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Alwadi International School Biology Grade-9 6.plant Nutrition Notes
Alwadi International School Biology Grade-9 6.plant Nutrition Notes
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ALWADI INTERNATIONAL SCHOOL BIOLOGY GRADE-9 6.PLANT NUTRITION NOTES
6.1 PHOTOSYNTHESIS
All living organisms including plants need food. They need it as a source of energy and to
build new cells and tissues as they grow. However, plants have the ability to produce
their own food from the raw materials around them by photosynthesis. Hence, plants
are called Autotrophs.
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ALWADI INTERNATIONAL SCHOOL BIOLOGY GRADE-9 6.PLANT NUTRITION NOTES
ADAPTATION FUNCTION
• They have a large surface area to absorb more light.
Large surface
• There will be a larger number of stomata, so more gas
area
exchange, therefore more photosynthesis.
• These are small holes in the lower side of the leaf which
allow diffusion of oxygen and carbon dioxide.
Many stomata
• It allows water vapour to diffuse out. Guard cells control
the size of stomata.
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ALWADI INTERNATIONAL SCHOOL BIOLOGY GRADE-9 6.PLANT NUTRITION NOTES
The figure below shows a drawing of the internal structure of a dicot leaf.
The figure below is a photomicrograph of the internal structure of a leaf. Label the parts.
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ALWADI INTERNATIONAL SCHOOL BIOLOGY GRADE-9 6.PLANT NUTRITION NOTES
The table below describes various parts in a dicot leaf and their adaptations.
ADAPTATION FUNCTION
Lower
epidermis • This acts as a protective layer. Stomata are present in it.
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CHOMATOGRAPHY
The leaves of plants contain chlorophyll as well as other
pigments that are used in photosynthesis to trap light.
Some students investigated the pigments present in a
green leaf using chromatography.
The process of chromatography separates the pigments.
The more soluble the pigment the further it moves.
The steps were as follows:
1. By pressing the leaf using a metal disc the pigments from a green leaf were transferred
to a strip of chromatography. A green line was then visible on the paper as shown.
2. The strip of chromatography paper was lowered into the test-tube and was held using
a stopper at the top as shown in the figure.
Result: After 15 minutes the paper was removed from the test-tube and the result is as
shown. Three pigments can be seen separated onto the paper.
In another investigation a leaf was grinded with ethanol and the extract was obtained.
This extract was used in chromatography. This resulted in the separation of 4 pigments
from the leaf extract as shown in the figure below.
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ROLE OF STOMATA
❖ The stomata in a leaf affect the rate of photosynthesis when they open or close.
❖ When photosynthesis is taking place, carbon dioxide in the leaf is being used up and
it’s concentration fall so the stomata will open. Thus, when the rate of
photosynthesis is very high, the stomata will open, allowing carbon dioxide to
diffuse into leaf.
❖ When the light intensity falls, photosynthesis will slow down and the buildup of carbon
dioxide from respiration will make the stomata close.
❖ In this way, the stomata are regulated by the rate of photosynthesis.
❖ Therefore, the stomata are usually open during daylight and are closed at night.
❖ Changes in the turgor and shape of the guard cells can open or close the stomata.
• When potassium ions concentration increases in the guard cells, it lowers the water
potential. Hence, more water move in by osmosis raising the turgor pressure and the
cell expands.
• The cell wall next to the stomatal pore is thicker than the rest of the cell and is less
able to stretch.
• Although the whole guard cell expands, the thick inner wall cannot expand much.
This causes the guard cells to curve so that the stomatal pore between them is
opened.
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❖ Plants take in carbon dioxide for photosynthesis and produce oxygen. They take in
oxygen for respiration and produce carbon dioxide.
❖ During daylight hours, plants are photosynthesizing as well as respiring, so all the
carbon dioxide produced by respiration is used up in photosynthesis.
❖ This means the oxygen needed by respiration is provided by photosynthesis.
❖ However, when the rate of photosynthesis is faster than the rate of respiration, carbon
dioxide will be taken in and the excess oxygen given out.
The table below describes gas exchange in plants.
Conditions Day time/ Presence of bright Night/dark
light
Processes Photosynthesis and respiration Only respiration but no
both occur.
occurring photosynthesis
But Photosynthesis rate is
greater than respiration rate
Figure to
demonstrate
COMPENSATION POINT
❖ Plants only carry out photosynthesis in light. But respiration happens 24 hours a day.
❖ During the day they take in more carbon dioxide for photosynthesis than that
produced in respiration. It is important that carbon dioxide uptake during the day
is greater so that more food is made than that used at night. This allows surplus food
to be produced, so surplus energy is available and growth is possible.
❖ In the dark,the rate of respiration greatly exceeds, so they release more carbon dioxide.
