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Test Series For NEET - 2020
Test Series For NEET - 2020
Test Series For NEET - 2020
Code - A
M.M.: 720 Time: 3 Hrs.
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8. A ray of light falls on a transparent sphere with 13. A small aperture is illuminated with parallel beam of
center at C as shown in figure. If refractive index of light having wavelength = 942 nm. If first minima is
4 formed at an angular position of 1.5°, then size of
the sphere is . Then net deviation of the ray will be aperture is
3
(1) 18 m
A C B (2) 36 m
53° (3) 9 m
(4) 27 m
(1) 16° (2) 30°
14. The displacement of two interfering light waves at x = 0
(3) 32° (4) 60°
4 4 2
9. A converging lens of power 4D is placed in contact is given by y 1 sin t and y 2 sin t
with a diverging lens of power –2D. The nature and 5 5 3
focal length of this combination will be respectively. The amplitude of resultant wave will be
(1) Diverging, 50 cm (2) Converging, 50 cm
3 2
(3) Converging, 25 cm (4) Diverging, 25 cm (1) (2)
5 5
10. A point object is placed in front of a convex mirror
of focal length f0. Which one of the following graphs 4 1
correctly represent the variation of magnification (m) (3) (4)
5 5
of mirror with image distance v from the pole of
mirror? 15. In Young’s double-slit experiment, the intensity of
(1) m (2) m light at a point on screen, where path difference is
1
is I. The intensity of the light where the path
4
0 f0 v v
0 f0 difference is will be
–1 6
(3) m (4) m
(1) I (2) 2I
1
I 3I
(3) (4)
v 0
v 2 2
0
–1
16. Upto what distance is ray optics, a good
approximation when the aperture is 4 mm wide and
11. A point source of light at a finite distance from the wavelength is 800 nm?
source produces
(1) 20 m (2) 10 m
(1) Plane wavefront
(3) 8 m (4) 18 m
(2) Spherical wavefront
17. In Young’s double slit experiment a mica plate of
(3) Cylindrical wavefront refractive index is introduced in the path of light
(4) Depends on the colour of light coming from one of the slit. If the central bright fringe
shift to the point originally occupied by fourth bright
12. If the amplitude of transmitted polarised light through
fringe. Then thickness of the mica plate will be
A (Symbol have their usual meaning)
a polariser is , then the amplitude of unpolarised
2
SPACE FOR ROUGH WORK 2 4
light incident on it is (1) (2)
( – 1) 3( – 1)
A
(1) (2) A
2 4 2
(3) (4)
( – 1) 3( – 1)
(3) 2A (4) 2A
2/16
ALLEN
18. The critical angle for a transparent crystal-air medium Vo/p Vo/p
is 60°. Then its polarising angle is (1) (2)
o t o t
–1 1 Vo/p
(1) cot –1(2) (2) cot Vo/p
2
(3) (4) o t
3 o t
2
(3) tan–1 (4) tan–1
3 2 24. The current gain of the common base n-p-n transistor
is 0.96. If it is used as common-emitter amplifier,
19. In a forward biased p-n junction diode
output resistance 4000 input resistance 1000 ,
(1) Net current in the diode only due to diffusion
then voltage gain of the amplifier will be
current
(2) Net current in the diode only due to drift current (1) 24 (2) 96
(3) Net current is sum of magnitudes of diffusion (3) 48 (4) 72
current and drift current 25. The I-V characteristic of a photo diode is
(4) Net current is difference of magnitudes of
I
diffusion current and drift current (1) (2) I
20. Which logic gate is represented by the following O
combination of logic gates? V V
O
A
(3) I (4) I
Y
VOC
V
B O
O V
(1) OR gate (2) AND gate ISC
(3) NOR gate (4) XOR gate 26. For a transistor in active region
21. Which of the following diode is reverse biased? (1) The base region must be very thick and highly
doped
(1) (2)
+5V +2V –2V (2) Both the emitter base junction as well as base-
collector junction are forward biased
(3) (4)
–2V – 4V –3V –2V (3) Base, emitter, and collector region should have
22. A p-n junction diode is connected in parallel to a similar size and doping concentration
3 resistance in the circuit as shown in figure. If (4) The emitter-base junction is forward biased and
potential drop across the diode is 0.3 V. Then current base-collector junction is reversed biased
through the 7 resistance will be
27. The real time variation of input signal A and B are as
7 shown in figure. If the inputs are fed into NOR gate,
then select the correct output signal.
6.6 V 3
A t A
Y
(1) 0.3 A (2) 0.7 A B t B
(3) 0.9 A (4) 0.5 A O t1 t2 t3 t4
23. A rectifier circuit along with input is as shown in
(1) (2)
figure. The output waveform across RL is given by
(Consider diodes are ideal) Y
t Y t
D1
SPACE FOR ROUGH WORKO t1 t2 t3 t4 O t1 t2 t3 t4
VI/P
(3) (4)
o t
RL Y
Y t t
D2 O t1 t2 t3 t4
O t1 t2 t3 t4
3/16
ALLEN
28. An P-type semiconductor is 33. In photoelectric effect, the electron are ejected from
metals, if the incident light has a certain maximum
(1) Positively charged
(1) Wavelength (2) Amplitude
(2) Electrically neutral
(3) Frequency (4) Angle of incidence
(3) Negatively charged 34. If the kinetic energy of a proton gets doubled, then
(4) Positively or negatively charged depending upon the de-Broglie wavelength associated with it change
the amount of impurity by a factor
29. A point source of light is placed at a distance of 1 1
2 m from a photocell and cut-off potential is found to (1) (2)
2 3
be V 0 . If distance between point source and
photocell is halved, then cut-off potential will be 1
(3) 2 (4)
(1) V0 (2) 4V0 2
35. The de-Broglie wavelength of a particle accelerated
V with 100 volt potential difference is 10–9 m. The
(3) 2V0 (4) 0
2 de-Broglie wavelength of particle if it is accelerated
by 1600 volt potential difference will be
30. A photon of energy E0 falls normally on a perfectly
(1) 25 Å (2) 2.5 Å
absorbing surface. The momentum transferred to the
surface is (3) 5 Å (4) 0.5 Å
36. The velocity of most energetic electron emitted from
2E0 E0 a metallic surface is tripled when the frequency of
(1) (2)
c c the incident radiation is doubled. The work function
of this metal will be (h is Planck’s constant)
E0 3E0 5h 3h
(3) (4) (1) (2)
2c 2c 8 8
31. A particle of mass M at rest explodes into two parts
7h h
A and B having masses m and 2m respectively. The (3) (4)
8 4
ratio of de-Broglie wavelength of part A and B will be
th
37. In the n state, energy of electron in hydrogen atom
(1) 1 : 2 (2) 2 : 1
13.6
(3) 1 : 1 (4) 1: 2 is En = – eV . The potential energy of electron
n2
32. Graph of cut-off potential (V0) versus frequency (f ) for in first excited state will be
same intensity of light for metal plates A and B is (1) –27.2 eV (2) –3.4 eV
as shown in figure. Choose the correct statement. (3) –3.2 eV (4) – 6.8 eV
38. The ratio of maximum to minimum wavelength in
cut-off A B Lyman series is
potential (1) 9 : 5 (2) 4 : 3
(3) 36 : 5 (4) 4 : 1
O frequency(f ) 39. When an electron is in 4th excited energy state in
hydrogen atom, the possible number of emission
spectral lines is
(1) Work function of metal A is greater than work
function of metal B (1) 6 (2) 10
(3) 8 (4) 20
(2) Work function of metal A is less than work
function of metal B 40. In Rutherford’s experiment the number of -particle
SPACE FOR ROUGH WORK
scattered through an angle of 60° is 20 per minute.
