V.No:1.

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Bioglucanase L
Glucan Gobbler Enzyme

Product Information
Description

Bioglucanase L is a thermo stable fungal beta glucanase enzyme, derived from

a strain of Trichoderma reesei, that has both beta 1 - 3 and beta 1 - 4
endoglucanase activities. It digests high molecular weight beta glucans in cereals
like barley and can be used in the treatment of barley / malt either during malting
or during mashing in the Brew house. Bioglucanase L complies with the JECFA
and FCC recommended specifications for food grade enzymes

Purpose

The barley endosperm cell wall contains about 70% beta glucan that undergoes
several modifications during malting. Addition of Bioglucanase L during malting
offers solution to many problems associated with beta glucans.

Use of Bioglucanase L during mashing results in hydrolysis of beta glucans

resulting in elimination of several associated problems.

Benefits

Bioglucanase L when used during malting and brewing gives following

advantages:

1. Increased degree and rate of modification of beta glucans during malting.

2. Increased brewery extract by 2 to 5%
3. Reduced mash viscosity by 0.15 - 0.25 cp, thereby increasing the speed of
lautering.
4. Preventing survival of beta glucans in finished beer which otherwise give rise
to filtration and haze problems.

Specification

Appearance : Brown liquid

Activity : min. 700 BG u/mL
Grade : Food Grade, Kosher
pH : 4.8 – 5.2
Specific Gravity : 1.0 – 1.2 g/ml
 Application Information

Recomendations

Bioglucanase L may be applied in the following way during malting or mashing :

1. Spray on the malt along with Giberillic acid at the beginning of the
germination process.
2. Add as a fine spray the day before kilning.
3. Add as a fine spray as the malt is leaving the kiln.
4. Add as slurry in the mash tun.

Dosage

The recommended dose rate of Bioglucanase L is 0.03 - 0.06% (w/w on malt) in

brewhouse and 0.01 – 0.03% (w/w on barley) in malthouse. However, it is
recommended to optimize the enzyme dosage based on plant conditions.

Effect of pH

The pH of Bioglucanase L is approximately 3.5 – 6.5, with an optimum

performance at pH 5.5.

Effect of Temperature

The effective temperature range of Bioglucanase L is 40 deg. C to 75 deg. C

with an optimum performance at 65 deg. C.

Stability

Bioglucanase L should be store in a cool dry place ( < 25 deg C) and sheltered
against direct sunlight. The declared enzyme activity will be maintained for one
year under these conditions.

Packaging

Bioglucanase L is available in standard 25 kg HDPE drums or may be custom

packed on request.
.
Technical Services

Further queries regarding specific application information can be obtained from

our technical services department.

Bioglucanase L
 Material Safety Data Sheet

1. PRODUCT AND COMPANY IDENTIFICATION

Product name : Bioglucanase L
Supplier : Biocon India limited
20th K.M Hosur Road , Hebbagodi
Bangalore District – 561229, INDIA
Tel: +91-8110-33356/32169.
Fax: +91-8110-32623

2. COMPOSITION /INFORMATION ON INGREDIENTS

Description : Liquid enzyme preparation
Chemical nature : Beta-Glucanase

3. HAZARDS IDENTIFICATION
Repeated inhalation of enzyme aerosol may cause sensitisation and will
cause allergic type responses in sensitised individuals.Prolonged skin contact
mau cause skin irritation.

4. FIRST AID MEASURES

Inhalation – remove from exposure. If irritation persists or allergic response
develops consult a doctor.
Skin contact – wash skin with plenty of water.
Contact with eyes – irrigate eyes with cold water for 20-min atleast. Obtain
medical advice if irritation occurs.
Ingestion – rinse mouth thoroughly with water and drink plenty of water
afterwards. Consult a doctor.

5. FIRE FIGHTING MEASURES

Water. Foam

6. HANDLING & STORAGE

Drums should be kept closed and in a dry and cool place. Use local exhaust
ventilation and/or personal protective equipment as necessary to minimise
exposure.

Contd..
 Ver.1.00/00

Material Safety Data Sheet

7. EXPOSURE CONTROLS / PERSONAL PROTECTION

Handle in well ventilated conditions in accordance with good industrial
hygiene and safety practices.
 Wear suitable overalls.
 Wear suitable gloves and eye protection.
 No eating, drinking or smoking in the work place.
 Practice good personal hygiene.
 Avoid inhalation of dust.

Wear suitable respiratory protective equipment if working in confined places

with inadequate ventilation or whenever there is any risk of the exposure
limits being exceeded.

8. PHYSICAL & CHEMICAL PROPERTIES

Colour / Physical state : Brown liquid
Flash Point : Not applicable
Solubility : Soluble in water

9. TOXICOLOGICAL INFORMATION
Repeated inhalation of enzyme aerosol may cause sensitisation and will
cause allergic type reactions in sensitised individuals. Prolonged skin contact
may cause irritation.

10. ECOLOGICAL INFORMATION

Product has no adverse effect on the environment.

11. TRANSPORT INFORMATION

No special packaging requirements. Not classified as dangerous goods.

12. OTHER INFORMATION

The information contained in this safety data sheet, as of the issue date, is
believed to be true and correct. However, the accuracy and completeness of
this information and any recommendations and suggestions are made without
warranty or guarantee. Since the conditions of use are beyond the control of
our company, it is the responsibility of the user to determine the conditions of
safe use of this product. The information in this sheet does not represent
analytical specifications, for which please refer to our technical data sheet.