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AEROSOLS AND AIRBORNE INFECTION CONTROL IN THE

DENTAL OFFICE
An overview on the importance of size of aerosolized particles and nanotechnology
Author: S Bordin-Aykroyd
as an essential foundation in the control of airborne disease. admin@aaofh.org
Abstract Control ID#: 3577546

TABLE 1. CLASSIFICATION OF BIOAEROSOL PARTICLES

BACKGROUND The size of particles is a Classification of particles Particle size Sites found
according to size type
determinant factor in aerosol behavior and plays an coarse 2.5 10 µm spatter > 50 floor, countertop, sink, bracket,
essential role in the spread and mitigation of fine < 2.5 µm µm table, computer, patient or operator
airborne diseases. They are influenced by ultrafine < 0.1 µm droplet Air suspension until evaporation
Corona Virus SARS-CoV-2 0.06 to 0.14 µm or µm
environmental conditions such as temperature, droplet 5 µm Contains microorganisms. Can
~120 nm
humidity, and airflow distribution12. Due to their size, Crown spikes of Corona Virus 9 - 12 nm nuclei contaminate surfaces within 3 feet
SARS-CoV-2 and remain airborne for hours
composition, ability to remain suspended and travel
long distances, small, infected aerosolized particles The risk of cross-infection by SARS-CoV-2 in the dental office is confirmed by research showing positive salivary tests,
pose a considerable health risk12, especially in the indicating the possibility of transmission through the spread of saliva as respiratory viruses usually spread via direct
dental operatory. contact or splatter and aerosol production from mouth and nose22. More than 700 microbial species have been identified in
saliva. Studies suggest that the transmission of virus can be closely connected with saliva and salivary glands may
AIM To provide an overview on the importance of
become a potential source cross-infection by the SARS-CoV-2 virus23.
particle size and nanotechnology as an essential
Some viral aerosolized particles, can potentially travel up to 20 feet18 and aerosolized droplet nuclei, containing significant
foundation in the control of airborne disease.
amounts of viruses can remain suspended in the air and capable of contamination for many days 12,15,2.

METHOD Literature review 2010 - 2020


Airborne transmission has often been attributed to infectious droplet nuclei produced by the desiccation of suspended
Bioaerosol
Type of procedure Size of particle
classification 3, where there is a predominance of pathogens. Those can be produced by coughing and
Ultrasonic aerosol particles 0.001 to 100 µm aerosol + splatter
sneezing and be immediately respirable3. Most particles in exhaled breath are smaller than 4 m, with an average size
90 % of general dental
procedures
< 5 µm aerosols between 0.7 and m 49.
95% laser plume water water
bioaerosols, Small particle aerosols are associated with most respiratory infections3.
including cellular
Infected
5 % laser plume debris, blood
droplets Entry size Body part
fragments, and The capacity of particles to be deposited in different parts of
bacteria. capacity
the body is also determined by their sizes11,12,13,17,18. Nose 6 12 Upper respiratory system
INTRODUCTION - Devices utilized in dental filtering
operatories, such as high-speed handpieces, Nasal filtration of particles smaller than 0.5 microns is low, so 0.25 0.1 µm Alveoli / lungs
ultrasonic devices, and some types of lasers, can smaller particles can pass easily into the lower respiratory tract < 0.1 µm Bloodstream, organs, heart &
brain
generate aerosol, splatter, or droplets, which to infect the lungs.

contains small contaminant particles associated with This can be of clinical significance, since the
Fine particles (< 2.5 microns), capable of infecting the lower
the devices, with ultrasonics and high- speed average size of the corona virus is 120 nm1 and it
respiratory system, contain 8.8 times more virus than coarse
handpieces producing more airborne contamination can be present in very small droplets that may
particles and can be found on exhaled breath4.
than any other dental device. Ultrasonic not be picket-up by HEPA filters that operate on
instrumentation can transmit 100,000 microbes per Particles smaller than 0.1 microns can access and travel the a micron scale
cubic ft. 3,19,20,21. bloodstream and directly infect organs, heart and brain11,12,13.

One of the most effective methods of reducing aerosols in dentistry are high-volume evacuators (HVE), however, recently, ASHRAE recommends the inclusion of extra room
equipment to air conditioning filters and HVEs, such as stand-alone air-purifiers, UV light devices, etc., requiring additional technological solutions for this and future pandemics.

