NUEVA ECIJA UNIVERSITY OF SCIENCE AND TECHNOLOGY

COLLEGE OF ARTS AND SCIENCES

COLLEGE OF NURSING
MICROBIOLOGY AND PARASITOLOGY (MC3) LABORATORY
POST LABORATORY REPORT IN

ESTIMATING THE NUMBER OF BACTERIA IN WATER

BAJA, JOAN R.
BSN 1-G
MARCH 24, 2020

INTRODUCTION

Large numbers of bacteria and viruses when seeded into household toilets were shown to remain in the

bowl after flushing, and even continual flushing could not remove a persistent fraction. This was found to be

due to the adsorption of the organisms to the porcelain surfaces of the bowl, with gradual elution occurring

after each flush. Droplets produced by flushing toilets were found to harbor both bacteria and viruses which

had been seeded. The detection of bacteria and viruses falling out onto surfaces in bathrooms after flushing

indicated that they remain airborne long enough to settle on surface throughout the bathroom. Thus, there is a

possibility that a person may acquire an infection from an aerosol produced by a toilet. (Gelba et al, 1975)

According to Korin miller, Toilet plume is a term for what happens when the force of flushing sprays

microscopic particles of pee, poop, and whatever else is in the bowl into the air. The experiment will conclude

whether the results will validate the procedural and assay the outcome of the experiment. The postulation

within ourselves will proves the development on how it reacts towards the area of the petridish with the help of

the agar medium that expands the spread of the microbes through the plates.

METHODS

Using a sterile syringe, aspirate 5ml sample

Lift the lid and place sample in it. close the lid

Pour the agar and mix through the petridish

Allow the agar to set

Count the colonies present after incubation

Figure 1: General Procedure of Estimating the Number of Bacteria in Water

POST LABORATORY REPORT IN BIOCHEMISTRY (MC2) rtae
RESULTS

In this section, it represents the following images of the procedure for the experiment throughout the

outcome and verification that we all participated on this laboratory experiment.

All required substances will be The samples of the toilet

inoculated for the beginning of water from the syringe will be
the experiment. injected to the plates.

Pour the agar medium onto the

The petridish were heated
plates to arise the spread of the
for the agar medium to be
microbes.
stricken the comer areas.

IMAGE DOCUMENTS

POST LABORATORY REPORT IN BIOCHEMISTRY (MC2) rtae

CONCLUSION

It is to include the experiment number 2 about Estimating the number of bacteria in water Before

starting each group take a water example on the toilet each flour of neust campus after the autoclave after

agar and pentridish here in this experiment you will know what toilet is the toilet n super amount of bacteria that

will affect our health if we use the toilet after the autoclave puts the sample of water in the pentridish with agar

finish and allows the agar to set and incubation and count the calories present after incubation.

REFERENCE:

Vriesekoop et al (2016). Dirty Money: A Matter of Bacterial Survival, Adherence, and Toxicity. Retrieved from:

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5192525/

Santo, Morais, and Grass (2010). Isolation and Characterization of Bacteria Resistant to Metallic Copper

Surfaces. Retrieved from: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2832357/

POST LABORATORY REPORT IN BIOCHEMISTRY (MC2) rtae