Course: BOT --- 525

Title: Botany - Service Course Basic Plant Tissue Culture

Sample Question set- 1

Id 1
Question Who is called as the Father of Plant Tissue Culture?
A Gottlieb Haberlandt
B Watson.
C Barbara.
D Crick.
Answer A
Marks 2
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Id 2
Question The instrument is necessary for drying the washed glassware are
A Vacuum pu
B Hot air oven
C eater.
D Autoclave
Answer B
Marks 2
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Id 2
Question Culture and maintenance of an excised organ primordial or whole or part of an _______
culture.
A Anther
B Callus
C Organ
D Suspension
Answer C
Marks 2
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Id 3
Question The culture of excised radicle tips of aseptically germinated seeds is termed as _______
culture.

A Anther
B Root
C Pollen
D Embryo
Answer D
Marks 2
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Id 4
Question High cytokinin and low auxin induces
A Root
B Shoot
C Callus
D Plant
Answer B
Marks 2
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Id 5
Question The commonly used cytokinin in meristem culture is
A Zeatin
B BAP
C IAA
D IBA
Answer B
Marks 2
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Id 6
Question A coconut milk in combination with gibberillic acid is highly suitable for the growth of

A Shoot
B Root
C Ste,
D Leaf
Answer A
Marks 2
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Id 7
Question A vegetative propagation of whole plants using tissue culture techniques is called as
A micropropagation.

B somatic embryogenesis.

C callus culture.

D protoplast culture.
Answer A
Marks 2
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Id 8
Question An undifferentiated and highly proliferative mass of cells is defined as
A Callus
B Explants
C Shoot
D Root
Answer A
Marks 2
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Id 9
Question Which plant growth hormones is highly required for growth of shoot
A Auxin
B Cytokinin
C Auxin
D Ethylene
Answer B
Marks 2
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Id 10
Question Which plant growth hormones is highly required for cell division
A Auxin
B Cytokinin
C Auxin
D Ethylene
Answer B
Marks 2
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Id 11
Question Callus is yellow in colour due to synthesis of _______ pigments
A Carotene
B Anthocynin
C Chlorophyll
D Anthocynin
Answer Phycocyanin
Marks 2
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Id 12
Question A single cell or small aggregates of cells can be easily multiplied by
A Micropropagation.

B Organogenesis.

C Callus culture.

D Suspension culture.

Answer D
Marks 2
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Id 13
Question The preservation of germplasm in the frozen state is defined a
A Cryoprotectant.

B Cryopreservation.

C Biopreservation.
D Storage
Answer B
Marks 2
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Id 14
Question Who reported the suspension cultures from carrot root explant
A Watson and Crick
B Steward and Shantz
C Benthaman and Hook
D Lederber and Tatum
Answer B
Marks 2
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Id 15
Question Which organism is known as natural genetic engineer of plants?
A Agrobacterium tumefaciens
B Escherichia coli.
C Bacillus sp.
D Streptomyces sp.
Answer A
Marks
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Id 16
Question is the in vitro aseptic culture of cells, tissues, organs or whole plant under controlled
nutritional and environmental conditions often to produce the clones of plants
A Tissue culture.

B Organ culture.

C Morphogenesis.
D Organ
Answer A
Marks 2
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Id 17
Question The production of adventitious roots and shoots from cells of tissue culture is termed as

A Organogenesis.
B Micropropagation
C callus culture
D suspension culture
Answer A
Marks 2
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Id 18
Question The developmental pathway of numerous well organized small embryoids is defined as

A Organogenesis.
B Mmicropropagation
C somatic embryogenesis
D suspension culture
Answer C
Marks 2
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Id 19
Question The zygote embryo enclosed by protective coat developed from integument is called
A Artificial seeds.

B Embryo.

C Anther.
D Pollen
Answer A
Marks 2
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Id 20
Question Which culture technique is used for the development of haploid plants
A Anther
B Callus
C Embryo
D Explant
Answer A
Marks 2
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Id 21
Question The in vitro development of haploid plants is called as
A organogenesis.