❖ As the light intensity increases after dawn, there comes a point where the rates of
respiration and photosynthesis exactly balance one another and there is no net
uptake or loss of carbon dioxide or oxygen.
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ALWADI INTERNATIONAL SCHOOL BIOLOGY GRADE-9 6.PLANT NUTRITION NOTES
❖ This is called the compensation point, and at this point the glucose consumed by
respiration is exactly balanced by the glucose produced during photosynthesis.
❖ The compensation point is the light intensity at which the rate of carbon dioxide
production during respiration is equal to the rate of carbon dioxide consumption
in photosynthesis. The sugar produced by photosynthesis exactly compensates for
the sugar broken down by respiration.
EXPERIMENT:
To demonstrate gas exchange in aquatic plant using hydrogen carbonate indicator.
Hydrogen carbonate indicator is used to indicate CO2 concentration in a solution. The
table below shows the colour change in the indicator at different concentrations of CO2.
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Observation:
IN THE DARK
(covered with black paper) IN LIGHT
START POINT
paper removed for (near a lamp)
photograph
• Normal CO2 • Respiration occurs but • Both processes occur, but more
• Indicator colour not photosynthesis photosynthesis than respiration
remains red. • Indicator turns yellow • Indicator turns purple as less
because more CO2 is CO2 is present as it is being
produced in respiration removed for use in
photosynthesis.
Results and conclusion: The experiment proves that gas exchange occur in plants, and
that in photosynthesis they use carbon dioxide and release oxygen, and in respiration they
use oxygen and release carbon dioxide. Respiration occurs day and night in plants but
photosynthesis occurs only during daytime or when there is a source of light.
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1. Used in respiration: The sugar can be used to provide energy. It is broken down by
respiration to carbon dioxide and water, and the energy released is used in other
chemical reactions. Ex: To absorb ions by active transport.
2. Stored as starch:
• Glucose is a very soluble and reactive substance. It may dissolve in the cytoplasm
and increase the concentration within the mesophyll cells of the leaf. This could
disturb the osmotic balance between the cells.
• Starch is a relatively insoluble compound and does not alter the osmotic potential of
the cell; therefore glucose is stored as starch.
• Starch is stored in special organelles, called amyloplasts found in stems or roots or
in storage organs called tubers such as potatoes.
• The starch, however, is steadily broken down to sucrose and this soluble sugar is
transported out of the cell to all parts of the plant that do not photosynthesize, like the
growing buds, the ripening fruits, the roots and the underground storage organs.
3. Formation of cell walls: By joining hundreds of glucose molecules, long chains of
cellulose are formed which are used to build cell walls. Lignin found in the xylem is
also produced using glucose.
4. Formation of cell membranes: Plants can also convert glucose into fats/oils for
storage in seeds or to be used in the formation of cell membranes.
5. Formation of proteins or enzymes: Amino acids are made by combining nitrogen
and sulfur which are absorbed as nitrates and sulfates respectively from the soil.
Amino acids are joined to make proteins which form enzymes & cytoplasm in the cell.
6. Formation of other molecules: DNA is formed by addition of phosphorus which is
taken up as phosphates (PO4). DNA consists of a pentose sugar. Chlorophyll molecule
is formed by addition of magnesium (Mg). This is also obtained from salts in the soil.
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2. Heat the leaf in hot To remove chlorophyll from leaf, as it will mask the
ethanol clear observation of any colour change. Ethanol
dissolves cell membranes and chloroplast
membranes.
3. Wash with hot/cold water To rinse the leaf and soften it.
4. Spread onto a white tile To observe the presence of starch which is indicated
and add iodine solution. by the appearance of the blue/black colour.
Safety precautions:
Note:
It is not possible to remove chlorophyll from a leaf without killing it, so a variegated
leaf, is used in the experiment.
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✓ Destarching the plant ensures that all the stored starch is used up to make sure
that the starch to be tested is formed during the experiment.
✓ Potted plants are destarched by leaving them in a dark cupboard for a few days.
In the darkness, any starch in the leaves will be changed to sugar and carried
away from the leaves to other parts of the plant.
✓ For plants in the open, the experiment is set up the day before the test. During the
night, most of the starch will be removed from the leaves.
Control: The part of the leaf exposed to sunlight will be the control for the experiment.
Any uncovered leaf from the same plant can also be taken as control.
Results and Conclusion: Only the uncovered parts of the leaf become blue-black with
iodine solution, showing the importance of light in photosynthesis
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Results and Conclusion: As the light intensity increases (lamp moved nearer) the
number of oxygen bubbles formed per minute also increase so the space created by
the oxygen at the top also increases, but after a limit the number of oxygen bubbles
made per minute remains constant.
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2. Keep light intensity the same by keeping the lamp at a fixed position.
3. Concentration of CO2 should be constant by adding a fixed amount of sodium
hydrogen carbonate.
