Composition of EuroFlow Panels and Technical Information On Reagents Version 1.4

Composition of EuroFlow panels and technical information on reagents

Version 1.4 (11 February 2016) (Updates in blue boxes outlined in red)

Published as Appendix in:
Van Dongen JJM, Lhermitte L, Böttcher S, Almeida J, van der Velden VHJ, Flores‐Montero J,
Rawstron A, Asnafi V, Lécrevisse Q, Lucio P, Mejstrikova E, Szczepański T, Kalina T, de Tute R,
Brüggemann M, Sedek L, Cullen M, Langerak AW, Mendonça A, Macintyre E, Martin‐Ayuso M,
Hrusak O, Vidriales MB, Orfao A. EuroFlow antibody panels for standardized n‐dimensional flow
cytometric immunophenotyping of normal, reactive and malignant leukocytes. Leukemia 2012;
26: 1908‐1975.

Disclaimer on EuroFlow reference reagents and equivalent reagents
EuroFlow reference reagents, obtained from multiple different companies, have been tested under specific
conditions for usage at specific concentrations in the EuroFlow antibody combinations as reported in the EuroFlow
documents. The recommended reagents aim to have comparable results in the (inter‐laboratory) standardized
EuroFlow staining procedures. However, EuroFlow cannot be responsible for any change in the manufacturing of
the reagents, such as concentration, purity, dye/protein ratio, or any other factor that may affect the performance
of these products both as single reagents and in the combinations in which they are included.
The so‐called “equivalent reagents” have been tested as single reagents in comparison with the EuroFlow
reference reagents. Performance of equivalent reagents in each of the individual EuroFlow 8‐color combinations or
modifications in the performance characteristics of equivalent reagents (e.g. in different lots) is out of the
EuroFlow control.
Page 1 of 14

Updated: 11 February 2016 EuroFlow antibody panels version 1.4

Updated in Version 1.4
 Catalogue number CD20‐PacB present in LST, SST, BCP‐ALL, B‐CLPD
 Catalogue number CD38‐APCH7 present in ALOT, LST, SST, PCD, BCP‐ALL, T‐
ALL, AML, B‐CLPD, T‐CLPD, NK‐CLPD
 Catalogue number and titer TCR‐PECy7 present in LST and B‐CLPD
 Catalogue number and titer CD27‐PerCPCy5.5 present in PCD
 Catalogue number CD81‐APCH7 present in PCD, BCP‐ALL and B‐CLPD
 Catalogue number and titer 2micro‐PerCPCy5.5 present in PCD
 Catalogue number and titer CD24‐APCH7 present in BCP‐ALL
 Catalogue number and titer CD9‐APCH7 present in AML/MDS
 Catalogue number CD10‐APCH7 present in AML/MDS panel
 Company, Catalogue number and titer CD64‐PE present in AML/MDS panel
 Catalogue number and titer CD71‐APCH7 present in AML/MDS panel
 Clone, Company, Catalogue number and titer CD105‐PE present in AML/MDS
panel
 Catalogue number and titer CD11C‐PerCPCy5.5 present in B‐CLPD panel
 Catalogue number and titer CD22‐PerCPCy5.5 present in B‐CLPD panel
 Catalogue number and titer CD43‐APCH7 present in B‐CLPD panel
 Catalogue number and titer CD79b‐PerCPCy5.5 present in B‐CLPD panel
 Catalogue number and titer CD49d‐APCH7 present in B‐CLPD panel
 European catalogue number of CD117‐PECy7 present in AML/MDS panel
 Titer CD34‐PerCPCy5.5 present in ALOT, BCP‐ALL and AML

Page 2 of 14


Panel 1. Composition of ALOT and technical information on reagents
PacB PacO FITC PE PerCPCy5.5 PECy7 APC APCH7

