I. Comparison in detail between Rapidchek Select Salmonella, 3M Petrifilm Salmonella Express, Neogen 2.

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Reveal Salmonella
No. Description Rapidchek Select Salmonella
1 Packing Insert Rapidchek SELECT Salmonella Food
System 7000190
1. Rapidchek SELECT Primary
Media 500 g
2. Rapidchek SELECT
Supplement 250 ml
3. Rapidchek SELECT Secondary
Media 10 g
4. Test Strip 2 x 50 test
5. Transfer pippettes and tubes
2x 50 test
Neogen 2.0 Reveal
3M Petrifilm SALX
Salmonella
Reveal test kit : 3M Petrifilm Salmonella Express
1. Reveal Salmonella 20 test devices, Consists of :
strips 1. 3M Salmonella enrichment
2. Dispossable transfer pipettes Base
3. Reaction cups 2. 3M Salmonella Enrichment
3. 3M Petrifilm Salmonelle
A lot of media required but not provided Express Plate
(depending on sample ) 4. 3M Salmonella Express
Confirmation Disk
Revive Media Kit : *All packaged separately
Revive medium, stomacher type bag,
graduated cup
Secondary selective Enrichment kit :
2x Rappaport Vassiliadis media, stomacher
bags, graduated cup
Direct selective enrich kit:
1x Rappaport Vassiliadis media, stomacher
bags, graduated cup
M-Broth post enrich kit :
M-Broth media

Lactose broth
Sample Sample preparation : There are so many enrichment media for Sample preparation :
Preparation sample preparation depending type of
High Microbial sample : Hi Microbial
1st enrichment 16-22 hrs @42 °C Revive medium, RV medium, M-Broth and 1st enrichment 18-24 hrs @41,5 C
2nd enrichment 16-22 hrs @42 °C Lactose broth medium 2nd enrichment 8-24 hrs @41,5 C
 Low Microbial Step : Low Microbial
1st enrichment 16-22 hrs @42°C 1. Pre-Enrichment 4 hours at 36 ± 1st enrichment 18-24 hrs @41,5 C
2nd enrichment 6-8 hrs @42°C 1°C Environment
2. Enrichment incubate 16-24 hours at
42°C, Plate Hydration:
3. some of type samples there is 1. Hydrate one plate w/ 2 ml
additional post incubation 6 hours sterile water / BPD
at 36 ± 1°C 2. Wait 1 min @20-25C

3 Reagent System ELISA Method – Lateral flow Strip
Enrichment Medium :
RC SELECT Supplement contain
Bacteriophage – patented
Romerlab. As Selective Agent, To
ensure specific growth for salmonella
Uses utilizes specific immobilized
antibody salmonella labelled with
colloidal gold
ELISA Method - Lateral flow strip,
There are many types of Enrichment step
procedure depending on type of matrices.
There are Revive media, Rappaport-
Vassiliadis Media, M-Broth, and Lactose
broth, all this are not provided in Reveal Kit.
Conventional method =
Chromogenic media
• Can not differentiate
lactose (+) salmonella with
other lactose (+) organisms.
• Lactose (+) salmonella will
appear as non-salmonella.
All the enrichment media (3M
Salmonella Enrichment Base, 3M
Salmonella Enrichment Suppl
Rappaport Vassiliadis R1) Use
common ingredients like any other
DCM.

4 Instrument Incubator 42 °C Need 2 Type of Incubator, capable of Incubator 41,5 C

maintaining 42° C and 36±1° C
5 Result Presumptive Positive : Presumptive Positive : Presumptive positive :
Interpretation 1 line Negative Negative result : 1 line • Red to brown colonies with
2 lines Positive Positive result : 2 line yellow zone or associated
with gas bubble, or both
• So many indicator to
interprete, need to use
Confirmation : interpretation table.
• It is very critical to use a
permanent ultrafine tip
 From secondary Enrichment,
continue to 2 Selective Agar plate
accoding to ISO 6579:2002
6 Time 22-30 hrs for Low micobial spls
32-44 hrs for high microbial spls
Detection time test strip :
10 minutes
SDIX is the manufacturer of antibody
test strip
marker to mark the
presumptive colonies.
Confirmation :
3M Pertrifilm SALX Confirmation
disk without subculture, microbial
recovery are questionable.
Time release : 20-28 hours. 18-24 hrs for low microbial spls
Sample with matrices may contain residual 26-48 hrs for high microbial spls
antigen, highly viscous, highly pigmented
material, dry pet Food, leafy greens, fresh
spinach there is additional post incubation 6
hours at 36±1°C
Difficult handling in preparation sample
enrichment, when using sterile-purified
water prewarmed to 36°C and 42°C

Testing time strip test :

15 minutes
7 Inclusivity 100% specifity High false negative
Serovars that are not within somatic groups High possibility of False Negative
A-E will not detected because :
• Can not differentiate
lactose (+) salmonella with
other lactose (+) organisms.
• Lactose (+) salmonella will
appear as non-salmonella.

