Products and Capabilities: Single-Use Technology

Thermo Scientific
Single-Use Systems
Single-use technology
products and capabilities
Table of contents
Introduction
Introduction to Thermo Scientific BioProcess Containers............................................................................................................... 2

BioProcess Container Manufacturing Process.............................................................................................................................. 5
BioProcess Container Manufacuring Sites................................................................................................................................... 6
Quality Control and Assurance................................................................................................................................................ 7–9

Products and Services
CX5-14 Film............................................................................................................................................................................ 10
CX3-9 Film.............................................................................................................................................................................. 11
Aegis5-14 Film........................................................................................................................................................................ 12
Tubing Selection Guide....................................................................................................................................................... 13–14
Connection Selection Guide............................................................................................................................................... .15–16
Filters...................................................................................................................................................................................... 17
Fluid Transfer Assemblies......................................................................................................................................................... 18
2D BioProcess Container Systems...................................................................................................................................... 19–22
3D BioProcess Container Systems...................................................................................................................................... 23–25
Harvestainer Microcarrier Separation BPC and System......................................................................................................... 26–27
Tank Liners............................................................................................................................................................................. 28
Plastic Tanks, Drums and Accessories................................................................................................................................. 29–30
Large Volume Rigid Plastic Support Containers.......................................................................................................................... 31
Large Volume Stainless Steel Outer Containers.................................................................................................................... 32–33
Mixtainer BioProcess Container System.............................................................................................................................. 34–36
HyPerforma Single-Use Mixer DS 300................................................................................................................................. 37–42
HyPerforma Single-Use Mixer............................................................................................................................................. 43–49
Powdertainer BioProcess Container Systems....................................................................................................................... 50–51
HyPerforma Single-Use Bioreactor....................................................................................................................................... 52–56
HyPerforma Single-Use Fermentor...................................................................................................................................... 57–62
Temperature Control Units.................................................................................................................................................. 63–64
Bioreactor Control Solutions............................................................................................................................................... 65–68
Services and Support ........................................................................................................................................................69
Cell Line Development.......................................................................................................................................................70
Cell Culture Media.............................................................................................................................................................71–73
Cell Culture Feeds.............................................................................................................................................................74
FunctionMAX TiterEnhancer Functional Additive...................................................................................................................75
Analytics and Purification...................................................................................................................................................76–83
1
Introduction
cell culture
u Introduction to Thermo Scientific BioProcess Containers
For more than 20 years, Thermo Scientific has been pioneering single-use technology for
the biotech industry. We first launched single-use bioprocess containers (BPCs) in 1993
and launched our first single-use bioreactor (S.U.B.) in 2006; at a time when single-use
systems were regarded with some skepticism and doubt. Over the years, we helped drive
the advancement of single-use technologies by developing new single-use bioprocess
applications like the Thermo Scientific™ Harvestainer™ BPC for microcarrier bead
separation applications. In 2014, we were the first to launch a system specifically designed
for microbial fermentation–the Thermo Scientific™ HyPerforma™ Single-Use Fermentor
(S.U.F.).
Whether you are looking for an economical standard product solution or require a specific
custom-designed and -built system, we offer proven solutions at every scale. We offer a wealth of
experience, knowledge and solid quality processes behind every Thermo Scientific product, to help
you feel assured that your bioproduction process performs at its best.
As you browse the catalog, please keep in mind that you are looking at our standard product
catalog. We take pride in offering one of the largest standard component libraries in the industry,
which allows us to integrate connectors, tubing and sensors to design a BPC or an entire tubing
assembly to fit your specific needs.
Our experienced services organization is there to assist you with drawings, implementation, or
technical support to help ensure optimum production performance.
Our core technologies and expertise support consistent performing bioproduction and processes
that are optimized to help meet your needs. As part of the Thermo Scientific™ BioProduction
Portfolio, our Single-use systems have been tested and validated for easy integration with our
Gibco™ cell culture products.
Our products and services are specifically designed to enable proven performance through
innovative, efficient and highly effective upstream or downstream applications. From a single
source you can optimize production, improve process efficiency, add flexibility and fast track
product development and market introduction by selecting from our portfolio.
2 lifetechnologies.com
Introduction
What is a BPC?
The heart of our single-use solutions is our BioProcess Container (BPC). BPCs are single-
use flexible container systems commonly used for critical liquid handling applications in the
biopharmaceutical industry. BPC systems are cost-effective alternatives to conventional stainless
steel systems. They employ a novel design approach and are highly-valued for their cost-
effectiveness and utility. BPC components are readily integrated into a variety of high-performance
systems for all steps in the production of biologics.
Support operation Applications BPC solution

Media/buffer preparation Powder delivery Powdertainer™ BPC systems
Hydration in open top vessel Tank liners with outer support containers
Hydration in closed/sterile system Mixtainer™ BPC, Single-Use Mixer (S.U.M.)
Filtration Sterilization of media and buffers Custom BPC assembly or manifold including a filter
option
Bulk storage Sterile storage of media, Standard BPCs from 50 mL–2,000 L with outer support
buffers and intermediates containers
Custom BPCs to cover
additional options
Waste collection Non-aseptic collection of waste liquid from downstream Tank liners for non-aseptic applications
processing
Sampling Removal of sample volumes from bioreactors, mixers and Standard BPCs from 50 mL–50 L
storage containers for analysis
Custom manifolds
Custom tubing sets
Shipping Bulk sterile liquids, buffers, process liquids and Top and bottom drain BPCs up to 1,000 L with validated
intermediates between facilities shipping configurations
Bulk solutions or suspensions requiring mixing after shipping
Mixtainer BPC
3
Introduction
u Introduction to Thermo Scientific BioProcess Containers
The following table lists typical unit operations and corresponding applicable BPC solutions. Since
there is often a complex system such as a bioreactor or purification skid at the center of each unit
operation, complete single-use solutions are not common today. This has been changing with the
introduction of products such as the S.U.B. and S.U.F. Our development program seeks to expand
the list of single-use unit operations over the next few years.
Unit operation Application BPC solution

Bioreactor Cell culture in stirred-tank system Single-Use Bioreactor (S.U.B.)
Fermentor Fermentation in stirred-tank system Single-Use Fermentor (S.U.F.)
Harvest Collection and storage of harvest from a bioreactor Standard top and bottom drain BPCs from 50 L–1,500 L with outer
or fermentor support containers
Custom BPCs to cover transfer assemblies
Additional options
Separation Feeding and receiving liquid from the separation Standard top and bottom drain BPCs from 50 L–1,500 L with outer
system support containers
Custom BPCs to cover transfer assemblies
Additional options
Purification Feeding buffers to the Standard bottom drain BPCs from 50 L–1,500 L with outer support
purification system containers. Standard BPCs from 50 mL–50 L
Fraction collection and storage Custom BPCs to cover transfer assemblies, manifolds
Additional options
Filling Sterile bulk reservoir for filling systems Standard bottom drain BPCs from 50 L–1,500 L with outer support
containers
Transfer assemblies to transfer liquid from reservoir to filling system.
Custom BPCs to cover additional options
Introduction
Key features
• All chambers and BPCs are produced in state-of-the-art cGMP facilities

• 33,000 square feet of Class 10,000/ISO Class 7 production area
• Manufacturing roadmap to match manufacturing capacity to market demand
• Multiple facilities with common processes for manufacturing redundancy
• Manufacturing sites in USA and Europe
• Capabilities to produce chambers from 50 mL to 10,000 L
• Automated lines for producing BPC chambers
• Strong engineering support to design and maintain products and processes
BPC manufacturing process

Chamber manufacture—The main component of a Thermo Scientific™ BPC chamber is
plastic film produced by Thermo Scientific, or external suppliers in some cases. The other main
components are ports that enable tubing to be attached to the chamber. There are a number of
different port designs depending on the type of chamber.
The two main types of basic BPC chamber are:
Two-dimensional (2D)—This design is used for small, simple BPCs and is produced from two
sheets of film that are heat sealed around the perimeter to form a flat, or pillow-shaped, chamber.
The ports are heat sealed into the end seal or onto one of the faces of the chamber. Some designs
also have a handle formed by the sealing process or by insertion of a plastic bar at one end.
Three-dimensional (3D)—This design is used for larger and more complicated BPCs. A square
tube is formed by heat sealing sheets of film together. Ports are usually larger, and a greater range
of size and complexity of chamber designs is possible.
All chambers are produced in a Class 10,000/ISO Class 7 clean room in one of our
manufacturing facilities. A combination of automated and manual systems to help maximize
reliability, efficiency and flexibility is employed for all Thermo Scientific products.
Final assembly—Additional components are attached to the BPC chamber to produce a complete
BPC. This is done to either a standard or custom specification in a Class 10,000/ISO Class 7 clean
room in one of our manufacturing facilities. Assembly is a manual process to attain the configuration
flexibility required. Thermo Scientific™ fluid transfer assemblies are also produced to complement
BPC systems. Final assembly is done in the same areas to the same level of quality.
Packaging—BPCs are packaged and sealed in two independent outer layers while still in the Class
10,000/ISO Class 7 area. They are then placed in cardboard cartons labeled with product and lot
identification.
Sterilization—BPCs are sterilized in their outer packaging by a gamma irradiation dose of
25–40 kGy by external local contractors in the US and Europe.
Final inspection—Each lot of BPCs is 100% visually inspected against product
specifications.
5
Introduction
u Thermo Scientific BPC manufacturing sites
Assembly Center, Logan, Utah
This is our primary site for assembling BPCs, tubing sets and manifolds. In addition to
manufacturing, the Assembly Center site also houses quality control and assurance, materials
management, procurement, and production scheduling. This site’s 10,000 square feet of Class
10,000/ISO Class 7 clean room includes:
BPC final assembly—Standard BPCs based on both Thermo Scientific™ Aegis™ and CX Films
are assembled at our Assembly Center facility. Most custom BPC assemblies, tubing assemblies
and manifolds supplied to the Americas and Asia are assembled here.
Chamber manufacture—Our Assembly Center facility also has chamber production capabilities
for Aegis5-14, CX5-14, and CX3-9 Film-based BPC products, and in this way serves as the
primary back-up chamber production facility.
BioCenter, Logan, Utah
Our BioProcess Container Operations Center (BioCenter) is our primary BPC chamber
manufacturing facility. The BioCenter also houses our engineering laboratories, bioreactor
development center, quality control and assurance, project management, product management,
sales operations, finance, and human resources; as well as our applications, manufacturing,
and research and development engineering teams. The BioCenter has a total floor area of
61,000 square feet, with 14,000 square feet of Class 10,000/ISO Class 7 clean room area.
Our BioCenter includes:
Chamber manufacture—5 automated production lines manufacture the majority of our 2D

and 3D BPC chambers. The BioCenter’s manufacturing equipment provides great versatility in
batch size; film capability–CX3-9, CX 5-14, and Aegis5-14 Films; chamber size–from 50 mL to
3,000 L and larger; and chamber complexity–from simple tank liners, pillow-style 2D chambers
and square-tube style 3D chambers to our most complex S.U.B., S.U.F. and S.U.M.
Final assembly—Although primarily a chamber manufacturing facility, our BioCenter serves

as a back-up finished goods BPC assembly facility for BPC assemblies, tubing assemblies, and
manifolded assemblies.
Cramlington, UK
Our European BPC operations site is situated in Northern England. The current facility received
major upgrades and expansions in 2004, and again in 2011. These upgrades significantly
expanded our overall capability to serve the increasing demand for BPCs, fluid Transfer assemblies
and manifolds. The upgrades also provide enhanced chamber manufacturing redundancy.
The BPC manufacturing area currently consists of a 7,900 square feet of Class 10,000/ISO
Class 7 clean room and support areas. This facility houses:
Chamber manufacturing—We manufacture both Aegis and CX Film-based BPC chambers.
Final assembly—Both CX Film products and Aegis Film products can currently receive final
assembly at this site. Fluid transfer assemblies and manifolds are also assembled at this facility.
Introduction
u Quality control and assurance
To help ensure that BioProcess Container (BPC) Systems conform to the quality standards expected
in the bioprocess industry, they are subject to rigorous quality control in compliance with cGMP
(21 CFR Part 820) and ISO 9001:2000 from receipt of components to release of final product.
Component incoming inspection

Component type Inspection
Film Contamination
Gels or carbons
Width and gusset dimensions
Film thickness
Tensile strength and elongation–to ASTM D882
Chemical–using FT-IR analysis to ensure
consistency and reliability
Ports, fittings and tubing Appearance and visual inspection
Dimensions
Chambers Appearance
Seam and port seal strength
Dimensions
Leak and burst testing
Final BPC product inspection

Test Details
100% visual inspection on a light table Correctness
Completeness
Particulate debris
Defects and damage
Correct packaging
Hydro-burst test Performed according to a statisical process control
plan. The container is steadily inflated until there is
a breach of integrity.
Our production control processes help ensure complete lot traceability for each batch.
The production control document becomes the stepwise manufacturing record that physically
accompanies the lot through the manufacturing process. At the end of the process, the production
record is reviewed by Quality Assurance for completeness and correctness prior to release of the lot
and issuance of the certificate of analysis.
Lot record and Certificate of Analysis

Lot release Certificate of Analysis
Bill of materials Product name
Certificate of Irradiation Part number
Production quality inspections Lot number
Production integrity testing Expiration date
Labels Irradiation dose
Deviations Confirmation USP and EP testing of film
Certificate of analysis
7
Introduction
u Quality control and assurance
Validation
A BPC Validation Master Plan has been developed for all Thermo Scientific BPC products in
compliance with the concepts of cGMP for medical devices. Meaningful product validations are
designed to demonstrate compliance with release criteria and product claims. Process validation
evaluates manufacturing conditions as well as product cleanliness and consistency.
Process qualification is performed when a new product or change in a manufacturing process are
introduced. This consists of a production build and validation testing to verify that the product meets
the specification acceptance criteria.
Sterility assurance
Validation of the gamma irradiation Sterility Assurance Level (SAL) for the BPC products is
performed on a quarterly basis as per ANSI/AAMI/ISO 1137: 2006 parts 1, 2 and 3 guidelines.
It involves testing a “worst-case” BPC assembly. The current production irradiation dose of
25–40 kGy provides an SAL of 10-6. The dose audit performed follows the VDmax method outlined in
ANSI/AAMI/ISO 1137:2006.
Endotoxin and particulate

Process validations and monitoring have been established for endotoxin and particulate for the
manufacture of BPC systems. Particulate samples from the fluid path of a worst-case BPC assembly
are tested according to USP 788: Light Obscuration Particle Count Test, and endotoxin testing is
performed to USP 85 in conjunction with bioburden testing.
EMA/410/01 rev. 3 compliance

Due to heightened concerns over animal-origin components, we have taken a leadership role to
compile vendor certificates of origin on each standard component.
These certifications not only provide information as to whether a given component is of animal
origin, but also certify whether the component comes in contact with animal-derived materials
in synthesis, purification, or other manufacture. Supplier statements also contain information on
the species, tissue, and country of origin of any animal-derived substance along with supplier
information on purification or manufacturing steps that would help reduce risk of adventitious
animal-origin agents.
A Component Information Summary (CIS) is available on demand for each BPC product. This lists
the NAO status and EMA/410 compliance of each product contact standard component.
Introduction
BPC validation documentation
Complex BPCs forming the single-use part of systems such as the Thermo Scientific™ S.U.B.,
S.U.F. and S.U.M. are supported by an “information package” covering important information
regarding their testing and design. The document is product-specific rather than lot-specific and is
supplied in electronic format. It is organized into two sections:
1. Materials guide—details of testing protocols and results obtained
2. Performance and functional testing—an overview of engineering design, testing
and test results of individual components as well as of the complete assembly
BPC system hardware documentation

The hardware systems of the S.U.B., S.U.F. and S.U.M. are supplied with an Equipment Turnover
Package (ETP) in electronic format with key supporting information including: top level hardware
drawings, component/instrument manuals, utility requirements, recommended maintenance, torque
wrench operating instructions, warranty statement, detailed electrical panel drawings and electrical
schematics.
A Premium ETP is also available on request for an additional fee and includes: Factory Acceptance
Test (FAT) Checklist, Site Acceptance Test (SAT) Protocol, and Weld and Passivation Certificates.
9
Film selection guide
u Thermo Scientific CX5-14 Film
Thermo Scientific™ CX5-14™ Film is a five-layer, 14 mil cast film produced in a cGMP facility.
The outer layer is a polyester elastomer coextruded with an ethyl vinyl alcohol (EVOH) barrier layer
and an ultra-low density polyethylene product contact layer.
CX5-14 Film is manufactured using no animal-derived components.
0.8 Property Test protocol Average values

Polyester
0.9 Physical data
Tie
1.0 Tensile strength ASTM D882 2302 psi 15.9 MPa
EVOH Elongation ASTM D882 526%
0.9
Tie Yield strength ASTM D882 1479 psi 10.2 MPa
2% secant modulus ASTM D882 43 ksi 296 MPa
PE
Toughness ASTM D882 267 lbf-in 3.0 kN-cm
Dimensions in mil
Puncture resistance Internal 160 lbf-in 1.8 kN-cm
Seam strength* ASTM D882 31 lbf-in 54 N/cm
2 2
O2 transmission rate ASTM D3985 0.035 cc/100 in /day 0.547 cc/m /day
0% RH outside,
10.4 100% RH inside, 23ºC
2 2
CO2 transmission rate Mocon 0.157 cc/100 in /day 2.43 cc/m /day
0% RH outside,
100% RH inside, 23ºC
2 2
Moisture vapor ASTM F1249 0.020 g/100 in /day 0.313 g/m /day
transmission rate 0% RH outside,
Haze ASTM D1003 59%
(outside dry/inside wet)
Glass transition ASTM E1640 -29ºF -34ºC
temperature
Schematic cross-section
Film gauge 0.014 in 356 µm
Film contact material Polyethylene
Temperature range† -112 to 140ºF -80 to 60ºC
Sterilizable 5.0 Mrad 50 kGy
Biocompatibility data (post-gamma irradiation, >50 kGy)
USP Class VI USP <88> Pass
Cytotoxicity USP <87> Pass
Bacterial endotoxin USP <85> 0.005 EU/mL
Heavy metals USP <661> <1 ppm
Buffering capacity USP <661> <1 mL
Schematic 3D view Non-volatile residue USP <661> <1 mg

Residue on ignition USP <661> <1 mg
Hemolysis ISO 10993-4 Nonhemolytic
Appearance EP <3.2.2.1> Pass
Acidity and alkalinity EP <3.2.2.1> Pass
Leachables and extractables testing
Absorbance EP <3.2.2.1> Pass
Extensive testing has been performed on the
Reducing substances EP <3.2.2.1> Pass
CX range of films using standard solutions.
Information is available upon request. Transparency EP <3.2.2.1> Pass
All tests run post-gamma irradiation unless otherwise noted.
*Data generated pre-gamma irradiation.
†Sub-zero conditions require proper support and handling.
Film selection guide
u Thermo Scientific CX3-9 Film
Thermo Scientific™ CX3-9 Film is a three-layer, 9 mil cast film produced in a cGMP
facility. The outer layer is a polyester elastomer coextruded with an ultra-low density
polyethylene product contact layer.
CX3-9 Film is manufactured using no animal-derived components.
Polyester 0.8 Property Test protocol Average values

Tie 0.9 Physical data
Tensile strength ASTM D882 4300 psi 30 MPa
PE
Elongation ASTM D882 1100%
Dimensions in mil Yield strength ASTM D882 900 psi 6 MPa
Toughness ASTM D882 435 lbf-in 4.9 kN-cm
Puncture resistance Internal 85 lbf-in 0.94 kN-cm
7.3 Seam strength ASTM D882 13 lbf-in 23 N/cm
Moisture vapor ASTM F1249 0.06 g/100 in2/day 0.9 g/m2/day
Film gauge 0.009 in 229 µm
Temperature range* -112 to 140ºF -80 to 60ºC
Sterilizable 5.0 Mrad 50 kGy
Biocompatibility data (post-gamma irradiation, >50 kGy)
Schematic cross-section
USP Class VI USP <88> Pass
Cytotoxicity USP <87> Pass
Bacterial endotoxin USP <85> <0.005 EU/mL
Heavy metals USP <661> <1 ppm
Buffering capacity USP <661> <1 mL
Non-volatile residue USP <661> <1 mg
Residue on ignition USP <661> <1 mg
Hemolysis ISO 10993-4 Pass
Absorbance EP <3.2.2.1> 0.0055 units
Reducing substances EP <3.2.2.1> <0.1 mL
Transparency EP <3.2.2.1> Pass
Schematic 3D view *Sub-zero conditions require proper support and handling.
11
Film selection
cell culture
guide
u Thermo Scientific Aegis5-14 Film
Thermo Scientific™ Aegis5-14™ Film is a five-layer, 14 mil cast film produced in a cGMP facility.
The outer layer is a polyester elastomer coextruded with an ethyl vinyl alcohol (EVOH) barrier layer
and an ultra-low density polyethylene product contact layer.
Aegis5-14 Film is manufactured using no animal derived components.
Polyester 0.8 Property Test protocol Average values
Tie 0.9 Physical data

