LYCEUM OF THE PHILIPPINES UNIVERSITY CAVITE

Effectiveness of Avocado (Persea Americana) and Calabrese broccoli

(Brassica oleracea var. italica) Extract as Hair Growth Spray

An Investigatory Project
Submitted to the Faculty of the International School
Lyceum of the Philippines University Cavite

In Partial Fulfillment of the Requirements of the

International School, Senior High School Program
(Science, Technology, Engineering, and Mathematics)

BAUTISTA, CHRIS JOHN E.

BELTRAN, CHRISTIAN A.

ESTOYA, KRISTINE R.

GOMEZ, NAZEL V.

May 2020

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Chapter I

INTRODUCTION

Background of the Study

Everybody's hair is unique, and loads of variables can impact how rapidly it

develops. Various factors, including inherited characteristics, gender, and age can affect

the rate of hair improvement. Taking care of hair is an indication of Healthy hair, some

doesn’t that result from having unhealthy hair, scalp and moderate hair growth. Hairs are

somewhere ranged per month as of 0.5 and 1.7 centimeters. This is proportional to around

0.2 to 0.7 inches. Hair items (shampoos or nutrient enhancements) have not been

appearing to perceptibly change this rate. This gauge originates from The Trichological

Society (Barrell, 2019).

As times go by many individuals experiencing baldness, it is a hair loss or the

absence of hair, the clinical term for balding is alopecia. There are also many reasons and

symptoms that depend on the type, such as male and female baldness as well as aging,

family history of baldness, dandruff, poor circulation of the scalp and etc. (Cole, 2019).

Hair may basically thin on account of fated inherited components and the general

developing procedure. Numerous people may see a mellow physiologic diminishing of

hair beginning in their 30s and 40s. As people get progressively prepared, their hair may

start to lose volume and thickness Now and again, diminishing hair is connected with

diet, supplements insufficiencies, or inherited male and female pattern baldness. As a

rule, diminishing hair isn't connected with general wellbeing concerns. Be that as it may,

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it can influence individuals' psychological wellness and confidence. Individuals can

attempt a few medications and home solutions for support hair wellbeing, thickness, and

quality (Stinson, 2019).

According to Tadimalla (2019) broccoli is a green plant that is considered as a

superfood. It is one of the most advantageous cruciferous vegetables, whose huge

blossoming head is regularly eaten for its dietary properties. It is regularly expended

bubbled or steamed, yet can likewise be eaten crude. On the other hand, Broccoli is a

great source of Vitamins and helps in hair growth. Indeed, one investigation demonstrated

that ingesting cruciferous veggies like broccoli can shield cells from DNA harm. Stacked

with potassium, and amino acids, this rich influence nourishment are difficult to beat.

Avocados are normal item with a smooth surface that has a different benefit. There are

also potential health benefits that avocados can do, aside from that Avocados has a

vitamin C, E, K, and B-6. (Ware, 2017).

This study wants to know the effectiveness of Avocado (Persea americana) and

Calabrese broccoli (Brassica oleracea var. italica) extract as a hair growth spray. The

researchers want to give new innovations in the field of Medical Science. This study only

focuses on the effectiveness of the organic product for hair growth.

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Statement of the Problem

The goal of this study is to give knowledge and to know the effectiveness of Avocado

(Persea americana) and Broccoli (Brassica oleracea var. italic) as a hair growth spray.

1. What are the effectiveness of avocado fruit and broccoli leave and stem in terms of:

A. Length using Tape Measure

B. Width using Laser Pointer

2. What are the percentage of avocado fruit and broccoli stem and leaves used in the

experimental phase:

A. 70% extract of avocado fruit and 30% extract of broccoli stem and leaves

B. 50% extract of avocado fruit and 50% extract of broccoli stem and leaves

C. 30% extract of avocado fruit and 70% extract of broccoli stem and leaves

3. What are the differences between our product and other commercial product?

Hypothesis

In this study shows negative results in every process that the researchers do to make an

alternative product for hair growth.

1. There is no effect of broccoli and avocado extract during the experimentation.

a. For the length, there was no growth of hair happen after the experimentation.

b. The thickness of the hair doesn't have improvement after spraying the finished product.

2. The percentage here does not been effective in terms of stems and leaves.

a. The result of this kind of percentage is not good because of not equal percentage of a
certain extract.

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b. In this area, the percentage of each is not effective.

c. Same to the result of a because of its not equal percentage of the extract.

3. For the difference of our product to the commercialized product is about the process on

how we made it, but in the result, our product was not totally effective in achieving hair

growth also our product needs a lot of days and no. of spray in a day just to have a long

and easy growing of hair

Significance of the Study

In this study, the researchers include different beneficiaries that can benefit to the
alternative product for hair growth, these are the:

Future College Students. This study is beneficial for the upcoming college students who

create research that is similar to the process in this study. Also, in addition, the

advantages of this study to the colleges, especially those who take courses under the

STEM strand is to give an idea of what is the importance of the ingredient that they

should be used to create a new and effective product.