CyCD3 CD45 CyMPO CyCD79a CD34 CD19 CD7 SmCD3

Catalogue
Marker Fluorochrome Clone Source Application in EuroFlow panel l/test
number
CyCD3 PacB UCHT1 BD Biosciences 558117 ALOT, T‐ALL 7
SmCD3 APCH7 SK7 BD Biosciences 641415 ALOT, T‐ALL 3
CD7 APC 124‐1D1 eBioscience 17‐0079‐42 ALOT, T‐ALL, AML 2
CD19 PECy7 J3‐119 Beckman Coulter IM3628 ALOT, LST, SST, BCP‐ALL, PCD 5
CD34 PerCPCy5.5 8G12 BD Biosciences 347222 ALOT, BCP‐ALL, AML 10
CD45 PacO HI30 Invitrogen MHCD4530 ALOT, LST, SST, BCP‐ALL, T‐ALL, 5
AML, B‐CLPD, T‐CLPD, NK‐CLPD
CyCD79a PE HM57 Dako R7159 ALOT 5
CyMPO FITC MPO‐7 Dako F0714 ALOT 3

Page 3 of 14


Panel 2. Composition of LST and technical information on reagents

CD20 CD8 and CD56 and CD19 and
CD45 CD5 SmCD3 CD38
and CD4 SmIg SmIg TCR

Catalogue
number
SmCD3 APC SK7 BD Biosciences 345767 LST, SST, B‐CLPD 2.5
CD4 PacB RPA‐T4 BioLegend 300521 LST, B‐CLPD, T‐CLPD 0.5
CD5 PerCPCy5.5 L17F12 BD Biosciences 341109 LST, T‐ALL, B‐CLPD 15
CD8 FITC UCH‐T4 Cytognos CYT‐SLPC‐50 LST, B‐CLPD 20 (Part of LST
mixture)
CD19 PECy7 J3‐119 Beckman Coulter IM3628 LST, ALOT, SST, BCP‐ALL, PCD 5
CD20 PacB 2H7 BioLegend 302320 LST, SST, BCP‐ALL, B‐CLPD 1

CD38 APCH7 HB7 BD Biosciences EU: 656646 LST, SST, BCP‐ALL, T‐ALL, AML, B‐ 3
US: 653314 CLPD
CD45 PacO HI30 Invitrogen MHCD4530 LST, ALOT, SST, BCP‐ALL, T‐ALL, 5
CD56 PE C5.9 Cytognos CYT‐SLPC‐50 LST, B‐CLPD 20 (Part of LST
mixture)
SmIg PE polyclonal Cytognos CYT‐SLPC‐50 LST, B‐CLPD 20 (Part of LST
mixture)
SmIg FITC polyclonal Cytognos CYT‐SLPC‐50 LST, B‐CLPD 20 (Part of LST
mixture)
EU: 655410
TCR PECy7 11F2 BD Biosciences LST, B‐CLPD 3
US: 655434

Page 4 of 14


Panel 3. Composition of SST and technical information on reagents

CD8 and CD56 and SmCD3 and
CD20 CD45 CD4 CD19 CD38
SmIg SmIg CD14

Catalogue
number
SmCD3 APC SK7 BD Biosciences 345767 SST, LST, B‐CLPD 2.5
CD4 PerCPCy5.5 SK3 Cytognos CYT‐SLPC4‐50 SST 20 (Part of SST
mixture)
CD8 FITC UCH‐T4 Cytognos CYT‐SLPC4‐50 SST 20 (Part of SST
mixture)
CD14 APC MP9 BD Biosciences 345787 SST 5
CD19 PECy7 J3‐119 Beckman Coulter IM3628 SST, ALOT, LST, BCP‐ALL, PCD 5
CD20 PacB 2H7 BioLegend 302320 SST, LST, BCP‐ALL, B‐CLPD 1