(3) Work function of metal A is equal to work
Then the number of -particle scattered through an
function of metal B
angle of 90° per minute by the same nucleus is
(4) Both line have same intercept
(1) 12 (2) 10
(3) 5 (4) 16
4/16
ALLEN
[ CHEMISTRY]
46. Which one is most reactive towards SN1 reaction? CH3
(1) C6 H5CH2Br (2) (C6H5)2CH Br H OH
(3) (4) Mixture of (1) and (2)
(3) (C6H5)3C Br (4) C6H5Br H OH
47. 2-Bromo-3-methylpentane is heated with potassium CH3
ethoxide in ethanol. The major product obtained is 50. Which one is most acidic?
(1) 3-Methylpent-2-ene
OH OH
(2) 2-Ethoxy-3-methylpentane
(3) 2-Methylpent-2-ene NO2
(1) (2)
(4) 4-Methylpent-2-ene
48. Consider the reaction.
CH3CH2CH2Br + CH3MgBr OH OH
CH3CH2CH2CH3 + MgBr2 NO2 O2N NO2
The above reaction can occur in which of the (3) (4)
solvent?
(1) H2O (2) Acetone NO2 NO2
(3) Ethanol (4) Tetrahydrofuran
51. Propene on reaction with B 2 H 6 f ollowed by
CH3 CH3 H2O2/OH– gives A. The compound A on treatment
Br2 /CCl4 Zn/ether (i) OsO4
49. C == C A B (ii) NaHSO C
3
with Na gives B and with PCl5 gives C. B and C
H H
react together to form
The product C is SPACE FOR ROUGH WORK
(1) CH3CH2CH2OH
CH3 CH3
(2) CH3CH2OCH3
H OH HO H
(1) (2) (3) CH3CH2CH2OCH2CH2CH3
HO H H OH
CH3 (4) CH3CH2CH2CH2CH2CH3
CH3
5/16
ALLEN
52. On treating phenol with chloroform in the presence (1) Formation of intramolecular H-bonding
of sodium hydroxide, the intermediate formed is (2) Weak molecular association arising out of the
dipole-dipole interaction
OH ONa
(3) Formation of intermolecular H-bonding
CHCl2 CHCl2 (4) van der Waals force of attraction
(1) (2)
58. The product formed when propanal undergoes aldol
condensation
O
ONa ONa
(1) CH3 — C == CH — CH2 — C — H
CHO COOH
(3) (4)
CH3
O
53. Major product f ormed by the dehydration of (2) CH3 — CH2 — CH == C — C — H
CH3
in acidic medium is OH O
OH
(3) CH3 — CH2 — CH — CH — C — H
CH3
(1) (2) OH O
(4) CH3 — CH — CH — C — H
CH3
59. Most acidic compound among the following is
(3) (4)
(1) NO2CH2 COOH (2) CH2 == CHCOOH
(3) HCOOH (4) CF3COOH
HI(conc.)
54. CH3OCH2CH3 A + B. Product A and B 60. Which of the following is not a barbiturate?
Excess,
(1) Veronal (2) Morphine
are (3) Amytal (4) Luminal
(1) CH3OH + CH3CH2I (2) CH3I + CH3CH2 OH 61. Optical isomerism is shown by
(3) CH3OH + CH3CH2OH(4) CH3I + CH3CH2I (1) Lactic acid (2) Malonic acid
(3) Succinic acid (4) Oxalic acid
(i) RMgBr
55. A
(ii) H O
2 Alcohol 62. Select the incorrect statement regarding
3
acetophenone.
A can be
(1) Forms 2,4-DNP derivative
(1) HCHO (2) RCHO (2) Gives yellow precipitate on heating with iodine in
(3) RCOR (4) RCOOR the presence of sodium hydroxide
(3) Does not reduce Fehling’s reagent
56. In the reaction
(4) Give positive Tollen’s test
OH ONa OH 63. Acetone is treated with excess of ethylene glycol in
COOH the presence of hydrogen chloride. The product
NaOH (i) CO2 obtained is
+
(ii) H
O CH2 O CH2
CH3
The electrophile involved is (1) C (2) CH2
CH3 O CH2 O CH2
(1) CO2 (2) CO
SPACE FOR ROUGH WORK CH3
(3) H+ (4) C OOH O CH O CH2
57. The boiling points of aldehydes and ketones are (3) CH2 (4) CH3 — CH
higher than corresponding hydrocarbons and ethers O CH O CH2
of comparable molecular masses. It is due to CH3
6/16
ALLEN
64. Nucleophilic addition reaction will be most favoured 70. For the reaction
in NH2
(1) CH3CH2COCH3 (2) CH3COCH3
HNO3
(3) CH3CHO (4) HCHO H2SO4, 288 K
65. Reaction by which benzoic acid cannot be prepared NH2 NH2 NH2
is
NO2
CH3 CH2 — CH3 + +
– –
(i) KMnO4/OH (i) KMnO4 /OH NO2
(1) +
(ii) H
(2) +
(ii) H
NO2
CH3
The correct increasing order of the amount of
(3) CH3 — CH — CH3 (4) CH3 — C — CH3 products formed is
– –
(i) KMnO4/OH (i) KMnO4 /OH
+
(ii) H
+
(ii) H (1) Ortho < Para < Meta
66. In the following sequence of reaction (2) Meta < Ortho < Para
KCN
CH3Br H3 O
A PCl5
B H2
C D (3) Meta < Para < Ortho
Pd-BaSO4
The end product (D) is (4) Ortho < Meta < Para
(1) CH3CH2OH (2) CH3CHO 71. Which of the following reaction is appropriate for
(3) CH3CH3 (4) CH3OCH3 converting primary amine to isocyanide?
