Analysis of Pierce Amino Acid Standard H using Vanquish Flex

10082015A #31 Amino acids H CAD_1

16.0
pA HPLC Thermo Scientific™ Acclaim™ PA2
PA2,
Amino Acids column: 2.2 µm, 2.1 x 250 mm
15.0 1 Alanine
2 Eluent A: 0.15% NFPA in Water,
2 Arginine
14.0 3 Aspartic Acid Eluent B: 0.2% TFA in Acetonitrile
4 Cystine Eluent C: 5% Acetic Acid
13 0
13.0 5 Gl t i A
Glutamic Acid
id C l
Column 40 °C
6 Glycine Temp.:
12.0 7 Histidine 10 12
Detector: VCAD, 20 Hz, 5 s, 1.00 PFV
8 IsoLeucine
3/14 9 50 °C evaporation temp.
11.0 9 Leucine 7 8
10 y
Lysine Flow 0.5 mL/min
10.0 11 Methionine Rate:
12 Phenylalanine 16
Gradient:
9.0 13 Proline
15 14 Serine
13
8.0 5 15 Threonine
pA]

1 16 Tyrosine 17 11
Current [p

7.0 17 Valine
6

6.0
4
5.0

4.0

3.0

2.0

1.0

0.0

-1.0

-2.0
min
0.00 1.25 2.50 3.75 5.00 6.25 7.50 8.75 10.00 11.25 12.50 13.75 15.00 16.25 18.00
Time [min]