Teachers. To the teachers, this study can give some information about the chosen

ingredients and also to show the importance of each ingredient in our everyday life. The

teachers can also rely on this study and show to the students how important and role of

every ingredient in our body parts especially when it comes to our hair.

Community. This study is a benefit to the community because it can help a lot of people

who encountered and experiencing hair loss or hair fall. Most of the time, students and

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seniors experienced a kind of situation like this and of course, we the researchers want to

help those people and give them the easiest way to bring their hair back all over again.

Future Researchers. This study can give a lot of ideas and knowledge to the researchers

and also this can be a guide to them in creating a similar study about hair growth. It can

help them to process properly and make a new product in the future. The researchers can

also use this as a reference in their study.

Scope and Limitations

This study will cover the effectiveness of Avocado (Persea americana) and Broccoli

(Brassica oleracea var. italic) Extract as a hair growth spray. The materials needed and

the extract will be conducted only in Golden city subdivision Imus City Cavite. The

researchers will need 300 ml of extract to be partially divided into 100 ml per containers,

also will use a Laboratory rat that will be getting in Veterenary. The researchers will be

using a laser pointer to measure the width of the hair together with tape measure for the

length both will be tested and it will be conducted in Biology, Laboratory of Lyceum of

the Philippines University-Cavite. It will be conducted from March 2020 – April 2020.

Experimentation will be done in order to find out the effectiveness of Avocado and

Broccoli extract as a hair growth spray. The researchers will buy the Avocado and

Broccoli in SM Market Mall (Kadiwa Market) in Dasmariñas Cavite.

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Definition of Terms

These are the terms that are hereby defined operationally:

Hair is any of the fine threadlike strands developing from the skin of people, warm
blooded creatures, and some different creatures.

Hair Growth it is the process and the development of the hair and the protein called

keratin that makes up hair and animates hair development.

Diminishing it is the process of becoming less of hair when an individual reach the
certain age.

Variables are the number of data or elements that given in a certain problem or questions.

Scalp is a part of the head that helps to cover including the face and it is where the hair
grows.

Balding is the demonstration of gradually losing hair from the head. The procedure of

losing hair because of stress or intensity.

Calabrese broccoli is a quickly developing and exceptionally simple to develop crops

that are otherwise called Americana, Italian or green growing broccoli.

Alopecia is a loss of hair, wool and feathers and Another term for balding.

Tape Measure is an instrument that will be used to measure the length of the hair.

Laser Pointer is an instrument to be used to measure the width of the hair.

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CHAPTER II

LITERATURE REVIEW

Hair Growth

As stated by Semalty et al. (2010), hair has mental and sociological significance all

through the ages in confining the character and the outward presentation of a person.

Noteworthy advancement is being made on finding a viable and safe medication for hair

development.

Hair development is influenced by the topical use of a boron containing

mixes/buildings in which a focal tetrahedral molecule is covalently bound to four ligands.

The hair-development cycle includes three particular stages, the anagen, catagen, and

telogen periods of autonomous hair follicles. Hair piece keeps on developing throughout

the stage of anagen, trailed by the alteration of time of the catagen stage, which goes into

the telogen stage, when hair is discharged from the thread and dropped. The anagen stage

can be arranged into a proliferating anagen stage that includes the actuation of new hair

follicles, and an independent anagen stage, when hair development and separation of hair

piece threads effectively happen (Choi, 2018) .

Laboratory rats to very known as a human gene, eventually laboratory rats were

known to show age-related health issues, and the rat has a similar way to those humans.

For decades, laboratory rats and mice have been used to make great scientific and medical

advances. These are also easily trainable and perfectly suited for psychological

experiments and others in terms of skin, hair (Kershner, 2020).

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Hair growth serves a big role in our life. It serves as the foundation of the

development of our hair. Hair growth also depends on the product that you will apply, the

environment that you have and the physical characteristics also. Laboratory rats have a

multiple similar to humans.

Avocado

Avocado is a kind of fruit that is because of its shape it is known as an alligator pear,

fruit; it allows to supply healthy monounsaturated fatty acids (MUFA). Avocados consist

of 20 minerals and vitamins that will help our body to prevent heart disease, diabetes and

obesity also in addition, avocado can be used as a treatment for cancer (Ware, 2017). As

stated by Schwartz (2018) Avocado fruit has many purposes in different ways, making it

in a different style of food like smoothies, toast or stuffed avocado. It can also make as an

alternative treatment like a mask which is commonly used by other people nowadays.