CD38 APCH7 HB7 BD Biosciences EU: 656646 3
SST, LST, BCP‐ALL, AML, B‐CLPD
US: 653314
CD45 PacO HI30 Invitrogen MHCD4530 SST, ALOT, LST, BCP‐ALL, T‐ALL, 5
CD56 PE C5.9 Cytognos CYT‐SLPC4‐50 SST 20 (Part of SST
mixture)
SmIg PE polyclonal Cytognos CYT‐SLPC4‐50 SST 20 (Part of SST
mixture)
SmIg FITC polyclonal Cytognos CYT‐SLPC4‐50 SST 20 (Part of SST
mixture)

Page 5 of 14


Panel 4. Composition of PCD and technical information on reagents
Tube PacB PacO FITC PE PerCPCy5.5 PECy7 APC APCC750
1 CD45 CD138 CD38 CD56 2micro CD19 CyIg CyIg

 APCH7
2 CD45 CD138 CD38 CD28 CD27 CD19 CD117 CD81

Catalogue
number
CD19 PECy7 J3‐119 Beckman Coulter IM3628 PCD, ALOT, LST, SST, BCP‐ALL 5
CD27 PerCPCy5.5 L128 BD Biosciences EU: 656643 PCD
20
US: 655429
CD28 PE L293 BD Biosciences 348047 PCD 20
CD38 FITC LD38 Cytognos CYT‐38F PCD 3*
CD38 pure LD38 Cytognos CYT‐38P1 PCD 2* } 5*
CD45 PacB T29/33 Dako PB986 PCD 5

CD56 PE C5.9 Cytognos CYT‐56PE PCD, AML 5
CD81 APCH7 JS‐81 BD Biosciences EU: 656647 PCD, BCP‐ALL, B‐CLPD 5
US: 656154
CD117 APC 104D2 BD Biosciences 333233 PCD, BCP‐ALL 5
CD138 PacO B‐A38 Exbio PO‐520 PCD 4
2micro PerCPCy5.5 Tü99 BD Biosciences EU: 656645 PCD
15#
US: 655435
2micro pure Tü99 BD Biosciences 555550 PCD
CyIg APC polyclonal Dako C0222 PCD 2.5
rabbit serum
CyIg APCC750 polyclonal Cytognos CYT‐LAC750 PCD, BCP‐ALL 5
* Mixture of fluorochrome‐conjugated and –unconjugated antibodies is used to reduce the signal intensity, while retaining saturation
conditions to avoid unpredictable variation in staining patterns.
# The antibody concentration of the conjugated 2microglobulin antibody has changed in such a way that it is not anymore needed to
use the antibody in a mixture with the unconjugated antibody as used previously.

Page 6 of 14


Panel 5. Composition of BCP‐ALL and technical information on reagents
Tube PacB PacO FITC PE PerCPCy5.5 PECy7 APC APCH7

1 CD20 CD45 CD58 CD66c CD34 CD19 CD10 CD38
APCC750
SmIgM and
2 SmIg CD45 CyIg CD33 CD34 CD19 SmIg
CD117
APCH7
3 CD9 CD45 NuTdT CD13 CD34 CD19 CD22 CD24
CD15 and
4 CD21 CD45 NG2 CD34 CD19 CD123 CD81
CD65

