Manual Técnico PENTRA 60 (2020 - 08 - 20 02 - 50 - 44 UTC)

Technical manual
HORIBA ABX SAS

Parc Euromédecine - Rue du Caducée
B.P. 7290
34184 MONTPELLIER Cedex 4 - FRANCE
P/n: RAA016CEN
Contents
1 - Introduction
1. Revision ..................................................................................................................2
2. Legal information ..................................................................................................3
3. Operational conditions .........................................................................................5
4. Environmental protection .....................................................................................7
5. Specifications ........................................................................................................8
Technical Manual
RAA045AEN 0-1
Contents
2 - Hydraulic & pneumatic principles
1. Hydropneumatic connections ..............................................................................2
2. Function of valves .................................................................................................8
3. Pneumatic diagrams .............................................................................................9
Technical Manual
0-2 RAA045AEN
Contents
3 - Electric & electronic principles
1. Main board test points ..........................................................................................2
2. Boards ....................................................................................................................4
3. Flat cable list .........................................................................................................8
4. Connections ...........................................................................................................9
5. Synoptic ...............................................................................................................25
Technical Manual
RAA045AEN 0-3
Contents
4 - Analysis cycle technology
1. Analysis cycle description ....................................................................................2
2. Measuring principles ............................................................................................8
3. Raw counts ..........................................................................................................17
Technical Manual
0-4 RAA045AEN
Contents
5 - Software
1. Versions .................................................................................................................2
2. USER menu overview ...........................................................................................3
3. MAINTENANCE menu overview ...........................................................................4
4. SETUP menu overview .........................................................................................5
5. TECHNICIAN menu overview ...............................................................................6
Technical Manual
RAA045AEN 0-5
Contents
6 - Alarm & error list
1. Printer .....................................................................................................................2
2. Transmission .........................................................................................................2
3. Calibration .............................................................................................................2
4. Technical Operations ............................................................................................3
5. Reagents ................................................................................................................3
6. Temperature ..........................................................................................................3
Technical Manual
0-6 RAA045AEN
Contents
7 - Maintenance
1. Maintenance ..........................................................................................................4
2. Procedures ............................................................................................................7
RAS235C: Installation
RAS236C: 6 month maintenance
RAS237C: Yearly maintenance
RAS238C: Every 2 years maintenance
RAS239C: Decontamination & rinse
RAS240C: LMNE flowcell adjustment
RAS241C: Chambers adjustment
RAS242C: Needle adjustment
RAS243C: Main board adjustment
RAS244C: Temperature adjustment
RAS245C: Vacuum adjustment
RAS247C: Covers dismantling
RAS248C: Heater assy replacement
RAS249C: Bubbling adjustment
RAS250C: Power supply replacement
RAS251C: Start cycle microswitch replacement
RAS252C: Check up after intervention
RAS253C: Step by step analysis cycle
RAS254C: Optical bench dismantling & replacement
RAS255C: External barcode reader configuration
RAS304C: LMNE balance adjustment
RAS680A: Takasago valve assembly dismantling
Technical Manual
RAA045AEN 0-7
Contents
8 - Exploded views
1. List ..........................................................................................................................2
2. Valves .....................................................................................................................6
3. Takasago valve assembly ....................................................................................7
4. 5 Diff syringe ..........................................................................................................8
5. Sampling syringe ...................................................................................................9
6. Reagents syringe ................................................................................................10
7. Waste syringe ......................................................................................................11
8. Counting syringe .................................................................................................12
9. Diluent tank assy .................................................................................................13
10. Rinse block & needle ........................................................................................14
11. Carriage assy .....................................................................................................15
12. Carriage peripherals .........................................................................................16
13. Optical bench ....................................................................................................17
14. Chambers for instrument NON equipped with Takasago valves .................18
15. Chambers for instrument equipped with Takasago valves ..........................19
16. Chamber parts ...................................................................................................20
17. Thermostated room ..........................................................................................21
18. Electronic boards ..............................................................................................22
19. Straws & tubes ..................................................................................................23
20. Reagent connection assembly ........................................................................24
21. Miscellaneous ....................................................................................................24
22. Power supply .....................................................................................................25
Technical Manual
0-8 RAA045AEN
Contents
23. Covers ................................................................................................................26
Technical Manual
RAA045AEN 0-9
Contents
Technical Manual
0 - 10 RAA045AEN
Introduction
1 - Introduction
1. Revision ..................................................................................................................2
2. Legal information ..................................................................................................3

2.1. Declaration of Conformity ..................................................................................................... 3
2.2. Notice of liability ................................................................................................................... 3
2.3. Document symbols ............................................................................................................... 3
2.4. Graphics ............................................................................................................................... 3
2.5. Trademarks ........................................................................................................................... 4
2.6. Copyright ® 2010 HORIBA ABX SAS ................................................................................... 4
3. Operational conditions .........................................................................................5

3.1. Environment .......................................................................................................................... 5
3.2. Location ................................................................................................................................ 5
3.3. Grounding ............................................................................................................................. 5
3.4. Humidity/temperature conditions ......................................................................................... 6
3.5. Electromagnetic environment check .................................................................................... 6
3.6. Main supply .......................................................................................................................... 6
4. Environmental protection .....................................................................................7

4.1. Used accessories and consumables disposal ..................................................................... 7
4.2. Instrument disposal .............................................................................................................. 7
4.3. European Legislation ............................................................................................................ 7
4.4. Transportation and storage conditions ................................................................................ 7
5. Specifications ........................................................................................................8
5.1. Technical specifications ....................................................................................................... 8
5.2. Physical specifications ....................................................................................................... 11
Technical Manual
RAA016CEN 1-1
Introduction
Revision
1. Revision
Index P/n revision Revision Section Date

RAA016A Creation All 19/07/99
RAA016B Update All 25/05/04
RAA016CEN ECR 4284 C All 10/2011
This document applies to the latest software listed and higher versions.
When a subsequent software version changes the information in this document, a new electronic issue
(CD-ROM and/or online help) is released and supplied by HORIBA Medical.
To update a paper document, please contact your local HORIBA Medical representative.
Technical Manual
1-2 RAA016CEN
Introduction
Legal information
2. Legal information
2.1. Declaration of Conformity
This instrument responds to the Standards and Directives named in the Declaration of Conformity.
Latest version of the CE Declaration of conformity for this instrument is available on www.horiba-
abx.com
2.2. Notice of liability
The Information in this manual is distributed on an "As Is" basis, without warranty. While every
precaution has been taken in the preparation of this manual, HORIBA Medical will not assume any
liability to any persons or entities with respect to loss or damage, caused or alleged to be caused directly
or indirectly by not following the instructions contained in this manual, or by using the computer software
and hardware products described herein in a manner inconsistent with our product labelling.
2.3. Document symbols
To alert the operator of potentially hazardous conditions, one of the headings which are described below
is provided wherever necessary throughout this text.
Emphasizes information that must be followed to avoid hazard to either the operator or the
environment, or both.
Emphasizes information that must be followed to avoid possible damage to the instrument
or erroneous test results.
Emphasizes information that can be helpful to the operator before, during or after a specific
operational function.
2.4. Graphics
All graphics including screens and printouts, photographs are for illustration purposes only and are not
contractual.
Technical Manual
RAA016CEN 1-3
Introduction
Legal information
2.5. Trademarks
Product names mentioned within this publication may be trademarks or registered trademarks of their
respective owners.
2.6. Copyright ® 2010 HORIBA ABX SAS
All rights reserved. No part of this book may be reproduced or transmitted in any form or by any means,
electronic, mechanical, photocopying, recording, or otherwise, without the prior written permission of
HORIBA Medical.
HORIBA ABX SAS

Rue du Caducée - Parc Euromédecine
34184 MONTPELLIER Cedex 4 - FRANCE
Tel: + 33 (0)4 67 14 15 16
Fax: + 33 (0)4 67 14 15 17
Technical Manual
1-4 RAA016CEN
Introduction
Operational conditions
3. Operational conditions
3.1. Environment
■ The operation of the Pentra 60 should be restricted to indoor location use only. Instrument is
operational at an altitude of maximum 3000 meters (9840 feet).
■ The Pentra 60 is designed for safety from voltages surges according to INSTALLATION CATEGORY II
and POLLUTION DEGREE 2 (IEC 61010-1).
■ Please contact your local HORIBA Medical representative for information regarding operation
locations, when it does not comply with the recommended specifications.
3.2. Location
■ The Pentra 60 should be placed on a clean and leveled table or workbench.

■ Please note that the instrument and reagents weigh approximately 35 kilograms (77 lbs).
■ Avoid exposure to sunlight.
■ Place your instrument in a well-ventilated area.
■ Place your instrument where it is not exposed to water or vapor.
■ Place your instrument where it is free from vibration or shock.
■ Place your instrument where an independent power receptacle can be used.
■ Use a receptacle different from the one used by a device that easily generate noise such as a
centrifuge, etc...
■ Provide a space of at least 20 cm (8 inches) at the back of the instrument for arranging the power cable
and tubings.
The power switch and input voltage supply connection should always be accessible. When
positioning the system for operational use, leave the required amount of space for easy
accessibility to these items.
3.3. Grounding
Proper grounding is required when installing the system. Check the wall outlet ground (Earth) for proper
grounding to the facilities electrical ground. If you are unsure of the outlet grounding, contact your
facilities engineer to verify the proper outlet ground.
Technical Manual
RAA016CEN 1-5
Introduction
Operational conditions
3.4. Humidity/temperature conditions
■ The Pentra 60 must operate between temperatures of 16°C to 34°C (61°F to 93°F).
■ Maximum relative humidity 80% for temperature up to 31°C (88°F) without condensation.
■ If it is stored at a temperature less than 10°C (50°F), the instrument should stand for 1 hour at the
correct room temperature before use.
3.5. Electromagnetic environment check
■ The Pentra 60 has been designed to produce less than the accepted level of electromagnetic
interference in order to operate in conformity with its destination, allowing the correct operation of
other instruments also in conformity with their destination.
■ In case of suspected electromagnetic noise, check that the instrument has not been placed in the
proximity of electromagnetic fields or short wave emissions, (i. e. Radar, X-rays, Scanners, Cell
phones, etc...).
3.6. Main supply
■ Grounding is required. Check that the earth wall-plug is correctly connected to the laboratory
grounding system. If there is no such system a ground stake should be used.
■ Use only main supply cable delivered with the Pentra 60.
■ Main supply voltage fluctuations must not exceed +/-10% of the nominal voltage.
Technical Manual
1-6 RAA016CEN
Introduction
Environmental protection
4. Environmental protection
4.1. Used accessories and consumables disposal
Disposable used accessories and consumables must be collected by a laboratory specialized in

elimination and recycling of this kind of material according to the local legislation.
4.2. Instrument disposal
It should be disposed of, in accordance with local legislation, and should be treated as being
contaminated with blood. The appropriate biological precautions should be taken.
If any doubt, please contact your HORIBA Medical representative service department.
4.3. European Legislation
■ In accordance with the European Directive (2002/96/CE, known also as W.E.E.E)

instruments having the above symbol and sold into a European country by HORIBA
Medical or an authorised representative must be disposed of and recycled correctly at the
end of its useful life.
■ Due to the local changing regulations in each country, please contact your local
representative for detailed and up to date information on how to appropriately dispose of
the instrument.
4.4. Transportation and storage conditions
Condition for storage and transportation: Temperature from -20°C to +50°C (-4°F to 122°F).
Prior to the shipping of an instrument by transporter, whatever the destination, an external

decontamination of the instrument must be carried out.
Technical Manual
RAA016CEN 1-7
Introduction
Specifications
5. Specifications
5.1. Technical specifications
5.1.1. Intended use

■ The Pentra 60 is a fully automated hematology analyzer used for the in vitro diagnostic testing of whole
blood specimens.
■ The Pentra 60 is able to operate either in CBC mode (Cell Blood Count:12 parameters) or in CBC +
5DIFF mode (5 population differential count: 26 parameters).
5.1.2. Parameters
CBC mode
Parameters Descriptions
WBC White blood cells
RBC Red blood cells
HGB Hemoglobin concentration
HCT Hematocrit
MCV Mean corpuscular volume
MCH Mean corpuscular Hemoglobin
MCHC Mean corpuscular Hemoglobin concentration
RDW Red distribution width
PLT Platelets
PDW* Platelets distribution width
PCT* Plateletcrit
MPV Mean platelet volume
* PDW and PCT have not been established as indications for this product, in the United
States. The use of PCT and PDW should be restricted to research and Investigational
measurements only.
CBC + 5DIFF mode

WBC White blood cells
LYM # Lymphocytes #
LYM% Lymphocytes %
MON # Monocytes #
MON % Monocytes %
NEU # Neutrophils #
NEU % Neutrophils %
EOS # Eosinophils #
EOS % Eosinophils %
BAS # Basophils #
Technical Manual
1-8 RAA016CEN
Introduction
Specifications
CBC + 5DIFF mode

BAS % Basophils %
ALY # Atypical Lymphocyte #
ALY % Atypical Lymphocyte %
LIC # Large Immature Cell #
LIC % Large Immature Cell %
RBC Red blood cells
HGB Hemoglobin concentration
HCT Hematocrit
MCV Mean corpuscular volume
MCH Mean corpuscular Hemoglobin
MCHC Mean corpuscular Hemoglobin concentration
RDW Red distribution width
PLT Platelets
PDW* Platelets distribution width
PCT* Plateletcrit
MPV Mean platelet volume
* PDW and PCT have not been established as indications for this product, in the United
States. The use of PCT and PDW should be restricted to research and Investigational
measurements only.
5.1.3. Units
Parameters Standard SI mmol Japanese

WBC 103/mm3 109/l 109/l 102/mm3
RBC 106/mm3 1012/l 1012/l 104/mm3
HGB g/dl g/l mmol/l g/dl
HCT % l/l l/l %
MCV µm3 fl fl µm3
MCH pg pg fmol pg
MCHC g/dl g/l mmol/l g/dl
RDW % % % %
PLT 103/mm3 109/l 109/l 104/mm3
PDW* % % % %
PCT* % 10-2/l 10-2/l %
MPV µm3 fl fl µm3
LYM # 103/mm3 109/l 109/l 102/mm3
LYM% % % % %
MON # 103/mm3 109/l 109/l 102/mm3
MON % % % % %
NEU # 103/mm3 109/l 109/l 102/mm3
NEU % % % % %
EOS # 103/mm3 109/l 109/l 102/mm3
EOS % % % % %
BAS # 103/mm3 109/l 109/l 102/mm3
Technical Manual
RAA016CEN 1-9
Introduction
Specifications
Parameters Standard SI mmol Japanese

BAS % % % % %
ALY #* 103/mm3 109/l 109/l 102/mm3
ALY %* % % % %
LIC #* 103/mm3 109/l 109/l 102/mm3
LIC %* % % % %
5.1.4. Throughput analyses

■ CBC mode (CBC): 60/h
■ CBC + 5DIFF mode (DIF): 60/h
5.1.5. Tube identification

■ By means of the worklist or identification file.
■ By means of the Barcode labels
5.1.6. Reagents
■ ABX Diluent (10 or 20 l)
■ ABX Cleaner (1 l, integrated)
■ ABX Eosinofix (1 l, integrated)
■ ABX Basolyse 2 (1 l, integrated)
■ ABX Alphalyse or ABX Lysebio ( 0,4 l, integrated)
The CD-ROM RAX055 delivered with your instrument provides Reagents, Controls and
Calibrators leaflets/msds. Latest versions of these documents are available on www.horiba-
abx.com/documentation.
5.1.7. Measurement & computation

■ Impedance for WBC, BASO, RBC, PLT.
■ Photometry for HGB.
■ Impedance and light scattering for LYM, MON, NEU, EOS, ALY and LIC.
■ Computation from stored data that was directly measured for HCT, MCV, MCH, MCHC, RDW, MPV,
PCT, PDW.
Technical Manual
1 - 10 RAA016CEN
Introduction
Specifications
5.2. Physical specifications
5.2.1. Power requirements

■ Power supply: from 100 Vac to 240 Vac (+/- 10%), 50 Hz to 60 Hz
■ Analyzer power consumption: 200 W
■ Printer: depends on printer (see printer’s manual)
5.2.2. Dimensions & weight

■ Instrument dimensions:
- Height: approximately 516 mm (20.3 in.)
- Width: approximately 444 mm (17.5 in.)
- Depth: approximately 481 mm (19 in.)
■ Instrument weight: 35 kilograms (77 lbs)
5.2.3. Minimum specimen volume

■ CBC mode (CBC): 30 µl
■ CBC + 5DIFF mode (DIF): 53 µl
5.2.4. Dilution ratios

■ WBC/BASO: 1/200
■ LMNE: 1/80
■ RBC/PLT: 1/10000
■ HGB: 1/250
5.2.5. HGB measurement

■ HGB chamber, LED 555 nm.
■ ModifiedDrabkin method (cyanmethemoglobin for ABX Alphalyse) or cyanide-free determination (for
ABX Lysebio).
■ Light source: Electroluminescent diode.
■ Wavelength: 550 nm +/- 10 nm
5.2.6. Counting aperture diameters

■ WBC/BASO: 80 µm
■ LMNE: 60 µm
■ RBC/PLT: 50 µm
Technical Manual
RAA016CEN 1 - 11
Introduction
Specifications
Technical Manual
1 - 12 RAA016CEN
Hydraulic & pneumatic principles
2 - Hydraulic & pneumatic principles
1. Hydropneumatic connections ..............................................................................2

1.1. For Pentra 60 NON equipped with Takasago valves ........................................................... 2
1.2. For Pentra 60 equipped with Takasago valves .................................................................... 5
2. Function of valves .................................................................................................8
3. Pneumatic diagrams .............................................................................................9
Technical Manual
RAA016CEN 2-1
Hydropneumatic connections
1. Hydropneumatic connections
1.1. For Pentra 60 NON equipped with Takasago valves
Read this table as follows: LV3_2«S» means connection to port 2 of valve 3 with a «Sleeving».
Circuit From Diameter Length To

ABX Cleaner
ABX Cleaner bottle 2.06 400 LV7_1
LV7_3 2.06 120 Reagent Syringe_2
LV7_2 1.52 230 LV12_3
LV12_1 1.52 350 WBC/BASO chamber_1
LV12_2 1.52 280 T3_3
ABX Eosinofix
ABX Eosinofix bottle 2.06 400 LV8_1
LV8_2 1.52 500 Reagent Heater_3«S»
Reagent Heater_4«S» 1.52 120 LMNE chamber_3
ABX Basolyse 2
ABX Basolyse 2 bottle 2.06 400 LV11_1
LV11_3 2.06 15 E3 Straight connector
E3 Straight connector 2.06 120 Reagent Syringe_5
E4 Straight connector 2.06 500 Reagent Heater_10«S»
Reagent Heater_9«S» 2.06 60 Reagent Heater_11«S»
Reagent Heater_12«S» 2.06 120 E5 Straight connector
E5 Straight connector 1.52 15 WBC/BASO chamber_2
ABX Lyse
ABX Lyse bottle 1.52 350 LV6_1
LV6_2 1.52 480 DIL1/HGB chamber_2
Sampling
Needle_1 1.02 205 Sampling Syringe_1
LMNE counting
LMNE chamber_4 1.30 20 M1_2 photocell
M1_1 photocell 1.30 320 LV4_1
LV4_2 1.02 130 T2_2
T2_3 1.02 240 LMNE Syringe_5
LMNE Syringe_3 1.02 95 LV5_1
LV5_2 2.06 420 T6_2
T2_1«S» 1.5 10 LMNE flowcell_6
0.64 9 LMNE flowcell_6
LMNE flowcell_Output 1.52 20 E1_1 Anode fitting
Technical Manual
2-2 RAA016CEN

E1_2 Anode fitting 1.52 70 Isolator_1
Isolator_2 1.52 80 E2_1 Ground fitting
E2_2 Ground fitting 1.52 335 LV24_2
LV24_1 1.52 120 T4_2
T4_1 1.52 160 Reagent Heater_5«S»
ABX Diluent
Diluent Input 2.54 170 LV25_2«S»
LV25_1«S» 2.54 140 Diluent Tanker_4«S»
2.06 65 Diluent Tanker_1
Diluent Tanker_1 2.06 350 LV3_1
LV3_3«S» 2.06 120 LMNE Syringe_2
LV3_2«S» 1.52 50 LV2_3«S»
LV2_1«S» 1.52 50 LV1_3«S»
LV1_1«S» 1.52 200 Isolator_1«S»
Isolator_2«S» 1.52 100 T1_1
T1_2 1.14 35 LMNE flowcell_4
T1_2 XBA403 LMNE flowcell_4
LV1_2 1.52 250 T4_3
LV2_2 1.02 175 LMNE Syringe_4
LMNE Syringe_1 1.02 205 LMNE flowcell_5
LMNE flowcell_7 1.02 10 (Cap)
LV9_2 1.52 50 LV10_3
LV10_1 1.52 15 T3_2
T3_1 1.52 640 LV18_3
LV18_1 1.52 330 Needle rinsing block_1
Needle rinsing block_2 1.52 300 LV17_1
LV17_2 1.52 820 T5_2
LV18_2 1.02 110 Sampling Syringe_2
LV10_2 1.52 380 LV21_3
LV21_1 1.52 50 LV22_3
Reagent Heater_8«S» 1.52 120 RBC chamber_1
Reagent Heater_2«S» 1.52 120 DIL1/HGB chamber_1
WBC/RBC Counting
WBC/BASO chamber_3 1.52 200 LV23_3
LV23_1 1.52 200 RBC chamber_3
RBC chamber_2 1.52 430 LV14_2
Technical Manual
RAA016CEN 2-3

LV14_1 1.52 70 Counting Syringe_2
DIL1/HGB chamber_4 1.02 50 LV34_1
Drainings
Rinse chamber_3 2.06 100 LV27_2
LV27_1 2.06 30 T7_3
LV28_1 2.06 20 T7_2
T7_1 2.06 20 T8_3
LMNE chamber_5 2.06 70 LV29_2
LV29_1 2.06 20 T8_2
T8_1 2.06 20 T9_3
LV30_1 2.06 20 T9_2
T9_1 2.06 20 T10_2
LV31_1 2.06 20 T10_1
T10_3 2.06 30 T6_3
T6_1 2.06 20 T5_3
T5_1 2.06 50 M2_1 photocell
M2_2 photocell 2.06 15 Isolator_1
Isolator_2 2.06 280 LV26_1
LV26_3«S» 2.54 150 Draining Syringe_2«S»
LV26_2«S» 2.54 130 T11_3
Counting Syringe_5 2.06 125 LV16_1
LV16_2«S» 2.54 340 T11_2
T11_1 2.54 20 Waste Output
Air
Atmosphere 2.06 140 LV13_2
LV15_3 1.52 400 Diluent Tanker_2
Rinse_2 chamber (Atmo.) 2.06 300 LV20_2
LV20_1 1.52 90 Draining Syringe_1
Technical Manual
2-4 RAA016CEN
1.2. For Pentra 60 equipped with Takasago valves
Read this table as follows: LV3_2«S» means connection to port 2 of valve 3 with a «Sleeving».