Tensile strength ASTM D882 2276 psi 15.7 MPa
EVOH 1.0 Elongation ASTM D882 507%
Tie 0.9 Yield strength ASTM D882 1494 psi 10.3 MPa
PE Toughness ASTM D882 252 in-lb 2.85 kN-cm
Puncture resistance Internal 173 in-lb 1.95 kN-cm
Dimensions in mil Seam strength* ASTM D882 30lbf/in 53 N/cm
O2 transmission rate ASTM D3985 0.032 cc/100 in2/day 0.491 cc/m2/day
0% RH outside,
CO2 transmission rate Mocon 0.177 cc/100 in2/day 2.737 cc/m2/day
0% RH outside,
10.4 Moisture vapor ASTM F1249 0.022 g/100 in2/day 0.34 g/m2/day
Haze ASTM D1003 (outside 54%
dry/inside wet)
Glass transition temperature ASTM E1640 -29ºF -34ºC
Film gauge 14 mil 356 µm
Temperature range† -112 to 140ºF -80 to 60ºC
Sterilizable 5.0 Mrad/50 kGy
Biocompatibility data (post gamma irradiation, >50 kGy)
USP class VI USP <88> Pass
Schematic Cross-Section Cytotoxicity USP <87> Pass
Bacterial endotoxin USP <85> 0.005 EU/mL
Heavy metals USP <661> <1ppm
Buffering capacity USP <661> <1mL
Non-volatile residue USP <661> <1mg
Residue on ignition USP <661> <1mg
Hemolysis ISO 10993-4 Nonhemolytic
Absorbance EP <3.2.2.1> Pass
Reducing substances EP <3.2.2.1> Pass
Schematic 3D View Transparency EP <3.2.2.1> Pass
All tests run post-gamma irradiation unless otherwise noted.
*Data generated pre-gamma irradiation.
†Sub-zero conditions require proper support and handling.
Leachables and extractables testing
Extensive testing has been performed on Aegis
Film using standard solutions. Information is
available upon request.
Tubing selection guide
u Thermo Scientific tubing selection guide
Although it is not practical to include the complete range of commercially

available tubing in the Thermo Scientific™ Standard Component Library,
a list of sizes for the key types used in the biopharmaceutical industry are
represented.
Key factors in selection of tubing for inclusion in a BPC systems are:
• Pumping characteristics—Peristaltic pumping is the most common method so the

flow rate and pump life of tubing in this system are important. More information is
provided on Data Sheet 016.
• Clarity—Most but not all of the tubing types are clear. The ability to see tubing
contents is normally important.
• Method of connection—Most connection systems involve attaching the tubing to
a hose barb in a standard size. Tubing welders and sealers require specific types of
tubing.
• Supporting data—Regulatory compliance, ADCF status and extractables studies
are often required. A database of information on all standard tubing is available. More
details are provided in Data Sheet 016.
• Economics—Different tubing types, bore sizes and wall thicknesses can vary widely
in cost. In general, the larger the bore size and wall thickness, the more expensive the
tubing.
• Sterilization method—It is standard for tubing used on BPCs to be suitable for
irradiation.
Thermo Scientific tubing for BPC systems available
Type Description Use when:
C-Flex™ This is a common choice of tubing for use in standard and General purpose liquid handling is required.
custom BPC systems due to its overall suitability. Three Also with tubing welding and sealing
formulations are included: clear, opaque and ADCF. Its main equipment.
advantages over silicone are increased chemical resistance and
thermoplastic characteristics.
Platinum-cured silicone A common choice of silicone based on multi-sourcing is General purpose liquid handling is required.
recommended to maximize range of sizes, availability and cost.
Silicone tubing in a range of sizes from suppliers such as
Cole-Parmer™, Saint-Gobain™ and Watson-Marlow™ is
also available.
PVC Medical grade PVC tubing is included in the Standard Heat/radio frequency sealing or very high
Component Library for historical reasons and was widely used clarity are required.
before other alternatives like C-Flex became available.
PharMed™ This tubing has a longer pump life than C-Flex or silicone. Due Long pump life is required.
to its cost and opacity it is normally used only in small sections
of tubing intended to be loaded into a peristaltic pump. The
remaining tube length on the BPC would be C-Flex or silicone.
PharmaPure™ This is supplied by Saint-Gobain™ as an alternative to Long pump life is required.

PharMed™ tubing with ultra-low particulate spallation.
13
Tubing selection
cell culture
guide
The tables below are intended to outline major features, types and sizes of tubing available. The tables
below also include references to Thermo Scientific™ Masterflex™ tubing size numbers. Standard BPC
systems are normally supplied with C-Flex or platinum-cured silicone tubing because of their suitable technical
and economic characteristics.
Product Weld Animal-derived Extractable Gas perme- Autoclave Peristaltic Binding Clarity Sterilization method
description and seal components profile ation tolerance pump life
capability
C-Flex 374 Yes No Good Very low Fair Good Low Clear Gamma Irr.
ADCF
C-Flex clear Yes No Good Very low Fair Good Low Clear Gamma Irr.
formulation
082
C-Flex opaque Yes No Good Very low Fair Very good Low Opaque Gamma Irr.
formulation
072
Silicone, Tygon No No Excellent Moderate Excellent Fair Low Clear Autoclave or Gamma Irr.
3350
Silicone, No No Very good Moderate Excellent Fair Low Clear Autoclave or Gamma Irr.
SPT-50
PharMed BPT Yes Yes Good Very low Good Excellent Low Opaque Autoclave or Gamma Irr.
Inner diameter x outer Pump Default Clear Opaque ADCF PVC PharMed PharmaPure
diameter sizes available size* silicone C-Flex C-Flex C-Flex
3.2 mm x 6.4 mm (1/8 in x ¼ in) 16 X X X X X X X
3.2 mm x 4.8 mm (1/16 in x 3/16 in) X
4.8 mm x 7.9 mm (3/16 in x 5/16 in) 25 X X X X X X X
4.8 mm x 9.5 mm (3/16 in x 3/8 in) 15 X X X X X X
6.4 mm x 9.5 mm (¼ in x 3/8 in) 17 X X X X X X
6.4 mm x 11.1 mm (¼ in x 7/16 in) 24 X X X X X X
6.4 mm x 12.7 mm (¼ in x ½ in) 26 X X X X X X X
7.9 mm x 12.7 mm (5/16 in x ½ in) X X
9.5 mm x 12.7 mm (3/8 in x ½ in) X X X X X X
9.5 mm x 14.3 mm (3/8 in x 9/16 in) X
12.7 mm x 15.9 mm (½ in x 5/8 in) X X
12.7 mm x 19 mm (½ in x ¾ in) 82 X X X X X X
12.7 mm x 17.5 mm (½ in x 11/16 in) 19 X X X X X X
12.7 mm x 22.2 mm (½ in x 7/8 in) X
15.9 mm x 22.2 mm (5/8 in x 7/8 in) X X X X
19 mm x 25.4 mm (¾ in x 1 in) 90 X X X X X X
19 mm x 28.6 mm (¾ in x 1 1/8 in) X X X
19 mm x 31.8 mm (¾ in x 1¼ in) X X X
22.2 mm x 28.6 mm (7/8 in x 1 1/8 in) X X X
22.2 mm x 31.8 mm (7/8 in x 1¼ in) X
25.4 mm x 31.8 mm (1 in x 1¼ in) X X X X
25.4 mm x 34.9 mm (1 in x 1 3/8 in) 92 X X
*Pump sizes listed are for Masterflex tubing pump systems. I.D./O.D. dimensions may vary from Masterflex brand tubing. Users should test product to determine suitability for use in their
application.
Connection selection
cell culture
guide
u Thermo Scientific connection system selection guide
Luer connectors Method of connection: Push male and female together and twist to secure the connection.
Options: Luer connectors are available in different materials and barb sizes.
Advantages: Simple to use.
Limitations: Limited to ¼ inch barb sizes and not 100% secure.
Use when: Small volume aseptic connections are required and can be made in a Class 100
area.
Type Material Hose barb sizes available
luer lock Kynar™ 3.18 mm (1/8 in), 6.35 mm (¼ in)
Male luer lock Female luer lock luer lock Polypropylene 3.18 mm (1/8 in), 6.35 mm (¼ in)
connector connector
CPC quick connects Method of connection: Simply push male and female together until they click.
Options: Available in different materials and sizes. Includes HFC series with integral shut-off valve.
More information is available in Data Sheet 050.
Advantages: Quick, easy and secure to use.
Limitations: Requires Class 100 or better to make an aseptic connection.
Use when: Secure aseptic connections are required and can be made in a Class 100 area.
MPC series Polycarbonate 9.53 mm (3/8 in), 12.67 mm (½ in)
Male (insert) Female (body)
MPX series Polysulfone 12.7 mm (½ in)
MPU series Polysulfone 19 mm (¾ in)
HFC39 Polysulfone 6.35 mm (¼ in), 9.52 mm (3/8 in)
Tri-clamp Method of connection: Place flat flanges together with a gasket between them and secure using
a clamp.
Options: Available in 1½ inch and ¾ inch (also known as mini tri-clamps) tri-clamp (TC) flange
sizes in a range of different materials. SterilEnz™ fittings have the advantage that the tri-clamp
connection is protected by an integral bag. More information is available in Data Sheet 051.
Advantages: Largest barb size of all available connectors on BPCs. Very secure connection.
Limitations: Not suitable for making aseptic connections.
SterilEnz fitting Use when: A BPC has to be connected to a vessel or equipment with a tri-clamp port and sterility
is not required. Normally in downstream applications.
SterilEnz mini TC Polyethylene 12.7 mm (½ in)
SterilEnz TC Polyethylene 12.7 mm (½ in), 19 mm (¾ in)
Mini TC Polypropylene 3.18 mm (1/8 in), 6.35 mm (¼ in), 9.52 mm (3/8 in), 12.7 mm (½ in)
Mini tri-clamp
Mini TC Kynar 3.18 mm (1/8 in), 6.35 mm (¼ in), 9.52 mm (3/8 in), 12.7 mm (½ in)
TC Polypropylene 3.18 mm (1/8 in), 6.35 mm (¼ in), 9.52 mm (3/8 in), 12.7 mm (½ in), 25.4
mm (1 in)
TC Kynar 3.18 mm (1/8 in), 6.35 mm (¼ in), 9.52 mm (3/8 in), 12.7 mm (½ in), 25.4
mm (1 in)
Tri-clamp
15
Connection selection guide
Aseptic connection devices (ACD) Method of connection: Similar to Tri-clamp; connection faces vary by model.
Options: Pall™ Kleenpak™, ReadyMate™ Disposable Aseptic Connector (DAC) and CPC™
AseptiQuik™ connectors. More information is available in Data Sheet 053.
Advantages: Allow aseptic connections to be made anywhere without dependence on an
instrument.
Limitations: Not the most economical option.
Use when: Aseptic connections are required and a Class 100 area is not available.
Pall Kleenpak connection CPC AseptiQuik Type Material Hose barb sizes available
connection (X)
Pall Kleenpak Polycarbonate 6.35 mm (¼ in), 9.52 mm (in), 12.7 mm (½ in)
ReadyMate DAC Polycarbonate/Silicone 6.35 mm (¼ in), 9.52 mm (3/8 in), 12.7 mm (½ in), 19 mm
(¾ in)
CPC AseptiQuik Polycarbonate/Silicone 3.18 mm (1/8 in), 6.35 mm (¼ in), 12.7 mm (½ in), 9.5 mm
(3/8 in), 19 mm (¾ in)
CPC AseptiQuik Method of connection: Automatic instrument cuts and joins two tubes aseptically to form a
connection (S)
connection.
Tubing welder Options: Requires either C-Flex or PharMed tubing attached to the BPC. Since a terminal
connector is not required the normal end treatment is an end plug.
Advantages: Flexibility.
Limitations: Requires an instrument to make the connection.
Use when: Aseptic connections are required and a Class 100 area is not available.
End plug Polypropylene 3.18 mm (1/8 in), 6.35 mm (¼ in), 9.52 mm (3/8 in), 12.7 mm (½ in), 19 mm
(¾ in)
Tubing in welder jaws Female (body)
End plug Kynar 3.18 mm (1/8 in), 6.35 mm (¼ in), 9.52 mm (3/8 in), 12.7 mm (½ in)
Method of connection: Same as Tri-clamp where the mating flange will be part of a SIP supply
assembly.
Steam in Place Options: See Data Sheet 052 for available options.
Advantages: Validatable sterile connection is possible.
Limitations: Slow and requires clean steam.
Use when: A BPC sterile connection of a BPC to a stainless steel vessel with a tri-clamp port is
required.
Unitized mini TC Silicone 9.52 mm (3/8 in), 12.7 mm (½ in)
CPC Steam-thru Unitized TC Silicone 9.52 mm (3/8 in), 12.7 mm (½ in),
connection 19 mm (¾ in)
CPC Steam-Thru™ (TC and mini TC) Polysulfone 9.52 mm (3/8 in), 12.7 mm (½ in)
CPC Steam-Thru II Polysulfone 9.52 mm (3/8 in), 12.7 mm (½ in)
Millipore™ Lynx™ ST connectors Polyetherimide/ 6.35 mm (¼ in), 12.7 mm (½ in)
(TC and mini TC) silicone
Sample Ports Method of connection: Insert syringe into port to remove sample.
Options: Similar devices are available—SmartSite™ connector and Clave™ connector.
Advantages: No needle required.
Limitations: Flow rate.
Use When: Samples of 50 mL or less are required.
SmartSite Acrylic/polyurethane/silicone N/A
SmartSite connection Clave Information not available N/A
Filter selection
cell culture
guide
u Thermo Scientific filter selection guide
Filtration is an important step in bioprocessing and a variety of filters are

available through the Thermo Scientific Standard Component Library to
meet your needs. Our open architecture approach to components
enables us to provide customers a broad choice of both filters and
manufacturers, including opportunities for dual sourcing.
Some major characteristics relevant to filter selection:
• Membrane material—The major types of filter material used in bioprocessing today are
polyethersulfone (PES) and polyvinylidene (PVDF)
• Membrane size—Required filter pore size will be influenced by the size of the materials
being filtered, as well as the level of filtration the process necessitates
• Affinity to water—The presence of aqueous solution impacts the type of filter that may
be most advantageous to your process
• Application—Sequence within your process where filtration takes place and the type of
material being filtered have implications for filter selection
A sample of the filters available through our comprehensive Standard Component Library include:
Supplier Type Material Final filter pore sizes (µm)
PROPOR™ SG, DOMICAP™ capsule PES 0.1, 0.2

Dominick
Hunter™ PROPOR™ SG, MURUS capsule PES 0.1, 0.2
SteriLUX™, UltraCap™ capsule PVDF 0.1, 0.2
SteriLUX, capsule PVDF 0.2
Meissner™
STyLUX™, UltraCap capsule PES 0.1, 0.2
STyLux, capsule PES 0.1, 0.2
Fluorodyne™ II DJL, Kleenpak PVDF 0.1
capsule
Pall Fluorodyne II DFL, Kleenpak capsule PVDF 0.2
Supor EKV, Kleenpak capsule PES 0.2
Millipak™ filter unit PVDF 0.1, 0.2
Millipore Opticap™ XL capsule PVDF 0.1, 0.2
Opticap XL capsule PES 0.1, 0.2
Sartorius™ Sartopore™ 2 capsule PES 0.1, 0.2
A variety of factors unique to your process may influence your choice of filter, in addition to those
considerations listed. Contact your Thermo Scientific product sales representative to assist with any
questions or for assistance with selecting the best filter for your process needs.
17
Fluid transfer assemblies
u Thermo Scientific fluid transfer assemblies
Building a custom fluid transfer system? We are your ultimate customer

partner for single-use fluid transfer assemblies.
• A complete end-to-end solution provider

A single point of contact for design, manufacturing and service
• Your trusted guide for bioprocess applications

Components from our superior Standard Components Library
• Delivering the benefits of single-use technology, customized

Systems that integrate within your existing processes
Our fluid transfer assemblies are delivered sterile and ready-to-use

for your bioprocess operations:
• Media and buffer transfer • Filtration and purification process connection

• Product sampling • Bulk product and final fill
• Bioreactor feed transfer • Dispensing and aliquoting
• Harvest collection
Contact your Thermo Scientific representative for more information.
Associated products
• 2D BPC Systems (Pg. 19) • HyPerforma S.U.B. (Pg. 52) • Harvestainer Microcarrier Separation BPC and
• 3D BPC Systems (Pg. 23) • HyPerforma S.U.F. (Pg. 57) System (Pg. 26)
• Tank Liners (Pg. 28) • HyPerforma S.U.M. (Pg. 43)
2D BPC systems
u 2D Labtainer BPC systems
Key features
• 2D Labtainer BPC systems are 2 panel, pillow-style BPCs

• Chambers are constructed from either CX5-14 Film or our premium Aegis5-14 Film
• Labtainer BPC systems have 2–3 edge ports along one end with a handle on the
opposing end
• 2-port Labtainer BPCs are available in sizes from 50 mL to 2,000 mL
• 3-port Labtainer BPCs are available in sizes from 2 L to 50 L
• Line sets can be customized for easy integration with existing process operations and
equipment
• Labtainers can be customized into multi-container manifold configurations
• Labtainers can be added as sample container adjuncts to 2D or 3D BPCs
Applications
• Harvest from bioreactors or fermentors

• Feed into bioreactors or fermentors
• Sample collection from bioreactors or fermentors
• Buffer preparation and storage
• Culture media preparation and storage
• Process liquid preparation and storage
• Chromatography feed and fraction collection
• Harvest, storage and transport of bulk drug product and bulk drug precursors
• Waste collection
Supporting information
• Aegis5-14 Film Fact Sheet

• CX5-14 Film Fact Sheet
Associated products
• 3D BPC Systems (Pg. 23)

• HyPerforma S.U.B. (Pg. 52)
• HyPerforma S.U.F. (Pg. 57)
• HyPerforma S.U.M.(Pg. 43)
19
2D BPC systems
2D BPCs, pillow design, edge ports
2 ports 2 ports 2 ports

pack of 10 pack of 10 pack of 10
Line 1 Female luer lock connection, Line 1 Female luer lock connection, Line 1 Female luer lock connection,
polypropylene Tubing: C-Flex polypropylene Tubing: C-Flex polypropylene
length: 30 cm (12 in) length: 30 cm (12 in) No tubing
ID x OD: 3.2 mm x 6.4 mm ID x OD: 3.2 mm x 6.4 mm
(1/8 in x ¼ in) (1/8 in x ¼ in)
Line 2 Male luer lock connection, Line 2 Male luer lock connection,
polypropylene Tubing: C-Flex Line 2 MPC insert, polycarbonate polypropylene
length: 30 cm (12 in) No tubing No tubing
ID x OD: 3.2 mm x 6.4 mm
(1/8 in x ¼ in)
Cat. No. Size Dimensions (L x W) Cat. No. Size Dimensions (L x W) Cat. No. Size Dimensions (L x W)
Aegis5-14 Film: Aegis5-14 Film: Aegis5-14 Film:
SH30961.11 SH31048.11 SH31050.11
50 11.7 cm x 13.7 cm 50 11.7 cm x 13.7 cm 50 11.7 cm x 13.7 cm
mL (4.6 in x 5.4 in) mL (4.6 in x 5.4 in) mL (4.6 in x 5.4 in)
CX5-14 Film: CX5-14 Film: CX5-14 Film:
SH30658.11 SH30662.11 SH30657.11