Catalogue
number
CD9 PacB MEM‐61 Exbio PB‐208‐T100 BCP‐ALL 4
CD10 APC HI10A BD Biosciences 332777 BCP‐ALL 5
CD13 PE L138 BD Biosciences 347406 BCP‐ALL, T‐ALL, AML 7
CD15 FITC MMA BD Biosciences 332778 BCP‐ALL, AML 10
CD19 PECy7 J3‐119 Beckman Coulter IM3628 BCP‐ALL, ALOT, LST, SST, PCD 5
CD20 PacB 2H7 BioLegend 302320 BCP‐ALL, LST, SST, B‐CLPD 1
CD21 PacB LT21 Exbio PB‐306‐T100 BCP‐ALL 4
CD22 APC S‐HCL‐1 BD Biosciences 333145 BCP‐ALL 5
CD24 APCH7 ML5 BD Biosciences EU: 658331 BCP‐ALL 5
CD33 PE P67.6 BD Biosciences 345799 BCP‐ALL 5
CD34 PerCPCy5.5 8G12 BD Biosciences 347222 BCP‐ALL, ALOT, AML 10
CD38 APCH7 HB7 BD Biosciences EU: 656646 BCP‐ALL, LST, SST, AML, B‐CLPD 3
US: 653314
CD45 PacO HI30 Invitrogen MHCD4530 BCP‐ALL, ALOT, LST, SST, T‐ALL, AML, T‐ 5
CLPD, NK‐CLPD
CD58 FITC 1C3 BD Biosciences 555920 BCP‐ALL 7
CD65 FITC 88H7 Beckman Coulter IM1654U BCP‐ALL 7
CD66c PE KOR‐SA3544 Beckman Coulter IM2357U BCP‐ALL 10
CD81 APCH7 JS‐81 BD Biosciences EU: 655410 BCP‐ALL, PCD, B‐CLPD 5
US: 655434
CD117 APC 104D2 BD Biosciences 333233 BCP‐ALL, PCD 5
CD123 APC AC145 Miltenyi Biotec 130‐090‐901 BCP‐ALL, T‐ALL 7
SmIg PacB A8B5 Exbio PB‐504‐T100 BCP‐ALL 4
SmIg APCC750 polyclonal Cytognos CYT‐LAC750 BCP‐ALL, PCD 5
CyIg FITC polyclonal Dako F0058 BCP‐ALL 10
rabbit serum
SmIgM APC G20‐127 BD Biosciences 551062 BCP‐ALL, B‐CLPD 10
NG2 PE 7.1 Beckman Coulter IM3454U BCP‐ALL, AML 10
NuTdT FITC HT‐6 Dako F7139 BCP‐ALL, T‐ALL, AML 10

Page 7 of 14


Panel 6. Composition of T‐ALL and technical information on reagents
1 CyCD3 CD45 NuTdT CD99 CD5 CD10 CD1a SmCD3

2 CyCD3 CD45 CD2 CD117 CD4 CD8 CD7 SmCD3
3 CyCD3 CD45 TCR TCR CD33 CD56 CyTCR SmCD3
4 CyCD3 CD45 CD44 CD13 HLADR CD45RA CD123 SmCD3

Catalogue
number
CD1a APC HI149 BD Biosciences 559775 T‐ALL 5
CD2 FITC RPA‐2.10 BD Biosciences 555326 T‐ALL 5
CyCD3 PacB UCHT1 BD Biosciences 558117 T‐ALL, ALOT 7
SmCD3 APCH7 SK7 BD Biosciences 641415 T‐ALL, ALOT 3
CD4 PerCPCy5.5 SK3 BD Biosciences 332772 T‐ALL 7
CD5 PerCPCy5.5 L17F12 BD Biosciences 341109 T‐ALL, LST, B‐CLPD 15
CD7 APC 124‐1D1 eBioscience 17‐0079‐42 T‐ALL, ALOT, AML 2
CD8 PECy7 SFCI21Thy2D3 Beckman Coulter 737661 T‐ALL 5
CD10 PECy7 HI10A BD Biosciences 341112 T‐ALL 5
CD13 PE L138 BD Biosciences 347406 T‐ALL, BCP‐ALL, AML 7
CD33 PerCPCy5.5 P67.6 BD Biosciences 333146 T‐ALL 10
CD44 FITC L178 BD Biosciences 347943 T‐ALL 7
CD45 PacO HI30 Invitrogen MHCD4530 T‐ALL, ALOT, LST, SST, BCP‐ALL, AML, T‐ 5
CLPD, NK‐CLPD
CD45RA PECy7 L48 BD Biosciences 337186 T‐ALL 5
CD56 PECy7 N901 Beckman Coulter A21692 T‐ALL, NK‐CLPD 5
CD99 PE Tü12 BD Biosciences 555689 T‐ALL 5
CD117 PE 104D2 BD Biosciences 332785 T‐ALL 5
CD123 APC AC145 Miltenyi Biotec 130‐090‐901 T‐ALL, BCP‐ALL 7
HLA‐DR PerCPCy5.5 L243 BD Biosciences 552764 T‐ALL, B‐CLPD 10
TCR PE IP26A Beckman Coulter A39499 T‐ALL 7
CyTCR APC 8A3 (F1) Cytognos CYT‐BF1AP T‐ALL 3
TCR FITC IMMU510 Beckman Coulter IM1571U T‐ALL 10
NuTdT FITC HT‐6 Dako F7139 T‐ALL, BCP‐ALL, AML 10

