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  • QC Herbal, Metabolomik, Minggu Ke-10

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    - The document discusses herbal drug technology and metabolomics, which is the comprehensive study of small molecule metabolites in biological systems. - Metabolomics uses techniques like NMR, MS, GC to separate and characterize metabolites, which can be used to identify differences between biological samples. - Applications of metabolomics include toxicology testing, clinical trials, food and beverage testing, and more. It provides a global profile of metabolic changes in response to stimuli. - A case study on extracting antioxidative anthraquinones from Morinda citrifolia roots using microwave assisted extraction is presented to demonstrate metabolomics workflow and analysis.

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    - The document discusses herbal drug technology and metabolomics, which is the comprehensive study of small molecule metabolites in biological systems. - Metabolomics uses techniques like NMR, MS, GC to separate and characterize metabolites, which can be used to identify differences between biological samples. - Applications of metabolomics include toxicology testing, clinical trials, food and beverage testing, and more. It provides a global profile of metabolic changes in response to stimuli. - A case study on extracting antioxidative anthraquinones from Morinda citrifolia roots using microwave assisted extraction is presented to demonstrate metabolomics workflow and analysis.

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    11 views18 pages

    QC Herbal, Metabolomik, Minggu Ke-10

    Uploaded by

    Rifqi Al Afuw
    - The document discusses herbal drug technology and metabolomics, which is the comprehensive study of small molecule metabolites in biological systems. - Metabolomics uses techniques like NMR, MS, GC to separate and characterize metabolites, which can be used to identify differences between biological samples. - Applications of metabolomics include toxicology testing, clinical trials, food and beverage testing, and more. It provides a global profile of metabolic changes in response to stimuli. - A case study on extracting antioxidative anthraquinones from Morinda citrifolia roots using microwave assisted extraction is presented to demonstrate metabolomics workflow and analysis.

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    of 18

    HERBAL DRUG TECHNOLOGY

    PLANT
    METABOLOMIC

    Dr. Oeke Yunita, S.Si., M.Si., Apt.


    Pharmaceutical Biology,
    Faculty of Pharmacy, University of Surabaya
    INDONESIA
    oeke@staff.ubaya.ac.id.
    QUALITY ASSURANCE
    PRODUK HERBAL

    IDENTIFIKASI LANGSUNG
    • Organoleptis: bau, rasa, warna dll
    • Morfologi: bentuk tepi daun, jenis buah dll

    IDENTIFIKASI MIKROSKOPIS

    IDENTIFIKASI KANDUNGAN KIMIA

    IDENTIFIKASI MOLEKULER (DNA)

    2
    Summer Program, Mahidol - UBAYA, 2014 3
    ‘omics’ technology
    GENOMICS TRANSCRIPTOMICS PROTEOMICS METABOLOMICS

    Target Gene, mRNA Protein Low molecular


    material chromosome (genetic code) (function of the weight
    (genetic code) protein) metabolites

    MW 100,000- 100,000-120,000 5,000-20,000 100- 5000


    120,000
    Analysis Mapping, Sequencing Separation, Separation,
    sequencing characterization characterization

    Methods DNA Hybridization 2D gel, Maldi NMR, MS, GC,


    sequencer, TOF LC
    PCR
    Human 30,000 >109? ~2,500
    genome

    4
    • METABOLOMICS
    – Newly emerging field of 'omics' research
    – Comprehensive and simultaneous systematic determination
    of metabolite levels in the metabolome and their changes
    over time as a consequence of stimuli

    • Metabolome
    – Refers to the complete set of small-molecule metabolites
    – Dynamic

    • Metabolites
    – Intermediates and products of metabolism (MW < 1500 Da)
    – Examples include antibiotics, pigments, carbohydrates, fatty
    acids and amino acids
    – Primary and secondary metabolites 5
    Summer Program, Mahidol - UBAYA, 2014
    General flow for metabolomics

    1. Is there a difference between samples?

    2. What is the difference between samples?

    3. What is the reason of difference?

    Summer Program, Mahidol - UBAYA, 2014 6


    METABOLOMICS APPLICATIONS
    • Toxicology Testing • Genetic Disease Tests
    • Clinical Trial Testing • Nutritional and Herbal
    • Fermentation Monitoring Analysis
    • Food & Beverage Tests • Clinical Blood Analysis
    • Nutraceutical Analysis • Clinical Urinalysis
    • Drug Phenotyping • Cholesterol Testing
    • Water Quality Testing • Drug Compliance
    • Petrochemical Analysis • Transplant Monitoring
    • MRS and CS imaging

    Summer Program, Mahidol - UBAYA, 2014 7


    CASE STUDY

    MAE of antioxidative anthraquinones from roots of Morinda citrifolia, Hemwimon et


    al., Separation and Purification Technology, 2007, 54, 44-50
    Summer Program, Mahidol - UBAYA, 2014 8
    9
    Summer Program, Mahidol - UBAYA, 2014 10
    Scaling of the metabolite problem

    what is the objective – what is the goal.

    Summer Program, Mahidol - UBAYA, 2014 11


    Summer Program, Mahidol - UBAYA, 2014 12
    Summer Program, Mahidol - UBAYA, 2014 13
    14
    Summer Program, Mahidol - UBAYA, 2014
    15
    Summer Program, Mahidol - UBAYA, 2014
    METABOLOMICS WORKFLOW

    Biological or Tissue Samples Extraction Biofluids or Extracts

    ppm
    7 6 5 4 3 2 1

    Data Analysis Chemical Analysis 16


    Summer Program, Mahidol - UBAYA, 2014
    CHEMICAL PROFILES

    Data Preprocessing

    PRINCIPLE COMPONENT HIERARHICAL


    ANALYSIS (PCA) CLUSTER ANALYSIS
    17
    Summer Program, Mahidol - UBAYA, 2014
    Summer Program, Mahidol - UBAYA, 2014 18

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