SH30961.12 SH31048.12 SH31050.12
SH30658.12 SH30662.12 SH30657.12

SH30961.13 SH31048.13 SH31050.13
250 19.1 cm x 15 cm 250 19.1 cm x 15 cm 250 19.1 cm x 15 cm
SH30658.13 SH30662.13 SH30657.13

SH30961.14 SH31048.14 SH31050.14
SH30658.14 SH30662.14 SH30657.14

SH30961.15 SH31048.15 SH31050.15
29.7 cm x 20.1 cm 29.7 cm x 20.1 cm 29.7 cm x 20.1 cm
1L 1L 1L
(11.7 in x 7.9 in) (11.7 in x 7.9 in) (11.7 in x 7.9 in)
SH30658.15 SH30662.15 SH30657.15

SH30961.16 SH31048.16 SH31050.16
2L 2L 2L
SH30658.16 SH30662.16 SH30657.16
No. per case: 10 (all above) No. per case: 10 (all above) No. per case: 10 (all above)
2D BPC systems
BPCs, edge ports

single pack single pack single pack
Line 1 Male luer lock connection, Line 1 MPC insert, polycarbonate Line 1 MPC insert, polycarbonate
polypropylene Tubing: C-Flex Tubing: C-Flex Tubing: C-Flex
length: 30 cm (12 in) length: 61 cm (24 in) length: 30 cm (12 in)
ID x OD: 6.4 mm x 9.5 mm ID x OD: 9.5 mm x 15.9 mm ID x OD: 9.5 mm x 12.7 mm
(¼ in x 3/8 in) (3/8 in x 5/8 in) (3/8 in x ½ in)
Line 2 Female luer lock connection, Line 2 MPC body, polycarbonate Line 2 MPC insert, polycarbonate
polypropylene Tubing: C-Flex Tubing: C-Flex Tubing: C-Flex
length: 30 cm (12 in) length: 61 cm (24 in) length: 30 cm (12 in)
(¼ in x 3/8 in) (3/8 in x 5/8 in) (3/8 in x ½ in)
Line 3 Female luer lock connection, Line 3 Female luer lock connection, Line 3 Plugged
polypropylene polypropylene Tubing: C-Flex No tubing
Tubing: C-Flex length: 61 cm (24 in)
length: 30 cm (12 in) ID x OD: 3.2 mm x 6.4 mm
ID x OD: 3.2 mm x 6.4 mm (1/8 in x 1/4 in)
(1/8 in x ¼ in)
Cat. No. Size Dimensions (L x W) Cat. No. Size Dimensions (L x W) Cat. No. Size Dimensions (L x W)
SH31049.05 SH30963.05 SH30965.05
29.5 cm x 31 cm 29.5 cm x 31 cm 29.5 cm x 31 cm
2L 2L 2L
SH30713.05 SH30712.05 SH30714.05

SH31049.01 SH30963.01 SH30965.01
5L 5L 5L
SH30713.01 SH30712.01 SH30714.01

SH31049.02 SH30963.02 SH30965.02
62.2 cm x 30 cm 62.2 cm x 30 cm 62.2 cm x 30 cm
10 L 10 L 10 L
SH30713.02 SH30712.02 SH30714.02

SH31049.03 SH30963.03 SH30965.03
20 L 20 L 20 L
(25.8 in x 17 in) (25.8 in x 17 in) (25.8 in x 17 in)
SH30713.03 SH30712.03 SH30714.03

SH31049.04 SH30963.04 SH30965.04
50 L 50 L 50 L
(32.5 in x 23 in) CX5-14 Film:
(32.5 in x 23 in) (32.5 in x 23 in)
CX5-14 Film: CX5-14 Film:
SH30713.04 SH30712.04 SH30714.04
21
cellsystems
2D BPC culture
BPCs, edge ports 2D pillow design

with panel ports
3 ports 3 ports
single pack single pack
Line 1 MPC insert, polycarbonate Line 1 MCP insert, polycarbonate

No tubing Tubing: C-Flex
Line 2 MPC insert, polycarbonate length: 61 cm (24 in)
No tubing ID x OD: 9.5 mm x 15.9 mm
(3/8 in x 5/8 in)
Line 3 Injection port
No tubing Line 2 MPC body, polycarbonate
Tubing: C-Flex
length: 61 cm (24 in)
Cat. No. Size Dimensions (L x W) ID x OD: 9.5 mm x 15.9 mm
(3/8 in x 5/8 in)
Aegis5-14 Film:
SH31005.05 Line 3 Female luer lock connection,
29.5 cm x 31 cm polypropylene Tubing: C-Flex
2L
(11.6 in x 12.2 in)
CX5-14 Film: length: 66 cm (26 in)
SH30709.05 ID x OD: 3.2 mm x 6.4 mm
(1/8 in x ¼ in)
Aegis5-14 Film:
SH31005.01
37.6 cm x 33.3 cm
5L
CX5-14 Film: (14.8 in x 13.1 in) Cat. No. Size Outer container
SH30709.01
Cat. No.
Aegis5-14 Film: CX5-14 Film: 50 L SV50076.02

SH30667.01
SH31005.02 62.2 cm x 30 cm
10 L
(24.5 in x 11.8 in) CX5-14 Film:
CX5-14 Film: SH30667.02 100 L SV50076.03
SH30709.02
Aegis5-14 Film: CX5-14 Film:

SH30667.03 200 L SV50076.04
SH31005.03
65.5 cm x 43.2 cm
20 L
CX5-14 Film:
(25.8 in x 17 in)
SH30709.03
Aegis5-14 Film:
SH31005.04 Aegis5-14 Film equivalents for this product
82.6 cm x 58.4 cm
50 L
CX5-14 Film:
(32.5 in x 23 in) are available as custom configurations.
SH30709.04
3D BPC
cellsystems
culture
u 3D BPC systems

3D square tube design with top dispense
Line 1 MPC insert, polycarbonate Line 1 MPC insert, polycarbonate Line 1 MPX insert, polycarbonate
Tubing: C-Flex Tubing: C-Flex Tubing: length: 121.9 cm (48 in)
length: 45.7 cm (18 in) length: 121.9 cm (48 in) ID x OD: 12.7 mm x 19.1 mm
ID x OD: 9.5 mm x 15.9 mm ID x OD: 9.5 mm x 15.9 mm (½ in x ¾ in), with dip tube length
(3/8 in x 5/8 in) (3/8 in x 5/8 in) with dip tube length dependant on size
dependant on size Line 2 Male luer lock conection,
Line 2 MPC body, polycarbonate
Line 2 Male luer lock connection, polypropylene Tubing: length:
Tubing: C-Flex
polypropylene Tubing: C-Flex 121.9 cm (48 in)
length: 45.7 cm (18 in)
length: 121.9 cm (48 in) ID x OD: 6.4 mm x 9.5 mm
ID x OD: 9.5 mm x 15.9 mm
ID x OD: 6.4 mm x 9.5 mm (¼ in x 3/8 in)
(3/8 in x 5/8 in)
(¼ in x 3/8 in ) Line 3 Female luer lock connection,
Cat. No. Size Outer container Line 3 Female luer lock connection, polypropylene Tubing: length:
Cat. No. polypropylene Tubing: C-Flex 121.9 cm (48 in)
length: 121.9 cm (48 in) ID x OD: 6.4 mm x 9.5 mm
Aegis5-14 Film:
SH30964.01
50 L ID x OD: 6.4 mm x 9.5 mm (¼ in x 3/8 in)
dip tube:
38 cm
SV50076.02 (¼ in x 3/8 in) Line 4 MPX body, polycarbonate
CX5-14 Film: Tubing: length: 121.9 cm (48 in)
SH30649.01
(15 in) Line 4 MPC body, polycarbonate
Tubing: C-Flex ID x OD: 12.7 mm x 19.1 mm
No. per case: 8 (all above) length: 121.9 cm (48 in) (½ in x ¾ in)
Aegis5-14 Film: ID x OD: 9.5 mm x 15.9 mm dip tube length: 30.5 cm (12 in)
SH30964.02 100 L (3/8 in x 5/8 in)
dip tube:
CX5-14 Film: 71 cm
SV50076.03 dip tube length: 30.5 cm (12 in) Cat. No. Size Outer container
SH30649.02 (28 in) Cat. No.
Cat. No. Size Outer container
No. per case: 10 (all above) Cat. No. Aegis5-14 Film: 50 L
dip tube:
Aegis5-14 Film: SV50076.02
Aegis5-14 Film: 50 L 38 cm
200 L SH30966.01 dip tube: CX5-14 Film: (15 in)
SH30964.03 SH30653.01
dip tube: SV50076.04 38 cm SV50076.02
CX5-14 Film:
CX5-14 Film: 81 cm SH30651.01 (15 in)
SH30649.03 (32 in) No. per case: 6 (all above)
No. per case: 5 (all above)
No. per case: 5 (all above) Aegis5-14 Film: 100 L
Aegis5-14 Film: 100 L
SH30966.02 dip tube: dip tube:
SV50076.03
71 cm SV50076.03 71 cm
CX5-14 Film:
CX5-14 Film: (28 in) SH30653.02 (28 in)
SH30651.02
No. per case: 6 (all above) No. per case: 4 (all above)
Aegis5-14 Film: 200 L Aegis5-14 Film: 200 L

SH30966.03 dip tube: dip tube: SV50076.04
81 cm SV50076.04 81 cm
SH30651.03 (32 in) (32 in)
SH30653.03
23
3D BPC systems
u 3D BPC systems
3D square tube design with bottom port dispense

For use with conical, bottom drain drums
Line 1 MPX body, polycarbonate Line 1 19.1 mm (¾ in) tri-clamp, Line 1 MPC insert, polycarbonate
Tubing: C-Flex polyethylene Tubing: C-Flex, length:
length: 45.7 cm (18 in) Tubing: length: 45.7 cm (18 in) 121.9 cm (48 in) dip tube
(½ in x ¾ in) (½ in x ¾ in) (3/8 in x 5/8 in)
Line 2 Female luer lock connection, Line 2 19.1mm (¾ in) tri-clamp, dip tube length: 30.5 cm (12 in)
polypropylene Tubing: C-Flex polyethylene Line 2 Male luer lock connection,
length: 30.5 cm (12 in) Tubing: length: 45.7 cm (18 in) polypropylene Tubing: C-Flex
ID x OD: 6.4 mm x 9.5 mm ID x OD: 12.7 mm x 19.1 mm length: 121.9 cm (48 in)
(¼ in x 3/8 in) (½ in x ¾ in) ID x OD: 6.4 mm x 9.5 mm
Line 3 19.1mm (¾ in) tri-clamp, (¼ in x 3/8 in)
Line 3 MPX insert, polycarbonate
Tubing: C-Flex polyethylene Line 3 Female luer lock connection,
length: 121.9 cm (48 in) Tubing: C-Flex, polypropylene Tubing: C-Flex
ID x OD: 12.7 mm x 19.1 mm length: 121.9 cm (48 in) length: 121.9 cm (48 in)
(½ in x ¾ in), bottom port ID x OD: 12.7 mm x 19.1 mm ID x OD: 6.4 mm x 9.5 mm
(½ in x ¾ in) (¼ in x 3/8 in)
Cat. No. Size Outer container Line 4 Plugged (no tubing)
Cat. No. Cat. No. Size Outer container
Cat. No. Line 5 MPC body, polycarbonate
Aegis5-14 Film: 50 L SV50076.02 Tubing: length: 121.9 cm (48 in)
SH30967.01 Aegis5-14 Film: 50 L
dip tube: SH30968.01 SV50076.02 ID x OD: 9.5 mm x 15.9 mm
dip tube:
38 cm
38 cm
(3/8 in x 5/8 in)
(15 in)
CX5-14 Film:
SH30650.01
SV50517.04 CX5-14 Film: (15 in) SV50517.04 bottom port, silicone
SH30672.01
No. per case: 6 (all above) Cat. No. Size Outer container
Aegis5-14 Film: 100 L SV50076.03
SH30967.02 Cat. No.
dip tube:
Aegis5-14 Film:
71 cm SH30968.02 100 L SV50076.03 Aegis5-14 Film:
CX5-14 Film: dip tube: SH30969.01 50 L
(28 in) SV50517.05
SH30650.02 71 cm dip tube:
CX5-14 Film: SV50517.04
(28 in) 38 cm
SH30672.02 SV50517.05 CX5-14 Film:
SH30652.01 (15 in)
Aegis5-14 Film: 200 L SV50076.04
SH30967.03
dip tube: No. per case: 4 (all above) Aegis5-14 Film:
81 cm SH30969.02 100 L
CX5-14 Film: (32 in) SV50517.06 dip tube:
SH30650.03 Aegis5-14 Film: SV50076.04 SV50517.05
SH30968.03 200 L 71 cm
dip tube: CX5-14 Film:
SH30652.02 (28 in)
No. per case: 5 (all above) CX5-14 Film: 81 cm
SH30672.03 (32 in) SV50517.06 No. per case: 5 (all above)
No. per case: 5 (all above) Aegis5-14 Film:
SH30969.03 200 L
dip tube: SV50517.06
CX5-14 Film: 81 cm
SH30652.03 (32 in)
BPCs for large volume cell
containers
culture
u BPCs for large volume stainless steel outer container systems
BPCs with bottom drain dispense

4/5 ports 3 ports Associated products
single pack single pack
• HyPerforma S.U.M. (Pg. 43)
• Tank Liners (Pg. 28)
Line 1 MPC insert, polycarbonate Line 1 MPX insert, polycarbonate

Tubing: C-Flex, length: Tubing: C-Flex
121.9 cm (48 in) ID x OD: 9.5 length: 51 cm (20 in)
mm x 15.9 mm (3/8 in x 5/8 in), ID x OD: 12.7 mm x 19.1 mm
dip tube length: 30.5 cm (12 in) (½ in x ¾ in)
Line 2 Male luer lock, polypropylene Line 2 MPX insert, polycarbonate
Tubing: C-Flex, length: Tubing: C-Flex
121.9 cm (48 in) ID x OD: 6.4 length: 51 cm (20 in)
mm x 9.5 mm (¼ in x 3/8 in) ID x OD: 12.7 mm x 19.1 mm
Line 3 Female luer lock, polypropylene (½ in x ¾ in)
Tubing: C-Flex Line 3 MPX insert, polycarbonate
length: 121.9 cm (48 in) Tubing: C-Flex
ID x OD: 6.4 mm x 9.5 mm length: 51 cm (20 in)
(¼ in x 3/8 in) ID x OD: 12.7 mm x 19.1 mm
Line 4 Plugged (½ in x ¾ in), bottom port
No tubing
Line 5 MPC body, polycarbonate Cat. No. Size Outer container
Tubing: C-Flex Cat. No.
length: 121.9 cm (48 in)
ID x OD: 9.5 mm x 15.9 mm Aegis5-14 film:
(3/8 in x 5/8 in) SH31047.01
100 L SV50160.01
bottom port, silicone
CX5-14 Film:
SH30717.01
Cat. No. Aegis5-14 Film:
SH31047.02
Aegis5-14 Film: 200 L SV50160.01
SH30969.04 CX5-14 Film:
500 L SV50160.02 SH30717.02
CX5-14 Film:
SH30652.04 No. per case: 5 (all above)
Aegis5-14 Film: Aegis5-14 Film:

SH30969.05 SH31047.0
1000 L SV50160.03 500 L SV50160.02
CX5-14 Film:
CX5-14 Film:
SH30652.05
SH30717.03
Aegis5-14 Film:
SH30969.06 No. per case: 4 (all above)
1500 L SV50160.04
CX5-14 Film:
SH30652.06
25
Harvestainer BPC systems
u Thermo Scientific Harvestainer microcarrier separation BPC and system
For bioproduction processes using anchorage-dependent cells grown on

microcarriers, the Thermo Scientific™ Harvestainer™ Microcarrier Separation
BPC enables the simple and effective separation of microcarriers from the cell
culture supernatant harvest in a single-step, single-use, completely closed
system. The Harvestainer BPC features a dual chamber system comprising
a 200 L BPC that contains a 25 L pillow style BPC within. This inner BPC, the
Thermo Scientific™ Microbarrier Labtainer™ BPC, maximizes product recovery
and microcarrier retention, with the beads remaining in this BPC while the
supernatant collects inside the outer 200 L BPC.
Key features
• Simple, efficient, single-use microcarrier/harvest separation

• Self-contained closed system helps reduce cross-contamination risk
• One of the most economical options available
• Dual interior Microcarrier Labtainer option and Harvestainer BPC manifolding capability enable
a spectrum of expansion, making the system scalable from process development to full cGMP
commercial production
• Enables consistent pass-through rate from start to finish without decrease or impedance to flow
• Minimal loss in viability or yield post separation
• Minimal set-up and clean-up time
• Full product containment and single-use for easy disposal
• Helps eliminate need for investment in costly equipment
• Thermo Scientific Harvestainer BPC brochure

• Validation Guide for Thermo Scientific Harvestainer BPC
• Microcarrier bead separation and cell harvest using Thermo Scientific Harvestainer
Application Note
• Thermo Scientific Harvestainer BPC animation clip
• Thermo Scientific Harvestainer BPC video
Associated
products
Cat. No. Description Volume Inner BPC

SH30936.01 Harvestainer BPC w/ single inner BPC 200 L 1 x 25 L
No. per Case 5
SH30936.02 Harvestainer BPC w/ dual inner BPC 200 L 1 x 25 L
No. per Case 4
SV50517.29 Polyethylene drum, bottom drain with lid, latches and cap plug
SV50029.03 Drum dolly, 243/8 inches x 71/8 inches (D x H)
Harvestainer BPC systems
Flexibility and scalability
To help ensure added flexibility, the Harvestainer BPC comes in a 200 L size and is available with
single or dual Microbarrier Labtainer BPCs. Multiple Harvestainer BPCs can be linked together in
a manifold assembly, allowing for scale-up from process development to full-scale commercial
production.
Transfer Port
from Bioreactor
Vent
Adherent cells are cultured in a

bioreactor using microcarrier beads
Connect the Harvestainer BPC to the

Microbarrier bioreactor via the transfer port
Labtainer BPC
Drain bioreactor contents directly

into the Harvestainer BPC
Outer BPC
Microcarrier beads are trapped

in the Microbarrier Labtainer BPC,
supernatant passes into the outer BPC
Microbarrier
Beads
Supernatant is ready for the
next processing stage
Harvestainer BPC and microcarriers

are ready for disposal
Bottom Drain Port
Well characterized and customizable

The Harvestainer BPC is composed of well-characterized materials commonly used in single-use BPCs.
Like all Thermo Scientific BPCs, the Harvestainer BPC is scalable and can be customized to readily
integrate with existing equipment. It is designed to maximize product recovery and is built to the same
quality standards found in all Thermo Scientific single-use products.
27
Tank liners
u Tank liners
Key features
• Thermo Scientific tank liners are designed for use with commercially available overhead
mixers (not supplied)
• Removes the need for tank cleaning and helps reduce cycle times
• Chamber constructed of CX3-9 Film (and Aegis5-14 Film for bottom-drain) with dimensions
optimized for Thermo Scientific™ standard drums and commonly used industry standard
cylindrical tanks
• Top entry for standard products for maximum recovery using industry standard connection
systems in unit volumes of 50, 100 and 200 L
• Supplied sterile to minimize bioburden
• Dolly available to provide mobility
Applications
• Hydration of powdered media and buffers
• Pooling of non-sterile solutions and fluids
Supporting information Liners, bottom drain

• CX3-9 Film Fact Sheet 1 port
• BPC Handling Data Sheet 047
• Drums Data Sheet 045
• Tank Liners Data Sheet 008
Line 1 MPX insert, polycarbonate

Liners, top drain Liners, top drain Tubing: C-Flex
For use with cylinderical tanks length: 182.9 cm (72 in)
For use with Thermo Scientific drums
ID x OD: 12.7 mm x 19.1 mm
(½ in x ¾ in)