As claimed by Ware (2017) various examinations have discovered that a dominatingly

plant-based eating regimen that incorporates nourishments, for example, avocados can

assist with diminishing the danger of stoutness, diabetes, coronary sickness, and when all

is said in done mortality while propelling a sound appearance and hair, extended

essentialness, and all things considered lower weight. Avocado's advantages are

increased your vitamins and minerals in your diet, helps your blood level and sugar level

to become normal, it helps your immune function, it lowers the risk of your heart disease,

your cholesterol and constipation; it can also an antioxidant in your body, aside from that,

this fruit contains a high percentage of fats which it can cause some conditions to your

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body like allergies, some adverse effects like migraine, flu and etc., can damage your

liver, can cause gastrointestinal irritation and can reduce the level of your cholesterol.

Avocado must be avoid to those people who are pregnant and doing breastfeeding and

hypersensitivity (Bruso, 2016).

Avocados are frequently utilized in healthy skin and other excellent medicines. There

are a few wonder items out there with avocado or avocado oil as a fixing—cleanser,

moisturizer, hand cleanser, lip demulcent, face cream, and so forth (Schwartz, 2018).

Avocado fruit is essentially fruit for hair growth. It utilizes also our skin and cleanses

our body. Avocado has many purposes that is why it is common use in giving vitamins

and medications. If our body lacks of nutrients avocado can really help us especially in

hair problems.

Broccoli

Broccoli and broccoli grow to contain a lot of glucosinolates. Various examinations

have validated the chemoprevention impact of expanding cruciferous vegetable admission

against malignant growth, which has been ascribed to the movement of different

isothiocyanates that are enzymatically hydrolyzed from glucosinolates (Li. et al., 2010).

According to Mukherjee et al. (2009) Broccoli vegetable consists of different kinds of

vitamins such as vitamin A, C, E, and vitamin K, Fiber, Iron, Calcium, Potassium,

Protein, Magnesium, Folic Acid and Selenium. The stem of broccoli because it contains

moderately calcium, iron, and vitamin C (Gil et al. 2014). According to Nagdeve (2020)

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It helps skin care and gives glowing appearances, health and appearance radiantly. The

nearness of vitamin C, sulfur, and amino acids make broccoli a great detoxifier.

According to Vasanthi et al. (2009) the cruciferous vegetables, which incorporate

broccoli, cabbage, and cauliflower are a superb wellspring of phytochemicals including

glucosinolates and their results, phenolics and cancer prevention agent nutrients just as

dietary minerals. Broccoli utilization intervenes an assortment of capacities, including

giving cancer prevention agents, managing compounds, and controlling apoptosis and

cell cycle. In addition, broccoli also has a great impact to our skin; it improves the health

of our skin and it also prevent and make our aging slowly. Broccoli has vitamin C and B

in which it gives a lot of roles in our body like making our skin look new and younger

while in having vitamin B, it can treat hair loss and improves our hair much better.

Broccoli has some good benefits to us like boosts immunity, improves our brain health,

liver health, bones and teeth, avoid heart disease and improves our metabolism. This

vegetable can also prevent our skin and body to some conditions like allergies and

diabetes (Shanks, 2019).

Broccoli contains a lot of vitamins in which those vitamins play a lot of roles in our

body and skin. This vegetable also used as a treatment in different ways in different

conditions that our body will experience. Also, in addition, most importantly, broccoli has

an impact to our hair in which it can also affect our physical characteristics. Broccoli

helps us to have a better version of our body, body system, skin, and hair.

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CHAPTER III

METHODOLOGY

Research Design

Pre-experimental design will be used in this study because there is no control or an

exact outcome because of the extraneous variable. The researchers want to see the

interventions will have some effect of the given variables. The pre- experimental

research design is to authorize researchers to approximate the outcome of an

experimental treatment (Ary et al., 2006). Thus, this study will provide a new way or a

new product to help for hair growing.

Locale of the Study

The experimentation will be conducted in the Biology Laboratory of Lyceum of the

Philippines University-Cavite (LPU-C) located in Manggahan, General Trias Cavite.

The extract will be conducted in Golden city subdivision Imus City, Cavite This study

will be conducted from March 2020 until April 2020.

Data Gathering Procedure

The researchers are the one who will prepare the materials needed in the experiment

and will purchase Avocado (Persea Americana) and Broccoli (Brassica oleracea var.

italica) to make an extract as the hair growth spray, the researchers will buy 9 mice and

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will be divided into 3 groups. The researchers will prepare 3 bottles of transparent spray

that is 100 ml containers for extract. The researchers will prepare a liquidizer for getting

the extract. The spraying process will be applied every morning and evening with the

distance of 15 cm and 5.9 in inches. The outcome of the product will be compared to the

commercial product which is the Aloe Hair Grower Shampoo. The result of the

experiment, documentation and observation will write it down that will give an insight to

the researchers. The results will be used to provide on what the research study needs.

Data gathering will be conducted in the year 2020.

Data Analysis

The gathered information will be studied in an Inferential statistic way. Inferential

statistics will be used to find accurate data in the research that has been conducted. This

statistical approach is suited to the researcher’s study. Two types of inferential statistics

will be used in this study to analyze the data: The T-test, and Two-way ANOVA test. The

result in the said tests will be used in the analysis of the study.

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