ABX Cleaner
ABX Cleaner bottle 2.06 400 LV7_1
LV7_2 1.52 230 LV12_3
LV12_2 1.52 280 T3_3
ABX Eosinofix
ABX Eosinofix bottle 2.06 400 LV8_1
ABX Basolyse 2
ABX Basolyse 2 bottle 2.06 400 LV11_1
E3 Straight connector 2.06 120 Reagent Syringe_5
E4 Straight connector 2.06 500 Reagent Heater_10«S»
Reagent Heater_9«S» 2.06 60 Reagent Heater_11«S»
Reagent Heater_12«S» 2.06 120 E5 Straight connector
E5 Straight connector 1.52 15 WBC/BASO chamber_2
ABX Lyse
ABX Lyse bottle 1.52 350 LV6_1
LV6_2 1.52 480 DIL1/HGB chamber_2
Sampling
Needle_1 1.02 205 Sampling Syringe_1
LMNE counting
LMNE chamber_4 1.30 20 M1_2 photocell
M1_1 photocell 1.30 320 LV4_1
LV4_2 1.02 130 T2_2
T2_3 1.02 240 LMNE Syringe_5
LMNE Syringe_3 1.02 95 LV5_1
LV5_2 2.06 420 T6_2
T2_1«S» 1.5 10 LMNE flowcell_6
0.64 9 LMNE flowcell_6
LMNE flowcell_Output 1.52 20 E1_1 Anode fitting
E1_2 Anode fitting 1.52 70 Isolator_1
Isolator_2 1.52 80 E2_1 Ground fitting
E2_2 Ground fitting 1.52 335 LV24_2
LV24_1 1.52 120 T4_2
Technical Manual
RAA016CEN 2-5

T4_1 1.52 160 Reagent Heater_5«S»
ABX Diluent
Diluent Input 2.54 170 LV25_2«S»
LV25_1«S» 2.54 140 Diluent Tanker_4«S»
LV3_3«S» 2.06 120 LMNE Syringe_2
LV3_2«S» 1.52 50 LV2_3«S»
LV2_1«S» 1.52 50 LV1_3«S»
LV1_1«S» 1.52 200 Isolator_1«S»
Isolator_2«S» 1.52 100 T1_1
LV1_2 1.52 250 T4_3
LV2_2 1.02 175 LMNE Syringe_4
LMNE Syringe_1 1.02 205 LMNE flowcell_5
LMNE flowcell_7 1.02 10 (Cap)
LV9_2 1.52 50 LV10_3
LV10_1 1.52 15 T3_2
T3_1 1.52 640 LV18_3
LV18_1 1.52 330 Needle rinsing block_1
Needle rinsing block_2 1.52 300 LV17_1
LV17_2 1.52 820 T5_2
LV18_2 1.02 110 Sampling Syringe_2
LV10_2 1.52 380 LV21_3
LV21_1 1.52 50 LV22_3
Reagent Heater_8«S» 1.52 160 T12_3
Reagent Heater_2«S» 1.52 120 DIL1/HGB chamber_1
WBC/RBC Counting
LV34_2 LV33_2
Technical Manual
2-6 RAA016CEN

LV33_1 1.52 180 T12_2
T12_1 1.52 70 LV34_3
Drainings
Rinse chamber_3 2.06 100 LV27_2
LV27_1 2.06 30 T7_3
LV28_1 2.06 20 T7_2
T7_1 2.06 20 T8_3
LMNE chamber_5 2.06 70 LV29_2
LV29_1 2.06 20 T8_2
T8_1 2.06 20 T9_3
LV30_1 2.06 20 T9_2
T9_1 2.06 20 T10_2
LV31_1 2.06 20 T10_1
T10_3 2.06 30 T6_3
T6_1 2.06 20 T5_3
T5_1 2.06 50 M2_1 photocell
M2_2 photocell 2.06 15 Isolator_1
Isolator_2 2.06 280 LV26_1
LV26_3«S» 2.54 150 Draining Syringe_2«S»
LV26_2«S» 2.54 130 T11_3
Counting Syringe_5 2.06 125 LV16_1
LV16_2«S» 2.54 340 T11_2
T11_1 2.54 20 Waste Output
Air
LV15_3 1.52 400 Diluent Tanker_2
Rinse_2 chamber (Atmo.) 2.06 300 LV20_2
LV20_1 1.52 90 Draining Syringe_1
Technical Manual
RAA016CEN 2-7
Function of valves
2. Function of valves
Valve # Valve subject Valve function

1 DIL LMNE Directing optical chamber / LMNE chamber
2 DIL LMNE Directing Internal / External sleeving
3 DIL LMNE Control Entry / Exit syringe
4 LMNE Transfer control LMNE tray /Injection syringe
5 LMNE Transfer control LMNE tray /Injection syringe
6 Lyse Control Entry / Exit syringe
7 Cleaner Control Entry / Exit syringe
8 Eosinofix Control Entry / Exit syringe
9 Dil Control Entry / Exit syringe
10 Dil Directing Circuit chamber / Needle circuit
11 Basolyse 2 Control Entry / Exit syringe
12 Cleaner Directing WBC/BASO chamber / Needle circuit
13 Counting syringe Set in the atmosphere
14 Counting syringe RBC/PLT count
15 Tank Set in the atmosphere
16 Counting syringe Draining
17 Needle Flushing out of rinsing unit
18 Needle Commutation Diluent interior/exterior Needle
20 Draining syringe Set in the atmosphere
21 Dil Directing head for WBC count / Valve 22
22 Dil Directing diluent chamber dil.1
23 Counting syringe WBC/RBC selection count
24 LMNE Exit from the optical chamber
25 Diluent tank Entry of the diluent
26 Draining syringe Draining of the syringe
27 Chamber group Drain rinse chamber
28 Chamber group Drain first dilution chamber
29 Chamber group Drain LMNE chamber
30 Chamber group Drain RBC/PLT chamber
31 Chamber group Drain WBC/BASO chamber
33* RBC Dilution Select and transfer a sample from the first dilution for RBC counting
34* RBC Dilution Select and transfer a sample from the first dilution for RBC counting
*: Instruments equipped with Takasago valves only.
Technical Manual
2-8 RAA016CEN
Pneumatic diagrams
3. Pneumatic diagrams
See pneumatic diagrams on next page
Technical Manual
RAA016CEN 2-9
Pneumatic diagrams
Technical Manual
2 - 10 RAA016CEN
Electric & electronic principles
3 - Electric & electronic principles
1. Main board test points ..........................................................................................2
2. Boards ....................................................................................................................4
2.1. XAA423B, Optical Preampli board ....................................................................................... 4
2.2. XAA425C, Main board .......................................................................................................... 5
2.3. XAA427B, Motor relay board ................................................................................................ 6
2.4. XAA428B, Carriage board .................................................................................................... 7
2.5. XAA429A, LED board ........................................................................................................... 7
3. Flat cable list .........................................................................................................8
4. Connections ...........................................................................................................9
4.1. List ........................................................................................................................................ 9
4.2. XBA193A, Coax BASO-O.D LMNE-LMNE ........................................................................... 9
4.3. XBA387A, Reagent heating coil harness ............................................................................ 10
4.4. XBA389A, Photometer HGB ............................................................................................... 11
4.5. XBA390A, Motor for syringe blocks ................................................................................... 11
4.6. XBA391B, Carriage motor .................................................................................................. 11
4.7. XBA392A, Sampling needle motor ..................................................................................... 11
4.8. XBA393A, Main fan 24V ..................................................................................................... 12
4.9. XBA394A, Carriage sensor ................................................................................................. 13
4.10. XBA396A, Sampling needle sensor .................................................................................. 14
4.11. XBA398B, RBC/WBC coaxial cable ................................................................................. 15
4.12. XBA399C, Ground flowcell fitting ..................................................................................... 16
4.13. XBA402A, Barcode reader harness .................................................................................. 17
4.14. XBA458A, Syringe switch cable Lg=300 mm ................................................................... 18
4.15. XBA460A, Syringe switch cable Lg=500 mm ................................................................... 18
4.16. XBA462A, Motor cable Lg=300 mm ................................................................................. 19
4.17. XBA480A, Optical preampli board harness ...................................................................... 20
4.18. XBA481A, LED board harness .......................................................................................... 20
4.19. XBA482A, Door security switch harness .......................................................................... 21
4.20. XBA620A, Draining detection sensor ............................................................................... 21
4.21. XBA645A, Takasago valve extension harness ................................................................. 22
4.22. XBA646A, Takasago valve wired ...................................................................................... 23
4.23. XDA605A, Diluent tank assembly ..................................................................................... 24
4.24. XDA812A, T° room fan + harness ..................................................................................... 24
5. Synoptic ...............................................................................................................25
Technical Manual
RAA016CEN 3-1
Main board test points
1. Main board test points
Test
Designation Potentiometer Target Tolerance Comment
point
TP1 HGB Blank adjust. R248 4.7V +/-0.02 V
TP2 PLT Threshold adjust. R159 300 mV +/-5 mV
TP3 LMNE CIS Threshold adjust. R160 650 mV +/-5 mV
TP4 LMNE OD Threshold adjust. R161 350 mV +/-5 mV
TP5 Draining syringe motor R149 4.8V +/-0.05V
TP6 Counting syringe motor R150 4.8V +/-0.05V
TP7 Reagent syringe motor R151 4.0 V +/-0.05V
TP8 LMNE syringe motor R152 3.0 V +/-0.05V
TP9 Unused
TP10 Horizontal carriage motor R154 3.0 V +/-0.05V
TP11 Sampling syringe motor R155 2.0 v +/-0.05V
TP12 Needle carriage motor R156 4.5 V +/-0.05V
TP13 RBC Threshold adjust. R158 300 mV +/-5 mV
TP14 BASO Threshold adjust. R157 300 mV +/-5 mV
TP15 BASO Comparator
TP16 RBC Comparator
TP17 PLT Comparator
TP18 LMNE CIS Comparator
TP19 LMNE OD Comparator
TP20 RBC line height adjust. R133 Factory Adjusted
TP21 BASO gain adjust. R134 Factory Adjusted
TP22 PLT line height adjust. R135 Factory Adjusted
TP23 LMNE Resistive gain adjust. R136 Factory Adjusted
TP24 LMNE Optical gain adjust. R148 Factory Adjusted
TP25 RBC line width adjust.
TP26 RBC line reject adjust. R142 10 µs +/- 0.5µs Factory Adjusted
TP27 RBC line pulse adjust. Factory Adjusted
TP28 BASO line width adjust.
TP29 BASO line reject adjust. R139 10 µs +/- 0.5µs Factory Adjusted
TP30 BASO line pulse adjust. Factory Adjusted
TP31 PLT line width adjust.
TP32 PLT line reject adjust. R144 10 µs +/- 0.5µs Factory Adjusted
TP33 PLT line pulse adjust. Factory Adjusted
TP34 LMNE Res. line width adjust.
TP35 LMNE Res. line reject adjust. R145 15 µs +/- 0.5µs Factory Adjusted
TP36 LMNE Res. line pulse adjust Factory Adjusted
TP37 LMNE Opt. line width adjust.
TP38 LMNE Opt. line reject adjust. R147 5 µs +/- 0.5µs Factory Adjusted
TP39 LMNE Opt. line pulse adjust. Factory Adjusted
TP40 5 V Power supply 5V +/- 0.15 V Non adjustable
Technical Manual
3-2 RAA016CEN
Main board test points
Test
Designation Potentiometer Target Tolerance Comment
point
TP41 12 V Power supply 12 V +/- 0.5 V Non adjustable
TP42 24 V Power supply 24 V +/- 1.4 V Non adjustable
TP43 -12 V Power supply -12 V +/- 0.5 V Non adjustable
TP44 GROUND
TP45 GROUND
TP46 GROUND
TP47 GROUND
TP48 LMNE Draining sensor R286 4.5 V +/- 0.3 V On air
<1 V With water
TP49 Unused
Reagent heating system CTN
TP50
voltage
Thermostated room heating
TP51
sytem CTN voltage
TP52 Draining sensor R287 4.5 V +/- 0.3 V On air
<1 V With water
TP53 LMNE Lamp R11 6V
TP54 GROUND
TP55 GROUND
TP56 GROUND
TP57 GROUND
TP58 GROUND
TP59 GROUND
LMNE Res. line reject 2
TP60 R409 50 µs +/- 2 µs Factory Adjusted
adjust.
LMNE Res. line reject 2
TP61 R410 250 µs +/- 5 µs Factory Adjusted
adjust.
Technical Manual
RAA016CEN 3-3
Boards
2. Boards
2.1. XAA423B, Optical Preampli board
Adjustment of LMNE lamp voltage

LMNE lamp voltage between TP1 and TP2
(6,0 V +/-0,5)
Coaxial cable (XBA193A)

to J25 on Main board
XBA480A (+ 12V)
to J36 on Main board
■ Perform an absorbance measurement.

■ This is an Intensity / Tension convertissor.
■ A photodiode measures absorbance of light through the cell, the optical preampli board returns an
intensity proportional to the light signal (light signal is proportional to the cell size).
Technical Manual
3-4 RAA016CEN
Boards
2.2. XAA425C, Main board
Coaxial cable to J1 Coaxial cable to RBC/PLT WBC/BASO Spectrophotometer

on optical preampli board LMNE flowcell counting head counting head (XBA389A)
Fan (XBA193A) (XBA193A) (XBA398B) (XBA398B)
(XBA393A)
DAD113A to
J2 on motor
relay board
DAD112A to
J5 on carriage
board + TAKASAGO
valve assy
Not used
DAD107A to
valves #1 - 6
DAD109A to
valves #7 - 11
DAD075A to
valves #20 - 26
DAD109A to
valves #27 - 31
DAD107A to
valves #12 - 16
Not used
Heater assy
XBA387A
Not used Room heater Not used Power supply Start cycle Not used Barcode
(XDA812A) switch (XBA482A) reader output
(XBA402A)
Draining sensor M8 Printer output Not used RS232 output

(XBA620A) (DAD111A) (DAD078A)
Door security LMNE transfer DAD110A to Keyboard & Display assy J2 on optical
switch (XBA482A) sensor M9 (XBA620A) (XAA444A) preampli board
(XBA480A)
Technical Manual
RAA016CEN 3-5
Boards
2.3. XAA427B, Motor relay board
XBA462A, B7 XBA458A, SW7

Drain syringe motor Drain syringe home switch

Count syringe motor Count syringe home switch

Reagent syringe motor Reagent syringe home switch

LMNE syringe motor LMNE syringe home switch
Not used XDA605A, Diluent reservoir
DAD113A to J20 on main board
XBA391B, B3 XBA394A, M3
Horizontal carriage motor Horizontal carriage home sensor
■ Interconnection board where all the different motors and end of run sensor are connected
■ This board is connected to the main board with a flat cable
Technical Manual
3-6 RAA016CEN
Boards
2.4. XAA428B, Carriage board
DAD112A to J1
XBA390A, B2
on Main board
Sample syringe motor
XBA392A, B1
Vertical carriage motor
XBA458A, SW2
Sample syringe home switch
XBA396A, M1
Vertical carriage
home sensor
■ Interconnection board where all the carriage electrical functions are connected
■ This board is connected to the main board with one flat cable
2.5. XAA429A, LED board
XBA421B to J9
on Main board
■ This board supports LEDs on the front panel. They give instrument state:
- Red: instrument is busy
- Green: instrument is ready to sample
Technical Manual
RAA016CEN 3-7
Flat cable list
3. Flat cable list
Length / number of
Reference From To
points
DAD075A 350 mm / 14 pts J18 on main board J1 on 7 valves assy
DAD078A 405 mm / 10 pts J4 on main board RS232 output
J15 on main board J1 on 5 valves assy
J14 on main board J1 on 5 valves assy (Non Takasago)
DAD136A J1 on 5 valves assy (Takasago)
DAD110A 750 mm / 40 pts J9 on main board J1 on Keyboard & LCD board
DAD111A 300 mm / 26 pts J2 on main board Printer output
DAD112A 930 mm / 26 pts J1 on main board J5 on carriage board
DAD113A 470 mm / 50 pts J20 on main board J2 on motor relay board
DAD136A 80 mm / 14 pts J14 on main board DAD109A and XBA645A (Takasago only)
Technical Manual
3-8 RAA016CEN
Connections
4. Connections
4.1. List
Reference From To
XBA193A J22 on main board LMNE flowcell
J25 on main board J1 on Optical preampli board
XBA387A J31 on main board Heating coil harness
XBA389A J26 on main board HGB photometer
XBA390A J4 on carriage board Sampling syringe motor (B2)
J11 on motor relay board Reagent syringe motor (B6)
XBA391B J14 on motor relay board Horizontal carriage motor (B3)
XBA392A J3 on carriage board Vertical carriage motor (B1)
XBA393A J35 on main board Fan
XBA394A J1 on motor relay board Horizontal carriage motor sensor (M3)
XBA396A J1 on carriage board Vertical carriage motor sensor (M1)
XBA398B J23 on main board RBC/PLT counting head
J24 on main board WBC/BASO counting head
XBA399C XBA193A on LMNE flowcell LMNE flowcell
XBA402A J5 on main board Barcode reader output
XBA421B J9 on main board J1 on LEDS board
XBA458A J2 on carriage board Sampling syringe motor home (SW2)
J7 on motor relay board Count syringe motor home (SW4)
J8 on motor relay board Drain syringe motor home (SW7)
XBA460A J5 on motor relay board LMNE syringe motor home (SW5)
J6 on motor relay board Reagent syringe motor home (SW6)
XBA462A J10 on motor relay board LMNE syringe motor (B5)
J12 on motor relay board Count syringe motor (B4)
J13 on motor relay board Drain syringe motor (B7)
XBA480A J36 on main board J2 on Optical preampli board
XBA482A J27 on main board Door security switch
J34 on main board Start cycle switch (Pentra MS60 only)
XBA620A J29 on main board Transfer sensor (M9)
J30 on main board Draining sensor (M8)
DAD136A (connected between J14 on main
XBA645A TAKASAGO valve assy XBA645A
board and DAD109A)
XBA646A XBA645A TAKASAGO valve assy
XDA605A J4 on motor relay board Diluent reservoir
XDA812A J33 on main board Chambers heating harness
4.2. XBA193A, Coax BASO-O.D LMNE-LMNE
No diagram available
Technical Manual
RAA016CEN 3-9
Connections
4.3. XBA387A, Reagent heating coil harness
Technical Manual
3 - 10 RAA016CEN
Connections
4.4. XBA389A, Photometer HGB
4.5. XBA390A, Motor for syringe blocks
4.6. XBA391B, Carriage motor
4.7. XBA392A, Sampling needle motor
Technical Manual
RAA016CEN 3 - 11
Connections
4.8. XBA393A, Main fan 24V
Technical Manual
3 - 12 RAA016CEN
Connections
4.9. XBA394A, Carriage sensor
Technical Manual
RAA016CEN 3 - 13
Connections
4.10. XBA396A, Sampling needle sensor
Technical Manual
3 - 14 RAA016CEN
Connections
4.11. XBA398B, RBC/WBC coaxial cable
Technical Manual
RAA016CEN 3 - 15
Connections
4.12. XBA399C, Ground flowcell fitting
Technical Manual
3 - 16 RAA016CEN
Connections
4.13. XBA402A, Barcode reader harness
Technical Manual
RAA016CEN 3 - 17
Connections
4.14. XBA458A, Syringe switch cable Lg=300 mm
4.15. XBA460A, Syringe switch cable Lg=500 mm
Technical Manual
3 - 18 RAA016CEN
Connections
4.16. XBA462A, Motor cable Lg=300 mm
Technical Manual
RAA016CEN 3 - 19
Connections
4.17. XBA480A, Optical preampli board harness
4.18. XBA481A, LED board harness
Technical Manual
3 - 20 RAA016CEN
Connections
4.19. XBA482A, Door security switch harness
4.20. XBA620A, Draining detection sensor
Technical Manual
RAA016CEN 3 - 21
Connections
4.21. XBA645A, Takasago valve extension harness
Technical Manual
3 - 22 RAA016CEN
Connections
4.22. XBA646A, Takasago valve wired
Technical Manual
RAA016CEN 3 - 23
Connections
4.23. XDA605A, Diluent tank assembly
4.24. XDA812A, T° room fan + harness
Technical Manual
3 - 24 RAA016CEN
Synoptic
5. Synoptic
See synoptics on following page.
Technical Manual
RAA016CEN 3 - 25
Synoptic
Technical Manual
3 - 26 RAA016CEN
Analysis cycle technology
4 - Analysis cycle technology
1. Analysis cycle description ....................................................................................2

1.1. Pentra 60 NON equipped with Takasago valves .................................................................. 2
1.2. Pentra 60 equipped with Takasago valves (# 33 & 34) ......................................................... 5
2. Measuring principles ............................................................................................8

2.1. Multi Distribution Sampling System (MDSS) ........................................................................ 8
2.2. RBC/PLT ............................................................................................................................... 9
2.3. HGB Measurement ............................................................................................................. 11
2.4. HCT measurement .............................................................................................................. 12
2.5. RDW calculation ................................................................................................................. 12
2.6. MCV, MCH, MCHC calculation .......................................................................................... 12
2.7. MPV Measurement ............................................................................................................. 12
2.8. PCT Calculation .................................................................................................................. 13
2.9. PDW calculation ................................................................................................................. 13
2.10. WBC and differential count .............................................................................................. 13
3. Raw counts ..........................................................................................................17

3.1. Selection of the raw counts ................................................................................................ 17
3.2. Description of the raw counts ............................................................................................ 17
Technical Manual
RAA016CEN 4-1
Analysis cycle description
1. Analysis cycle description
1.1. Pentra 60 NON equipped with Takasago valves
■ Aspiration of blood sample from the tube

(53µl)
■ Translation of sampling carriage over Rinse
chamber.
■ External rinsing of the needle and reject of
3µl of blood (not used).
■ Translation of sampling carriage over DIL1

chamber (first dilution), descent of the
needle.
■ Positioning the needle point into a
tangential flux of ABX Diluent (1.65ml) and
synchronised distribution of 10µl of blood.
■ Bubbling
■ Translation of sampling carriage over WBC/

BASO count chamber.
tangential flux of 2ml of ABX Basolyse 2 and
■ Bubbling
Dilution:
Blood Volume: 10 µl
ABX Basolyse 2 Volume: 2000 µl
Dilution Rate: 1/200
Technical Manual
4-2 RAA016CEN
■ Translation of sampling carriage over LMNE

mixing chamber.
tangential flux of 1ml of ABX Eosinofix and
■ Bubbling
Dilution:
ABX Eosinofix Volume: 1000 µl
ABX Diluent Volume: 1000 µl
Final Dilution Rate: 1/80

chamber
■ Distribution of the 5µl of residual blood
during the needle internal and external
rinsing.
■ WBC and LMNE counting:

- 1ml of ABX Diluent into LMNE chamber
- Dilution transfer trough LMNE flowcell and
counting during 12 seconds
- WBC\BASO counting
■ Translationof sampling carriage over Dil1

chamber.
■ Sample of 42.5 µl of the first dilution.
Technical Manual
RAA016CEN 4-3

chamber.
■ Rinsing of exterior of sampling needle with
0.4 ml of ABX Diluent.
■ Translation of sampling carriage over RBC

and PLT count chamber.
■ Distribution of the 42.5µl of first dilution into
a flux of 2.20ml of diluent.
■ Distribution of 0.3ml of diluent from the
interior of the needle.
Dilution:
Initial Blood Volume: 10 µl
Reagent Volume (RBC chamber): 2500 µl
HGB:
■ Distribution of 0.4 ml of ABX Diluent then
distribution of 0.36 ml of ABX Lyse into DIL1
chamber while bubbling.
■ WBC\BASO chamber rinsing: Draining and
filling with 1ml of ABX Cleaner and 1.5ml of
ABX Diluent.
■ RBC\PLT counting and HGB measurement.
■ Chambers cleaning and HGB blank:

- Draining and filling of DIL1 chamber with
2.7 ml of ABX Diluent
- Measurement of HGB blank
- Flowcell rinsing (about 1.6 ml of ABX
Diluent)
- Draining and filling of RBC chamber with
Technical Manual
4-4 RAA016CEN
1.2. Pentra 60 equipped with Takasago valves (# 33 & 34)
■ Aspiration of blood sample from the tube

(53µl)
chamber.
■ External rinsing of the needle and reject of
3µl of blood (not used).
■ Translation of sampling carriage over DIL1

chamber (first dilution), descent of the
needle.
tangential flux of ABX Diluent (1.65ml) and
■ Bubbling
■ Translation of sampling carriage over WBC/

BASO count chamber.
tangential flux of 2ml of ABX Basolyse 2 and
■ Bubbling
Dilution:
ABX Basolyse 2 Volume: 2000 µl
Dilution Rate: 1/200
Technical Manual
RAA016CEN 4-5
■ Translation of sampling carriage over LMNE

mixing chamber.
tangential flux of 1ml of ABX Eosinofix and
■ Bubbling
Dilution:
ABX Eosinofix Volume: 1000 µl
ABX Diluent Volume: 1000 µl

chamber
■ Distribution of the 5µl of residual blood
during the needle internal and external
rinsing.
■ WBC and LMNE counting:

- 1ml of ABX Diluent into LMNE chamber
- Dilution transfer trough LMNE flowcell and
counting during 12 seconds
- WBC\BASO counting
■ EV 33 and 34 commute (ON).

■ Aspirationof a part of the first dilution
through EV33 & 34 by the mean of the
Diluent reagent syringe (the lenght beetwen
EV33 and EV34 gives the exact volume for
the RBC sample).
Technical Manual
4-6 RAA016CEN
■ EV 33 and 34 commute (OFF).

