Abx (P60)

Pentra 60
Technical Manual
P/n: RAA016B
ABX DIAGNOSTICS
B.P. 7290
Rue du caducée
Parc Euromédecine
34184 MONTPELLIER Cedex 04 - FRANCE
• REVISIONS
INDEX P/N REVISION REVISION SECTION DATE
A Creation all 19/07/99
B RAN062A Update all 25/05/04
• CONTENTS
1. SPECIFICATIONS
2. HYDRAULIC & PNEUMATIC PRINCIPLES
3. ELECTRIC & ELECTRONIC PRINCIPLES
4. ANALYSIS CYCLE TECHNOLOGY
5. SOFTWARE VERSION
6. OUTPUT FORMAT
7. ALARMS & ERROR LIST
8. MAINTENANCE PROCEDURES
9. TRAINING SLIDES
10. BLOWUPS VIEWS
11. SPARE PART LIST
Technical Manual RAA016 B
SPECIFICATIONS
1. Technical specifications ..................................................................................... 1-2

1.1. Parameters ......................................................................................................................... 1-2
1.1.1. CBC Mode ............................................................................................................ 1-2
1.1.2. CBC + 5DIFF Mode ............................................................................................... 1-3
1.1.3. Units ..................................................................................................................... 1-4
1.2. Throughput Analyses .......................................................................................................... 1-5
1.3. Tube Identification .............................................................................................................. 1-5
1.4. Reagents ............................................................................................................................ 1-5
1.5. Keyboard & Display ........................................................................................................... 1-5
1.6. Data processing ................................................................................................................. 1-5
1.7. Measurements and computation ........................................................................................ 1-5
2. Physical specifications ....................................................................................... 1-6

2.1. Power requirements ........................................................................................................... 1-6
2.2. Operating temperature and humidity .................................................................................. 1-6
2.3. Dimensions and Weight ...................................................................................................... 1-6
2.4. Minimum specimen volume ............................................................................................... 1-6
2.5. Dilution ratios ..................................................................................................................... 1-7
2.6. Hgb measurement .............................................................................................................. 1-7
2.7. Counting aperture diameters .............................................................................................. 1-7
2.8. Reagent consumption (ml) .................................................................................................. 1-8
3. Summary of performance data ........................................................................... 1-9

3.1. Precision (Reproducibility)* ................................................................................................ 1-9
3.2. Precision (Repeatability) ................................................................................................... 1-10
3.3. Linearity* .......................................................................................................................... 1-11
3.4. Carryover ......................................................................................................................... 1-11
3.5. Normal ranges* ................................................................................................................ 1-12
3.6. Accuracy* ........................................................................................................................ 1-13
3.7. Leukocyte differential count ............................................................................................. 1-13
3.8. Sample stability study ..................................................................................................... 1-13
3.9. Flags giving an analysis default ....................................................................................... 1-14
4. Reagents specifications ................................................................................... 1-15

4.1. Reagent specifications ..................................................................................................... 1-15
4.2. Waste handling precautions ............................................................................................. 1-15
5. Limitations ........................................................................................................ 1-16

5.1. Maintenance .................................................................................................................... 1-16
5.2. Blood specimens ............................................................................................................. 1-16
5.3. Known interfering substances .......................................................................................... 1-17
page 3/8
2-HYDRAULIC & PNEUMATIC PRINCIPLES

1. Pentra 60 Hydraulic ............................................................................................ 2
1.1. Transmission tube list ........................................................................................................ 2
1.2. Hydropneumatic connections ............................................................................................ 4
1.3. Functions of valves ............................................................................................................ 7
2. Pneumatic diagrams .......................................................................................... 8

2.1. Pentra 60 general pneumatic diagram ................................................................................ 8
2.2. Lyse circuit ........................................................................................................................ 9
2.3. Eosinofix circuit ............................................................................................................... 10
2.4. Basolyse II circuit ............................................................................................................ 11
2.5. Cleaner circuit .................................................................................................................. 12
2.6. Diluent circuit ................................................................................................................... 13
2.7. Waste circuit .................................................................................................................... 14
3-ELECTRIC & ELECTRONIC PRINCIPLES

1. Pentra 60 Main Board ............................................................................................. 2
1.1. Main board general view ....................................................................................................... 2
1.2. Main board Test Points ......................................................................................................... 3
1.3. Main board Potentiometers ................................................................................................... 5
1.4. Main Board configuration ...................................................................................................... 6
2. Pentra 60 Boards ..................................................................................................... 7

2.1. Optical preampli board XAA423B .......................................................................................... 7
2.2. LCD & Keyboard board XAA426B ......................................................................................... 8
2.3. Motor relay board XAA427B .................................................................................................. 9
2.4. Carriage board XAA428B ..................................................................................................... 10
2.5. LED board XAA429B ........................................................................................................... 11
3. Connections .......................................................................................................... 12
3.1. Chamber Heating Connector XBA386A ................................................................................ 12
3.2. Reagent Heating Coil Connector XBA387A .......................................................................... 13
3.3. Horizontal Carriage Motor XBA391A ..................................................................................... 14
3.4. Upper Fan XBA393A ........................................................................................................... 15
3.5. IR sensor XBA394AS ........................................................................................................... 16
3.6. IR sensor XBA395AS ........................................................................................................... 17
3.7. IR sensor XBA396AS ........................................................................................................... 18
3.8. IR sensor XBA397AS ........................................................................................................... 19
3.9. RBC / WBC coaxe XBA398A ............................................................................................... 20
3.10. LMNE flowcell coaxe XEA663A (XBA399B+XBA490A) ....................................................... 21
3.11. Barcode reader cable XBA402AS ...................................................................................... 22
3.12. Diluent level sensor XDA605AS ......................................................................................... 23
4. Pentra 60 Synoptic ................................................................................................ 24
page 4/8
4-ANALYSIS CYCLE TECHNOLOGY

1. Analysis cycle description .................................................................................. 2
2. Measuring principles .......................................................................................... 4

2.1. Multi Distribution Sampling System (MDSS) ....................................................................... 4
2.2. RBC / PLT detection principles .......................................................................................... 5
2.3. Hgb measurement ............................................................................................................. 6
2.4. Hct measurement .............................................................................................................. 6
2.5. RDW Calculation ................................................................................................................ 6
2.6. MCV, MCH, MCHC Calculation .......................................................................................... 7
2.7. MPV Measurement ............................................................................................................ 7
2.8. Thrombocrit calculation ...................................................................................................... 7
2.9. PDW calculation ................................................................................................................ 8
2.10. WBC count and differentiation ........................................................................................... 8
2.11. Dilutions summary ........................................................................................................... 12
5-SOFTWARE VERSIONS
1. Software Overview: User Menu Overview .......................................................... 2
2. Software Overview: Technician Menu Overview ................................................ 3
3. Software Release ............................................................................................... 4
6-OUTPUT FORMAT
page 5/8
7-ALARMS & ERROR LIST
1. Printer ................................................................................................................. 2
2. Transmission ...................................................................................................... 2
3. Calibration .......................................................................................................... 2
4. Technical Operations .......................................................................................... 3
5. Reagents ............................................................................................................ 3
6. Temperature ........................................................................................................ 3
8-MAINTENANCE PROCEDURES
1. Maintenance ...................................................................................................... 2
1.1. Introduction ........................................................................................................................ 2
1.2. Daily customer maintenance .............................................................................................. 2
1.3. Weekly customer maintenance .......................................................................................... 2
1.4. Maintenance table ............................................................................................................. 3
2. Maintenance kits ................................................................................................ 4

2.1. 6 month Maintenance kit .................................................................................................... 4
2.2. Yearly Maintenance kit ....................................................................................................... 4
2.3. Pistons Maintenance kit ..................................................................................................... 5
2.4. Fitting Kit ........................................................................................................................... 5
2.5. Screws Kit ......................................................................................................................... 6
3. Procedures ......................................................................................................... 8
3.1. Procedure chart tables ....................................................................................................... 9
3.2. Required tools and products ........................................................................................... 10
page 6/8
9-TRAINING SLIDES
10-BLOWUPS VIEWS
1. Blowups views .................................................................................................... 1

1.1. 11 Valves assy .................................................................................................................. 1
1.2. 5 Valves assy (12-16) ......................................................................................................... 2
1.3. 2 Valves assy .................................................................................................................... 3
1.4. 7 Valves assy .................................................................................................................... 4
1.5. 5 Valves assy (27-31) ......................................................................................................... 5
1.6. Optical bench syringe assy ............................................................................................... 6
1.7. Reagents syringe assy ...................................................................................................... 7
1.8. Sampling syringe assy ....................................................................................................... 8
1.9. Syringe motorization carriage assy ..................................................................................... 9
1.10. Syringe motor assy .......................................................................................................... 10
1.11. Syringe motor body assy ................................................................................................. 11
1.12. Sampling motor assy ....................................................................................................... 12
1.13. Optical syringe motor assy .............................................................................................. 13
1.14. Reagents motor assy ....................................................................................................... 14
1.15. Sampling syringe assy ..................................................................................................... 15
1.16. Vacuum syringe piston assy ............................................................................................ 16
1.17. Vacuum syringe assy ....................................................................................................... 17
1.18. Short vacuum syringe assy .............................................................................................. 18
1.19. Long vacuum syringe ....................................................................................................... 19
1.20. Long vacuum syringe assy .............................................................................................. 20
1.21. Short vacuum syringe assy .............................................................................................. 21
1.22. Needle carriage motor assy ............................................................................................. 22
1.23. Horizontal sampling carriage notched belt ....................................................................... 23
1.24. Notched belt .................................................................................................................... 24
1.25. Needle carriage assy ....................................................................................................... 25
1.26. Sampling horizontal carriage ............................................................................................ 26
1.27. Sampling horizontal carriage assy .................................................................................... 27
1.28. Sampling carriage assy ................................................................................................... 28
1.29. Carriage vertical belt ........................................................................................................ 29
1.30. Carriage rinsing block assy .............................................................................................. 30
1.31. Home carriage cell ........................................................................................................... 31
1.32. Needle assy .................................................................................................................... 32
1.33. Carriage motor assy ......................................................................................................... 33
1.34. Sampling needle assy ..................................................................................................... 34
1.35. Reagents heating coils assy ............................................................................................ 35
1.36. Thermostated room assy ................................................................................................. 36
1.37. Thermostated room plate assy ........................................................................................ 37
1.38. Thermostated room fan assy ........................................................................................... 38
1.39. Frame assy ...................................................................................................................... 39
1.40. Diluent chamber assy ...................................................................................................... 40
1.41. Diluent chamber .............................................................................................................. 41
1.42. RETIC flowcell assy ......................................................................................................... 42
1.43. Optical bench assy ......................................................................................................... 43
1.44. Optical bench lamp ......................................................................................................... 44
1.45. Preamplifier support ........................................................................................................ 45
page 7/8
11-SPARE PART LIST
1. Spare part list ...................................................................................................... 1
page 8/8
SPECIFICATIONS
1. TECHNICAL SPECIFICATIONS ................................................. 1-2

1.1. Parameters ....................................................................... 1-2
1.1.1. CBC Mode ................................................................ 1-2
1.1.2. CBC + 5DIFF Mode ................................................... 1-3
1.1.3. Units .............................................................................. 1-4
1.2. Throughput Analyses ....................................................... 1-5
1.3. Tube Identification ............................................................ 1-5
1.4. Reagents ......................................................................... 1-5
1.5. Measurements and computation ..................................... 1-5
2. PHYSICAL SPECIFICATIONS ................................................... 1-6
2.1. Power requirements ........................................................ 1-6
2.2. Operating temperature and humidity .............................. 1-6
2.3. Dimensions and Weight .................................................. 1-6
2.4. Minimum specimen volume ............................................ 1-6
2.5. Dilution ratios .................................................................. 1-7
2.6. Hgb measurement .......................................................... 1-7
2.7. Counting aperture diameters ........................................... 1-7
2.8. Reagent consumption (ml) .............................................. 1-8
3. SUMMARY OF PERFORMANCE DATA .................................... 1-9
3.1. Precision (Reproducibility)* .............................................. 1-9
3.2. Precision (Repeatability) ................................................. 1-10
Precision claims* .............................................................. 1-10
3.2. Linearity* ........................................................................ 1-11
3.3. Carryover* ...................................................................... 1-11
3.4. Normal ranges* .............................................................. 1-12
3.5. Accuracy* ...................................................................... 1-13
3.6. Leukocyte differential count ........................................... 1-13
3.7. Sample stability study ..................................................... 1-13
3.8. Flags giving an analysis default ...................................... 1-14
4. REAGENTS SPECIFICATIONS ................................................ 1-15
4.1. Reagent specifications .................................................... 1-15
4.2. Waste handling precautions ........................................... 1-15
5. LIMITATIONS .......................................................................... 1-16
5.1. Maintenance ................................................................... 1-16
5.2. Blood specimens ........................................................... 1-16
5.3. Known interfering substances ........................................ 1-17
1. Specifications RAA016B
1. TECHNICAL SPECIFICATIONS
• The ABX PENTRA 60 is a fully automated hematology analyzer used for in vitro diagnostic
testing of whole blood specimens.
• The ABX PENTRA 60 is able to operate either in CBC mode (Cell Blood Count:12 parameters)
or in CBC + 5DIFF mode (5 population Differential count: 26 parameters).
1.1. Parameters
1.1.1. CBC Mode
WBC White Blood Cell
RBC Red Blood Cell

Hgb Hemoglobin concentration
Hct Hematocrit
MCV Mean Corpuscular Volume
MCH Mean Corpuscular Hemoglobin
MCHC Mean Corpuscular Hemoglobin Concentration
RDW Red Distribution Width
Plt Platelets
PDW * Platelet Distribution Width
MPV Mean Platelet Volume
Pct * Plateletcrit
* PDW and PCT have not been established as indications for this product,in the Uni-
ted States. The use of PCT and PDW should be restricted to research and
Investigational measurements only.
page 2/20
1.1.2. CBC + 5DIFF Mode
WBC White Blood Cell

LYM Lymphocytes % and #
MON Monocytes % and #
NEU Neutrophils % and #
EOS Eosinophils % and #
BAS Basophils % and #
LIC * Large Immature Cell % and #
ALY * Atypical Lymphocyte % and #
RBC Red Blood Cell

Hgb Hemoglobin concentration
Hct Hematocrit
MCV Mean Corpuscular Volume
MCH Mean Corpuscular Hemoglobin
MCHC Mean Corpuscular Hemoglobin Concentration
RDW Red Distribution Width
Plt Platelets
PDW * Platelet Distribution Width
MPV Mean Platelet Volume
Pct * Plateletcrit
* PCT, PDW, ALY and LIC have not been established as indications for this product,in
the United States. The use of PCT, PDW, ALY and LIC should be restricted to research
and Investigational measurements only.
page 3/20
1.1.3. Units
UNITS
WBC RBC HGB HCT PLT MCV
STD 103/mm3 106/mm3 g/dl % 103/mm3 µm3
SI 109/l 1012/l g/l l/l 109/l fl
mmol/l 109/l 1012/l mmol/l l/l 109/l fl
MCH MCHC RDW MPV PCT PDW

3
STD pg g/dl % µm % %
SI pg g/l % fl 10-2/l %
mmol/l fmol mmol/l % fl 10-2/l %
LYC LYC MON MON NEU NEU EOS

STD % # % # % # %
SI % # % # % # %
mmol/l % # % # % # %
EOS BAS BAS ALY ALY LIC LIC

STD # % # % # % #
SI # % # % # % #
mmol/l # % # % # % #
page 4/20
1.2. Throughput Analyses

• CBC Mode (CBC): .............. 60/h
• CBC + 5DIFF Mode (DIF): .. 60/h
1.3. Tube Identification

• By means of the worklist or identification file.
• By means of the Barcode labels.
1.4. Reagents
• ABX DILUENT (20 litres),
• ABX CLEANER (1 litre, Integrated),
• ABX EOSINOFIX (1litre, Integrated),
• ABX BASOLYSE II (1 litre, Integrated),
• ABX ALPHALYSE or LYSEBIO (0.4 litre, Integrated).
1.5. Keyboard & Display

• ABX specific keyboard, silicone made.
• LCD, 128 x 240 pixels, LED Backlighted.
1.6. Data processing

• 68331 type microprocessor.
• RS232C output.
1.7. Measurements and computation

• Impedance for WBC, Plt, RBC, BASO.
• Photometry for Hgb.
• Impedance and light scattering for LYM, MON, NEU, EOS, ALY and LIC.
• Computation from stored data that was directly measured for Hct, MCV, MCH, MCHC,
RDW, MPV, Pct, PDW.
page 5/20
2. PHYSICAL SPECIFICATIONS
2.1. Power requirements

• Power supplies: ................. * from 100Vac to 240Vac (+10%)
.................................. * 50 Hz to 60 Hz
• Power Consumption: ......... * Analyzer: 200 VA

.................................. * Printer (Depends on the printer, see Printer’s manual)
2.2. Operating temperature and humidity

• 16 - 34°C (61 - 93°F) room temperature.
• Maximum relative humidity 80% for temperatures up to 31°C (88°F) decreasing linearly to
50% relative humidity at 40°C (104°F).
2.3. Dimensions and Weight

• Analyzer dimensions: ......... * Height: approximately 516 mm (20.3 in.)
.................................. * Width: approximately 444 mm (17.5 in.)
.................................. * Depth: approximately 481 mm (19 in.)
• Analyzer weight: ................. * approximately 35Kgs (77lbs)
2.4. Minimum specimen volume

• CBC Mode (CBC): .............. * 30µl
• CBC + 5DIFF Mode (DIF): .. * 53µl
page 6/20
2.5. Dilution ratios

• WBC/BASO ........................ * 1/200
• LMNE ................................. * 1/80
• RBC/Plt .............................. * 1/10 000
• Hgb .................................. * 1/250
2.6. Hgb measurement

• Hgb chamber, LED 555 nm.
• Modified Drabkin method (cyanmethemoglobin)
• Light source ....................... * Electroluminescent diode

• Wavelength ........................ * 550nm + 10nm
2.7. Counting aperture diameters

• WBC/BASO ........................ * 80 µm
• LMNE ................................. * 60 µm
• RBC/Plt .............................. * 50 µm
page 7/20
2.8. Reagent consumption (ml)

CYCLE REAGENT APPROXIMATE
DILUENT BASOLYSE II CLEANER EOSINOFIX ALPHALYSE DURATION
CBC Cycle 20.4 2.1 0.9 x 0.4 60’’
Diff Cycle 25.6 2.1 0.9 1 0.4 60’’
Startup* 60.8 2.1 3.7 1 1.4 3’53’’
Shutdown 20.5 x 14 x 1 2’48’’
Prime Diluent 42.9 x x x x 3’03’’
Prime Cleaner 1.1 x 24.8 x x 1’22’’
Prime Eosinofix 1.6 x x 23.6 x 1’12’’
Prime Basolyse II 1.1 23.6 1.1 x x 1’20’’
Prime alphalyse 2.1 x x x 8.4 1’27’’
Prime all reagents 47 24 25.1 24 8.2 6’
Autoclean cycle 14.2 1 1 1 0.3 1’38’’
Autocontrol cycle 23.4 x 1.4 x 1 1’4’’
RBC chamber cleaning 2.5 x x x x 7’’
Unprime all x x x x x 6’25’’
Chambers rinsing 12.6 1 1 1 0.3 1’17’’
Cytometer rinsing 4.9 x x x x 1’11’’
Concentrated cleaning 25 x 1.4 x 0.9 4’10’’
Cleaning 12.6 1 1 1 0.3 1’19’’
* for one background count only (maxi = 3)
page 8/20
3. SUMMARY OF PERFORMANCE DATA

3.1. Precision (Reproducibility)*
• The instrument was initially calibrated with Minocal Calibrator (Lot No. CX322, Expiry
Date: 05-Aug-2002).
• Three levels of ABX MINOTROL material (Lot No: JX108) were run in duplicate once daily
for a prolonged period (26th July 2002 to 30 August 2002) on all parameters.
The results were used to quantify within run precision, and Total Precision in accordance
with the NCCLS EP 5-A Guidelines.
PARAMETER ABX MINOTROL WITHIN RUN SD OF RUN SD OF DAILY TOTAL

CONTROL SD MEANS MEANS PRECISION (SD)
JX108 Low 0.001 N/A N/A 0.001
WBC JX108 Normal 0.008 N/A N/A 0.013
JX108 High 0.031 N/A N/A 0.045
JX108 Low 0.000 N/A N/A 0.000
RBC JX108 Normal 0.001 N/A N/A 0.001
JX108 High 0.002 N/A N/A 0.003
JX108 Low 0.001 N/A N/A 0.001
HGB JX108 Normal 0.002 N/A N/A 0.007
JX108 High 0.005 N/A N/A 0.012
JX108 Low 0.021 N/A N/A 0.025
HCT JX108 Normal 0.064 N/A N/A 0.122
JX108 High 0.104 N/A N/A 0.181
JX108 Low 6.271 N/A N/A 20.646
PLT JX108 Normal 40.229 N/A N/A 72.103
JX108 High 154.146 N/A N/A 381.388
PARAMETER ABX MINOTROL WITHIN RUN CV% OF RUN CV% OF DAILY TOTAL
CONTROL CV% MEANS MEANS PRECISION (CV%)
JX108 Low 1.8 N/A N/A 1.93
WBC JX108 Normal 0.9 N/A N/A 1.12
JX108 High 0.9 N/A N/A 1.05
JX108 Low 0.8 N/A N/A 0.86
RBC JX108 Normal 0.6 N/A N/A 0.79
JX108 High 0.7 N/A N/A 0.88
JX108 Low 0.4 N/A N/A 0.57
HGB JX108 Normal 0.3 N/A N/A 0.59
JX108 High 0.4 N/A N/A 0.60
JX108 Low 0.9 N/A N/A 0.99
HCT JX108 Normal 0.7 N/A N/A 0.97
JX108 High 0.7 N/A N/A 0.89
JX108 Low 3.1 N/A N/A 5.69
PLT JX108 Normal 2.6 N/A N/A 3.46
JX108 High 2.5 N/A N/A 4.01
*Source: 510K submission K030144
page 9/20
Precision claims*
PARAMETER % CV NOMINAL VALUES

WBC < 2.0% 10.0 x 103/mL
RBC < 2.0% 4.67 x 106/mL
HGB < 1.0% 13.6 g/dl
HCT < 2.0% 36.0 %
PLT < 5.0% 243 x 103/mL
3.2. Precision (Repeatability)

• Based on 10 consecutive samples of the same fresh whole blood sample without alarm:
PARAMETERS %CV TEST LEVEL
WBC <2% at 10x103/µL

RBC <2% at 5x106/µL
Plt <5% at 300x103/µL
Hgb <1% at 15 g/dL
Hct <2% at 45%
page 10/20
3.3. Linearity*
• Linearity range: The Manufacturer’s tested linearity zone of the instrument using linearity
kits and/or human blood.
• Linearity limits: Maximum and minimum values within instrument returns no dilution alarm.
• Visible range: Range values given by the instrument. These values (above linearity limits)
are given as an indication. They are given associated with a «D» flag. This Visible range is
outside Manufacturer’s range.
• Linearity kits
Linearity was tested using available «Low Range» and «Full Range» Linearity Test kits. The
Test kits were analyzed and data was computed according to the Manufacturer’s instruc-
tions.
• Human Blood
Linearity was also obtained on human blood, using a minimum of 5 dilution points. The
re-sults of this study are as followed:
PARAMETER LINEARITY LINEARITY VISIBLE DIFFERENCE

RANGE LIMITS RANGE
WBC (103/µL) 0.40 – 130.80 0 – 120 120 – 150 ±0.3 ±7%

RBC (106/µL) 0.23 – 9.76 0 – 8.00 8.00 – 18.00 ±0.07 ±2%
HGB (g/dL) 0.00 – 31.06 0 – 24 24 – 30 ±0.3 ±2%
HCT (%) 1.80 – 88.90 0 – 67 67 – 80 ±2.0 ±3%
PLT (103/µL) 3.30 – 2007 0 – 1900 1900 – 2800 ±10 ±10%
PLT On platelet concentrate** 7.00 – 2895 0 – 2800 2800 – 3200 ±10 ±10%
(103/µL)