B Androgenesis.

C Embryogenesis.
D Micropropagation
Answer B
Marks 2
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Id 22
Question Microspore behaves like a zygote and undergoes change to form embryoid is known as
A Direct androgenesis
B indirect androgenesis
C Anther cult
D Pollen culture
Answer A
Marks 2
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Id 23
Question Two or more adjoining somatic protoplasts are fused without any inducer is called as
_______ fusion
A spontaneous.

B induced.

C Protoplast.
D Ssomatic cell
Answer A
Marks 2
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Id 24
Question The genetic variability regenerated during tissue culture is termed as

A somoclonal variation
B organogenesis
C micropropagatio
D androgenesis.
Answer A
Marks 2
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Id 25
Question Part of the plant taken for tissue taken for tissue culture
A Plant
B Explant
C Tissue
D Cell
Answer B
Marks 2
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Id 26
Question MS Media stands for
A Morris and Sing
B Morgan and shigella
C Murashige and Skoog
D None of the above
Answer C
Marks 2
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Id 27
Question Who is called as the Father of Plant Tissue Culture?

A Gottlieb Haberlandt.

B Watson.

C Barbara.

D Crick.
Answer A
Marks 2
Unit Ignore_this_row

Id 28
Question The instrument is necessary for drying the washed glassware are

A Vacuum pump. .

B Hot air oven

C Heater.

D Autoclave.

Answer B
Marks
Unit Ignore_this_row

Id 29
Question The zygote embryo enclosed by protective coat developed from integument is called

A artificial seeds.

B embryo.

C anther.

D pollen.

Answer A
Marks 2
Unit Ignore_this_row

Id 30
Question The broad spectrum herbicide is _____

A glyphosate. .

B 2,4-D

C phosphinothricin.

D imidazolinones.

Answer B
Marks 2
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Id 31
Question The carbohydrates used mainly in plant tissue culture is _____.

A sucrose.

B mannose.

C glucose.

D lactose.

Answer A
Marks 2
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Id 32
Question The optimal pH for plant tissue cultures is in the range of _____.

A 2-3.

B 3-4.

C 4-5.

D 5-6.

Answer D

Marks 2
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Id 33
Question The process for the fusion of a normal protoplast with an enucleated protoplast is _____.

A hybridizaion.

B cybridizaion.

C rDNA technology.

D transgenesis
Answer B
Marks 2
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Id 34
Question A cytoplasmic hybrid refers to _____.

A cybrids.

B chloroplast.

C tonoplast.

D protoplast.
Answer A
Marks 2
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Id 35
Question Which culture technique is used for the development of haploid plants?

A Anther.

B Organ.

C Callus.

D Embryo.
Answer A
Marks 2
Unit Ignore_this_row

Id 36
Question Plant growth regulators influence growth, metabolism and ________ of cultured cells.

A differentiation.

B elongation.

C enlargement.

D duplication.

Answer A
Marks 2
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Id 37
Question What type of glasswares can be used in the plant tissue culture work?

A Borosilicate.

B Plastic.

C Metal.

D Aluminum.

Answer A
Marks 2
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Id 38
Question The optimum relative humidity of plant tissue culture is _______%.

A 20-98.

B 30-98.

C 40-98.

D 50-98.
Answer D
Marks 2
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Id 39
Question The dry heat methods include _______.

A flaming. .

B incineration

C hot air oven.

D all the above.
Answer B
Marks 2
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Id 40
Question The optimum condition of autoclaving in plant tissue culture is ___ Â, ___ psi, ____min

A 123ºC, 15 psi, 15 min.

B 121 ºC, 17 psi, 15 min.

C C. 121 ºC, 15 psi, 25 min.

D 121 ºC, 15 psi, 15 min.

Answer D
Marks 2
Unit Ignore_this_row