■ The sample (42 µL) is pushed into RBC
chamber with a flux of 2.2 mL of ABX
Diluent.
■ Bubbling.
Dilution
Initial Blood Volume: 10 µL
Reagent Volume (RBC chamber): 2500 µL
HGB:
■ Distribution of 0.4 ml of ABX Diluent then
distribution of 0.36 ml of ABX Lyse into DIL1
chamber while bubbling.
■ WBC\BASO chamber rinsing: Draining and
filling with 1ml of ABX Cleaner and 1.5ml of
ABX Diluent.
■ RBC\PLT counting and HGB measurement.
■ Chambers cleaning and HGB blank:

- Draining and filling of DIL1 chamber with
- Measurement of HGB blank
- Flowcell rinsing (about 1.6 ml of ABX
Diluent)
- Draining and filling of RBC chamber with
Technical Manual
RAA016CEN 4-7
Measuring principles
2. Measuring principles
2.1. Multi Distribution Sampling System (MDSS)
2.1.1. CBC mode
diluent
In CBC mode, 30µl of whole blood is
Air
aspirated then delivered with reagents into
chambers as follows: 7 µl discarded
■ one specimen for the first

RBC/PLT dilution
and the HGB measurement. 10 µl WBC/BASO
■ another specimen for the BASO/WBC
count
10 µl RBC/PLT/HGB
3 µl discarded
2.1.2. Diff Mode
Diluent
In DIFF mode, 53µl of whole blood is

Air
aspirated, then delivered with reagents into
chambers as follows: 5 µl discarded
■ one specimen for the firstRBC/PLT dilution

and the HGB measurement. 25 µl Differential
■ another specimen for the BASO/WBC
count.
■ the last specimen for the LMNE matrix.
10 µl WBC/BASO
10 µl RBC/PLT/HGB
3 µl discarded
Technical Manual
4-8 RAA016CEN
2.1.3. Specimen distribution
Specimen distribution in the chambers is

carried out in a tangential flow of reagent
which allows perfect mixing of the dilution
and avoids any viscosity problems (this
multi distribution in a reagent flow is a
HORIBA MEDICAL patent).
2.2. RBC/PLT
2.2.1. detection principles
■ Measurement of impedance variation

generated by the passage of cells through a
calibrated micro aperture.
■ The specimen is diluted in an electrolytic
diluent (current conductor) and pulled
through the calibrated micro-aperture. Two
electrodes are placed on either side of the
aperture. Electric current passes through
the electrodes continuously.
■ When the cell passes through the aperture,
electric resistance between the two
electrodes increases proportionately with
the cell volume.
Technical Manual
RAA016CEN 4-9
■ The generated impulses have a very low

voltage, which the amplification circuit
increases, so that the electronic system can
analyze them and eliminate the background
noise.
■ Results:
Number of cells counted per volume unit x
calibration coefficient
Histograms
■ RBC:Distribution curves on 256 counting

channels from 30fl to 300fl.
■ PLT:Distribution curves on 256 channels

from 2fl to a mobile threshold. This
threshold moves according to the microcyte
population present in the analysis area.
Technical Manual
4 - 10 RAA016CEN
2.2.2. Technical characteristics of RBC and PLT counts

■ Method: impedance
■ Aperture diameter: 50 µm
■ Temperature of reaction: 35°C
■ Count vacuum: 200 mb
■ Count period: 2 x 5s
■ Initial blood volume: 10 µl
■ Vol. ABX Diluent: 2500 µl
■ Final dilution rate: 1/10000 (two successive dilutions are carried out)
■ Primary dilution for RBC and PLT:

- Blood 10 µl
- Vol ABX Diluent: 1700 µl
- Dilution: 1/170
■ Secondary dilution for RBC and PLT :
- Dilution: 42,5 µl (from the primary dilution)
- Vol ABX Diluent: 2500 µl
- Dilution: 1/58.8
■ Final dilution: 1/170 x 1/58.8 = 1/10000
2.3. HGB Measurement
2.3.1. Principles:
■ Alphalyse:
This reagent breaks down the RBC cell membrane and releases the Hemoglobin within the cell. The
hemoglobin, released by the lysing reagent, combines with the Potassium cyanide from the lysing
reagent to form a chromogenous cyanmethemoglobin compound. This compound is then measured
through the optical part of the first dilution chamber by way of spectrophotometry at a wavelength of
550nm.
■ Lysebio:
Reagent for erythrocyte lysis and cyanide-free determination of hemoglobin. By addition of agent of
lysis, hemoglobin is released. All the heme iron is oxidized and stabilized. Oxidation resulting
complexes are quantified by spectrophotometry at a wave length of 550nm.
2.3.2. Technical charasteristics of HGB measurement

■ Method: Photometry
■ Wavelength: 550 nm
■ Blood volume: 10 µl
■ Volume ABX Diluent: 1700 µl
■ Volume ABX Lyse: 400 µl
■ Complement ABX Diluent: 400 µl
■ Final dilution rate: 1/250
Technical Manual
RAA016CEN 4 - 11
2.3.3. Result
Final HGB result represents: Absorbance value obtained x coefficient of calibration.
2.4. HCT measurement
■ The height of the impulse generated by the passage of a cell through the micro-aperture is directly
proportional to the volume of the analyzed RBC.
■ The hematocrit is measured as a function of the numeric integration of the MCV.
2.5. RDW calculation
■ The study of the RBC distribution detects erythrocyte anomalies linked to anisocytosis.
■A Red Cell Distribution Width (RDW) will enable you to follow the evolution of the width of the curve in
relation to the cell number and average volume.
■ RDW = (K X SD) / MCV
■ With:
- K = system constant
- SD = Determined standard deviation according to statistical studies on cell distribution.
- MCV = Mean Corpuscular Volume of erythrocytes
2.6. MCV, MCH, MCHC calculation
■ MCV (Mean Cell Volume) is calculated directly from the RBC histogram.
■ MCH (Mean Corpuscular Hemoglobin) is calculated from the Hgb value and the RBC number.
The mean hemoglobin weight in each RBC is given by the formula:
MCH (pg) = HGB/RBC x 10
■ MCHC (Mean Corpuscular Hemoglobin Concentration) is calculated according to the HGB and HCT
values. Mean HGB concentration in the total volume of RBC is given by the formula:
MCHC (g/dL) = HGB/HCT x 100
2.7. MPV Measurement
The MPV (Mean Platelet Volume) is directly derived from the analysis of the platelet distribution curve.
Technical Manual
4 - 12 RAA016CEN
2.8. PCT Calculation
Thrombocrit is calculated according to the formula:
PCT% = PLT (103/µl) x MPV (µm3) / 10 000
2.9. PDW calculation
■ PDW (Platelet Distribution Width) is

calculated from the PlT histogram.
■ The PDW is represented by the width of the

curve between 15% of the number of
platelets starting from 2 fl (S1), and 15% of
the number of platelets beginning with the
variable top threshold (S2).
2.10. WBC and differential count
2.10.1. General principles

The WBC count is carried out twice by two different sensors:
■ In the BASO count chamber at the same time as the BASO count,
■ Inthe optical chamber during the acquisition of the LMNE matrix.
The reference count is the one obtained in the WBC and BASO count chamber.
2.10.2. BASO / WBC Count
■ Detection principle is the same as for RBC.

Differentiation betwen BASO and other
leukocytes is obtained by means of the ABX
Basolyse 2 specific lysing action.
■ All
the WBC are counted between the
electrical threshold <0> threshold <BA3>.
The basophils are located from threshold
<BA2> to threshold <BA3>.
■ Results
- WBC: Number of cells per volume x coefficient of calibration.
- BASO: Number of cells per volume x coefficient of calibration in percentage regarding the total
number of leukocytes (BASO + WBC nuclei)
Technical Manual
RAA016CEN 4 - 13
2.10.3. Technical characteristics of WBC/BASO counts

■ Method: impedance
■ Count vacuum: 200 mb
■ Count period: 2 x 6 s
■ Initial blood volume: 10 µl (CBC or CBC/DIFF)
■ Vol. ABX Basolyse 2: 2000 µl
2.10.4. LMNE matrix
■ TheWBC and Differential count are based

on 3 essential principles:
- The double hydrodynamic sleeving

«DHSS» (HORIBA MEDICAL patent).
- The volume measurement (impedance

changes).
- The measurement of transmitted light with

0° angle, which permits a response
according to the internal structure of each
element and its absorbance by means of
incident light diffusion.
■ 25µl of whole blood is delivered to the

LMNE chamber in a flow of ABX Eosinofix.
This reagent lyses the RBC, stabilizes the
WBC in their native forms and stains the
eosinophil nuclei with a specific coloration.
■ The solution is then stabilized with ABX

Diluent and transferred to the measuring
chamber. Each cell is measured both in
absorbance (cytochemistry) and resistivity
(volume).
2.10.5. Technical characteristics of WBC counts during the acquisition of

the matrix
■ Method: impedance with hydrofocus
■ Flow diameter: 42 µm
■ Injection duration: 12 s
■ Injected volume: 72 µl
Technical Manual
4 - 14 RAA016CEN
■ Incubation duration: 12 s
■ Initial blood volume: 25 µl (CBC or CBC/DIFF)
■ Vol. ABX Eosinofix: 1000 µl
■ Vol. ABX Diluent: 1000 µl
■ No cell in the flowcell

Baseline
■ Poorly stained (agranular) cell in the flowcell

Low absorbance
■ Hyper segmented with complex granulary

and staining High absorbance
■ Results:
From these measurements, a matrix is drawn up with volumes on the X-axis and optical transmission
on the Y-axis. The study of the matrix image allows the clear differentiation of 4 out of 5 leukocyte
populations. As a matter of fact, the basophil population is very small compared to the others.
■ LYMPHOCYTES: The lymphocyte is a very small round shaped cell with condensed cytoplasm and
large nucleus. This cell is normally positioned in the lower part of the Y-axis, as well as the lower part
of the X-axis because of its small size. The far left side of the lymphocyte area (LL) should normally be
empty. Any detection of cells in the LL area indicates Small lymphocytes, Platelet aggregates, NRBCs
Technical Manual
RAA016CEN 4 - 15
(Nucleated Red Blood Cells), and/or improperly adjusted flowcell alignment. Background noise may
also be detected in this area if the interference is important.
■ MONOCYTES: The monocyte is a very large irregular shaped cell with large convoluted nuclei. The
nucleus contains folds and sometimes vacuoles. The cytoplasm is also large with non-granular
intracellular material. This cell does not scatter or absorbs a large amount of light when passing
through the flowcell. It is positioned in the lower part of the Y-axis. Because the monocyte is a large
cell, it is positionned on the right side of the X-axis.
■ NEUTROPHILS: The neutrophil is larger in size than the lymphocyte. It contains granular material in its
cytoplasm along with a segmented nucleus. Due to these cellular features, more light will pass through
neutrophils in the flowcell. As a result, neutrophils go higher on the Y-axis and spread along the X-axis
according to their maturity. Hyper-segmentation and increased granules place this population higher
along the Y-axis.
■ EOSINOPHILS: The eosinophil is somewhat like the neutrophil. It contains granular material and
segmented nuclei within the cytoplasm. The granular material is colorized with a reagent before
passing through the light beam in the flowcell. Due to the colorization action of the reagent, the
eosinophils are placed in the highest part of the Y-axis. Hyper-segmentation and increased granules
place this population in the top right area of the matrix.
■ Additional parameters: LIC (Large Immature Cells) and ALY (Atypical Lymphocytes) complete the panel
available on the matrix.
Immature granulocytic cells are detected by their larger volumes and by the increased granules, which
allow more light to pass through the cells and increase the intensity of scattered light. Therefore, cells
such as metamyelocytes will be found to the right of the neutrophils and almost at the same level.
Myelocytes and promyelocytes will be found on the far right of the matrix in the saturation position. The
metamyelocytes, myelocytes, and promyelocytes will all be classed as (LIC) and there given results will
be included in the neutrophil value. The Blast cells will be generally located to the right of the monocyte
population and, as such, will increase the (LIC) count. Small blast cells will be found between the
normal lymphocyte and mococyte populations (ALY).
Platelet aggregates and debris from RBC cell fragments are found in the background noise area, at the
lower left corner of the matrix. Most of the cell population thresholds are fixed and give the normal
limits for the normal leukocyte morphologies. Changes in the morphology of a specific population will
be indicated on the matrix by a shift in the corresponding population.
A Blast alarm is generated from increased counts within the (LIC) area; this is correlated with the Blast
detection on the Basophil histogram.
Large lymphocytes are usually detected in the (ALY) area, where reactive lymphoid forms, stimulated
lymphocytes, and plasmocytes are also found as well.
Technical Manual
4 - 16 RAA016CEN
Raw counts
3. Raw counts
3.1. Selection of the raw counts
The raw counts are available on printout only.

Enter: Setup / Printer / Printer configuration and select «raw values».
3.2. Description of the raw counts
The raw counts are printed at the bottom of the Report printout.
■ WBC1 / WBC2: Number of WBCs counted in WBC/BASO chamber during the first and the second
period of count.
■ RBC1 / RBC2: Number of RBCs counted in RBC/PLT chamber during the first and the second period
of count.
■ HCT1 / HCT2: First & second HCT measurements.
■ PLT1 / PLT2: Number of PLTs counted in RBC/PLT chamber during the first and the second period of
count.
■ HGBB: Measurement of the HGB blank.
■ HGBM: HGB measurement.
■ LYM / MON / NEU / EOS: Number of cells located in their respective population areas.
■ NO / LL / RM / LN / FLN / FMN / FNE / RN : Number of cells located in their respective flag areas.
■ ALY / C127: Number of cells located in their respective areas.
■ LIC: Number of cell located on RN + RM + C127
Technical Manual
RAA016CEN 4 - 17
Raw counts
C127
EOS
NO
FNE
RN
LN NEU
N
FM
FLN
MON RM
LL LYM
ALY
■ WBC: WBCs used for for the LMNE and BASO balance adjustments.
■ RA#: Number of cells counted
■ DIFF #: number of impact in LYM + MON + NEU + EOS population areas
■ CO%: Correlation percentage: % of cells passing through the flowcell in the correct time (transfer time,
between the aperture and the optical measurement).
Technical Manual
4 - 18 RAA016CEN
Software
5 - Software
1. Versions .................................................................................................................2
2. USER menu overview ...........................................................................................3
3. MAINTENANCE menu overview ...........................................................................4
4. SETUP menu overview .........................................................................................5
5. TECHNICIAN menu overview ...............................................................................6
Technical Manual
RAA016CEN 5-1
Software
Versions
1. Versions
■ Instruments NON equipped with TAKASAGO valves: V2.3.0

■ Instruments equipped with TAKASAGO valves: V2.3.2
Technical Manual
5-2 RAA016CEN
Software
USER menu overview
2. USER menu overview
1 - START CYCLE
2 - CONTROL MODE 1 - AUTOCAL.
2 - CHANGE COEFF.
3 - PRINT COEFF.
3 - CALIBRATION 4 - REPEATIBILITY
4 - REAGENTS 1 - LEVEL / CHANGE
2 - DAILY WORKLOAD
3 - PRIME
5 - MAINTENANCE See MAINTENANCE menu overview
6 - SETUP See SETUP menu overview
Technical Manual
RAA016CEN 5-3
Software
MAINTENANCE menu overview
3. MAINTENANCE menu overview
MAINTENANCE
1 - AUTOCONTROL
1 - BACK FLUSH
2 - CLEANING
1 - CHAMBERS
2 - RINSE 2 - CYTOMETER
3 - DRAIN CHAMBERS 1 - RINSE

2 - FIRST DILUTION
3 - LMNE
4 - WBC/BASO
3 - HYDRAULIC SYS. 4 - CONC. CLEANING 5 - RBC/PLT
6 - ALL CHAMBERS
7 - DILUENT RESERVOIR
1 - SAMPLING NEEDLE
2 - CARRIAGE
3 - SAMPLING SYR.
4 - MECHANICAL SYS. 1 - INITIALIZATION 4 - DRAINING SYR.
5 - COUNTING SYR.
6 - CYTOMETER SYR.
2 - CHECK MOTORS 7 - DILUTION SYR.
3 - CHECK VALVES 1 - 1 to 11
2 - 12 to 16
3 - 17 to 19
5 - TECHNICIAN 4 - 20 to 26
4 - MAINTENANCE 5 - 27 to 31
CARRIAGE POS.
See TECHNICIAN menu overview
Technical Manual
5-4 RAA016CEN
Software
SETUP menu overview
4. SETUP menu overview
1 -CBC
6 - SETUP
1 - NORMAL RANGES 2 - DIFF
2 - PANIC RANGES 1 -CBC

1 - DATE FORMAT 2 - DIFF
1 - DATE / TIME 2 - SET DATE/TIME
3 - FLAG SENSITIVITY
4 - THRESHOLDS
2 - UNITS
1 - RS232 CONFIG.
1 - NUMERICAL RESULT
2 - SENDING CONFIG. 2 - FLAGS & PATHOLOGIES
3 - HISTOGRAMS & THRESHOLDS
4 - PATIENT FILE
3 - LAB.LIMITS 5 - RAW DATA
3 - ABX FORMAT
4 - SEND LATEST RES.
1 - CALIBRATION
4 - RS232
2 - IDENTIFICATION MODE
3 - AUTOCLEAN FREQUENCY
3 - PRINTER CONFIG.
5 - PRINTER
4 - CHANGE PASSWORD
4 - PRINT LATEST RES.
5 - LANGUAGES
6 - OTHERS 6 - REAGENT CAPACITIES
7 - CYCLES #
8 - CONFIGURATION
9 - WBC BALANCE
Technical Manual
RAA016CEN 5-5
Software
TECHNICIAN menu overview
5. TECHNICIAN menu overview
MAINTENANCE 1 - DILUTIONS 1 - HB BLANK ADJUSTMENT

2 - APPERTURE CURRENT
3 - RBC/PLT GAIN
4 - WBC/BASO GAIN
5 - LMNE ADJUSTMENT
2 - MEASUREMENT
1 - ADJUSTMENT
2 - REFERENCE
1 - AUTOCONTROL
1 - REAGENT HEATING SYSTEM
3 - HEATING SYSTEMS 2 - AMBIANCE BOX
2 - CLEANING
1 - ADJUSTMENT
2 - REFERENCE
3 - HYDRAULIC SYS.
4 - BUBBLING
4 - MECHANICAL SYS.
1 - DRAINING
5 - TECHNICIAN 5 - SENSOR CHECK 2 - LMNE TRANSFER
1 - COUNTING
6 - VACUUM CHECK 2 - DRAINING
7 - BURN-IN
8 - USER MODE
1 - CYCLE #
2 - MAINTENANCE CARRIAGE POSITION
9 - OTHERS 3 - PARK SYRINGES POSITION
4 - SPECIAL VALUES
Technical Manual
5-6 RAA016CEN
Alarm & error list
6 - Alarm & error list
1. Printer .....................................................................................................................2
2. Transmission .........................................................................................................2
3. Calibration .............................................................................................................2
4. Technical Operations ............................................................................................3
5. Reagents ................................................................................................................3
6. Temperature ..........................................................................................................3
Technical Manual
RAA016CEN 6-1
Alarm & error list
Printer
1. Printer
Displayed message Causes Action

The printer is disconnected, - Switch on or
switched off or has not been Printout operations disabled - press "ON LINE"or
selected - see the printer user’s manual
Defect on printer, make sure there - Feed paper or
Printout operations disabled
is paper - see the printer user’s manual
2. Transmission

No ENQ Character Received On
RS232
No ACK Character Received On
RS232
Read Error On RS232 Check the RS 232 configuration
Defect on transmission
Menu "SETUP / RS 232 / RS232
Write Error On RS232 operations
CONFIGURATION"
Timeout overflow on RS232
Result transmission impossible
Transmission mode &/or format
incompatible
3. Calibration

Perform an autoconcentrated
Coefficients out of the ranges
Coefficients outside the limits cleaning
given section 3
Run another calibrator vial
Data not saved, value out of incoherent value entered by the
Re-type in the item
range operator
Incoherent date entered by the
Illegal date Re-type in the date
operator
Minimum tagged CBC incorrect, Selected results for calibration
Select at least 3 results
at least 3 calculation < 3
Technical Manual
6-2 RAA016CEN
Alarm & error list
Technical Operations
4. Technical Operations

Emergency stop, Run an Blocked motor, Incorrect Drains, control the motor operations :
autocontrol Thermal door opened Menu
"MAINTENANCE / MECHANICAL
.....not reaching home Blocked motor SYSTEMS / CHECK MOTORS"
close the door and rerun the
Thermal door opened open during a cycle
cycle
Incoherent time entered by the
Illegal time Enter the correct time
operator
5. Reagents

replace the diluent container
No diluent, check level diluent reservoir empty (menu "REAGENTS/LEVEL -
CHANGE")
Replace the bottle (menu
Reagent low level (reagent name) none
"REAGENTS/LEVEL-CHANGE")
Message triggered at the end of control the reagent levels or
Reagent low level
the Startup /and replace it.
Call your HORIBA Medical
Drain time out Draining problems representative Service
Department
6. Temperature

Call your HORIBA Medical
Temperature out of range Thermic regulation problem representative Service
Department
Operating temperature not
Heating coil initialization Wait for a few minutes
reached
Technical Manual
RAA016CEN 6-3
Alarm & error list
Temperature
Technical Manual
6-4 RAA016CEN
Maintenance
7 - Maintenance
1. Maintenance ....................................................................................................... 7-4
2. Procedures ......................................................................................................... 7-7
1. Installation kit XEA791D .......................................................................... RAS235C-2
2. Installation ............................................................................................... RAS235C-3

1. 6 month maintenance kit ......................................................................... RAS236C-2
2. Rinsing block ........................................................................................... RAS236C-3
3. Reagent syringe ...................................................................................... RAS236C-4
4. Draining and counting syringes ............................................................... RAS236C-6
5. Axis lubricating ........................................................................................ RAS236C-8
6. Instrument cleaning ................................................................................. RAS236C-9
7. Check up after intervention ..................................................................... RAS236C-9

1. Yearly maintenance kit XEA486BS .......................................................... RAS237C-2
2. Sampling syringe O’ring replacement ..................................................... RAS237C-3
3. 5 Diff syringe O’ring replacement ............................................................ RAS237C-4
4. Optical bench lamp replacement ............................................................ RAS237C-5
5. LMNE flowcell ......................................................................................... RAS237C-6
6. Diluent reservoir joint replacement .......................................................... RAS237C-8
7. Draining chamber O’ring replacement .................................................... RAS237C-9
8. Counting heads ..................................................................................... RAS237C-10
9. Additional maintenance ......................................................................... RAS237C-12
10. Check up after intervention ................................................................. RAS237C-12

1. Kit XEA581BS .......................................................................................... RAS238C-2
2. Sampling parts ........................................................................................ RAS238C-3
3. Reagents syringe ..................................................................................... RAS238C-4
4. Draining & counting syringes ................................................................... RAS238C-6
5. Additional maintenance ........................................................................... RAS238C-8
6. Check up after intervention ..................................................................... RAS238C-8

1. Preparation .............................................................................................. RAS239C-2
2. Manual decontamination ......................................................................... RAS239C-2
3. Analysis circuit decontamination ............................................................. RAS239C-3
4. Drain & Rinse ........................................................................................... RAS239C-3

1. LMNE adjustment parameters ................................................................ RAS240C-2
2. Requirements .......................................................................................... RAS240C-4
3. LMNE Flowcell adjustment check. .......................................................... RAS240C-5
4. LMNE Flowcell position adjustment ........................................................ RAS240C-6
5. Final adjustment ...................................................................................... RAS240C-8
6. Transfer time adjustment ........................................................................ RAS240C-9
Technical Manual
RAA016CEN 7-1
Maintenance
1. Alignment check ...................................................................................... RAS241C-2

2. Alignment adjustment ............................................................................. RAS241C-3

1. Introduction ............................................................................................. RAS242C-2
2. Needle home check & adjustment .......................................................... RAS242C-3
3. All carriage positions check & adjustment .............................................. RAS242C-4

1. Main board general view ......................................................................... RAS243C-2
2. Power supply check ................................................................................ RAS243C-3
3. Motor current adjustment ........................................................................ RAS243C-4
4. Threshold adjustment .............................................................................. RAS243C-5
5. Draining sensor adjustment .................................................................... RAS243C-6
6. Transfer sensor adjustment ..................................................................... RAS243C-7
7. HGB blank adjustment ............................................................................ RAS243C-8
8. RBC\PLT gain adjustment ....................................................................... RAS243C-9
9. WBC\BASO gain adjustment ................................................................ RAS243C-10

1. Reagent heating coil ................................................................................ RAS244C-2
2. Thermostatic compartment ..................................................................... RAS244C-5

1. Draining syringe vacuum check .............................................................. RAS245C-2
2. Counting syringe vacuum check ............................................................. RAS245C-3
3. Counting syringe vacuum adjustment ..................................................... RAS245C-4

1. Dismantling .............................................................................................. RAS247C-2
2. Mounting ................................................................................................. RAS247C-4

1. Heater assy dismantling .......................................................................... RAS248C-2
2. Heater assy replacement ........................................................................ RAS248C-5

1. Bubbling check and adjustment ............................................................. RAS249C-2
2. Bubbling values ....................................................................................... RAS249C-2

1. Dismantling & Replacement .................................................................... RAS250C-2
2. Power supply check ................................................................................ RAS250C-4

2. Start Cycle Microswitch Dismantling ...................................................... RAS251C-2
3. Start Cycle Microswitch replacement ..................................................... RAS251C-3

1. Preliminary ............................................................................................... RAS252C-2
2. Repeatability ............................................................................................ RAS252C-2
3. Calibration ............................................................................................... RAS252C-3
Technical Manual
7-2 RAA016CEN
Maintenance

2. Start cycle condition ............................................................................... RAS253C-2
3. Chambers ................................................................................................ RAS253C-2
4. Step by step analysis cycle ..................................................................... RAS253C-3

1. Dismantling & Replacement .................................................................... RAS254C-2
2. Control ..................................................................................................... RAS254C-4
3. Lamp replacement .................................................................................. RAS254C-5

1. Barcode reader configuration check ....................................................... RAS255C-2
2. Barcode reader setup .............................................................................. RAS255C-3

1. Calibration ............................................................................................... RAS304C-2
2. Forced calibration ................................................................................... RAS304C-4

1. Purpose ................................................................................................... RAS680A-2
2. Chamber access ..................................................................................... RAS680A-2
3. Valve assembly replacement ................................................................... RAS680A-3
Technical Manual
RAA016CEN 7-3
Maintenance
Maintenance
1. Maintenance
■ Customer maintenance has to be carried out according to the recommended frequency

chart table and after having attended an HORIBA Medical approved customer training
course.
■ The system warranty may be affected if damage occurs after a non trained technician
intervention or if replaced spare parts and consumables do not come from an HORIBA
Medical approved origin.
1.1. Daily customer maintenance
■ No special adjustments or maintenance has to be done on your equipment if the recommended

startup and shutdown procedures are explicitly respected.
■ See the User Manual for the daily rinsing and cleaning of the system.
1.2. Weekly customer maintenance
■ An overall check for cleanliness of the system is recommended every week.