Page 8 of 14


Panel 7. Composition of AML/MDS and technical information on reagents

1 HLADR CD45 CD16 CD13 CD34 CD117 CD11b CD10
2 HLADR CD45 CD35 CD64 CD34 CD117 CD300e CD14
3 HLADR CD45 CD36 CD105 CD34 CD117 CD33 CD71
4 HLADR CD45 NuTdT CD56 CD34 CD117 CD7 CD19
5 HLADR CD45 CD15 NG2 CD34 CD117 CD22 CD38
CD42a and
6 HLADR CD45 CD203c CD34 CD117 CD123 CD4
CD61
7 HLADR CD45 CD41 CD25 CD34 CD117 CD42b CD9

Catalogue
number
CD4 APCH7 SK3 BD Biosciences 641398 AML 5
CD7 APC 124‐1D1 eBioscience 17‐0079‐42 AML, ALOT, T‐ALL 2
CD9 APCH7 M‐L13 BD Biosciences EU: 655409 AML 10
CD10 APCH7 HI10A BD Biosciences EU: 655404 AML
5
US: 655426
CD11b APC D12 BD Biosciences 333143 AML 5
CD13 PE L138 BD Biosciences 347406 AML, BCP‐ALL, T‐ALL 7
CD14 APCH7 MP9 BD Biosciences 641394 AML 5
CD15 FITC MMA BD Biosciences 332778 AML, BCP‐ALL 10
CD16 FITC CLB Fc gran/1, 5D2 Sanquin M1604 AML 20
CD19 APCH7 SJ25C1 BD Biosciences 641395 AML, NK‐CLPD 5
CD22 APC S‐HCL‐1 BD Biosciences 333145 AML, BCP‐ALL 5
CD25 PE 2A3 BD Biosciences 341011 AML, T‐CLPD, NK‐CLPD 10
CD33 APC P67.6 BD Biosciences 345800 AML 10
CD34 PerCPCy5.5 8G12 BD Biosciences 347222 AML, ALOT, BCP‐ALL 10
CD35 FITC E11 BD Biosciences 555452 AML 5
CD36 FITC CLB‐IVC7 Sanquin M1613 AML 5
CD38 APCH7 HB7 BD Biosciences EU: 656646 AML, LST, SST, BCP‐ALL, B‐CLPD 3
US: 653314
CD41 FITC CLB‐tromb/7, 6C9 Sanquin M1674 AML 1
CD42a FITC GRP‐P Serotec MCA1227F AML 1
CD42b APC HIP1 BD Biosciences 551061 AML 1
CD45 PacO HI30 Invitrogen MHCD4530 AML, ALOT, LST, SST, BCP‐ALL, T‐ALL, 5
B‐CLPD, T‐CLPD,NK‐CLPD
CD56 PE C5.9 Cytognos CYT‐56PE AML, PCD 5
CD61 FITC RUU‐PL7F12 BD Biosciences 347407 AML 4
CD64 PE 10.1 BD Biosciences 644385 AML 20
CD71 APCH7 M‐A712 BD Biosciences EU: 655408 AML
2
US: 655431
CD105 PE 266 BD Biosciences 560839 AML 5
CD117 PECy7 104D2D1 Beckman Coulter EU: B49221 AML 5
IM3698
CD123 APC AC145 Miltenyi Biotec 130‐090‐901 AML, BCP‐ALL, T‐ALL 10
CD203c PE 97A6 Beckman Coulter IM3575 AML 10
CD300e APC UP‐H2 Immunostep IREM2A‐T100 AML 5
Page 9 of 14