Cat. No.
Aegis5-14 Film: 50 L SV50076.02
SH30988.01 dip tube:
38 cm
CX3-9 Film: (15 in)
SH30646.01 SV50517.04
Cat. No. Size Outer container Cat. No. Size Outer container Aegis5-14 Film:
SH30988.02 100 L SV50076.03
Cat. No. Cat. No. dip tube:
71 cm
SH30647.01 50 L SV50076.02 SH30647.04 50 L SV30111 CX3-9 Film:
SH30646.02 (28 in) SV50517.05
No. per case: 30 No. per case: 34
SH30647.02 100 L SV50076.03 SH30647.05 100 L SV30112 No. per case: 10 (all above)
No. per case: 26 No. per case: 25 Aegis5-14 Film:
SH30988.03 200 L SV50076.04
SH30647.03 200 L SV50076.04 SH30647.06 300 L SV30114 dip tube:
No. per case: 15 No. per case: 12 CX3-9 Film: 81 cm
SH30646.03 (32 in) SV50517.06
SH30647.07 500 L SV30116
No. per case: 8 No. per case: 8 (all above)
Plastic tanks, drums and accessories
cell culture
Thermo Scientific plastic tanks, drums and accessories
Outer containers
Cylindrical, flat-bottom LLDPE tank with lid

top dispense
Cat. No. Size Dimensions (D x H)
SV30133 19 L 27.9 cm x 38.1 cm (11 in x 15 in)
SV30111 57 L 33 cm x 68.6 cm (13 in x 27 in)
SV30112 113 L 45.7 cm x 76.2 cm (18 in x 30 in)
SV30113 208 L 55.9 cm x 91.4 cm (22 in x 36 in)
SV30114 303 L 61 cm x 121.9 cm (24 in x 48 in)
SV30115 378 L 71.1 cm x 111.8 cm (28 in x 44 in)
SV30116 568 L 78.7 cm x 124.5 cm (31 in x 49 in)
Connection Selection Guide

Flat-bottom LLDPE drum
top dispense, with clamps
SV50076.02 50 L 45 cm x 53 cm (175/8 in x 20¾ in)
SV50076.03 100 L 45 cm x 79 cm (175/8 in x 31¼ in)
SV50076.04 200 L 45.7 cm x 76.2 cm (18 in x 30 in)
Conical LLDPE drum, 1 port, size: 10.2 cm (4 in)

with clamps, use support plate
SV50517.08 50 L 60 cm x 58 cm (23½ in x 23 in)
SV50517.09 100 L 60 cm x 76 cm (23½ in x 29¾ in)
SV50517.10 200 L 60 cm x 114 cm (23½ in x 44¾ in)
29
Plastic tanks, drums and accessories
Thermo Scientific plastic tanks, drums and accessories
Conical LLDPE drum, 1 port, size: 4.5 cm (1.75 in)

with clamps
SV50517.04 50 L 60 cm x 58 cm (23½ in x 23 in)
SV50517.05 100 L 60 cm x 76 cm (23½ in x 29¾ in)
SV50517.06 200 L 60 cm x 114 cm (23½ in x 44¾ in)
Plastic drum dolly

SV50029.03 50, 100, 200, 300 L 62 cm x 18 cm (243/8 in x 71/8 in)
Support plate
round plastic disk needed when using conical LLDPE drum with 4 inch port
Cat. No. Size
SV50102.02 50, 100, 200 L
Large volume rigid plastic support cell
containers
culture
u Large volume rigid plastic support containers and accessories
We have selected and qualified rigid plastic vessels, known as ALLpaQ™

bins, to support our BPC products in helping meet your large volume
liquid storage, handling, and shipping needs.
Key features
• Reusable—Robust design and sturdy construction permits multiple uses

• Convenient—Collapsible walls fold down for easy storage when bins are not in use
• Stackable—Enable extra storage both empty and full
• Bottom drain—True bottom drain options available
• Shippable—Units qualified for shipping with correct dunnage and pack-outs
Applications
• Sterile media and buffer storage and transport within a facility
• Feeding sterile media into bioreactors and buffers into purification systems
• Receiving and storing of harvest from bioreactors
• Shipping sterile bioprocess liquids to another site when proper packing and dunnage
utilized
Associated products

• Tank Liners (Pg. 28)
AllPaq rigid plastic support bins

Cat. No. Size Outer dimensions (W x D x H) Dispensing Material
SV50139.10 250 L 809 mm x 609 mm x 965 mm (31.8 in x 23.9 in x 38 in) Top and bottom dispense polypropylene
SV50139.12 500 L 1,200 mm x 800 mm x 1,040 mm (47.2 in x 31.5 in x 41 in) Top and bottom dispense ABS/HDPE
SV50139.11 600 L 1,155 mm x 1,155 mm x 980 mm (45.5 in x 45.5 in x 38.6 in) Top and bottom dispense polypropylene
SV50139.06 1000 L 1,155 mm x 1,155 mm x 1,294 mm (45.5 in x 45.5 in x 50.9 in) Top and bottom dispense polypropylene
Note: Other sizes and options available on a non-standard basis. For volumes under 500 liters, qualified plastic drums and tanks are recommended.
ALLpaQ 316 stainless steel carts

Cart Cat. No. Size Support bin Cat. No.
SV50139.13 500 L SV50139.12
SV50139.16 1,000 L SV50139.02
SV50139.16 1,000 L SV50139.06
600 L SV50139.11
31
Large volume stainless steel support containers
u Large volume stainless steel outer container systems
Key features
• Thermo Scientific™ HyPerforma™ Smartainer™Systems have chambers constructed

of Thermo Scientific Aegis5-14 Film and CX5-14 Film with dimensions optimized for
Smartainer systems
• Smartainer systems are simple, durable and economic containers constructed in 304
stainless steel
• They are intended for in-house handling applications in critical areas
• Smartainer systems are non-jacketed outer container systems available in unit volumes of
200, 500, 1,000, 1,500 and 2,000 L
• It is a modular system with optional accessories for mobility and BPC handling
• Jacketed versions available in all sizes on a custom basis, for applications where heating or
cooling are required
• Smartainer systems are designed to allow both top and bottom discharge from BPCs
• Standard BPC configurations are available in unit volumes of 100, 200, 500, 1,000 and
1,500 L
Applications
• Sterile media and buffer storage and transport within facility

• Feeding sterile media into bioreactors and buffers into purification systems
• Receiving and storage of harvest from bioreactors
• Not intended for shipping applications
v
Associated products

• Temperature Control Units (Pg. 63)
u Thermo Scientific HyPerforma Smartainer and jacketed Smartainer

system components
uSmartainer/jacketed Smartainer wTray assembly (optional)
Stainless steel specially designed container to Conveniently holds dispense pumps, tube
support the BPC welders, and other items
vBPC hoist (optional) xCart (optional)

Assists in handling and loading the BPC into the Assists in moving the Smartainer and jacketed
Smartainer and jacketed Smartainer systems Smartainer system short distances over smooth
and level surfaces
w
Large volume stainless steel support containers
u Large volume stainless steel outer container systems and accessories
Stainless steel outer containers

Thermo Scientific HyPerforma Smartainer systems
Cat. No. Size Overall Dimensions (W x D x H) Internal Dimensions (W x D x H)
SV50160.01 200 L 79.4 cm x 59.7 cm x 79.1 cm (31¼ in x 23½ in x 31 in) 1/8 73 cm x 53.3 cm x 58.4 cm (28¾ in x 21 in x 23 in)
SV50160.02 500 L 120.7 cm x 78.7 cm x 97.2 cm (47½ in x 31 in x 38¼ in) 114.3 cm x 73.7 cm x 74.5 cm (45 in x 29 in x 291/3 in)
SV50160.03 1,000 L 120.7 cm x 97.8 cm x 135 cm (47½ in x 38½ in x 53 in) 1/8 114.3 cm x 91.4 cm x 114.8 cm (45 in x 36 in x 451/5 in)
SV50160.04 1,500 L 120.7 cm x 97.8 cm x 175.3 cm (47½ in x 38½ in x 69 in) 114.3 cm x 91.4 cm x 152.4 cm (45 in x 36 in x 60 in)
SV50160.05 2,000 L* 120.7 cm x 97.8 cm x 223.5 cm (47½ in x 38½ in x 88 in) 114.3 cm x 91.4 cm x 200.7 cm (45 in x 36 in x 79 in)
*No standard BPC configurations are available. Custom options available.
Note: Larger container system sizes available upon request.
Accessories
Stainless steel hoist (optional) Custom options
Cat. No. Size • 316 stainless steel
SV50161.21 1,000 L • Load cells
SV50161.22 1,500 L • Lid
SV50161.23 2,000 L
• Casters mounted directly on bin
Note: Hoist recommended for all units 1000 L and greater
• Filter bracket
• Pump shelf
• Tubing management
Tray (optional)
Cat. No. Size
SV50161.11 200 L
SV50161.12 500 L
SV50161.13 1,000/1,500 L
Cart (optional)
Cat. No. Size
SV50161.01 200 L
SV50161.02 500 L
SV50161.03 1,000/1,500 L
33
Mixtainer mixing systems
u Thermo Scientific mixtainer mixing systems
Key features
• The Thermo Scientific™ Mixtainer™ System is an integrated, single-use sterile unit for mixing of
cell culture media, and other process liquids such as buffers, reagents and bulk drug precursors
or products
• The Mixtainer system utilizes a sophisticated BPC constructed of CX5-14 Film with a plastic dish
welded into the base of the BPC
• The plastic dish contains a magnetic stir-bar that is held in the proper operating position using an
integrated locking ring
• Available in 50, 100 and 200 L unit volumes
• Standard BPCs available for liquid/liquid- and powder/liquid-mixing
• Supporting hardware optimized for mixing, storage, transport and discharge in a closed system to
help minimize the risk of cross-contamination
Mixtainer BPC Conical drum Magnetic stirring plate

4 ports, top dispense, powder/liquid Top dispense, with clamps IKAMAGTM motor and support required
mixing
Line 1 9.5 mm (3/8 in) Quick Connect Cat. No. Size Cat. No. Area Description
insert with 16.5 cm
SV50517.11 50 L 230 V, 50/60 Hz,
(6.5 in) dip tube SV30097.01 EU
0.5 amp
tube length: 91 cm (36 in)
SV50517.12 100 L
Line 2 6.3 mm (1/4 in) male luer lock
SV30097.02 115 V, 50/60 Hz,
US
tube length: 91 cm (36 in) SV50517.13 200 L 1 amp
Line 3 6.3 mm (1/4 in) female luer lock
tube length: 91 cm (36 in)
Line 4 9.53 mm (3/8 in) Quick Connect

body tube length: 91 cm (36 in)
Stainless steel support Dolly
Dimensions (D x H)
Powder addition port: 38.1 mm (1 /2 in)
1 62 cm x 18 cm (243/8 in x 71/8 in)
tri-clamp port
Cat. No. Size

Cat. No. Area Description
SH30687.04 50 L
Stainless steel
SV30097.03 EU
SH30687.05 100 L support
Cat. No. Size
SV30097.04 Stainless steel
SH30687.06 200 L US SV50109.01 Universal
support
35
u Thermo Scientific mixtainer mixing systems
Mixtainer systems information
Custom BPC options Description
Dip tubes Specific lengths 6.36–12.7 mm (1/4–1/2 in) ID

C-Flex (clear or white)
Tubing type Silicone
PVC
Tubing size Specific lengths of 3.18–19.05 mm (1/4–1/2 in) ID
Luer ID: 3.18–6.35 mm (1/8–1/4 in)
CPC ID: 6.35–19.05 mm (1/4–3/4 in)
Connectors SIP tri-clamp ID: 6.35–19.05 mm (1/4–3/4 in)
Mini tri-clamp ID: 6.35–19.05 mm (1/4–3/4 in)
Hose barb
Injection port ID: 6.35–9.53 mm (1/4–3/8 in)
Others Filling bell ID: 6.35 mm (1/4 in)
Filter capsule: Millipore, Pall, Sartorius, Domnick Hunter
BPC presentation Description
Supplied “flat-packed”
Outer packaging
Two polyethylene outer layers
Description
Product code
Label
Lot number
Expiry date on outer packaging and shipping container
Sterilization Irradiation (25 to 38 kGy) inside outer packaging
Shipping container Durable cardboard carton
Documentation Certificate of Analysis provided
System options Material Supplier information
Thermo Scientific™ Powdertainer™ II 304 stainless steel Saint Patricks of Texas:

powder feed bag: connect a Powder- ordering info—concentric reducer (3 x 1.5 in)
tainer II to the Mixtainer using a concentric www.stpats.com/sanitaryfittings.htm • phone: (512) 989-9727
reducer 7.6 cm (3 in) tri-clamp to 3.8 cm 304 stainless steel RT Process:
(11/2 in) tri-clamp. Ordering information part number 31-14MP-3X1.5-304
for the reducer is shown. www.rtprocess.com • phone: (801) 561-8877
316 stainless steel RT Process:
part number 31-14MP-3X1.5-316
www.rtprocess.com • phone: (801) 561-8877
Polypropylene Saint-Gobain (Sani-Tech):
part number TAD300-150
www.plastics.saint-gobain.com • phone: (800) 543-8823
Kynar material (PVDF) Saint-Gobain (Sani-Tech):
part number KAD300-150
www.plastics.saint-gobain.com • phone: (800) 543-8823
Thermo Scientific HyPerforma DS 300 Single-Use Mixer
u HyPerforma DS 300 Single-Use Mixer
Key features
• The Thermo Scientific™ HyPerforma™ DS 300 Single-Use Mixer (S.U.M.) is a modular

mixing system consisting of a mixing station that mates to plastic drums containing
single-use BPCs or tank liners, offering the user a cost-effective, docking station–style
mixing platform with multiple mixing volume options
• The docking station consists of a stainless steel base with locking casters, an adjustable
handle, a tethered hand-held control device, an electronic vertical lift mechanism with
integrated height indicator, motor mount and motor, three sizes of drive shafts, two
adjustment tools—a spanner and a torque wrench. An adjustable-angle motor head and
drum positioned are available as add-on options
• Top-drain support containers are available in 50, 100, 200 and 300 L sizes; with
bottom-drain drums available in 50, 100 and 200 L sizes
• Top-drain tank liners are available in four sizes from 50 to 300 L
• Tank liners with bottom access ports are available in three sizes from 50 to 200 L
• Closed-top 3D BPCs, with both top and bottom drain access ports, are available in sizes 50,
100 and 200 L
• Optional dollies are available for all drum sizes
37
HyPerforma DS 300 S.U.M. system
1. Motor assembly
2. Horizontal support arm
3. Height indicator and cable
management housing
4. Vertical lift
5. Handheld control device
6. Electrical box
7. Main disconnect switch
8. E-stop reset button
9. Drive shaft (stored)
10. Mounted drive shaft, sleeve
and impeller
11. Plastic drum
12. Liquid drain port
13. Conical insert
Figure 1: S.U.M. DS 300 complete system diagram–front view.
14. Dolly
15. Adjustable handle (in stored position)

16. Reusable bearing hub (open top
applications only)
17. Square torque wrench
18. Spanner wrench
19. Drum positioner (optional)
20. Locking rear casters
Figure 2: S.U.M. DS 300 complete system diagram–back view.
u HyPerforma DS 300 S.U.M.
HyPerforma DS 300 S.U.M. information

DS 300 S.U.M. options
Cat. No. Description
SUMDS0300.9000 DS300 unit standard
SUMDS0300.9001 DS300 unit with drum positioner
SUMDS0300.9002 DS300 unit with adjustable motor head
SUMDS0300.9003 DS300 unit with drum positioner and adjustable motor head
DS 300 S.U.M. drum options

Cat. No. Description Dimensions Bottom drain Volume range
SH30959.01 50 L plastic drum with conical insert 60 x 58 cm (23.5 x 23 in) 10.2 cm (4 in) 10–50 L
SH30959.02 100 L plastic drum with conical insert 60 x 76 cm (23.5 x 29.75 in) 10.2 cm (4 in) 20–100 L
SH30959.03 200 L plastic drum 60 x 114 cm (23.5 x 44.75 in) 10.2 cm (4 in) 40–200 L
SH30959.04 300 L plastic drum 61 x 122 cm (24 x 48 in) N/A 60–300 L
SV50102.02 optional support plate for bottom drain 12.7cm (5 in) diameter split N/A 50–200 L
DS 300 S.U.M. dolly options

Cat. No. Description Material Quantity
SH30958.01 dolly for 50–200 L plastic drum polyethylene 1
SH30958.02 dolly for 50–200 L plastic drum polyethylene 2
SH30958.03 dolly for 300 L plastic drum stainless steel 1
DS 300 S.U.M. tank liner specifications

Impeller material USP class VI, HDPE
Impeller to BPC system/tank liner location 5:1 off center
Number of impeller blades 3
Closed-top BPC system qualified LDPE
Open-top BPC tank liner qualified LDPE
DS 300 S.U.M. irradiated BPC tank liner options
Cat. No. Size Description

CX3-9 Film: 50 L
SH30646.01
CX3-9 Film: 100 L open-top, bottom drain, irradiated

SH30646.02
CX3-9 Film: 200 L

SH30646.03
CX3-9 Film: 300 L open-top, top drain, irradiated

SH30647.06
39
DS 300 S.U.M. non-irradiated BPC tank liner options

Cat. No. Size Description
CX3-9 Film:
SH30399.01 50 L
CX3-9 Film:
SH30399.02 100 L open-top, bottom drain, non-irradiated
CX3-9 Film:
SH30399.03 200 L
DS 300 S.U.M. impeller sleeve options
Cat. No. Drum size Sleeve length Description

SH30749.11 50 L 35.3 cm (13.9 in) impeller, sleeve and connector for 66 cm (26 in) drive shaft
SH30749.12 100 L 53.1 cm (20.9 in) impeller, sleeve and connector for 83.8 cm (33 in) drive shaft
SH30749.13 200 L 77 cm (30.3 in) impeller, sleeve and connector for 108 cm (42.5 in) drive shaft
SH30749.08 300 L 96 cm (37.8 in) impeller, sleeve and connector for 129.5 cm (51 in) drive shaft
DS 300 S.U.M. reusable hub options

Part number Size Description
SV50177.77 50–300 L stainless steel bearing hub
DS 300 S.U.M. irradiated BPC system options
Part number Size Description

Aegis5-14 Film:
SH30946.01 50 L
Aegis5-14 Film: closed-top, bottom drain,

SH30946.02 100 L
irradiated
Aegis5-14 Film:
SH30946.03 200 L
Thermo Scientific HyPerforma DS 300 Single-Use
cell culture
Mixer
u HyPerforma DS 300 S.U.M.
HyPerforma DS 300 S.U.M. information

DS 300 S.U.M. BPC system specifications
Tubing set
Description End treatment
(Inner diameter x outer diameter x length)
polycarbonate Quick Connect 12.7 mm (1/2 in) insert (MPX) and
Bottom drain line 1.27 x 1.91 x 340.6 cm (1/2 in x 3/4 in x 11.17 ft)
cap
polycarbonate Quick Connect 9.5 mm (3/8 in) body (MPC) and
Addition line 1 0.95 x 1.59 x 61 cm (3/8 in x 5/8 in x 2.0 ft)
plug
38.1 mm (11/2 in) Tri-clamp with 19.1 mm (3/4 in) hose barb,
Addition line 2 1.91 x 2.54 x 121.9 cm (3/4 in x 1 in x 4.0 ft)
with gasket SterilEnz II/H
Vent filter 0.64 x 1.27 x 10.1 cm (1/4 in x 1/2 in x 0.33 ft) hydrophobic vent with Emflon II, HB
polycarbonate Quick Connect 12.7 mm (1/2 in) body (MPX) and
1.27 x 1.91 x 91 cm (1/2 in x 3/4 in x 3 ft ) plug
Recirculation/sample
1.27 x 1.91 x 30.5 cm (1/2 in x 3/4 in x 1 ft)
line
1.27 x 1.91 x 61 cm (1/2 in x 3/4 in x 2 ft) polycarbonate Quick Connect 12.7 mm (1/2 in) insert (MPX) and
cap
DS 300 S.U.M. general specifications

Power 108–240 VAC, 50–60 Hz
Input amperage 15 amp
Operating temperature range ambient to 40°C
Motor speed 30 to 350 RPM
Foot print (L x W) 86.4 x 76.2 cm (34 x 30 in)
Height (lowest to tallest 122.4–162.3 cm (48.2–63.9 in)
point)
Weight 180 kg (398 lb)
Control box built to IP65 standards
Flow type radial/axial
Hardware material 304 stainless steel
41
Typical open-top mixing with standard impeller sizes