67. Wolff Kishner reduction of ketone is carried out in the (1) Carbylamine reaction
presence of
(1) H2/Pt (2) Hoffmann bromamide reaction
(2) (i) NH2 – NH2, (ii) KOH/Ethylene glycol/ (3) Stephen’s reaction
(3) (i) Zn – Hg/HCl, (ii) H2O/H+ (4) Gattermann reaction
(4) Li AlH4
72. W hich of the f ollowing amines will not f orm
O
18 H2O/H
+ sulphonamides with benzene sulphonyl chloride?
68. CH3 CH2 CH2 — C — O — C2H5 A + B.
A and B are CH3
18
(1) CH3 CH2CH2COOH C2H5OH (1) CH3NH2 (2) CH3 — C — NH2
18 CH3
(2) CH3CH2CH2COOH C 2H5 OH
18 18
(3) CH3 CH2CH2COOH C 2H5 OH (3) CH3 — NH — CH3 (4) CH 3 — N — CH 3
(4) CH3 CH2CH2COOH C2H5 OH CH3
69. The correct increasing order of basic strength for the
following compounds is 73. Benzene diazonium chloride on reaction with phenol
in alkaline medium forms
CH3 CH3
N NH — CH3 NH2
(1) p-Hydroxyazobenzene (Blue dye)
7/16
ALLEN
CONH2 CH2NH2 CN n
(1) (2)
O O
NH2 COONa (4) — OCH2 — CH2 — O — C — —C—
(3) (4) n
8/16
ALLEN
86. Deficiency of which vitamin causes pernicious 89. Which of the following is not a broad spectrum
anaemia? antibiotics?
(1) Vitamin B1 (2) Vitamin B6 (1) Ampicillin (2) Chloramphenicol
(3) Vitamin B12 (4) Vitamin K (3) Vancomycin (4) Penicillin G
87. Which among the following is a purine base? 90. Which of the following does not act as a food
(1) Uracil (2) Guanine preservative?
(3) Thymine (4) Cytosine (1) Table salt
88. Essential amino acid among the following is (2) Sugar
(1) Alanine (2) Methionine (3) Blue vitriol
(3) Tyrosine (4) Glutamine (4) Vegetable oil
[ BIOLOGY ]
100. Which one is the most important cause that leads 104. How many statement(s) is/are correct regarding
to extinction of plants and animals? CNG?
(1) Alien species invasions
a. CNG is cheaper than petrol or diesel
(2) Over-exploitation
b. CNG burns less efficiently, unlike petrol or
(3) Co-extinction diesel.
(4) Habitat loss and fragmentation
c. It can neither be siphoned off by thieves nor
101. Given below pie chart is representation of global adulterated like petrol or diesel.
biodiversity of v ertebrates. In this pie chart
A, B and C respectively represent d. It is compressed natural gas.
(1) 2 (2) 3
(3) 4 (4) 1
A
C 105. When two closely related species competing for the
same resource, cannot co-exist indefinitely and the
competitively inferior one is eventually eliminated,
then the interaction is known as
(1) Competition release
(2) Interference competition
B
(3) Competitive exclusion
A B C
(4) Camouflage
(1) Fishes Amphibians Birds
106. Integral form of the exponential growth equation is
(2) Birds Reptiles Mammals
(3) Mammals Reptiles Amphibians dN
(1) = rN (2) Nt = N0e rt
(4) Reptiles Amphibians Birds dt
102. Which one is incorrect match?
(1) Joint Forest dN dN N
(3) = (b – d) × N (4) = rN 1 –
Management – Started in 1980, in India dt dt K
(2) Good ozone – Troposphere
107. “Mammals from colder climates generally have
(3) Snow-blindness – UV-B shorter ears and limbs to minimise heat loss”. This
(4) Montreal is called
Protocol – Signed in Canada (1987)
(1) Allen’s rule
103. Match the column I with column II and choose the
correct option. (2) Jordan’s rule
Column I Column II (3) Bergman’s rule
a. BOD (i) Severe respiratory (4) Rensch’s rule
problems
108. Match the column I with the column II and choose
b. DDT (ii) Unit is decibel the correct option.
c. SO2 (iii) Related to amount of Column I Column II
organic waste in
water (Age Pyramid) (Population growth)
d. Noise (iv) Primary pollutant. a. Urn shaped (i) Growing population
(1) a(i), b(iv), c(iii), d(ii) SPACE FOR ROUGH WORK
b. Triangular (ii) Stable population
(2) a(iii), b(iv), c(i), d(ii) c. Bell shaped (iii) Declining population
(3) a(iv), b(iii), c(i), d(ii) (1) a(i), b(ii), c(iii) (2) a(ii), b(i), c(iii)
(4) a(iii), b(iv), c(ii), d(i) (3) a(iii), b(ii), c(i) (4) a(iii), b(i), c(ii)
10/16
ALLEN
109. Which one is not a key functional aspect of an 115. Read the following statements and select the correct
ecosystem? one (s).
(1) Decomposition (2) Energy flow a. A trophic level represents a functional level not
(3) Species composition (4) Nutrient cycle a species as such.
110. Identify true (T) or false (F) statements and select the b. A given species may occupy more than one
correct option. trophic levels in the same ecosystem at the
a. Tropical rainforest and sugarcane field are the same time.
most productive ecosystems.
c. Energy at a lower trophic level is always less
b. Secondary productivity is the rate of formation of than at a higher level
new organic matter by consumers.
(1) Only a
c. Humus is colourless and partly decomposed
organic matter. (2) Only a and b
a b c (3) Only b and c
(1) T T F (4) All a, b and c
(2) T T T 116. Match the column I with column II and choose the
(3) F T F correct option.