■ All traces of blood or reagent have to be wiped off as soon as possible using a piece of cloth and
distilled water.
Never use solvent or abrasive cleaning material to clean the system.
Technical Manual
7-4 RAA016CEN
Maintenance
Maintenance
1.3. Maintenance table
The Pentra 60 maintenance is scheduled on different maintenance kits. The maintenance schedule
depends on the number of cycles per day, follow the chart table to know the kit part number to use:
Cycle per day <30 30 to 120 >120 Part number

Maintenance each: 1Y 2Y 6M 1Y 18M 2Y 6M 1Y Kit to use
Sampling needle O’ring X X X X X X X X
XEA485AS
Reagent syringe O’ring X X X X X X X X
procedure RAS236
Drain. & count. syringes O’ring X X X X X X X X
Sampling syringe X X X X X X
5 DIFF syringe X X X X X X
Draining chamber X X X X X X
XEA486BS
Optical bench lamp X X X X X X
procedure RAS237
LMNE Flowcell coaxial X X X X X X
Diluent reservoir joint X X X X X X
Counting heads X X X X X X
Sampling needle replacement X X X
Reagent pistons replacement X X X XEA581BS
Drain. & count. syringes pistons X X X procedure RAS238
replacement
1.4. Maintenance kits
1.4.1. 6 month Maintenance kit

Follow the RAS236 procedure for maintenance with the XEA485AS kit.
XEA485AS
Part number Qty Designation
FAA053A 1 O’ring 1.4x1.25 Fluocarbon (sampling needle)
FAA065A 1 O’ring 6.30x2.40 Silicon ( Reagent syringe )
XDA621A 2 O’ring 30.80x3.60 + washer ( counting + draining syringe)
XDA622A 4 O’ring 15.54x2.62 + washer ( Reagent syringe )
XEA019A 1 Silicon grease
1.4.2. Yearly Maintenance kit

Follow the RAS237 procedure for maintenance with the XEA486BS kit.
XEA486BS
DAJ007A 1 Optical bench lamp
FAA040A 1 O’ring 12.1x2.7 5DIFF syringe
FAA046A 2 O’ring 2.75x1.6 Coaxial cable
FAA064A 2 O’ring 14.2x1.52 Sampling syringe
Technical Manual
RAA016CEN 7-5
Maintenance
Maintenance
XEA486BS
FAA066A 4 O’ring 13.1x1.6 Draining chambers
FAA067A 4 O’ring 2.4x1.9 5DIFF syringe
GBG275A 4 Counting head 0.5 joint
XEA663A 1 LMNE flowcell coaxial kit
XEA286AS 1 Waste chamber washer + joint
JAJ007A 1,5ML Polyeth. Tape
GBL0073 2 Jaws springs
KAL017A 4 Pin 3x12
FAA049A 2 O’ring 2.2x1 silic. 70 SH
1.4.3. Pistons Maintenance kit

Follow the RAS238 procedure for maintenance with the XEA581BS kit.
XEA581BS
GBC030A 4 Reagent syringe piston
GBC031A 1 Lyse reagent syringe piston
GBG091A Carriage,needle guide P60
XDA619BS 1 P60 OT equiped needle
GBG260A 2 Piston D=31 Spacer
GBG274A 2 Piston D=31
KAA010A 6 CHC M3x25 screws
Technical Manual
7-6 RAA016CEN
Maintenance
Procedures
2. Procedures
Maintenance and adjustments required for Pentra 60 are divided into procedures according
to the concerned assemblies. This should make any update easier as all interventions can be
done with the corresponding procedure on its own.
Each procedure has to be read entirely before starting intervention.
Procedures must be performed on a clean instrument.

If the instrument is suspected not to be perfectly clean, perform a concentrated cleaning.
Disposable gloves should be worn.
2.1. Required tools and products
Tools\Product Designation P/n

RBC/PLT Latex LAD002BS
Silicon grease LAM004A
Vactra oil 100 mL XEA821A
Distilled water
Ethanol or essence C
Liquid soap
Voltmeter MAM001AS
Thermometer MAM009AS
Barflex
Clamps
Cutting pliers
Dynamometric screwdriver A300 XEA585AS
Flat screwdriver
Cruciform screwdriver
Hexagonal keys
Torx keys
Gauge set MAJ004A
Pair of scisors
Scalpel
Felt pen
Syringe 5ml
Empty sample tubes
Micropipette tip
Soft tissue
Flat piece of stiff plastic
Technical Manual
RAA016CEN 7-7
Maintenance
Procedures
2.2. Procedure list
P/n and tittle Concerns

RAS235C: Installation Installation of Pentra 60
O’ring replacement (Rinsing block, Reagent syringe,
RAS236C: 6 month maintenance Draining & counting syringes)
Axis lubricating & Instrument cleaning
Sampling & 5 diff syringe O’ring replacement
Optical bench lamp replacement
LMNE flowcell coaxial replacement
LMNE flowcell adjustment check
Diluent reservoir joint replacement
Draining chambers O’ring replacement
Counting heads coaxial cable O’ring replacement
Counting heads aperture joint replacement
6 month maintenance
Check up after intervention
Sampling parts replacement
RAS238C: Every 2 years maintenance Reagent syringe pistons replacement
Draining & counting syringe pistons replacement
Instrument decontamination before maintenance
operations in the following cases:
- Instrument removed from biohazardous area
- Maintenance intervention on contaminated assemblies
RAS240C: LMNE flowcell adjustment LMNE Flowcell adjustment
RAS241C: Chambers adjustment Chambers position check and alignment
RAS242C: Needle adjustment Check and adjustment of the needle
RAS243C: Main board adjustment Main board adjustment
Heating coil and Thermostatic compartment temperature
check & adjustment.
RAS245C: Vacuum adjustment Counting and draining syringe vacuum check & adjustment.
RAS247C: Covers dismantling Covers dismantling & mounting
RAS248C: Heater assy replacement Replacement of the heater assy
RAS249C: Bubbling adjustment Bubbling adjustment.
RAS250C: Power supply replacement Power supply dismantling
RAS251C: Start cycle microswitch
Start cycle microswitch replacement
replacement
RAS252C: Check up after intervention Check up and control of the instrument accuracy
RAS253C: Step by step analysis cycle Analysis cycle description
RAS254C: Optical bench dismantling &
Dismantling, Replacement and Control of Optical Bench
replacement
RAS255C: External barcode reader
Barcode Reader test and configuration
configuration
RAS304C: LMNE balance adjustment LMNE Balance calibration and forced calibration.
RAS680A: Takasago valve assembly
Takasago valve assembly dismantling
dismantling
Technical Manual
7-8 RAA016CEN
RAS235C
Installation
■ Concerns
■ Installation of Pentra 60
■ Required tools
■ None
■ Required products
■ None
■ Intervention time
■1 hour
■ Frequency
■ On request
■ Specific kit or consumables

■ Installation kit: XEA791D
Disposable gloves, eyes protection and lab coat

must be worn by the operator.
Local or national regulations must be applied in all
the operations.
Installation
1. Installation kit XEA791D

DAJ007A 1 Lamp 20W 9,5V
DBH001A 3 Tyrap L=92
DBH002A 3 Tyrap L=140
DBK003A 1 Flat cable adhesive support
EAB021A 1 "Y" connector ø 3mm
EAB033A 1 «T» connector ø 1,6mm T410-6
EAB035A 1 «T» connector ø 2,3mm T220-6
EAC010A 2 Female luer connector I=3
EAC019A 1 Male luer connector I=3
EAE005A 2 ML Tygon tube ø 1,02mm (0,040’’)
EAE053A 2 ML Tygon tube 3,2x6,4 type 2075
EAE028A 2 ML Cristal tube 4x6
EAE034A 2 ML Tygon tube ø 2,54mm (0.100’’)
FAA053A 1 O’ring 1,4x1,25 Fluocarbon
FBH016A 1 Dust cover
FBL001A 2 Rubber cap 2 holes
GAK302A 3 Bottle cap
GBG282A 1 1/4 turn door key
GBG245A 3 Reagent straw stopper ø 28
GBG145A 1 Reagent straw stopper ø 20
GBG155A 1 Capsule ø 25
JAJ011A 1 Box 33x25x08
MAB002A 1 Hexagonal bent key 2,5mm
MAB018A 1 Hexagonal bent key 3mm
MAB090A 1 Torx bent key T10
XDB136A 1 3V liquid valve (without self)
XDA621A 1 O’ring 30,8x3,8 + Washer
XEA018A 1 Diluent straw Lg=360mm
XEA019A 1 Grease
XDB199A 1 PES60 Diluent tube
XDA693A 1 P60 Waste tube
MAB001A 1 Hexagonal bent key 2mm
HAW003A 1 Sticker, reagents names P60
Technical Manual
RAS235C - 2 RAA016CEN
Installation
2. Installation
2.1. Instrument installation
To operate the instrument correctly, install it in a place fulfilling the following conditions:
■ Place where the instrument and reagents are not thermally affected by direct sunlight and intense
radiant heat.
■ Place where it is not exposed to water or vapor.
■ Place where it is flat and free from vibration or shock.
■ Place where an independent power receptacle can be used.
■ Use a receptacle different from the one used by a device that easily generate noise such as a
centrifuge, etc...
■ Provide a space of at least 10 cm at the back of the instrument for arranging the power cable and
pipes.
■ Unpack the Instrument.

■ Open the pneumatic door on the right side
of the instrument.
■ Remove the 2 plastic parts blocking the
carriage for transportation:
Technical Manual
RAA016CEN RAS235C - 3
Installation
2.2. Reagent installation
■ Use «Luer» connectors, straws and

stoppers supplied with the installation kit to
connect integrated reagents, diluent and
wastes.
■ Bottles & Containers locations:

1 2 3 4
1 - ABX Cleaner
2 - ABX Basolyse 2
3 - ABX Eosinofix
4 - ABX Alphalyse or ABX Lysebio
5 - ABX Diluent
6 - Waste container. 80 cm maxi
■ Install the straw deep in the container.

■ Connect Diluent input tubing and waste
output tubing at the rear of the instrument.
6 5
Risk of erroneous results if diluent container is installed further than 80cm (31.5 in.) bellow
the instrument.
■ Diluent input tubing: cristal 3x6 / 2 meters (80 in.) maximum.
■ Waste output tubing: cristal 4x6 / 2 meters (80 in.) maximum.
Never pour reagent from one container to another. Particles present in the bottom of the old
container can contaminate the new reagent, causing unacceptable background counts,
especially for PLTs.
Technical Manual
Installation
2.3. Electrical connections
■ Check that the power supply module

voltage of the instrument corresponds to
the nominal voltage supply of the laboratory
and the country.
■ Connect the power supply cable.
Power supply
cable
2.4. Start Up
2.4.1. Start Up & Priming

■ Check that all is correctly connected.
■ Start the Analyzer.
■ Prime all the reagents: Enter in 4 - Reagents / 1 - Level change menu.
■ Use the «Up» and «Down» arrows to display the «CHANGE» key in reverse video.
■ Press «Enter» key: a prime cycle is automatically run.
■ The reagent level is updated to «100%».
■ Run a Startup by clicking the «Startup» button.
2.4.2. Check vacuum value

■ As the instrument accuracy depends on the altitude where the instrument is installed, check the
vacuum value. Follow «RAS245C: Vacuum adjustment» procedure and eventually adjust vacuum
value.
2.4.3. Check up after intervention

■ Follow «RAS252C: Check up after intervention» procedure to check instrument accuracy and
eventually re-calibrate it.
■ Before analyzing samples, visually inspect reagent lines and pumps for air bubbles. Repeat
priming if air bubbles are still present. Call the HORIBA Medical representative service
department if priming does not eliminate air bubbles.
■ Never initiate two Lyse prime cycles back-to-back. This causes excessive foaming in the
waste chamber. Run a blank cycle between each Lyse prime cycle.
Technical Manual
Installation
Technical Manual
RAS236C
6 month maintenance
■ Concerns
■ O’ring replacement:
- Rinsing block
- Reagent syringe
- Draining & counting syringes
■ Axis lubricating
■ Instrument cleaning
■ Required tools
■ Hexagonal keys
■ Dynamometric screw driver A302, A301, A300
■ Cutting pliers
■ Flat screw driver
■ Philips screw driver
■ Torx keys
■ Minoclair
■ Ethanol or essence C
■2 hours
■ Frequency
■ See Maintenance table

■6 month maintenance kit: XEA485AS
■ Oil:XEA821A
Disposal gloves, eyes protection and lab coat

the operations.
6 month maintenance
1. 6 month maintenance kit
XEA485AS
FAA053A 1 O’ring 1.4x1.25 Fluocarbon (sampling needle)
FAA065A 1 O’ring 6.30x2.40 Silicon ( Reagent syringe )
XDA621A 2 O’ring 30.80x3.60 + washer ( counting + draining syringe)
XDA622A 4 O’ring 15.54x2.62 + washer ( Reagent syringe )
XEA019A 1 Silicon grease
Technical Manual
6 month maintenance
2. Rinsing block
(1x FAA053A)
■ Switch on the instrument

■ Run Menu: Maintenance\Technician\Oth-
ers\Maintenance carriage position
■ Open the pneumatic access door (Right side
of the instrument)
■ Unscrew the 2 fixation screws (2x CHC
M3x25) of the Needle rinsing block on the
carriage.
■ Takeout the needle and the guide at the

same time (lift the needle locker to free the
needle).
■ Put the needle back to its support.
■ Open rinsing block assembly and replace

the old O’ring joint by the new one
(FAA053A) from the 6 month maintenance
kit (use one drop of silicon grease LAM004A
between 2 fingers for O’ring).
■ Reassemble following previous steps
backward.
■ When startup is done check that there is no
leaks.
Use a dynamometric screw driver to tighten the Rinsing block, tightening torques:
120 mN.m (17 Ozf.in)
Technical Manual
6 month maintenance
3. Reagent syringe
1 x FAA065A & 4 XDA622A
■ Switch off the instrument, disconnect

the power supply cable.
■ Open the left side panel and the
mother board door (lock it) to access
to the syringe.
■ Disconnect the following tubes:
- valve 6 inlet 3.
- valve 7 inlet 3.
- valve 8 inlet 3.
- valve 9 inlet 3.
- valve 11 inlet 3.
■ Unscrew the 2 fixation screws and
carefully remove the reagent syringe.
■ Push several times the piston over

the waste container to drain the
syringes. 1
■ On a piece of absorbant paper open
the reagents syringe (9x CHC M3x12
screws, 2x FX M3x12 screws).
2
■ Replace all O’rings and washers by
new ones from the 6 month
maintenance kit.
■ Use one drop silicon grease
(LAM004A) in between 2 fingers to
lubricate the O’rings. 8
■ Apply a thin film of grease 3
(LAM004A) on the GBC030A and
GBC031A pistons.
■ Reassemble in reverse order:
1_Reagent syringe body (GBG033A). 7
2_XDA622A x4.
3_Piston (GBC030A) x4.
4_FX M3x12 screws x2. 4
5_CHC M3x12 screws x9. 6
6_Reagent syringe bottom plate.
7_Lyse reagent piston (GBC031A).
8_O’ring (FAA065A). 5
Technical Manual
6 month maintenance
Use a dynamometric screw driver to tighten the syringe, tightenning torques:

- 9x CHC M3x12 : 400mN.m (56.8Ozf.in)
- 2x FX M3x12 : 400mN.m (56.8Ozf.in)
The thickness of the washer is matched to each O’ring thickness:

XDA622A = O’ring (FAA063A) + Washer (GBG149A).
For this reason, keep the O’ring and the washer together.
The lyse piston O’ring (small one) has no washer.
■ Remove any trace of oxydation on the

syringe holder with sand paper
■ Grease
abundantly the syringe holder
(LAM004A).
■ Cleanthe endless screw with ethanol or
essence C (Do not grease the endless
screw).
Technical Manual
6 month maintenance
4. Draining and counting syringes
4.1. O’ ring replacement on draining syringe
O’ring (FAA017A) + Washer (GBG148A) = XDA621A.
1 x XDA621A
■ Switchoff the instrument, disconnect the

power supply cable.
■ Open the right door to access to the syringe.
■ Disconnect the syringe electrical
connectors.
■ Unscrew the 4 CHC M4x16 syringe screws,
just few turns to release the silent blocks.
■ Put a piece of absorbant paper under the
syringe and disconnect all the tubes from the
syringe.
■ Remove the syringe.
■ Drain the syringe by pushing the piston nut up and down several times.
■ Open the syringe:
- 4x CHC M4x16 screws on old model.
- Rotation of the syringe body on new model.
Molded syringe (new model) Machined syringe (old model)
Open
Close
The chamber body of the new syringe is directly screwed on the motor block.
When the body is reinstalled on the motor block, it has to be rotated until the mark on the
body is located between the two marks of the motor block
Technical Manual
6 month maintenance
■ Replace the O’ring by the new one from the 6 month maintenance kit.
■ Use one drop silicon grease (LAM004A) in between 2 fingers to lubricate O’ring.
■ Apply a thin film of grease on the piston.
■ Make sure that the washer (GBG148A) is present on old model then reassemble in reverse order.
1_Motor assembly
2_O’ring (FAA017A)
3_Anti-extrusion ring (GBG219A)
4_Washer (GBG148A)
5_Syringe body
■ Clean the endless screw with ethanol or essence C (Do not grease the endless screw).
4.2. O’ ring replacement on counting syringe
1 x XDA621A
■ Switch off the instrument, disconnect the power supply cable.

■ Open the left side panel and the mother board door (lock it) to access to the syringe.
■ Follow the same procedure than for Draining syringe to replace the O’ring (See above).
Technical Manual
6 month maintenance
5. Axis lubricating
5.1. Needle carriage axis:
■ Clean the 2 axis with ethanol or essence C.

■ Apply a thin film of oil (XEA821A) on the
axis.
5.2. Sampling carriage axis:
■ Clean the 2 axis with ethanol or essence C.

■ Apply a thin film of oil (XEA821A) on the
axis.
Technical Manual
6 month maintenance
6. Instrument cleaning
■ Dilute the 12°cl bleach to 1 part of bleach for 4 of deionize water (1/5).
■ Instrument environment must be cleaned.
■ No sponge, nor cloth must be used. Only absorbant paper, thrown after use, in contamination bins,
have to be used. For small or sensitive assemblies use accurate drier papers.
■ All assemblies suspected to have been in contact with biohazardous material must be disinfected with
diluted bleach (the stainless steel must be bleached below 30°Celsius).
■ Blood stains or salt marks must be cleaned with spray detergent first.
■ Concerned assemblies:
- All the thermostated compartment
- Outer surfaces of the instrument (perpex, covers, reagent locations....)
- Waste connector plug
- Liquid valve push button
- Assemblies close to the needle
- Tube holder assembly
- Overflow trays
■ Reinstall all the assemblies and set up the instrument back to its initial configuration.
7. Check up after intervention
■ Before reassembling the covers, start the instrument and check that there is no leak.
■ Reassemble the covers then perform the «RAS252C: Check up after intervention» procedure to check
instrument accuracy and eventually re-calibrate it.
Technical Manual
6 month maintenance
Technical Manual
RAS237C
Yearly maintenance
■ Concerns
■ Sampling & 5 diff syringe O’ring replacement

■ Optical bench lamp replacement
■ LMNE flowcell coaxial replacement
■ LMNE flowcell adjustment check
■ Diluent reservoir joint replacement
■ Draining chambers O’ring replacement
■ Counting heads coaxial cable O’ring replacement
■ Counting heads aperture joint replacement
■ 6 month maintenance
■ Check up after intervention
■ Required tools
■ Hexagonal & tork keys
■ Dynamometric screwdrivers A300, A301, A302
■ Cutting pliers
■ Flat & cruciform screwdriver
■ Minoclair
■ Distilled water
■ RBC/PLT latex
■2 h
■ Frequency
■ See maintenance table

■ Yearlymaintenance kit XEA486BS
■6 month maintenance kit XEA485AS
Disposal gloves, eyes protection and lab coat

the operations.
Yearly maintenance
1. Yearly maintenance kit XEA486BS

DAJ007A 1 LAMP 20W 9,5V
FAA040A 1 O'RING, 5DIFF SYRINGE D=12,1
FAA046A 2 O'RING, COAXIAL CABLE
FAA064A 2 O'RING, SAMPL. SYRINGE P60/P80
FAA066A 4 O'RING, DRAINING CHAMBER P60/80
FAA067A 4 O'RING, 5DIFF SYRINGE D=2,4
GBG275A 4 O'RING, APERTURE D=0,5
XEA663A 1 CABLE, COAX LMNE FLOWCEL P60/80
XEA286AS 1 KIT, O'RING + WASHER
JAJ007A* 1,5 m ADHESIVE, TAPE FOR RACK EJECT.
GBL0073* 2 JAWS SPRING
KAL017A* 4 GOUPILLE CYLIND. 3 x 12
*: Not used on Pentra 60
Technical Manual
Yearly maintenance
2. Sampling syringe O’ring replacement
2 x FAA064A

ers\Maintenance carriage position
of the instrument)
■ Disconnect the tubes from the sampling sy-
ringe and unscrew the 2 fixation screws.
■ Openthe syringe by unscrewing the 3 FX FAA064A

M3x12 screws.
■ Replace both O’ring joints (use one drop of
silicon grease between 2 fingers to lubricate
O’rings).
■ Reassemble in reverse order.
Use a dynamometric screw driver to tighten the syringe:

Tightening torques: 400mN.m (56.8Ozf.in)
Technical Manual
Yearly maintenance
3. 5 Diff syringe O’ring replacement
1 x FAA040A + 4 x FAA067A
■ Switch off the instrument.
■ Open the left side panel and the mother
board door (lock it).
■ Gentlydisconnect the tubes from the 5DIFF
syringe and unscrew the 2 fixation screws.
FAA040A
- 2xFX M3x12
- 4xCHC M3x12 screws .
■ Replace all the O’rings (Use one drop of sil-
FAA067A
icon grease between 2 fingers to lubricate
O’rings).
■ Reassemble in reverse order.
Use a dynamometric screw driver to tighten the syringe:

Tightening torques: 400mN.m (56.8Ozf.in)
Technical Manual
Yearly maintenance
4. Optical bench lamp replacement
1 x DAJ007A
Please refer to "RAS254C, 3. Lamp replacement, page 5"
Technical Manual
Yearly maintenance
5. LMNE flowcell
5.1. coaxial replacement
1 x XEA663A
■ Open the cover.
■ Open the optical bench cover.
■ Disconnect the «T» connector (few diluent

drops will leak).
■ To access to the ground fitting under the

flowcell, unscrew the 2 fixation screws.
Technical Manual
Yearly maintenance
■ Slowly
turn the flowcell (take care to the
washers).
■ Unscrew the ground screw using a small
philips screwdriver.
■ Put the new LMNE coaxial instead.
■ Reassemble following previous steps back-
ward (except optical bench cover).
■ Enter in: Service\Hydraulics systems\Rinse menu and click the «Rinse cytometer» button to get rid
of the air bubbles stuck to the inner optical surfaces.
■ Check that there is no leak and that flowcell contains no or just a very few air bubbles.
■ Put back the optical bench cover.
5.2. Adjustment check
■ Run Menu: Maintenance\Techni-

cian\Measurement\LMNE Adjustment
■ Mix RBC/PLT Latex thoroughly.
■ Press ESC to start sampling
■ When optical bench is well adjusted:
■ Check that the displayed values are within the range in the following table.
Parameter Target Value Range