Minimum Motor angle
Turndown working Minimum Maximum Left to right Front to back (fixed or Motor
Drum size Lift height*
ratio liquid hold up liquid fill offset* position* adjustable motor RPM
volume head models)
2.5 cm
50 L 5:1 10 L 100 mL 50 L 0 0.48cm (3/16 in) 10° 350
(1 in)
5.1 cm
100 L 5:1 20 L 100 mL 100 L 0 2.2cm (7/8 in) 10° 350
(2 in)
5.4 cm 30.64 cm
200 L 5:1 40 L 100 mL 200 L 0 10° 350
(21/8 in) (12 1/16 in)
1.3 cm 6.4 cm 39.98 cm
300 L 4:1† 79 L 500 mL 300 L 10° 350
(1/2 in) left (21/2 in) (15.74 in)
*All measurements listed in the above table are ± 0.16 cm ( /16 in)
1
†For the 300 L drum, a 5:1 turndown ratio can be achieved using the adjustable angle motor head
Minimum open-top mixing for adjustable motor head S.U.M.s with standard impeller sizes
Minimum
Motor angle
Turndown working Minimum Maximum Left to right Front to back Motor
Drum size Lift height* (adjustable motor
ratio liquid hold up liquid fill offset* position* RPM†
head models only)
volume
2.5 cm 1.3 cm
50 L 5.4:1 9.2 L 100 mL 50 L 0 5° 180
(1 in) left (1/2 in)
2.5 cm 1.3 cm
100 L 10:1 9.8 L 100 mL 100 L 0 5° 205
(1 in) left (1/2 in)
2.5 cm 1.3 cm 2.2 cm
100 L 6:1 16.7 L 100 mL 100 L 5° 140
(1 in) left (1/2 in) (7/8 in)
1.9 cm 3.2 cm 28.1 cm
200 L 10:1 20 L 100 mL 200 L 5° 150
(3/4 in) left (11/4 in) (11 1/16 in)
1.9 cm 3.2 cm 28.3 cm
200 L 8.8:1 22.5 L 100 mL 200 L 5° 140
(3/4 in) left (11/4 in) (11 1/8 in)
N/A due to flat 38.71 cm
300 L 5:1 61 L 500 mL 300 L N/A 5° 164
bottom (15.24 in)
N/A due to flat 38.71 cm
300 L 5:1 65 L 500 mL 300 L N/A 5° 124
bottom (15.24 in)
*All measurements listed in the above table are ± 0.16 cm (1/16 in)
†All values listed are ± 5 RPM
HyPerforma Single-Use
cell culture
Mixer
u HyPerforma Single-Use Mixer
The Thermo Scientific™ HyPerforma™ S.U.M. has been designed

using a proven engineered mixer platform design for upstream and
downsteam mixing applications. The HyPerforma S.U.M. offers a
more ergonomic and efficient user experience. The complete line of
HyPerforma S.U.M.s includes 50, 100, 250, 500, 1,000, and 2,000 L sizes
that helps ensure consistent scalability while providing a number of
application usages from powder media and buffer hydration to advanced
downstream havest and intermediate pooling steps.
Key features
The Hyperforma S.U.M. is a fully functional single-use mixing vessel including single-use sterile
contact surfaces for mixing, venting and temperature sensing. Additional ports are included to allow
choice of aseptic connections for filling, emptying and sampling.
• Unique proprietary mixing system that provides conventional overhead mixing while
maintaining sterility and integrity of the S.U.B. BPC
• Minimized vessel footprint and reduced hold-up volumes
• Simplified bag loading with a vertical access door (available on 500 L, 1,000 L sizes and
on 2,000 L S.U.M.)
• Cable management system provides greater organization for electrical cables, tubing and
line sets while making the unit easier to clean.
• Dedicated adjustable tool holder keeps necessary tools available for convenience and ease
• Mixer vessel is offered with electrical non-jacketed (no heat transfer) or water jacket
(vessel side and bottom) for integration with external temperature control unit.
• Offered with choice of AC motor or DC motor with precise agitation control. Brushless DC
motor includes encoder feedback to help improve RPM accuracy and is GFCI-compatible.
(Electrical box not available with DC motors).
• Three load cells weighing systems enable accurate batch weight monitoring.
• Option to use probes for temperature, pH, conductivity and pressure monitoring.
• Electrical box with communication cable that provides output signals to customer plant
controller
• S.U.B. BPCs offered in CX5-14 and Aegis5-14 Films
Applications Associated products
• Powder media hydration • 2D BPC Systems (Pg. 19)

• Liquid media concentrate dilution • 3D BPC Systems (Pg. 23)
• Harvest hold tank • Temperature Control Units (Pg. 63)
• Viral inactivation • Powdertainer BPC System (Pg. 50)
• Downstream intermediate pooling • Services and Support (Pg. 69)
• Final product hold and additive suspension
43
HyPerforma Single-Use Mixer
HyPerforma S.U.M. specifications

Specifications 50 L 100 L 200 L 500 L 1,000 L 2,000 L
Maximum liquid working volume 50 L 100 L 200 L 500 L 1,000 L 2,000 L
Minimum liquid working volume 10 L 20 L 40 L 100 L 200 L 400 L
Fluid geometry at working volume 1.5:1 1.5:1 1.5:1 1.5:1 1.1:1 1:1
(height/diameter) ratio
Overall reactor geometry 1.9:1 1.5:1 1.95:1 1.7:1 1.2:1 1.2:1

Impeller (quantity x blade count) 1X3 1X3 1X3 1X3 1X3 1X3
Mixing rate range 30–350 RPM 30–350 RPM 30–350 RPM 30–350 RPM 30–350 RPM 30–350 RPM
Tank overall W x L x H 94.2 x 84.3 x 94.8 x 87.3 x 94.8 x 87.3 x 122.5 x 130.2 148.3 x 155.8 172.2 x 159.6 x
(including cable management tree 198.6 cm (37.1 x 175.3 cm (31.8 x 175.3 cm (37.3 x x 251.1 cm (48.23 x 201 cm (58.4 x 224.8 cm (67.8 in
and electrical box) 33.2 x 78.2 in) 36.1 x 60.35 in) 34.4 x 69 in) x 51.25 x 98.9 in) 61.33 x 79.10 in) 62.83 x 88.52 in)
Standard hardware part numbers

Cat. No. Description Size Cat. No. Description Size
SUM0050.9001 Non-jacketed, DC motor, without load cell 50 L SUM1000.9001 Non-jacketed, DC motor 1,000 L
SUM0050.9002 Jacketed, DC motor, without load cell 50 L SUM1000.9002 Water-jacketed, DC motor 1,000 L
SUM0050.9003 Non-jacketed, 120VAC, AC motor, without load cell 50 L SUM1000.9003 Non-jacketed, 120VAC, AC motor 1,000 L
SUM0050.9004 Jacketed, 120VAC, AC motor, without load cell 50 L SUM1000.9004 Water-jacketed, 120VAC, AC motor 1,000 L
SUM0100.9001 Non-jacketed, DC motor, without load cell 100 L SUM2000.9003 Non-jacketed, 120VAC, AC motor 2,000 L
SUM0100.9002 Jacketed, DC motor, without load cell 100 L SUM2000.9004 Water-jacketed, 120VAC, AC motor 2,000 L
SUM0100.9005 Non-jacketed, 240VAC, AC motor, without load cell 100 L
SUM0100.9006 Jacketed, 240VAC, AC motor, without load cell 100 L
SUM0250.9001 Non-jacketed, DC motor, without load cell 200 L
SUM0250.9002 Jacketed, DC motor, without load cell 200 L
SUM0500.9001 Non-jacketed, DC motor 500 L
SUM0500.9002 Water-jacketed, DC motor 500 L
SUM0500.9003 Non-jacketed, 120VAC, AC motor 500 L
SUM0500.9004 Water-jacketed, 120VAC, AC motor 500 L
SUM0500.9005 Non-jacketed, 240VAC, AC motor 500 L
SUM0500.9006 Water-jacketed, 240VAC, AC motor 500 L
Note: Non-jacketed models do not provide heat transfer.
HyPerforma S.U.M. docking
cell culture
station
u HyPerforma S.U.M. docking station option
The S.U.M. is based on the same concept as the Thermo Scientific

S.U.B. with a single-use BPC with an integrated impeller and a hardware
system providing support, mobility, mixing motor and controller. In con-
trast to the other options in the S.U.M. product family the docking station
system has the mixing motor mounted on a separate docking station
rather than on the tank itself. This allows the mixing motor to link with a
range of separate tanks. Easy-to-use motor height and angle adjustment
combined with a secure mechanism to locate and lock the docking sta-
tion and tank in place helps make the changeover of tanks simple and
rapid.
Key features
The stainless steel tank options include:
• Maximum volumes of 200, 500 and 1,000 L

• Mixer vessel is offered with a non-jacketed (no heat transfer) or water jacket for integration
with external temperature control unit
• Three load cells weighing systems to enable accurate batch weight monitoring
• Option to use probes for temperature, pH, conductivity and pressure monitoring
• Electrical box with communication cable that provides output signals to customer plant
controller
HyPerforma S.U.M. docking station specifications

Item Docking station frame
Width 81.3 cm (34 in)
Depth 113.8 cm (44.8 in)
Height 61.9 cm (61.9 in)
Weight 231 kg (509 lb)
Electrical power supply requirement 120 or 240 VAC, single,
(voltage, phase, amp) 15 amp, non-GFCI circuit
Top stair height 46.2 cm (18.2 in)
Programmable VFD, remote panel interface Standard
Mixer motor drive (voltage, phase, amp) 208 VAC, three, 15 amp
Motor power rating 0.5 hp (372.8 W)
Motor torque rating 80 lbf-in (9.04 Nm)
Gear reduction 5:1
45
HyPerforma S.U.M. docking tank specifications

Item 200 L 500 L 1,000 L
Rated liquid working volume 200 L 500 L 1,000 L
Minimum liquid working volume 40 L 100 L 200 L
BPC chamber diameter 50.8 cm (20 in) 75.6 cm (29.75 in) 105.4 cm (41.5 in)
BPC chamber shoulder height 123.4 cm (48.6 in) 146 cm (57.5 in) 157.2 cm (61.9 in)
Liquid height at rated working volume 99 cm (39 in) 111.8 cm (44 in) 115.6 cm (45.5 in)
Overall geometry (height/diameter ratio) 1.95:1 1.5:1 1.1:1
Fluid geometry at working volume (height/diameter) ratio 2.2:1 1.7:1 1.2:1
Impeller (quantity x blade count) 1x3 1x3 1x3
Impeller diameter 20 cm (7.88 in) 20 cm (7.88 in) 20 cm (7.88 in)
Hold-up volume (typical) <50 mL <100 mL <300 mL
Skid width 64.8 cm (25.5 in) 77 cm (30.4 in) 126 cm (49.6 in)
Skid depth 73.7 cm (29 in) 95 cm (37.4 in) 125.2 cm (49.3 in)
Skid height 147.8 cm (58.2 in) 165.3 cm (65.1 in) 161 cm (63.4 in)
Dry skid weight (mass) non-heated tank 201 kg (443 lb) 352 kg (776 lb) 399 kg (880 lb)
Dry skid weight (mass) jacketed tank 211 kg (465 lb) 364 kg (802 lb) 414 kg (913 lb)
Wet skid weight—rated working volume (mass)
401 kg (884 lb) 852 kg (1,878 lb) 1,399 kg (3,084 lb)
non-heated tank
Wet skid weight—rated working volume (mass)
411 kg (906 lb) 864 kg (1,905 lb) 1,414 kg (3,117 lb)
jacketed tank
Minimum ceiling height recommended 305 cm (10 ft) 335 cm (11 ft) 335 cm (11 ft)
58.4 x 55.8 cm 68.6 x 60.9 cm 96.5 x 96.5 in
Minimum required floor scale platform dimensions
(23 x 22 in) (27 x 24 in) (38 x 38 in)
HyPerforma S.U.M. docking station ordering information

Cat. No. Description
SUM0000.XXXX - ("X" is defined at time of quote) Single-Use Mixer (S.U.M.) docking station system (120 VAC)
SUM0000.XXXX - ("X" is defined at time of quote) Single-Use Mixer (S.U.M.) docking station system (240 VAC)
SUM0200.XXXX - ("X" is defined at time of quote) Single-Use Mixer (S.U.M.) docking station tank, non-jacketed, feet
SUM0200.XXXX - ("X" is defined at time of quote) Single-Use Mixer (S.U.M.) docking station tank, non-jacketed, casters
SUM0200.XXXX - ("X" is defined at time of quote) Single-Use Mixer (S.U.M.) docking station tank, jacketed, feet
SUM0200.XXXX - ("X" is defined at time of quote) Single-Use Mixer (S.U.M.) docking station tank, jacketed, casters
Hyperforma S.U.M. standard BPCs

Probe Probe Film
Description Film type Size Cat. No. Description Size Cat. No.
ports ports type
Standard powder-liquid BPC 0 CX5-14 50 L SH30768.01 Standard, open-top liner 0 CX3-9 50 L SH30762.04
Standard powder-liquid BPC 0 Aegis5-14 50 L SH30973.01 Standard, open-top liner 0 CX3-9 100 L SH30762.06
Standard liquid-liquid BPC 0 CX5-14 50 L SH30767.01 Standard, open-top liner 0 CX3-9 200 L SH30762.01
Standard liquid-liquid BPC 0 Aegis5-14 50 L SH30983.01 Standard, open-top liner 0 CX3-9 500 L SH30762.02
Standard powder-liquid BPC 3 CX5-14 50 L SH31055.02 Standard, open-top liner 0 CX3-9 1,000 L SH30762.03
Standard powder-liquid BPC 3 Aegis5-14 50 L SH31051.02 Standard, open-top liner 0 CX3-9 2,000 L SH30762.05
Standard liquid-liquid BPC 3 CX5-14 50 L SH31055.04
Standard liquid-liquid BPC 3 Aegis5-14 50 L SH31051.01 Description Size Cat. No.
Standard powder-liquid BPC 0 CX5-14 100 L SH31046.01 Impeller sleeve for open-top mixing 50 L SH30749.06
Standard powder-liquid BPC 0 Aegis5-14 100 L SH31052.01 Impeller sleeve for open-top mixing 100 L SH30749.14
Impeller sleeve for open-top mixing 200 L SH30749.08
Standard liquid-liquid BPC 0 Aegis5-14 100 L SH31052.03
Impeller sleeve for open-top mixing 500 L SH30749.10
Standard powder-liquid BPC 3 CX5-14 100 L SH31046.02
Impeller sleeve for open-top mixing 1,000 L SH30749.10
Standard powder-liquid BPC 3 Aegis5-14 100 L SH31052.02
Impeller sleeve for open-top mixing 2,000 L SH30772.01
Standard powder-liquid BPC 0 CX5-14 1,000 L SH30752.02
Standard powder-liquid BPC 0 Aegis5-14 1,000 L SH30973.04
Standard liquid-liquid BPC 0 CX5-14 1,000 L SH30755.01
Standard liquid-liquid BPC 0 Aegis5-14 1,000 L SH30983.04
47
HyPerforma S.U.M. accessories

Description 50 L 100 L 200 L 500 L 1,000 L 2,000 L
Cable management tree SV50992.01 SV50992.02 SV50992.02 SV50992.03 SV50992.03 SV50992.03
Load cell with summation box,
SV50988.01 SV50988.02 SV50988.02 SV50988.03 SV50177D.04 SV50177.173
without display
Autoclave tray SV50177.01 SV50177.01 SV50177.01 SV50177.01 SV50177.01 SV50177.01
Bioreactor probe assembly
SH30720.01 SH30720.01 SH30720.01 SH30720.01 SH30720.01 SH30720.01
(non-sterile for use in autoclave)
Heavy-duty tubing clamp (each) SV20664.01 SV20664.01 SV20664.01 SV20664.01 SV20664.01 SV20664.01
Heavy-duty tubing clamps
SV20664.03 SV20664.03 SV20664.03 SV20664.03 SV20664.03 SV20664.03
(10 per pack)
Sterile sampling manifold with luer
SH30845.01 SH30845.01 SH30845.01 SH30845.01 SH30845.01 SH30845.01
lock (each)
Sterile sampling manifold with luer
SH30845.02 SH30845.02 SH30845.02 SH30845.02 SH30845.02 SH30845.02
lock (10 per pack)
Temperature/sample port SV20750.01 SV20750.01 SV20750.01 SV20750.01 SV20750.01 SV20750.01
Reusable hub assembly with
N/A N/A N/A N/A N/A SV50177.78
Tri-clamp
Reusable hub assembly with quick
SV50177.77 SV50177.77 SV50177.77 SV50177.77 SV50177.77 N/A
connect
HyPerforma Single-Use
cell culture
Mixer
S.U.M MTO Configuration Table: This table describes the part number and configurable (make-to-order; MTO) options for the S.U.M. product line.
EX: SUM0500.AAA0000 S.U.M. 0500 A A A A 0 0 0 0 0

Single-Use Mixer
Vessel size
0500 = 500 L
1. Vessel type/material
A = non-jacketed (304 SST) (standard) tank and cart are 304 SST
B = jacketed (304 SST) (standard) tank and cart are 304 SST
C = non-jacketed (316 SST) tank and cart are 316 SST
D = jacketed (316 SST) tank and cart are 316 SST
2. Motor/electrical enclosure: L/H = electrical box mounted on left and cable management tree on right side of
tank. (See DWG) R/H= electrical box mounted on right and cable management Tree on left side of tank. (See
DWG)
A = DC motor/no electrical enclosure
B = AC motor/no electrical enclosure
C = AC motor/120VAC electrical enclosure - R/H configuration (standard)
D = AC motor/240VAC electrical enclosure - R/H configuration (standard)
E = AC motor/120VAC electrical enclosure - L/H configuration (standard)
F = AC motor/240VAC electrical enclosure - L/H configuration (standard)
3. Drive shaft
A = 1 piece drive shaft (SST)
B = 2 piece drive shaft (SST)
4. RTD
0 = no RTD
A = single RTD, PT100, 60 inch Bulgin connector (standard)
B = duel RTD, PT100, 120 inch DB9 connector x 120 inch open lead wires
C = duel RTD, PT100, 120 inch Bulgin x 120 inch Bulgin connectors
D = duel RTD, PT100, 120 inch DB9 x 60 inch bulgin connectors
E = duel RTD, PT100, 120 inch Bulgin x 120 inch LEMO connectors
F = single RTD, PT100, 120 inch Bulgin connector
G = duel RTD, PT100, 120 inch DB9 x 120 Bulgin connectors
H = single RTD, PT100, 60 inch LEMO connector
5. Load cells and display
0 = no load cells
A = load cells, no display
B = load cells, wall mount display, analog output communication
C = load cells, wall mount display, Allen-Bradley communication
D = load cells, wall mount display, device net communication
E = load cells, wall mount display, Modbus communication
F = load cells, wall mount display, Profibus communication
6. Powder arm assembly
0 = no powder arm assembly
A = powder arm assembly
7. Cable management tree: L/H = cable management tree mounted on left and cable management tree on right
side of tank. (See DWG) R/H= cable management tree mounted on right and cable management Tree on left side
of tank. (See DWG)
0 = no cable management tree
A = cable management tree (L/H configuration)
B = cable management tree (R/H configuration)
8. Communication cable
0 = no communication cable
A = communication cable
9. Monitoring device
0 = no monitoring device
A = pH monitoring device, single channel with display
B = pH monitoring device, duel channel with display
C = pH conductivity monitoring device with display
49
Powdertainer BPC systems
u Powder Handling BPCs
Key features
• Specifically designed for powder containment and discharge applications

• Employ a closed system for dust containment and to help minimize cross-contamination
risk
• Three-inch Tri-clamp port designed for secure connection to, and easy integration with
existing formulation vessels
• Suspensor handle for support during discharge
• Neck clamp to retain powder prior to discharge
• Two models including one with a wash-down line to remove residual powder enabling
maximal recovery
• Three sizes for process flexibility: 1 kg, 5 kg and 25 kg
• Constructed from Thermo Scientific CX3-9 and Aegis5-14 Films
• Stainless steel filling stand to facilitate powder discharge process
Applications
• Storage and delivery of Thermo Scientific powder culture media and buffers
• Storage and dispensing of pre-weighed chemicals or other process powders
• CX3-9 Film Fact Sheet