(4) T F F Column I Column II
111. Select the true statement(s) for reducers. a. Earth summit (i) Direct economic
benefits from nature
a. They are natural scavengers.
b. World summit on (ii) Rio de Janeiro (1992)
b. Replenish the soil naturally with minerals sustainable
c. They are top carnivores development
(1) Only a (2) Only a and b c. Narrowly utilitarian (iii) Replenishment of
atmospheric O2 by
(3) Only b and c (4) All a, b and c
plants
112. What is incorrect about primary succession?
d. Broadly utilitarian (iv) Johannesburg (2002)
(1) It starts at barren area (1) a(iv), b(ii), c(i), d(iii) (2) a(ii), b(iv), c(i), d(iii)
(2) Climax reaches very quickly (3) a(iv), b(ii), c(iii), d(i) (4) a(ii), b(iv), c(iii), d(i)
(3) Pioneer community does not get established 117. How many hotspots are present in India?
easily (1) One (2) Three
(4) Cooled volcanic lava, igneous rocks etc. are (3) Two (4) Four
areas where it starts 118. Which one of the following is the largest tiger
113. Hydrarch succession proceeds from reserve?
(1) Corbett National Park
(1) Hydric to xeric conditions
(2) Kanha National Park
(2) Xeric to hydric conditions
(3) Bandipur National Park
(3) Mesic to hydric conditions
(4) Nagarjunsagar Sirisailam
(4) Hydric to mesic conditions
119. Van Mahotsav is being carried out in India since
114. Which one is odd w.r.t. gaseous cycle? 1950. Under this movement, government and private
agencies perform the plantation every year, during
SPACE FOR ROUGH WORK
(1) Carbon cycle
(1) January and March
(2) Hydrogen cycle
(2) July and February
(3) Phosphorus cycle
(3) April and July
(4) Nitrogen cycle (4) October and December
11/16
ALLEN
120. Government of India has passed Water (Prevention 127. Which one does not play role in determining hot
and Control of Pollution) Act, in spot?
(1) 1986 (2) 2002 (1) Very high levels of species richness
(3) 1990 (4) 1974 (2) High degree of endemism
121. Select the mismatched pair. (3) Low genetic diversity
(3) Motor vehicles equipped with catalytic converter, b. Pyramid of biomass of an aquatic ecosystem
should use leaded petrol may be inverted.
(4) Euro III norms stipulate that sulphur should be c. Least productive ecosystem is desert and deep
controlled at 350 ppm in diesel sea.
126. Two major green house gases w.r.t. their global a b c
warming potential are
SPACE FOR ROUGH WORK
(1) F F F
(1) CH4 and N2O
(2) F T T
(2) CFCs and N2O
(3) CO and CFCs (3) T T T
2
(4) CO2 and CH4 (4) T F T
12/16
ALLEN
133. Characteristics of man-made ecosystems are (1) Statement A is correct but statement B is
a. Huge cycling of nutrients incorrect
b. Simple food chain (2) Statement A is incorrect but statement B is
c. Little diversity correct
(1) Only a and b (2) Only b and c (4) Both statements are correct
(3) Only c and d (4) Only a and d 140. Select the correct option w.r.t. fresh water fishes.
134. For ecosystem, term ‘biocoenosis’ was used by (1) Catla, Rohu and Hilsa
(1) Forbes (2) A.G. Tansley (2) Sardines, Mackerel and Pomfrets
(3) C. Mobius (4) Friederich (3) Rohu, Pomfrets and Catla
(3) Poultry farming – Ducks (1) a(i), b(ii), c(iii), d(iv) (2) a(iv), b(iii), c(ii), d(i)
(4) Pisciculture – Shellfish (3) a(ii), b(iii), c(i), d(iv) (4) a(iii), b(iv), c(i), d(ii)
137. The breed Karan Swiss has been developed by 142. Hisardale is a resultant of crossbreeding between
crossing indigenous Sahiwal cows with exotic Brown (1) Holstein-Friesian and Sahiwal
Swiss bulls at (2) Holstein - Friesian and Tharparkar
(1) ICAR (3) Bikaneri ewes and Merino rams
(2) NDRI (4) Male donkey and female horse
(3) CDRI 143. Enhancement in agricultural yield by efforts of
Norman Borlaug and others led to
(4) GEAC
(1) White revolution (2) Green revolution
138. Choose the incorrect statement w.r.t. MOET.
(3) Blue revolution (4) Operation flood
(1) Hormones with FSH like activity are administered
144. It is estimated that more than ___ percent of the
to induce follicular maturation and super ovulation
world livestock population is in India and China.
(2) The superior cow is either mated with an elite
Select the option which fills the blank correctly.
bull or artificially inseminated
(1) 25% (2) 50%
(3) This technology has been demonstrated for
(3) 70% (4) 90%
cattles, rabbits, sheeps, goats and chicken
145. Choose the incorrect statement w.r.t. inbreeding.
(4) The fertilized eggs at 8-32 celled stage are
transferred to surrogate mothers (1) Increases homozygosity
139. Read the following statements A and B. Choose the (2) Exposes harmf ul recessive traits that are
SPACE FOR ROUGH WORK eliminated by selection process
correct option.
(3) Refers to the mating of the unrelated individuals
Statement A: Leghorn is exotic variety of poultry
within the same breed for 4-6 generations
birds categorised as a ‘Layer’.
(4) Is necessary if we want to evolve pureline in an
Statement B: Ranikhet is viral disease of poultry. animal breed
13/16
ALLEN
146. Breeds of cattle and chicken are respectively (1) Lysozyme and cellulase
(1) Brown swiss and Marwari (2) Ribonuclease and protease
(2) Jersey and Leghorn (3) Chitinase and lipase
(3) Rhode island Red and Ayrshire (4) Protease and deoxyribonuclease
(4) Hisardale and Mule 153. Select the incorrect statement w.r.t. gel
electrophoresis.
147. ‘The integration of natural science and organisms,
cells, parts thereof and molecular analogues for (1) DNA fragments can be separated by forcing
products and services.’ them to move towards the ‘cathode’ under an
electric field through a medium
The above definition of biotechnology was given by
(2) Commonly used matrix for gel electrophoresis of
(1) Genetic Engineering Approval Committee
DNA is agarose extracted from sea weeds
(2) European Federation of Biotechnology
(3) The DNA fragments separate according to their
(3) US Patent and Trademark Office size through sieving effect provided by the
(4) International Union of Biochemistry agarose gel
148. The linking of antibiotic resistance gene with the (4) We cannot see pure stained DNA fragments
plasmid vector became possible through the help of even in visible light
(1) Restriction endonuclease 154. Intercalating dye ethidium bromide, stacks between
base pairs of DNA and stains it. Upon exposure to
(2) DNA ligase UV light DNA appears
(3) DNA polymerase (1) Dull blue (2) Bright green
(4) Exonuclease (3) Bright orange (4) Yellow
149. The antibiotic resistance gene isolated by Cohen and 155. Select the first restriction endonuclease, isolated and
Boyer was inserted in plasmid native to which characterised from Haemophilus influenzae
organism?