**The instrument must have been configured by an ABX DIAGNOSTICS approved technician.
3.4. Carryover
• The ABX PENTRA 60 carry-over effects were evaluated by assaying a sample with high cell
concentrations three consecutive times (i1-3), followed immediately by testing a diluted sample
consecutively 3 times (j1-3).
(j1-j3)
Carryover = X 100
(i3-j3)
Carry-over % is then:
WBC RBC HGB PLT

Mean low levels 1.06 1.58 5.28 31.33
Mean high levels 58.81 6.37 22.03 1106.67
Carry over % -0.260 0.000 -0.179 -0.186
This is the method as described in Guidelines for the Evaluation of blood cell analyzers
including those used for differential leukocyte and reticulocyte counting and cell marker
applications. ISLH, 14 January, 1994.
page 11/20
• Carry-over Conclusion:
Results provided are extremely satisfactory. In order to provide for eventual possibilities
within the laboratory environment the following claims shall be made :
WBC RBC HGB PLT

Claims < 2.0% < 2.0% < 2.0% < 2.0%
3.5. Normal ranges*

PARAMETERS MALE FEMALE
WBC (103/µL) 4 - 10 4 - 10
6
RBC (10 /µL) 4.50 - 6.50 3.80 - 5.80
HGB (g/dL) 13.0 - 17.0 11.5 - 16.0
HCT (%) 40.0 - 54.0 37.0 - 47.0
MCV (µm3) 80 - 100 80 - 100
MCH (pg) 27.0 - 32.0 27.0 - 32.0
MCHC (g/dL) 32.0 - 36.0 32.0 - 36.0
RDW (%) 11.0 - 16.0 11.0 - 16.0
PLT (103/µL) 150 - 500 150 - 500
MPV (µm3) 6 - 11 6 - 11
PCT (%) 0.15 - 0.50 0.15 - 0.50
PDW (%) 11 - 18 11 - 18
NEU (%) 50 - 80 50 - 80
LYM (%) 25 - 50 25 - 50
MON (%) 2 - 10 2 - 10
EOS (%) 0-5 0-5
BAS (%) 0-2 0-2
IMPORTANT
Expected values will vary with sample population and/or geographical
location. It is highly recommended that each Laboratory establish its
own Normal ranges based upon the local population!
*Bibliography:
AIDE MEMOIRE D’HEMATOLOGIE
Prof : C.SULTAN / M. GOUAULT- HELMANN / M. IMBERT
Service Central d’Hématologie de l’Hopital Henri Mondor
Faculté de médecine de Créteil (Paris XII)
page 12/20
3.6. Accuracy*
• The data shows good correlation between results achieved on the ABX PENTRA 60 versus
the reference system, which can be resumed as follows:
PARAMETER R²(COMPARISON OF MEANS) ACCURACY CLAIMS

WBC 0.997 >0.95
PLT 0.998 >0.95
RBC N/A N/A
HGB N/A N/A
HCT N/A N/A
LYM N/A N/A
NEU N/A N/A
MON N/A N/A
EOS N/A N/A
BAS N/A N/A
3.7. Leukocyte differential count

• Not available at the time of publication.
3.8. Sample stability study

• Not available at the time of publication.
page 13/20
3.9. Flags giving an analysis default
Results displayed
PARAMETER LINEARITY VISIBLE >VISIBLE

LIMITS RANGE RANGE
WBC(x103/mm3) «result» «result»+ «D» DIL

RBC(x106 /mm3) «result» «result»+ «D» DIL
PLT(x103/mm3) «result» «result»+ «D» DIL
HGB(g/dL) «result» «result»+ «D» DIL
HCT(%) «result» «result»+ «D» DIL
Results transmitted and printed out
PARAMETER LINEARITY VISIBLE >VISIBLE

LIMITS RANGE RANGE
WBC(x103/mm3) «result» «result»+«D» --.-- + D

RBC(x106 /mm3) «result» «result»+«D» --.-- + D
PLT(x103/mm3) «result» «result»+«D» --.-- + D
HGB(g/dL) »result» «result»+ «D» --.-- + D
HCT(%) «result» «result»+ «D» --.-- + D
NOTE
Use the instrument diluent to dilute the sample if a ---- D flag occurs
on WBC or Hct.
page 14/20
4. REAGENTS SPECIFICATIONS
4.1. Reagent specifications

• In order for the instrument to operate correctly, high-quality reagents must be used. ABX
DIAGNOSTICS provides all the necessary reagents.
NOTE
The ABX Diagnostics reagents specified for this instrument may have
followed one or both of the approval methods below:
1 - have been registered by the A.F.S.S.A.P.S. «Agence Française de
Sécurité Sanitaire des Produits de Santé» according to the procedure
relative to laboratory reagents used for biological analyses.
2 - or approved in accordance with the European Directive 98/79/CE
(Annex III) for in-vitro medical devices.
Please refer to the packaging of each reagent concerned to establish
the approval method.
• Refer to Chapter Annex of this manual for all reagent speifications.
4.2. Waste handling precautions
WARNING
When disposing of waste, protective clothing must be worn (lab coat,
gloves, eye protection, etc…). Follow your local and /or national
guidelines for biohazard waste disposal.
• If required, waste can be neutralized before being discarded. Follow your laboratory’s
protocol when neutralizing and disposing of waste.
• Dispose of the waste container according to the local or national regulatory requirements.
page 15/20
5. LIMITATIONS
WARNING
Whilst every effort is taken by ABX Diagnostics to investigate and
indicate all known interference’s, it is by no means possible to guarantee
that all interference’s have been identified. At all times, results should
be validated and communicated only once all information relating to
the patient has been assessed and taken into account.
5.1. Maintenance
• In Chapter 5. Maintenance & Troubleshooting, specific maintenance procedures are listed.
The maintenance procedures identified are mandatory for proper use and operation of the
ABX PENTRA 60. Failure to execute any of these recommended procedures may result in poor
reliability of the system.
IMPORTANT
Failure to execute any of these recommended procedures may result
in poor reliability of the system.
5.2. Blood specimens

• Verification of any abnormal test result (including flagged results or results outside of the
normal range) should be performed using reference methods or other standard laboratory
procedures for conclusive verification of the results. The sections below list known limita-
tions of automated blood cell counters which use the principles of impedance and light
absorbance as principles of measurement.
page 16/20
5.3. Known interfering substances
White Blood Cells (Leukocytes):

WBC results that exceed the linearity limits of the system will require dilution of the blood
sample (Leukemia sample followed by a leukopenia).
Re-assaying the diluted sample will help to obtain the correct assay value.
Unlysed Red Cells - In some rare instances, the erythrocytes in the blood sample may not
be completely lysed. These non-lysed red blood cells may be detected on the WBC histogram
with an L1 alarm or as an elevated baseline on the side (leading edge) of the lymphocytes
population. Non-lysed erythrocytes will cause a falsely elevated WBC count.
Multiple myeloma - The precipitation of proteins in multiple myeloma patients may give
high WBC counts.
Leukemia - A very low WBC count may result from this disease because of possible increased
fragility of the leukocytes leading to destruction of some of these cells during counting.
These white cell fragments will also interfere with the white cell differential parameters.
These white cell fragments will also interfere with the white cell partial differential parameters:
LYM% + #, MON% + #, GRAN% + #. A suspiciously low WBC count may also be seen in
patients with lymphocytic leukemias due to the presence of abnormally small lymphocytes
which may not be counted by the instrument.
Chemotherapy - Cytotoxic and immunosuppressive drugs may increase the fragility of the
leukocytes which may cause low WBC counts.
Cryoglobulins - Increased levels of cryoglobulin that may be associated with myeloma,

carcinoma, leukemia, macroglobulinemia, lymphoproliferative disorders, metastic tumors,
autoimmune disorders, infections, aneurism, pregnancy, thromboembolic phenomena,
diabetes, etc, which can increase the WBC, RBC or PLT counts and the HGB concentration.
The specimen must be warmed up to 37°C (99°F) in a bain marie for 30 minutes and analyzed
again immediately after (analyzer or manual method).
Macrothrombocytes - in excessive numbers may affect and increase Leukocyte numeration.
RBC: Red Blood Cells (Erythrocytes)

The red blood cell dilution contains all the formed elements in the blood: erythrocytes,
leukocytes and platelets. During erythrocytes counting (red blood cells), platelets are not
counted as their size falls below the minimum threshold.
Agglutinated erythrocytes - May cause a low incorrect RBC count. Blood samples
containing the agglutinated red blood cells may be suspected by elevated MCH and MCHC
values and shown by examination of the stained blood film.
Cold agglutinins - IgM immunoglobulins which are high in cold agglutinin disease may
cause lower RBC and PLT counts and increase MCV.
page 17/20
Hgb (Hemoglobin):
Turbidity of the blood sample - Any number of physiological and/or therapeutic factors
may produce high incorrect HGB results. To obtain accurate hemoglobin results when
increased turbidity of the blood sample occurs, determine the cause of the turbidity and
follow the appropriate method below:
High WBC: An extremely high WBC will cause excessive light scatter. In these cases use
reference (manual) methods.The diluted sample should be centrifuged, and the supernatant
fluid measured with a spectrophotometer.
High lipid concentration: A high concentration of lipids in the blood sample will give the
plasma a “milky” appearance. This condition can occur with hyperlipidemia, hyperproteinemia
(as in gammapathies) and hyperbilirubinemia. Accurate hemoglobin determinations can be
achieved by using reference (manual) methods and a plasma blank.
Increased turbidity may also be seen in cases where the red blood cells are resistant to
lysing. This condition will cause an incorrect high HGB result, but may be detected by
observing the abnormal MCH, MCHC values, and the increased baseline on the leading
edge of the WBC histogram. Erroneous hemoglobin results will cause the results of the
MCH and MCHC to be incorrect as well.
Fetal bloods - The mixing of fetal and maternal bloods may produce a high inaccurate HGB
value.
Hct (Hematocrit)
Red blood cells agglutination - May produce an inaccurate HCT and MCV values. Red
blood cell agglutination may be detected by observing abnormal MCH and MCHC values,
as well as by examination of the stained blood film In such cases, manual methods may be
required to obtain an accurate HCT value.
MCV (Mean Corpuscular Volume)

Red blood cell agglutination - May produce an inaccurate MCV value. Red blood cell
agglutination may be detected by observing abnormal MCH and MCHC values, as well as
by examination of the stained blood film. In such cases, manual methods may be required to
obtain an accurate MCV value.
Excessive numbers of large platelets and/or the presence of an excessively high WBC
count may interfere with the accurate determination of the MCV value. In such cases, careful
examination of the stained blood film may reveal the error.
MCH (Mean Corpuscular Hemoglobin)

The MCH is determined according to HGB value and the RBC count. The limitations listed
for the HGB and RBC will have an effect on the MCH and may cause inaccurate values.
MCHC (Mean Corpuscular Hemoglobin Concentration)

The MCHC is determined according to the HGB and HCT values. The limitations listed for
the HGB and HCT will have an effect on the MCHC and may cause inaccurate values.
page 18/20
RDW (Red blood cell Distribution Width)

The red blood cell distribution width is determined according to the RBC count.
Nutritional deficiency or blood transfusion - May cause high RDW results due to iron
and/or cobalamin and /or folate deficiency.
Plt (Platelets)
Very small erythrocytes (microcytes), erythrocyte fragments (schizocytes) and WBC frag-
ments may interfere with the proper counting of platelets and cause elevated PLT counts.
Agglutinated erythrocytes - May trap platelets, causing an erroneously low platelet count.
The presence of agglutinated erythrocytes may be detected by observation of abnormal
MCH and MCHC values and by careful examination of the stained blood film.
Giant platelets in excessive numbers - may cause a low inaccurate platelet count as
these large platelets may exceed the upper threshold for the platelet parameter and are not
counted.
Chemotherapy - Cytotoxic and immunosuppressive drugs may increase the fragility of

these cells which may cause low PLT counts. Reference (manual) methods may be necessary
to obtain an accurate platelet count.
Hemolysis - Hemolysed specimens contain red cell stroma which may increase platelet
counts.
A.C.D. blood - Blood anticoagulated with acid-citrate-dextrose may contain clumped platelet
which could decrease the platelet count.
Elevated triglycerides and/or cholesterol: may interfere with correct platelet counting.
Platelet agglutination - Clumped platelets may cause a decreased platelet count and/or a
high WBC count. The specimen should be recollected in sodium citrate anticoagulant to
ensure the anticoagulated character depending on agglutination and reanalyzed only for the
platelet count. The final PLT result must be corrected for the sodium citrate dilution effect.
However, these platelet clumps do trigger flags L1, LL and LL1.
MPV (Mean Platelet Volume)

Giant platelets that exceed the upper threshold of the Platelet parameter may not be counted
as platelets. Consequently, these larger platelets will not be included in the instrument’s
calculation of Mean Platelet Volume.
Very small erythrocytes (microcytes), erythrocytic fragments (Schizocytes) and white blood
cell fragments may interfere with the proper counting and sizing of Platelets.
Agglutinated erythrocytes - May trap Platelets, causing an incorrect MPV result. The
presence of agglutinated erythrocytes may be detected by observation of abnormal MCH
and MCHC values and by careful examination of the stained blood film.
Chemotherapy - May also affect the sizing of PLTs.
IMPORTANT
Blood samples collected in EDTA will not maintain a stable Mean
Platelet Volume. Platelets collected in EDTA swell depending on the
time post-collection and storage temperature.
page 19/20
LYM# (Lymphocyte count absolute value), LYM% (Lymphocyte percentage)

The Lymphocyte count is derived from the WBC count. The presence of erythroblasts, cer-
tain parasites and erythrocytes that are resistant to lysis may interfere with an accurate LYM
count. Limitations listed for the WBC count pertain to the LYM # and % counts as well.
MON# (mononuclear cell count absolute), MON% (Mononuclear percentage)

The mononuclear cell count absolute is derived from the WBC count. The presence of large
lymphocytes, atypical lymphocytes, blasts and an excessive number of basophils may
interfere with an accurate monocyte count. Limitations listed for the WBC count pertain to
the MON # and % counts as well.
NEU# (neutrophil count absolute), NEU% (Neutrophil percentage)

The neutrophils cell count is derived from the WBC cell count. The excessive presence of
eosinophils, metamyelocytes, myelocytes, promyelocytes, blasts and plasma cells may
interfere with an accurate neutrophils count.
EOS# (Eosinophil cell count absolute), EOS% (Eosinophil percentage)

The eosinophil cell count is derived from the WBC cell count. The presence of abnormal
granules (degranulated areas, toxic granules...) may interfere with the eosinophil counting.
BAS# (Basophil cell count absolute), BAS% (Basophil percentage)

The Basophil cell count is derived from the WBC cell count.
page 20/20
HYDRAULIC&PNEUMATIC
PRINCIPLES
1. Pentra 60 Hydraulic ............................................................ 2

1.1. Transmission tube list .................................................................. 2
1.2. Hydropneumatic connections ...................................................... 4
1.3. Functions of valves ...................................................................... 7
2. Pneumatic diagrams .......................................................... 8

2. Hydraulic & Pneumatic principles RAA016B
1. PENTRA 60 HYDRAULIC
Liquid circuits, hydropneumatic connections, as well as the transmission tubes
used, are described in the following chart tables.
1.1. Transmission tube list
PART
DESIGNATION DIAMETER LENGTH QUANTITY
NUMBER
Y Connector EAB021A 3 1
Y Connector EAB026A 2.5 1
T410 Connector EAB033A 1.6 1
T220 Connector EAB035A 2.3 8
0.040" TYGON tubing EAE005A 1.02 10 1
page 2/8
PART
DESIGNATION DIAMETER LENGTH QUANTITY
NUMBER
Sleevings GAL098A 25
Tube shielding XBA403A 1
page 3/8
1.2. Hydropneumatic connections
CIRCUIT FROM F.S. DIAM. LENGTH T.S. TO
AIR Atmosphere 2.05 140 EV13_2

EV13_1 1.52 70 Counting Syringe_1
Atmosphere 2.05 100 EV15_1
EV15_3 1.52 400 Diluent Tanker_2
Rinse_2 chamber
2.05 300 EV20_2
(Atmo.)
EV20_1 1.52 90 Draining Syringe_1
DILUENT Diluent Input 2.05 170 EV25_2

EV25_1 2.05 140 Diluent Tanker_4
2.05 65 Diluent Tanker_1
Diluent Tanker_1 2.05 350 EV3_1
EV3_3 S 2.05 120 Injector Syringe_2
EV3_2 S 1.52 50 S EV2_3
EV2_1 S 1.52 50 S EV1_3
EV1_1 S 1.52 200 S Isolator_1
Isolator_2 S 1.52 100 T1_1
T1_2 1.14 35 LMNE flowcell_4
T1_2 xba403a LMNE flowcell_4
T1_3 1.14 35 LMNE flowcell_2
T1_3 xba403a LMNE flowcell_2
EV1_2 1.52 250 T4_3
EV2_2 1.02 175 Injector Syringe_4
Injector Syringe_1 1.02 205 LMNE flowcell_5
LMNE flowcell_7 1.02 10 (Cap)
2.05 65 Diluent Tanker_3
Diluent Tanker_3 2.05 480 EV9_1
EV9_3 2.05 120 Diluter Syringe_4
EV9_2 1.52 50 EV10_3
EV10_1 1.52 15 T3_2
T3_1 1.52 640 EV18_3
EV18_1 1.52 330 Probe Rinsing Body_1
Probe Rinsing Body_2 1.52 300 EV17_1
EV17_2 1.52 820 T5_2
EV18_2 1.02 110 Sampling Syringe_2
EV10_2 1.52 380 EV21_3
EV21_1 1.52 50
EV22_1 1.52 200 S Reagent Heater_7
Reagent Heater_8 S 1.52 120 RBC chamber_1
Reagent Heater_2 S 1.52 120 DIL1/HGB chamber_1
Baso/WBC
EV21_2 1.52 220
chamber_4
page 4/8
ABX CLEANER Abx Cleaner Bottle 2.05 400 EV7_1

EV7_2 1.52 230 EV12_3
Baso/WBC
EV12_1 1.52 350
chamber_1
EV12_2 1.52 280 T3_3
ABX
Abx Eosinofix Bottle 2.05 400 EV8_1
EOSINOFIX
Reagent Heater_4 S 1.52 120 LMNE chamber_3
ABX
Abx Basolyse II Bottle 2.05 400 EV11_1
BASOLYSE II
Reagent Heater_9 S 1.52 60 S Reagent Heater_11
BASO/WBC
Reagent Heater_12 S 1.52 120
chamber_2
ABX
Abx Alphalyse Bottle 1.52 290 EV6_1
ALPHALYSE
EV6_2 1.52 480 DIL1/HGB chamber_2
SAMPLING Probe_1 1.02 205 Sampling Syringe_1
LMNE
LMNE chamber_4 1.30 20 M1_2 photocell
COUNTING
M1_1 photocell 1.30 320 EV4_1
EV4_2 1.02 130 T2_2
T2_3 1.02 240 Injector Syringe_5
Injector Syringe_3 1.02 95 EV5_1
EV5_2 2.05 420 T6_2
T2_1 S 1.85 11 LMNE flowcell_6
0.19 4 LMNE flowcell_6
LMNE flowcell_Output 1.52 20 E1_1 Anode fitting
E1_2 Anode fitting 1.52 70 Isolator_1
Isolator_2 1.52 80 E2_1 Ground fitting
E2_2 Ground fitting 1.52 335 EV24_2
EV24_1 1.52 120 T4_2
T4_1 1.52 160 S Reagent Heater_5
Reagent Heater_6 S 1.52 120 LMNE chamber_2
page 5/8
WBC/RBC BASO/WBC
1.52 200 EV23_3
COUNTING chamber_3
EV23_1 1.52 200 RBC chamber_3
RBC chamber_2 1.52 430 EV14_2
DRAININGS Rinse chamber_3 2.05 100 EV27_2

EV27_1 2.05 30 T7_3
DIL1/HGB chamber_3 2.05 70 EV28_2
EV28_1 2.05 20 T7_2
T7_1 2.05 20 T8_3
LMNE chamber_5 2.05 70 EV29_2
EV29_1 2.05 20 T8_2
T8_1 2.05 20 T9_3
RBC chamber_4 2.05 60 EV30_2
EV30_1 2.05 20 T9_2
T9_1 2.05 20 T10_2
BASO/WBC
2.05 55 EV31_2
chamber_5
EV31_1 2.05 20 T10_1
T10_3 2.05 30 T6_3
T6_1 2.05 20 T5_3
T5_1 2.05 40 M2_1 photocell
M2_2 photocell 2.05 20 Isolator_1
Isolator_2 2.05 280 EV26_1
EV26_3 S 2.54 150 S Draining Syringe_2
EV26_2 S 2.54 130 T11_3
Counting Syringe_5 2.05 125 EV16_1
EV16_2 S 2.54 340 T11_2
T11_1 2.54 20 Waste Output
NOTE Read this table as follows in this example :

The Liquid valve 7_2 corresponds to the ouput 2 of the valve number 7 (see
attached pneumatic diagram).
page 6/8
1.3. Functions of valves
Valve blocks are located close to the elements concerned.
Five different blocks:
Valves 1 to 11:
On the left side,
Horizontal block,
On the top of the reagent and injection
syringes.
Valves 12 to 16:
On the left side,
Vertical block,
Beside the counting syringe.
Valves 17 to 19:
On the right side,
Horizontal block,
On the sample carriage.
Valves 20 to 26:
On the right side,
Vertical block,
Beside the draining syringe.
Valves 27 to 31:
On the right side,
Horizontal block,
On top of the blending and count
chambers.
page 7/8
The following table shows the functions of each valve in the Pentra 60:
VALVE
VALVE SUBJECT VALVE FUNCTIONS
NUMBER
1 DIL LMNE Directing optical chamber / LMNE chamber
2 DIL LMNE Directing Internal / External sleeving
3 DIL LMNE Control Entry / Exit syringe
4 LMNE Transfer control LMNE tray /Injection syringe
5 LMNE Transfer control LMNE tray /Injection syringe
6 HEMOLYSE Control Entry / Exit syringe
7 CLEANER Control Entry / Exit syringe
8 EOSINOFIX Control Entry / Exit syringe
9 DIL Control Entry / Exit syringe
10 DIL Directing Circuit chamber / Needle circuit
11 BASOLYSE II Control Entry / Exit syringe
Directing WBC/BASO chamber / Needle
12 CLEANER
circuit
13 COUNTING SYRINGE Set in the atmosphere
14 COUNTING SYRINGE RBC/PLT count
15 TANK Set in the atmosphere
16 COUNTING SYRINGE Draining
17 NEEDLE Flushing out of rinsing unit
18 NEEDLE Commutation Diluent interior/exterior Needle
19 NEEDLE Reserved
20 DRAINING SYRINGE Set in the atmosphere
21 DIL Directing head for WBC count / Valve 22
22 DIL Directing diluent chamber dil.1
23 COUNTING SYRINGE WBC/RBC selection count
24 LMNE Exit from the optical chamber
25 DILUENT TANK Entry of the diluent
26 FLUSHING SYRINGE Flushing of the syringe
27 CHAMBER GROUP Drain rinse chamber
28 CHAMBER GROUP Drain first dilution chamber
29 CHAMBER GROUP Drain LMNE chamber
30 CHAMBER GROUP Drain RBC/PLT chamber
31 CHAMBER GROUP Drain WBC/BASO chamber
2. PNEUMATIC DIAGRAMS
See Pneumatic diagram on next page
page 8/8
ELECTRIC&ELECTRONIC
PRINCIPLES
1. Pentra 60 Main Board ............................................................. 2

1.1. Main board general view ................................................................. 2
1.2. Main board Test Points ................................................................... 3
1.3. Main board Potentiometers ............................................................. 5
1.4. Main Board configuration ................................................................ 6
2. Pentra 60 Boards ..................................................................... 7

2.1. Optical preampli board XAA423B .................................................... 7
2.2. LCD & Keyboard board XAA426B ................................................... 8
2.3. Motor relay board XAA427B ............................................................ 9
2.4. Carriage board XAA428B ................................................................. 10
2.5. LED board XAA429B ....................................................................... 11
3. Connections ............................................................................ 12
3.1. Chamber Heating Connector XBA386A ............................................ 12
3.2. Reagent Heating Coil Connector XBA387A ...................................... 13
3.3. Horizontal Carriage Motor XBA391A ................................................. 14
3.4. Upper Fan XBA393A ....................................................................... 15
3.5. IR sensor XBA394AS ....................................................................... 16
3.6. IR sensor XBA395AS ....................................................................... 17
3.7. IR sensor XBA396AS ....................................................................... 18
3.8. IR sensor XBA397AS ....................................................................... 19
3.9. RBC / WBC coaxe XBA398A ........................................................... 20
3.10. LMNE flowcell coaxe XEA663A (XBA399B+XBA490A) ................... 21
3.11. Barcode reader cable XBA402AS .................................................. 22
3.12. Diluent level sensor XDA605AS ..................................................... 23
4. Pentra 60 Synoptic .................................................................. 24

