Name: DAYAGAN, GWYNETH MARIE M.

Date: July 1, 2021

EXERCISE 2

ACCESSIONING & CUTTING OF SPECIMEN FOR TISSUE

PROCESSING
Specimens submitted to the histopathology laboratory must be entered into the
surgical pathology database via accessioning process. The receptionist records or numbers the
tissue specimen. Tissues are classified based on the type of specimen received, for the purpose
of easy identification, ease in handling and fast retrieving of results in case future reports are
needed. Tissue specimens are prepared by the medical technologists for the pathologists to
describe and cut.

Unlabeled specimens are absolutely unacceptable for accessioning. Proper

identification includes matching the data on the specimen container label with the
accompanying histopathology request form. If there are inconsistencies between the two, the
specimen and request form are returned to the operating room for correction by the staff
concerned. Specimen received in the laboratory, whether tissue or smear, are numbered upon
receipt for eases in handling and easy retrieval when further reports or investigations are needed.

1. OBJECTIVES
At the end of the laboratory exercise, the students should be able to:

1.1 Demonstrate the proper numbering of specimens immediately upon receipt.

1.2 Demonstrate the proper cutting and appropriate size of tissue needed for the

process.

2. MATERIALS

2.1 Tissue specimen: Liver & Lung 2.5 Pencil

2.2 Sharp knife / Dissecting knife / Scalpel blade 2.6 Ruler

2.3 Dental Floss 2.7 Filter paper

2.4 Scissors
3. PROCEDURE

3.1 Cut ½ x 2 inch of filter paper.

3.2 Using a pencil, write the number corresponding assigned to you by the instructor.

3.3 Cut a 2.5 x 2 x 0.5 cm tissue

3.4 Tie the tissue using one end of a dental floss. On the other end

4. RESULT/OBSERVATION

HISTOPATHOLOGIC REPORT SAMPLE:

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4.1 Draw and label tissue specimen based in the histopathologic report inside a sample container
with fixative and proper label attached to the container.

4.2 Copy the histopathologic report attached in this activity sheet.

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5. STUDY QUESTIONS

5.1 Why is pencil used to label the filter paper for tissue processing?

Pencils are used to label the filter paper because it creates a permanent and an
unaffected mark after coated with wax. When using a ballpen or any ink-based marker,
there is a tendency that it will get smudged/ smeared when dipped into the wax because
they are not solvent resistant.

5.2 Why are the specimen cut before tissue processing?

Cutting the specimen into its ideal size before tissue processing allows the fixative
or solution to be absorbed properly. During specimen examination, tissues should be cut
thinly in order to permit enough light to pass through the microscope. In addition, cutting
the specimens aids the pathologist in choosing the parts of the specimen to be observed.

5.3 What are the basic information that should be recorded upon the receipt of tissue
specimen?

• Patient's name
• Patient's age and sex
• Patient’s date of birth
• Patient's location (hospital number, ward number)
• Physician’s Name
• Pathology Number
• Collection date and time
• Specimen type and/or source
• Test required (note special handling requirements if applicable)

3.2 Enumerate the 5 criteria for rejection of gross specimen.

1. Incomplete or inconsistent documentation between requisition form and

specimen labels
2. Incorrectly labelled/mislabeled or unlabeled specimen and/or specimen
container
3. Leaking specimen container
4. Absent/missing clinical data or history
5. Insufficient, incomplete, or unsuitable specimen for procedure

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3.3 Enumerate 4 pre-analytical factors that impact tissue diagnosis, prognostication and
theragnostic

1. Tissue Degradation
a. Warm Ischemia
b. Cold Ischemia
2. Fixation of tissues
3. Properly labelled/ filled-up surgical pathologic requisition form
4. Proper accessioning procedure

Student’s Name : DAYAGAN, GWYNETH MARIE M. Group& Sec: GROUP 2 – C Date: 7/1/2021
Exercise No. 2: ACCESSIONING & CUTTING OF SPECIMEN FOR TISSUE PROCESSING

CRITERIA BEGINNING DEVELOPING ACCOMPLISHED EXEMPLARY SCORE

(5) (10) (15) (20)
Ability to Answer Able to answer Able to answer Able to answer Able to answer
Questions 25% of the 50% of the 75% of the item 100% of the
Able to answer all items required. items required. required. item required.
the required items
in the activity .
Content/Knowledge Presents 25% Presents 50% Presents 75% Presents 100%
Presented of the of the of the of the
Presents knowledge knowledge knowledge knowledge
scientific/practical required. required. required. required.
facts based on
knowledge of the
subject.
Finding Relevant 25% of the 50% of the 75% of the 100% of the
Information answers were answers were answers were answers were
Presents facts and supported by supported by supported by supported by
relevant facts & relevant facts & relevant facts & relevant facts & relevant
information to information. information. information. information.
support the
answers.
Sentence Fluency & 25% of the 50% of the 75% of the 100% of the
Organization of sentences were sentences were sentences were sentences were
Thoughts clear & clear & clear & clear &
Sentences are understandable. understandable. understandable. understandable.
very clear & Lacks Few ideas Most of the All ideas
understandable. spontaneity of showed ideas showed showed
ideas. spontaneity. spontaneity. spontaneity.
Ideas were
presented in order
and spontaneous.
Punctuality Did not able to Submitted on Submitted on Submitted on or
Submitted the submit on the the closing date the closing and before the due
output on the due date & but exceed 1 a day after the and closing
closing date. due date. dates.
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 specified date & day on the due
time. date.
TOTAL SCORE

Preceptor’s Signature: ____________________________