Ayesha Aslam

MULTIPLE CHOICE QUESTION

Translation

1. Translation is the
a) synthesis of DNA from a mRNA template
b) synthesis of protein from a mRNA template
c) synthesis of RNA from a mRNA template
d) synthesis of RNA from DNA template
2. Translation occur in the
a) nucleus
b) lysozyme
c) nucleolus
d) cytoplasm
3. _3 During translation protein are synthesized
a) by ribosome using the information on DNA
b) by ribosome using the information on mRNA
c) by lysosome using the information on DNA
d) by ribosome using the information on rRNA
4. The enzyme involved in amino acid activation
a) Aminoacyl tRNA synthetase
b) ATP synthetase
c) Aminoacyl mRNA synthetase
d) Aminoacyl rRNA synthetase
5 which of the following RNA molecules serves as an adapter molecule during protein synthesis
a) rRNA
b) mRNA
c) tRNA
d) tRNA and mRNA
6 In prokeryotes the first amino acid in polypeptide chain is
a) methionine
c) N- methyl methionine
d) N formly methionin
7 .During translation the role of enzyme peptidyl transferase is
a) transfer of phosphate group
b) amino acid activation
c) binding if ribosome subunits to mRNA
d) Peptide bond formation between adjacent amino acids
8.polysomes are
a) aggregation of ribosome
b) aggretion of lysosomes
c) mRNA molecules to which many ribosomes are attached simultaneous
d) All of these
9 Which is energy rich molecule required for initiation of translation
:
‘(a)ATP (b) GTP (c) CTP (d) AMP
10 -IN eukaryotes translation in initiated by binding of ribosome to the
(a) poly A tail (b) hogness box (c) pribnow box (d) 5`cap
11-hsp 60 and 70 are protein involved in
(a) initition of translation (b) elongation of translation (c) termination translation (d)
protein folding
12 .The anticodon of tRNA binds with
(a) codon of tRNA (b) codon of rRNA (c) nucleic bases of mRNA (d) Amino acid
13.Which step of translation does not consume a high energy phosphate bond
(a) translocation (b) amino acid activation (c)aminoacyl tRNA binding to A site
(d)peptidyl transferase reaction
14 Elongation of peptide chain involved in all except
(a)formyl met tRNA (b)mRNA (c)GTP and peptidyl (d) rRNA
15 The nonsense codon are
( a) UAG (b)UAA (c) UGA (d) ALL of these

16- Three step codon which aids in termination of translation are also known as

(a) non sense codon (b) antisense codon (c) missense codon (d) pause codon
17-Site in the ribosome from which the tRNA donates amino acid to the growing polypeptide
chain is ;
(a) P site
(b) E site
(c) O site
(d) T site

(18)What are the svedberg values for the subunit of the 70S ribosome of E.coli;
(a) 40S and 30S
(b) 50S and 30S
(c) 40S and 20S
(d) 50S and 20S
(19) Erf1 is the release factor in eukaryotes that requires
(a)ATP for its binding to ribosome
(b) GTP for its binding to ribosome
(c )ATP and GTP for its binding site
(d)Mn2+ for its binding site
(20) Which of the following bacterial operon is not controlled by attenation
(a) arabinose
(b) tryptophan
© leucine
(d)histidine
(21) The lac operon express the gene whose product catabolize
(a) maltose
(b) phosphate
© sucrose
(e) Lactose
(22) Splicing protects from RNA.
(a) replication
(b) amplification
( c) degeneration
( d) degradation
(23) An operon is made up of
(a) promoter
(b) operator
© structural gene
(d )all of the above
(24)Non coding RNAs regulate
(a) RNA splicing
(b) DNA replication
© gene regulation
(d)All of the above
(25) Adding a phosphate group to protein is called .
(a)Replication
(b)Hydrogenation
©Phosphorylation
(d)Carbation
(26) The small subunit of the ribosome binds to the 5 end of
(a)mRNA
(b)tRNA
©Rrna
(d)DNA
(27). This best describes a polysome

(a) active site for synthesis of lipids

(b) active site for synthesis of proteins

(c) active site for synthesis of DNA

(d) all of these

(28). In protein synthesis, translocation is initiated with the movement of

(a) tRNA from P-site to the A-site

(b) dipeptidyl tRNA from A-site to P-site

(c) tRNA from A-site to P-site

(d) tRNA from P-site to E-site

(29). The process by which protein synthesis from genetic code occurs is best described
by

(a) transcription

(b) translation

(c) replication

(d) reproduction

(30). This is incorrect about the nature of genetic code.

Codons are

(a) universal

(b) overlapping

(c) commaless

(d) triplet

(31) This elongation factor is known as translocase

(a) EFG

(b) EF2

(c) both (a) and (b)

(d) EF-Tu and EF-Ts

(32). This drug inhibits the initiation step of translation

(a) ricin

(b) tetracycline

(c) streptomycin

(d) cyclohexylamine

(33). In translation, this is not an essential component

(a) amino acid

(b) ligase

(c) mRNA

(d) anticodon
 (34)This identifies a particular amino acid and its cognate tRNA molecule

(a) topoisomerase

(b) rRNA

(c) Ribosome

(d) tRNA synthetase

(35). Protein synthesis corresponds to the process of

(a) duplicating required DNA for synthesis of proteins

(b) formation of amino acids from mRNA

(c) formation of mRNA from DNA template

(d) formation of amino acids from DNA template directly

(36). This is considered to be the start codon

(a) AGG

(b) UAG

(c) GUG

(e) AUG
(37)Tetracyclin blocks protein synthesis .
a) inhibiting binding of aminoacyl tRNA ribosome
b) inhibiting initiation of translation
c) inhibiting peptidyl transferase
(d) inhibiting translocase enzyme
(38) A sequence of codon that runs from a specicific start site to a terminating codon
called a
(a)frame
(b) reading frame
©reading site
(d) reading cell