LMNE LAMP 6.00 5.50 to 6.50 V
TRANSFER TIME 200 150 to 250
RESISTIVE CHANNEL 53 51 to 55
ABSORBANCE CHANNEL 180 170 to 190
■ If you are out of range or to get a better adjustment follow the RAS240C: LMNE flowcell adjustment
procedure.
Technical Manual
Yearly maintenance
6. Diluent reservoir joint replacement
1 x XEA286AS
■ Switch on the instrument.
of the instrument).
■ RunMenu: Maintenance\Hydraulics sys-
tems\Drain chamber\Diluent reservoir
■ When cycle is finished disconnect the drain
tube under the reservoir.
■ Unscrew the 2 fixation screws under the di-
luent reservoir (few turns to release diluent
reservoir support).
■ Lift reservoir, take care that no tube is di-

connected from reservoir.
■ Unscrew the 4 reservoir screws on top.
■ Replace joint and washer by new one in the
yearly maintenance kit.
■ Locate new washer and joint against the
reservoir top, then the diluent reservoir
body.
■ Screw the 4 reservoir screws on top.
■ Put back diluent reservoir in its location and
block screws.
■ Connect drain tube under diluent reservoir.
■ When startup is done check that there is no
leak.
Use a dynamometric screw driver to tighten the diluent reservoir:

Tightening torques: 120 mN.m (17 Ozf.in)
Technical Manual
Yearly maintenance
7. Draining chamber O’ring replacement
■ Refer to RAS680A: Takasago valve assembly dismantling for chamber access.
4 x FAA066A
■ Make sure that the chambers are drained, if

not, run Menu: Maintenance\Hydraulics
systems\Drain chamber\All chambers to
drain the chambers.
■ Put a piece of absorbant paper or cloth un-
der the chambers assembly.
■ Gently turn the chamber’s bottom between 2
fingers.
■ Replace the O’ring.
■ Repeat for all the chambers the same oper-

ation.
Technical Manual
Yearly maintenance
8. Counting heads
8.1. Coaxial cable O’ring replacement
2 x FAA046A
■ Run Menu: Maintenance\Hydraulics sys-
tems\Drain chamber\All chambers to drain
the chambers.
■ Open the pneumatic access door (right side
of the instrument).
■ Put a piece of absorbant paper or cloth un-
der the chambers assembly.
■ Record the tube positions before disman-
tling the chambers assembly.
■ Disconnect chamber’s tubes except for the
wastes.
■ Unscrew the 3 screws from the chambers
assembly.
■ Dismantle the electrode by loosening the 2

fixation screws.
Technical Manual
Yearly maintenance
■ Use a previously cut micropipette tip to re-

place the electrode O’ring. 2
■ Reassemble the electrode then repeat the
same operation for the other electrode.
Use a dynamometric screw driver to tighten the counting heads:

Tightening torques: 120 mN.m (17 Ozf.in)
8.2. Aperture joint replacement
4 x GBG275A
■ Carefully dismantle the counting head by un-

screwing the 2 CHC screws.
■ Remove and plunge the aperture in distilled

water.
■ Clean the chamber and the counting head
with liquid soap.
■ Rinse thoroughly with distilled water.
■ Drythe exterior of the chamber with a soft
paper.
■ Replace the joints by new ones.
Technical Manual
Yearly maintenance
■ Do not introduce any sharp instrument inside so as to avoid damaging the inside of the
chamber and the aperture.
■ Do not manipulate the aperture using hard instruments. Clean the aperture with a piece of
soft paper or preferably in between 2 fingers.
■ It is recommended to reconnect the tubes on the counting head before reassembling the
electrode and the chamber in order to avoid applying constraint on the chamber.
■ Do not apply too much pressure on the electrode fixation screws as it can break the
aperture.
■ Tightening torque = 400 m.Nm (56.8 Ozf.in).
■ Reassemble the electrode and repeat the Joints

same operation with the other counting
head.
■ Reassemble in reverse order. Gently push
down the chamber assembly when tighten-
ning screws in order to keep the correct po- Aperture
sition.
9. Additional maintenance
■ The yearly maintenance includes the 6 month maintenance procedure.

■ Therefore, perform the 6 month maintenance procedure (see RAS236C: 6 month maintenance).
■ Reassemble the covers then perform the «RAS252C: Check up after intervention» procedure
Technical Manual
RAS238C
Every 2 years maintenance
■ Concerns
■ Sampling parts replacement

■ Reagent syringe pistons replacement
■ Draining & counting syringe pistons replacement
■ Required tools
■ Hexagonal keys
■ Ethanol or essence C
■2 hours
■ Frequency
■ See maintenance table

- XEA581BS: Piston replacement kit
- XEA485AS: 6 month maintenance kit
- XEA486BS: Yearly maintenance kit

the operations.
1. Kit XEA581BS

GBC030A 4 Reagent syringe piston
GBC031A 1 Lyse reagent syringe piston
GBG091A 1 Carriage,needle guide P60
GBG260A 2 Piston D=31 Spacer
GBG274A 2 Piston D=31
KAA010A 6 CHC M3x25 screws
Technical Manual
2. Sampling parts
(1 x XDA619BS + 1 x GBG091A)

ers\Maintenance carriage position.
■ Open the pneumatic access door (right side
of the instrument)
■ Unscrew the 2 fixation screws (2x CHC
M3x25) of the needle rinsing block on the
carriage.
■ Take out the needle and the rinsing block at

the same time (lift the needle locker to free
the needle).
■ Open the rinsing block assembly and XDA619BS GBG091A

replace the needle rinsing block (GBG091A)
and the needle (XDA619BS)
■ Reassemble following previous steps
backward.
■ When startup is done check for that there is
no leak FAA053A
■ Usea new FAA053A O’ring from 6 Month maintenance.

■ Usea dynamometric screw driver to tighten the Rinsing block, tightening torques:
120 mN.m (17 Ozf.in)
Technical Manual
3. Reagents syringe
1 x GBC031A & 4 GBC030A
■ Switch off the instrument, disconnect

the power supply cable.
■ Open the left side panel and the
mother board door (lock it) to access
to the syringe.
■ Disconnect the following tubes:
- valve 6 inlet 3.
- valve 7 inlet 3.
- valve 8 inlet 3.
- valve 9 inlet 3.
- valve 11 inlet 3.
■ Unscrew the 2 fixation screws and
carefully remove the reagent syringe.
■ Push several times the piston over

the waste container to drain the
syringes.
1
■ On a piece of absorbant paper, open
the reagents syringe (9x CHC M3x12
screws, 2x FX M3x12 screws).
■ Replace the pistons. 2
■ Replace at the same time all O’rings
and washers by new ones from the 6
month maintenance kit.
■ Use one drop of silicon grease
(LAM004A) in between 2 fingers to 8
lubricate the O’rings. 3
■ Apply a thin film of grease
(LAM004A) on the GBC030A and
GBC031A pistons.
■ Reassemble in reverse order:
1_Reagent syringe body (GBG033A). 7
2_XDA622A x4.
3_Piston (GBC030A) x4. 4
4_FX M3x12 screws x2. 6
5_CHC M3x12 screws x9.
6_Reagent syringe bottom plate.
7_Lyse reagent piston (GBC031A). 5
8_O’ring (FAA065A).
Technical Manual
Use a dynamometric screw driver to tighten the syringe, tightening torques:

- 9x CHC M3x12 : 400mN.m (56.8Ozf.in)
- 2x FX M3x12 : 400mN.m (56.8Ozf.in)
The thickness of the washer is matched to each O’ring thickness:

XDA622A = O’ring (FAA063A) + Washer (GBG149A).
For this reason, keep the O’ring and the washer together.
The lyse piston O’ring (small one) had no washer.
■ Remove any trace of oxydation on the

syringe holder with sand paper.
■ Grease
abundantly the syringe holder
(LAM004A).
■ Cleanthe endless screw with ethanol or
essence C (Do not grease the endless
screw).
Technical Manual
4. Draining & counting syringes
4.1. Draining syringe
(1x GBG274A + 1x GBG260A) or 1x

GBG052A)
■ Switchoff the instrument, disconnect the

power supply cable.
■ Open the right door to access to the syringe.
■ Disconnect the syringe electrical
connectors.
■ Unscrew the 4 CHC M4x16 syringe screws,
just few turns to release the silent blocks.
■ Put a piece of absorbant paper under the
syringe and disconnect all the tubes from the
syringe.
■ Remove the syringe.
■ Drain the syringe by pushing the piston nut up and down several times.
- 4x CHC M4x16 screws on old model.
- Rotation of the syringe body on new model.
Open
Close
The chamber body of the syringe is directly screwed on the motor block.
When the body is reinstalled on the motor block, it has to be rotated until the mark on the
body is located between the two marks of the motor block
Technical Manual
2
■ Prepare the piston: on the new molded old piston
syringe, the piston (1) is shorter.
■ For new molded syringe, install only the
piston GBG274A (1).
■ For old machined syringe, use the piston 1
GBG274A (1) + the cross piece GBG260A
(2) and use the CHC M3x20 screws from the
kit.
Use new O’ring from the 6 month maintenance kit.

O’ring (FAA017A) + Washer (GBG148A) = XDA621A
■ Use one drop silicon grease (LAM004A) in between 2 fingers to lubricate O’ring.
■ Apply a thin film of grease on the piston.
■ Make sure that the washer (GBG148A) is present on old model then reassemble in reverse order.
1_Motor assembly
2_O’ring (FAA017A)
3_Anti-extrusion ring (GBG219A)
4_Washer (GBG148A)
5_Syringe body
■ Clean the endless screw with ethanol or essence C (Do not grease the endless screw).
4.2. Counting syringe
■ Open the left side panel and the mother board door (lock it) to access to the syringe.
■ Follow the same procedure than for Draining syringe.
Technical Manual
5. Additional maintenance
■ The «Every 2 years maintenance» includes the «Yearly maintenance» and the «6 month maintenance».
■ Therefore, perform the RAS236C: 6 month maintenance and the RAS237C: Yearly maintenance
procedures.
■ Reassemble the covers then perform the «RAS252C: Check up after intervention» procedure to check
instrument accuracy and eventually re-calibrate it.
Technical Manual
RAS239C
Decontamination & rinse
■ Concerns
■ Instrument decontamination before maintenance

operations in the following cases:
- Instrument removed from biohazardous area
- Maintenance intervention on contaminated
assemblies
■ Required tools
■ Hexagonal keys
■ Clamps
■ Torx keys
■ Fungicidal, bacterial, virus killing detergent spray,
non corrosive for metals, non plastic altering.
■ Minoclair
■ 9° bleach
■ Deionized water
■ Distilled water
■ Absorbant paper
■ 1h30min.
■ Frequency
■ On request

■ None

the operations.
1. Preparation

■ Open the thermal door (Right side of the instrument).
■ Perform a Concentrated Cleaning from Menu: Maintenance\Hydraulics systems\Concentrated
cleaning.
■ When asked during the concentrated

cleaning cycle, poor 3ml of Minoclair in
each chamber, then click OK to continue.
■ When cycle is finished, switch the instrument off and remove the power supply cable.
■ Open the instrument covers.
■ Spray the bactericidal cleaner on all biohazardous areas (assemblies in contact with the biohazardous
material such as instrument cover, tube holder, keyboard...) and wait for 10 minutes.
2. Manual decontamination
■ Dilute the 9°Cl bleach to 1 part of bleach for 4 of deionize water (1/5).
■ Instrument environment must be cleaned and decontaminated.
■ No sponge, nor cloth must be used, only absorbant paper thrown after use in contamination bins, have
to be used. For small or sensitive assemblies use accurate drier papers.
■ All assemblies suspected to have been in contact with biohazardous material must be disinfected with
diluted bleach (the stainless steel must be bleached below 30°C).
■ Blood stains or salt marks must be cleaned with spray detergent first.
■ Concerned assemblies:
- Outer surfaces of the instrument (perpex, covers, reagent locations....)
- Waste connector plug
- Liquid valve push button
- Assemblies close to the needle
- Overflow trays
■ Reinstall all the assemblies and set up the instrument back to its initial configuration.
Technical Manual
3. Analysis circuit decontamination
■ Prepair 1 bottle containing 1/2 litre of bleach diluted to 1 part of bleach for 9 parts of deionize water
(1/10).
■ Prepair 1 bottle containing 1/2 litre of distilled water.
■ Replace the reagent bottles by the diluted bleach bottle.
■ Switch the instrument on.
■ Run 6 consecutive «All reagents» prime cycles (Menu: Reagents\Prime\All Reagents).
■ Fill a sample tube with diluted bleach to 1 part of bleach for 4 of deionize water (1/5)
■ Run 15 burn-in cycles (Menu: Technician\User mode\Burn-in).
■ Leave the instrument operating until it stops.
4. Drain & Rinse
■ Remove the reagent straws from the bottle containing the diluted bleach.
■ Wrap the straws in absorbant paper.
■ Run 2 consecutive «All reagents» prime cycles (Menu: Reagents\Prime\All Reagents): the bleach is
drained.
■ Replace the diluted bleach bottle by the distilled water bottle and re-plunge the straws in distilled
water.
■ Run 6 consecutive «All reagents» prime cycles (Menu: Reagents\Prime\All Reagents).
■ Remove the reagent straws from the distilled water (Wrap the straws in absorbant paper).
■ Run 2 consecutive «All reagents» prime cycles (Menu: Reagents\Prime\All Reagents): the distilled
water is drained.
■ Run a STAND BY cycle.
■ Re-install the reagent bottles and the straws.
■ Close the instrument cover.
Technical Manual
Technical Manual
RAS240C
LMNE flowcell adjustment
■ Concerns
■ LMNE Flowcell
■ Required tools
■ Hexagonal keys
■ Flatscrew driver
■ Torx keys
■ RBC/PLT Latex (LAD002DS)
■ Fresh blood sample
■ Calibrator
■1 h
■ Frequency
■ On request

■ None

the operations.
This adjustment procedure must be carried out when LMNE flowcell is moved or replaced, or when
LMNE tube is changed.
This adjustment must be checked in case of matrix quality trouble.
1. LMNE adjustment parameters
■ Menu: Maintenance\Technician\Measurement\LMNE Adjustment:

This menu allows the adjustment of the entire optical bench parameters. A 1/400th dilution of RBC/
PLT latex is automatically prepared (5µl of Latex, 2ml of diluent) in the LMNE chamber. This dilution is
then transfered toward the optical chamber and injected.
The measurements are continuously displayed every 700ms during a total of 27 seconds.
1.1. LMNE Lamp
Corresponds to the voltage on the LMNE amplifier board, between TP1 and TP2.
This voltage is defined by the light (from the lamp) received on the cell of the LMNE amplifier board.
Target value: 6,00 Volts +/- 0.50, adjustable with R11 on LMNE amplifier board.
Technical Manual
1.2. Transfer time
Time between the resistive detection of the cell in the micro aperture (1) and the absorbance detection
of the cell in the optical flow (2). This time is defined by the height of the optical flowcell, adjustable
with gauges.
Gauges
1.3. Resistive channel
This volumetric adjustment defines the size of the cells counted in the micro aperture of the LMNE
flowcell.
Target value: 53 +/-2, adjustable with R136, located on the mother board.
1.4. Absorbance channel
Focal position of the LMNE flowcell.

This adjustment defines the position of the LMNE flowcell between the emission gun, where the light
is sent by the lamp, and the reception gun, where the light is received by the cell of the LMNE amplifier
board.
Lamp Emission gun LMNE flowcell Reception gun
LMNE amplifier
LMNE ABS board cell
Technical Manual
2. Requirements
Before any adjustment, ensure that:

- The pneumatic circuit functions correctly.
- The transfer time is correct.
- The RA# on normal human fresh blood is correct (See Section 4, 3. Raw counts, page 4-17). A correct
RA# is around 90% of the number of WBC counted in the WBC chamber.
Technical Manual
3. LMNE Flowcell adjustment check.
Never dismantle the emission gun or even unlock it. The adjustment is factory made and
cannot be performed in the field.
■ Remove the upper cover and the left side

panel (see RAS247C: Covers dismantling).
■ Open the main board door (block it).
■ Unscrew the cover of the optical bench.
■ Run a Rinse cytometer cycle to get rid of air bubbles stuck to the inner optical surfaces:
■ Run Menu: Maintenance\Hydraulics systems\Rinse\Cytometer.
■ Check that the flowcell contains no or just a very few air bubbles.

■ Menu:
Maintenance\Technician\Mea-
surement\LMNE Adjustment:
■ Sample the Latex.
■ When optical bench is well adjusted:
■ Check that the displayed values are within the range in the following table.
Parameter Target Value Range

LMNE LAMP 6.00 5.50 to 6.50 V
TRANSFER TIME 200 150 to 250
RESISTIVE CHANNEL 53 51 to 55
ABSORBANCE CHANNEL 180 170 to 190
■ If you are out of range or to get a better adjustment follow the procedure.
Technical Manual
4. LMNE Flowcell position adjustment
■ Run a Rinse cytometer cycle to get rid of air bubbles stuck to the inner optical surfaces:
■ Run Menu: Maintenance\Hydraulics systems\Rinse\Cytometer.
■ Check that the flowcell contains no or just a very few air bubbles.
■ Visually check the position of the optical

flowcell:
■ If necessary, use the lateral screw (A) to ad- A
just the lateral position
■ The space between the emission gun and

the optical flowcell should be around 3 mm
(this may be checked using a 3 mm hexag-
onal key).
3 mm
■ If necessary, adjust this position by turning

the axial toothed wheel (B).

■ Menu: Maintenance\Technician\Measurement\LMNE Adjustment
■ Sample the Latex.
Technical Manual
■ Connect a voltmeter between TP1 and TP2

on the optical bench amplifier board.
■ During the cycle, adjust LMNE lamp voltage
around 6V by turning R11 potentiometer lo-
cated on this board.
■ Ifnecessary, adjust the Resistive gain (Re-

sistive channel) to 53 +/-2 by turning R136
located on the mother board.
■ First,
turn the lateral screw (A) to obtain a A
stable transfer on the LMNE adjustment
screen.
■ Then turn the axial toothed wheel (B) to ob-

tain the maximal absorbance.
■ Adjust again the lateral screw (A), then the
axial toothed wheel (B) to get a better Ab- A
sorbance. B
■ If
necessary, run the «LMNE adjustment»
again.
■ Run several cycles on normal human fresh blood and check if the CO% (See Section 4, 3. Raw counts,
page 4-17) may be increased by slightly turning the axial toothed wheel (B).
Technical Manual
5. Final adjustment
■ Check and adjust the matrix position as follow:

■ Select the raw counts: Enter in Setting/System setting/Printer menu and select «Raw values».
■ Run 5 different normal human fresh bloods and print the results.
■ Check the raw count on printout (See Section 4, 3. Raw counts, page 4-17):
- RA #: Number of cells counted. The RA# allows to check the pneumatical circuit from the sampling
to the LMNE flowcell. If the RA# is under expected value (around 90% of the WBC number), check the
pneumatic circuit first.
- CO%: % of cells crossing the flowcell in the correct time (transfer time) between the aperture and the
optical measurement. A correct CO% should be >95%.
- DIFF#: number of impact in LYM + MON + NEU + EOS population areas
■ Check the position of the populations on the matrix: the populations must be correctly
placed in their respective zone (box), without flags.
■ Check that the LYA and GCI values are as lower as possible (average maximum 1,3%).
■ Check that no alarm is triggered.
■ If necessary, adjust the matrix position:

Optical axis
Resistivity axis
1- Resistive gain adjustment:
- The resistive gain is set by R136 on the mother board.
- According to the resistive gain adjustment, populations of cells will move along the resistive axis in
different proportions according to the population:
- If the resistive gain is increased, cell populations will move to the right of the matrix.
- If the resistive gain is decreased, cell populations will move to the left of the matrix.
■A good adjustment is obtained when the Lymphocyte population is correctly centered in the LYM box.
2- Optical gain adjustment:

- The optical gain is set by R148 on the mother board (near R136).
- According to the optical gain adjustment, populations of cells will move along the optical axis in
different proportions according to the population:
- If the optical gain is increased, cell populations will move upward on the matrix.
- If the optical gain is decreased, cell populations will move downward on the matrix.
■A good adjustment is obtained when the Lymphocyte population is close to the NL threshold.
■ Run the 5 normal human blood again to check the correct adjustment of the matrix.
Technical Manual
6. Transfer time adjustment
■ Run this procedure in case of Transfer time out of range only (target value: between 150 and 250).
■ In case of replacement of an optical flowcell, a gauge set may be necessary to adjust the transfer time.
■ This gauge set (GBG264A) is now available as service part.
Before adjusting the height of the flowcell, make sure the other parameters are correct (lamp
voltage, resistive gain, LMNE Abs gain).
■ Afterreplacing the optical flowcell, if the

transfer time is too short, remove a gauge
from the set.
■ Ifthe transfer time is too long, add a gauge
from the set.
■ To add or remove a gauge, remove first the

two fixations, remove a gauge from the set,
re-install the set then block it with the fixa-
tions
■ Put the Optical bench cover back.
After intervention,check with normal human fresh blood that you have a correct matrix.
Technical Manual
Technical Manual
RAS241C
Chambers adjustment
■ Concerns
■ Chambers check and alignment
■ Required tools
■ Hexagonal keys
■ Gauge set MAJ004A
■ None
■ 15 mn
■ Frequency
■ On request

■ None

the operations.
Chambers adjustment
■ Itis mandatory to perform the «RAS242C: Needle adjustment» procedure after this
adjustment.
■ This adjustment procedure must be carried out when the chamber assembly is moved or
replaced.
1. Alignment check
■ Switch off the instrument and disconnect the power supply cable.
■ Open the right access door.
■ Install a gauge over the Rinse chamber then

move the carriage over the gauge.
■ Gently push down on the needle until it
comes just in contact with the gauge.
■ Without lifting the needle, gently push the

carriage over the WBC/BASO chamber.
■ Check that there is the same height
between the needle and the gauge over
WBC/BASO chamber and over Rinse
chamber.
Technical Manual
Chambers adjustment
2. Alignment adjustment
■ If
you need to get a better position, free the
chamber support by loosening the 6
screws.
■ Install
the gauge over the Rinse chamber
then move the carriage over the gauge.
■ Gently
push down on the needle until it
comes just in contact with the gauge.
■ Tighten gently the left upper screw.
■ Without lifting the needle, gently push the

carriage over the WBC/BASO chamber
■ Check that there is the same height
between needle and Rinse and WBC/BASO
tops of chambers.
■ If
necessary adjust the position by moving
up and down the chamber support.
■ Perform an alignment check as described
at the beginning of this procedure.
■ When adjustment is correct, tighten screws
and perform a RAS242C: Needle
adjustment procedure.
Technical Manual
Chambers adjustment
Technical Manual
RAS242C
Needle adjustment
■ Concerns
■ Check and adjustment of the needle
■ Required tools
■ None
■ None
■ 15 mn
■ Frequency
■ On request

■ None

the operations.
Needle adjustment
1. Introduction
Check that the chamber assy is parallel to the carriage motion before checking or making an
adjustment of the Needle position. Follow RAS241C: Chambers adjustment procedure of this
manual.
Most of the needle adjustments can be accessed by the menu «Maintenance\Technician\Dilution»»

as explained below:
Needle Home: Check (1): Allows to check Check (2): Checks the
Adjustment to get the best needle cleaning position deepness of the needle
needle extremity cleaning in front of LMNE-3 inlet
Carriage Home:
Allows adjustment of the
carriage sampling position
Needle position:
needle deepness
up & down adjustment
All Carriage pos.:

needle Left/Right
Check cycle: Allows to check that needle
adjustment
is aligned with each distribution inlets
Before any adjustment, check Needle position by running Check functions.
Technical Manual
Needle adjustment
2. Needle home check & adjustment
When the needle is in «NEEDLE HOME» position, it is also in cleaning position.