• Powdertainer Data Sheet
Associated products
• HyPerforma S.U.M. (Pg.43)
Handle
For support Tube with CPC connector
during emptying For washdown to remove any
powder residue
Clamp 3-inch tri-clamp connection

To retain powder prior For secure connection to
to emptying formulation vessel
Powdertainer BPC systems
Powdertainer II Filling stand

2 ports 2 ports
Powder
Handling BPCs
Wash down N/A Wash down MPX body, polycarbonate
line line Tubing: length:
20.3 cm (8 in)
ID x OD:12.7 mm x 19.1 mm
(½ in x ¾ in), silicone
Powder port 7.6 cm (3 in) tri-clamp Powder port 7.6 cm (3 in) tri-clamp
with integrated seal with integrated seal
Cat. No. Size Dimensions (L x W) Cat. No. Size Dimensions (L x W) Cat. No. Dimensions (L x W x H)
SV50143.01
CX5-14 Film: 42.7 cm x 31.8 cm CX5-14 Film: 42.7 cm x 31.8 cm 91.4 cm x 91.4 cm x 134.6 cm
1 kg 1 kg (stainless steel,
SH30864.01 (16.8 in x 12.5 in) SH30737.01 (16.8 in x 12.5 in) with casters) (36 in x 36 in x 53 in)
No. per case: 45 No. per case: 20 SV50143.02

91.4 cm x 91.4 cm x 114.3 cm
(stainless steel,
w/o casters) (36 in x 36 in x 45 in)
CX5-14 Film:
5 kg
56.1 cm x 36.3 cm CX5-14 Film: 56.1 cm x 36.3 cm
SH30864.01 SH30737.02 5 kg
(22.1 in x 14.3 in) (22.1 in x 14.3 in)
CX5-14 Film: 25 83.3 cm x 59.2 cm CX5-14 Film: 25 83.3 cm x 59.2 cm

SH30864.01 kg (32.8 in x 23.3 in) SH30737.03 kg (32.8 in x 23.3 in)
51
HyPerforma Single-Use Bioreactors
cell culture
u HyPerforma Single-Use Bioreactors
New HyPerforma™ S.U.B. and BPC are designed using traditional

stainless steel bioreactor principles to ensure optimal cell culture
performance. The complete line of HyPerforma S.U.B.s includes 50, 100,
250, 500, 1,000 and 2,000 L sizes that ensures consistent scalability from
pilot-scale studies, preclinical to commercial production.
Key features
• The S.U.B. is a fully functional bioreactor vessel including single-use

sterile contact surfaces for mixing, venting, sparging and temperature sensing. Additional
ports are included to allow choice of aseptic connections for filling, emptying and sampling
• Unique proprietary mixing system that enables conventional overhead mixing while
maintaining sterility and integrity of the S.U.B. BPC
• Simplified bag loading with a vertical access door (available on 500 and 1,000 L sizes;
pneumatic hoist provided on 2,000 L S.U.B.)
• Universal filter bracket easily adjusts to various heights and positions on the vessel and
facilitates single or dual filters of either 6-inch and/or 10-inch filters.
• Cable management system offers greater organization for electrical cables, tubing and line
sets while making the unit easier to clean
• Dedicated adjustable tool holder keeps necessary tools available for convenience and ease

• Bioreactor vessel is offered with electrical heating blanket or water jacket (vessel side and
bottom) for integration with external temperature control unit.
• Offered with choice of AC motor or DC motor with precise agitation control. Brushless DC
motor includes encoder feedback for improved RPM accuracy and is GFCI-compatible
• Option to choose Bioreactor vessel as open architecture system and integrate with existing
or choice of controllers OR complete turnkey system with a choice of dedicated controls
from Finesse™ or Applikon™. These systems work on PC, Delta V™, Allen-Bradley or
Siemens™ software platforms
• Choice of sparging in combination of Drilled Hole Sparger (DHS) with Frit or Frit with open
pipe sparger to facilitate efficient mass transfer and CO2 stripping
• Option to use electrochemical or single-use sensors for pH, DO and Pressure monitoring
• Possibility to add condenser for 1,000 L and 2,000 L S.U.B.s depending on process need
• S.U.B. BPCs offered in CX5-14 and Aegis5-14 Films
Applications Associated products
• Batch, fed batch and perfusion cultures • 2D BPC Systems (Pg. 19)
• Suspension and microcarrier cultures • 3D BPC Systems (Pg. 23)
• Poven for various celli lines like CHO,

Sp2/0 hybridoma, NS0, PER.C6, HEK293,
Vero, MDCK, etc.
• Bioreactor Control Solutions (Pg. 65)
• Harvestainer Microcarrier
Separation BPC and Systems (Pg. 26)
• Services and Support (Pg. 69)
HyPerforma S.U.B. specifications

Liquid working volume 50 L 100 L 250 L 500 L 1,000 L 2,000 L
Minimum liquid working
25 L 50 L 125 L 250 L 500 L 1,000 L
volume
Total reactor volume
65.5 L 120 L 316 L 660 L 1,320 L 2,575 L
(not a working volume)
Fluid geometry at
working volume (height/ 1.5 1.5 1.5 1.5 1.5 1.5
diameter) ratio
Overall reactor
geometry (height/ 1.9 1.9 1.9 1.9 1.9 1.9
diameter) ratio
Impeller (quantity x
1x3 1x3 1x3 1x3 1x3 1x3
blade count)
Mixing rate range 30–200 RPM 30–200 RPM 30–150 RPM 30–150 RPM 20–110 RPM 20–75 RPM
Tank overall 94.2 x 84.3 x 98.5 x 91.2 x 125.2 x 124.4 143.8 x 139.2 x 179.7 x 171.4
112.8 x 102.2 x 215.5
(including cable 198.6 cm 201.5 cm x 251.1 cm 284 cm x 355.5 cm
cm (44.4 x 40.25 x
management tree) (37.1 x 33.2 x (38.8 x 35.9 x (49.3 x 47.8 x (56.6 x 54.8 x (70.5 x 67.5 x
84.4 in)
width x length x height 78.2 in) 79.3 in) 98.9 in) 111.8 in) 140 in)
140.4 kg 196.40 kg 234.3 kg 328.04 kg 589.67 kg
Resistive tank weight
(309.5 lb) (433 lb) (516.5 lb) (724 lb) (1,300 lb)
dry/wet N/A
190.4 kg 296.20 kg 484.3 kg 828.26 1,589 kg
(at full working volume)
(419.8 lb) (653 lb) (1,067.7 lb) (1,826 lb) (3,505 lb)
141.2 kg 219.09 kg 248.9 kg 362.42 kg 657.71 kg 816.47 kg
Jacketed tank weight
(311.3 lb) (483 lb) (548.7 lb) (799 lb) (1450 lb) (1,800 lb)
dry/wet
191.2 kg 318.88 kg 498.9 kg 862.28 kg 1,637.5 kg 2,812 kg
(at full working volume)
(421.5 lb) (703 lb) (1,099.9 lb) (1,901lb) (3,610 lb) (6,200 lb)
53
u HyPerforma Single-Use Bioreactors
HyPerforma S.U.B. BioProcess Containers in CX5-14 Film
Size Cat. No. (open pipe & Cat. No. (drilled hole &
porous frit sparger) porous frit sparger)
50 L SH30774.01 SH30985.01
100 L SH30774.02 SH30985.02
250 L SH30774.03 SH30985.03
500 L SH30774.04 SH30985.04
1,000 L SH30774.05 SH30985.05
2,000 L SH30774.07* SH30985.07*
2,000 L SH30774.08† SH30985.08†
*With condenser †Without condenser
HyPerforma S.U.B. BioProcess Containers in Aegis5-14 Film
Size Cat. No. (open pipe & Cat. No. (drilled hole &
porous frit sparger) porous frit sparger)
50 L SH30972.01 SH30999.01
100 L SH30972.02 SH30999.02
250 L SH30972.03 SH30999.03
500 L SH30972.04 SH30999.04
1,000 L SH30972.05 SH30999.05
2,000 L SH30972.07* SH30999.07*
2,000 L SH30972.08** SH30999.08**
* With condenser †Without condenser
cell culture
HyPerforma S.U.B. accessories
Cable management tree SV50992.01 SV50992.02 SV50992.02 SV50992.03 SV50992.04 N/A
Load cell with summation

SV50988.01 SV50988.02 SV50988.02 SV50988.03 N/A N/A
box, without display
Autoclave tray SV50177.01 SV50177.01 SV50177.01 SV50177.01 SV50177.01 SV50177.01
Bioreactor probe assembly

(non-sterile for use in SH30720.01 SH30720.01 SH30720.01 SH30720.01 SH30720.01 SH30720.01
autoclave)
Sparger line support SV50177B.19 SV50177B.19 SV50177B.19 SV50177B.14 SV50177B.14 SV50177.65

SV20664.01 SV20664.01 SV20664.01 SV20664.01 SV20664.01 SV20664.01
(single pack)

SV20664.03 SV20664.03 SV20664.03 SV20664.03 SV20664.03 SV20664.03
(10 per pack)
Sterile sampling manifold

SH30845.01 SH30845.01 SH30845.01 SH30845.01 SH30845.01 SH30845.01
with luer lock (single pack)
Sterile sampling manifold

SH30845.02 SH30845.02 SH30845.02 SH30845.02 SH30845.02 SH30845.02
with luer lock (10 per pack)
S.U.B. temperature/sample
SV20750.01 SV20750.01 SV20750.01 SV20750.01 SV20750.01 SV20750.01
port
Complete condenser
system (120 V) including
cart, chill plate and N/A N/A N/A N/A N/A SV50232.01
mounting post with filter
brackets, TCU, and pump
Complete condenser
system (240 V) including
cart, chill plate and N/A N/A N/A N/A N/A SV50232.02
mounting post with filter
brackets, TCU, and pump
Mobile stairs N/A N/A N/A N/A N/A SV50935.01
55
HyPerforma S.U.B. MTO configuration table
S.U.B. MTO Configuration Table: This table describes the part number and configurable (make-to-order; MTO) options for the S.U.B. product line.
EX: SUB0500.AAA0000 S.U.B. 0500 A A A A 0 0

Single-Use Bioreactor
Vessel Size
0050 = 50 L
0100 = 100 L
0250 = 250 L
0500 = 500 L
1000 = 1,000 L
2000 = 2,000 L
Heater type/material
A = resistive (304 SST) (standard) tank and cart are 304 SST
B = jacketed (304 SST) (standard) tank and cart are 304 SST
C = resistive (316 SST) tank and cart are 316 SST
D = jacketed (316 SST) tank and cart are 316 SST
Motor/electrical type
A = DC motor/no electrical enclosure
B = AC motor/no electrical enclosure
C = AC motor/120VAC electrical enclosure - R/H configuration (standard) (resistive heater) (50–250 L only)
D = AC motor/240VAC electrical enclosure - R/H configuration (standard) (resistive heater)
E = AC motor/120VAC electrical enclosure - L/H configuration (resistive heater) (50–250 L only)
F = AC motor/240VAC electrical enclosure - L/H configuration (resistive heater)
G = AC motor/120VAC electrical enclosure - R/H configuration (standard) (jacketed heater) (50–250 L only)
H = AC motor/240VAC electrical enclosure - R/H configuration (standard) (jacketed heater)
J = AC motor/120VAC electrical enclosure - L/H configuration (jacketed heater) (50–250 L only)
K = AC motor/240VAC electrical enclosure - L/H configuration (jacketed heater)
Drive shaft
A = standard 1 piece drive shaft (aluminum) (50 L only)
B = standard 1 piece drive shaft or segmented pieces (100 L only)
C = standard 1 piece drive shaft (aluminum) (250 L only)
D = 1 piece drive shaft (SST) (250 L only)
E = 2 piece drive shaft (SST) (250 L only)
F = standard 2 piece drive shaft (SST) (500 L only)
G = 3 piece segmented drive shaft (1,000 L only)
H = 4 piece segmented drive shaft (1,000 L only)
J = 4 piece segmented drive shaft (carbon fiber)
RTD
A = single RTD, 60 inch Bulgin connector
B = duel RTD, PT100, 3 wire each, 120 inch DB9 connector on one lead and exposed wires on the other lead
C = duel RTD, PT100, 120 inch 2x Bulgin connectors
D = duel RTD element, PT100, 3 wire each, 120 inch, DB9 and quick connect
E = duel RTD, PT100, 120 inch Bulgin and LEMO connectors
F = single RTD, 120 inch Bulgin connector
Load cells and display
0 = no load cells
A = load cells, no display (50-500 L)
B = load cells, wall mount display, analog output communication (50–500 L, 2000 L)
C = load cells, wall mount display, Allen-Bradley communication (50–500 L, 2000 L)
D = load cells, wall mount display, device net communication (50–500 L, 2000 L)
E = load cells, wall mount display, Modbus communication (50–500 L, 2000 L)
F = load cells, wall mount display, Profibus communication (50–500 L, 2000 L)
G = load cells, panel mount display, analog output communication (1,000 L)
H = load cells, panel mount display, Allen-Bradley communication (1,000 L)
J = load cells, panel mount display, device net communication (1,000 L)
K = load cells, panel mount display, Modbus communication (1,000 L)
L = load cells, panel mount display, Profibus communication (1,000 L)
Cable management tree
HyPerforma Single-Use Fermentors
cell culture
u HyPerforma Single-Use Fermentor
The Thermo Scientific™ HyPerforma™ S.U.F. is designed to

provide you with enhanced functionality, ease of use, and
efficiency. The complete HyPerforma S.U.F. system consists of a
fermentor tank and Thermo Scientific™ HyPerforma™ S.U.F. BPC,
which is available in 30 L and 300 L sizes with a 5:1 turndown ratio.
The HyPerforma S.U.F. maintains traditional stirred-tank fermentation
design principles, including specific height-to-diameter ratios (3:1),
top-driven impeller location that deliver optimum cell viability, and
performance and scalability from process development through
production.
Key features
• The S.U.F. is a fully functional fermentor vessel including single-use sterile contact surfaces
for mixing, venting, sparging and temperature sensing. Additional ports are included to allow
choice of aseptic connections for filling, emptying and sampling
• Unique proprietary mixing system enables conventional overhead mixing while

maintaining sterility and integrity of the S.U.F. BPC
• Simplified bag loading with a front access door
• Tank includes baffles that enhance mixing
• Side and bottom jacket for effective heat transfer
• Cable management system offers greater organization for electrical cables, tubing and line
sets while making the unit easier to clean

• Dedicated adjustable tool holder keeps necessary tools
• BPC utilizes three Rushton-style impellers and sparger configuration with a proprietary
exhaust management system
• State-of-the-art foam sensor that helps prevent exhaust filter failure
Associated products
• Bioreactor Control Solutions (Pg. 65)
57
Options
• Option to choose Bioreactor vessel as open architecture system and integrate with existing
or choice of controllers OR complete Turnkey system with a choice of dedicated controls
from Finesse or Applikon. These systems work on PC, Delta V, Allen-Bradley or
Siemens software platforms
• Exhaust condenser unit
• Choice of exhaust filters from Meissner or ZenPure™
• Option to use electrochemical or single-use sensors for pH, DO and pressure monitoring
• S.U.F. BPCs offered in CX5-14 and Aegis 5-14 Films
Applications
• Proven for various microbial cultures of Pichia and Bacillus
• Batch and fed-batch cultures
cell culture
HyPerforma S.U.F. specifications
Specifications 30 L 300 L
Liquid working volume 30 L 300 L
Minimum liquid working volume 6L 60 L
Total reactor volume
43 L 435 L
(not a working volume)
Fluid geometry at working volume
~2:1 ~2:1
Overall reactor geometry 3:1 3:1
Impeller (quantity x blade count) 3x6 3x6
Mixing rate range 55–600 RPM 35–375 RPM
Tank overall (including cable
121.8 x 103.2 x 218.5 cm 160.8 x 136.7 x 281 cm
management tree)
(48 x 40.7 x 86 in) (63.3 x 53.8 x 110.6 in)
width x length x height
Jacketed tank weight dry/wet 238 kg (524 lb) 555 kg (1,223 lb)
(at full working volume) 268 kg (590 lb) 855 kg (1,883 lb)
59

HyPerforma
S.U.F.
HyPerforma S.U.F. BioProcess Containers, CX5-14 Film
Description 30 L 300 L
MT single-use pH & DO, foam sensor, Meissner inlet and 1 x 5 inch exhaust filters, condenser SH31019.01
Traditional ports, foam sensor, Meissner inlet and 1 x 5 inch exhaust filters, condenser SH31036.01
Traditional ports, foam sensor, ZenPure inlet and 1 x 3 inch exhaust filters, condenser SH31034.01
Traditional ports, ZenPure inlet and 1 x 5 exhaust filters SH31039.01
Traditional ports, foam sensor, ZenPure inlet and 1 x 10 exhaust filters, condenser SH31030.01
Traditional ports, foam sensor, ZenPure inlet and 2 x 10 exhaust filters, condenser SH31030.02
Traditional ports, ZenPure inlet and 1 x 10 inch exhaust filters SH31030.04
Traditional ports, ZenPure inlet and 2 x 10 inch exhaust filters SH31030.03
HyPerforma S.U.F. BioProcess Containers, Aegis5-14 Film
HyPerforma S.U.F. accessories
Cable management system SV51006.01
Cable management system, left-hand configuration SV51006.02
Cable management system, right-hand configuration SV51006.03
Bottle management system SV50992.10 SV50992.10
Feed bag management hook SV51006.03 SV51006.03
Exhaust filter pinch clamp for 30 L system SV50177E.16
Exhaust filter pinch clamp for 300 L system SV50177E.15
Autoclave tray SV50177.01 SV50177.01
Bioreactor probe assembly (Non-sterile for use in autoclave) SH30720.01 SH30720.01
Heavy-duty tubing clamp (single pack) SV20664.01 SV20664.01
heavy-duty tubing clamp (10 per pack) SV20664.03 Sv20664.03
Sterile sampling manifold with luer lock (single pack) SH30845.01 SH30845.01
Sterile sampling manifold with luer lock (10 per pack) SH30845.02 SH30845.02
S.U.B. temperature/sample port SV20750.01 SV20750.01
Complete condenser system with 120 VAC for 30 L S.U.F unit (TCU for condenser is included) SV51009.02 N/A
Complete condenser system with 240 VAC for 30 L S.U.F unit (TCU for condenser is included) SV51009.03 N/A
Complete condenser system with 120 VAC for 300 L S.U.F unit (TCU for condenser is included) N/A SV51009.01
Thermo Scientific Masterflex™ pump (115 VAC/50 or 60 Hz, or 230 VAC/50 or 60 Hz) for 300 L system N/A SV50241.02
NEMA-rated 120 VAC vent filter heater with a thermocouple–includes preset temperature of 55°C and a Turck™ SV50191.41 SV50191.41
connector
NEMA-rated 240 VAM vent filter heater with a thermocouple–includes preset temperature of 55°C and a Turck SV50191.42 SV50191.42
connector
NEMA-rated 120 VAM vent filter heater with a thermocouple–includes preset temperature of 55°C and a Turck SV50191.35 SV50191.35
connector and a standard IEC C14 plug
NEMA-rated 240 VAC vent filter heater with a thermocouple–includes preset temperature of 55°C and a Turck SV50191.36 SV50191.36
connector and a standard IEC C14 plug
3x load cell with summing box without display SV50988.04 SV50988.03
Mettler-Toledo™ IND331 display, harsh mount style with analog interface (STD), 120 VAC US line cord/plug SV50177.306 SV50177.306
Mettler-Toledo IND331 display, harsh mount style with Allen-Bradley RIO interface, 120 VAC US line cord/plug SV50177.307 SV50177.307
Mettler-Toledo IND331 display, harsh mount style with device net interface, 120 VAC US line cord/plug SV50177.308 SV50177.308
Mettler-Toledo IND331 display, harsh mount style with ethernet/IP and Modbus TCP interface, 120 VAC line chord/ SV50177.309 SV50177.309
plug
Mettler-Toledo IND331 display, harsh mount style with Profibus interface, 120 VAC US line chord/plug SV50177.310 SV50177.310
61
HyPerforma S.U.F. MTO configuration table
S.U.F. MTO Configuration Table: This table describes the part number and configurable (make-to-order; MTO) options for the S.U.F. product line.
EX: SUF0500.AAA0000 S.U.F. 0300 - - - - - - - - - - - -