HLADR PacB L243 BioLegend 307624 AML, NK‐CLPD 1 (1:5
dilution)
NG2 PE 7.1 Beckman Coulter IM3454U AML, BCP‐ALL 10
NuTdT FITC HT‐6 Dako F7139 AML, BCP‐ALL, T‐ALL 10

Page 10 of 14


Panel 8. Composition of B‐CLPD and technical information on reagents

CD20 CD8 and CD56 and CD19 and
1 CD45 CD5 SmCD3 CD38
and CD4 SmIg SmIg TCR
2 CD20 CD45 CD23 CD10 CD79b CD19 CD200 CD43
3 CD20 CD45 CD31 CD305 CD11c CD19 SmIgM CD81
4 CD20 CD45 CD103 CD95 CD22 CD19 CD185 CD49d
5 CD20 CD45 CD62L CD39 HLADR CD19 CD27

Catalogue
number
SmCD3 APC SK7 BD Biosciences 345767 B‐CLPD, SST, LST 2.5
CD4 PacB RPA‐T4 BioLegend 300521 B‐CLPD, LST, T‐CLPD 0.5
CD5 PerCPCy5.5 L17F12 BD Biosciences 341109 B‐CLPD, LST, T‐ALL 15
CD8 FITC UCH‐T4 Cytognos CYT‐SLPC‐50 B‐CLPD, LST 20 (part of LST
mixture)
CD10 PE ALB1 Beckman Coulter A07760 B‐CLPD 20
CD11c PerCPCy5.5 B‐Ly6 BD Biosciences 658330 B‐CLPD 10
CD19 PECy7 J3‐119 Beckman Coulter IM3628 B‐CLPD, ALOT, LST, SST, BCP‐ALL, PCD 5
CD20 PacB 2H7 BioLegend 302320 B‐CLPD, LST, SST, BCP‐ALL 1
CD22 PerCPCy5.5 S‐HCL‐1 BD Biosciences 658329 B‐CLPD 25
CD23 FITC MHM6 Dako F7062 B‐CLPD 2.5
CD27 APC L128 BD Biosciences 337169 B‐CLPD 2.5
CD31 FITC WM59 BD Biosciences 555445 B‐CLPD 10
CD38 APCH7 HB7 BD Biosciences EU: 656646 B‐CLPD, LST, SST, BCP‐ALL, AML 3
US: 653314
CD39 PE TÜ66 BD Biosciences 555464 B‐CLPD 10
CD43 APCH7 IG10 BD Biosciences EU: 655407 B‐CLPD
2.5
US: 655430
CD45 PacO HI30 Invitrogen MHCD4530 B‐CLPD, ALOT, LST, SST, BCP‐ALL, T‐ 5
ALL, AML, T‐CLPD, NK‐CLPD)
CD49d APCH7 9F10 BD Biosciences EU: 658332 B‐CLPD 1
CD56 PE C5.9 Cytognos CYT‐SLPC‐50 B‐CLPD, LST 20 (part of LST
mixture)
CD62L FITC SK11 BD Biosciences 347443 B‐CLPD 2.5
CD79b PerCPCy5.5 SN8 BD Biosciences 656644 B‐CLPD 10
CD81 APCH7 JS‐81 BD Biosciences EU: 655410 B‐CLPD, PCD, BCP‐ALL 5
US: 655434
CD95 PE DX2 BD Biosciences 555674 B‐CLPD 20
CD103 FITC Ber‐ACT8 BD Biosciences 333155 B‐CLPD 2
CD185 APC 51505 R&D Systems FAB190A B‐CLPD 10
CD200 APC OX104 eBioscience 17‐9200 B‐CLPD 1.25
CD305 PE DX26 BD Biosciences 550811 B‐CLPD 10
HLA‐DR PerCPCy5.5 L243 BD Biosciences 552764 B‐CLPD, T‐ALL 10
SmIgk PE polyclonal Cytognos CYT‐SLPC‐50 B‐CLPD, LST 20 (part of LST
mixture)
SmIg FITC polyclonal Cytognos CYT‐SLPC‐50 B‐CLPD, LST 20 (part of LST
Page 11 of 14