3. Electric & Electronic principles RAA016B
1. Pentra 60 Main Board
1.1. Main board general view
page 2/24
1.2. Main board Test Points
TEST POTENTI-
DESIGNATION TARGET TOLERANCE REMARQUE
POINT OMETRE
TP1 HB Gain adjustment R248 4.7V +/- 0.05V
TP2 PLT Threshold adjustment R159 300mV +/- 5mV
TP3 LMNE CIS Threshold adjustment R160 650mV +/- 5mV
TP4 LMNE OD Threshold adjustment R161 350mV +/- 5mV
TP5 Draining syringe motor R149 4.8V +/- 0.05V
TP6 Counting syringe motor R150 4.8V +/- 0.05V
TP7 Dilutor syringe motor R151 4V +/- 0.05V
TP8 Injection syringe motor R152 3V +/- 0.05V
TP9 UNUSED
TP10 Horizontal carriage motor R154 3V +/- 0.05V
TP11 Sample syringe motor R155 2V +/- 0.05V
TP12 Needle carriage motor R156 4.5V +/- 0.05V
TP13 RBC Threshold adjustment R158 300mV +/- 5mV
TP14 BASO Threshold adjustment R157 300mV +/- 5mV
TP15 BASO Comparator
TP16 RBC Comparator
TP17 PLT Comparator
TP18 LMNE CIS Comparator
TP19 LMNE OD Comparator
TP20 RBC line height adjsutment R133 XX V +/- 50mV Factory Adjusted
TP21 BASO gain adjustment R134 XX V +/- 50mV Factory Adjusted
TP22 PLT line height adjustment R135 XX V +/- 50mV Factory Adjusted
TP23 LMNE Resistive gain adjustment R136 XX V +/- 50mV Factory Adjusted
TP24 LMNE Optical gain adjustment R148 XX V +/- 50mV Factory Adjusted
TP25 RBC line width adjustment
TP26 RBC line reject adjustment R142 10µs +/- 0.5µs Factory Adjusted
TP27 RBC line pulse adjustment
TP28 BASO line width adjustment
TP29 BASO line reject adjustment R139 10µs +/- 0.5µs Factory Adjusted
TP30 BASO line pulse adjustment
TP31 PLT line width adjustment
TP32 PLT line reject adjustment R144 10µs +/- 0.5µs Factory Adjusted
TP33 PLT line pulse adjustment
TP34 LMNE Resistive line width adjustment
TP35 LMNE Resistive line reject adjustment R145 15µs +/- 0.5µs Factory Adjusted
TP36 LMNE Resistive line pulse adjustment
TP37 LMNE Optical line width adjustment
TP38 LMNE Optical line reject adjustment R147 5µs +/- 0.5µs Factory Adjusted
TP39 LMNE Optical line pulse adjustment
page 3/24
TP40 5V Power supply 5V

TP43 -12V Power supply -12V
TP44 GROUND
TP45 GROUND
TP46 GROUND
TP47 GROUND
TP48 Draining sensor R286 <1V With water
TP49 UNUSED
TP50 Reagent heating system CTN voltage
Thermostated room heating sytem
TP51
CTN voltage
TP52 LMNE Draining sensor R287 <1V With water
TP53 LMNE Lamp R11 6V
TP54 GROUND
TP55 GROUND
TP56 GROUND
TP57 GROUND
TP58 GROUND
TP59 GROUND
LMNE Resistive line reject2
TP60 R409 50µs +/- 2µs Factory Adjusted
adjustment
LMNE Resistive line reject2
TP61 R410 250µs +/- 5µs Factory Adjusted
adjustment
page 4/24
1.3. Main board Potentiometers
POTENTIO- TEST
DESIGNATION TARGET TOLERANCE REMARQUE
METRE POINT
R133 RBC line height adjsutment TP20 Factory Adjusted
R134 BASO gain adjustment TP21 Factory Adjusted
R135 PLT line height adjustment TP22 Factory Adjusted
R136 LMNE Resistive gain adjustment TP23 Factory Adjusted
R139 BASO line reject adjustment TP29 Factory Adjusted
R142 RBC line reject adjustment TP26 Factory Adjusted
R144 PLT line reject adjustment TP32 Factory Adjusted
R145 LMNE Resistive line reject adjustment TP35 Factory Adjusted
R147 LMNE Optical line reject adjustment TP38 Factory Adjusted
R148 LMNE Optical gain adjustment TP24 Factory Adjusted
R149 Draining syringe motor TP5 4.8V +/- 0.05V
R150 Counting syringe motor TP6 4.8V +/- 0.05V
R151 Dilutor syringe motor TP7 4V +/- 0.05V
R152 Injection syringe motor TP8 3V +/- 0.05V
R154 Horizontal carriage motor TP10 3V +/- 0.05V
R155 Sample syringe motor TP11 2V +/- 0.05V
R156 Needle carriage motor TP12 4.5V +/- 0.05V
R157 BASO Threshold adjustment TP14 300mV +/- 5mV
R158 RBC Threshold adjustment TP13 300mV +/- 5mV
R159 PLT Threshold adjustment TP2 300mV +/- 5mV
R160 LMNE CIS Threshold adjustment TP3 650mV +/- 5mV
R161 LMNE OD Threshold adjustment TP4 350mV +/- 5mV
R248 HB Gain adjustment TP1 4.7mV +/- 0.05V
page 5/24
1.4. Main Board configuration
DO
NO
TM
IFY
OD
D
O IFY
OTM
N
DO
NOTE If you have initialization’s problem with 68HC11 Microcontrolers, shut E20
jumper to reset Pentra 60 (instrument must be switched on).
page 6/24
2. Pentra 60 Boards
2.1. Optical preampli board XAA423B
OPTICAL PRE-AMPLI BOARD:
Perform an absorbance measurement

This is an Intensity / Tension convertissor
A photodiode measures absorbance of light through the cell, the optical pre-ampli board returns an
intensity proportionnal to the light signal (Light signal is proportionnal to the cell size)
page 7/24
2.2. LCD & Keyboard board XAA426B
LCD & KEYBOARD BOARD:
Includes Keyboard and LCD screen (Allows to have one flat connector for all connections)
LED board is connected to this board
page 8/24
2.3. Motor relay board XAA427B
MOTOR RELAY BOARD:
This an interconnection board where all the different motor and end of run sensor are connected
This board is connected to the main board with one flat cable
page 9/24
2.4. Carriage board XAA428B
CARRIAGE BOARD:
This an interconnection board where all the carriage electrical functions are connected
This board is connected to the main board with one flat cable
page 10/24
2.5. LED board XAA429B
LED BOARD:
This board support LEDs on the front panel. They give instrument state:
Red: instrument is busy
Green: instrument is ready to sample
page 11/24
3. Connections
3.1. Chamber Heating Connector XBA386A
page 12/24
3.2. Reagent Heating Coil Connector XBA387A
page 13/24
3.3. Horizontal Carriage Motor XBA391A
page 14/24
3.4. Upper Fan XBA393A
page 15/24
3.5. IR sensor XBA394AS
page 16/24
page 17/24
page 18/24
page 19/24
3.9. RBC / WBC coaxe XBA398A
page 20/24
3.10. LMNE flowcell coaxe XEA663A (XBA399B+XBA490A)
page 21/24
3.11. Barcode reader cable XBA402AS
page 22/24
3.12. Diluent level sensor XDA605AS
page 23/24
4. Pentra 60 Synoptic
See diagram next page.
page 24/24
ANALYSIS CYCLE TECHNOLOGY
1. Analysis cycle description .................................................. 2
2. Measuring principles .......................................................... 4

2.1. Multi Distribution Sampling System (MDSS) ................................. 4
2.2. RBC / PLT detection principles .................................................... 5
2.3. Hgb measurement ....................................................................... 6
2.4. Hct measurement ........................................................................ 6
2.5. RDW Calculation .......................................................................... 6
2.6. MCV, MCH, MCHC Calculation .................................................... 7
2.7. MPV Measurement ...................................................................... 7
2.8. Thrombocrit calculation ................................................................ 7
2.9. PDW calculation .......................................................................... 8
2.10. WBC count and differentiation ..................................................... 8
2.11. Dilutions summary ....................................................................... 12
4. Analysis cycle technology RAA016B
1. Analysis cycle description

• Aspiration of blood sample in excess (53µl for 5DIFF
mode)
• Translation of sampling carriage over Rinse chamber.
• External rinsing of needle and distribution of the first
volume of blood not used (3µl).
• Translation of sampling carriage over fisrt dilution

chamber, descent of the needle.
• Positioning the needle point into a tangential flux of
ABX Diluent (1.7ml) and synchronised distribution of
10µl of blood.
• Translation of sampling carriage over WBC/BASO count

chamber.
2ml of ABX BASOLYSE II and synchronised distribu-
tion of 10µl of blood.
Dilution
Blood Volume 10µl
BASOLYSE II Volume 2000µl
Dilution Rate 1/200
• Translation of sampling carriage over LMNE mixing

chamber.
1ml of ABX Eosinofix and synchronised distribution of
25µl of blood.
Dilution
Blood Volume 25µl
Eosinofix Volume 1000 µl
Diluent Volume 1000µl
Final Dilution Rate 1/80
page 2/12
• Translation of sampling carriage over Rinse chamber

• Distribution of the 5µl of residual blood during the
double rinsing (interior and exterior) of the needle.
• Translation of sampling carriage over first dilution

chamber.
• Sample of the 42.5µl of the first dilution.
• Rinsing of needle exterior (in the first dilution chamber)

with 0.4ml of diluent.
• Translation of sampling carriage over RBC and PLT

count chamber.
• Distribution of the 42.5µl of first dilution into a flux of 2
ml of diluent.
• Distribution of 0.5ml of diluent from the interior of the
needle.
Dilution
Initial Blood Volume 10 µl
Reagent Volume (RBC chamber) 2500 µl
Final Dilution Rate 1/10000
page 3/12
2. Measuring principles
2.1. Multi Distribution Sampling System (MDSS)
The ABX PENTRA 60 analysis cycle needs 3

blood specimens distributed as follows:
Diluent • One specimen for the first RBC/PLT dilution
and the measure of the HGB.
Air bubble
• Another specimen for the BASO/WBC count.
• The last specimen for the LMNE matrix.
Not used
LMNE Dilution These 3 specimens are provided by means of a

«multidistribution» principle:
WBC\BASO Dilution 53 µl of blood is aspirated inside the needle
(sufficient volume for all the dilutions) then frac-
RBC\PLT\HGB first dilution tions of this specimen is distributed to the
chambers with reagents.
Not used Extremities of the specimen are not delivered to
the dilutions.
The specimen distribution in the chambers

is carried out into a tangential flow of reagent
which allows a perfect mixing of the dilution
Needle
and prevents from viscosity problems (this
multi distribution in a reagent flow is ABX
patent).
Reagent
input
Tangential Flow Mix
Mixing chamber
BLOOD DISTRIBUTION IN A TANGENTIAL FLOW
page 4/12
2.2. RBC / PLT detection principles
Solution to be ananlyzed Impedance variation generated

by the passage of cells through
a calibrated micro aperture.
Vacuum = Constant
1 - The specimen is diluted in
an electrolytic diluent (current
conductor) and pulled through
the calibrated micro-aperture.
Volts
Two electrodes are placed on
each side of the aperture.
Analyzing
electronic
Electric current passes through
Electrodes
circuit the electrodes continuously.
2 - When the cell passes
through the aperture, electric
resistance between the two
Time electrodes increases
Pulse
proportionately with the cell
volume.
3 - The generated impulses have a very low voltage, which the amplification
circuit increases, so that the electronic system can analyze them and eliminate
the background noise.
Technical characteristics of the RED CELL and PLATELET count:

Dilution Measurement
Initial blood volume 10µl Method Impedance
Vol. ABX DILUENT 2500µl Ruby diameter 50 µm
Final dilution rate** 1/10000 Depression of count 200 mb
Reaction temperature 35°C Duration of the count 2x5 seconds.
** : Two successive dilutions are carried out :

Primary Dilution for RBC and PLT:
Blood (µl): 10
RBC
ABX DILUENT (µL): 1700 (dilution: 1/170)
Dilution RBC and PLT (from the primary dilution):
dilution (µl): 42.5
ABX DILUENT (µL): 2500 (dilution: 1/58.8)
Final dilution: (1/170)x(1/58.8)=1/10000
Results
Number of cells counted per volume unit x Calibration coefficient
Histograms
RBC: Distribution curves on 256 counting channels from 30fl to 300fl.
PLT: Distribution curves on 128 channels from 2fl to a mobile threshold. This
threshold moves according to the microcyte population present in the analysis
area.
page 5/12
2.3. Hgb measurement
The hemoglobin freed by the lysis of the red blood cells combines with the
potassium cyanide to form the chromogenous cyanmethemoglobin compound.
The compound is then measured by spectrophotometry, through the optical
part of the first dilution chamber, with a wave length of 550 nm.
Technical characteristics for the measurement of the hemoglobin
Volume of blood 10 µl Method Photometry
Volume ABX DILUENT 1700 µl Wavelength 550 nm
Volume ABX ALPHALYSE 400 µl
Complement ABX DILUENT 400 µl
Final dilution rate 1/250
Reaction temperature 35°C
Result
Final HGB result represents: Absorbance value obtained x Coefficient of
calibration
2.4. Hct measurement
The height of the impulse generated by the passage of a cell through the micro-
aperture is directly proportional to the volume of the analyzed RBC.
The hematocrit is measured as a function of the numeric integration of the MCV.
2.5. RDW Calculation
The study of the RBC distribution detects erythrocyte anomalies linked to

anisocytosis.
A Red Cell Distribution Width (RDW) will enable you to follow the evolution of
the width of the curve in relation to the cell number and average volume.
K SD
RDW =
MCV
With K = system constant

SD = Determined standard deviation according to statistical studies on
cell distribution.
MCV = Mean Corpuscular Volume of erythrocytes
page 6/12
2.6. MCV, MCH, MCHC Calculation
• MCV (Mean Cell Volume) is calculated directly from the RBC histogram.
• MCH (Mean Cell Hemoglobin) is calculated from the HGB value and the RBC
number.
The mean hemoglobin weight in each RBC is given by the formula:
HGB
MCH (pg) = x 10
RBC
• MCHC (Mean Corpuscular Hemoglobin Contained) is calculated according to

the HGB and HCT values. Mean HGB concentration in the total volume of RBC
is given by the formula :
HGB
MCHC (g/dL) = x 100
HCT
2.7. MPV Measurement
The MPV (Mean Platelet Volume) is directly derived from the analysis of the
platelet distribution curve.
2.8. Thrombocrit calculation
Thrombocrit is calculated according to the formula:
Plt (103/µl) x MPV (µm3)

PCT% =
10 000
page 7/12
2.9. PDW calculation
PDW (Platelet Distribution Width) is calculated from the

PLT histogram.
The PDW is represented by the width of the curve

between 15% of the number of platelets starting from 2 fl
(S1) and 15% of the number of platelets beginning with
the variable top threshold (S2).
S1 S2
PDW
2.10. WBC count and differentiation
2.10.1. General principles
The WBC count is carried out two times by two different sensors:
• In the BASO count chamber at the same time as the BASOS count.
• In the optical chamber during the acquisition of the LMNE matrix (available
from the V1.1 software version)
The reference count is the one obtained in the WBC and BASOS count chamber.
2.10.2. BASO / WBC Count
Detection principles is the same as for RBC.

Differentiation betwen BASOs and other leukocytes is obtained by means of
the new BASOLYSE II specific lysing action.
The basophils are located from threshold <2> to the end. 100 % of the leukocytes
are represented by the total number of nucleic particles plus the basophils from
the threshold <1> to <3>.
Percentage of basophils is calculated from the number of particles existing
from the threshold <2> to <3>.
BA1 BA2 BA3
WBC BASO
page 8/12
Technical Characteristics of the WBC/BASO count

Volume of blood 10µl Method Impedance
Volume of BASOLYSE II 2000µl Ruby diameter 80µm
Rate of dilution 1/200 Depression of count 200mb
Reaction temperature 35°C Duration of the count 2x6 seconds
Results
WBC: Number of cells per volume x Coefficient of calibration.
BASO: Number of cells per volume x Coefficient of calibration in percentage
regarding the number of counted cells (BASO + WBC nuclei)
2.10.3. LMNE matrix
The WBC matrix analysis of the ABX PENTRA 60 is based on 3 essential

principles:
1- The double hydrodynamic sleeving: «DHSS» (ABX patent)
2- The volume measurement : impedance changes.
3- The measurement of transmitted
2) SECOND FOCUSED FLOW FOR OPTICAL light with 0° angle, which permits a
DETECTION
response according to the internal
structure of each element and its
absorbance by means of incident
light diffusion.
25µl of whole blood is delivered to

the LMNE chamber in a flow of
EOSINOFIX. This reagent lyses the
RBC, stabilizes the WBC in their na-
tive forms and stains the eosinophil
nuclei with a specific coloration.
The solution is then stabilized with
diluent and transferred to the
measuring chamber. Each cell is
measured both in absorbance
(cytochemistry) and resistivity (vo-
lume).
1) PRIMARY FOCUSED FLOW FOR IMPEDANCE
MEASUREMENT
Technical characteristics of the WBC count during the acquisition of the matrix
Volume of blood 25 µl Method Impedance with hydrofocus
Volume Eosinofix 1000 µl Ruby diameter 60 µm
Volume diluent 1000 µl Diameter of the flow 42 µm
Rate of final dilution 1/80 Injection duration 12 seconds
Reaction temperature 35 ° C Volume injected 72 µl
Incubation duration 12 s.
page 9/12
Results
From these measurements, a matrix is drawn up with volumes on the X-axis
and optical transmission on the Y-axis
The study of the matrix image permits the clear differentiation of 4 out of 5
leukocytes populations. As a matter of fact, the basophil population is very
small compared to the other 5 in a small blood sample.
NEUTROPHILS EOSINOPHILS
ABSORBANCE
LMNE
LIC
VOLUME
LYMPHOCYTES ALY MONOCYTES
EOSINOPHILS: With reagent action on cytoplasmic membranes, the leukocytes

keep their native size and only eosinophils are colored for optical separation.
Eosinophils will be situated in the upper part of the optical Y-axis due to their
strong absorbance qualities and their size, which is nearly equivalent to large
neutrophils.
page 10/12
NEUTROPHILS: The neutrophils, with their cytoplasmic granules and their

generally segmented nuclei, will scatter light depending on their morphological
complexity. A hypersegmented neutrophil will give an increased optical response
with respect to a young neutrophil population which will be in the upper position
of the optical axis depending on the presence of segmentation and/or granules.
LYMPHOCYTES: The lymphocytes being small with regular shape, are

positioned in the lower part of both the optical axis and volume axis. Normal
lymphocyte populations are generally observed with a good volume
homogeneity. Large lymphocytes are detected in the ALY (Atypical Lymphocy-
tes) zone, where reactive lymphoid forms, stimulated lymphocytes and plas-
mocytes are also to be found. The far left side of the lymphocyte zone should
normally be empty, but when small lymphocytes are present, population may
exist in this area. The presence of platelet aggregates is detected by a distribu-
tion pattern that moves from the origin of the matrix (background zone) into the
lymphocyte zone. The NRBCs with their cytoplasmic membranes lysed like the
erythrocytes, will have their nuclei situated to the far left side of the lymphocyte
zone.
MONOCYTES: The monocytes, being cells with large kidney shaped nuclei
and a large non-granular cytoplasm, will neither be scattered nor absorb a large
amount of light. They will therefore be positioned in the lower part of the optical
axis but clearly to the right of the volume axis. Certain large monocytes can be
found on the right side of the matrix in the lower LIC (Large Immature Cells)
zone. The immature granulocytic cells are detected by their larger volumes and
by the presence of granules which increase the intensity of the scattered light.
Therefore, cells such as metamyelocytes will be found clearly to the right of the
neutrophils and nearly at the same level. Myelocytes and promyelocytes will
be found in saturation position on the far right of the matrix. These last three
populations will be counted as LIC (Large Immature Cells) and their given results
are included in the neutrophil value. The blast cells will be found generally to
the right of the monocytes, and, as such, will increase the LIC count. Small
blasts will be found between the normal lymphocytes and monocytes. Platelets
and debris from erythrocyte lysis represent the background noise population
located in the lower left area of the matrix. Most of the population partition
thresholds are fixed and give the limits of the morphological normality of
leukocytes. Changes in the morphology of a population will be expressed on
the matrix by a shifting of the corresponding population.
page 11/12
2.11. Dilutions summary
TECHNICAL BLOOD REAGENT FINAL REACTION

REAGENT
CHARACTERISTICS FOR VOLUME VOLUME DILUTION RATE TEMP.
WBC Count in WBC/BASO
10µl BASOLYSE II 2000µl 1/200 35°C
Chamber
WBC Count during acquistion EOSINOFIX 1000µl
25µl 1/80 35°C
of LMNE matrix DILUENT 1000µl
BASOPHIL Count 10µl BASOLYSE II 1000µl 1/200 35°C
RBC and PLT Count 10µl DILUENT 2500µl 1/10000 35°C
DILUENT 1700µl
Hemoglobin Measurement 10µl LYSE 400µl 1/250 35°C
DILUENT (Compl.) 400µl
page 12/12
SOFTWARE VERSIONS
1. Software Overview: User Menu Overview .......................... 2
2. Software Overview: Technician Menu Overview ................ 3
3. Software Release ............................................................... 4

5. Software versions RAA016B
1. Software Overview: User Menu Overview
page 2/4
2. Software Overview: Technician Menu Overview
page 3/4
3. Software Release
Software Pentra 60
Release Serial Number Note
First release,
V0.82 60 002 to 60 010 Not on field
More utilities, Default values changed,

V1.02 60 011 to 60 090 No change on cycle and Results reliability
New basophil flag management 1,

V1.04 60 091 to ... No change on cycle and Results reliability
V1.05 See Technical note RAH680A
V2.01 See Technical note RAH772A
V2.11 See Technical note RAH903B
V2.16 See Technical note RAN014A
page 4/4
OUTPUT FORMAT
See ABX Diagnostics output format for haematology analyzers P/n: RAA024
ALARMS & ERROR LIST
1. Printer ................................................................................. 2
2. Transmission ...................................................................... 2
3. Calibration .......................................................................... 2
4. Technical Operations .......................................................... 3
5. Reagents ............................................................................ 3
6. Temperature ........................................................................ 3
7. Alarms & Error list RAA016B
1. Printer
DISPLAYED MESSAGES CAUSES USER ACTIONS
The printer is disconnected, - Switch on or

Printout operations
switched off or has not been - press "ON LINE"or
disabled
selected - see the printer user’s manual
Defect on printer, make sure there is Printout operations - Feed paper or

paper disabled - see the printer user’s manual
2. Transmission
No ENQ character received on RS232
No ACK character received on RS232
Read error on RS232 Check the RS 232 configuration

Menu "SETUP / RS 232 / RS232
defect on
Write error RS232 CONFIGURATION"
transmission
operations
Timeout overflow on RS232 Call ABX representative service
department
Result transmission impossible
Transmission mode &/or format

incompatible
3. Calibration
Perform an autoconcentrated
Coefficients out of the
Coefficients outside the limits cleaning
ranges given section 3
Run another calibrator vial
Data not saved, value out of incoherent value entered

Re-type in the item
range by the operator
Incoherent date entered

Illegal date Re-type in the date
by the operator
Minimum tagged CBC incorrect, Selected results for

Select at least 3 results
at least 3 calibration calculation < 3
page 2/3
7. Alarms & Error list RAA016B
4. Technical Operations
Blocked motor
Emergency stop, Run an
Incorrect Drains control the motor operations : Menu
autocontrol
Thermal door opened "MAINTENANCE / MECHANICAL
SYSTEMS / CHECK MOTORS"
.....not reaching home Blocked motor
Thermal door opened open during a cycle close the door and rerun the cycle
Incoherent time entered

Illegal time Enter the correct time
by the operator
5. Reagents
DISPLAYED MESSAGES EFFECTS USER ACTIONS
replace the diluent container

No diluent, check level diluent reservoir empty (menu "REAGENTS/LEVEL-
CHANGE")
Replace the bottle (menu

Reagent low level (reagent name) none
"REAGENTS/LEVEL-CHANGE")
Message triggered at the control the reagent levels or

Reagent low level
end of the Startup /and replace it.
Call ABX representative Service

Drain time out Draining problems
Department
6. Temperature
Call ABX representative

Temperature out of range Thermic regulation problem
department
Operating temperature not

Heating coil initialization reached Wait for a few minutes
page 3/3
MAINTENANCE
1. Maintenance ...................................................................... 2
1.1. Introduction .................................................................................. 2
1.2. Daily customer maintenance ........................................................ 2
1.3. Weekly customer maintenance .................................................... 2
1.4. Maintenance table ....................................................................... 3
2. Maintenance kits ................................................................ 4

2.1. 6 month Maintenance kit .............................................................. 4
2.2. Yearly Maintenance kit ................................................................. 4
2.3. Pistons Maintenance kit ............................................................... 5
2.4. Fitting Kit ..................................................................................... 5
2.5. Screws Kit ................................................................................... 6
3. Procedures ......................................................................... 8
3.1. Procedure chart tables ................................................................. 9
3.2. Required tools and products ....................................................... 10
8. Maintenance RAA016B
1. Maintenance
1.1. Introduction
WARNING
Customer maintenance has to be carried out according to the recommended
frequency chart table and after having attended an ABX approved customer trai-
ning course.
The system warranty may be affected if damage occurs after a non trained technician
intervenes or if replaced spare parts and consumables do not come from an ABX
approved origin.
1.2. Daily customer maintenance
No special adjustments or maintenance has to be done on your equipment if

the recommended startup and shutdown procedures are explicitly respected.
See the ABX Pentra 60 User Manual for the daily rinsing and cleaning of the
system.
1.3. Weekly customer maintenance
An overall check for cleanliness of the system is recommended every week.