Advanced Oxidation Processes (AOPs) Photocatalytic disinfection Use nano-particles such as Titanium dioxide (TiO2) or zinc oxide (ZnO) as photosensitizing agents
and ultra-violet (UV) radiation as the activator of excitation24.
RESULTS
In this review, we explored the implication of particle size in airborne infection viability and the importance of a good filtering system. We also found a 10 years review of supportive
evidence of the efficacy of up to 99.99% of -Cov family.
DISCUSSION REFERENCES
://www.cdc.gov/coronavirus/2019-ncov/science/science-briefs/sars-cov-2-transmission.html

Given the fundamental mechanism particle size plays on airborne disease it should receive more emphasis 2. https://www.ashrae.org/technical-resources/resources

3. Bake B, Larsson P, Ljungkvist G, Ljungstrom E, Olin AC. Exhaled particles and small airways. Respir Res 2019; 20: 8.

whilst investigating methods of mitigation of viral respiratory diseases. 4.. Bourouiba, Lydia. Turbulent gas clouds and respiratory pathogen emissions: potential implications for reducing transmission of COVID-19. Journal of the American Medical Association

5. Brown JH, Cook KM, Ney FG, Hatch T. Influence of particle size upon the retention of particulate matter in the human lung. Am J Public Health Nations Health 1950; 40: 450 80.
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6. Harper GJ, Morton JD. The respiratory retention of bacterial aerosols: experiments with radioactive spores. J Hyg 1953; 51: 372 85.

7. Jan-Alexander Schwab MD, Matthias Zenkel MD. Filtration of Particulates in the Human Nose. The laryngoscope. 04 January 2009.

If particle size plays such an important role in the ability of the droplets to remain suspended in the air for 8. Kevin P Fennelly. Particle sizes of infectious aerosols: implications for infection control. Lancet Respir Med 2020; 8: 914 24

9. LidiaMorawska et al. How can airborne transmission of COVID-19 indoors be minimised? Environmental International. Volume 142, September 2020, 105832.

many hours and the highly transmissible SARS-CoV-2 are so small that it can colonize each particle in great 10. Miller, Robert L. Characteristics of blood-containing aerosols generated by common powered dental instruments. American Industrial Hygiene Association Journal, v. 56, n. 7, p. 670-676, 1995. PMID: 7618605. DOI: 10.1080/15428119591016683.

11. Milton DK, Fabian MP, Cowling BJ, Grantham ML, McDevitt JJ. Influenza virus aerosols in human exhaled breath : particle size, culturability, and effect of surgical masks. PLoS Pathog 2013; 9: e1003205.

numbers, one could assume that this may directly influence viral load and requires further investigation. 12. Siegel JD, Rhinehart E, Jackson M, Chiarello L, Health Care Infection. Control Practices Advisory Committee. 2007 Guideline for isolation precautions: preventing transmission of infectious agents in health care settings. Am J Infect Control 2007; 35: S65 164.

13. Van-Doremalen N, Bushmaker T, Morris DH, et al. Aerosol and surface stability of SARS-CoV-2 as compared with SARS-CoV-1. New England Journal of Medicine. 2020. DOI: 10.1056/NEJMc2004973.

14. Verbeek JH, Rajamaki B, Ijaz S, et al. Personal protective equipment for preventing highly infectious diseases due to exposure to contaminated body fluids in healthcare staff. Cochrane Database Syst Rev 2020; 5: CD011621.

Also, if SARS-cov2 can be present in great numbers in particles smaller than < 0.1 µm and those particles can
15. Walls A., Tortorici M.A., Bosch B.J., Frenz B., Rottier P.J., DiMaio F. Crucial steps in the structure determination of a coronavirus spike glycoprotein using cryo electron microscopy. Protein Sci. 2017; 26:113 121.

- 1 618.

directly access the bloodstream, organs and brain, could there possibly be a relationship between the inhaled
17. WHO. Critical preparedness, readiness and response actions for COVID-19: interim guidance, 19 March 2020. Available at: <https://apps.who.int/iris/bitstream/handle/10665/331494/WHO-2019-nCoV-Community_Actions-2020.2-eng.pdf>. Accessed in: May 2020.

18. Zemouri, Charifa et al. A scoping review on bio-aerosols in healthcare and the dental environment. PloS one, May22, v. 12, n. 5, p. 8-25, 2017. doi.org/10.1371/journal.pone.0178007.

particle sizes, with its respective viral load, and COVID-19 symptoms severity and/or post-covid sequalae?
19. Mechanisms of Laser-Tissue Interaction: II. Tissue Thermal Properties Ansari,et al, J Lasers Med
Sci. 2013 Summer; 4(3): 99 106
20. Laser plumes Health care facilities. http://www.ccohs.ca/oshanswers/phys_agents/laser_plume.html. Accessed 8/11/2012.
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23. LI Y, Ren B, Peng X, Hu T, Li J, Gong T, Tang B, Xu X, Zhou X. Saliva is a non-negligible factor in the spread of COVID-19. Mol Oral Microbiol. 2020 May 4.
24. Bogdanetal. Nanoscale Research Letters (2015) 10:309 DOI 10.1186/s11671-015-1023-z

CONCLUSION
In addition to managing environmental factors such as air dynamics, temperature and humidity that influence the size and transport mechanism of particles; using dental tools that
produce less contaminant aerosols and advanced airflow technology, this review indicates that nanotechnology photocatalytic filters may offer an effective additional solution to the
control of aerosols and transmission of airborne disease in the dental office.

Disclaimer: The author received no financial support and declares no conflict of interest

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