mixture)
SmIgM APC G20‐127 BD Biosciences 551062 B‐CLPD, BCP‐ALL 10
TCR PECy7 11F2 BD Biosciences EU: 655410 B‐CLPD, LST
3
US: 655434

Page 12 of 14


Panel 9. Composition of T‐CLPD and technical information on reagents

1 CD4 CD45 CD7 CD26 SmCD3 CD2 CD28 CD8
2 CD4 CD45 CD27 CD197 SmCD3 CD45RO CD45RA CD8
3 CD4 CD45 CD5 CD25 SmCD3 HLADR CyTcL1 CD8
4 CD4 CD45 CD57 CD30 SmCD3 CD11c CD8
5 CD4 CD45 CyPerforin CyGranzyme B SmCD3 CD16 CD94 CD8
6 CD4 CD45 CD279 SmCD3 CD8

Catalogue
number
CD2 PECy7 S5.2 BD Biosciences 335821 T‐CLPD 2
SmCD3 PerCPCy5.5 SK7 BD Biosciences 332771 T‐CLPD, NK‐CLPD 10
CD4 PacB RPA‐T4 BioLegend 300521 T‐CLPD, LST, B‐CLPD 0.5
CD5 FITC L17F12 BD Biosciences 345781 T‐CLPD 10
CD7 FITC 4H9 BD Biosciences 347483 T‐CLPD, NK‐CLPD 10
CD8 APCH7 SK1 BD Biosciences 641400 T‐CLPD 5
CD11c APC S‐HCL‐3 BD Biosciences 333144 T‐CLPD, NK‐CLPD 2
CD16 PECy7 3G8 BD Biosciences 557744 T‐CLPD 2
CD25 PE 2A3 BD Biosciences 341011 T‐CLPD, AML, NK‐CLPD 10
CD26 PE L272 BD Biosciences 340423 T‐CLPD, NK‐CLPD 10
CD27 FITC L128 BD Biosciences 340424 T‐CLPD 10
CD28 APC CD28.2 BD Biosciences 559770 T‐CLPD 10
CD30 PE BerH8 BD Biosciences 550041 T‐CLPD 10
CD45 PacO HI30 Invitrogen MHCD4530 T‐CLPD, ALOT, LST, SST, BCP‐ALL, T‐ 5
ALL, AML, NK‐CLPD
CD45RA APC HI100 BD Biosciences 550855 T‐CLPD 10
CD45RO PECy7 UCHL1 BD Biosciences 337168 T‐CLPD 2
CD57 FITC HNK‐1 BD Biosciences 333169 T‐CLPD, NK‐CLPD 10
CD94 APC HP‐3D9 BD Biosciences 559876 T‐CLPD, NK‐CLPD 5
CD197 PE 3D12 eBioscience 12‐1979 T‐CLPD 10
CD279 PE MIH4 BD Biosciences 557946 T‐CLPD 20
CyGranzyme B PE CLB‐GB11 Sanquin M2289 T‐CLPD, NK‐CLPD 15
HLADR PECy7 L243 BD Biosciences 335830 T‐CLPD 2.5
CyPerforin FITC G6 BD Biosciences 556577 T‐CLPD, NK‐CLPD 10
CyTCL1 APC eBio1‐21 eBioscience 17‐6699 T‐CLPD 2