(1) Eco RI (2) Hind I
(1) Salmonella typhimurium
(3) Hae I (4) Hind II
(2) Bacillus amyloliquefaciens
156. If a ‘4 kb’ fragment of DNA is to be amplified from
(3) Escherichia coli 8 kb plasmid then, how many copies are obtained at
(4) Bacillus thuringiensis the end of 30 PCR cycles approximately?
150. Plasmid DNA cannot (1) 1 billion (2) 1 million
(1) Be double stranded (3) 2 billion (4) 2 million
(2) Be coated with histone proteins 157. Part of the plasmid pBR322 that controls copy
number of foreign DNA linked to it is
(3) Replicate independent of chromosomal DNA
(1) Rop (2) Multiple cloning sites
(4) Circular in shape
(3) Selectable marker (4) Origin of replication
151. In gel electrophoresis, the separated band of DNA
containing the gene of interest is cut and extracted 158. A single PCR reaction involves three temperature
from the gel piece. This step is known as dependent steps. Their correct sequence is
(1) Denaturation (1) Annealing Denaturation Amplification
(2) Spooling (2) Denaturation Annealing Extension
152. While isolation of DNA from tomato, RNA can be 159. Treatment with salts of which ion facilitates efficiency
removed by the treatment with ____ whereas of transfer of foreign DNA into host cells?
proteins can be removed by treatment with ______. (1) O2– (2) Ca2+
Select the option which fills the blanks correctly. (3) K+ (4) Na+
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160. Recombinant DNA is directly injected into the 166. Transgenic plants have been useful in all except
nucleus of an animal cell. This method is known as (1) Increased reliance on chemical pesticides
(1) Transformation (2) Microinjection (2) Made crops more tolerant to abiotic stresses
(3) Biolistic (4) Electroporation (3) Increased efficiency of mineral usage by plants
161. Select the odd one w.r.t. direct gene transfer in plant (4) Helped to reduce post-harvest losses
protoplasts.
167. Select the incorrect statement w.r.t. Golden rice.
(1) Electroporation (2) Disarmed Ti plasmid
(1) It is a transgenic variety of wild type rice
(3) Microinjection (4) Particle gun
(2) Grains of the rice are red in colour due to
162. Downstream processing of desired product does not -carotene
involve
(3) Gene for -carotene has been taken from the
(1) Isolation of product flower Daffodils
(2) Purification of expressed protein (4) Gene for synthesis of -carotene was inserted
(3) Marketing of extracted product into the genome of wildtype rice using
(4) Expression of foreign gene Agrobacterium tumefaciens.
163. Choose the option that represents an incorrect 168. W hich gene encodes the protein to control
statement w.r.t. bioreactors. lepidopteran corn borer’s infection in corn plants?
(1) Also known as fermenters for growth of microbes (1) cry IAb
under a controlled environment (2) cry IAc
(2) Large volumes i.e. 100 – 1000 L of culture can (3) cry IIAb
be processed (4) cry IIAd
(3) Anti-foaming agents are required to increase the 169. A nematode named_____infects the roots of tobacco
foaming caused by presence of proteins in the plants and causes a great reduction in its yield.
culture medium
Select the option which fills the blank correctly.
(4) Bubbles dramatically increase the oxygen
transfer area (1) Meloidogyne incognita
164. Read the following statements and choose the (2) Bacillus thuringiensis
correct option. (3) Ascaris lumbricoides
Statement A: In batch fermentation, the conditions (4) Escherichia coli
once set are not changed from outside after the 170. Select the mismatch.
process of fermentation starts.
(1) Rosie – Transgenic cow
Statement B: Batch fermentation is called an open
(2) Emphysema – -1- antitrypsin
culture system.
(3) Dolly – Transgenic sheep
(1) Statement A is correct
(4) ANDi – Transgenic monkey
(2) Statement B is correct
171. Select the correct statement w.r.t. SCID.
(3) Statement B is correct explanation of A
(1) Is caused due to absence of gene for adenosine
(4) Both statements are correct
deaminase
165. In simple stirred tank type of bioreactor, stirrer is
(2) Cannot be treated by bone marrow
responsible for
SPACE FOR ROUGH WORK transplantation at early stage
(1) Increase in the pH
(3) In presence of adenosine deaminase,
(2) Decrease in temperature lymphocyte proliferation is inhibited
(3) Extraction of product (4) Without RBCs, ADA deficient children are wide
(4) Uniform availability of oxygen open to attacks of viruses and bacteria
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172. The main challenge for production of insulin using 176. Choose the organism, whose natural product is used
rDNA techniques was getting insulin assembled in as a clot buster for removing thrombus
mature form by (1) Entamoeba coli
(1) Obtaining correct sequence of amino acids in (2) Streptococcus
chain A and B
(3) Methanococcus
(2) Removal of chain ‘A’ from proinsulin
(4) Vibrio cholerae
(3) Formation of disulfide bonds between correct
amino acids 177. Which of the following enzyme is thermally stable?
(4) Transformation of E.coli with cDNA of chain (1) Pancreatic lipase (2) Taq polymerase
A and B. (3) Gastric protease (4) E.coRI
173. The term, used to refer to the use of bio-resources 178. Which of the following is a source of energy during
by multinational companies and other organisation a PCR?
without proper authorisation from the countries and
people concerned without compensatory payment is (1) ATP (2) dNTPs
(1) Biopatent (2) Bioethics (3) DNA polymerase (4) Thermal cycler
(3) Bioweapon (4) Biopiracy 179. There is a restriction endonuclease called EcoRI.
What does alphabet ‘R’ in it stand for?
174. Choose the odd one w.r.t. technique used for early
detection of diseases. (1) Genus
(3) Urine analysis (4) Order in which the enzyme was isolated from the
source bacteria
(4) Polymerase chain reaction
180. Insertion of gene of interest in pBR322 at which
175. How many recombinant therapeutic are currently restriction enzyme site results in susceptiblility to
being marketed in India? ampicillin in recombinant E.coli?