All traces of blood or reagent have to be wiped off as soon as possible using a
piece of cloth and distilled water.
CAUTION
Never use solvent or abrasive cleaning material to clean the system.
page 2/10
1.4. Maintenance table
The ABX Pentra 60 maintenance is scheduled on 3 different maintenance kits.

The maintenance schedule depends on the number of cycles per day, follow
the chart table to know the kit part number to use:
CYCLES PER DAY Õ <30 30 to 120 >120 KIT PART

NUMBER
MAINTENANCE EACH Õ 1Y 2Y 6M 1Y 18M 2Y 6M 1Y ×
Sampling probe O’ring X X X X X X X X

XEA485AS
Reagent syringe O’ring X X X X X X X X Procedure
RAS236
Draining and Counting syringes O’ring X X X X X X X X
Sampling syringe X X X X X X
5DIFF syringe X X X X X X
Draining chamber X X X X X X
XEA486BS
Optical bench lamp X X X X X X Procedure
RAS237
LMNE flowcell coaxial X X X X X X
Diluent reservoir joint X X X X X X
Counting heads X X X X X X
Sampling probe replacement X X X

XEA581BS
Reagent pistons replacement X X X
Procedure
Counting and Draining syringe pistons RAS238
X X X
replacement
page 3/10
2. Maintenance kits
2.1. 6 month Maintenance kit
Follow the RAS236 procedure for maintenance with the XEA485AS kit. The kit
is including:
SPARE PARTS KIT

Part Number Qty
XEA485AS
FAA053A Sampling Needle O’ring 1.40x1.25 Fluocarbon 1
FAA065A Reagent Syringe O’ring 6.30x2.40 Silicon 1
XDA621A Vacuum/Waste pump O’ring 30.80x3.80 + Wedge 2
XDA622A Reagent Syringe O’ring 15.54x2.62 + Wedge 4
XEA019A Silicon Grease 1
2.2. Yearly Maintenance kit
Follow the RAS237 procedure for maintenance with the XEA486BS kit. The kit
is including:
SPARE PARTS KIT

Part Number Qty
XEA486BS
DAJ007A Lamp 20W 9.5V 1
FAA040A 5DIFF Syringe O’ring 12.10x2.70 Silicon 1
FAA046A Coaxial cable O’ring 2.75x1.60 Viton 2
FAA064A Sampling Syringe O’ring 1.42x1.52 Fluocarbon 2
FAA066A Draining Chamber O’ring 13.10x1.60 Silicon 4
FAA067A 5DIFF Syringe O’ring 2.40x1.90 Fluocarbon 4
GBG275A Aperture Counting head EPO Joint 4
XEA663A LMNE flowcell coaxial 1
XEA286AS Waste chamber Joint + Washer 1
JAJ007A Polyeth. tape 1m50
page 4/10
2.3. Pistons Maintenance kit
Follow the RAS238 procedure for maintenance with the XEA581BS kit. The kit
is including:
PISTONS KIT
Part Number Qty
XEA581BS
GBC031A Syringe, Lyse piston 1
GBC030A Syringe, Reagent piston 4
GBG052A Syringe, Vacuum/Waste piston 2
GBG091A Carriage, Needle guide 1
XDA619AS Needle, Sampling needle 1
2.4. Fitting Kit
The fitting kit contains the most common pneumatic and hydraulic parts:
FITTING KIT
Part Number Qty
XEA311A
EAA005A Straight connector Ø1.6mm 1/8 10
EAA006A Straight connector 10
EAA009A Bent connector I=1.5 10/32 10
EAB002A "L" Connector 10
EAB003A Connector Ø4mm H19.5 20
EAB005A "T" Connector Ø1.6mm T10-6 30
EAB006A "T" Connector Ø2.3mm 30
EAB009A Straight connector Ø1.6mm 30
EAB010A Straight connector Ø2.3mm 30
EAA013A Bent connector 1/8 10
EAA014A Straight connector 1/8 10
EAB026A "Y" Connector Ø2.5mm 3
EAB021A "Y" Connector Ø3mm 3
EAB035A "T" Connector Ø2.3mm T220-6 10
EAB014A Straight connector "Y" Ø1.5mm/1.5mm 10
page 5/10
2.5. Screws Kit
The screws kit contains the most common screws used in Pentra 60:
SCREWS KIT
Part Number Qty
XEA293AS
KAA002A CHC M3x6 Screw 20
KAB002A FHC M3x6 Screw 20
KAC002A TC M3x5 Screw 20
KAE003A BHC M4x12 Screw 20
KAH001A HU M3 Nut 20
KAH024A Nut 1/4-28 (US Std) 10
page 6/10
KAJ001A M Washer Diam.3 20
KAJ007A AZ Washer Diam.3 20
KAJ010A W Grower Washer Diam.5 20
KAM002A Plastic Crosspiece M3x6 Lg.8 10
KAM004A Plastic Crosspiece M3.5x6 Lg.6 10
KAM011A Plastic Crosspiece M3.5x6 Lg.5.7 10
KAM017A Brass Crosspiece M3.5x6 Lg.3 10
KAB016A FX M3x8 Screw 5
page 7/10
3. Procedures
Maintenance and adjustments that need to be done on ABX Pentra 60 are

NOTE divided into «procedures» according to concerned assemblies. This should
make any update easier as all interventions can be done with the corresponding
«procedure» on its own.
CONCERNING PARTS:
- Hydraulic maintenance and adjustments.
- Pneumatic maintenance and adjustments.
- Electrical maintenance and adjustments.
- Power supply maintenance and adjustments.
- Electronic maintenance and adjustments.
- Printer maintenance and setup.
NOTE Each procedure has to be read entirely before beginning the intervention:
RED Procedures are Adjustment procedure
BLEU Procedures are Maintenance procedure
GREEN Procedures are Dismantling procedure
WARNING
When cleaning instruments, disposable gloves should be worn.
page 8/10
3.1. Procedure chart tables
P/N PROCEDURE CONCERNS
Unpacking - Working conditions - General description - Printer &

RAS235 INSTALLATION
instrument connection - Reagent connections - Priming & Startup
Sampling Probe: O’ring Replacement - Reagents syringe: O’rings

RAS236 6 MONTH MAINTENANCE
Replacement - Vacuum/Waste pump: O’rings Replacement
Sampling syringe: O’ring Replacement - 5DIFF syringe: O’ring

Replacement - Draining chamber: O’ring Replacement - Optical Bench:
RAS237 YEARLY MAINTENANCE Lamp & LMNE flowcell coaxial Replacement - Diluent reservoir: Joint
and washer Replacement - Counting Heads: RBC/WBC Coaxial, O’ring
and Aperture joint Replacement
EVERY 2 YEARS MAINTENANCE Sampling probe: Probe & Guide Replacement - Reagents syringe:
RAS238
KIT Pistons Replacement - Vacuum/Waste pump: Piston Replacement
RAS239 DECONTAMINATION & RINSE Instrument Decontamination & Rinse
LMNE FLOWCELL
RAS240 Optical Bench Check and Adjustment
ADJUSTMENT
RAS241 CHAMBERS ADJUSTMENT Alignment Check and Adjustment of chambers assembly
Probe Home position Check and Adjustment - Probe position Check

RAS242 PROBE ADJUSTMENT
and Adjustment
HGB Blank Adjustment - Aperture Current Check - RBC/PLT Gain

Adjustment - WBC/BASO Gain Adjustment - Drain sensor Adjustment -
RAS243 MAIN BOARD ADJUSTMENT
LMNE sensor Adjustment - Motor Current Adjustment - Thresholds
Adjustment
Reagent heating system temperature Check - Reagent heating system

RAS244 THERMIC ADJUSTMENT temperature Adjustment - Thermostatedcompartment temperature
Check - Thermostated compartment temperature Adjustment
Draining syringe vacuum Check - Counting syringe vacuum Check -

RAS245 VACUUM ADJUSTMENT
Counting syringe vacuum Adjustment
RAS247 FRONT PANEL DISMANTLING Front panel Dismantling - Front panel Mounting
RAS248 HEATER ASSY REPLACEMENT Heater Assy Dismantling - Heater Assy Mounting
RAS249 BUBBLING ADJUSTMENT Bubbling Adjustment Menu presentation
POWER SUPPLY
RAS250 Power supply Dismantling & Replacement
REPLACEMENT
START CYCLE MICROSWITCH Start cycle microswitch Dismantling - Start cycle microswitch
RAS251
REPLACEMENT Mounting
CHECK UP AFTER
RAS252 Instrument check up: Repeatability - Calibration
INTERVENTION
Cycle start condition - Count and Measurement of the WBC group -

STEP BY STEP ANALYSIS
RAS253 RBC/PLT group count - Filling of Tank - Rinsing of the RBC/PLT count
CYCLE CHECK
chamber
OPTICAL BENCH DISMANTLING Optical bench Dismantling - Optical bench Replacement - Emission
RAS254
& REPLCEMENT gun location Control - Optical bench lamp alignement Control
BARCODE READER
RAS255 Instrument configuration for Barcode Reader
CONFIGURATION
RAS304 LMNE BALANCE ADJUSTMENT Adjsutment of the LMNE balance
page 9/10
3.2. Required tools and products
T O O L S P R O D U C T S
DESIGNATION PART DESIGNATION PART

NUMBER NUMBER
HEXAGONAL EMPTY SAMPLE

KEYS TUBES
DYNAMOMETRIC MAG019A SILICONE GREASE LAM004A

SCREW DRIVER
A302
DYNAMOMETRIC MAG020A GREASE FOR XEA381AS

SCREW DRIVER MECHANICAL
A301 ASSEMBLIES
DYNAMOMETRIC MAG013A SOFT TISSUE

SCREW DRIVER
A300
CLAMPS LIQUID SOAP
SCALPEL DISTILLED WATER
CUTTING PLIERS MICROPIPETTE TIP
PAIR OF FLAT PIECE OF

SCISORS STIFF PLASTIC
VOLTMETER SYRINGE 5ML
FLAT LATEX RBC LAD002AS

SCREWDRIVER
BARFLEX FELT PEN
THERMOMETER
TORX KEYS
page 10/10
INSTALLATION
• CONCERNS
1 - Working conditions
2 - Unpacking
3 - General description
4 - Installation
5 - LX300+ Printer connection
6 - Reagent connections
7 - Priming and startup
• REQUIRED TOOLS
Hexagonal keys
Pentra 60
• REQUIRED PRODUCTS
Pentra 60 Reagents:
Bottles,
Waste container.
• INTERVENTION TIME
60 minutes
• FREQUENCY
• SPECIFIC KIT OR CONSUMABLES
Installation kit: XEA791A
RAS235B
INSTALLATION
WARNING
Disposal gloves and white coat must be worn by the operator.
Local or national regulations must be applied in all the operations.
INSTALLATION KIT
Part Number Qty
XEA791A
DAJ007A OPTICAL,LAMP OPTIC BENCH P60 1

DBH001A COLLAR,TYRAP SMALL 3
DBH002A COLLAR,TYRAP BIG 3
DBK003A ADHESIVE,HOLDER FOR FLAT CABLE 1
EAB021A FITTING,Y D=3MM 1
EAB033A FITTING,T 1.6MM T410-6 1
EAB035A FITTING,T D=2,3MM 1
EAC010A FITTING,LUER FEMALE I=3MM 2
Pentra 60
EAC019A FITTING,LUER MALE I=3MM 1

EAE005A TUBING,TYGON 1,016(0,040) 2 Meters
EAE011A TUBING,CRYSTAL 3X6 2 Meters
EAE028A TUBING,CRYSTAL 4X6 2 Meters
EAE034A TUBING,TYGON 2.54(0,100) 2 Meters
FAA053A O’RING,SAMPL. NEEDLE MICROS OT 1
FBH016A COVER,DUST COVER PENTRA60 1
FBL001A REAGENT,REAGENT CAP 2 HOLES 2
GAK302A REAGENT,BOTTLE STOPPER D=40 3
GBG138A DOOR,KEY FOR T° ROOM P60 1
GBG145A REAGENT,STRAW D=20 P60 1
GBG155A REAGENT,BOTTLE STOPPER D=25 1
GBG156A O'RING,APERTURE P60/M60 2
GBG245A REAGENT,STRAW D=28 P60/ACT 3
JAA011A PACKAGING,CARDBOARD BOX 1
MAB002A TOOL,ALEN KEY 2,5MM 1
MAB018A TOOL,ALEN KEY 3MM 1
MAB090A TOOL,TORX KEY T10 1
XDA483B VALVE,LIQ. 3WAYS W/O COIL 1
XDA619AS NEEDLE,SAMPLING NEEDLE P60 1
XDA621A O'RING,VAC/WASTE PUMP + WASHER 1
XDA692A TUBING,DILUENT ASPIRATION P60 1
XDA693A TUBING,WASTE P60 1
XEA018A REAGENT,STRAW DILUENT L=360 1
XEA019A GREASE,SILICON CONTAINER 1
p.2/11
RAS235B
WORKING CONDITIONS
1 - Environment
The ABX Pentra 60 should be operated in an indoor location only. Operation at an altitude over
2000 meters (6000 Feet) is not recommended. Instrument is designed to be safe for transient
voltages according to INSTALLATION CATEGORY II and POLLUTION DEGREE 2.
Please ask your ABX representative service center for any information about operating loca-
tion when it does not comply with the recommended specifications.
2 - Location
The ABX Pentra 60 should be placed on a clean and level table or work station. Please note
that the ABX Pentra 60, printer and reagents weigh approximately 40 kilograms (88 lbs). Avoid
exposure to sunlight. Proper ventilation requires that a space of at least 20 cm (8 inches) must
be left behind the apparatus.
INSTALLATION
3 - Grounding
Proper grounding is required. Check that the wall ground (earth) plug is correctly connected to
the laboratory grounding electricity installation. If there is no ground then use a ground stake.
Current electricity Standards must be applied.
4 - Humidity and temperature conditions
The ABX PENTRA 60 must function between 16 to 34°C (61 to 93°F). Maximum relative humidity
80% for temperatures up to 31°C (88°F) decreasing linearly to 50% relative humidity at 40°C
(104°F). If it is kept at a temperature less than 10°C (50°F), the instrument should be allowed to
sit for an hour at the correct room temperature before use.
5 - Electromagnetic environment check
The ABX Pentra 60 has been designed to produce less than the required level of electromagnetic
interferences in order to operate in conformity to its destination. The electromagnetic interferences
by the ABX Pentra 60 are limited to a level allowing the correct operation of other instruments in
conformity with their destination.
In case of problems, check that the instrument is not placed in the proximity of electromagnetic
fields, or short wave emissions (radars, X-rays, scanner, etc...).
6 - Environment protection
When old the instruments and their accessories must be collected by a laboratory specialized
in elimination and recycling of this kind of equipment according to the legislation.
p.3/11
INSTALLATION
GENERAL DESCRIPTION
1. Cover:
Before any attempt to remove the cover, open the
pneumatic access door.
2. Pneumatic access door:
allows the operator to access hydraulic parts for mainte-
nance operations. It is mandatory to keep the door
locked during the measuring cycles as it ensures the
heating of the dilutions !
3. Sampling probe:
This needle is used for the 53µl whole blood sampling.
4. Control panel:
This panel allows the operator to communicate with the
instrument :
- To access the different cycles
- To identify the patients
- To setup the instrument, etc...
5. Door to reagents bottles:
Pentra 60
Access to the reagent bottles in order to replace them

Diag.1 when empty.
Turn the locker as shown on the Diag. 2 to open the pneumatic protection door.
Diag.3
Diag.2
1 - Mechanical and Hydraulic modules (Diag.4&5)
1. Sampling carriage:
• Ensuring the needle positioning for the different sampling stages and the distribution.
• Support the sampling syringe and the distribution of the blood
2. Sampling syringe:
• Take the specimen and distribute the portions into the different chambers.
• Take the sample from the first dilution and distribute into the RBC/PLT chamber.
3. Count assembly:
• Receives the different rinsing and dilutions.
• Ensure the temperature control of the dilutions.
• Ensure the counts for WBC, BASOS, RBC, PLT, Hct, Hb.
p.4/11
RAS235B
INSTALLATION
Diag.4&5
4. Draining syringe:
• Draining of the different chambers.
• Bubbling of the mixtures.
• Transfer, by vacuum, the LMNE specimen of the mixing chamber towards the injector of the
optical chamber.
5. Diluent tank:
• To hold the necessary diluent for an analysis cycle.
• To avoid the risk of the «degassing» of the diluent as it is being aspirated by the syringes.
This tank is vacuum filled by the counting syringe.
6. Counting syringe:
• Ensure the vacuum for the WBC and the BASOS counts.
• Ensure the vacuum for the RBC and the PLT counts.
• Ensure the vacuum for the filling of the internal tank with diluent.
7. Syringe assembly for the LMNE Matrix:

• Ensure the correct proportioning of the stop diluent in the LMNE preparation chamber
• Injection of the specimen into the optical chamber
• Injection of the interior and exterior gains into the optical chamber.
8. Syringe assembly for the reagent distribution:

• Ensure the correct distribution of the different reagents.
• Lysing agent for hemoglobin (ABX ALPHALYSE)
• Cleaning reagent (ABX CLEANER)
• Lysing agent LMNE (ABX EOSINOFIX)
• Diluent used for the dilutions (ABX DILUENT)
except the LMNE stop diluent
• Lysing agent for WBC/BASO (ABX BASOLYSE ll)
9. Optical bench:
• Ensure the support and the adjustment of the optical chamber
• Ensure the support and the adjustment of the lamp
• Ensure the support and the adjustment of the optical and electronic elements.
p.5/11
INSTALLATION
2 - Rear panel
Internal left
1 -hand
Wasteside,
output
2 - Diluent input
Mother board
3 - Serial number label
4 - Barcode reader connector
5 - Printer connector
6 - RS 232 C Output
7 - Power supply cable
Pentra 60
Diag.6
INSTALLATION
- Turn the locker (as shown on the Diag.2) to open the pneumatic protection door.
- Free the probe carriage removing both plastic blockers (Diag.7).
Diag.7
- Connect the power suplly cable (Diag.6 rep.7).
p.6/11
RAS235B
1 - Reagent connections
Use Luer connectors delivered in the Installation kit to connect Reagents.
- Bottle & Container connections:

1 - ABX ALPHALYSE
2 - ABX BASOLYSE II
3 - ABX EOSINOFIX
4 - ABX CLEANER
5 - ABX DILUENT
6 - WASTE CONTAINER
2 - Diluent & Waste containers connection
- Remove the diluent straw.
INSTALLATION
- Install the straw deep in the container.
IMPORTANT
Diluent Tube:
Use 3x6 cristal tube for diluent connection. Tube length must be under 2 meters
Waste Tube:
Use 4x6 cristal tube for waste connection. Tube length must be under 2 meters
Diag.8
p.7/11
INSTALLATION
3 - Bottle connection
- Open reagent bottles and replace the caps by the «bottle stoppper and reagent straw» assembly.
- Open the reagent front door.
- Put the reagent botlles in the compartment.

Pentra 60
Diag.9 Diag.10
IMPORTANT
After the priming of one or several reagents, close the pneumatic access door (Right side
of the instrument), switch off the PENTRA 60, then re-start it
LX 300+ PRINTER CONNECTION
Setting up the printer:
- Remove all the package protections
- Install the paper-feed knob
Diag.11
p.8/11
RAS235B
INSTALLATION
Diag.12&13
Open the ink ribbon access door at the top of the printer and install the ribbon as shown in Diag
14 & 15:
- Slide the printer head to the middle of the printer.
- Insert the ribbon cartridge into the printer
- Guide the ribbon between the print head and ribbon guide.
- Slide the printer head from side to side to make sure it moves smoothly.
Diag.15
Diag.14
p.9/11
INSTALLATION
- Install the paper supports (Diag.16) for single sheets paper use only.
Diag.16
- The printer is connected to the ABX Pentra 60 with the cable delivered with the instrument.
Lock the connector in place by tightening the 2 screws on each end of the connector to the ABX
Pentra 60. Attach the other end of the cable to the printer and lock the printer connector in place
Pentra 60
by the means of the 2 clips located on the connector itself (Diag.17).
Diag.17
- For continuous paper, introduce it in the slot at the back of the printer and use the sprocket
covers to load paper, or feeding the paper frontward when using single sheets (see printer user
manual).
Diag.19
Diag.18
p.10/11
RAS235B
PRIMING & STARTUP
Instrument priming
- Open the reagent «LEVEL/CHANGE» menu.