Check and adjust the NEEDLE HOME position to achieve the best cleaning of the needle
extrimity
2.1. Needle Home Check
■ Open the right access door

■ Run Maintenance\Technician\Dilu-
tion\Check (1).
■ Check that the needle is about 9.4mm out
from the carriage, compare with a 9.4mm
piece of paper that you have previously pre-
pared.
2.2. Needle Home Adjustment

■ Run Maintenance\Technician\Dilution\Check (1).
■ Check that the needle is about 9.4mm out from the carriage, compare with a 9.4mm piece of paper
that you have previously prepared.
■ If needle is too low (gap>9.4mm) increase Needle Home number of step.
■ If needle is too high (gap<9.4mm) decrease Needle Home number of step.
■ If necessary adjust Needle Home value and re-run a Check (1) cycle to control the gap again.
Technical Manual
Needle adjustment
3. All carriage positions check & adjustment
To check the right position of the needle, you must see inlet as in «A» and not as in «B» (where
circles are not concentric).
A B
3.1. All Carriage Positions Check
■ Open the right access door

■ Run Maintenance\Technician\Dilu-
tion\Check cycle.
■ Check in front of the inlet the right location
of the needle: Disconnect the tube connect-
ed to the inlet, if the needle is well adjusted
you will see it as in the diagram:
■ The sampling needle moves over a chamber.

■ Press Esc key to move to the next chamber and continue until the end of the cycle.
■ When the cycle is ended check that no tube is disconnected.
3.2. All Carriage Positions Adjustment
■ Needle Up/Down adjustment is the same for all the chambers and cannot be modified from
one chamber to another (adjustment by the «NEEDLE POSITION» value)
■ Needle Left/Right position in the chambers can be adjusted separately for each chamber
(except the Rinse chamber).

■ Before any adjustment, check the needle position in each chamber ( Maintenance\Technician\Dilu-
tion\Check cycle).
Technical Manual
Needle adjustment
3.2.1. Left/Right adjustment:
■ Adjustneedle position for all the chambers

separately in the software cycle order
■ Ifyou want to modify only one of the posi-
tion, remind to increase or decrease the
next motion with the same number of steps,
not to lose the other adjustments.
.
os
g ep s.
arria e po
c iag
ASO carr
/B NE
BC LM
(2 )W (3)
s. s.
e po po
iag iag
e 2
carr rr
1 ca
DIL RB
C
(1) (4) 4
3
1
■ The right location for the needle is when you

see it through the inlet as in this diagram:
■ When the cycle is ended check that no tube
is diconnected
Technical Manual
Needle adjustment
3.2.2. Up/Down adjustment
■ If the needle is too high increase Needle po-

sition value.
■ If the needle is too low decrease Needle po-

sition value.
Technical Manual
RAS243C
Main board adjustment
■ Concerns
■ Main board adjustment
■ Required tools
■ Smallflat screw driver
■ Hexagonal keys
■ Voltmeter
■ RBC/PLT Latex (LAD002DS)
■ 30min
■ Frequency
■ On request

■ None

the operations.
1. Main board general view
■ The main board is located on the left hand side of the instrument.
■ Unscrew the 4 CHC screws and remove the left panel to access to the main board.
Technical Manual
2. Power supply check
■ The power supply test points are located at the bottom of the main board and are not adjustable.
■ Power supply voltage check (ground on TP44):
Designation Test point Target Tolerance

5 V Power supply TP40 5V +/- 0.15 V
12 V Power supply TP41 12 V +/- 0.5 V
-12 V Power supply TP43 -12 V +/- 0.5 V
Technical Manual
3. Motor current adjustment
■ The motor current test points are located on the left upper corner of the main board.
■ Check and if necessary adjust the motor currents according to the following table (ground on TP57)
Motor Test point Potentiometer Target Tolerance

Draining syringe TP5 R149 4.8V +/-0.05V
Counting syringe TP6 R150 4.8V +/-0.05V
Reagent syringe TP7 R151 4.0 V +/-0.05V
LMNE syringe TP8 R152 3.0 V +/-0.05V
Horizontal carriage TP10 R154 3.0 V +/-0.05V
Sampling syringe TP11 R155 2.0 v +/-0.05V
Needle carriage TP12 R156 4.5 V +/-0.05V
Technical Manual
4. Threshold adjustment
■ The threshold test points are located at the top of the main board.
■ Check and if necessary adjust the threshold voltages according to the following table (ground on TP57)
Threshold Test point Potentiometer Target Tolerance

PLT TP2 R159 300 mV +/-5 mV
LMNE CIS TP3 R160 650 mV +/-5 mV
LMNE OD TP4 R161 350 mV +/-5 mV
RBC TP13 R158 300 mV +/-5 mV
BASO TP14 R157 300 mV +/-5 mV
Technical Manual
5. Draining sensor adjustment
■ Run Menu: Maintenance\Technician\Sensor Check\Draining.

■ The utility empties the drain cell and the following message is displayed:
■ Adjust the voltage between TP52 and ground to 4.5Volts +/- 0.3 using R287.
■ Press the Esc key.
■ The utility then fills the sensor with the diluent and the following message is displayed:
■ Check that the voltage between TP52 and ground is less than 1 Volt.
■ The test point is located in the left lower corner of the main board.
Technical Manual
6. Transfer sensor adjustment
■ Run Menu: Maintenance\Technician\Sensor Check\LMNE Transfer.

■ The utility empties the drain cell and the following message is displayed:
■ Adjust the voltage between TP48 and ground to 4.5Volts +/- 0.3 using R286.
■ The utility then fills the sensor with the diluent and the following message is displayed:
■ Check that the voltage between TP48 and ground is less than 1 Volt.
■ The test point is located in the left lower corner of the main board.
Technical Manual
7. HGB blank adjustment
This adjustment is made with the right side door closed and under stabilised thermic
conditions (5 min at least after startup).
■ Run Menu: Maintenance\Technician\Measurment\HGB Blank Adjustment.
■ The utility carries out a rinsing cycle of the

WBC chamber and continuously displays
the voltage used by the converter. The ad-
justment cycle lasts for 20 seconds.
■ The adjustment is carried out with the R248

potentiometer (located on the right upper
corner of the mother board).
■ The final adjustment voltage is approxi-
mately 4.7 Volts +/- 0.05V.
■ Once adjustment is done, check correct
voltage between TP1 and ground.
Technical Manual
8. RBC\PLT gain adjustment
■A special cycle allows the dilution of the RBC/PLT LATEX. A special count programme carries out the
calculations of the mean volume in the predefined zones and displays them every 700 ms. The
duration of the measurement cycle is 21 seconds.
Mix RBC/PLT Latex thoroughly before sampling.
■ Run Menu: Maintenance\Technician\Measurement\RBC/PLT Gain.
■ Follow the instructions on the screen.
■ Two graphs allow a visual check of the ad-

justments.
■ The adjustments are made using R133 potentiometer for the RBC and R135 for the PLT.
PLT RBC
■ Target values (for LAD002DS)

- RBC = 76 +/- 1
- PLT = 107 +/- 1
Technical Manual
9. WBC\BASO gain adjustment
■ A special cycle allows the dilution of the RBC/PLT LATEX. A special count program carries out the
calculations of the mean volume in the predefined zones and displays them every 700 ms. The
duration of the measurement cycle is 21 seconds
Mix RBC/PLT Latex thoroughly before sampling.
■ Run Menu: Maintenance\Technician\Measurement\WBC/BASO Gain
■ Follow the instructions on the screen.
■A graph allows a visual check of the adjust-

ments.
■ The adjustment is made using R134 potentiometer.
WBC
■ Target values (for LAD002DS)

- WBC/BASO = 100 +/- 1
Technical Manual
RAS244C
Temperature adjustment
■ Concerns
■ Heating coil and Thermostatic compartment

temperature check & adjustment.
■ Required tools
■ Thermometer
■ None
■ 30 mn
■ Frequency
■ On request

■ None

the operations.
1. Reagent heating coil
1.1. Heating coil temperature check
■ Concerns the control of the Heating coil temperature (reagent heating system).
This control must be made with the right side door closed and under stabilised thermic
conditions (5min at least after startup)
■ Open the right side of the instrument and

plunge the thermometer probe deeply in the
LMNE chamber’s inlet 1:
Make sure you have plunged the thermometer probe deeply into the liquid.
■ Close the right side door.

■ Enter Menu: Maintenance\Technician\Heating Systems\Reagent heating system\Reference.
■ Enter 5 in «Run Cycle Number».

■ Run Check and check that temperature is
aroud 35°C +/-1°C every time you hear a
«bip».
■ Check is validated if you get during control at least 10 times the right temperature.
■ Ifan adjustment is required, when heating block has been replaced for example, perform
the Heating coil temperature adjustment procedure.
Technical Manual
1.2. Heating coil temperature adjustment
■ Enter Menu: Maintenance\Technician\Heating Systems\Reagent heating system\Adjustment
■ Check and eventualy change the CTN

value.
The Heating coil CTN value is located on the CTN wire, close to the main board, connected
on J31.
Be sure to read the right value, as the sticker may be up or down.
■A LED near the connector (DS7) shows the heating command:

- when lit off, heating is off
- when blinking, heating is on
- when lit on, heating is maximum
■ Make a Heating coil temperature check (See “1.1. Heating coil temperature check, page 2”).
Technical Manual
■ If you do not get the right temperature,

adjust it by the mean of the «Reference
temperature».
When «Reference temperature» increases, reagent preheating temperature increases.

It may be necessary to restart the entire system after a «Consigne temperature»
changement.
■ Make a Heating coil temperature check (See “1.1. Heating coil temperature check, page 2”).
■ If required change the «Reference temperature» value and check temperature again, until you get an
acceptable temperature.
Technical Manual
2. Thermostatic compartment
2.1. Thermostatic compartment temperature check
■ Concerns the control of the Thermostatic compartment temperature.
This control must be made with the right side door closed and under stabilised thermic
conditions (5min at least after startup)
■ Open the right side of the instrument and

put the thermometer probe in the CTN
sensor.
■ Enter Menu: Maintenance\Technician\Heating Systems\Thermostated Compartment \Refer-

ence.
■ Enter 5 in «Run Cycle Number».

■ Run Check and check that temperature is
aroud 35°C +/-1°C every time you hear a
«bip».
■ Click the «Run» button and check that temperature is aroud 35°C +/-1°C every time you hear a «bip».
■ Check is validated if you get during control at least 10 times the right temperature.
■ If an adjustment is required, perform the Heating coil temperature adjustment procedure.
Technical Manual
2.2. Thermostatic compartment temperature adjustment
■ Enter Menu: Maintenance\Technician\Heating Systems\Thermostated Compartment\Adjust-

ment.
■ Check and eventualy change the CTN

value.
The Thermostatic compartment CTN value is located on the CTN wire, close to the main
board, connected on J33.
Be sure to read the right value, as the sticker may be up or down.
■A LED near the connector (DS8) shows the heating command:

- when lit off, heating is off
- when blinking, heating is on
- when lit on, heating is maximum
■ Make a Thermostated compartment temperature check (See “2.1. Thermostatic compartment temper-
ature check, page 5”).
Technical Manual
■ If
you do not get the right temperature,
adjust it by the mean of the «Reference
temperature».
When «Reference temperature» increases, reagent preheating temperature increases.

It may be necessary to restart the entire system after a «Consigne temperature»
changement.
■ Make a Thermostated compartment temperature check as described at the begining of the procedure.
■ If required change the «Reference temperature» value and check temperature again, until you get an
acceptable temperature.
Technical Manual
Technical Manual
RAS245C
Vacuum adjustment
■ Concerns
■ Counting and draining syringe vacuum control and

adjustment.
■ Required tools
■ Barflex
■ None
■ 15 mn
■ Frequency
■ On request

■ None

the operations.
Vacuum adjustment
1. Draining syringe vacuum check
No adjustment available

■ Disconnect the tube on the draining syringe
and replace it by the Barflex tube:
■ Run Menu: Maintenance\Techni-

cian\Vacuum Check\Draining.
■ Check vacuum on the Barflex, vacuum
must be around 260mb and must be stable.
■ Disconnect the Barflex and connect back
the tube on the draining syringe.
■ When installed in altitude, some instruments can trigger some «Transfer Sensor Timeout»
alarms. This is due to the decreased atmospheric pressure.
■ In these conditions, the draining vacuum can lower down to about – 200mb/–210mb
instead of –260mb (20% less): this results in a too long transfer time of the solution
between LMNE chamber to T1-T2 segment before the flowcell.
■ In such cases, we recommend to replace some 1.02 tubing (P/n: EAE005AS) by 1.3 tubing
(P/n: EAE006AS) in the following locations:
1- from valve #4 / port 2 to «T2/2»: 1.02x130 replaced by 1.3x130.
2- from «T2/3» to bottom right of 5Diff syringe: 1.02x240 replaced by 1.3x240.
3- from top right of 5Diff syringe to valve #5 / port 1: 1.02x95 replaced by 1.3x95.
Technical Manual
Vacuum adjustment
1 2
2. Counting syringe vacuum check

■ Disconnect the lowest tube on the counting
syringe and replace it by the Barflex tube :
Technical Manual
Vacuum adjustment
■ Menu: Maintenance\Technician\Vacuum
Check\Counting\Check.
■ Check vacuum on the Barflex while the fol-
lowing screen is displayed, vacuum must be
at 220mb.
■ Disconnect the Barflex and connect back
the tube on the counting syringe.
3. Counting syringe vacuum adjustment
■ Check Counting syringe vacuum as previously described.
■ Menu: Maintenance\Technician\Vacuum
Check\Counting\Check.
■ Check vacuum on the Barflex, vacuum
must be at 220mb.
■ Press Esc key and change the step value. If
vacuum is too low increase step value.
■ Repeat vacuum check and step value adjustment until you get the correct vacuum.
■ Disconnect the Barflex and connect back the tube on the counting syringe.
Technical Manual
RAS247C
Covers dismantling
■ Concerns
■ Covers dismantling & mounting
■ Required tools
■ Hexagonal keys
■ Torx keys
■ None
■ 10 mn
■ Frequency
■ On request

■ None

the operations.
Covers dismantling
1. Dismantling

■ Disconnect the power supply cable.
■ Open the reagent door and remove the 4 bottles (disconnect tubes from the stoppers).
■ Open the pneumatic access door (right side of the instrument).
■ Unscrew and remove the left side panel (4

CHC screws).
■ Open the main board door (lock it).
■ Loosen the 2 screws and open the right

door.
■ The door may also be dismantling by
unscrewing the 3 CHC screws at the rear of
the door.
Technical Manual
Covers dismantling
■ Unscrewand remove the upper cover (7

CHC screws).
B
A
■ Make sure the needle is lift and push the
carriage backward.
■ Loosen the K30x16 torx screw (B).
■ Unscrew the 2 CHC M4x6 upper screws (A).
■ Unscrew the 4 CHC M4x10 screws in the

reagent compartment:
Technical Manual
Covers dismantling
■ Lift and pull a little the front panel, to free

space in order to disconnect the sampling
LEDs command in the internal side of the
panel:
■ Remove the front panel.
2. Mounting
■ Reassemble by following the previous procedure in reverse order.
■ Be careful not to clamp one of the reagent’s tube behind the reagent compartment.
Technical Manual
RAS248C
Heater assy replacement
■ Concerns
■ Replacement of the heater assy
■ Required tools
■ Hexagonal keys
■ Torx keys
■ None
■1 h.
■ Frequency
■ On request

■ Heater assy: XDB032BS

the operations.
1. Heater assy dismantling
■ Drain chambers (Menu: Maintenance\Hydraulics systems\Drain chamber\All chambers).

■ Move the carriage to the «Maintenance carriage position» (Menu: Maintenance\Technician\Oth-
ers\Maintenance carriage position).
■ Turn off the instrument.
■ Disconnect the power supply cable.
■ Remove covers (see RAS247C: Covers dismantling).
■ Pull the reagent syringe and the LMNE

syringe to free space behind (no need to
disconnect tubes). Unscrew several turns
the 4 silent block screws of each syringe
■ Remove the chamber «cover» (A):
Technical Manual
■ Disconnect the following tubings:

- «DIL1»
- «DIL2»
(Dil1 &DIL 2 pass through the first hole,
between Rinse and DIL1/HGB chambers).
- «LMNE4» from transfer sensor.

- «LMNE3»
(LMNE3 & LMNE4 pass through the second
hole, between DIL1/HGB and LMNE
chambers).
- «LMNE2»
(LMNE2 pass through the third hole,
between LMNE and RBC chamber).
- BASO2 (connector).
(BASO2 pass through the hole in the
chamber plate, on the right side of WBC/
BASO chamber.
■ Disconnect the following tubings:

- Valve 22-1
- valve 22-2
- valve 8-2
- «T 4»-1
- valve 11-2 (connector) B B
■ Remove the CTN from its support.
■ Loosen the 2 heater assy fixation screws
(B):
■ Unscrew the chamber plate screws (6
CHC):
■ Carefully open the chamber plate:
Technical Manual
■ Disconnect heating system wiring plugged

in J31 on the mother board:
■ Unscrew the 2 heater assy fixation screws

■ The heater assy is free, let it lay on the frame
and by the front aperture disconnect the
following tubes: 2 4 6 8 10 12
- From valve 22_2 (rep.1 on heater)
- From valve 8_2 (rep.3 on heater) 1 3 5 7 9 11
- From T4 (rep.5 on heater)
■ Remove the cable through the instrument.

■ Remove the heater assy and drain it over
absorbant paper:
Technical Manual
2. Heater assy replacement
■ Put the new heater assy on the instrument frame and connect following tubes:
From To
Heater assy rep n°1 Valve 22 Inlet 2
Heater assy rep n° 3 Valve 8 Inlet 2
Heater assy rep n° 5 T4
■ Dismantle chamber assy to get the tubes

through the chamber’s support:
■ Lift
the heater assy, make the tubes go
through the chamber’s support:
E
- Dil1 &DIL 2 pass through the first hole (A). D
C
B
- LMNE3 & LMNE4 pass through the A
second hole (B).
- LMNE2 pass through the third hole (C).
- RBC1 pass through the fourth hole (D).
- BASO2 pass through the fifth hole (E).
■ Screw back the 2 heater assy screws.

■ Screw back the chamber plate on the
frame:
Technical Manual
■ Put back the chamber assy and connect the following tubes on chambers:
- «DIL1»
- «DIL2»
- «LMNE4» (on transfer sensor)
- «LMNE3»
- «LMNE2»
- BASO2 (connector).
■ Install the CTN on its support.
■ Connect heating system wiring on the mother board in J33.
■ Put back the Reagents syringe and the LMNE syringe and screw silent blocks (check that no tube is
disconnected).
■ Put back the covers (see RAS247C: Covers dismantling).
■ Check chambers position (see RAS241C: Chambers adjustment).
■ When startup and prime are done check that there is no leak.
Technical Manual
RAS249C
Bubbling adjustment
■ Concerns
■ Bubbling adjustment.
■ Required tools
■ None
■ None
■ 15 mn
■ Frequency
■ On request

■ None

the operations.
Bubbling adjustment
1. Bubbling check and adjustment
■ Bubbling is factory adjusted and normally does not required further adjustment.
■ If an adjustment is necessary follow this procedure:
■ Enter Menu: Maintenance\Techni-

cian\Bubbling.
■ Enter new step value for each bubbling.
To increase bubbling, increase the number of steps.

To decrease bubbling, decrease the number of steps.
2. Bubbling values
Bubbling Low limit Normal Hight limit

FIRST DILUTION 100 300 400
LMNE 100 300 400
WBC/BASO 100 300 400
RBC/PLT 100 300 400
HGB 300 400 500
Technical Manual
RAS250C
Power supply replacement
■ Concerns
■ Power supply dismantling
■ Required tools
■ Hexagonal keys
■ Voltmeter
■ None
■ 30 mn
■ Frequency
■ On request

■ Power supply: DBN004

the operations.
1. Dismantling & Replacement

■ Disconnect power supply cable.
■ Open the left side panel.
■ Open the cover.
■ Disconnect , on the mother board, the
printer RS flat cable in J2.
■ With a flat screwdriver disconnect, on the

mother board, the supply connector in J37:
■ Open the left side panel and the main board

door (lock it).
■ Disconnect the Optical bench lamp supply
cable:
Technical Manual
■ Unscrew the 2 CHC M3x6 back screws of

the power supply:
■ Unscrew the 2 CHC M3x6 front screws of

the power supply.
■ Remove the power supply.
Be careful to the lamp power supply cable when you remove the power supply.
■ Reassemble by following the previous steps backward.
Technical Manual
2. Power supply check

■ The power supply test points are located at the bottom of the main board and are not adjustable.
■ Power supply voltage check (ground on TP44):
Designation Test point Target Tolerance

5 V Power supply TP40 5V +/- 0.15 V
-12 V Power supply TP43 -12 V +/- 0.5 V
Technical Manual
RAS251C
■ Concerns
■ Start cycle microswitch replacement
■ Required tools
■ Hexagonal keys
■ Torx key
■ None
■ 30 mn
■ Frequency
■ On request

■ None

the operations.
1. Introduction
■ The start cycle microswitch has to be changed or dismantle only when required
■ To access to the start cycle microswitch you need to remove the Front panel (see RAS247C: Covers
dismantling).
■ Open the pneumatic access door (right side of the instrument).
2. Start Cycle Microswitch Dismantling
■ Unscrew the 2 CHC screws maintening the

thermostated compartment fan (the ground
is screwed with a washer on the lower
screw).
■ Remove the fan (not necessary to
disconnect).
■ This allow the access to the microswitch
screw.
■ Release the start cycle microswitch by

loosening the 2 screws.
Technical Manual
■ Disconnect the start cycle microswitch.

■ Pick up the 2 screws and the fixing plate (A).
3. Start Cycle Microswitch replacement
■ Connect the new Start cycle microswitch.
The microswitch is connected Normally Open.
■ Install
the fixing plate (A) previously
dismantled on this switch.
■ Put back the start cycle microswitch in the
2 slots (nearly full backward).
■ Tighten the 2 microswitch screws.
■ Check with the front panel in its location, that the Start cycle key is operating normally. If not move
forward or backward the microswitch until you get a correct location.
■ You must ear a «click» when key is pressed, and one when key is released.
■ Reinstall fan.
Use the washer to connect ground wiring to the fan.
■ Reinstall covers.
Technical Manual
Technical Manual
RAS252C
■ Concerns
■ Check up and control of the instrument accuracy
■ Required tools
■ None
■ Fresh and normal blood samples
■ Calibration blood samples
■ 45 mn
■ Frequency
■ On request

■ None

the operations.
1. Preliminary
This procedure must be performed on a clean instrument.

If the instrument is suspected to not be perfectly clean, perform a Concentrated cleaning.
■ Start the Analyzer.

■ Run a startup.
2. Repeatability
■ Based on 20 consecutives analyses without alarm from one fresh and normal blood sample.
■ Run 20 consecutives analyses.
■ Control to have variation coefficients within the following acceptable limits.
Parameters %CV Test level

WBC <2% at 10x103/µL
RBC <2% at 10x106/µL
HGB <1% at 15 g/dL
HCT <1% at 45%
PLT <5% at 300x103/µL
2
Xi X – Xi SD
X = ------------ CV = ------- 100
n SD = ------------------------------
n–1 X
X: Mean
Xi: Measure value
n: Measure number
SD: Standard deviation
■ With one fresh and normal blood sample check that there is no alarm in CBC+5DIFF mode.
Technical Manual
3. Calibration
■ Enter Menu: Calibration\Autocalibration.
■ If necessary, change Lot number, Expiration date or Target values according to the lot you are going
to use.
■ Prepare the calibrator according to the specific instructions detailed in the calibrator package insert
(temperature, mixing, etc...).
■ Open the vial and position the sampling needle deeply inside the bottle
■ Press the sampling bar located behind the needle
■ When the cycle LED stops flashing, remove the vial and replace the cap on the calibrator.
■ Risk of erroneous results if the calibrator is not continously mixed between each analysis.
Continue mixing the calibrator between each analysis.
■ The calibration can be performed on 3 to 11 analyses. In order to obtain the best possible
calibration, it is recommended to run at least 5 calibrator samplings.
■ When the analysis cycle ends, the first result is displayed on the result chart table.
■ Run another calibrator sample when Green LED turns on.
■ The auto-calculation module performs

statistics on these results in order to obtain
the best calibration coefficients.
Technical Manual
■ If the statistic figures are within the

acceptable limits:
- Coefficient of variation is within the limits
setup as described section and
- The percentage difference between the
target and the mean value is less than 20
■ Press Enter to save the new coefficients
■ Press Enter to print the new coefficients
■ Check that the calibration coefficients remain within the following ranges:
Calibration Coefficient Standart Value Minimum Maximum

WBC 137 90 200
RBC 225 160 290
HGB 40.0 25.0 55.0
HCT 220 160 290
PLT 290 180 400
RDW 0.3 0.1 0.9
Technical Manual
RAS253C
Step by step analysis cycle
■ Concerns
■ Analysis cycle description
■ Required tools
■ None
■ None
■ 15 mn
■ Frequency
■ On request

■ None

the operations.
1. Introduction
■ The main cycle consists of four principal phases:

1- Preparation of the sample phase: dilutions
2- Count and measurement of the WBC group
3- RBC/PLT group count
4- Filling of tank
■ A special cycle is required when no other cycle is started during RBC/PLT count or during the filling
up of the tank:
5- Rinsing of the RBC/PLT count chamber
2. Start cycle condition
■ Startup cycle OK.