(1) 10 (2) 12 (1) Pvu II (2) Pst I
(3) 15 (4) 30 (3) BamHI (4) Cla I
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MOCK TEST
ANSWERS
1. (2) 37. (4) 73. (2) 109. (3) 145. (3)
2. (3) 38. (2) 74. (3) 110. (1) 146. (2)
3. (2) 39. (2) 75. (3) 111. (2) 147. (2)
4. (3) 40. (3) 76. (2) 112. (2) 148. (2)
5. (2) 41. (2) 77. (3) 113. (4) 149. (1)
6. (1) 42. (3) 78. (2) 114. (3) 150. (2)
7. (3) 43. (4) 79. (1) 115. (2) 151. (4)
8. (3) 44. (4) 80. (3) 116. (2) 152. (2)
9. (2) 45. (2) 81. (2) 117. (2) 153. (1)
10. (2) 46. (3) 82. (4) 118. (4) 154. (3)
11. (2) 47. (1) 83. (4) 119. (2) 155. (4)
12. (1) 48. (4) 84. (3) 120. (4) 156. (1)
13. (2) 49. (3) 85. (2) 121. (3) 157. (4)
14. (3) 50. (4) 86. (3) 122. (1) 158. (2)
15. (4) 51. (3) 87. (2) 123. (2) 159. (2)
16. (1) 52. (2) 88. (2) 124. (2) 160. (2)
17. (3) 53. (3) 89. (4) 125. (3) 161. (2)
18. (3) 54. (4) 90. (3) 126. (4) 162. (4)
19. (4) 55. (2) 91. (2) 127. (3) 163. (3)
20. (1) 56. (1) 92. (1) 128. (2) 164. (1)
21. (4) 57. (2) 93. (3) 129. (4) 165. (4)
22. (3) 58. (2) 94. (4) 130. (1) 166. (1)
23. (1) 59. (4) 95. (3) 131. (3) 167. (2)
24. (2) 60. (2) 96. (1) 132. (3) 168. (1)
25. (1) 61. (1) 97. (4) 133. (2) 169. (1)
26. (4) 62. (4) 98. (4) 134. (3) 170. (3)
27. (4) 63. (1) 99. (3) 135. (4) 171. (1)
28. (2) 64. (4) 100. (4) 136. (3) 172. (3)
29. (1) 65. (4) 101. (1) 137. (2) 173. (4)
30. (2) 66. (2) 102. (2) 138. (3) 174. (3)
31. (3) 67. (2) 103. (2) 139. (4) 175. (2)
32. (2) 68. (2) 104. (2) 140. (4) 176. (2)
33. (1) 69. (1) 105. (3) 141. (2) 177. (2)
34. (4) 70. (4) 106. (2) 142. (3) 178. (2)
SPACE FOR ROUGH WORK
35. (2) 71. (1) 107. (1) 143. (2) 179. (3)
36. (3) 72. (4) 108. (4) 144. (3) 180. (2)
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PHYSICS sin30 1
sin e 3
1. Answer (2)
360 3
Hint: N sin e
. 2
Sol.: If N is even, number of image = N – 1. e 60
If N is odd and object is placed symmetrical, number i+e=A+
of image = N – 1. 60 + 60 = 60 +
If N is odd and object is placed unsymmetrical, 60
number of image = N. 4. Answer (3)
For = 60° Hint: Length of the telescope for near point
N=6 adjustment (at near point, eye is most strained) is
Number of image = 6 – 1 = 5.
2. Answer (3) Dfe
L f0 .
1 1 1 D fe
Hint: Use . fe D 4 25
v u f Sol.: L f0 50 53 cm .
v I fe D 4 25
Sol.: m – 5. Answer (2)
u O
v 1
– 2 v –2u Hint: ic = sin 1 .
u 2
v = +10 cm
c
1 1 1 Sol.: a t1
1
v u f
ct1
1 1 1 1
– a
10 5 f
ct 2
1 1 2
– b
10 f
f = –10 cm
ic sin–1 1
R = 2f 2
R –20 cm bt
i c sin–1 1
3. Answer (2) at 2
Hint: Use Snell’s law and prism formula. 6. Answer (1)
Sol.:
60° 1 1 1
Hint: ( – 1) – .
f R1 R2
60°
Sol.:
R
4 cm
sin i
3 d
sin r1 R–d
sin 60 1
3 sin r1
sin r1 2 (R – d ) 2 + (4)2 = R2
r1 30 d 2 – 2Rd + 16 = 0
r1 + r2 = A 16
SPACE FOR ROUGH WORK
R (neglecting d2)
r2 = 30° 2d
sin r2 1 16
R = 20 cm
sin e 3 2 4 10–1
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a2 Y A B OR Gate .
Hint: ZF .
21. Answer (4)
Hint and Sol.: For forward biasing of diode potential
a2
Sol.: ZF of p-type should be greater than potential of n-type
–3 V < –2 V
(4 10 –3 )2 Only diode in option (4) is reversed biased.
Z = 20 m 22. Answer (3)
800 10 –9
Hint: Vdiode = VR(3 )
17. Answer (3)
Hint: Fringe shift due to introduction of mica plate in Sol.: I 7
( – 1) t D
front of one slit = .
d 6.6 V 3 0.3 V
4D
Sol.: Position of fourth bright = .
d
According to given condition P.d. across 3 is 0.3 V
6.6 = 0.3 + 7I
( – 1) t D 4D 6.3 = 7I
d d
I 0.9 A
4 SPACE FOR ROUGH WORK (1)
23. Answer
t
–1 Hint: If diode is in forward biased condition. Then it
18. Answer (3) act as short. If diode is in reverse bias condition. It
Hint: Concept of Snell’s law and Brewster's law. will act as open.
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h 1 1 1
1 R 2 – 2
.
P1 max (1) (2)
h 4
2 max .
P2 3R
1 P2 For minimum wavelength min
2 P1 n1 = 1, n2 =
1 1
2 R
2 min
1 1
2 . min
2 R
35. Answer (2) max 4 4
min 3R 1 3
h
Hint: . R
P 39. Answer (2)
Sol.: P 2e mV Hint and Sol.:
1 V2 n(n – 1) 5(5 – 1)
No. of spectral lines 10 .
2 V1 2 2
40. Answer (3)
1
4 Z2
2 Hint: N
10 – 9 sin4
2 2
4
2 2.5 Å . sin4 2
Sol.: N1 2
36. Answer (3) N2
Hint: = 0 + KE sin4 1
2
1
Sol.: KE1 mV 2 N1 sin4 45
2
N2 sin4 30
1
KEf 9 mV 2 9 KEi N1
2 4
i = h= N2
f = 2h = 2 N1 20
= 0 + KE N2 5.
4 4
2 = 0 + 9KE 41. Answer (2)
2= 0 + 9 – 90 Hint and sol.: Density of nucleus is independent of
7 = 80 mass number
7h 1 1
0 .
8 2 1
37. Answer (4) 42. Answer (3)
Hint: PE = 2TE Hint: Apply conservation of linear momentum.
Sol.: Pf = Pi
13.6 eV
Sol.: TE – –3.4 eV 4v 0 (220 – 4)v 0.