REAGENTS\LEVEL/CHANGE 16:05
DILUENT
0% CHANGE
EOSINOFIX 1 - LEVEL/CHANGE
11% CHANGE
BASOLYSE II
23% CHANGE
CLEANER
20% CHANGE
HGB LYSE
52% CHANGE
- Use the and to display the reagent
INSTALLATION
REAGENTS\LEVEL/CHANGE 16:05 «CHANGE» key in reverse video.
DILUENT
100% CHANGE
EOSINOFIX 1 - LEVEL/CHANGE - Press : a prime cycle is automatically run.
99% CHANGE
BASOLYSE II
98% CHANGE - The reagent level is updated to «100%»
CLEANER
98% CHANGE
ALPHALYSE
98% CHANGE
Never pour reagent from one container to another. Particles present in the bottom of the old
container can contaminate the new reagent, causing unacceptable background counts, especially
for PLTs.
IMPORTANT
Before analyzing samples, visually inspect reagent lines and pumps for air bubbles. Repeat
priming if air bubbles are still present. Call the ABX representative service department if
priming does not eliminate air bubbles.
Never initiate two Lyse prime cycles back-to-back. This causes excessive foaming in the
waste chamber. Run a blank cycle between each Lyse prime cycle.
- Run a STARTUP cycle and follows the ABX Pentra 60 User Manual's instructions.
p.11/11
6 MONTH MAINTENANCE
• CONCERNS
Instrument maintenance step by step
• REQUIRED TOOLS
Hexagonal keys
Dynamometric screw driver A302, A301, A300
Cutting pliers
Flat screw driver
Philips screw driver
Torx keys
Pentra 60
Minoclair
2h
• FREQUENCY
See Maintenance table

Spare parts kit: XEA485AS
RAS236B
6 MONTH MAINTENANCE
SAMPLING PROBE
REAGENTS SYRINGE
DRAINING & COUNTING SYRINGES
INSTRUMENT CLEANING
WARNING
6 MONTH MAINTENANCE KIT

Part SPARE PARTS KIT XEA485AS Qty
Number
FAA053A O’ring 1.40x1.25 Fluocarbon 1
Pentra 60
Sampling needle
FAA065A O’ring 6.30x2.40 Silicon 1
Reagent syringe
XDA621A O’ring 30.80x3.80 + wedge 2
Vacuum/Waste pump
XDA622A O’ring 15.54x2.62 + wedge 4
Reagent syringe
XEA019A Silicon grease 1
TIGHTENING TORQUE TABLE

TIGHTENING
REP SCREWS DESIGNATION TORQUES
(DIAG n°) mN.M (Ozf.in)
1 (1) 2x CHC M3x25 120 (17)
(6) 9x CHC M3x12 400 (56.8)
(6) 2x FX M3x12 400 (56.8)
NOTE
p. 2/10
RAS236B
SAMPLING PROBE
1 - O’ring replacement (1x FAA053A)

ü Switch on the instrument
ü Run Menu: Maintenance\Technician\Others\Maintenance carriage position
ü Open the pneumatic access door (Right side of the instrument)
ü Unscrew the 2 fixation screws (2x CHC M3x25) of the Probe rinsing block on the
6 MONTH MAINTENANCE
carriage (Diag.1)
Diag.1
Diag.2
ü Take out the Probe and the guide at the same time (lift the Probe locker to free the
Probe) (Diag.2 & 3)
ü Put the Probe back to its support
Diag.4
Diag.3
TORQUE
Rinsing block screws:
120mN.m (17 Ozf.in)
ü Open rinsing block assembly and replace the old O’ring joint by the new one in the 6
month maintenance kit (use one drop of silicon grease between 2 fingers for O’ring) (Diag.4)
ü Reassemble following previous steps backward
ü When startup is done check that there is no leak
p. 3/10
6 MONTH MAINTENANCE
REAGENTS SYRINGE
1 - O’ring replacement (1x FAA065A & 4x XDA622A)

ü Switch off the instrument and disconnect power supply cable
ü Open the left side panel and the mother board door (lock it)
WARNING
A flat cable is connected at the back of the mother board, becareful when you open the
door that you don’t disconnect or damage it
ü Disconnect the tubes (Diag.5):

Valve 6 inlet 3,
Valve 7 inlet 3,
Valve 8 inlet 3,
Valve 9 inlet 3,
Valve 11 inlet 3
ü Unscrew the 2 fixation screws of the reagents syringe (Diag.5)
Pentra 60
Diag.5
ü Retire gently the reagents syringe

ü Drain syringe pushing Pistons in a waste container
ü On a piece of absorbant paper open the reagents syringe removing the bottom plate
(9x CHC M3x12, 2x FX M3x12)
ü Replace O’ring joints by new one in the 6 month maintenance kit (use one drop of
silicon grease between 2 fingers for O’ring) (Diag.6)
p. 4/10
RAS236B
TORQUES
9x CHC M3x12:
400mN.m (56.8 Ozf.in)
2x FX M3x12:
6 MONTH MAINTENANCE
400mN.m (56.8 Ozf.in)
Diag.6
WARNING
A washer is adapted to each O’ring joint tore diameter, so that it is mandatory to keep
O’ring joint and its washer together.
Lyse piston O’ring had no washer.

ü When startup and prime are done check that there is no leak
p. 5/10
6 MONTH MAINTENANCE
DRAINING & COUNTING SYRINGES
1 - O’ring replacement on Draining Syringe (1x XDA621A)

ü Switch off the instrument
ü Disconnect power supply cable
ü Open the back side panel (6x CHC M4x6) (Diag.7)
Pentra 60
Diag.7
ü Disconnect the electronic connectors behind the instrument (Diag.8)
Diag.8 Diag.9
ü Cut the tyraps (Diag.9)

ü Disconnect grounding wire on the frame (Diag.9)
ü Put a piece of aborbant paper under the syringe and disconnect both tube
p. 6/10
RAS236B
6 MONTH MAINTENANCE
Diag.11
Diag.10
ü Unscrew the 4 CHC M4x16 syringe screws (just few turns to free silent block) (Diag.10)
ü Remove the syringe
ü Drain the syringe pushing the piston nut up and down several times
ü According to the type of syringe fitted, unscrew the 4x CHC M4x16 screws (Diag.11) or
unscrew the syringe body and open it (Diag.12).
Diag.12 Diag.13
ü Replace the O’ring (Diag.14) by the new one from the 6 month maintenance kit (use one drop
of silicon grease in between 2 fingers to lubricate O’ring and piston).
ü Reassemble in reverse order according to diagram 14 (Molded syringe body Diag.12 has to
be rotated until the mark on the body is in between the two marks of the motor Diag.15).
O’ring
Anti-extrusion ring
O’ring thickness
wedge
Diag.14
Diag.15
p. 7/10
6 MONTH MAINTENANCE
2 - O’ring replacement on Counting Syringe (1x XDA621A)

WARNING
ü Open the back side panel

Pentra 60
Diag.17
Diag.16

ü Put a piece of absorbant paper under the syringe and disconnect all the tubes from the
syringe (Diag.18)
Diag.19
Diag.18

ü Open the syringe (4x CHC M4x16 screws) (Diag.19)
p. 8/10
RAS236B
ü Replace the old O’ring joint and washer by new one from the 6 month maintenance kit (use
one drop of silicon grease in between 2 fingers to lubricate the O’ring) (Diag.22).
6 MONTH MAINTENANCE
Diag.20 Diag.21
ü Reassemble in reverse order according to diagram 22 (Molded syringe body Diag.20 has to
be rotated until the mark on the body is in between the two marks of the motor Diag.23).
O’ring
Anti-extrusion ring
O’ring thickness
wedge
Diag.22
Diag.23
p. 9/10
6 MONTH MAINTENANCE
INSTRUMENT CLEANING
ü Dilute the 12°cl bleach to 1 part of bleach for 4 of deionize water (1/5).
ü Instrument environment must be cleaned.
ü No sponge, nor cloth must be used. Only absorbant paper, thrown after use, in contamination
bins, have to be used. For small or sensitive assemblies use accurate drier papers.
ü All assemblies suspected to have been in contact with biohazardous material must be
disinfected with diluted bleach (the stainless steel must be bleached below 30°Celsius).
ü Blood stains or salt marks must be cleaned with spray detergent first.
ü Concerned assemblies:
• All the thermostated compartment

• Outer surfaces of the instrument (perpex, covers, reagent locations....)
• Waste connector plug
• Liquid valve push button
Pentra 60
• Assemblies close to the needle

• Tube holder assembly
• Overflow trays
ü Reinstall all the assemblies and setup the instrument back to its initial configuration.
ü Follow procedure RAS252 to check instrument accuracy and eventually re-calibrate it.
p. 10/10
YEARLY MAINTENANCE
• CONCERNS
• REQUIRED TOOLS
Hexagonal keys Philips screw driver
Dynamometric screw driver A302, A301, A300 Torx keys
Clamps
Scalpel
Cutting pliers
Flat screw driver
Pentra 60
Empty sample tubes
Silicone grease
Soft tissue
Liquid soap
Distilled water
Micropipette tip
RBC/PLT Latex
2h
• FREQUENCY
The yearly maintenance frequencies vary with the instrument output.
According to the number of cycle, 3 categories are created :
0 - 30 cycles/day -> 1 overall maintenances/year
30 - 120 cycles/day -> 2 overall maintenances/year
> 120 cycles/day -> 3 overall maintenances/year

Spare parts kit : XEA486BS
RAS237B
YEARLY MAINTENANCE
SAMPLING SYRINGE
5DIFF SYRINGE
OPTICAL BENCH LAMP
LMNE FLOWCELL COAXIAL
DILUENT RESERVOIR JOINT
DRAINING CHAMBER
COUNTING HEADS
WARNING
YEARLY MAINTENANCE KIT

Pentra 60
Part Number SPARE PARTS KIT XEA486BS Qty

DAJ007A Lamp 20W 9.5V 1
5 DIFF syringe
FAA046A O’ring 2.75x1.60 Viton 2
Coaxial cable
FAA064A O’ring 1.42x1.52 Fluorocarbon 2
Sampling syringe
Draining chamber
FAA067A O’ring 2.40x1.90 Fluorocarbon 4
5DIFF Syringe
GBG275A Counting head Aperture Joint 4
XEA663A LMNE flowcell coaxial kit 1
XEA286AS Waste chamber Joint + Washer 1
JAJ007A Polyeth. tape 1m50

TIGHTENING
REP (DIAG n°) SCREWS DESIGNATION TORQUES
mN.M (Ozf.in)
2 (4) 3x FX M3x12 400 (56.8)
4 (7) 2x FX M3x12 400 (56.8)
5 (7) 4x CHC M3x12 400 (56.8)
6 (18) 4x CHC M3x20 120 (17)
8 (24) 2x CHC M3x25 120 (17)
9 (26) 2x CHC M3x12 120 (17)
p. 2/12
RAS237B
SAMPLING SYRINGE

ü Disconnect the diluent tube between valve 18 and sampling syringe (Diag.1) The di-
luent is drained through the sampling syringe
ü Unscrew both fixation screws of the Sampling syringe on the carriage (Diag.2)
YEARLY MAINTENANCE
Diag.1 Diag.2
ü Disconnect from the syringe output tube (Diag.3)

ü Unscrew the 3 FX M3x12 screws from the bottom of the syringe (Diag.4)
Diag.3 Diag.4
ü Remove the bottom plate (3x FX M3x12), the piston

axle and the 2 O’ring joints TORQUE
ü Replace both O’ring joints by new one in the yearly
maintenance kit (use one drop of silicon grease
3x FX M3x12:
between 2 fingers for both O’ring) 400mN.m (56.8 Ozf.in)
ü When startup and prime are done check that there
is no leak
p. 3/12
YEARLY MAINTENANCE
5DIFF SYRINGE
1 - O’ring replacement (1x FAA040A & 4x FAA067A)

WARNING

Valve 2 inlet 2,
Valve 3 inlet 3,
Valve 5 inlet 1
ü Unscrew both fixation screws of the 5DIFF syringe (Diag.6)
ü Disconnect gently from the syringe the 2 left tubes
Pentra 60
Diag.5
Diag.6
ü Remove the bottom plate (2x FX M3x12, 4x CHC M3x12), the piston axles and the
O’ring joints (Diag.7)
Diag.7 Diag.8
p. 4/12
RAS237B
ü Replace all O’ring joints by new one in the yearly maintenance kit (use one drop of
silicon grease between 2 fingers for each O’ring and piston except the Diameter 12 piston
of the 5DIFF syringe) (Diag.8)
TORQUES
YEARLY MAINTENANCE
4x CHC M3x12: 400mN.m (56.8 Ozf.in)
2x FX M3x12: 400mN.m (56.8 Ozf.in)
p. 5/12
YEARLY MAINTENANCE
OPTICAL BENCH LAMP
1 - Lamp replacement (1x DAJ007A)

ü Open the cover
ü Disconnect the lamp supply (Diag.10)
ü Unscrew lamp fixation screws (few turns) (Diag.9)
Pentra 60
Diag.9
Diag.10
ü Turn the lamp and remove it (Diag.10)

ü Change the lamp
ü Put back the fixation system and block the screws
ü Reconnect the lamp supply
WARNING
Do not touch lamp bulbe with the fingers, use a piece of cloth or paper to manipulate
the lamp.
p. 6/12
RAS237B
LMNE FLOWCELL COAXIAL
1 - Coaxial replacement (1x XEA663A)

ü Open the cover
ü Open the left side panel and the mother board door (block it)
WARNING
YEARLY MAINTENANCE
ü Open the optical bench cover (4 screws Diag.11)
Diag.11
ü Disconnect the «T» connector (few diluent drops will leak) (Diag.12&13)
Diag.12
Diag.13
p. 7/12
YEARLY MAINTENANCE
ü Disonnect ground fitting and put the new LMNE coaxial instead
ü Reassemble following previous steps backward (except optical bench cover)
ü Run Menu: Maintenance\Hydraulics systems\Rinse\Cytometer to get rid of the air
bubbles stuck to the inner optical surfaces
ü Check that there is no leak and that flowcell contains no or just a very few air bubbles
ü Put back the optical bench cover
Diag.14
2 - LMNE Flowcell adjustment check

ü Run Menu: Maintenance\Technician\Measurement\LMNE Adjustment
Pentra 60
ü Mix RBC/PLT Latex thoroughly and press ESC to start sampling

ü When optical bench is well adjusted:
MEASUREMENT\LMNE ADJUSTMENT
LMNE LAMP
6.00
4.00 8.00
TRANSFERT 1TIME
- LEVEL/CGE
198
100 300
RESISTIVE CHANNEL
51
5 70
ABSORBANCE CHANNEL
180
10 240
Diag.15
ü Check that the displayed values are within target values range:
PARAMETER TARGET VALUE RANGE

LMNE LAMP 6.00 5.50 to 6.50
TRANSFER TIME 200 150 to 250
RESISTIVE CHANNEL 50 45 to 55
ABSORBANCE CHANNEL 180 180 ±10
ü If you are out of range perform a RAS240 LMNE Flowcell adjustment procedure
p. 8/12
RAS237B
DILUENT RESERVOIR JOINT
1 - Joint and Washer replacement (1x XEA286AS)

ü Run Menu: Maintenance\Hydraulics systems\Drain chamber\Diluent reservoir
ü When cycle is finished disconnect the drain tube under the reservoir
ü Unscrew the 2 fixation screws under diluent reservoir (few turns to release diluent
reservoir support) (Diag.16)
YEARLY MAINTENANCE
Diag.16
ü Lift reservoir, take care that no tube is diconnected from reservoir

ü Unscrew the 4 reservoir screws on top
ü Replace joint and washer by new one in the yearly maintenance kit
ü Locate new washer and joint against the reservoir top, then the diluent reservoir body
(Diag.17)
NOTE
Reservoir body must be turned to have
inferior support backward and tubes in the
upper part in frontward
TORQUE
4x CHC M3x20:
120mN.m (17 Ozf.in)
Diag.17
ü Screw the 4 reservoir screws on top

ü Put back diluent reservoir in its location and block screws
ü Connect drain tube under diluent reservoir
p. 9/12
YEARLY MAINTENANCE
DRAINING CHAMBER

ü Run Menu: Maintenance\Hydraulics systems\Drain chamber\All chambers
ü Turn gently the chamber’s bottom between 2 fingers (Diag.18)
Pentra 60
Diag.19
Diag.18
ü Replace the O’ring joint (Diag.19)
Diag.20
ü Repeat with the other chambers (Diag.20)

p. 10/12
RAS237B
COUNTING HEADS
1 - RBC/WBC Coaxial cable O’ring replacement (2x FAA046A)

ü Run Menu: Maintenance\Hydraulics systems\Drain chamber\All chambers
ü Put a piece of white paper or cloth under the Counting chamber assembly
ü Record the tube positions before dismantling the Counting chamber assembly
YEARLY MAINTENANCE
ü Disconnect the chamber tubes (not the wastes)
ü Dismantle the Counting chamber assembly, 3 screws (Diag.21)
Diag.21
ü Dismantle the electrode loosening the 2 fixation screws (Diag.22)
Diag.22 Diag.23
ü Use a previously cut micropipette tip to replace the electrode O’ring joint as shown in
diagram 25
ü Reassemble electrode
ü Repeat with the other electrode (Diag.23)
p. 11/12
YEARLY MAINTENANCE
2 - Counting head aperture joint replacement (4x GBG275A)

ü Carefully dismantle the counting head (Diag.24) and plunge the aperture in distilled
water
ü Replace both EPO joints by new one (Diag.25)
ü Clean the chamber and the counting head with liquid soap, do not introduce any sharp
instrument inside so as to avoid damaging the inside of the chamber and the aperture
Pentra 60
Diag.24
TORQUE
2x CHC M3x12:
120mN.m (17 Ozf.in) Diag.25
CAUTION
Do not manipulate the aperture using hard instruments. Clean the aperture with a
piece of soft paper or preferably, in between 2 fingers.
ü Rinse thoroughly with distilled water

ü Dry the exterior of the chamber with a soft paper
CAUTION
Do not apply too much pressure on the electrode fixation screws, as it can
break the aperture (tightening torque = 120mN.m / 17 Ozf.in).
It is recommended to reconnect the tubes on the counting head before
reassembling the "electrode/chamber/counting head" assy in order to avoid
applying constraint on the chamber.
ü Repeat operations for the other counting head

ü Reassemble electrode
ü Repeat with the other electrode (Diag.24)
p. 12/12
EVERY 2 YEARS MAINTENANCE
• CONCERNS
Note: This maintenance and 6 month maintenance has to be performed

at the same time
• REQUIRED TOOLS
Hexagonal keys Philips screw driver
Dynamometric screw driver A302, A301, A300 Torx keys
Clamps
Scalpel
Cutting pliers
Flat screw driver
Pentra 60
2 hours
• FREQUENCY
0 to 120 cycles per day: Every 2 years
>120 cycles per day: Every year

Spare parts kit: XEA581BS
RAS238B
SAMPLING PROBE
REAGENT SYRINGE
COUNTING/DRAINING SYRINGE, PISTON
WARNING
Pentra 60
EVERY 2 YEARS MAINTENANCE KIT
PISTONS KIT
Part Number Qty
XEA581BS
GBC031A Syringe, Lyse piston 1
GBC030A Syringe, Reagent piston 4
GBG260A Vacuum/Waste piston Spacer 2
GBG274A Syringe, Vacuum/Waste piston 2
GBG091A Carriage, Needle guide 1
XDA619AS Needle, Sampling needle 1
TIGHTENING
REP SCREWS
TORQUES
(DIAG n°) DESIGNATION
mN.m (Ozf.in)
1 (1) 2x CHC M3x25 100 (14.2)
(6) 9x CHC M3x12 700 (99.4)
(6) 2x FX M3x12 400 (56.8 )
p. 2/11
RAS238B
SAMPLING PROBE

1 - Probe and Probe Rinsing Block Replacement (1x XDA619AS & 1x GBG091A)
ü Unscrew both fixation screws of the Probe rinsing block on the carriage (Diag.1)
Diag.1 Diag.2
ü Take out the Probe and the rinsing block at the same time (lift the Probe locker to free
the Probe) (Diag.2&3)
ü Open guide assembly and replace the probe rinsing block and the probe (Diag.4)
Diag.4
Diag.3
IMPORTANT
Use a new FAA053A O’ring joint when performing 6 Month maintenance at the same
time

ü When startup is done check for that there is no leak TORQUE
Rinsing block screws:
100mN.m (14.2 Ozf.in)
p. 3/11
REAGENTS SYRINGE
1 - Pistons replacement (1x GBC031A & 4x GBC030A)

ü Switch off the instrument and disconnect power supply cable
ü Open the left side panel and the mother board door (block it)
WARNING

Valve 6 inlet 3,
Valve 7 inlet 3,
Valve 8 inlet 3,
Valve 9 inlet 3,
Valve 11 inlet 3
Pentra 60
ü Unscrew both fixation screws of the reagents syringe (Diag.5)
Diag.5
ü Retire gently the reagents syringe
WARNING
A washer is adapted to each O’ring joint tore diameter, so that it is mandatory to keep
O’ring joint and its washer together
p. 4/11
RAS238B
ü Drain syringe pushing Pistons in the waste container

ü On a piece of absorbant paper open the reagents syringe removing the bottom plate

(9x CHC M3x12, 2 FX M3x12)
Diag.6
ü Replace Pistons by new ones in the every 2 years maintenance kit (use one drop of
silicon grease between 2 fingers for each piston) (Diag.6)
IMPORTANT
Be sure to use the new washers corresponding to the new O’ring joints, when
performing 6 Month maintenance at the same time
TORQUES
9x CHC M3x12: 400mN.m (99.4 Ozf.in)
2x FX M3x12: 400mN.m (56.8 Ozf.in)

ü When startup and prime are done check for that there is no leak
p. 5/11
COUNTING/DRAINING SYRINGE
1 - Piston replacement on Vacuum pump (1x GBG274A + 1x GBG260A)

ü Open the back side panel (Diag.7)
Pentra 60
Diag.7
Diag.8 Diag.9
ü Cut the tyraps (Diag.9)

ü Put a piece of aborbant paper under the syringe and disconnect both tube
p. 6/11
RAS238B

Diag.11
Diag.10
ü According to the type of syringe fitted, remove the syringe guiding plate (4 torx screws)
(Diag.11)
Diag.12 Diag.13
p. 7/11
ü Remove the 3 piston’s screws (CHC M3x16) (Diag.14)
Diag.14
Pentra 60
2
IMPORTANT
Take care to keep syringe nut in place on the motor screw
1
ü Piston replacement:
Old model of syringe: Use parts 1 + 2
New model of syringe: Use part 1
ü Replace Piston by new one in the every 2 years maintenance kit (use a bit of silicon
grease)
WARNING
Be sure to use the new washer corresponding to the new O’ring joint, when performing
6 Months maintenance at the same time
ü Reassemble in reverse order according to diagram 15 (Molded syringe body has to be rotated
until the mark on the body is in between the two marks of the motor Diag.16).
O’ring
Anti-extrusion ring
O’ring thickness
wedge
Diag.15
Diag.16
p. 8/11
RAS238B
2 - Piston replacement on Waste pump (1x GBG274A + 1x GBG260A)


WARNING
ü Open the back side panel (Diag.17)
Diag.17

ü Unscrew the 4 syringe screws (just few turns to free silent block) (Diag.19)
Diag.19
Diag.18
ü Disconnect grounding wire from the frame (Diag.19)
p. 9/11
ü Put a piece of aborbant paper under the syringe and disconnect all the tubes from the
syringe (Diag.20)
Pentra 60
Diag.20 Diag.21

ü According to the type of syringe fitted, remove the syringe guiding plate (4 torx screws)
(Diag.21)
Diag.23
Diag.22
p. 10/11
RAS238B

Diag.24
ü Remove the 3 piston’s screws (CHC M3x16) (Diag.24)

2
IMPORTANT
Take care to keep syringe nut in place on the motor screw 1
ü Piston replacement:
Old model of syringe: Use parts 1 + 2
New model of syringe: Use part 1
ü Replace Piston by new one in the every 2 years maintenance kit (use a bit of silicon
grease)
WARNING
Be sure to use the new washer corresponding to the new O’ring joint, when performing
6 Month maintenance at the same time
ü Reassemble in reverse order according to diagram 25 (Molded syringe body has to be rotated
until the mark on the body is in between the two marks of the motor Diag.26).
O’ring
Anti-extrusion ring
O’ring thickness
wedge
Diag.25
Diag.26
p. 11/11
DECONTAMINATION & RINSE
• CONCERNS
Instrument decontamination before maintenance operation in the following
cases:
- Instrument removed from a biohazardous area
- Maintenance intervention on contaminated assemblies
and instrument rinse
• REQUIRED TOOLS
Hexagonal keys
Clamps
Flat screw driver
Torx keys
Pentra 60
Fungicidal, bactericidal, virus killing detergent spray, non corrosive for metals,
Non plastic altering.
Bleach solution 12°Cl
Deionize water
Protection gloves
Absorbant paper
Distilled water
1h35min
• FREQUENCY
On request
RAS239B
DECONTAMINATION
1 - Preparation (20min)
ü Run Menu: Maintenance\Hydraulics systems\Concentrated cleaning (Diag.1)
WARNING
Disposable gloves and lab coat must be worn by the operator
Local or national regulations must be applied in at all the times
MAITENANCE\HYDRAULICS SYSTEMS 16:05 MAINTENANCE\HYDRAULICS SYSTEMS 16:05

Pentra 60
1-BACKFLUSH
1- BACKFLUSH
2-RINSE
2- RINSE 3-DRAIN CHAMBERS
3- DRAIN CHAMBERS 4-CONCENTRATED CLEANING
4- CONCENTRATED CLEANING
CYCLE IN PROGRESS. PLEASE WAIT......

21%
Diag.1 Diag.2
MAINTENANCE\HYDRAULICS SYSTEMS 16:05
POUR2-HYDRAULICS
3 ml OF MINOCLAIR INTO CHAMBERS
SYSTEMS
PRESS ESC KEY TO CONTINUE
3-MECHANICAL SYSTEMS
CYCLE IN PROGRESS. PLEASE WAIT......

