91421, 538 PM DETECTORS Nitrogen/Phophorus Detector - NPD Theory of Operation l capnut NPD heater —. block: HPO baad tt fats bo ionization area of the detector body) Electrical leads provide current to the NPD— bbead to maintain the hydrogen-air plasma Rrea where ionization takes place NPDIet Detector viewport JB. — colector electrode Positively charged Electrical lead toampliier Inside the NPD detector body, an electrically heated thermionic bead (NPD bead) is positioned between the jt orifice andthe collector electrode. The bead is coated with an alkali metal which promotes the ionization of compounds that contain nitrogen or phosphorus, Hydrogen and air lows ereate a hydrogen plasma around, thehot NPD bead. When molecules containing nitrogen or phosphorus enter the plasma from the column and jetori they undergo a catalytic surface chemistry reaction, producing thermionic electrons, Therresulting ions are attracted toa positively charged collector electrode, then amplified and output to the data system. The hydrogen to ar ratio is too lean to sustain aflame, therefore minimizing hydrocarbon ionization and contributing, tothe NPD detector’s selectivity. Detector viewport Hydrogen ‘Sample-laden carier gas flowing plasma, Positively charged collector from the column to the jet Stainless steo! jet electrode doubles as the ‘exhaust vent io Thermionic NPO bead N a Compressed airfoeds the nyerogen fame 4291421, 538 PM DETECTORS Nitrogen/Phophorus Detector - NPD Expected Performance ‘The following chromatograms are from an SRI GC equipped with NPD, DELCD and FID detectors Sincethe NPD isnot the only detector, helium carrier gas was used instead of nitrogen. The first chromatogram, showsa separation of 200ppm Organophosphorus pesticide standard, Mix 8270. The second chromatogram, shows both the NPD and DELCD responses to a mixture of 100ppm Mix 8270 and 100ppm Organochilorine pesticide standard, Mix 8081. Other than the sample and length of time, the analytical parameters were the same for both runs. The NPD has a much smaller response to the Organochlorine standard (Mix 8081). Since the DELCD is selective to chlorinated molecules, its response to Mix 8081 supplements the NPD response for better identification. and quantification. Sample: ipl 200ppm |OsUeiS \HLUL[v?r sss ealnnny COrganophophorus standard 7 rmx8270 zm Method: dirctinjection 0-37 minutes Column: 60-meter MXT-VOL Cari: helium at Om inin NPD gain: HIGH NPD temperature: 250°C NPD bead current: -370 Injector temperature: 150°C Temperature program: Initial Hold Ramp Final 1000 600 1500 2e0C 280°C 20.00 0.00 280°C Sample: 1yL_100ppm ‘Organophophorus pesticide standard mix 8270 + 100ppm Organochlorine pesticide standard mix 8081 Method: direct injection Injector temperature: 150°C ‘Column: 60-meterMXT-VOL, ‘Cari: helium at 1OmLfmin NPD gain: HIGH NPD temperature: 250°C NPO bead current:-370 DELCO gain: LOW DELCO reactor: 260 DELCO temp: 300°C a @ peLcp 22