(2)2
– 4v 0 4v 0 v 0
PE – 6.8 eV . v v
216 216 54
38. Answer (2)
43. Answer (4)
1 1 1 SPACE FOR ROUGH Hint:
WORKN N0 .
Hint: R 2 – 2 .
n
1 n2 2n
Sol.: For maximum wavelength max N N
Sol.: 0 n0 .
n1 = 1, n2 = 2 8 2
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C — Br – Br
C+ acidic strength of phenol.
Sol.: Picric acid is the strongest acid due to three
electron withdrawing group (i.e. –NO2) at ortho and
para positions.
Resonance stabilised OH
47. Answer (1) O2N NO2
Hint: Alc KOH shows dehydrohalogenation reactions.
Sol.:
Br CH3 NO2
–+
C2H5OK 51. Answer (3)
CH3 — CH — CH — CH2 — CH3
C2H5OH Hint: B 2 H 6 f ollowed by H 2O 2 /OH – shows anti
CH3 Markovnikov addition.
Sol.:
CH3 — CH == C — CH2 — CH3
(1) B 2H 6
CH3 — CH == CH2 – CH3 CH2 CH2 OH
Major (Saytzeff product) (2) H2 O2 /OH
PCl5 (A)
Na
+
SPACE FOR ROUGH WORK CH3CH2CH2Cl CH3CH2CH2 ONa +
1
H
CH3 2 2
(C) (B)
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(i) RMgBr O O
RCHO
(ii) H O
R 2CHOH 2 Alcohol
3
Sol.: CH3 — CH2 — C — CH3 < CH3 — C — CH3
(i) RMgBr
RCOR
(ii) H O
R3 COH 3 Alcohol
3
O O
(i) RMgBr
RCOOR R3 C OH 3 Alcohol
(ii) H3O
< CH3 — C — H < H — C — H
56. Answer (1) (Order of rate of nucleophilic addition)
SPACE
Hint: CO2 is the electrophile in the given FOR ROUGH
reaction. WORK (4)
65. Answer
57. Answer (2) Hint: Alkyl benzene containing benzylic H-atom on
Hint: Aldehydes and ketones are more polar than vigorous oxidation with acid or alkaline KMnO4 gives
corresponding ethers or hydrocarbons. aromatic carboxylic acids.
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NH2
CH3 — CH — CH3 COOH
–
(i) KMnO4/OH pKb = 9.38
+
(ii) H
(III)
Basic strength I > II > III
CH3 70. Answer (4)
CH3 — C — CH3 Hint: Anilinium ion is meta directing.
NH 2
–
(i) KMnO4/OH
No reaction. HNO 3 , H 2SO 4, 288 K
(ii) H
+
Sol.:
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Narrowly – Direct economic benefits Sol.: – In designated priority area, there should be
utilitarian from nature very high degree of species richness, high
Broadly – Replenishment of degree of endemism and degree of threat in
utilitarian atmospheric O2 by plants terms of habitat loss.
117. Answer (2) – Low genetic diversity is not a criteria for
Sol.: Three hotspots are present in India. These are hotspot determination.
(i) Western Ghats and Sri Lanka.
128. Answer (2)
(ii) Indo-Burma.
Sol.: Sacred groves are in-situ conservation strategy
(iii) Himalaya.
while others are cause of biodiversity loss (Evil
118. Answer (4)
Quartet).
Hint: This tiger reserve is situated at Guntur, Andhra
Pradesh (India). 129. Answer (4)
Sol.: The largest tiger reserve is Nagarjunsagar Sol.: In a region, biodiversity loss may lead to
Srisailam. decline in productivity, reduced resistance to
119. Answer (2) environmental perturbation like drought and increased
Sol.: Van Mahotosava is being carried out every variability of ecosystem processes.
year in India, during July and February months.
120. Answer (4) 130. Answer (1)
Sol.: Government of India has passed Water Act in Hint: A part of incident solar radiation get reflected
1974. by clouds, scattered by dust particles etc.
121. Answer (3)
Sol.: Thus, PAR cannot be 100% of incident solar
Hint: Cadmium causes painful skeletal deformities.
radiation. It is < 50% of incident solar radiation.
Sol.: – These painful skeletal deformities are called
Itai-itai disease. 131. Answer (3)
– Arsenic causes blackfoot disease. Hint.: Herbivores are primary consumers.
122. Answer (1) Sol.:
Sol.: Photochemical smog mainly has O3, PAN &
NOx. Solar radiation Producers Herbivores 1 Carnivores
(1 Consumer) (2° consumer)
123. Answer (2)
Hint: These trees absorb sound vibrations to a great 132. Answer (3)
extent.
Sol.: Detritus is dead remains of leaves, bark,
Sol.: These are neem & ashoka.
flowers and animals including faecal matter.
124. Answer (2)
Hint: Bhopal gas tragedy occurred on 2nd Dec. 1984. 133. Answer (2)
Sol.: It was due to leakage of poisonous gas Methyl Hint: In man-made ecosystems, productivity is high.
isocyanate (MIC) from pesticide plant of Union
Sol.: – In this ecosystem, little cycling of nutrients
Carbide factory.
occur.
125. Answer (3)
Sol.: Motor vehicles equipped with catalytic converter – Simple food chain is also found.
should use unleaded petrol because lead in the 134. Answer (3)
petrol inactivates the catalyst.
Hint: For ecosystem, term ‘biocoenosis’ was used
126. Answer (4)
by C. Mobius.
Sol.: CO2 and CH4 are commonly known as green-
house gases. 135. Answer (4)
CO2 – 60% contribution for greenhouse effect
SPACEeffect
CH4 – 20% contribution for greenhouse FOR ROUGH WORK dN
Hint: Equation = rN describes geometric growth.
127. Answer (3) dt
Hint: Concept of hotspot was developed to designate
priority areas for in-situ conservation. Sol.: Geometric growth results J-shaped curve.
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151. Answer (4) single stranded template. During extension, the DNA
Hint: Process of extracting one material from polymerase adds nucleotides to the primer,
another. synthesizing a new DNA strand.
Sol.: Spooling is the process by which precipitated 159. Answer (2)
DNA can be removed from suspension. Denaturation Hint: Identify a divalent cation.
is separation of DNA strands at high temperatures.
Sol.: Possibly, CaCl2 causes the DNA to precipitate
152. Answer (2) onto the surface of the host cells, thereby enhancing
Hint: Enzymes are named after the substrates they chances of entry of rDNA into host cell at 42°C.
act upon. 160. Answer (2)
Sol.: Lysozyme, chitinase and cellulase act on
Hint: Identify the method applicable for animal cells.
bacterial, fungal and plant cell walls respectively.