Single-Use Fermentor
Vessel size
0030 = 30 L
Vessel type/material
A = jacketed (304 SST) (standard)
B = jacketed (316 SST) (standard)
Motor/electrical type
L/H = electrical box mounted on left and cable management tree on right side of tank. (See
DWG) R/H= electrical box mounted on right and cable management Tree on left side of tank.
(See DWG)
A = AC motor/no electrical enclosure

B = AC motor/120VAC electrical enclosure - L/H configuration (standard)
C = AC motor/120VAC electrical enclosure - R/H configuration
D = AC motor/240VAC electrical enclosure - L/H configuration (standard for 300 L)
E = AC motor/240VAC electrical enclosure - R/H configuration
Drive shaft
A = standard 1 piece or segmented drive shaft (vessel specific size)
RTD
0 = no RTD
A = single RTD (standard)
Load cells and display
0 = no load cells
A = load cells, no display
B = load cells, wall mount display, analog output communication
C = load cells, wall mount display, Allen-Bradley communication
D = load cells, wall mount display, device net communication
E = load cells, wall mount display, Modbus communication
F = load cells, wall mount display, Profibus communication
Cable management tree
L/H = electrical box mounted on left and cable management tree on right side of tank. (See
DWG) R/H= electrical box mounted on right and cable management tree on left side of tank.
(See DWG)


Condenser/vent filter heater bracket
A = 3 position vent filter heater bracket with exhaust clamp (default option included in base price)
B = condenser with 3 position vent filter heater bracket (includes TCU and TCU hoses)
Other options
0 = no other options included
Other options
Pinch clamp options
B = pinch clamp for 30 L without condenser
C = pinch clamp for 30 L with condenser
Other options
Other options
Temperature control units
u Thermo Scientific ThermoFlex recirculating chiller
sub-catagory
The environmental conditions of single-use bioreactors, fermentors, and
media mixers need to be closely monitored and controlled in order to
provide optimal growth conditions. One of the most important conditions
is temperature, which must be accurately regulated to ensure reliability
and consistency of your processes.
Key features
• Air- and water-cooled condenser
• Auto-refill
• Flow control with flow readout
Associated products
• Large Volume Stainless Steel Outer Containers (Pg. 32)
ThermoFlex™ chiller specifications ThermoFlex 2,500 chiller ThermoFlex 5,000 chiller ThermoFlex 10,000 chiller ThermoFlex 24,000 chiller
Cooling capacity, 60 Hz* 2,500 W 5,000 W 10,000 W 24,000 W
Cooling capacity, 50 Hz* 2,200 W 4,400 W 8,500 W 21,000 W
Heater capacity 2.8 kW at 230 V 2.8 kW at 230 V 6.1 kW at 460 V 22.5 kW at 460 V
Temperature range –5ºC to 50ºC –5ºC to 50ºC –5ºC to 50ºC –5ºC to 50ºC
Temperature stability ±0.1ºC ±0.1ºC ±0.1ºC ±0.1ºC
Air-cooled condenser Y Y Y Y
Water-cooled condenser Y Y Y Y
Anti-drainback Y Y Y Y
Auto-refill Y Y Y Y
Flow control with flow readout Y Y Y Y
Pump performance, 60 Hz† 3.5 gpm at 60 PSID 10 gpm at 32 PSID 10 gpm at 32 PSID 20 gpm at 60 PSID
Pump performance, 50 Hz† 9.5 lpm at 1.4 bar 37.9 lpm at 1.4 bar 37.9 lpm at 1.4 bar 75.7 lpm at 1.4 bar
Communications Analog and digital Analog and digital Analog and digital Analog and digital
Power options Global voltage Global voltage 50/60 Hz, 3 phase 50/60 Hz, 3 phase
* Capacity at 20ºC.
†Positive displacement and magnetically-coupled centrifugal pump options are available.
63
Temperature control units
ThermoFlex chiller specifications TCU hose kit TCU ball valve
Cat. No. Description Cat. No. Description Cat. No. Description
SV50239.27 ThermoFlex 2,500 temperature control SV50239.31 TCU hose kit 3/4 inch ID, 10 ft, SV50239.34 TCU ball valve kit right angle,
unit (TCU), 2.5 kW cooling 2.8 W heating, 1 1/2 inch tri-clamp 1/2 inch NPT, ball valve
–5–50°C, air-cooled, 200–230 VAC
50/60 Hz, no power cord
SV50239.05 ThermoFlex 2,500 TCU, SV50239.31 TCU hose kit 3/4 inch ID, 10 ft, SV50239.34 TCU ball valve kit right angle,
2.5 kW cooling 2.8 W heating, –5–50°C, 1 1/2 inch tri-clamp 1/2 inch NPT, ball valve
liquid-cooled, 200–230 VAC 50/60 Hz,
no power cord
5 kW cooling 2.8 W heating, –5–50°C, 1 1/2 inch tri-clamp 3/4 inch NPT, ball valve
air-cooled, 200–230 VAC 50/60 Hz,
no power cord
5 kW cooling 2.8 W heating, –5–50°C, 1 1/2 inch tri-clamp 3/4 inch NPT, ball valve
liquid-cooled, 200–230 VAC 50/60 Hz,
no power cord
SV50239.29 ThermoFlex 10,000 TCU, SV50239.37 TCU hose kit 1 inch ID, 10 ft, SV50239.41 TCU ball valve kit right angle,
10 kW cooling 6.1 W heating, –5–50°C, 1 1/2 inch tri-clamp 1 inch NPT, ball valve
air-cooled, 400/460 VAC 50/60 Hz,
no power cord
10 kW cooling 6.1 W heating, –5–50°C, 1 1/2 inch tri-clamp 1 inch NPT, ball valve
liquid-cooled, 400/460 VAC 50/60 Hz,
no power cord
24 kW cooling 22.5 W heating, –5–50°C, 1 1/2 inch tr-iclamp 1 inch NPT, ball valve
liquid-cooled, 400/460 VAC 50/60 Hz,
no power cord
ThermoFlex Chiller heating and cooling calculation* Cool from 37˚C to 5˚C Heat from 5˚C to 37˚C
Temperature Single-use
Volume Inlet psi Outlet psi RPM Minutes Hours Minutes Hours
control unit bioreactor
ThermoFlex 2,500 50 L 50 L 6.6 5.4 185 109 1.8 66 1.1
ThermoFlex 2,500 50 L 25 L 6.6 5.4 147 82 1.4 50 0.8
ThermoFlex 2,500 100 L 100 L 6.4 5.7 155 205 3.4 115 1.9
ThermoFlex 2,500 100 L 50 L 6.4 5.7 123 128 2.1 70 1.2
ThermoFlex 5,000 250 L 250 L 32.6 22.8 124 229 3.8 188 3.1
ThermoFlex 5,000 250 L 125 L 32.6 22.8 98 150 2.5 123 2.1
ThermoFlex 10,000 500 L 500 L 34.7 20.3 104 221 3.7 156 2.6
ThermoFlex 10,000 500 L 250 L 34.7 20.3 82 149 2.5 92 1.5
ThermoFlex 24,000 1,000 L 1,000 L 31.6 21.3 87 181 3.0 109 1.8
ThermoFlex 24,000 1,000 L 500 L 31.6 21.3 69 123 2.1 79 1.3
ThermoFlex 24,000 2,000 L 2,000 L 24.1 20.2 73 275 4.6 165 2.8
ThermoFlex 24,000 2,000 L 1,000 L 24.1 20.2 58 208 3.5 136 2.3
*Heating and cooling times based on full- and half-tank volumes.
Bioreactor controlcellsolutions
culture
u Thermo Scientific bioreactor control solutions
The Thermo Scientific from Applikon™ Bioreactor Control is

available in both the ez-Control (embedded) and Allen Bradley or Siemens
(PLC) platforms. The embedded system offers total bioreactor control
at your fingertips. This system is designed to accurately control pH,
temperature, dissolved oxygen (DO) and agitation. It can be used with a
single- or dual-bioreactor setup. The PLC system is a scalable control
that provides more advanced options than the embedded version. This
also can be operated in the single- or dual-bioreactor setup. This unit is
designed to effectively control processes from laboratory scale to pilot
plant and production scale.
Key features
Embedded platform system Associated products
• Adaptive PID Control • HyPerforma S.U.B. (Pg. 52)
• OPC Compliance • HyPerforma S.U.F. (Pg. 57)
• User-definable control loop configuration

• Multiple user level
• Small footprint, flexibility and accurate control
PLC platform system
• Off-the-shelf, standard PLC solution

• Local bioreactor control and display
• System documentation that facilitates validation
• Supports compliance to 21 CFR part 11
• Helps save time and money during scale-up process
65
Bioreactor control solutions
Applikon bioreactor control summary details
Summary Details
pH Operates with both traditional and single-use sensors
Temperature sensor Pt 100, 3 wire
Dissolved oxygen Operates with both traditional and single-use sensors
Foam/level Conductivity-based measurement
Analog in 8x available
Analog out 4x available
Digital in 8x available
Digital out 8x available
Biomass Capacitance or optical density (optional)
Display 12–15 in. color display with resistive touchscreen
1x Ethernet port (computer communication)
Communication interfaces 2x USB auxiliary
1x USB main
Control type PID control with selectable auto-tuning adaptive control
Up to 3 speed pumps; fixed-speed for embedded-based, variable-speed for PLC-based
Up to 4 mass flow controllers
Actuators
Heating and cooling via thermocirculator, heating blanket, cooling water valve
Water-cooled condenser connection
Additional features Emergency stop connection, central alarm output, color touch-screen operation
Power (EU/US) 230 VAC 50 Hz/115 VAC 60 Hz
Dimensions (H x W x L) 711 x 408 x 474 mm
Weight 30 kg
Bioreactor controlcellsolutions
culture
u Thermo Scientific Bioreactor Control Solutions
The Thermo Scientific from Finesse™ Bioreactor Control utilizes the

TruBio™ DV platform. This controller is a cart-mounted, self-contained
unit that offers modularity and allows the ability to add functional-
ity as your bioprocess evolves. This controller is designed to control
Thermo Scientific bioreactors and can be operated singly or
networked. This unit is ideal for easy, reliable and repeatable process
development and cell culture optimization.
Key features
G3Lite™ with TruBio DV platform
• Supports compliance to 21 CFR part 11

• Remote access notification
• Offers small footprint, flexibility and accurate control
• Ability to be networked
• Helps save time and money during scale-up process
Associated products

67
Bioreactor control solutions
Finesse Bioreactor control summary details
Control and settings Alarm management

pH control Alarm configuration
Dissolved oxygen control Alarm priorities
Weight control Alarm type
Auxiliary input control Remote access notification
Agitator speed control Recipe management
Gas flow control ISA 88 batch control (optional)
Pump control Recipe editor (optional)
Foam control Process data storage
Level control Continuous historian
Basic operations Audit trail
Process signal conditioning Calibration
Vessel settings pH sensor (one-point, two-point, slope/offset)
Key parameter display on configuration page DO sensors (one-point, two-point)
Online parameter update Temperature sensors (one-point, two-point)
Total (liquid and gas) pages Pressure sensors (one-point, two-point)
Analyzer data entry pages Pump
User access configuration Agitator speed (one-point, two-point)
Electronic signature Vessel weight (zero, tare)
Backup and restore procedure Auxiliary input
Features to assist with 21 CFR part 11 compliance
Miscellaneous
Interlock configuration
pH/DO sensor redundancy
Dual
Triple
Services and
cellsculture
support
u Thermo Scientific BioProduction services and support
Bioproduction services involve both field- and office-based teams.

The office-based team is located in Logan, Utah, and includes
technical support, BioProduction Xpress (BPX), quality and services
management teams. On a global scale, there are field application
specialists (FAS) who are trained on hardware installation, servicing and
also offer other post-sales support such as, training, consulting and
process optimization assistance.
BioProduction services and support key services:
Field support
• Hardware installation–Factory acceptance test (FAT) and site acceptance test (SAT)
• Training–Single-Use Bioreactors, Single-Use Mixers, Single-Use Fermentors, bins and
BPCs
• Validation service–Installation Qualification (IQ), Operation Qualification (OQ), and
Performance Qualification (PQ)
• Warranty
• Service contracts
BioProduction Xpress
• R&D scientists and engineers specializing in S.U.B., S.U.M. and S.U.F. processes
• Scale-up studies
• Customer-specific application studies
• Quality testing
• Non-GMP quick response studies or tests
Technical support
• Phone support for sales, service, technical questions, troubleshooting

• Interfaces with quality, product management and R&D teams
Associated products

• Large Volume Stainless Steel Outer Containers (Pg. 32)
69
Cell line development
cell culture
u Cell line development products
The Gibco™ Freedom™ CHO-S™ Kit, co-developed with ProBioGen AG, is an

easy-to-use, beginning-to-end product, for cloning and expression of recombinant proteins in
Chinese Hamster Ovary (CHO)-derived suspension culture. The Freedom CHO-S Kit includes
components for transfection, expression, clone creation and stable cell line selection, all in a
conveniently packaged kit. The Freedom CHO-S Kit also enables high titers, and offers simplified
commercial licensing options.
Key features
• Flexible commercial licensing without the need for royalties
• Ability to achieve IgG titers over 3 g/L
• Complete workflow that can take your gene of interest from transfection to lead clone
typically in 5 months
• Convenient packaging, for simplified ordering and storage
The Gibco™ Freedom™ DG44 Kit is an easy-to-use, beginning-to-end product, for

cloning and expression of recombinant proteins in dihydrofolate reductase deficient (DHFR-) CHO
cells. The Freedom DG44 Kit includes components for transfection, expression, clone creation,
and stable cell line selection, all in a conveniently packaged kit. The Freedom DG44 Kit also
enables a proven host cell line with simplified commercial licensing options.
Key features
• Flexible commercial licensing without the need for royalties
• Ability to achieve IgG titers over 3 g/L
• Complete workflow that can take your gene of interest from transfection to lead clone typically in
less than 6 months
• Convenient packaging, for simplified ordering and storage
Description Cat. No. Unit Size

Freedom CHO-S Kit A13696-01 1 kit
Freedom DG44 Kit A13737-01 1 kit
Need access to Gibco Freedom kit support?

If additional support is needed beyond the manual, you can contact your account manager to receive a Process Science Fellow
consultation. Don’t be surprised if one of our support scientists checks in with you to be sure your experience with the kit is as
successful as possible. For further assistance, please email our support team with your questions and contact information at
pd.direct@lifetech.com.
Cell culture
cell culture
media
u Cell culture media
sub-catagory
Gibco™ CD OptiCHO™ Liquid Medium and Gibco™ CD OptiCHO
AGT™ Medium are specifically designed to offer high performance and yield with
recombinant CHO cells in a chemically defined fed-batch environment. The protein–free, animal
origin–free formulations are designed to provide consistency and reduces the need to screen for
adventitious agents. CD OptiCHO Medium builds on Gibco™ CD CHO Medium and its legacy of
consistent cell growth and titers, while offering up to 40% better titer and cell growth over select
competitors. Thanks to its low osmolality and advanced Gibco™ CHO CD EfficientFeed™ Liquid
Nutrient Supplement Kit feeding options, CD OptiCHO Medium is capable of gaining a 2- to 5-
fold increases in fed-batch productivity over batch culture.
When to Use CD OptiCHO Medium?
Consider CD OptiCHO Medium when:
• You are using a transfected CHO cell line other than CHO K1, GS CHO or CHO-S cell lines
• Optimization of feeding strategies will be part of your base medium–selection testing in an
effort to maximize productivity
• Your CHO cell line is “finicky;” i.e., hard to grow or adapt
CD OptiCHO AGT Medium
Cat. No. Unit size

A11222-01 100 L
A11222-03 10 kg
A11222-04 1L
A11222-05 10 L
1X CD OptiCHO Liquid Medium
Cat. No. Unit size

12681-001 1,000 mL
12681-029 6 x 1,000 mL
71
Cell culture media
Gibco™ CD CHO Medium and Gibco™ CD CHO AGT™ (Advanced

Granulation Technology) Medium is protein-free, chemically-defined medium optimized
for the growth of CHO cells and expression of recombinant proteins in suspension culture. CD CHO
Medium and CD CHO AGT Medium contain no proteins or peptide components of animal, plant,
or synthetic origin, as well as no undefined lysates or hydrolysates. The AGT dry media format is
designed to provide increased consistency and productivity across all stages of production from
development to commercial manufacturing.
When to Use CD CHO Medium?
Consider CD CHO Medium when:
• Your GS CHO cells are recommended to be grown in CD CHO Medium

• Your cells have been successfully cultured in CD CHO Medium in the past and your process
is nearing a regulatory filing (DMF available)
1X CD CHO Medium
Cat. No. Unit size
10743-001 10 L
10743-002 20 L
10743-011 500 mL
10743-029 1,000 mL
CD CHO AGT Medium

Cat. No. Unit size
12490-001 100 L
12490-017 1L
12490-025 10 L
Cell culture
cell culture
media
Gibco™ Dynamis™ AGT™ dry format medium is specifically designed to offer the high
batch and fed-batch culture performance and yield with recombinant CHO cells in a chemically-
defined environment. The chemically-defined, protein-free, animal origin component–free
formulation provides the power to achieve high titers, start process development faster, and
streamline or simplify transfer to manufacturing scale.
When to Use Dynamis AGT Medium?
Consider Dynamis AGT Medium when:
• The cell line you are using is a transfected CHO K1, GS CHO, or CHO-S cell line
• Maximum batch culture cell densities and protein titers are needed
• You have time to adapt cells into a new medium in an effort to maximize titers
• Cell health at harvest is a priority for downstream processing
Dynamis AGT Medium

Cat. No. Unit Size
A2617501 10 L
A2617502 100 L
A2617503 10 kg
A2617504 1L
73
Cell culture feeds
u Cell culture feeds
Gibco™ EfficientFeed™ + Supplements are dry-format single-part supplements

for fed-batch addition to the culture of multiple cell lines to assist with process development
and maximization of bioreactor utilization. These products are an animal origin–free, chemically-
defined formulation that contains no proteins, hydrolysates, or components of incompletely defined
composition.
EfficientFeed A+ AGT Supplement EfficientFeed™ A+, B+, and C+ AGT™ Supplements

Cat. No. Unit Size Compared to original CHO CD EfficientFeed™ A AGT™ Nutrient Supplement, EfficientFeed™
A+ AGT™ Supplement has the ability to super-concentrate the supplement from 66.4 g/L (1X)
A25023-01 100 L to 199.2 g/L (3X) for reduction of product dilution or increased titers with additional feeding,
A25023-04 1L when appropriate. Compared to original CHO CD EfficientFeed™ B AGT™ Nutrient Supplement,
A25023-05 10 L EfficientFeed™ B+ AGT™ Supplement has the ability to super-concentrate the supplement from
52.7 g/L (1X) to 158.1g/L (3X) for reduction of product dilution or increased titers with additional
feeding, when appropriate. This product is designed to work with CHO cell lines expressing endog-
enous glutamine synthetase (GS) as a single feed without the need for additional GS supplements.
Compared to the original CD EfficientFeed™ C+ AGT™ Nutrient Supplement, EfficientFeed™
C+ AGT™ Supplement has the ability to super-concentrate the supplement from 81.2 g/L (1X) to
162.4 g/L (2X) for reduction of product dilution or increased titers with additional feeding, when
appropriate.
EfficientFeed B+ AGT Supplement

Cat. No. Unit Size Which EfficientFeed + Supplement to choose?
A25030-01 100 L
A25030-04 1L Consider EfficientFeed A+ AGT Supplement when:
A25030-05 10 L
• You have used CHO CD EfficientFeed A AGT Nutrient Supplement in the past and wish to
explore simpler reconstitution and super-concentration benefits of new technology for
delivery of key components
• You are currently using CD OptiCHO as your base cell growth medium
Consider EfficientFeed B+ AGT Supplement when:
• You have used CHO CD EfficientFeed™ B AGT Nutrient Supplement in the past and wish to
explore simpler reconstitution and super-concentration benefits of a newer technology for
delivery of key components
• You are currently using CD CHO as your base cell growth medium
EfficientFeed C+ AGT Supplement Consider EfficientFeed™ C+ AGT™ Supplement when:

