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Bioglucanase
Fungal Beta Glucanase for Beer

Product Information

Description
BIOGLUCANASE is a thermostable fungal beta glucanase enzyme that has both
beta 1 - 3 and beta 1 - 4 endoglucanase activities.

BIOGLUCANASE digests high molecular weight beta glucans in cereals like barley
and can be used in the treatment of barley / malt either during malting or during
mashing in the Brew house.

Purpose

The barley endosperm cell walls contain about 70% beta glucan that undergoes
several modifications during malting. Addition of BIOGLUCANASE during
malting offers solution to many problems associated with beta glucans.

Use of BIOGLUCANASE during mashing results in hydrolysis of beta glucans

resulting in elimination of several associated problems.

Benefits

BIOGLUCANASE when used during malting and brewing gives following

advantages:

1. Increased degree and rate of modification of beta glucans during malting.

2. Increased brewery extract by 2 to 5%

3. Reduced mash viscosity by 0.15 - 0.25 cps, thereby increasing the speed of
lautering.

4. Preventing survival of beta glucans in finished beer which otherwise give rise to
filtration and haze problems.
 Application Information

BIOGLUCANASE may be applied in the following way during malting or mashing:

1. Spray on the malt along with Giberillic acid at the beginning of the germination
process.

2. Add as a fine spray the day before kilning.

3. Add as a fine spray as the malt is leaving the kiln.

4. Add as slurry in the mash tun.

The recommended dose rate of BIOGLUCANASE is 0.2 - 0.5% based on weight

adjunct and for all the malt systems BIOGLUCANSE should be used at 0.03 – 0.05%
of the malt weight .

Effect of pH:

The optimum pH of BIOGLUCANASE is 4.5 to 6.5

Effect of Temperature:

BIOGLUCANASE can be used upto 80 deg.C but above this temperature the
enzyme is rapidly inactivated. The optimum temperature for this enzyme is 60 to 70
deg. C.

Stability:

BIOGLUCANASE powder should be store in a cool dry place (< 25 deg C). The
declared enzyme activity will be maintained for one year under these conditions.

Packing:

BIOGLUCANASE is available in standard 25-kg fiberboard drums or may be

custom packed on request.

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Bioglucanase
Material Safety Data Sheet

1. PRODUCT AND COMPANY IDENTIFICATION

Product name : Bioglucanase
Supplier : Biocon India limited
20th K.M Hosur Road , Hebbagodi
Bangalore District – 561229, INDIA
Tel: +91-8110-33356/32169.
Fax: +91-8110-32623

2. COMPOSITION /INFORMATION ON INGREDIENTS

Description : Powder enzyme preparation
Chemical nature : Beta Glucanase

3. HAZARDS IDENTIFICATION
Exposure to any kind of dust is potentially harmful. Avoid inhalation and contact
with eyes and skin.

4. FIRST AID MEASURES

Inhalation – remove from exposure. If irritation persists or allergic response
develops consult a doctor.
Skin contact – wash skin with plenty of water.
Contact with eyes – irrigate eyes with cold water for 20-min atleast. Obtain
medical advice if irritation occurs.
Ingestion – rinse mouth thoroughly with water and drink plenty of water
afterwards. Consult a doctor.

5. FIRE FIGHTING MEASURES

Not applicable. Amorphous powder will not support combustion.

6. HANDLING & STORAGE

Drums and bags should be kept closed and in a dry place. Use local exhaust
ventilation and/or personal protective equipment as necessary to minimise
exposure.

Contd..

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Material Safety Data Sheet

7. EXPOSURE CONTROLS / PERSONAL PROTECTION

Handle in well ventilated conditions in accordance with good industrial hygiene
and safety practices.
 Wear suitable overalls.
 Wear suitable gloves and eye protection.
 No eating, drinking or smoking in the work place.
 Practice good personal hygiene.
 Avoid inhalation of dust.

Wear suitable respiratory protective equipment if working in confined places with

inadequate ventilation or whenever there is any risk of the exposure limits being
exceeded.

8. PHYSICAL & CHEMICAL PROPERTIES

Colour / Physical state : Beige coloured powder
Flash Point : Not applicable
Density : Not available
Solubility : Soluble in water

9. TOXICOLOGICAL INFORMATION
Repeated inhalation of dust may cause sensitisation and will cause allergic type
reactions in sensitised individuals. Prolonged skin contact may cause irritation.

10. ECOLOGICAL INFORMATION

Product has no adverse effect on the environment.

11. TRANSPORT INFORMATION

No special packaging requirements. Not classified as dangerous goods.

12. OTHER INFORMATION

The information contained in this safety data sheet, as of the issue date, is believed
to be true and correct. However, the accuracy and completeness of this
information and any recommendations and suggestions are made without warranty
or guarantee. Since the conditions of use are beyond the control of our company, it
is the responsibility of the user to determine the conditions of safe use of this
product. The information in this sheet does not represent analytical specifications,
for which please refer to our technical data sheet.
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