31%
Diag.3
ü Pour 3ml of Minoclair in each chamber

ü Press ESC to continue
ü When cycle is finished, switch off the instrument and remove the supplying cable
ü Open the instrument cover
ü Spray the bactericidal cleaner on all biohazardous areas and wait for 10 minutes
(assemblies in contact with the biohazardous material such as instrument cover, tube
holder, keyboard, start key, assemblies close to the SN...
p. 2/4
RAS239B
2 - Manual decontamination (20 min)

ü Dilute the 12°cl bleach to 1 part of bleach for 4 of deionize water (1/5)
ü Instrument environment must be cleaned and decontaminated
ü No sponge, nor cloth must be used. Only absorbant paper, thrown after use, in conta-
mination bins, have to be used. For small or sensitive assemblies use accurate drier

papers
ü All assemblies suspected to have been in contact with biohazardous material must be
disinfected with diluted bleach (the stainless steel must be bleached below 30°Celsius)
ü Blood stains or salt marks must be cleaned with spray detergent first
• Concerned assemblies
ü Outer surfaces of the instrument (perpex, covers, LCD, reagent locations....)
ü Keyboards
ü Waste connector plug
ü Liquid valve push button
ü Assemblies close to the needle
ü Tube holder assembly
ü Overflow trays
ü Reinstall all the assemblies and setup the instrument back to its initial configuration
3 - Analysis circuit decontamination (30 min)

ü Prepair 1 bottle containing 1/2 litre of bleach diluted to 1 part of bleach for 9 parts of
deionize water (1/10)
ü Prepair 1 bottle containing 1/2 litre of distilled water
ü Replace the reagent bottles by the diluted bleach bottle
ü Run 6 All reagents prime cycles Menu: Reagents\Prime\All Reagents (Diag.4)
REAGENTS\PRIME 16:05 REAGENTS\LEVEL/CHANGE 16:05
DILUENT
1- DILUENT 100% CHANGE
2- CLEANER EOSINOFIX 1 - LEVEL/CHANGE
3- EOSINOFIX 99% CHANGE
4- BASOLYSE II BASOLYSE II
5- ALPHALYSE 98% CHANGE
6- ALL REAGENTS CLEANER
7- UNPRIME ALL 98% CHANGE
ALPHALYSE
98% CHANGE
Diag.4 Diag.5
ü Fill a sample tube with diluted bleach to 1 part of bleach for 4 of deionize water (1/5)
ü Run 15 cycles Burn-in Menu: Technician\User mode\Burn-in leave the instrument
operating until it stops
p. 3/4
DECONTAMINATION
1 - Drain and rinse (30 min)

ü Remove the 3 reagent straws from the bottle containing the diluted bleach
ü Wrap the straws in absorbant paper
ü Run 2 All reagents prime cycles Menu: Reagents\Prime\All Reagents (Diag.4) the
bleach is drained
ü Replace the diluted bleach by the distilled water bottle and re-plunge the straws in
distilled water
ü Run 6 All reagents prime cycles Menu: Reagents\Prime\All Reagents (Diag.4) (Rinse)
ü Remove the 3 reagent straws from the distilled water (Wrap the straws in absorbant
paper)
ü Run 2 All reagents prime cycles Menu: Reagents\Prime\All Reagents (Diag.4) the
distilled water is drained
ü Run a STAND BY cycle
ü Re-install the reagent bottles and the straws
ü Close the instrument cover
Pentra 60
WARNING
When rinse is finished check that LMNE chamber is empty
p. 4/4
LMNE FLOWCELL ADJUSTMENT
• CONCERNS
Instrument adjustment:
LMNE Flowcell
• REQUIRED TOOLS
Hexagonal keys
Flat screw driver
Torx keys
Pentra 60
Latex RBC\PLT
Fresh blood samples
Calibrator
1h
• FREQUENCY
On request

None
RAS240B
LMNE FLOWCELL CHECK & ADJUSTMENT
WARNING
Pentra 60
p. 2/6
RAS240B
LMNE FLOWCELL CHECK & ADJUSTMENT

ü This adjustment procedure must be carried out when LMNE flowcell is moved or replaced,
or when LMNE tube is changed. No adjustment is required when LMNE Lamp is replaced
ü Menu: Maintenance\Technician\Measurement\LMNE Adjustment allows the
adjustment of the entire optical bench parameters. A 1:400th dilution of the RBC/PLT
Latex is prepared (5µl of Latex, 2ml of diluent) in the LMNE chamber. This dilution is then
transfered toward the optical chamber and injected
ü The measurments are continuously displayed every 700ms during a total of 27 seconds
WARNING
Never dismantle Emission gun or even unlock it. The adjustment is factory made and
cannot be performed in the field.
1 - Preparation
ü Open the cover
ü Open the left side panel and the main board door (block it)
WARNING
Diag.1
ü Run Menu: Maintenance\Hydraulics systems\Rinse\Cytometer to get rid of air bubbles

stuck to the inner optical surfaces
ü Check that the flowcell contains no or just a very few air bubbles
p. 3/6
2 - LMNE Flowcell adjustment check

IMPORTANT
Mix RBC/PLT LATEX thoroughly and for few minutes !
ü Press ESC to start sampling

ü The following screen is displayed (When optical bench is well adjusted):
MEASUREMENT\LMNE ADJUSTMENT
LMNE LAMP
6.00
4.00 8.00
TRANSFERT 1TIME
- LEVEL/CGE
Pentra 60
198
100 300
RESISTIVE CHANNEL
51
5 70
ABSORBANCE CHANNEL
180
10 240
Diag.2
ü Check that the displayed values are within the range:
PARAMETER TARGET RANGE

VALUE
LMNE LAMP 6.00 5.50 to

6.50
TRANSFER TIME 200 150 to 250
RESISTIVE CHANNEL 50 45 to 55
Max has to be
ABSORBANCE CHANNEL >170 reached
Diag.3
ü If it is out of range or to get a better adjustment follow this procedure
p. 4/6
RAS240B
3 - LMNE Flowcell position adjustment


IMPORTANT
Mix LATEX thoroughly and for a few minutes !
ü Press ESC to start sampling

ü Adjust LMNE lamp voltage around 6V:
- Adjust by R11 potentiometer located on optical bench board (Diag.4)
NOTE Test points on Optical bench board, near R11 potentiometer, can be
used to check 6V with a voltmeter.
R11
Diag.4
ü Adjust Resistive channel to 50:

- Adjustment is made by R136 potentiometer located on mother board (Diag.7
on next page)
ü Adjust Absorbance channel to the maximum (and above 170):
- Adjustment is made by lateral or axial reception gun movement, use toothed
wheel (Diag.5) and lateral screw (Diag.6)
Diag.5 Diag.6
If you have difficulties to adjust Absorbance, you can increase gain

NOTE with the R148 potentiometer (Diag.7 on next page). Once you get the
highest absorbance adjustment, decrease slowly gain (R148) to get a
correct optical gain adjustment with normal fresh blood.
p. 5/6
ü Check that Transfer time is in limits of range (around 200):

- Transfer time is not adjustable and must be between 150 and 250
Transfer time bargraph is used to ensure the correct Right/Left loca-

NOTE tion of the light rectangle in the flowcell. When the optimal value is
reached the transfer time is around 200 because of the constant height
between guns alignment and flowcell.
This height is guaranteed by the washers under the flowcell, always
put them back even when the flowcell is replaced by a new one.
Pentra 60
R148
R136
Diag.7
ü Put back the Optical Bench cover
After intervention check, with normal fresh blood, that you have a cor-
NOTE rect matrix.
p. 6/6
CHAMBERS ADJUSTMENT
• CONCERNS
Chambers assembly adjustment
• REQUIRED TOOLS
Hexagonal keys
Flat screw driver
Pentra 60
15min
• FREQUENCY

None
RAS241B
CHAMBERS ADJUSTMENT
CHAMBERS ADJUSTMENT
WARNING
Pentra 60
p. 2/5
RAS241B
CHAMBERS ADJUSTMENT
ü This adjustment procedure must be carried out when chamber assembly is moved or
replaced.
1 - Alignment Check
ü Open the cover and the right access door
CHAMBERS ADJUSTMENT
ü Menu: Maintenance\Technician\Others\Maintenance carriage position
ü Locate probe as shown in diagrams 1 & 2
Diag.1 Diag.2
ü Push the carriage backward (without lifting the probe), check that there is the same
height between probe and first and last tops of chamber (Diag.3 & 4)
Diag.3 Diag.4
ü If you need to get a better adjustment follow this procedure
p. 3/5
CHAMBERS ADJUSTMENT
2 - Alignement Adjustment
ü Free the chamber support by loosening the 4 screws (Diag.5)
Diag.5
Pentra 60
ü Locate probe as shown in diagrams 6 & 7
Diag.6
Diag.7
ü Tighten gently the left upper screw
p. 4/5
RAS241B
ü Push the carriage backward (without lifting the probe), check that there is the same
height between probe and last top of chamber (Diag.8 & 9)
CHAMBERS ADJUSTMENT
Diag.8 Diag.9
ü If necessary adjust position moving up and down chamber
ü Perform an alignment check as described at the begenning of this procedure
ü When adjustment is correct, tighten screws and perform a Probe Adjustment procedure
WARNING
It is mandatory to perform the Probe adjustment procedure after this adjustment
p. 5/5
PROBE ADJUSTMENT
• CONCERNS
Probe adjustment
• REQUIRED TOOLS
None
Pentra 60
None
15 min
• FREQUENCY
On request

None
RAS242B
PROBE ADJUSTMENT
INTRODUCTION
NEEDLE HOME CHECK & ADJUSTMENT
ALL CARRIAGE POSITIONS CHECK & ADJUSTMENT
WARNING
Pentra 60
p. 2/7
RAS242B
INTRODUCTION
IMPORTANT
Check that the chamber assy is parallel to the carriage motion before checking or
making an adjustment of the Probe position. Follow CHAMBERS ADJUSTMENT
procedure of this manual
PROBE ADJUSTMENT
ü Probe adjustments can be accessed by the menu «Maintenance\
Technician\Dilution» as explained below (Diag.1):
ü Needle Home: Adjustment to get ü Check (2): Allows to ü Check (1): Allows to
the best probe extremity cleaning check that probe is aligned check probe cleaning
with LMNE-3 inlet position
ü Carriage Home: Allows

adjustment of the carriage sampling MAITENANCE\TECHNICIAN\DILUTION 16:05
position
NEEDLE HOME 29 CHECK
CARRIAGE HOME 17
NEEDLE POS 383 CHECK
ü Needle position: Probe Up/Down DIL1 CARRIAGE POS. 1127
adjustment
GB/BASO CARRIAGE POS. 1062
LMNE CARRIAGE POS 737
RBC CARRIAGE POS. 1054
CHECK CYCLE
ü All Carriage pos.: Probe Left/
Right adjustment
Diag.1
ü Check cycle: Allows to check that probe is aligned

with each distribution inlets
IMPORTANT
Running Check functions check the Probe positions before any adjustment.
p. 3/7
PROBE ADJUSTMENT
NEEDLE HOME CHECK & ADJUSTMENT
IMPORTANT
When the probe is in «NEEDLE HOME» position it is also in cleaning position.
Check and adjust the NEEDLE HOME position to achieve the best cleaning of the
probe extrimity
1 - Needle Home Check
ü Open the right access door

ü Run Maintenance\Technician\Dilution\Check (1) (Diag.1)
ü Check that the probe is about 9.4mm out from the carriage, compare with a 9.4mm
piece of paper that you have previously prepared (Diag.2&3)
Pentra 60
Diag.2 Diag.3
ü Press ESC to continue
2 - Needle Home Adjustment

ü Run Maintenance\Technician\Dilution\Check (1) (Diag.1)
ü Check that the probe is about 9.4mm out from the carriage, compare with a 9.4mm
piece of paper that you have previously prepared (Diag.2&3)
ü If Probe is too low (gap>9.4mm) increase Needle Home number of step
ü If Probe is too high (gap<9.4mm) decrease Needle Home number of step
ü If necessary adjust Needle Home value and re-run a Check (1) cycle to control the
gap again
p. 4/7
RAS242B
ALL CARRIAGE POSITIONS CHECK & ADJUSTMENT
IMPORTANT
To check the right position of the probe you must see inlet as in Diagram 4 and not
as in diagram 5 (where circles are not concentric)
PROBE ADJUSTMENT
Diag.4 Diag.5
1 - All Carriage Positions Check

ü Run Maintenance\Technician\Dilution\Check cycle (Diag.1)
ü The sampling needle moves over a chamber
ü Check in front of the inlet the right location of the probe: Disconnect the tube connected
to the inlet, if the probe is well adjusted you will see it as in the following diagram (Diag.6):
Diag.6
ü Press ESC key to move to the next chamber, and continue until the end of the cycle
ü When the cycle is ended check that no tube is disconnected
2 - All Carriage Positions Adjustment
IMPORTANT
Probe Up/Down adjustment is the same for all the chambers and cannot be modified
from one chamber to another (adjustment by the «NEEDLE POSITION» value)
Probe Left/Right position in the chambers can be adjusted separately for each
chamber (except the Rinse chamber)

ü Before any adjustment check the probe position in each chamber with the
Maintenance\Technician\Dilution\Check cycle option (Diag.1)
p. 5/7
PROBE ADJUSTMENT
Left/Right adjustment:
ü Adjust probe position for all the

chambers separately in the software MAITENANCE\TECHNICIAN\DILUTION 16:05
cycle order (Diag.7&8)
NEEDLE HOME 29 CHECK
CARRIAGE HOME 17
NEEDLE POS 383 CHECK
DIL1 CARRIAGE POS. 1127
GB/BASO CARRIAGE POS. 1062

LMNE CARRIAGE POS 737
RBC CARRIAGE POS. 1054
CHECK CYCLE
Diag.7
ü If you want to modify only one of the position, remind to increase or decrease the next
motion with the same number of steps, not to loose the other adjustments.
Pentra 60
S.
E PO
IAG S.
RR
CA
E PO
O
BA
S IAG
B/ A RR
G
(2) EC
) L MN
(3
.
S. P OS
PO GE
IAG
E
RIA
2
ARR C AR
C
1 BC
DIL (4)
R
(1) 4
3
1
Diag.8
p. 6/7
RAS242B
ü The right location for the probe is when you see it through the inlet as in the following
diagram (Diag.9):
Diag.9
PROBE ADJUSTMENT
ü When the cycle is ended check that no tube is diconnected
Up/Down adjustment:
ü If the Probe is too high increase Needle position value:
Diag.10
ü If the Probe is too low decrease Needle position value:
Diag.11
p. 7/7
MAIN BOARD ADJUSTMENT
• CONCERNS
Main board adjustment
• REQUIRED TOOLS
Small flat screw driver
Hexagonal keys
Voltmeter
Pentra 60
None
30min
• FREQUENCY
On request

None
RAS243B
HB BLANK ADJUSTMENT
APERTURE CURRENT CHECK
RBC/PLT GAIN ADJUSTMENT
WBC/BASO GAIN ADJUSTMENT
DRAIN SENSOR ADJUSTMENT
LMNE SENSOR ADJUSTMENT
MOTOR CURRENT ADJUSTMENT
THRESHOLDS ADJUSTMENT
WARNING
Pentra 60
p. 2/10
RAS243B
HGB BLANK ADJUSTMENT
1 - HGB Blank Adjustment

ü Open the cover and the left access door to main board
ü Run Menu: Maintenance\Technician\Measurment\HGB Blank Adjustment (Diag.1)

ü The utility carries out a rinsing cycle of the WBC chamber and continuously
displays the voltage used by the converter. The adjustment cycle lasts for 20 seconds.
ü The adjustment is carried out with the R248 potentiometer (Diag.2)
ü The final adjustment voltage is approximately 4.7 Volts +/- 0.05V
ü Once adjustment is done, check correct voltage between TP1 and ground.
MEASUREMENT\HGB BLANK ADJUSTMENT 6:45
HGB BLANK ADJUSTMENT

4.77 VOLTS
4.00 5.00
Diag.1
NOTE This adjustment is made with the right side door closed and under
stabilised thermic conditions (5min at least after startup)
Diag.2
p. 3/10
APERTURE CURRENT CHECK
1 - Aperture Current Check

ü Disconnect LMNE CIS, RBC and WBC coaxes (Diag.3)
Diag.3
Pentra 60
ü Run Menu: Maintenance\Technician\Measurement\Aperture Current (Diag.4)
MAINTENANCE\TECHNICIAN\MEASUREMENT 6:43
CHECK APERTURE CURRENT

PRESS ESCAPE TO CONTINUE
5-LMNE ADJUSTMENT
Diag.4
NOTE Aperture current can not be adjusted
WARNING
Do not damage connectors while measuring
IMPORTANT
Use an old coaxe that you have previously cut to control voltages with the voltmeter
ü Check Aperture currents in J22, J23 and J24, aperture current measured must be
around 60V (not ajustable)
ü Press ESC to exit
ü Reconnect LMNE CIS, RBC and WBC coaxes (Diag.3)
p. 4/10
RAS243B
RBC/PLT GAIN ADJUSTMENT
1 - RBC/PLT Gain Adjustment

ü A special cycle allows the dilution of the RBC/PLT LATEX to a 1/220th (10 µl of LATEX

with 2.2 ml of diluent). A special count programme carries out the calculations of the
mean volume in the predefined zones and displays them every 700 ms. The duration of
the measurement cycle is 21 seconds
ü The adjustments are made using R133 potentiometer for the RBC and R135 for the PLT
(Diag.7)
ü Run Menu: Maintenance\Technician\Measurement\RBC/PLT Gain (Diag.5)
ü Two graphs allow a visual check of the adjustments (Diag.6)
MEASUREMENT\RBC/PLT GAIN 6:43 MEASUREMENT\RBC/PLT GAIN 6:45
RBC
77
PRESS ESCAPE TO SAMPLE THE LATEX 56 96
PLT
111
80 135
Diag.5 Diag.6
RBC TARGET VALUE 78

PLT TARGET VALUE 112
IMPORTANT
Mix RBC/PLT Latex thoroughly before sampling
Diag.7
p. 5/10
WBC/BASO GAIN ADJUSTMENT
1 - WBC/BASO Gain Adjustment

ü A special cycle allows the dilution of the RBC/PLT LATEX to a 1/733rd (3µL of LATEX
with 2.2 ml of diluent). A special count programe carries out the calculations of the mean
volume in the predefined zones and displays them every 700 ms. The duration of the
measurement cycle is 21 seconds
ü The adjustments are made with the aid of a R134 potentiometer (Diag.10)
ü Run Menu: Maintenance\Technician\Measurement\WBC/BASO Gain (Diag.8)
ü A graph allows a visual assessment of the adjustments (Diag.9)
MEASUREMENT\WBC/BASO GAIN 6:43 MEASUREMENT\WBC/BASO GAIN 6:45
WBC/BASO
PRESS ESCAPE TO SAMPLE THE LATEX 101
57 156
Diag.8 Diag.9
Pentra 60
GB/BASO TARGET VALUE 102
IMPORTANT
Mix Latex thoroughly before sampling
Diag.10
p. 6/10
RAS243B
DRAIN SENSOR ADJUSTMENT
1 - Drain Sensor Adjustment

ü Run Menu: Maintenance\Technician\Sensor Check\Draining (Diag.11)

ü The utility empties the drain cell in order to adjust the voltage to 4.5Volts +/- 0.3 (test
point TP52, R287 potentiometer on the mother card Diag.13)
ü The utility then fills the sensor with the diluent. check that the voltage on TP52 is less
than 1 Volt (Diag.12)
TECHNICIAN\SENSOR CHECK 11:43 TECHNICIAN\SENSOR CHECK 11:43
ADJUST SENSOR VOLTAGE

PRESS ESCAPE TO CONTINUE CHECK SENSOR VOLTAGE
Diag.11 Diag.12
Diag.13
p. 7/10
LMNE SENSOR ADJUSTMENT
1 - LMNE Sensor Adjustment

ü Run Menu: Maintenance\Technician\Sensor Check\LMNE Transfer (Diag.14)
ü The utility empties the drain cell in order to adjust the voltage to 4.5Volts +/- 0.3 (test
point TP48, R286 potentiometer on the mother card Diag.16)
ü The utility then fills the sensor with the diluent. check that the voltage on TP48
is less than 1 Volt (Diag.15)
TECHNICIAN\SENSOR CHECK 11:43 TECHNICIAN\SENSOR CHECK 11:43
ADJUST SENSOR VOLTAGE

PRESS ESCAPE TO CONTINUE CHECK SENSOR VOLTAGE
Diag.14 Diag.15
Pentra 60
Diag.16
p. 8/10
RAS243B
MOTOR CURRENT ADJUSTMENT
1 - Motor Current Adjustment

ü Check and adjust, when necessary, all following voltages:

TEST
MOTORS POINTS VOLTAGE POTENTIO.
Draining syringe TP5 4.8V + 0.05 V R149

Counting syringe TP6 4.8V + 0.05 V R150
Dilutor syringe TP7 4V + 0.05 V R151
Optical Bench TP8 3V + 0.05 V R152
Injector syringe
Horizontal carriage TP10 3V + 0.05 V R154
Sample syringe TP11 2V + 0.05 V R155
Probe carriage TP12 4.5V + 0.05 V R156
ü Potentiometers and Test points are located on Main board as shown on the next diagram:
Draining syringe
Counting syringe
Reagents syringe
Optical Bench
Injector syringe
Horizontal carriage
Sample syringe
Probe carriage
Up/Down
Diag.17
p. 9/10
THRESHOLDS ADJUSTMENT
1 - Thresholds Adjustment
ü Check and adjust, when necessary, all following voltages:
THRESHOLDS TEST POINTS VOLTAGE POTENTIOMETERS

BASO TP14 300 mV + 5 R157
RBC TP13 300 mV + 5 R158
PLT TP2 300 mV + 5 R159
LMNE CIS TP3 650 mV + 5 R160
LMNE OD TP4 350 mV + 5 R161
ü Potentiometers and Test points are located on Main board as shown on the next diagram:
Pentra 60
Diag.18
PLT
LMNE OD LMNE CIS RBC BASO
p. 10/10
THERMIC ADJUSTMENT
• CONCERNS
Concerns the enclosed thermic and the preheating of the reagents
• REQUIRED TOOLS
Thermometer
Pentra 60
None
30min
• FREQUENCY
On request

None
RAS244B
THERMIC ADJUSTMENT
THERMIC CHECKS
WARNING
Pentra 60
p. 2/7
RAS244B
THERMIC CHECKS
1 - Reagent Heating System Temperature Check

ü Concerns the checking of the reagent heating system
ü Open both instrument side to open the cover
ü Enter Menu: Maintenance\Technician\Heating Systems\Reagent heating system\
(Diag.1)
THERMIC ADJUSTMENT
TECHNICIAN\HEATING SYSTEMS 15:38
1-REAGENT HEATING SYSTEM

2-THERMOSTATED COMPARTMENT
Diag.1
ü Adjustment: Allows the cap- REAGENT HEATING SYSTEM 15:38
ture of the CTN value

1-ADJUSTMENT
2-REFERENCE
ü Reference: Allows the modifi-

cation of the temperature instruc- Diag.2
tions
IMPORTANT
This control must be made with the right side door closed and under stabilised
thermic conditions (5min at least after startup)
Diag.3
ü Close the right side door

ü Put the thermometer probe in the LMNE chamber (the one in the middle) (Diag.3)
IMPORTANT
Make sure to have plunged the thermometer probe in the liquid
p. 3/7
THERMIC ADJUSTMENT
ü Select number of cycle to run: 5 (Diag.4)

ü Run Menu: Maintenance\Technician\Heating Systems\Reagent heating
system\Check (Diag.4)
REFERENCE 15:08
REFERENCE TEMPERATURE 35.0
RUN CYCLE NUMBER 5 CHECK
RUNNING TEMPERATURE LIMITS

MIN 33.0 MAX 37.0
Diag.4
ü Every time you hear one beep read the Thermometer value: it must be 35°C +/-1°C
To validate check you must get, during control, 10 times the right
NOTE temperature
If an adjustment is required, when heating block is replaced, follow this
procedure
Pentra 60
p. 4/7
RAS244B
2 - Reagent Heating System Temperature Adjustment