4. Maintain a temperature of 25o C. Count the number of bubbles after 5 minutes.
5. Increase the temperature by 5 degrees every ten minutes. This can be done with the
help of a hot plate which is placed below the setup and is thermostatically controlled.
6. Count the number of bubbles released at each temperature.
Results and Conclusion: As the temperature increases the number of oxygen bubbles
formed per minute also increase, but after a limit, the number of oxygen bubbles
started to decrease and finally the release of oxygen stops. We can conclude that rate of
photosynthesis increases with increase in temperature until the optimum temperature.
After that the rate of photosynthesis decreases and will finally stop because the enzymes
will get denatured.
Error: The exact number of bubbles of oxygen produced cannot be counted. Moreover,
every bubble will not be of the same size, so the results will not be reliable.
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1. Take two healthy potted plants and destarch them by placing them in the dark for a
few days.
2. Place a container of sodium hydrogen carbonate on the soil of one plant. It adds
carbon dioxide to the environment.
3. Place a container of sodium hydroxide on the soil of the second plant. It removes
carbon dioxide from the environment. This will serve as a control.
4. Cover both plants with a glass jar and leave them in the sun for several hours.
5. Pluck a leaf from each plant and perform the starch test on each leaf.
Precaution: The class jar must be kept airtight especially in setup B, to prevent any
carbon dioxide in the air from entering the jar.
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❖ Farmers can use their knowledge of factors limiting the rate of photosynthesis to
increase crop yields. This can be achieved in greenhouses, where the conditions are
more easily controlled than in the environment outside.
❖ In greenhouses, however, the additional cost of providing extra lighting, heat and
carbon dioxide should be cost effective and must not exceed the additional income it
generates for the farmer.
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ALWADI INTERNATIONAL SCHOOL BIOLOGY GRADE-9 6.PLANT NUTRITION NOTES
5. Humidity: Ventilation (by the use of windows) allows some control of the humidity and
also the temperature.
6. Computer controlled sensors can be used to provide information about factors such
as water, humidity, temperature, carbon dioxide concentration etc. so any changes are
easily corrected.
7. Ventilators: These can be opened to allow carbon dioxide to enter during the day &
allow oxygen to enter at night. When opened they allow water vapour to escape and
thus prevent the air from becoming too humid thereby reducing chances of fungal
diseases. Ventilators allow fresh air to move in and decrease temperature on hot days,
so plants are not overheated.
By using a combination of these techniques, the horticulturalist can produce early crops
and maximize photosynthetic yield.
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ALWADI INTERNATIONAL SCHOOL BIOLOGY GRADE-9 6.PLANT NUTRITION NOTES
❖ Plants need to take in a number of elements to stay alive. The most important ones
include: carbon, hydrogen &oxygen.
❖ In addition to these three elements, plants need a number of minerals for healthy
growth. They are absorbed through the roots by active transport as the mineral ions
are dissolved in the soil water.
❖ If any mineral element is deficient in the soil, then the plants may show deficiency
symptoms.
Two important mineral ions needed by plants and their uses are given in the table:
MINERAL USE/IMPORTANCE DEFICIENCY SYMPTOMS
1. NITRATE
2. MAGNESIUM
❖ The mineral elements needed by plants are absorbed from the soil in the form of
salts which usually come originally from rocks, broken down to form the soil.
❖ They are continually being taken up from the soil by plants or washed out of the soil
by rain. They are replaced partly from the dead remains of plants and animals. When
these organisms die and their bodies decay, the salts are released back into soil.
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❖ In arable farming, the ground is ploughed and whatever is grown is removed. There are
no dead plants left to decay and replace the mineral salts. The farmer must replace
them by spreading animal manure, sewage sludge or artificial fertilisers in measured
quantities over the land.
❖ Three manufactured fertilisers in common use are ammonium nitrate, superphosphate
and compound NPK.
DEMONSTRATION OF THE IMPORTANCE OF DIFFERENT MINERAL ELEMENTS
❖ It is possible to demonstrate the importance of the various
mineral elements by growing plants in water cultures.
❖ A water culture is a solution containing the mineral salts
which provide all the necessary elements for healthy growth
as shown in the figure on the right.
❖ In an investigation four wheat seedlings-W, X, Y, Z were
placed in test-tubes containing water cultures. Each setup
had different conditions as shown in the figure below.
Results:
W Seedling with nitrates and magnesium show normal growth. This is the control.
X In the absence of nitrogen plants shows little growth with small, pale leaves.
Y In the absence of magnesium plants show yellow leaves with poor growth.
Z In absence of both minerals there is hardly any growth in the seedlings
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Nov-17 11/22
June-18 11/21,22 3/43 2b/62
June 19 10,11/21,22,23 2/41,42,43
Nov 19 10,11/21,22,23 --
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