■ Needle in sampling position and green LED lit.
■ All chambers (except Rinse chamber) filled with ABX Diluent.
■ Reagents syringe & LMNE syringe down.
■ Draining & Counting syringe down.
3. Chambers
Technical Manual
4. Step by step analysis cycle
4.1. Preparation of the sample phase: dilutions
4.1.1. First series of dilutions:

■ Press sampling bar, all the chambers are drained (draining syringe).
■ Blood sample of 53 µl (sampling syringe).
■ Translation of the needle toward the Rinse chamber (sampling needle motor, carriage motor).
■ Distribution of 3 µl of blood during the rinsing of the needle (sampling syringe, reagents syringe).
■ Transfer of the needle from Rinse chamber to the Dil1/HGB chamber (sampling needle motor, carriage
motor).
■ Distribution of 10 µl of blood into a flux of 1.7 ml of ABX Diluent (sampling syringe, reagents syringe).
■ Transfer of the needle from the Dil1/HGB chamber to the WBC/BASO chamber (sampling needle
motor, carriage motor).
■ Distribution of 10 µl of blood into a flux of 2 ml of ABX Basolyse 2 (sampling syringe, reagents syringe).
■ Transfer of the needle from the WBC/BASO chamber to the LMNE chamber (sampling needle motor,
carriage motor).
■ Distribution of 25 µl of blood into a flux of 1 ml of ABX Eosinofix (sampling syringe, reagents syringe).
■ Transfer of the needle from the LMNE chamber to the Rinse chamber (sampling needle motor, carriage
motor).
■ Distribution of the 5 µl of residual blood during the double rinsing (interior and exterior) of the needle
(sampling syringe, reagents syringe).
4.1.2. Realisation of the LMNE dilution

The dilution in the LMNE chamber is completed by 1 ml of ABX Diluent after 12 seconds of incubation:
■ Positioning of the needle opposite the distribution nipple for the EOSINOFIX.
■ Control of valve 8 then set the syringe group for a dose of 1 ml.
■ Simultaneously: setting of the distribution syringe to distribute 25 µl of blood.
■ Stop valve 8.
■ Incubation for 12 seconds.
■ Control of valves 1 and 3 and setting of the LMNE injection syringe to distribute 1 ml of the stop diluent.
■ Stop valves 1 and 3.
4.1.3. Second dilution for the RBC and the PLT (for instrument NON
equipped with Takasago valves):
■ Transfer of the needle from the Rinse chamber to the Dil1/HGB chamber (sampling needle motor,
carriage motor).
■ Sample of 42.5 µl of the first dilution (sampling syringe).
■ Rinsing of needle exterior (in the Dil1/HGB chamber) with 0.4 ml of ABX Diluent (reagents syringe).
■ Transfer of the needle from the Dil1/HGB chamber to the RBC chamber (sampling needle motor,
carriage motor).
■ Distribution of the 42.5 µl of first dilution into a flux of 2 ml of ABX Diluent (sampling needle motor,
carriage motor).
Technical Manual
■ Distribution of 0.5 ml of ABX Diluent from the interior of the needle (sampling needle motor, carriage
motor).
■ In the case of this RBC/PLT dilution, it is the entire blood sample that is distributed (and not
a portion as in the preceding dilution). The distribution in a flux is kept in order to benefit
from the homogenisation aspect of the procedure.
■ In order to attain the hemoglobin measurement we add 0.4 ml of lysing agent into the first
dilution chamber immediately following the blood sample for the RBC/PLT dilution.
4.1.4. Second dilution for the RBC and the PLT (for instruments equipped
with Takasago valves):
■ Valves 32 and 33 commute (ON).
■ Valves 9 & 10 commute (ON).
■ Aspiration of a part of the first dilution through EV32 & 33 by the mean of the Diluent reagent syringe
(the lenght beetwen EV32 and EV33 gives the exact volume for the RBC sample).
■ The sample (42 µL) is pushed into RBC chamber with a flux of 2.2 mL of ABX Diluent by the mean of
the Diluent reagent syringe through valves 9 & 10.
■ Bubbling by the mean of the Draining syringe through valve 30 (ON).
4.2. Count and measurement of the WBC group
■ LMNE: Loading of the vacuum injection circuit of the optical bench (draining syringe, control of valves
4 and 5).
■ Start flux in the optical chamber (approximately 2 seconds).
■ Injection, acquisition of the matrix and WBC count for 12 seconds.
■ Simultaneously: WBC and BASO counts: Rinsing of the counting head (diluter pushed into position,
control of valves 9, 10, 21, 23 and 13).
■ Start the vacuum and first part of count (duration of 6 seconds, control of valve 23).
■ Rinsing of counting head and adjustment of the vacuum count.
■ Second part of count (duration of 6 seconds).
■ After this first series of measurements:
- Measurement of the hemoglobin.
- Rinsing of the WBC/BASO chamber with a mixture of 1 ml of ABX Cleaner and 1.5 ml of ABX Diluent.
- Rinsing of the WBC/BASO counting head.
4.3. RBC/PLT group count
■ Rinsing of the RBC/PLT counting head. Start the vacuum for the RBC/PLT counting head.
■ First series of counts (duration of 5 seconds, control valve 14).
■ Rinsing of the RBC/PLT counting head and adjustment of the vacuum.
■ Second series of counts (duration of 5 seconds).
Technical Manual
■ P.R.F. Process (Permanent Rinse Flow):

The WBC/BASO and the RBC/PLT counting heads are «in series» during the RBC/PLT count.
The count flux from the WBC/BASO chamber circulates in the RBC/PLT counting head and
limits the possibility of the circulation of the RBC behind the count orifice.
■ Autocleaning of the WBC/BASO micro aperture:
The P.R.F. allows an auto cleaning of the WBC/BASO micro aperture inasmuch as aABX
Cleaner/ABX Diluent (cleaning solution) mixture is aspirate through the WBC/BASO micro
aperture during the entire duration of the RBC/PLT counting.
4.4. Filling of tank
■ At the end of the RBC/PLT count, the RBC count vacuum is «finished» and used for the filling up of the
diluent tank (control valves 15 and 25).
■ In order to improve the filling speed, the syringe is initialised for a few seconds then drawn again to
complete the filling.
■A float located in the tank controls the level of liquid.
4.5. Rinsing of the RBC/PLT count chamber
■ For reasons of maximising the mechanical resources, the rinsing of the RBC/PLT chamber is
«normally» programmed for the beginning of the succeeding cycle (which can be started during the
RBC and PLT count).
■ If no other cycle is started during RBC/PLT count or during the filling up of the tank, a special rinsing
sequence is initiated. The sample signal light changes to red and the instrument is «jammed» for a
few seconds.
■ This sequence involves draining of the RBC chamber followed by a refilling with the diluent.
Technical Manual
Technical Manual
RAS254C
Optical bench dismantling & replacement
■ Concerns
■ Dismantling, Replacement and Control of Optical

Bench
■ Required tools
■ Hexagonal keys
■ None
■ 30 min.
■ Frequency
■ On request

■ None

the operations.
1. Dismantling & Replacement

■ Disconnect power supply cable.
■ Open the pneumatic access door (Right side of the instrument).
■ Remove covers (see RAS247C: Covers dismantling).
■ Open the mother board door (lock it).
A flat cable is connected at the back of the mother board, becareful when you open the
door that you don’t disconnect or damage it.
C
■ Disconnect tubes from LMNE syringe: D
- Inlet 1 (A)
- Inlet 5 (B)
■ Disconnect tubes from valves:
- 1 Inlet 1 (C)
- 4 Inlet 2 (D)
A
B
■ Disconnect tube from ground fitting
Technical Manual
■ Disconnect following connections:

- Lamp supply.
- LMNE CIS & LMNE OD coaxes.
- Optical pre-ampli board supply.
- Optical bench grounding wire.
■ Untighten the silent blocks (5mm allen key):

■ Gently remove the Optical bench.
■ Locate new Optical bench.

■ Tighten the silent blocks (5mm allen key).
Do not tighten silent blocks too hard, check that optical bench cannot be lifted.
■ Connect all tubing and wiring as previously disconnected.
Technical Manual
2. Control
2.1. Emission gun location control
■ Open Optical bench cover:
■ Check that gap between emission gun and

flowcell is around 3mm:
■ Be careful, if you use a 3mm allen key to control gap, not to damage the flowcell or the
emission gun.
■ This adjustment is factory made and must not be changed.
2.2. Optical bench lamp alignement control
see "3.1. Lamp position check", page 6
Technical Manual
3. Lamp replacement
■ Switch off the instrument and wait for the lamp to cool down for at least 10 minutes.
As for halogen/quartz lamp, do not touch the bulb with your fingers. This will reduce
significantly the shelf life of the lamp. In case of finger contact, clean the bulb with a solution
of 90% alcohol and a soft paper.
■ Access to the optical bench as previously

described.
■ Open Optical bench cover.
■ Disconnectthe Optical bench lamp power

supply connector.
■ Loosen the 2 CHC screws of the lamp
holder .
■ Turnthe lamp holder then remove the lamp
+ holder from the optical bench.
■ Remove the holder from the lamp.

■ Install the holder on the new lamp. Holder
■ Install
the lamp + holder on the optical
bench then tighten the 2 CHC screws.
Technical Manual
3.1. Lamp position check
■ Connect the power suplly cable then turn

on the instrument.
■ To control the lamp, put a piece of white
paper between reception gun and flowcell:
■ Make sure the flowcell is full of liquid, if not run a «Rinse Diluent» cycle.
■ Make sure that there is no bubble inside the flowcell.
■ Make sure of the cleanliness of the flowcell optical window and objectives.
■ Check the projection of the lamp filament

on the paper.
- You must see the projection entirely.
- The turns of the filament must be well-
defined.
Technical Manual
3.2. Lamp position adjustment
■ If
the projection of the filament is not correct, as shown on previous picture, adjust the lamp support
position: (a small screw, 1.2mm allen key, allows adjutment of lamp).
■ Loosen the small screw.

■ Adjust the lamp position (rotation) to have a
correct projection of the filament on the
paper.
■ Remove the paper, then connect a
voltmeter on the LMNE amplifier board,
between TP55 (ground) and TP53.
■ Adjust the height position of the lamp
support to have the maximum voltage on
the voltmeter.
■ Check the projection of the filament again,
then block the support by screwing the
small screw.
■ Adjust the Lamp voltage to 6 +/- 0.5 V with
the R414 potentiometer on the LMNE
amplifier board.
Technical Manual
Technical Manual
RAS255C
External barcode reader configuration
■ Concerns
■ Barcode Reader test and configuration
■ Required tools
■ None
■ None
■ 15 mn
■ Frequency
■ On request

■ None

the operations.
1. Barcode reader configuration check
■ Check that the barcode reader is working properly by means of the following reading test.
INTERLEAVED 2 OF 5 (no «check digit» control)
with C.D.
1224488
reading12244881
without C.D.
121314151617
reading 121314151617
CODE 39 (no «check digit» control)
with C.D.
reading 12345ABCDE
without C.D.
reading 12345ABCDEW
CODE 128 (control without «check digit» transmission)
12345abcde QWERTY
12345ABCDE azerty
CODABAR (no egality control between start/stop character)
37859 123456/$
reading 37859 reading 123456/$
Technical Manual
2. Barcode reader setup
■ An audible beep occurs after each reading. Read all the labels from the top to the bottom.
■ When the last label is read, check once more on the test labels.
For the code I2 of 5, to avoid a bad interpretation, it is mandatory to hold the reader in order
to read the entire code.
■ If the reading test has failed for the DATALOGIC reader, proceed to the barcode setup as follows:
$+$* Restore system default configuration
$+CP0$- RS232 interface
$+ Enter configuration environment
CA0CC1CE2 7 data bits, parity even, XON/

XOFF
AZ0 Disable all family codes
AB11AB*0ll6 Code39, no C.D., 1 to 16

characters
AC110416 ITF, no C.D., 4 to 16 characters
AD111AD*0316 Codabar, no start/stop, 3 to

16 characters
AI11 Code128, C.D. control without

transmission
EA110DEC0 Terminator = CR, FIFO

disabled
ED3 4 good reads before accepting code
$- Exit and save configuration
Technical Manual
Technical Manual
RAS304C
LMNE balance adjustment
■ Concerns
■ LMNE Balance calibration and forced calibration.
■ Required tools
■ None
■ Fresh and normal blood samples (5 different
samples at least)
■ 15 min
■ Frequency
■ On request

■ None

the operations.
This procedure must be performed on a clean instrument.

If the instrument is suspected not to be perfectly clean, perform a Concentrated cleaning.
■ Start the analyzer.

■ Run a Startup (If not performed automatically).
1. Calibration
■ Enter Menu: Maintenance\Techni-

cian\WBC Matrix Calibration
■ Prepare a minimum of 5 different human blood samples without WBC alarm.

■ Run one of the samples.
■ When the analysis cycle ends, the first result is displayed on the result chart table.
■ When the Green LED turns on, run another specimen, and so on until you get a minimum of 5 different
results.
At least 5 results are necessary to calibrate the LMNE Balance.
■ The results selected to be involved in the

statistical calculation have a dot in the
square column. To discard a result from the
statistical calculation, select the line and
press the DOT key.
Technical Manual
■ When ready to calibrate press ENTER to

apply new calibration coefficient
■ Enter user password to confirm calibration
■ When calibration is ended press ESCAPE to

exit.
■ The calibration values are printed out
If the variation between the previous and the new coefficients exceeds 20%, the calibration
fails.
LMNE Balance coefficient range: 0,5 to 1,5
Technical Manual
2. Forced calibration
■ Enter Menu: Calibration\Change coeffi-

cient.
■ With Up and Down arrows select the WBC
LMNE calibration coefficient and change it
Technical Manual
RAS680A
■ Concerns
■ Takasago valve assembly dismantling:

- for chamber or heater access
- for Takasago valves replacement
■ Required tools
■ Hexagonal keys
■ Cruciform screw driver
■ None
■ 15 mn
■ Frequency
■ On request

■ None

the operations.
1. Purpose
Because of Takasago valve, it could be more difficult to access to the chambers or to the heater
assembly.
Follow the first part (“2.Chamber access, page 2”) of this procedure to remove the Takasago valves for
maintenance on those assemblies.
Follow the second part of this procedure (“3.Valve assembly replacement, page 3”) for the Takasago
valve assembly replacement or for one valve dismantling only.
The second part of the procedure has to be used in case of failure of the Takasago valve
assembly only.
2. Chamber access
2.1. Disassembly
■ Run Menu: Maintenance\Hydraulics systems\Drain chamber\All chambers to drain the chambers.
■ Turn off the instrument and disconnect the power supply cable.
■ Open the right door.

valve plate on the crosspieces.
■ Carrefully disconnect the small tubing

(magenta on picture) FROM THE VALVE
MANIFOLD ONLY in order not to damage
the chamber inlet.
■ In this configuration, it is possible to move
the valve assembly in order to work on
chambers.
Technical Manual
RAS680A - 2 RAA016CEN
2.2. Reassembly
■ Connect the short tubing (from the DIL1/

HGB 4 inlet, magenta on the picture) on the
manifold.
■ Locate the tubings (yellow on the picture)
out of the crosspieces, as shown on the
following picture.
■ Install
the valve plate on the crosspieces
using the 2 CHC screws.
3. Valve assembly replacement
■ Run Menu: Maintenance\Hydraulics systems\Drain chamber\All chambers to drain the chambers.
■ Turn off the instrument and disconnect the power supply cable.
■ Open the right door.

valve plate on the crosspieces.
Technical Manual
RAA016CEN RAS680A - 3
■ Carrefully disconnect the small tubing

(magenta on picture) FROM THE VALVE
MANIFOLD ONLY in order not to damage
the chamber inlet.
■ Disconnect the long tubing (green on the

picture) from RBC1.
■ Disconnect the «T» connector from

Reagent heater 8 tubing (blue on the
picture).

■ Disconnect the two electrical connectors
from the extension cable, then remove the
valve assembly.
■ Reassemble in reverse order. Take care to the tubing position (See “2.2. Reassembly, page 3”).
Technical Manual
3.1. Valve dismantling
■ Cutthe tyraps maintening the valve wirings