Cat. No. Unit Size
• You have used CD EfficientFeed™ C AGT Nutrient Supplement in the past and wish to
A25031-01 100 L
explore simpler reconstitution and super-concentration benefits of new technology for
A25031-04 1L delivery of key components
A25031-05 10 L • You want higher productivity in GS CHO, CHO-K1 and CHO-S derived cell lines
• You are currently using CD FortiCHO™ Medium as your base cell growth medium
• You are using a base medium with greater than 6 g/L glucose or a hydrolysate at day 0
FunctionMAX TiterEnhancer
cell culture
u FunctionMAX TiterEnhancer
Gibco™ FunctionMAX™ TiterEnhancer uses a new technology to combine key

nutrients in a highly concentrated (150 g/L), pH-neutral, chemically-defined, AOF (animal origin–
free) functional additive that is designed to reinforce existing feeds and amplify the productivity of
standard high cell density fed-batch platforms. FunctionMAX TiterEnhancer is available in both this
liquid format and an AGT dry format and is stable for over 1 year.
When to use FunctionMAX TiterEnhancer?
Consider FunctionMAX TiterEnhancer when:
• You have a drop off in specific productivity late in a high density fed-batch culture;
FunctionMAX TiterEnhancer may be able to improve your titers
• You have an extremely low peak cell density fed-batch culture; you may want to consult with
PD-Direct™ Services for advice on cell adaptation and/or fed-batch optimization
• You have no drop off in specific productivity late in a high density fed-batch culture and
still need higher titers; you may want to consult with PD-Direct Services for advice on
fed-batch optimization
Key features
• Up to double prior fed-batch titers

• Highly concentrated ingredients
• Easy to use pH-neutral format
• Specialized delivery of ingredients found in base media—nothing exotic
• Use that is economical
FunctionMAX TiterEnhancer (liquid format)

Cat. No. Unit Size
A15010-01 100 mL
A15010-02 500 mL
FunctionMAX TiterEnhancer, AGT (powder format)

Cat. No. Unit Size
A15009-01 10 L
A15009-02 100 L
75
POROS and CaptureSelect purification products
u POROS and CaptureSelect purification products
We offer a broad portfolio of purification products supporting

biopharmaceutical development. These range from unique
chromatography resins used for primary capture and high-performance
polishing, to a robust analytical toolset for characterization and detection
of biological compounds. For large scale biomanufacturing we offer a
variety of products for affinity chromatography, ion exchange, reversed-
phase, and HIC applications designed to meet today’s biomolecule
purification challenges. Applied Biosystems™ POROS™ bulk chromatog-
raphy resins are used throughout the industry offering high-performance,
high-throughput process-scale bioseparations. Life Technologies™
CaptureSelect™ affinity products are available for process-scale or
analytical bioseparations for a wide variety of biotherapeutic compounds
including antibodies and antibody fragments, recombinant and plasma
proteins, and viral particles. Our custom ligand and resin discovery
programs generate customized CaptureSelect™ affinity ligands that can
be immobilized onto high-throughput POROS™ resins for your unique
separation needs.
Key features of POROS anion exchange resins

Compared to other commercially available anion exchange resins, POROS anion exchange resins
(POROS™ HQ 50, POROS™ PI 50 and POROS™ D 50 resins) are designed to have unique
surface chemistries that drive different selectivity, which can impact protein binding and separation.
The 50 μm average particle size enables enhanced resolution capability and the unique pore
structure helps bring benefits to small and large recombinant protein purification. POROS™ XQ
resin is a next-generation, high-capacity, high-resolution, salt-tolerant, strong anion exchange resin
that enables >140 mg/mL dynamic binding capacity in the presence of up to 6.6 mS/cm NaCl,
while helping deliver exceptional separation performance. A major improvement to the performance
of traditional anion exchangers, POROS XQ resin sets the standard for strong anion exchange
chromatography in bind-elute and flow-through modes of operation.
POROS anion exchange resins

Description Cat. No.
POROS™ XQ Strong Anion Exchange Resin 1,000 mL 4467818
POROS™ XQ Strong Anion Exchange Resin 5,000 mL 4467817
POROS™ HQ 50 µm 1 L 1-2559-07
POROS™ HQ 50 µm 5 L 1-2559-09
POROS™ HQ 50 µm 10 L 1-2559-08
POROS™ PI 50 µm 1 L 1-2459-07
POROS™ PI 50 µm 5 L 1-2459-09
POROS™ PI 50 µm 10 L 1-2459-08
POROS™ D 50 µm 1 L 1-3659-07
POROS™ D 50 µm 5 L 1-3659-09
POROS™ D 50 µm 10 L 1-3659-08
For more information, visit www.lifetechnologies.com/poros and www.lifetechnologies.com/captureselect
Key features of POROSTM cation exchange resins
POROS™ XS and HS strong cation exchange resins are rigid, polymeric cation exchange
chromatography resins that can be used to separate biomolecules including monoclonal
antibodies, recombinant proteins, and peptides. The resin backbone consists of cross-
linked poly[styrene-divinylbenzene]. A polyhydroxyl surface coating enables low non-specific
binding, and surface functionalization with sulphopropyl yields a strong cation exchanger
ionizable over the complete pH range. Designed especially for the separation of target proteins from
process impurities, POROS™ HS resin is a high-performance resin for cation polish
chromatography. POROS™ XS resin is designed to deliver greater than approximate 100 mg/mL
dynamic binding capacity for monoclonal antibodies and recombinant proteins, without sacrificing
superior resolution.
POROS cation exchange resins

POROS™ HS 50 µm 1 L 1-3359-07
POROS™ HS 50 µm 5 L 1-3359-09
POROS™ HS 50 µm 10 L 1-3359-08
POROS™ XS Cation Exchange Resin 25 mL 4404339
POROS™ XS Cation Exchange Resin 1,000 mL 4404336
Key features of POROS and CaptureSelect affinity resins
POROS™ MabCapture™ Protein A chromatography resin enables high dynamic binding

performance at low flow rates, while maintaining superior binding capacity at high linear velocities.
CaptureSelect affinity ligand technology enables the purification of antibodies, antibody
fragments and proteins with proven affinity solutions. These products enable increased purity and
yield and are designed to simplify research workflows and reduce time and cost in
biopharmaceutical drug development.
POROS and CaptureSelect affinity resins

POROS™ MabCapture™ A Resin 1 L 4374735
CaptureSelect™ IgG-CH1 Affinity Matrix 5 mL 194320005
77
u POROS and CaptureSelect purification products
Key features of POROSTM analytical chromatography columns
POROS Protein A, G and CaptureSelect™ analytical columns are used throughout the industry to
monitor monoclonal antibody titer and yield from cell culture supernatant, and can be operated on
any standard high performance liquid chromatography (HPLC) or medium pressure chromatography
system. POROS™ analytical chromatography columns, available in 10 and 20 micron particle size,
enable rapid separation for biomolecules for both analytical and lab-scale preparative
applications. POROS™ Protein A analytical columns are widely used in the industry for
monitoring monoclonal antibody titer and yield from cell culture supernatant. The addition of
POROS™ CaptureSelect™ analytical columns expand the advantages of high-speed,
high-performance quantitation to IgM, Fab fragments and fusion proteins.
POROS analytical chromatography columns

POROS™ A 20 µm Column, 10 x 100 mm, 7.9 mL (PEEK) 1-5022-46
POROS™ A 20 µm Column, 2.1 x 30 mm, 0.1 mL (PEEK) 2-1001-00
POROS™ A 20 µm Column, 2.1 x 30 mm, 0.1 mL (Stainless Steel) 1-5024-12
POROS™ CaptureSelect™ IgG Fc Affinity Column 2.1 x 30 mm 4469148
POROS™ CaptureSelect™ IgG Fc Affinity Column 4.6 × 50 mm 4469153
POROS™ CaptureSelect™ IgG Fc Affinity Column 4.6 × 100 mm 4469166
POROS™ CaptureSelect™ IgG Fc Affinity Column 10 × 100 mm 4469171
Applied Biosystems analytics
u Applied Biosystems analytics
Applied Biosystems™ SEQ analytical testing products are rapid

molecular methods for pharmaceutical manufacturing to help ensure
the quality and safety of your pharmaceutical products, especially when
accuracy and time-to-results are critical. These methods help detect
impurities and identify contaminants by using molecular techniques such
as DNA sequencing, PCR and real-time PCR, and are widely used for
pharmaceutical analytics worldwide.
Rapid molecular methods are also ideal for use in public health
laboratories, cosmetics and personal care products manufacturing, food
testing laboratories, and academic and research centers.
Key features of host cell residual DNA quantitation

The removal of host cell impurities is a critical step in the purification of biopharmaceutical
products. A major challenge is the accurate and sensitive quantitation of host cell DNA impurities in
both the purification process and in drug substance samples.
With resDNASEQ™ Residual DNA Quantitation Systems:
• Quantitate host cell residual DNA typically in under 5 hours with the resDNASEQ™
Residual DNA Quantitation Assays for human, CHO, E. coli, MDCK, Vero, NS0 and Pichia
pastoris cell lines
• Helps recover high levels of DNA from complex matrices with the PrepSEQ™ Residual
DNA Sample Preparation Kit
• Analyze results with AccuSEQ™ Real-Time PCR Detection Software
ResDNASEQ residual DNA quantitation kits

resDNASEQ™ Quantitative CHO DNA Kit 4402085
resDNASEQ™ Quantitative CHO DNA Kit with PrepSEQ™ Residual DNA Sample 4413713
Preparation Kit
resDNASEQ™ Human Residual DNA Quantitation Kit A26366
resDNASEQ™ Human Residual DNA Quantitation Kit with PrepSEQ™ Residual A27335
DNA Sample Preparation Kit
resDNASEQ™ Quantitative E. coli DNA Kit 4458435
resDNASEQ™ Quantitative E. coli DNA Kit with PrepSEQ™ Residual DNA Sample 4460366
Preparation Kit
For more information, visit www.lifetechnologies.com/pharmaanalytics
79
u Applied Biosystems analytics
Key features of host cell protein quantitation
The ProteinSEQ™ Protein Quantitation System is a breakthrough immuno-PCR–based method

designed for sensitive and robust protein quantitation with 4 logs of dynamic range, providing
greater confidence and quality of data throughout the quantitation process.
• PCR sensitivity—Quantitation to as low as 0.2 ng/mL generic host cell protein (HCP)
• Wide dynamic range—4 logs
• Designed for robustness—dilution linearity and efficiency in complex matrices
• Semi-automation—2-hour walk-away time
ProteinSEQ Protein Quantitation Kit

ProteinSEQ™ CHO Host Cell Protein Quantification Kit A27601
Key features of microbial identification
Harmful bacteria and fungi can contaminate raw materials, production facilities, and branded
products. The use of a phylogenetic approach for microbial detection based on the 16S rDNA gene
for bacteria or a specific genomic region of the large subunit for fungi can help prevent delayed
product releases, back orders and even recalls.
Identify thousands of bacterial and fungal species typically in under 5 hours with the MicroSEQ™
Microbial Identification System.
MicroSEQ ID systems
MicroSEQ™ Rapid Microbial ID System II with 3500 Genetic Analyzer 4462993
MicroSEQ™ Rapid Microbial ID System II with 3500xL Genetic Analyzer 4462992
Fast MicroSEQ™ 500 16S rDNA PCR Kit 4370489
MicroSEQ™ D2 rDNA Fungal PCR Kit 4349153
Key features of Mycoplasma Detection
The MycoSEQ™ Mycoplasma Detection System integrates real-time PCR assays, instruments, and
software with optimized PrepSEQ™ sample preparation reagents– enabling results in hours, not
days. This enables you to test at multiple points in cell lines, cell banks, and harvest media, helping
to increase your productivity and reduce risk of downstream contamination.
• Rapid time-to-results typically in less than 5 hours

• Detection of more than 90 Mycoplasma species
• Demonstrated sensitivity to detect less than 10 copies⁄reaction
• Offers proven specificity
• PrepSEQ sample preparation enables high efficiency DNA recovery
• Proprietary discriminatory positive⁄extraction control
MycoSEQ™ Mycoplasma detection kits

MycoSEQ™ Mycoplasma Detection Kit, with Discriminatory Positive Control 4460623
MycoSEQ™ Mycoplasma Detection Kit, with Discriminatory Positive Control & 4460626
sample preparation
81
u Applied Biosystems™ analytics
Key features of adventitious virus testing

MMV and Vesivirus contamination is a potential threat to mammalian cell culture manufacturing
processes. Multiple cell culture manufacturing facilities have been negatively impacted over the
past 20 years following contamination with these viruses. The ViralSEQ™ Detection System is an
integrated DNA purification and real-time PCR-based test designed for rapid, sensitive detection of
MMV and Vesivirus from a wide range of sample types.
• Proven TaqMan™ technology for highly sensitive detection down to 20 genome copies per
reaction (viral particles equivalent to 0.002 TCID50)
• Specific, no cross-reactivity with unrelated DNA
• Discriminatory positive/extraction DNA control to help minimize the possibility of false
positive test results from accidental cross-contamination with control DNA
• Use with PrepSEQ™ 1-2-3 Nucleic Acid Extraction Kit, designed for optimized, highly
efficient DNA recovery
• Rapid time-to-results, typically in less than 4 hours
ViralSEQ detection kits

ViralSEQ™ Vesivirus Detection Kit 4448398C
ViralSEQ™ Mouse Minute Virus (MMV) Detection System 4444415
Key features of AccuSEQTM Real-Time PCR Detection Software
The AccuSEQ Real-Time PCR Detection Software is part of a system that performs analytical testing
for impurities and contaminants during the biopharmaceutical manufacturing process. Designed
to work with the 7500 Fast Real-Time PCR instrument, the software supports the SEQ family of
analytical detection and quantitation assays. The AccuSEQ Software offers a plug-in analysis
module for automated presence⁄ absence calls when using the MycoSEQ Mycoplasma Detection
System, while a custom module built into the main application can be used with the resDNASEQ
CHO residual DNA quantitation system.
• Automated presence⁄absence calls for Mycoplasma detection

• Enables accurate quantitation of host cell line residual DNA
• Single software platform for current and future SEQ Real-Time PCR assays
• Helps enable 21 CFR Part 11 compliance
Software
AccuSEQ 2.0 Real-Time PCR Detection Software 4443420
Key features in sample prep and automation
PrepSEQ™ chemistry is a time-saving, cost-effective universal sample preparation method used

in multiple applications, including residual host cell DNA quantitation, mycoplasma detection, and
viral detection. It offers high quality performance for quantitative recovery, high reproducibility, and
consistent performance across a broad range of complex matrices.
Applied Biosystems™ MagMAX™ Express-96 and AutoMate Express™ systems offer labs a
boost in productivity by helping to reduce hands-on sample preparation time, increase throughput,
reduce costs, and improving the quality of sample extractions. Intuitive to operate and pre-loaded
with protocols, our automated sample preparation instruments help meet your throughput needs.
• AutoMate Express™ Nucleic Acid Extraction System—Ideal for in-process testing

of contaminant and impurities, this closed system offers out-of-the-box automation with
prefilled PrepSEQ™ Express cartridges to minimize the risk of cross-contamination
• MagMAX™ Express-96 Magnetic Particle Processor—High-throughput 96-well
format
Sample prep and automation

PrepSEQ™ 1-2-3 Nucleic Acid Extraction Kit 4452222
PrepSEQ™ Express Nucleic Acid Extraction Kit 4466351
AutoMate™ Express Nucleic Acid Extraction System 4467754
MagMAX™ Express-96 Deep-Well Magnetic Particle Processor 4456933
For more information, go to www.lifetechnologies.com/pharmaanalytics
83
Find out more at lifetechnologies.com/sut
© 2015 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo
Fisher Scientific and its subsidiaries unless otherwise specified. Allen-Bradley is a trademark of
Allen-Bradley Company. ALLpaQ is a registered trademark of ALLpaQ Packaging Limited. Applikon
is a trademark of Applikon B.B. AseptiQuik is a registered trademark of Colder Products Company.
ASTM is a registered trademark of the American Society for Testing Materials Corporation. BioTote
is a registered trademark for Plastic Concepts, Inc. C-Flex, PharMed, and Tygon are registered
trademarks of Saint-Gobain. Clave is a registered trademark of ICU Medical. Domnick Hunter is
a registered trademark of Parker Hannifin Corporation. EMD Millipore, Millipak and Opticap are
registered trademarks of Merck KGaA, Darmstadt, Germany. Emflon, Fluorodyne, Kleenpak and Pall
are registered trademarks of Pall Corporation. Envirotainer is a registered trademark of Enviro-
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a trademark of General Electric Company. Sartorius and Sartopore are trademarks of Sartorius AG.
Siemens is a registered trademark of Siemens Aktiengesellschaft. SmartSite is a registered trade-
mark of ALARIS Medical Systems, Inc. SterilEnz is a trademark of PAW BioScience Products, Inc.
Turck is a registered trademark of TURCK Inc. UltraCap and SteriLUX are a registered trademark of
Meissner Filtration Products, Inc. ZenPure is a registered trademark of ZenPure Corporation.
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Thermo Scientific

Introduction to Thermo Scientific BioProcess Containers............................................................................................................... 2

Products and Services

u Introduction to Thermo Scientific BioProcess Containers

Support operation Applications BPC solution

Hydration in closed/sterile system Mixtainer™ BPC, Single-Use Mixer (S.U.M.)

Custom tubing sets

u Introduction to Thermo Scientific BioProcess Containers

Unit operation Application BPC solution

Fermentor Fermentation in stirred-tank system Single-Use Fermentor (S.U.F.)

Custom BPCs to cover transfer assemblies

Custom BPCs to cover transfer assemblies

Transfer assemblies to transfer liquid from reservoir to filling system.

Custom BPCs to cover additional options

• All chambers and BPCs are produced in state-of-the-art cGMP facilities

BPC manufacturing process

The two main types of basic BPC chamber are:

u Thermo Scientific BPC manufacturing sites

Assembly Center, Logan, Utah

BioCenter, Logan, Utah

Our BioCenter includes:

Chamber manufacture—5 automated production lines manufacture the majority of our 2D

Final assembly—Although primarily a chamber manufacturing facility, our BioCenter serves

Chamber manufacturing—We manufacture both Aegis and CX Film-based BPC chambers.

u Quality control and assurance

Component incoming inspection

Final BPC product inspection

Lot record and Certificate of Analysis

u Quality control and assurance

Endotoxin and particulate

EMA/410/01 rev. 3 compliance

BPC validation documentation

BPC system hardware documentation

u Thermo Scientific CX5-14 Film

0.8 Property Test protocol Average values

Schematic 3D view Non-volatile residue USP <661> <1 mg

u Thermo Scientific CX3-9 Film

Polyester 0.8 Property Test protocol Average values

u Thermo Scientific Aegis5-14 Film

Polyester 0.8 Property Test protocol Average values

Tie 0.9 Physical data

u Thermo Scientific tubing selection guide

Although it is not practical to include the complete range of commercially

Key factors in selection of tubing for inclusion in a BPC systems are:

• Pumping characteristics—Peristaltic pumping is the most common method so the

PharmaPure™ This is supplied by Saint-Gobain™ as an alternative to Long pump life is required.

u Thermo Scientific connection system selection guide

SmartSite connection Clave Information not available N/A

u Thermo Scientific filter selection guide

Filtration is an important step in bioprocessing and a variety of filters are

Some major characteristics relevant to filter selection:

Supplier Type Material Final filter pore sizes (µm)

PROPOR™ SG, DOMICAP™ capsule PES 0.1, 0.2

u Thermo Scientific fluid transfer assemblies

Building a custom fluid transfer system? We are your ultimate customer

• A complete end-to-end solution provider

• Your trusted guide for bioprocess applications

• Delivering the benefits of single-use technology, customized

• Thermo Scientific™ HyPerforma™ Smartainer™Systems have chambers constructed