8 Sensitivity 1 CFU/ 25 gr samples 1-5 CFU in 25 gr samples No data

9 Approval AOAC PTM (#080601) AOAC PTM (#960801) AOAC PTM (#061301)
II. Comparison in Detail between Rapidchek SELECT Salmonella, Chromogenic Salmonella, Rambach Agar
No. Description Rapidchek SELECT Salmonella
1 Packing Insert Rapidchek SELECT Salmonella Food
System 7000190
1. Rapidchek SELECT Primary Media
500 g
2. Rapidchek SELECT Supplement
250 ml
3. Rapidchek SELECT Secondary
Media 10 g
4. Test Strip 2 x 50 test
5. Transfer pippettes and tubes 2x
50 test
Sample Preparation Sample preparation :
High Microbial
1st enrichment 16-22 hrs @42 °C
2nd enrichment 16-22 hrs @42 °C
Low Microbial
1st enrichment 16-22 hrs @42C
2nd enrichment 6-8 hrs @42C
3 Reagent System ELISA Method – Lateral flow Strip
Enrichment Medium :
Chromogenic Salmonella
1. Chromogenic Salmonella Agar Base
(REF 4053502)
2. Salmonella selective supplement,
5+5 Vials supplement A & B (REF
4240013)
2 Type procedure :
1. Direct plating from Salmonella
isolation
2. Plating after Enrichment medium
step in selective or non-selective
media, 37°C incubation 18-24
Hours. Then Biochemical test
➢ Chromogenic medium and
selective compounds of medium
➢ The selectivity of medium is
improved by a cephalosporin which
Rambach Agar w/ Salmosyst suppl
1. Rambach Agar Kit for 4 x 250 ml
includes :
a. 4 Vials of Nutrient Powder
b. 4 Vials of liquid Mix for 4 x 250 ml
culture
2. Salmosyst Broth includes :
a. 500 g Salmocyst broth base
b. 250 Tablet Selective suppl
Enrichment :
Preliminary enrichment
Incubate 6-8 hrs at 35 °C
Transfer 10 ml culture to sterile test tube
Selective Enrichment :
Add 1 table salmosyst selective suppl and
wait 30 minutes
Shake vigourously, incubate further 18-22 hrs
at 35 °C
Innoculation :
Innocculate ¼ of the agar surface
*the inoculation
shall be continued with the same loop over
the rest of the plate.
Incubation: Aerobically at 35–37 °C for 24–48
hours.
Differential-diagnostic Culture medium
Utilize nutiritive substrates for
Enterobacteriaceae, + additional Sodium
Deoxychalate & Propylene Glycol to
 RC SELECT Supplement contain inhibits the growth of differentiate from gram posistive flora, and
Bacteriophage – patented Romerlab. As pseudomonas, suppress the growth then additional -galactosidase to
Selective Agent, To ensure specific growth of gram-positive and some gram- differentiate from coliform bacteria.
for salmonella negative bacteria by sodium
deoxycholate, and Tergitol inside is
Uses utilizes specific immobilized active mainly against the growth of
antibody salmonella labelled with Proteus spp
colloidal gold ➢ Confirmation : Biochemical test
and/or serological test, oxidase test
4 Instrument Incubator 42 °C Incubator 37 °C Incubator 35 – 37 °C
5 Result Presumptive Positive : Presence of typical Salmonella is magenta- Salmonella spp →red color colonies,
Interpretation 1 line Negative red colonies. Coliforms → blue/ blue-violet
2 lines Positive other Enterobacteriaceae →
colourless/yellowish
* From various studies 97% to 99 % strains of
Confirmation : Salmonella give the typical red colonies
From secondary Enrichment, continue to except
2 Selective Agar plate accoding to ISO S. paratyphi A and S. typhi (colourless
6579:2002 colonies on Rambach® Agar).
6 Time 22-30 hrs for Low micobial spls Time release : 24-48 hours Incubation time 42-50 hrs + 1 hr wait
32-44 hrs for high microbial spls
Detection time test strip :
10 minutes
SDIX is the manufacturer of antibody test
strip
7 Inclusivity 100% specifity Can not detect / distinguish :
S.Typhi, S. Paratyphi, S. Rostock, S. Wassenar,
S.marina, S.wayne,
Serovar of S.enterica ssp. S.arizonae,
S.diarizonae. and serovar Salmonella that
produce -D-galactosidase such as S.bongori
8 Sensitivity 1 CFU/ 25 gr samples No data Sensitivity 93,9%
9 Approval AOAC PTM (#080601) No data AFNOR, only International research