErdmanJr, Penny Kris-Etherton, Ira J.
Goldberg, Theodore A. Kotchen, Alice H. Lichtenstein, William E. Mitch, Rebecca
Mullis, Killian Robinson, Judith Wylie-Rosett, Sachiko St. Jeor, John Suttie, Diane L.
Tribble, and Terry L. Bazzarre, Revision 2000: A Statement for Healthcare
Professionals From the Nutrition Committee of the American Heart Association,
Originally published31 Oct 2000https://doi.org/10.1161/01.CIR.102.18.2284,
https://www.ahajournals.org/doi/full/10.1161/01.cir.102.18.2284
95) Christopher N Blesso, Catherine J Andersen, Jacqueline Barona, Jeff S Volek, Maria
Luz Fernandez, Whole egg consumption improves lipoprotein profiles and insulin
sensitivity to a greater extent than yolk-free egg substitute in individuals with
metabolic syndrome, PMID: 23021013 DOI: 10.1016/j.metabol.2012.08.014,
https://pubmed.ncbi.nlm.nih.gov/23021013/
97) Gisella Mutungi, Joseph Ratliff, Michael Puglisi, Moises Torres-Gonzalez, Ushma
Vaishnav, Jose O Leite, Erin Quann, Jeff S Volek, Maria Luz Fernandez, Dietary
cholesterol from eggs increases plasma HDL cholesterol in overweight men
consuming a carbohydrate-restricted diet, PMID: 18203890 DOI:
10.1093/jn/138.2.272, https://pubmed.ncbi.nlm.nih.gov/18203890/
165
100) Steven H. Zeisel, Kerry-Ann da Costa, Choline: An Essential Nutrient for Public
Health, PMCID: PMC2782876, PMID: 19906248,
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2782876/
101) Silvio Buscemi, Davide Corleo, Francesco Di Pace, Maria Letizia Petroni, Angela
Satriano, Giulio Marchesini, The Effect of Lutein on Eye and Extra-Eye Health, PMCID:
PMC6164534, PMID: 30231532,
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6164534/
103) F Bellisle, R McDevitt, A M Prentice, Meal frequency and energy balance, PMID:
9155494 DOI: 10.1079/bjn19970104, h ttps://pubmed.ncbi.nlm.nih.gov/9155494/
104) Jameason D Cameron , Marie-Josée Cyr, Eric Doucet, Increased meal frequency
does not promote greater weight loss in subjects who were prescribed an 8-week
equi-energetic energy-restricted diet, PMID: 19943985 DOI:
10.1017/S0007114509992984, https://pubmed.ncbi.nlm.nih.gov/19943985/
106) Aseem Malhotra, Saturated fat is not the major issue, doi:
https://doi.org/10.1136/bmj.f6340, https://www.bmj.com/content/347/bmj.f6340.full
108) Roberto Pecoits-Filho, Dietary protein intake and kidney disease in Western diet,
PMID: 17369718 DOI: 10.1159/000101003,
https://pubmed.ncbi.nlm.nih.gov/17369718/
166
109) M Blum, M Averbuch, Y Wolman, A Aviram, Protein intake and kidney function in
humans: its effect on 'normal aging', PMID: 2912408,
https://pubmed.ncbi.nlm.nih.gov/2912408/
110) Carol S Johnston 1 , Carol S Day, Pamela D Swan, Postprandial thermogenesis is
increased 100% on a high-protein, low-fat diet versus a high-carbohydrate, low-fat
diet in healthy, young women , PMID: 11838888 DOI:
10.1080/07315724.2002.10719194, https://pubmed.ncbi.nlm.nih.gov/11838888/
111) Enrica Golia, Giuseppe Limongelli, Francesco Natale, Fabio Fimiani, Valeria
Maddaloni, Ivana Pariggiano, Renatomaria Bianchi, Mario Crisci, Ludovica D'Acierno,
Roberto Giordano, Gaetano Di Palma, Marianna Conte, Paolo Golino, Maria
Giovanna Russo, Raffaele Calabrò, Paolo Calabrò, Inflammation and cardiovascular
disease: from pathogenesis to therapeutic target, PMID: 25037581 DOI:
10.1007/s11883-014-0435-z, https://pubmed.ncbi.nlm.nih.gov/25037581/
112) K N Ansari, The free radicals--the hidden culprits--an update, PMID: 9567509,
https://pubmed.ncbi.nlm.nih.gov/9567509/
113) Dagfinn Aune, Teresa Norat, Pål Romundstad, Lars J Vatten, Whole grain and
refined grain consumption and the risk of type 2 diabetes: a systematic review and
dose-response meta-analysis of cohort studies, PMID: 24158434 DOI:
10.1007/s10654-013-9852-5, https://pubmed.ncbi.nlm.nih.gov/24158434/
114) Dagfinn Aune, Teresa Norat, Pål Romundstad, Lars J Vatten, Whole grain and
refined grain consumption and the risk of type 2 diabetes: a systematic review and
dose-response meta-analysis of cohort studies, PMID: 24158434 DOI:
10.1007/s10654-013-9852-5, https://pubmed.ncbi.nlm.nih.gov/24158434/
115) Should you get your nutrients from food or from supplements?, Harvard Health
Letter, Published: May, 2015,
https://www.health.harvard.edu/staying-healthy/should-you-get-your-nutrients-fro
m-food-or-from-supplements
167