Page 13 of 14


Panel 10. Composition of NK‐CLPD and technical information on reagents

1 CD2 CD45 CD7 CD26 SmCD3 CD56 CD5 CD19
2 CD16 CD45 CD57 CD25 SmCD3 CD56 CD11c CD19
3 HLADR CD45 CyPerforin CyGranzyme B SmCD3 CD56 CD94 CD19

Catalogue
number
CD2 PacB TS1/8 BioLegend 309216 NK‐CLPD 1
SmCD3 PerCPCy5.5 SK7 BD Biosciences 332771 NK‐CLPD, T‐CLPD 10
CD5 APC L17F12 BD Biosciences 345783 NK‐CLPD 2.5
CD7 FITC 4H9 BD Biosciences 347483 NK‐CLPD, T‐CLPD 10
CD11c APC S‐HCL‐3 BD Biosciences 333144 NK‐CLPD, T‐CLPD 2
CD16 PacB 3G8 BioLegend 302032 NK‐CLPD 5
CD19 APCH7 SJ25C1 BD Biosciences 641395 NK‐CLPD, AML 5
CD25 PE 2A3 BD Biosciences 341011 NK‐CLPD, AML, T‐CLPD 10
CD26 PE L272 BD Biosciences 340423 NK‐CLPD, T‐CLPD 10
CD45 PacO HI30 Invitrogen MHCD4530 NK‐CLPD, ALOT, LST, SST, BCP‐ 5
ALL, T‐ALL, AML, T‐CLPD
CD56 PECy7 N901 Beckman Coulter A21692 NK‐CLPD, T‐ALL 5
CD57 FITC HNK‐1 BD Biosciences 333169 NK‐CLPD, T‐CLPD 10
CD94 APC HP‐3D9 BD Biosciences 559876 NK‐CLPD, T‐CLPD 5
CyGranzyme B PE CLB‐GB11 Sanquin M2289 NK‐CLPD, T‐CLPD 15
HLADR PacB L243 BioLegend 307624 NK‐CLPD, AML 1 (1:5 dilution)
CyPerforin FITC G6 BD Biosciences 556577 NK‐CLPD, T‐CLPD 10

Page 14 of 14

Composition of EuroFlow Panels and Technical Information On Reagents Version 1.4

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Composition of EuroFlow Panels and Technical Information On Reagents Version 1.4

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PacB PacO FITC PE PerCPCy5.5 PECy7 APC APCH7

PacB PacO FITC PE PerCPCy5.5 PECy7 APC APCH7

CD20 PacB 2H7 BioLegend 302320 LST, SST, BCP‐ALL, B‐CLPD 1

PacB PacO FITC PE PerCPCy5.5 PECy7 APC APCH7

CD20 PacB 2H7 BioLegend 302320 SST, LST, BCP‐ALL, B‐CLPD 1

Tube PacB PacO FITC PE PerCPCy5.5 PECy7 APC APCC750

1 CD45 CD138 CD38 CD56 2micro CD19 CyIg CyIg

CD45 PacB T29/33 Dako PB986 PCD 5

Tube PacB PacO FITC PE PerCPCy5.5 PECy7 APC APCH7

Tube PacB PacO FITC PE PerCPCy5.5 PECy7 APC APCH7

1 CyCD3 CD45 NuTdT CD99 CD5 CD10 CD1a SmCD3

Tube PacB PacO FITC PE PerCPCy5.5 PECy7 APC APCH7

Tube PacB PacO FITC PE PerCPCy5.5 PECy7 APC APCH7

Tube PacB PacO FITC PE PerCPCy5.5 PECy7 APC APCH7

Tube PacB PacO FITC PE PerCPCy5.5 PECy7 APC APCH7

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