ü Enter Menu: Maintenance\Technician\Heating Systems\Reagent heating
system\Adjustment (Diag.5)
ADJUSTMENT 15:05
CTN VALUE 664
THERMIC ADJUSTMENT
Diag.5
ü Check and eventualy change the CTN value
The reagent heating system CTN value is located on the CTN wire near
NOTE the main board (left connector on J31) (Diag.6)
Be sure you read the right value, sticker could be up or down
ü A LED near the connector shows

the heating command:
- when lit off, heating is off
- when blinking, heating is on
- when lit on, heating is maximum
Diag.6
ü Make a reagents preheating temperature check (at the begining of this procedure)
IMPORTANT
ü If you don’t get the right temperature (35°C +/-1°C), adjust it by the mean of the
Reference temperature (Diag.7)
REFERENCE 15:08

MIN 33.0 MAX 37.0
Diag.7
ü When Reference temperature increases, reagent preheating temperature increases

ü Make a reagents preheating temperature check (at the begining of this procedure)
ü If required restart adjustment and check again, until you get an acceptable
temperature
p. 5/7
THERMIC ADJUSTMENT
3 - Thermostated Compartment Temperature Check

ü Open both instrument side to open the cover
ü Enter Menu: Maintenance\Technician\Heating Systems\Thermostated
Compartment\ (Diag.8)
TECHNICIAN\HEATING SYSTEMS 15:38 THERMOSTATED COMPARTMENT 15:38
1-REAGENT HEATING SYSTEM 1-ADJUSTMENT

2-THERMOSTATED COMPARTMENT 2-REFERENCE
Diag.8 Diag.9
ü Adjustment: Allows the cap- ü Reference: Allows the modifi-

ture of the CTN value cation of the temperature instruc-
tions
IMPORTANT
Pentra 60
Diag.10
ü Put the thermometer probe in the CTN sensor (Diag.10)

ü Select number of cycle to run: 5 (Diag.11)
ü Run Menu: Maintenance\Technician\Heating Systems\Thermostated
Compartment\Check (Diag.11)
REFERENCE 15:08

MIN 33.0 MAX 37.0
Diag.11
ü Every time you hear one beep read the Thermometer value: it must be 35°C +/-1°C
To validate check you must get, during control, 10 times the right
NOTE temperature
If an adjustment is required, when heating block is replaced, follow this
procedure
p. 6/7
RAS244B
4 - Thermostated Compartment Temperature Adjustment

ü Enter Menu: Maintenance\Technician\Heating Systems\Thermostated
Compartment\Adjustment (Diag.12)
ADJUSTMENT 15:05
CTN VALUE 664
THERMIC ADJUSTMENT
Diag.12
ü Check and eventualy change the CTN value, thermostated compartment CTN value
is located on the CTN wire near the main board (right one connected on J33) (Diag.13)
The thermostated compartment CTN value is located on the CTN wire

NOTE near the main board (right connector on J33) (Diag.13)
Be sure you read the right value, sticker could be up or down
ü A LED near the connector shows

the heating command:
- when lit off, heating is off
- when blinking, heating is on
- when lit on, heating is maximum
Diag.13
ü Make a thermostated compartment temperature check (previous page of this

procedure)
IMPORTANT
ü If you don’t get the right temperature (35°C +/-1°C), adjust it by the mean of the
Reference temperature (Diag.14)
REFERENCE 15:08

MIN 33.0 MAX 37.0
Diag.14
ü When Reference temperature increases, reagent preheating temperature increases

ü Make a thermostated compartment temperature check (previous page of this
procedure)
ü If required restart adjustment and check again, until you get an acceptable
temperature
p. 7/7
VACUUM ADJUSTMENT
• CONCERNS
Concerns counting and draining syringe vacuum control and adjustment
• REQUIRED TOOLS
Barflex
Pentra 60
None
15min
• FREQUENCY
On request

None
RAS245B
VACUUM ADJSUTMENT
DRAINING SYRINGE VACUUM CHECK

COUNTING SYRINGE VACUUM CHECK & ADJUSTMENT
WARNING
NOTE No adjustment available for draining syringe
ü When installed in altitude, some intrument can exhibit some «Transfer Sensor Timeout»
alarms. This is due to the decreased atmospheric pressure. In these conditions, the draining
vacuum can lower down to about 200mb/210mb instead of 260mb (20% less): this results in
Pentra 60
a too long transfer time of the solution between LMNE chamber to T1-T2 segment before the
flowcell.
ü Hydraulic tubing modifications:

In such cases, we recommend to replace some 1.02 tubing (P/n: EAE005AS) by 1.3 tubing (P/n:
EAE006AS) in the following locations:
<1> from valve #4 / port 2 to «T2»: 1.02x130 replaced by 1.3x130.
<2> from «T2» to bottom right of 5Diff syringe: 1.02x240 replaced by 1.3x240.
<3> from top right of 5Diff syringe to valve #5 / port 1: 1.02x95 replaced by 1.3x95.
IMPORTANT
The tubing length must remain identical
1
2
p. 2/5
RAS245B
DRAINING SYRINGE VACUUM CHECK
1 - Draining Syringe Vacuum Check

ü Disconnect the tube on the draining syringe and replace it by the Barflex tube (Diag.1)
VACUUM ADJUSTMENT
Diag.1
ü Enter Menu: Maintenance\Technician\Vacuum Check\Draining\ (Diag.2&3)
TECHNICIAN\VACUUM CHECK 16:35 TECHNICIAN\VACUUM CHECK\DRAINING 16:35
1 - COUNTING
2 - DRAINING CHECK VACUUM
Diag.2 Diag.3
ü Check vacuum on the Barflex, vacuum must be around 260mb and must be stable
ü Disconnect the Barflex and connect back the tube on the counting syringe
p. 3/5
VACUUM ADJSUTMENT
COUNTING SYRINGE VACUUM CHECK & ADJUSTMENT
1 - Counting Syringe Vacuum Check

WARNING
ü Disconnect the lowest tube on the counting

syringe and replace it by the Barflex tube (Diag.4)
Pentra 60
Diag.4
ü Enter Menu: Maintenance\Technician\Vacuum Check\Counting\Check (Diag.5&6)
TECHNICIAN\VACUUM CHECK 16:35 TECHNICIAN\VACUUM CHECK\COUNTING 16:35
VACUUM 850
1 - COUNTING
2 - DRAINING
CHECK
Diag.5 Diag.6
ü Check vacuum on the Barflex while the following screen is displayed, vacuum must
be around 220mb
TECHNICIAN\VACUUM CHECK\COUNTING 16:35
VACUUM 850
CHECK VACUUM (220mb)
CHECK
Diag.7
NOTE To get a better adjustment (difference of altitude for example) follow this
procedure
ü Disconnect the Barflex and connect back the tube on the counting syringe
p. 4/5
RAS245B
2 - Counting Syringe Vacuum Adjustment

ü Open the left side panel
ü Disconnect the fourth tube from the top on the counting syringe and replace it by the
Barflex tube (Diag.8)
VACUUM ADJUSTMENT
Diag.8
ü Enter Menu: Maintenance\Technician\Vacuum Check\Counting\Check (Diag.9)

ü Check vacuum on the Barflex, vacuum must be around 220mb
ü Press ESC and as in diagram 9, change the step value. If vacuum is too low
increase step value
TECHNICIAN\VACUUM CHECK\COUNTING 16:35
VACUUM 50
CHECK
Diag.9
ü Repeat vacuum check and step value adjustment until you get the correct vacuum
ü Disconnect the Barflex and connect back the tube
p. 5/5
FRONT PANEL DISMANTLING
• CONCERNS
Instrument dismantling:
Front panel
• REQUIRED TOOLS
Hexagonal keys
Flat screw driver
Torx keys
Pentra 60
30min
• FREQUENCY
On request
None
RAS247B
FRONT PANEL
WARNING
Pentra 60
p. 2/5
RAS247B
FRONT PANEL
1 - Front Panel Dismantling


ü Open the reagent door and remove the 4 bottles (disconnect tube on the stopper)
WARNING
ü Open the cover
WARNING
Fully lift sampling probe before pushing backward sampling carriage
ü Push the carriage back and unscrew the 2 Torx M3x16 screws (several turns) (Diag.1&2)
Diag.1 Diag.2
ü Unscrew the 2 CHC M3x6 screws (Diag.3)
Diag.3
p. 3/5
ü Unscrew the 4 CHC M3x10 screws (Diag.4)
Diag.4
Pentra 60
ü Unscrew the 2 CHC M3x6 screws (just few turns) (Diag.5)
Diag.5
ü Disconnect the flat connector on the LCD & Keyboard board (Diag.6)
Diag.6
ü Lift the front panel and remove it carefully

p. 4/5
RAS247B
2 - Front Panel Mounting

ü Reassemble following steps of chapter 1 backward
ü Run Menu: Maintenance\Autocontrol
ü Check that all is operating normally
p. 5/5
HEATER ASSY REPLACEMENT
• CONCERNS
Replacement of the heater assy
• REQUIRED TOOLS
Hexagonal keys
Flat screw driver
Torx keys
Pentra 60
1h
• FREQUENCY
On request

None
RAS248B
REPLACEMENT OF THE HEATER ASSY
WARNING
Pentra 60
p.2/8
RAS248B
REPLACEMENT OF THE HEATER ASSY
1 - Introduction
ü Heater assy has to be changed only when required

ü To access Heater assy you need to remove the Front panel, follow RAS247 Front
panel dismantling of this manual
ü Pull the Reagents syringe and the LMNE syringe to free space behind (no need to
disconnect tubes) Unscrew several turns the 4 silent block screws of each syring (Diag.1)
Diag.1
p.3/8
2 - Heater Assy Dismantling

ü Unscrew the 2 CHC M3x6 screws shown on next diagram (Diag.2)
Diag.2
Pentra 60
ü Disconnect following tubes on the Chambers assy:
- BASO 2
- RBC 1
- LMNE 2 & 3
- DIL 1
ü The heater assy is free, let it lay on the frame and by the front aperture disconnect
following tubes:
- From valve 22_2 (rep.1 on heater)

- From T4 (rep.5 on heater)
Diag.3
Diag.4
ü The heating system is now as shown on diagram 4
p.4/8
RAS248B
ü Disconnect heating system wiring on the mother board plugged in J31: (Diag.5)
Diag.5
ü Take out the cable through the instrument
ü Take out the heater assy and drain it on absorbant paper
Diag.6
p.5/8
3 - Heater Assy Mounting

ü Take new Heater assy and connect tubes on heater as follow:
NOTE You can put the former and new heater beside and connect following
tubes one to one
REP. ON
TO
HEATER
2 Diluent chamber: DIL 1
4 LMNE chamber: LMNE 3
6 LMNE chamber: LMNE 2
8 RBC chamber: RBC 1
9 rep.11 of the heater

Diag.7
Pentra 60
11 rep.9 of the heater
12 BASO chamber: BASO 2
ü Put new heater, with previous tubing connected, on instrument frame (Diag.8)
ü Connect following tubes: (Diag.9)
REP. ON
TO
HEATER
1 Valve 22 Inlet 2
3 Valve 8 Inlet 2
5 T4
7 Valve 22 Inlet 1
10 Valve 11 Inlet 2
Diag.8 Diag.9
p.6/8
RAS248B
ü Dismantle chamber assy to get the tubes through the chamber’s support (Diag.10)

Diag.10
ü Lift the heater assy, make the 5 tubes go through the chamber’s support steel sheet
(Diag.11)
Diag.11
ü Screw back the 2 heater assy screws (Diag.12)
Diag.12
p.7/8
ü Put back the chamber assy and connect the following tubes on chambers:
- BASO 2
- RBC 1
- LMNE 2 & 3
- DIL 1
ü Connect heating system wiring on the mother board in J33: (Diag.13)

Pentra 60
Diag.13
ü Push back the Reagents syringe and the LMNE syringe and screw silent blocks (check
that no tube is disconnected)
ü Put back the Front panel, follow RAS247 Front panel dismantling of this manual
ü Check chambers position, follow RAS241 Chambers Adjustment of this manual
ü When startup and prime are done check that there is no leak
p.8/8
BUBBLING ADJUSTMENT
• CONCERNS
Bubbling
• REQUIRED TOOLS
None
Pentra 60
15min
• FREQUENCY
On request

None
RAS249B
BUBBLING ADJUSTMENT
BUBBLING ADJUSTMENT
WARNING
Pentra 60
p.2/3
RAS249B
BUBBLING ADJUSTMENT
1 - Introduction
ü Bubbling is factory adjusted and normally does not required further adjustments
ü If an adjustment is necessary follow this procedure
BUBBLING ADJUSTMENT
2 - Bubbling Adjustment
ü Enter Menu: Maintenance\Technician\Bubbling\ (Diag.1)
ü Enter new step value for each bubbling
MAINTENANCE\TECHNICIAN\BUBBLING 16:05
1-AUTOCONTROL CYCLE
FIRST DILUTION 00
LMNE 300
WBC/BASO 300
RBC/PLT 300
ALPHALYSE 400
Diag.1
NOTE When number of steps rises, bubbling is increasing
BUBBLING LOW LIMIT NORMAL HIGH LIMIT
FIRST DILUTION 100 300 400
LMNE 100 300 400
WBC/BASO 100 300 400
ALPHALYSE 300 400 500
p.3/3
POWER SUPPLY REPLACEMENT
• CONCERNS
Power supply
• REQUIRED TOOLS
Hexagonal keys
Flat screw driver
Pentra 60
30min
• FREQUENCY
On request

None
RAS250B
WARNING
Pentra 60
p.2/5
RAS250B

1 - Power supply replacement (1xDBN 004 A)
ü Open the left side panel
ü Open the cover
ü Disconnect , on the mother board, the printer RS flat cable in J2 (Diag.1)
Diag.1
ü With a flat screwdriver disconnect, on the mother board, the supply connector in J37
(Diag.2)
Diag.2
ü Open the left side panel and the main board door (block it)
WARNING
p.3/5
ü Disconnect the Optical bench lamp supply cable (Diag.3)
Diag.3
ü Unscrew the 2 CHC M3x6 back screws of the power supply (Diag.4)
Pentra 60
Diag.4
ü Unscrew the 2 CHC M3x6 front screws of the power supply (Diag.5)
Diag.5
p.4/5
RAS250B
ü Take out the power supply

NOTE Becareful to the lamp power supply cable when you remove the power
supply

ü Check following voltages (Not adjustable):
TEST
DESIGNATION TARGET
POINT
TP40 5V Power supply +5V
Diag.6
p.5/5
START CYCLE MICROSWITCH REPLACEMENT
• CONCERNS
Start cycle microswitch replacement
• REQUIRED TOOLS
Hexagonal keys
Torx keys
Pentra 60
30min
• FREQUENCY
On request

None
RAS251B
START CYLE MICROSWITCH REPLACEMENT
WARNING
Pentra 60
p.2/4
RAS251B

1 - Introduction
ü Start cycle microswitch has to be changed or dismantle only when required
ü To access start cycle microswitch you need to remove the Front panel, follow RAS 247
A Front panel dismantling of this manual
2 - Start Cycle Microswitch Dismantling

ü Remove the thermostated compartment fan (Diag.1)
Diag.2
Diag.1
ü Release the Start cycle microswitch loosening the 2 screws (Diag.2)
Diag.3
ü Disconnect the Start cycle microswitch (Diag.3)
p.3/4
START CYLE MICROSWITCH REPLACEMENT
3 - Start Cycle Microswitch Mounting

ü Connect the new Start cycle microswitch
IMPORTANT
Microswitch is connected Normally Open
ü Put back the Start cycle microswitch in the 2 slots (nearly full backward) (Diag.4)
ü Tighten the 2 microswitch screws
Pentra 60
Diag.4
ü Check with the front panel in its location, that the Start cycle key is operating normally.
If not move forward or backward the microswitch until you get a correct location
NOTE You must ear a «click» when key is pressed, and one when
key is released
ü Reinstall fan
WARNING
Use the washer to connect ground wiring to the fan
ü Follow RAS247 Front panel dismantling of this manual to reassemble front panel
p.4/4
CHECK UP AFTER INTERVENTION
• CONCERNS
Instrument maintenance:
Check up after intervention
• REQUIRED TOOLS
Fresh and normal blood samples
Calibration blood samples
Pentra 60
1h
• FREQUENCY
On request

None
RAS252B
CYCLE CHECK UP AFTER INTERVENTION

Pentra 60
p.2/5
RAS252B
CHECK UP AFTER INTERVENNTION

CYCLE CHECK UP AFTER INTERVENTION
1 - Preliminary
WARNING
This procedure must be performed on a clean instrument
If instrument is suspected to be not perfectly clean, perform an Autoconcentrated
cleaning
WARNING
Disposal gloves and white coat must be worn by the operator
Local or national regulations must be applied in all the operations
ü Run a STARTUP cycle
2 - Repeatability
ü Based on 20 consecutives analyses without alarm from one fresh and normal blood
sample
ü Run 20 consecutives analyses
ü Control to have variation coefficients within the following acceptable limits (Diag.1):
TEST
DESIGNATION TARGET
POINT
Diag.1
X : Mean X=
∑ Xi
Xi : Measure value n
n : Measure number
SD : Standard deviation
SD =
∑ ( X − Xi )²
n −1
ü CV calculated by means of the below formula:
SD
CV (%) = x100
X
ü With one fresh and normal blood sample check that there is no alarm in CBC+5DIFF
mode
p.3/5
3 - Calibration
Autocalibration:
ü Enter Menu: Calibration\Autocalibration\ (Diag.2)
16:05 CALIBRATION 16:05
1 - START CYCLE 1 - AUTOCALIBRATION

2 - CALIBRATION 2 - CHANGE COEFFICIENTS
3 - PRINT COEFFICIENTS
3 - REAGENTS 4 - REPETABILITY
4 - MAINTENANCE
5 - SETUP
Diag.2 Diag.3
Pentra 60
TECHNICIAN \ WBC MATRIX CALIBRATION 16:05

WBC WBC LMNE
1 11.1 11.7
2 11.1 11.7
3 11.2 11.9
CALIBRATION PASSED
PRESS ENTER TO UPDATE COEFFICIENTS
ESCAPE TO RETURN
STAT WBC WBC LMNE
COEFF 0.95
NEW 0.89
MEAN 11.1 11.8
Diag.4
IMPORTANT
Change Lot number, Expiration date or Target values if necessary
Run Calibration:
ü Prepare the calibrator according to the specific instructions (temperature, mixing, etc...)
ü Open the vial and position the sampling needle deeply inside the bottle
ü Press the sampling bar located behind the needle
ü When the cycle LED stops flashing, remove the vial and replace the cap on the calibrator
ü When the analysis cycle ends, the first result is displayed on the result chart table
ü Run the second calibrator sample

WBC WBC LMNE
1 11.1 11.7
2 11.1 11.7
3 11.2 11.9
4
5 CALIBRATION ENDED
PRESS ESCAPE TO EXIT
STAT WBC WBC LMNE

COEFF 0.95
NEW 0.89
MEAN 11.1 11.8
Diag.5
p.4/5
RAS252B
CHECK UP AFTER INTERVENNTION

IMPORTANT
The calibration of the ABX PENTRA 60 can be performed on 3 to 11 analyses. The
autocalculation module performs statistics on these results in order to obtain the
best calibration coefficients.
In order to obtain the best calibration possible, it is recommended to run the calibration
blood a minimum of 5 times.
Calibration passed:
ü If the statistic figures are within the acceptable limits:
- Coefficient of variation is within the limits setup as described section and
- The percentage difference between the target and the mean value is less than 20
ü Press to save the new coefficients (Diag.7)
TECHNICIAN \ WBC MATRIX CALIBRATION 16:05 CALIBRATION\AUTOCALIBRATION 16:05

WBC WBC LMNE WBC RBC HGB HCT PLT
1 11.1 11.7 1 10.0 4.48 13.3 37.5 252
2 11.1 11.7 2 10.1 4.57 13.3 38.0 260
3 11.2 11.9 3 10.1 4.49 13.3 37.5 272
4 11.0 11.8
5 11.1 11.7 ENTER TO UPDATE COEFFICIENTS
ESC TO CONTINUE
STAT WBC RBC HGB HCT PLT
STAT WBC WBC LMNE COEFF 136 209 41.9 222 328
COEFF 0.95 NEW 136 211 41.8 222 316
NEW 0.89 MEAN 10.1 4.51 13.3 37.7 262
MEAN 11.1 11.8 CV% 0.84 1.07 0.23 0.82 3.75
Diag.6 Diag.7
ü Press to print the new coefficients (Diag.8)
CALIBRATION\AUTOCALIBRATION 16:05
WBC RBC HGB HCT PLT
1 9.5 4.92 13.1 43.3 172
2 9.3 4.90 13.0 42.7 171
3 9.5 4.82 13.0 42.2 175
ENTER TO PRINT RESULTS
ESC TO CONTINUE
STAT WBC RBC HGB HCT PLT
COEFF 127 225 41.0 251 225
NEW 136 209 41.9 222 328 H
MEAN 9.4 4.88 13.0 42.7 173
CV% 1.31 1.07 0.32 1.27 1.39
Diag.8
ü Check that the calibration coefficients remain within the following ranges (Diag.9):
Calibration
Standard value minimum maximum
coefficient
WBC 137 90 200
RBC 225 160 290
HGB 40.0 25.0 55.0
HCT 220 160 290
PLT 290 180 400
RDW 0.3 0.1 0.9
Diag.9
p.5/5
STEP BY STEP ANALYSIS CYCLE
• CONCERNS
Instrument control:
Analysis cycle
• REQUIRED TOOLS
Fresh blood samples
Pentra 60
15min
• FREQUENCY
On request

None
RAS253B
STEP BY STEP ANALYSIS CYCLE INTRODUCTION

STEP BY STEP ANALYSIS CYCLE CHECK
WARNING
Pentra 60
p.2/8
RAS253B
STEP BY STEP ANALYSIS CYCLE INTRODUCTION
1 - Introduction
ü The main cycle consists of four principal phases:
1 - Preparation Of The Sample Phase: Dilutions
2 - Count And Measurement Of The WBC Group

3 - RBC/PLT Group Count
4 - Filling Of Tank
ü A special cycle is required when no other cycle is started during RBC/PLT count or
during the filling up of the tank:
5 - Rinsing Of The RBC/PLT Count Chamber
ü All phases are described in this procedure
2 - Cycle Start Condition

ü Run a startup cycle
ü Needle in the sampling position and green LED lit (Diag.1)
ü All chambers (execpt Rinse chamber) are filled with diluent (Diag.2)
ü Reagents syringe & LMNE syringe down (Diag.3)
ü Draining & Counting syringe down (Diag.3&4)
Diag.2
Diag.1
Draining
Syringe
Counting LMNE Reagents

Syringe Syringe Syringe
Diag.3 Diag.4
p.3/8
STEP BY STEP ANALYSIS CYCLE CHECK
1 - Preparation Of The Sample Phase: Dilutions

1 First series of dilutions:
ü Press sampling bar, all the chambers are drained (Draining syringe)
ü Blood sample of 53 µl (Sampling syringe)
ü Transfer of the needle toward the rinsing chamber (Sampling needle motor, Carriage
motor)
ü Distribution of 3 µl of blood during the rinsing of the needle (Sampling syringe, Reagents
syringe)
ü Transfer of the needle from rinse chamber to the first dilution chamber (Sampling needle
motor, Carriage motor)
ü Distribution of 10 µl of blood into a flux of 1.7 ml of diluent (Sampling syringe, Reagents

Pentra 60
syringe)
ü Transfer of the needle from the first dilution chamber to the WBC and BASO count
chamber (Sampling needle motor, Carriage motor)
ü Distribution of 10 µl of blood into a flux of 2 ml of BASOLYSE ll (Sampling syringe,

Reagents syringe)
ü Transfer of the needle from the WBC and BASO count chamber to the LMNE mixing
ü Distribution of 25 µl of blood into a flux of 1 ml of EOSINOFIX (Sampling syringe, Reagents

syringe)
ü Transfer of the needle from the LMNE mixing chamber to the rinse chamber (Sampling
needle motor, Carriage motor)
ü Distribution of the 5 µl of residual blood during the double rinsing (interior and exterior) of
the needle (Sampling syringe, Reagents syringe)
NOTE Realisation of the LMNE dilution:

The dilution in the LMNE chamber is completed by 1 ml of diluent after 12
seconds of incubation:
ü Positioning of the needle opposite the distribution nipple for the
EOSINOFIX
ü Control of valve 8 then set the syringe group for a dose of 1 ml
ü Simultaneously: Setting of the distribution syringe to distribute 25 µl of
blood
ü Stop valve 8
ü Incubation for 12 seconds
ü Control of valves 1 and 3 and setting of the LMNE injection syringe to
distribute 1 ml of the stop diluent
ü Stop valves 1 and 3
p.4/8
RAS253B
2 Second dilution for the RBC and the PLT:

ü Transfer of the needle from the rinse chamber to the first dilution chamber (Sampling
needle motor, Carriage motor)
ü Sample of 42.5 µl of the first dilution (Sampling syringe)

ü Rinsing of needle exterior (in the first dilution chamber) with 0.4 ml of diluent (Reagents
syringe)
ü Transfer of the needle from the first dilution chamber to the RBC and PLT count
ü Distribution of the 42.5 µl of first dilution into a flux of 2 ml of diluent (Sampling needle
motor, Carriage motor)
ü Distribution of 0.5 ml of diluent from the interior of the needle (Sampling needle motor,
Carriage motor)
ü In the case of this RBC/PLT dilution, it is the entire blood sample that
NOTE is distributed (and not a portion as in the preceding dilution). The distri-
bution in a flux is kept in order to benefit from the homogenisation as-
pect of the procedure
ü In order to attain the hemoglobin measurement we add 0.4 ml of

lysing agent into the first dilution chamber immediately following the
blood sample for the RBC/PLT dilution1
p.5/8
2 - Count And Measurement Of The WBC Group

ü LMNE:
Loading of the vacuum injection circuit of the optical bench (Draining syringe, control of
valves 4 and 5)
ü Start flux in the optical chamber (approximately 2 seconds)
ü Injection, acquisition of the matrix and WBC count for 12 seconds
ü Simultaneously: WBC and BASO counts:

Rinsing of the counting head (Diluter pushed into position, control of valves 9, 10, 21, 23
and 13)
ü Start the vacuum and first part of count (duration of 6 seconds, control of valve 23)
ü Rinsing of counting head and adjustment of the vacuum count
ü Second part of count (duration of 6 seconds)

Pentra 60
ü After this first series of measurements:

Measurement of the hemoglobin
Rinsing of the WBC/BASOS count chamber with a mixture of 1.06 ml of ABX UNICLEAN and
1.44 ml of diluent ABX DIL
Rinsing of the WBC/BASOS counting head
p.6/8
RAS253B
3 - RBC/PLT Group Count

ü Rinsing of the RBC/PLT counting head
Start the vacuum for the RBC/PLT counting head
ü First series of counts (duration of 5 seconds, control valve 14).
ü Rinsing of the RBC/PLT counting head and adjustment of the vacuum.