together.
■ Unscrew the 2 CHC screws (A) to remove A
the valves + manifold from the plate.
■ Unscrew the 2 cruciform screws (B) to B
remove the valve from the manifold. Take
C
care to the seal (C).
Technical Manual
RAA016CEN RAS680A - 5
Technical Manual
Exploded views
8 - Exploded views
1. List ...........................................................................................................................2
2. Valves ......................................................................................................................6
3. Takasago valve assembly .......................................................................................7
4. 5 Diff syringe ...........................................................................................................8
5. Sampling syringe .....................................................................................................9
6. Reagents syringe ..................................................................................................10
7. Waste syringe ........................................................................................................11
8. Counting syringe ...................................................................................................12
9. Diluent tank assy ...................................................................................................13
10. Rinse block & needle ..........................................................................................14
11. Carriage assy ......................................................................................................15
12. Carriage peripherals ............................................................................................16
13. Optical bench ......................................................................................................17
14. Chambers for instrument NON equipped with Takasago valves ........................18
15. Chambers for instrument equipped with Takasago valves .................................19
16. Chamber parts ....................................................................................................20
17. Thermostated room ............................................................................................21
18. Electronic boards ................................................................................................22
19. Straws & tubes ....................................................................................................23
20. Reagent connection assembly ............................................................................24
21. Miscellaneous .....................................................................................................24
22. Power supply ......................................................................................................25
23. Covers .................................................................................................................26
Technical Manual
RAA016CEN 8-1
Exploded views
List
1. List
N° in
Drawing Reference Designation
drawing
“2.Valves, p 6” 1 XDB123A VALVE, LIQ 11 VALVE ASSY P60 (1 to 11)
“2.Valves, p 6” 4 XDB126A VALVE, LIQ 7 VALVE ASSY P60 (20 to 26
“2.Valves, p 6” 6 XDB135A 2W VALVE WITHOUT COIL
“2.Valves, p 6” 7 XDB136A 3W VALVE WITHOUT COIL
“3.Takasago valve assembly, p 7” 1 XBA685A VALVE, TAKASAGO + MANIFOLD ASSY
“3.Takasago valve assembly, p 7” 2 XBA646AS TAKASAGO VALVE
“3.Takasago valve assembly, p 7” 3 EZZ003A TAKASAGO VALVE MANIFOLD
“3.Takasago valve assembly, p 7” 4 XEA960AS KIT, TAKASAGO KIT O'RING + SCREW
“3.Takasago valve assembly, p 7” 5 XEA959AS TAKASAGO WIRING KIT
“4.5 Diff syringe, p 8” 1 GBG037A SYRINGE, 5DIFF BLOC BODY P60/80
“4.5 Diff syringe, p 8” 2 FAA040A O'RING, 5DIFF SYRINGE D=12,1
“4.5 Diff syringe, p 8” 3 FAA067A O'RING, 5DIFF SYRINGE D=2,4
“4.5 Diff syringe, p 8” 4 GBG042A SYRINGE, CROSSPIECE P60/SPS/P80
“4.5 Diff syringe, p 8” 5 GBG040A SYRINGE, 5DIFF PISTON P60/P80
“4.5 Diff syringe, p 8” 6 XDA616AS NEEDLE, 190µL SYR. P60/SPS/P80
“4.5 Diff syringe, p 8” 7 XDA591AS SYRINGE, 5 DIFF SYRINGE ASSY P60/P80
“4.5 Diff syringe, p 8” 8 XDB034AS SYRINGE, 5DIFF BLOCK+MOTOR ASSY
“4.5 Diff syringe, p 8” 9 XDA826AS MOTOR, BLOCK 5DIFF SYR. P60/P80
“4.5 Diff syringe, p 8” 10 XBA548AS MOTOR, FOR 5 DIFF SYRINGE
“4.5 Diff syringe, p 8” 11 GBG038A SYRINGE, 5DIFF BOTTOM PLATE
“4.5 Diff syringe, p 8” 12 XBA462A CABLE, SYR. MOTORS L=300 P60/P80
“5.Sampling syringe, p 9” 1 GBG044A SYRINGE, SAMP. SYR. BODY P60/P80
“5.Sampling syringe, p 9” 2 FAA064A O’RING, SAMPLING SYR. P60/P80
“5.Sampling syringe, p 9” 3 GBG048A SYRINGE, SAMPLING CROSSPIECE
“5.Sampling syringe, p 9” 4 XDA617AS NEEDLE, 100 µL SAMPL. SYR. P60/P80
“5.Sampling syringe, p 9” 5 XDA593AS SYRINGE, SAMPLING SYRINGE ASSY
“5.Sampling syringe, p 9” 6 XDA834AS SYRINGE, SAMPL BLOCK+MOTOR ASSY
“5.Sampling syringe, p 9” 7 XDA824AS MOTOR, BLOCK SAMPL. SYR.P60/P80
“5.Sampling syringe, p 9” 8 XDA547AS MOTOR, FOR SAMPLING SYRINGE
“5.Sampling syringe, p 9” 9 GBG045A SYRINGE,SAMPLING BOTTOM PLATE
“5.Sampling syringe, p 9” 10 XBA462A CABLE, MOTORS L=300 P60/P80
“6.Reagents syringe, p 10” 1 XDB001AS SYRINGE,REAGENT ASSY P60/P80
“6.Reagents syringe, p 10” 2 GBG033A SYRINGE, REAG BLOCK BODY P60/P80
“6.Reagents syringe, p 10” 3 XDA622A O’RING, REAGENT SYRINGE + WASHER
“6.Reagents syringe, p 10” 4 GBC030A SYRINGE, REAGENT PISTON P60/P80
“6.Reagents syringe, p 10” 5 GBC031A SYRINGE, LYSE PISTON MIC/P60/P80
“6.Reagents syringe, p 10” 6 FAA065A O’RING, REAGENT SYRINGE D=6.3+
“6.Reagents syringe, p 10” 7 XDB005A SYRINGE, REAGENT+MOTOR ASSY P80
Technical Manual
8-2 RAA016CEN
Exploded views
List
N° in
drawing
“6.Reagents syringe, p 10” 8 XDB004AS MOTOR, BLOCK FOR REAG. SYR. P80
“6.Reagents syringe, p 10” 9 XBA390A MOTOR, SYRINGE EQUIPPED P80
“6.Reagents syringe, p 10” 10 GBG034A SYRINGE,5DIFF BLOCK BOTTOM P60
“6.Reagents syringe, p 10” 11 GBG035A SYRINGE,REAGENT HOLDER PLATE
“7.Waste syringe, p 11” 1 XDA827BS SYRINGE, WASTE CPTE P60/C+/P80
“7.Waste syringe, p 11” 2 CAE010A SWITCH, MICROSWITCH XCC5-81
“7.Waste syringe, p 11” 3 FAA017A O'RING, TANK MIN/AG+WASTE MIC
“7.Waste syringe, p 11” 4 GBG219A O'RING, ANTI EXTRUSION RING
“7.Waste syringe, p 11” 5 GBG272A SYRINGE,VACCUM PUMPBODY P60/80
“7.Waste syringe, p 11” 6 FAL009A SILENT BLOCK, FOR SUB ASSEMBLIES
“7.Waste syringe, p 11” 7 XBA458A CABLE,SYR.SWITCH L=300 P60/P80
“7.Waste syringe, p 11” 8 XBA462A CABLE,MOTORS L=300 P60/P80
“7.Waste syringe, p 11” 9 (3+4) XDA621A O’RING, VAC/WASTE PUMP + WASHER
“8.Counting syringe, p 12” 1 XDA828BS SYRINGE, VACUUM CPTE P60/C+/P80
“8.Counting syringe, p 12” 2 CAE010A SWITCH, MICROSWITCH XCC5-81
“8.Counting syringe, p 12” 3 FAA017A O'RING, TANK MIN/AG+WASTE MIC
“8.Counting syringe, p 12” 4 GBG219A O'RING, ANTI EXTRUSION RING
“8.Counting syringe, p 12” 5 GBG273A SYRINGE,VACCUM PUMPBODY P60/80
“8.Counting syringe, p 12” 6 FAL009A SILENT BLOCK, FOR SUB ASSEMBLIES
“8.Counting syringe, p 12” 7 XBA458A CABLE,SYR.SWITCH L=300 P60/P80
“8.Counting syringe, p 12” 8 XBA462A CABLE,MOTORS L=300 P60/P80
“8.Counting syringe, p 12” 9 (3+4) XDA621A O’RING, VAC/WASTE PUMP + WASHER
“9.Diluent tank assy, p 13” 1 XDA626AS CHAMBER, DIL.TANK COVER P60/P80
“9.Diluent tank assy, p 13” 2 GAL094A CHAMBER, WAST.P120/DIL TANK P60
“9.Diluent tank assy, p 13” 3 XEA286AS KIT, O'RING + WASHER P60/P80/P120
“9.Diluent tank assy, p 13” 4 XDA605A CHAMBER, DIL. TANK ASSY P60/P80
“10.Rinse block & needle, p 14” 1 XDA619BS NEEDLE, SAMPLING NEEDLE
“10.Rinse block & needle, p 14” 2 GBG091A CARRIAGE, NEEDLE GUIDE P60
“10.Rinse block & needle, p 14” 3 FAA053A O'RING, SAMPL. NEEDLE
“10.Rinse block & needle, p 14” 4 GBG090A CARRIAGE, RINSING BLOCK P60
“11.Carriage assy, p 15” 1 XDA599AS KIT, CARRIAGE ASSY P60 OT
“11.Carriage assy, p 15” 2 FBR011A BELT, SYRINGE BLOCK 5DIFF/P120
“11.Carriage assy, p 15” 3 XBA396A SENSOR, SAMP NEEDLE MOVE P60/80
“11.Carriage assy, p 15” 4 XBA392A MOTOR, SAMPLING NEEDLE PENTRA60
“11.Carriage assy, p 15” 5 XDA618AS CARRIAGE, NEEDLE CARRIAGE P60
“11.Carriage assy, p 15” 6 DBE014A CABLE, BUSHING D=3,2 BLACK
“11.Carriage assy, p 15” 7 GBG081A NEEDLE, BLOCK SHAFT
“11.Carriage assy, p 15” 8 GBG093A MOTOR, PULLEY P60
“11.Carriage assy, p 15” 9 FBR012A BELT, CARRIAGE L=544 P60
“12.Carriage peripherals, p 16” 1 XBA391B MOTOR, CARRIAGE P60 ACT5D OV&CP
“12.Carriage peripherals, p 16” 2 GBG078A CARRIAGE, HORIZONTAL SHAFT D7.9
“12.Carriage peripherals, p 16” 3 GBG077A CARRIAGE, HORIZONTAL SHAFT D=8
“12.Carriage peripherals, p 16” 4 XBA394A SENSOR, CARRIAGE MOVE PENTRA60
“12.Carriage peripherals, p 16” 5 GBG093A MOTOR, PULLEY P60
Technical Manual
RAA016CEN 8-3
Exploded views
List
N° in
drawing
“13.Optical bench, p 17” 1 DAJ007A OPTICAL, LAMP FOR BENCH P60/P80
“13.Optical bench, p 17” 2 FAL010A AMORTISSEUR HEYMANWELL IBM6047
“13.Optical bench, p 17” 3 XDA601CS CHAMBER, LMNE FLOWCELL P60/P80
“13.Optical bench, p 17” 4 XBA399C FITTING, GROUND FLOWCELL P60/80
“13.Optical bench, p 17” 5 XBA490A CABLE, LMNE FLOWCEL CATHOD WIRE
“13.Optical bench, p 17” 6 XBA403A TUBING, SHIELD REPARTITOR P60
“13.Optical bench, p 17” 7 XEA616BS KIT, FLOWCELL INJECTOR TUBE P60
“13.Optical bench, p 17” 8 XBA193A CABLE, COAX BASO-O.D LMNE-LMNE
“13.Optical bench, p 17” 9 XEA663A CABLE, COAX LMNE FLOWCEL P60/80
“13.Optical bench, p 17” 10 XDA600AS OPTICAL, BENCH COMPLETE P60
“14.Chambers for instrument NON
1 XDB035C 5 CHAMBERS BLOCK ASSY EQUIPED
equipped with Takasago valves, p 18”
2 XDB036BS 4 CHAMBERS BLOCK ASSY EQUIPED
3 XDB019BS WBC/BASO CHAMBER EQUIPED
4 XBA620A SENSOR, CHAMBERS DRAINING P60
“15.Chambers for instrument equipped
1 XDB021E 5 CHAMBERS BLOCK ASSY EQUIPED
with Takasago valves, p 19”
2 XDB017ES 4 CHAMBERS BLOCK ASSY EQUIPED
4 XDB250AS 4 CHAMBERS BLOCK ASSY + COUNTING HEAD
“16.Chamber parts, p 20” 1 XDB021E 5 CHAMBERS BLOCK ASSY
“16.Chamber parts, p 20” 2 GBG326A WBC/BASO CHAMBER NAKED
“16.Chamber parts, p 20” 3 GBG275A FLAT JOINT, COUNTING HEAD
“16.Chamber parts, p 20” 4 FAA066A O’ RING, DRAINING CHAMBER
“16.Chamber parts, p 20” 5 FAK001A APERTURE 50µ
“16.Chamber parts, p 20” 6 GBG003A DRAINING, 1/4 TURN
“16.Chamber parts, p 20” 7 GBG007A CHAMBER, DRAINING CHICANE
“16.Chamber parts, p 20” 8 GBG157A CHAMBER, COUNTING HEAD
“16.Chamber parts, p 20” 9 XBA398B COAXIAL RBC & WBC PENTRA 60 CE
“16.Chamber parts, p 20” 10 FAK003A APERTURE 80µ
HGB PHOTOMETER (HGB PHOTOCELL ALONE:
“16.Chamber parts, p 20” 11 + 11* XBA389A
CAX005A)
“17.Thermostated room, p 21” 1 XDB032BS HEATER, BLOCK COMPLETE P60/ACT
“17.Thermostated room, p 21” 2 XCA166A CHAMBER, ISOLATOR (LONG)
“17.Thermostated room, p 21” 3 GBC015A CLIP, MIX CH. HOLDER MIC/P120
“17.Thermostated room, p 21” 4 GBG013A CUP, OVERFLOW T° ROOM
“17.Thermostated room, p 21” 5 GBG205A PROTECTION, FOR VALVES T°ROOM
“17.Thermostated room, p 21” 6 CAE006A SWITCH, MICROSWITCH XC5-81-82
Technical Manual
8-4 RAA016CEN
Exploded views
List
N° in
drawing
“17.Thermostated room, p 21” 7 XDA812A KIT, T° ROOM FAN + HARNESS P60
“17.Thermostated room, p 21” 8 XEA906AS KIT, INSULATING PADS P60/ACT
“18.Electronic boards, p 22” 1 XAA425C PCB, MAIN BOARD PENTRA60 C+
“18.Electronic boards, p 22” 2 XAA423BS PCB, PREAMP+MECA ASSY PENTRA60
“18.Electronic boards, p 22” 3 XAA427BS PCB, LINK BOARD PENTRA60
“18.Electronic boards, p 22” 4 XAA429AS PCB, LED BOARD FOR COVER P60/80
“19.Straws & tubes, p 23” 1 GBG145A REAGENT, STRAW D=20 P60/P80
“19.Straws & tubes, p 23” 2 GBG155A REAGENT, BOTTLE STOPPER D=25
“19.Straws & tubes, p 23” 3 GBG245A REAGENT, STRAW D=28 P60/P80/ACT
“19.Straws & tubes, p 23” 4 GAK302A REAGENT, BOTTLE STOPPER D=40
“19.Straws & tubes, p 23” 5 XDB119A TUBING, DILUENT ASPIRATION P60
“19.Straws & tubes, p 23” 6 XDA693A TUBING, WASTE P60/CRP200
“19.Straws & tubes, p 23” 7 EAC018A FITTING, LUER MALE/FEMALE I=2,5
“19.Straws & tubes, p 23” 8 XEA018A REAGENT, STRAW DILUENT L=360
“20.Reagent connection assembly, p 24” 1 XDA623AS REAGENT, BLOCK EQUIPED P60/P80
“20.Reagent connection assembly, p 24” 2 EAC008A FITTING, ANTI ROTATION WASHER
“20.Reagent connection assembly, p 24” 3 EAC010A FITTING, LUER FEMALE I=3MM
“21.Miscellaneous, p 24” 1 XBA393A FAN, MAIN FAN 24V P60/P80/P400
“21.Miscellaneous, p 24” 2 XBA509B PCB, BARCODE HAND READER
“22.Power supply, p 25” 1 DBN004C PCB, POWER SUPPLY BLOCK P60
“23.Covers, p 26” 1 XDA644A COVER, LEFT SIDE EQUIPPED P60
“23.Covers, p 26” 2 GBG130A COVER, TOP COVER P60
“23.Covers, p 26” 3 XDA624BS DOOR, FOR T° ROOM EQUIPPED P60
“23.Covers, p 26” 4 XDA628BS COVER, FRONT COVER EQUIPPED P60
“23.Covers, p 26” 5 HAW008A STICKER, REAGENTS NAMES P60
“23.Covers, p 26” 6 HAX0312 STICKER, REAGENT DOOR P60
“23.Covers, p 26” 7 HAX0310 STICKER, SCREEN & KEYBOARD P60
“23.Covers, p 26” 8 FZZ024A DOOR, PUSH PUSH P60
“23.Covers, p 26” 9 XD629AS COVER,REAGENT DOOR EQUIPED P60
Technical Manual
RAA016CEN 8-5
Exploded views
Valves
2. Valves
1 5
6
7
Number Reference Designation

1 XDB123A VALVE, LIQ 11 VALVE ASSY P60 (1 to 11)
4 XDB126A VALVE, LIQ 7 VALVE ASSY P60 (20 to 26
6 XDB135A 2W VALVE WITHOUT COIL
7 XDB136A 3W VALVE WITHOUT COIL
Technical Manual
8-6 RAA016CEN
Exploded views
Takasago valve assembly
3. Takasago valve assembly
3
4

1 XBA685A VALVE, TAKASAGO + MANIFOLD ASSY
2 XBA646AS TAKASAGO VALVE
3 EZZ003A TAKASAGO VALVE MANIFOLD
4 XEA960AS KIT, TAKASAGO KIT O'RING + SCREW
5 XEA959AS TAKASAGO WIRING KIT
Technical Manual
RAA016CEN 8-7
Exploded views
5 Diff syringe
4. 5 Diff syringe
7 1
8
2
3 x4
4 x2
12
10 5
11
6 x2

1 GBG037A SYRINGE, 5DIFF BLOC BODY P60/80
2 FAA040A O'RING, 5DIFF SYRINGE D=12,1
3 FAA067A O'RING, 5DIFF SYRINGE D=2,4
4 GBG042A SYRINGE, CROSSPIECE P60/SPS/P80
5 GBG040A SYRINGE, 5DIFF PISTON P60/P80
6 XDA616AS NEEDLE, 190µL SYR. P60/SPS/P80
7 XDA591AS SYRINGE, 5 DIFF SYRINGE ASSY P60/P80
8 XDB034AS SYRINGE, 5DIFF BLOCK+MOTOR ASSY
9 XDA826AS MOTOR, BLOCK 5DIFF SYR. P60/P80
10 XBA548AS MOTOR, FOR 5 DIFF SYRINGE
11 GBG038A SYRINGE, 5DIFF BOTTOM PLATE
12 XBA462A CABLE, SYR. MOTORS L=300 P60/P80
Technical Manual
8-8 RAA016CEN
Exploded views
Sampling syringe
5. Sampling syringe
2 x2
8
4
10

1 GBG044A SYRINGE, SAMP. SYR. BODY P60/P80
2 FAA064A O’RING, SAMPLING SYR. P60/P80
3 GBG048A SYRINGE, SAMPLING CROSSPIECE
4 XDA617AS NEEDLE, 100 µL SAMPL. SYR. P60/P80
5 XDA593AS SYRINGE, SAMPLING SYRINGE ASSY
6 XDA834AS SYRINGE, SAMPL BLOCK+MOTOR ASSY
7 XDA824AS MOTOR, BLOCK SAMPL. SYR.P60/P80
8 XDA547AS MOTOR, FOR SAMPLING SYRINGE
9 GBG045A SYRINGE,SAMPLING BOTTOM PLATE
10 XBA462A CABLE, MOTORS L=300 P60/P80
Technical Manual
RAA016CEN 8-9
Exploded views
Reagents syringe
6. Reagents syringe
2
11
3
x4
6
4
8 x4
7 5
10

1 XDB001AS SYRINGE,REAGENT ASSY P60/P80
2 GBG033A SYRINGE, REAG BLOCK BODY P60/P80
3 XDA622A O’RING, REAGENT SYRINGE + WASHER
4 GBC030A SYRINGE, REAGENT PISTON P60/P80
5 GBC031A SYRINGE, LYSE PISTON MIC/P60/P80
6 FAA065A O’RING, REAGENT SYRINGE D=6.3+
7 XDB005A SYRINGE, REAGENT+MOTOR ASSY P80
8 XDB004AS MOTOR, BLOCK FOR REAG. SYR. P80
9 XBA390A MOTOR, SYRINGE EQUIPPED P80
10 GBG034A SYRINGE,5DIFF BLOCK BOTTOM P60
11 GBG035A SYRINGE,REAGENT HOLDER PLATE
Technical Manual
8 - 10 RAA016CEN
Exploded views
Waste syringe
7. Waste syringe
2 9
3
4
6 x4

1 XDA827BS SYRINGE, WASTE CPTE P60/C+/P80
2 CAE010A SWITCH, MICROSWITCH XCC5-81
3 FAA017A O'RING, TANK MIN/AG+WASTE MIC
4 GBG219A O'RING, ANTI EXTRUSION RING
5 GBG272A SYRINGE,VACCUM PUMPBODY P60/80
6 FAL009A SILENT BLOCK, FOR SUB ASSEMBLIES
7 XBA458A CABLE,SYR.SWITCH L=300 P60/P80
8 XBA462A CABLE,MOTORS L=300 P60/P80
9 (3+4) XDA621A O’RING, VAC/WASTE PUMP + WASHER
Technical Manual
RAA016CEN 8 - 11
Exploded views
Counting syringe
8. Counting syringe
2 9
3
4
6 x4

1 XDA828BS SYRINGE, VACUUM CPTE P60/C+/P80
2 CAE010A SWITCH, MICROSWITCH XCC5-81
3 FAA017A O'RING, TANK MIN/AG+WASTE MIC
4 GBG219A O'RING, ANTI EXTRUSION RING
5 GBG273A SYRINGE,VACCUM PUMPBODY P60/80
6 FAL009A SILENT BLOCK, FOR SUB ASSEMBLIES
7 XBA458A CABLE,SYR.SWITCH L=300 P60/P80
8 XBA462A CABLE,MOTORS L=300 P60/P80
9 (3+4) XDA621A O’RING, VAC/WASTE PUMP + WASHER
Technical Manual
8 - 12 RAA016CEN
Exploded views
Diluent tank assy
9. Diluent tank assy

1 XDA626AS CHAMBER, DIL.TANK COVER P60/P80
2 GAL094A CHAMBER, WAST.P120/DIL TANK P60
3 XEA286AS KIT, O'RING + WASHER P60/P80/P120
4 XDA605A CHAMBER, DIL. TANK ASSY P60/P80
Technical Manual
RAA016CEN 8 - 13
Exploded views
Rinse block & needle
10. Rinse block & needle
1 2 3 4

1 XDA619BS NEEDLE, SAMPLING NEEDLE
2 GBG091A CARRIAGE, NEEDLE GUIDE P60
3 FAA053A O'RING, SAMPL. NEEDLE
4 GBG090A CARRIAGE, RINSING BLOCK P60
Technical Manual
8 - 14 RAA016CEN
Exploded views
Carriage assy
11. Carriage assy
1
2
6
9
7
8

1 XDA599AS KIT, CARRIAGE ASSY P60 OT
2 FBR011A BELT, SYRINGE BLOCK 5DIFF/P120
3 XBA396A SENSOR, SAMP NEEDLE MOVE P60/80
4 XBA392A MOTOR, SAMPLING NEEDLE PENTRA60
5 XDA618AS CARRIAGE, NEEDLE CARRIAGE P60
6 DBE014A CABLE, BUSHING D=3,2 BLACK
7 GBG081A NEEDLE, BLOCK SHAFT
8 GBG093A MOTOR, PULLEY P60
9 FBR012A BELT, CARRIAGE L=544 P60
Technical Manual
RAA016CEN 8 - 15
Exploded views
Carriage peripherals
12. Carriage peripherals
4 5

1 XBA391B MOTOR, CARRIAGE P60 ACT5D OV&CP
2 GBG078A CARRIAGE, HORIZONTAL SHAFT D7.9
3 GBG077A CARRIAGE, HORIZONTAL SHAFT D=8
4 XBA394A SENSOR, CARRIAGE MOVE PENTRA60
5 GBG093A MOTOR, PULLEY P60
Technical Manual
8 - 16 RAA016CEN
Exploded views
Optical bench
13. Optical bench
10
2 x4
7
3
4
8
6
5

1 DAJ007A OPTICAL, LAMP FOR BENCH P60/P80
2 FAL010A AMORTISSEUR HEYMANWELL IBM6047
3 XDA601CS CHAMBER, LMNE FLOWCELL P60/P80
4 XBA399C FITTING, GROUND FLOWCELL P60/80
5 XBA490A CABLE, LMNE FLOWCEL CATHOD WIRE
6 XBA403A TUBING, SHIELD REPARTITOR P60
7 XEA616BS KIT, FLOWCELL INJECTOR TUBE P60
8 XBA193A CABLE, COAX BASO-O.D LMNE-LMNE
9 XEA663A CABLE, COAX LMNE FLOWCEL P60/80
10 XDA600AS OPTICAL, BENCH COMPLETE P60
Technical Manual
RAA016CEN 8 - 17
Exploded views
Chambers for instrument NON equipped with Takasago valves
14. Chambers for instrument NON equipped with

Takasago valves
1 2

1 XDB035C 5 CHAMBERS BLOCK ASSY EQUIPED
2 XDB036BS 4 CHAMBERS BLOCK ASSY EQUIPED
Technical Manual
8 - 18 RAA016CEN
Exploded views
Chambers for instrument equipped with Takasago valves
15. Chambers for instrument equipped with

Takasago valves
1 2

1 XDB021E 5 CHAMBERS BLOCK ASSY EQUIPED
2 XDB017ES 4 CHAMBERS BLOCK ASSY EQUIPED
4 XDB250AS 4 CHAMBERS BLOCK ASSY + COUNTING HEAD
Technical Manual
RAA016CEN 8 - 19
Exploded views
Chamber parts
16. Chamber parts
9 x2
2
3 x4
11
8 x2
10
4
x4
7
x4
1 6 x4 11*

1 XDB021E 5 CHAMBERS BLOCK ASSY
2 GBG326A WBC/BASO CHAMBER NAKED
3 GBG275A FLAT JOINT, COUNTING HEAD
4 FAA066A O’ RING, DRAINING CHAMBER
5 FAK001A APERTURE 50µ
6 GBG003A DRAINING, 1/4 TURN
7 GBG007A CHAMBER, DRAINING CHICANE
8 GBG157A CHAMBER, COUNTING HEAD
9 XBA398B COAXIAL RBC & WBC PENTRA 60 CE
10 FAK003A APERTURE 80µ
11 + 11* XBA389A HGB PHOTOMETER (HGB PHOTOCELL ALONE: CAX005A)
Technical Manual
8 - 20 RAA016CEN
Exploded views
Thermostated room
17. Thermostated room
1
8 3
5 4

1 XDB032BS HEATER, BLOCK COMPLETE P60/ACT
2 XCA166A CHAMBER, ISOLATOR (LONG)
3 GBC015A CLIP, MIX CH. HOLDER MIC/P120
4 GBG013A CUP, OVERFLOW T° ROOM
5 GBG205A PROTECTION, FOR VALVES T°ROOM
6 CAE006A SWITCH, MICROSWITCH XC5-81-82
7 XDA812A KIT, T° ROOM FAN + HARNESS P60
8 XEA906AS KIT, INSULATING PADS P60/ACT
Technical Manual
RAA016CEN 8 - 21
Exploded views
Electronic boards
18. Electronic boards
4
1

1 XAA425C PCB, MAIN BOARD PENTRA60
2 XAA423BS PCB, PREAMP+MECA ASSY PENTRA60
3 XAA427BS PCB, LINK BOARD PENTRA60
4 XAA429AS PCB, LED BOARD FOR COVER P60/80
Technical Manual
8 - 22 RAA016CEN
Exploded views
Straws & tubes
19. Straws & tubes
3
2
5
6

1 GBG145A REAGENT, STRAW D=20 P60/P80
2 GBG155A REAGENT, BOTTLE STOPPER D=25
3 GBG245A REAGENT, STRAW D=28 P60/P80/ACT
4 GAK302A REAGENT, BOTTLE STOPPER D=40
5 XDB199A TUBING, DILUENT ASPIRATION P60
6 XDA693A TUBING, WASTE P60/CRP200
7 EAC018A FITTING, LUER MALE/FEMALE I=2,5
8 XEA018A REAGENT, STRAW DILUENT L=360
Technical Manual
RAA016CEN 8 - 23
Exploded views
Reagent connection assembly
20. Reagent connection assembly
2
x2
3
1
x2

1 XDA623AS REAGENT, BLOCK EQUIPED P60/P80
2 EAC008A FITTING, ANTI ROTATION WASHER
3 EAC010A FITTING, LUER FEMALE I=3MM
21. Miscellaneous
1
2

1 XBA393A FAN, MAIN FAN 24V P60/P80/P400
2 XBA509B PCB, BARCODE HAND READER
Technical Manual
8 - 24 RAA016CEN
Exploded views
Power supply
22. Power supply

1 DBN004C PCB, POWER SUPPLY BLOCK P60
Technical Manual
RAA016CEN 8 - 25
Exploded views
Covers
23. Covers
3 1
5 4
9 8

1 XDA644A COVER, LEFT SIDE EQUIPPED P60
2 GBG130A COVER, TOP COVER P60
3 XDA624BS DOOR, FOR T° ROOM EQUIPPED P60
4 XDA628BS COVER, FRONT COVER EQUIPPED P60
5 HAW008A STICKER, REAGENTS NAMES P60
6 HAX0312 STICKER, REAGENT DOOR P60
7 HAX0310 STICKER, SCREEN & KEYBOARD P60
8 FZZ024A DOOR, PUSH PUSH P60
9 XD629AS COVER,REAGENT DOOR EQUIPED P60
Technical Manual
8 - 26 RAA016CEN

Manual Técnico PENTRA 60 (2020 - 08 - 20 02 - 50 - 44 UTC)

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Technical manual

HORIBA ABX SAS

2. Legal information ..................................................................................................3

3. Operational conditions .........................................................................................5

4. Environmental protection .....................................................................................7

2 - Hydraulic & pneumatic principles

1. Hydropneumatic connections ..............................................................................2

2. Function of valves .................................................................................................8

3. Pneumatic diagrams .............................................................................................9

3 - Electric & electronic principles

1. Main board test points ..........................................................................................2

3. Flat cable list .........................................................................................................8

4 - Analysis cycle technology

1. Analysis cycle description ....................................................................................2

2. Measuring principles ............................................................................................8

3. Raw counts ..........................................................................................................17

2. USER menu overview ...........................................................................................3

3. MAINTENANCE menu overview ...........................................................................4

4. SETUP menu overview .........................................................................................5

5. TECHNICIAN menu overview ...............................................................................6

6 - Alarm & error list

4. Technical Operations ............................................................................................3

RAS236C: 6 month maintenance

RAS237C: Yearly maintenance

RAS238C: Every 2 years maintenance

RAS239C: Decontamination & rinse

RAS240C: LMNE flowcell adjustment

RAS241C: Chambers adjustment

RAS242C: Needle adjustment

RAS243C: Main board adjustment

RAS244C: Temperature adjustment

RAS245C: Vacuum adjustment

RAS247C: Covers dismantling

RAS248C: Heater assy replacement

RAS249C: Bubbling adjustment

RAS250C: Power supply replacement

RAS251C: Start cycle microswitch replacement

RAS252C: Check up after intervention

RAS253C: Step by step analysis cycle

RAS254C: Optical bench dismantling & replacement

RAS255C: External barcode reader configuration

RAS304C: LMNE balance adjustment

RAS680A: Takasago valve assembly dismantling

3. Takasago valve assembly ....................................................................................7

4. 5 Diff syringe ..........................................................................................................8

5. Sampling syringe ...................................................................................................9

6. Reagents syringe ................................................................................................10

7. Waste syringe ......................................................................................................11

8. Counting syringe .................................................................................................12

9. Diluent tank assy .................................................................................................13

10. Rinse block & needle ........................................................................................14

11. Carriage assy .....................................................................................................15

12. Carriage peripherals .........................................................................................16

13. Optical bench ....................................................................................................17

15. Chambers for instrument equipped with Takasago valves ..........................19

16. Chamber parts ...................................................................................................20

17. Thermostated room ..........................................................................................21

18. Electronic boards ..............................................................................................22