Sol.: Transformation is a procedure through which a
153. Answer (1)
piece of DNA is introduced into a host bacterium.
Hint: DNA is a negatively charged molecule.
In biolistic/gene gun method, cells are bombarded
Sol.: DNA can be separated by forcing them to
with high velocity microparticles of gold or tungsten
move towards the positive electrode named anode.
coated with DNA.
The separated DNA fragments can be visualized
only after staining the DNA with ethidium bromide In electroporation, short electrical impulses of high
followed by exposure of UV rays. field strength are given to the target host cells.
154. Answer (3) 161. Answer (2)
Hint: Colour depends on type of dye used for Hint: Disarmed Ti plasmid is a vector.
staining. Sol.: Disarmed Ti plasmid of Agrobacterium
Sol.: We can see bright orange coloured bands of tumefaciens is a method of indirect gene transfer.
DNA fragments when stained with ethidium bromide 162. Answer (4)
upon exposed to UV rays. Bands appear bright Hint: This involves translation of desired mRNA.
orange pink in color.
Sol.: Isolation, purification, formulation, packaging,
155. Answer (4) storage and marketing of product are parts of
Hint: Identify a type II restriction enzyme. downstream processing.
Sol.: Hind II always cuts DNA molecules at a 163. Answer (3)
particular position by recognising a specif ic Hint: These structures are attached alongside
sequence of six base pairs. impellers in a bioreactor.
156. Answer (1) Sol.: Anti-foaming agents are required to reduce the
Hint: Plasmid is a ds DNA molecule. foaming caused by proteins or some molecules
Sol.: In PCR, it is possible to generate ‘2n’ molecules produced by the microbes.
after ‘n’ number of cycles. i.e. 230 ~ 1 billion 164. Answer (1)
157. Answer (4) Hint: This culture contains initial limited amount of
Hint: This region lies close to region coding for nutrients.
proteins involved in replication of plasmid. Sol.: In continuous culture system, the used
medium is drained out from one side, while fresh
Sol.: The nucleotide sequence of ‘Ori’ is also
medium is added from other side to maintain the
responsible for controlling the copy number of the
cells in their physiologically most active log/
plasmid and linked DNA. So, if we want to obtain
exponential phase.
multiple copies of desired DNA, it should be cloned
in a vector whose origin supports high copy number. Batch culture system is a closed type culture while
continuous culture system is open type.
158. Answer (2)
165. Answer (4)
Hint: The first of step of PCR requires 94°C for
separation of DNA strands. Hint: The agitator facilitates even mixing of nutrients.
Sol.: The stirrer facilitates proper mixing and oxygen
Sol.: In denaturation, the high temperature SPACE FOR ROUGH WORK
breaks
the hydrogen bonds present between the two strands availability throughout the bioreactor.
of the template DNA. During annealing, the primers 166. Answer (1)
bind to their complementary sequences on the Hint: Pest-resistant crops.
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Sol.: Genetic modifications have reduced reliance on Sol.: Bioethics may be viewed as a set of standards
chemical pesticides. that may be used to regulate our activities in relation
167. Answer (2) to biological world. Patents which are being granted
for biological entities and for products derived from
Hint: Colour of -carotene. them, are known as biopatents.
Sol.: The grains of rice are yellow in colour due to 174. Answer (3)
-carotene. The rice is commonly called golden rice
Hint: Identify the conventional method of diagnosis.
due to its appearance. -carotene is precursor of
vitamin A. Sol.: Modern methods of diagnosis serve purpose of
early diagnosis which are RDT, PCR and ELISA.
168. Answer (1)
175. Answer (2)
Hint: Gene in maize that affects corn borer.
Hint: A dozen of recombinant therapeutics are
Sol.: The protein encoded by gene cryI Ac and permitted to be marketed in India.
cryIIAb controls cotton bollworm while, that of
Sol.: At present, about 30 recombinant therapeutics
cryI Ab controls corn borer.
have been approved globally and 12 of these are
169. Answer (1) presently being marketed in India.
Hint: Identify an Aschelminth. 176. Answer (2)
Sol.: M. incognita attacks tobacco plant. It attacks Hint: Organism which produces streptokinase.
the roots cells causing roots to grow into large galls/ Sol.: Streptokinase produced by Streptococcus
knots, damaging the crop and reducing its yield. bacterium and modified by genetic engineering is
Hence, called root-knot nematode. used as a ‘Clot buster’ for removing clots from blood
170. Answer (3) vessels of patients who have undergone myocardial
Hint: Select a cloned animal. infarction.
Sol.: Dolly is not a transgenic animal. 177. Answer (2)
ANDi was the first genetically modified monkey. Hint: Enzyme obtained from bacterium Thermus
aquaticus.
-1- antitrypsin (human protein), is being used to
treat emphysema. Sol.: It can survive temperatures upto 95°C. It is a
type of thermostable DNA polymerase.
171. Answer (1)
178. Answer (2)
Hint: The first successful clinical gene therapy in
Hint: Triphosphate nucleotides are a source of
humans.
energy.
Sol.: SCID can be treated by early bone marrow Sol.: ATP is not needed as deoxyribonucleotide
transplantation. W ithout T cells, ADA deficient triphosphates (dNTPs) can themselves provide
children are wide open to attacks of viruses and energy.
bacteria. In the absence of adenosine deaminase, 179. Answer (3)
lymphocyte proliferation is inhibited.
Hint: Escherichia coli Ry13.
172. Answer (3)
Sol.: The convention for naming these enzyme is
Hint: Tertiary structure of humulin. that the first letter comes from name of the genus
Sol.: Removal of C chain from proinsulin is required and second two letters come from the species of
to form mature insulin. In 1983, Eli Lilly an American the prokaryotic cell from which they were isolated.
company prepared two DNA sequences EcoRI comes from Escherichia coli RY13. In EcoRI,
corresponding to A and B, chains of human insulin, the letter ‘R’ is derived from the name of strain
introduced them in plasmid pBR322 and then ‘Rough’. Roman number following the name indicates
transformed E.coli to produce humulin. Disulfide the order in which the enzymes were isolated from
bonds are formed between cysteine residues on that strain of bacteria.
chain A and B in correct orientation. 180. Answer (2)
173. Answer (4) SPACE FOR ROUGH Hint:
WORKInsertional inactivation.
Hint: The Indian Parliament cleared the II nd
Sol.: Pst I lies in coding sequence of ampicillin
amendment of the Indian Patent Bills. resistance gene.
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