ü Second series of counts (duration of 5 seconds)
ü P.R.F. Process (Permanent Rinse Flow)

NOTE The WBC/BASOS and the RBC/PLT counting heads are «in series» during
the RBC/PLT count. The count flux from the WBC chamber circulates in
the RBC/PLT count head and limits the possibility of the circulation of the
RBC behind the count orifice.
ü Autocleaning of the WBC ruby

The P.R.F. allows as well an auto cleaning of the WBC/BASOS inasmuch
as a UNICLEAN/DIL (cleaning solution) mixture is drawn through the WBC/
BASO ruby during the entire duration of the RBC and PLT counts
4 - Filling Of Tank
ü At the end of the RBC/PLT count, the RBC count vacuum is «finished» and used for the
filling up of the diluent tank (control valves 15 and 25)
ü In order to improve the filling speed, the syringe is initialised for a few seconds then drawn
again to complete the filling
ü A float located in the tank controls the level of liquid
p.7/8
5 - Rinsing Of The RBC/PLT Count Chamber

ü For reasons of maximising the mechanical resources, the rinsing of the RBC/PLT chamber
is «normally» programmed for the beginning of the succeeding cycle (which can be started
during the RBC and PLT count)
ü If no other cycle is started during RBC/PLT count or during the filling up of the tank, a
special rinsing sequence is initiated. The sample signal light changes to red and the instru-
ment is «jammed» for a few seconds
ü This sequence involves draining of the RBC chamber followed by a refilling with the diluent
Pentra 60
p.8/8
OPTICAL BENCH REPLACEMENT
• CONCERNS
Dismantling, Replacement and Control of Optical Bench
• REQUIRED TOOLS
Hexagonal keys
Flat screw driver
Torx keys
Pentra 60
30min
• FREQUENCY
On request

None
RAS254B
DISMANTLING & REPLACEMENT OF THE OPTICAL BENCH

OPTICAL BENCH CONTROL
WARNING
Pentra 60
p.2/6
RAS254B
DISMANTLING & REPLACEMENT OF THE OPTICAL BENCH
1 - Optical Bench Dismantling


WARNING
ü Open the cover

ü Disconnect tubes from LMNE syringe:
- Inlet 1
- Inlet 5
ü Disconnect tubes from valves:
- 1 Inlet 1
- 4 Inlet 2
ü Disconnect tube from ground fitting (Diag.1)
Diag.1
ü Disconnect following connections: (Diag.2)

- Lamp supply
- LMNE CIS & LMNE OD coaxes
- Optical pre-ampli board supply
- Optical bench grounding wire
Diag.2
p.3/6
ü Remove gently the Optical bench

ü Untighten the silent blocks (5mm allen key) (Diag.3)
Diag.3
Pentra 60
2 - Optical Bench Replacement

ü Locate new Optical bench
ü Tighten the silent blocks (5mm allen key) (Diag.3)
NOTE Do not tighten silent blocks too hard, check that optical bench Cannot be
lifted
ü Connect all tubing and wiring as described in Optical Bench Dismantling
p.4/6
RAS254B
OPTICAL BENCH CONTROL
1 - Emission Gun Location Control

ü Open Optical bench cover (Diag.4)

Diag.4
ü Check that gap between emission gun and flowcell is around 3mm (Diag.5)
WARNING
Becareful, if you use a 3mm allen key to control gap, that you don’t damage flowcell or
emission gun
Diag.5
WARNING
This adjustment is factory made and must not be changed
p.5/6
2 - Optical Bench Lamp Alignement Control

ü Open Optical bench cover (Diag.6)
Diag.6
ü To control lamp put a piece of white paper between reception gun and flowcell (Diag.7)
Pentra 60
Diag.7
ü Check that projection of lamp filament is vertical (Diag.8)
Diag.9
Diag.8
WARNING
A small screw (1.2mm allen key) allows adjutment of lamp (Diag.9)
This adjustment is factory made and must not be changed
p.6/6
BARCODE READER CONFIGURATION
• CONCERNS
Barcode Reader test and configuration
• REQUIRED TOOLS
None
Pentra 60
15min
• FREQUENCY
On request

None
RAS255B
BARCODE READER TEST
WARNING
Pentra 60
p.2/6
RAS255B

BARCODE READER TEST
1 - Test
ü Check that barcode reader is working properly by means of the reading test (Diag.1)
READ TEST for DATALOGIC
WITH CHECKSUM WITHOUT CHECKSUM

EAN 8 C39
1234567 TEST
EAN 8 2/5
9076543210
1234567
CODABAR
EAN 13
543210
123456789012
C128
EAN 13
0123456789
123456789012
Diag.1
ü If the reading test is correct, the procedure is completed
p.3/6
2 - Procedure to carry out in case of reading test failure

ü Switch on the instrument and within the 15 first seconds, read from the top to the bottom the
following barcode labels on this page. The audible beep occurs after each reading. Read all the
labels from top to bottom and from left to right
ü When the last label is read, check once more on the test labels (Diag.1)
WARNING
For code I2 of 5, to avoid a bad interpretation, it is mandatory to hold the reader in
order to read the entire code
ü If the reading test has failed for the DATALOGIC reader, proceed to the barcode setup as
follows:
Pentra 60
$+CQ0$- AB11AB*0116 EB3EHB54FF
$+CP0$- AC110416 EB3EHNFFFF
$+$* AI11 EB3EHVFFFF
$+ AD111AD*0316 EB3EHRFFFF
CA0CC1CE2 EA110DEC0 EB3EHTFFFF
AZ0AA3 EB3EHA45FF $-
p.4/6
RAS255B

DATALOGIC M6165-M0 READER TEST AND SETUP
ü Make sure that the BARCODE reader is properly fitted in the DIN plug of the RS adaptor
wiring.
ü Check the barcode reader works properly by means of the reading test on this page. If the
reading test is correct, the procedure is completed. If not, continue below.
Procedure to carry out in case of reading test failure
If the reading test fails, proceed as follows:
ü Switch on the instrument and within the 15 first seconds, read from the top to the bottom the
barcode labels located on page 4 of this procedure. The audible beep occurs after each reading.
Read all the labels from top to bottom and from left to right.
ü When the last label is read, the signal beeps 5 times in order to indicate the end of the setup.
Check once more on the test labels.
WARNING
For code I2 of 5, to avoid a bad interpretation, it is mandatory to hold the reader in
order to read the entire code
C39
TEST
2/5
9076543210
CODABAR
543210
C 128
98765
p.5/6
DATALOGIC DLC 6165-M0 BARCODE READER SETUP
$+CQ0$- Barcode module output mode
$+CP0$- RS232 interface
$+$* Restore system default configuration
$+ Enter configuration environment
CA0CC1CE2 7 data bits, parity even, XON/XOFF

Pentra 60
AZ0 Disable all family codes
AB11AB*0ll6 Code39, no C.D. , 1 to 16 char
AC110416 ITF, no C.D. , 4 to 16 char
AD111AD*0316 Codabar, no start/stop, 3 to 16 char
AI11 Code128, C.D. control without transmission
EA110DEC0 Terminator = CR, FIFO disabled
ED3 4 good reads before accepting code
$- Exit and save configuration
p.6/6
LMNE BALANCE ADJUSTMENT
• CONCERNS
Adjustment of the LMNE Balance.
• REQUIRED TOOLS
None
Pentra 60
None
15 min
• FREQUENCY
On request

None
RAS304B
INTRODUCTION
AUTO-CALIBRATION
FORCED CALIBRATION
WARNING
Pentra 60
p.2/5
RAS304B
INTRODUCTION

WARNING
This procedure must be performed on a clean instrument.
If the instrument is suspected to not be perfectly clean, perform a Concentrated
cleaning.
ü Start the Analyzer.
ü Run a startup (If not performed automatically).
AUTO-CALIBRATION
ü Enter menu «Maintenance\Technician\WBC Matrix Calibration» (Diag.1):

WBC WBC LMNE
RUN AT LEAST 5 SAMPLES

AND PRESS ENTER TO CALIBRATE
STAT WBC WBC LMNE
COEFF 0.95
NEW
MEAN
Diag.1
ü Prepare the sample according to the specific instructions (temperature, mixing, etc...),
insert the tube and close the tube holder.
IMPORTANT
Risk of erroneous results if the calibrator is not continously mixed between each
analysis. Continue mixing the calibrator between each analysis.
ü When the analysis cycle ends, the first result is displayed on the result chart table.
ü When the Green LED turns on, run the same specimen again.
IMPORTANT
At least 5 results are necessary to calibrate the LMNE Balance.
p.3/5
ü The results selected to be involved in the statistical calculation have a dot in the square
column. To discard a result from the statistical calculation, select the line and press the
DOT key (Diag.2):

WBC WBC LMNE
1 11.1 11.7
2 11.1 11.7
3 11.2 11.9
4 11.0 11.8
5 11.1 11.7
STAT WBC WBC LMNE

COEFF 0.95
NEW 0.89
MEAN 11.1 11.8
Diag.2
ü When ready to calibrate press ENTER to apply new calibration coefficient (Diag.3):

Pentra 60
WBC WBC LMNE

1 11.1 11.7
2 11.1 11.7
3 11.2 11.9
CALIBRATION PASSED
PRESS ENTER TO UPDATE COEFFICIENTS
ESCAPE TO RETURN
STAT WBC WBC LMNE
COEFF 0.95
NEW 0.89
MEAN 11.1 11.8
Diag.3
ü Enter user password to confirm calibration (Diag.4):

WBC WBC LMNE
1 11.1 11.7
2 11.1 11.7
3 11.2 11.9
4
5
USER PASSWORD
*
STAT WBC WBC LMNE
COEFF 0.95
NEW 0.89
MEAN 11.1 11.8
Diag.4
ü When calibration is ended press ESCAPE to exit (Diag.5):

WBC WBC LMNE
1 11.1 11.7
2 11.1 11.7
3 11.2 11.9
4
5 CALIBRATION ENDED
PRESS ESCAPE TO EXIT
STAT WBC WBC LMNE

COEFF 0.95
NEW 0.89
MEAN 11.1 11.8
Diag.5
p.4/5
RAS304B
ü The calibration values are printed out (Diag.6):

Diag.6
FORCED CALIBRATION
ü Enter menu «Calibration\Change coefficeint» (Diag.7):
CALIBRATION\CHANGE COEFFICIENT 16:05
WBC 131
HCT 218
RBC 251
HGB 41
PLT 232
RDW 0.50
MPV 1.00
WBC LMNE 0.95
Diag.7
ü With Up and Down arrows select the WBC LMNE calibration coefficient and change it.
p.5/5
TRAINING SLIDES
EXPLODED VIEWS
1. 11 Valves assy ............................................................................ 1
2. 5 Valves assy (12-16) ................................................................... 2
3. 2 Valves assy .............................................................................. 3
4. 7 Valves assy .............................................................................. 4
5. 5 Valves assy (27-31) ................................................................... 5
6. Optical bench syringe assy ......................................................... 6
7. Reagents syringe assy ................................................................ 7
8. Sampling syringe assy ................................................................. 8
9. Syringe motorization carriage assy ............................................... 9
10. Syringe motor assy ...................................................................... 10
11. Syringe motor body assy ............................................................. 11
12. Sampling motor assy ................................................................... 12
13. Optical syringe motor assy .......................................................... 13
14. Reagents motor assy ................................................................... 14
15. Sampling syringe assy ................................................................. 15
16. Vacuum syringe piston assy ........................................................ 16
17. Vacuum syringe assy ................................................................... 17
18. Short vacuum syringe assy .......................................................... 18
19. Long vacuum syringe ................................................................... 19
20. Long vacuum syringe assy .......................................................... 20
21. Short vacuum syringe assy .......................................................... 21
22. Needle carriage motor assy ......................................................... 22
23. Horizontal sampling carriage notched belt ................................... 23
24. Notched belt ................................................................................ 24
25. Needle carriage assy ................................................................... 25
26. Sampling horizontal carriage ........................................................ 26
27. Sampling horizontal carriage assy ................................................ 27
28. Sampling carriage assy ............................................................... 28
29. Carriage vertical belt .................................................................... 29
30. Carriage rinsing block assy .......................................................... 30
31. Home carriage cell ....................................................................... 31
32. Needle assy ................................................................................ 32
33. Carriage motor assy ..................................................................... 33
34. Sampling needle assy ................................................................. 34
35. Reagents heating coils assy ........................................................ 35
36. Thermostated room assy ............................................................. 36
37. Thermostated room plate assy .................................................... 37
38. Thermostated room fan assy ....................................................... 38
39. Frame assy .................................................................................. 39
40. Diluent chamber assy .................................................................. 40
41. Diluent chamber .......................................................................... 41
42. RETIC flowcell assy ..................................................................... 42
43. Optical bench assy ..................................................................... 43
44. Optical bench lamp ..................................................................... 44
45. Preamplifier support .................................................................... 45
46. Cover pentra 60 assy .................................................................. 46
47. LED board pentra 60 assy ........................................................... 47
48. Pentra 60 chamber assy .............................................................. 48
49. Pentra 60 chamber assy .............................................................. 49
50. WBC/HGB/BASO Chamber assy ................................................. 50
SPARE PART LIST
REF DESIGNATION PACKAGING CATEGORY
CAE006A SWITCH,MICROSWITCH XC5-81-82 SINGLE USE
CAE010A SWITCH,MICROSWITCH XC5-81 SINGLE USE
DAC011A CABLE,POWER EUROPE SINGLE USE
DAC012A CABLE,POWER USA SINGLE USE
DAJ007A LAMP,ASSY FOR OPTIC BENCH P60 CONSUMABLE
DBH001A TYRAP,SMALL PACK OF 1000 CONSUMABLE
DBH002A TYRAP,BIG PACK OF 1000 CONSUMABLE
DBK004A ADHESIVE,HOLDER TYRAP D=3MM PACK OF 10 CONSUMABLE
DBN004A PCB,POWER SUPPLY BLOCK P60 STD EXCH
EAC008A FITTING,ANTI ROTATION WASHER PACK OF 5 CONSUMABLE
EAC010A FITTING,LUER FEMALE I=3MM PACK OF 5 CONSUMABLE
EAC019A FITTING,LUER MALE I=3MM PACK OF 5 CONSUMABLE
EAE005AS TUBING,TYGON 1,016MM (0,040) PACK OF 2M CONSUMABLE
EAE011AS TUBING,CRYSTAL 3X6 PACK OF 10M CONSUMABLE
EAE028AS TUBING,CRYSTAL 4X6 PACK OF 2M CONSUMABLE
EAE034AS TUBING,TYGON 2.54MM (0,100) PACK OF 2M CONSUMABLE
EAE051AS TUBING,SILICONE 4,5 X 8 PACK OF 2M CONSUMABLE
FAA040A O’RING,5DIFF SYRINGE D=12,1 PACK OF 10 CONSUMABLE
FAA046A O’RING,COAXIAL CABLE PACK OF 10 CONSUMABLE
FAA053A O’RING,SAMPL. NEEDLE MICROS OT PACK OF 10 CONSUMABLE
FAA064A O’RING,SAMPLING SYRINGE P60 PACK OF 10 CONSUMABLE
FAA065A O’RING,REAGENT SYRINGE D=6,3 PACK OF 10 CONSUMABLE
FAA066A O’RING,DRAINING CHAMBER P60 PACK OF 12 CONSUMABLE
FAA067A O’RING,5DIFF SYRINGE D=2,4 PACK OF 10 CONSUMABLE
FAK001A CHAMBER,APERTURE 50µ CONSUMABLE
FAK003A CHAMBER,APERTURE 80µ CONSUMABLE
FAL009A SILENT BLOC,FOR SUB ASSEMBLIES PACK OF 12 CONSUMABLE
FAL010A SILENT BLOC,OPTIC BENCH P60 PACK OF 12 CONSUMABLE
FAM004A COVER,PLASTIC FOOT FOR CHASSIS PACK OF 8 CONSUMABLE
FBH016A COVER,DUST COVER PENTRA60 CONSUMABLE
FBL001A REAGENT,REAGENT CAP 2 HOLES CONSUMABLE
FBR011A BELT,NEEDLE L=364 P60 CONSUMABLE
FBR012A BELT,CARRIAGE L=544 P60 CONSUMABLE
FZZ024A DOOR,PUSH PUSH P60 PACK OF 3 CONSUMABLE
GAK302A REAGENT,BOTTLE STOPPER D=40 PACK OF 12 CONSUMABLE
GAL094A CHAMBER,WAST.P120/DIL TANK P60 SINGLE USE

GBC015A CLIP,MIX CH. HOLDER MIC/P120 PACK OF 10 CONSUMABLE
GBC030A SYRINGE,REAGENT PISTON MIC/P60 SINGLE USE
GBC031A SYRINGE,LYSE PISTON MIC/P60 SINGLE USE
GBG003A CHAMBER,DRAINING 1/4 TURN P60 SINGLE USE
GBG007A CHAMBER,DRAINING CHICANE P60 PACK OF 4 CONSUMABLE
GBG013A CHAMBER,OVERFLOW CUP T° ROOM CONSUMABLE
GBG022A CHAMBER,T° ROOM COVER P60 SINGLE USE
GBG023A PROTECTION,FOR VALVES T° ROOM CONSUMABLE
GBG033A SYRINGE,REAGENT BLOCK BODY P60 SINGLE USE
GBG037A SYRINGE,5DIFF BLOCK BODY P60 SINGLE USE
GBG040A SYRINGE,5DIFF PISTON P60 SINGLE USE
GBG042A SYRINGE,5DIFF CROSSPIECE P60 CONSUMABLE
GBG044A SYRINGE,SAMPLING SYR. BODY P60 SINGLE USE
GBG048A SYRINGE,SAMPLING CROSSPIECE CONSUMABLE
GBG052A SYRINGE,VAC/WASTE PISTON P60 SINGLE USE
GBG053A SYRINGE,VACCUM PUMP BODY P60 SINGLE USE
GBG054A SYRINGE,WASTE PUMP BODY P60 SINGLE USE
GBG090A CARRIAGE,RINSING BLOCK P60 SINGLE USE
GBG091A CARRIAGE,NEEDLE GUIDE P60 SINGLE USE
GBG093A MOTOR,PULLEY P60 CONSUMABLE
GBG096A KEYBOARD,SILICON SINGLE USE
GBG100A STICKER,FRONT PANEL P60 CONSUMABLE
GBG130A COVER,TOP COVER P60 COVER
GBG137A COVER,LEFT SIDE COVER P60 COVER
GBG138A DOOR,KEY FOR T° ROOM P60 SINGLE USE
GBG144A REAGENT,STRAW D=27 P60 ACCESSORIES
GBG145A REAGENT,STRAW D=20 P60 ACCESSORIES
GBG147A CUP,OVERFLOW REAGENTS SYR. P60 CONSUMABLE
GBG155A REAGENT,BOTTLE STOPPER D=25 PACK OF 4 CONSUMABLE
GBG156A O'RING,APERTURE P60 PACK OF 12 CONSUMABLE
GBG157A CHAMBER,COUNTING HEAD P60 SINGLE USE
HAE026B STICKER,VALVES JOUCO (1-10) PACK OF 10 CONSUMABLE
HAW002A STICKER,FOR REAGENT DOOR P60 CONSUMABLE
MAB090A TOOL,TORX KEY T10 TOOL
NAA068A STICKER,FOR TUBING (169 ITEMS) CONSUMABLE
RAA016A MANUAL,TECHNICAL PENTRA60 TOOL

RAB080A MANUAL,USER’S PENTRA60 GB TOOL
RAC046A MANUAL,USER’S PENTRA60 FR TOOL
RAP012A MANUAL,SPLIT VIEWS PENTRA60 TOOL
XAA398A PRINTER,EPSON LX300 220V PERIPHERAL
XAA399A PRINTER,EPSON LX300 110V PERIPHERAL
XAA423BS PCB,PREAMP+MECA ASSY PENTRA60 STD EXCH
XAA425BS PCB,MAIN BOARD PENTRA60 STD EXCH
XAA427BS PCB,LINK BOARD PENTRA60 STD EXCH
XAA429A PCB,LED BOARD FOR COVER P60 STD EXCH
XAA444A PCB,LCD SCREEN EQUIPPED P60 STD EXCH
XBA144A CABLE,REAGENT GROUND FITTING CONSUMABLE
XBA193A CABLE,COAX BASO-O.D LMNE-LMNE CONSUMABLE
XBA386A CABLE,T° ROOM HARNESS PENTRA60 SINGLE USE
XBA389A PHOTOMETER,HB CPTE PENTRA60 SINGLE USE
XBA390A MOTOR,FOR SYRINGE BLOCKS P60 SINGLE USE
XBA391A MOTOR,CARRIAGE PENTRA60 SINGLE USE
XBA392A MOTOR,SAMPLING NEEDLE PENTRA60 SINGLE USE
XBA393A FAN,MAIN FAN 24V PENTRA60 SINGLE USE
XBA394A SENSOR,CARRIAGE MOVE PENTRA60 SINGLE USE
XBA395A SENSOR,DRAINING DETECTION P60 SINGLE USE
XBA396A SENSOR,SAMPL. NEEDLE MOVE P60 SINGLE USE
XBA398A CABLE,COAX RBC/WBC PENTRA60 CONSUMABLE
XBA399A CABLE,COAX LMNE FLOWCELL P60 CONSUMABLE
XBA403A TUBING,SHIELD REPARTITOR P60 CONSUMABLE
XCA166A CHAMBER,ISOLATOR (LONG) CONSUMABLE
XCA167A CHAMBER,ISOLATOR (SMALL) CONSUMABLE
XDA481B VALVE,LIQ. 2WAYS/NC W/O COIL SINGLE USE
XDA483B VALVE,LIQ. 3WAYS W/O COIL SINGLE USE
XDA555AS TOOL,FLOWCELL ADJ. KNOB TOOL
XDA591AS SYRINGE,5DIFF ASSY PENTRA60 SINGLE USE
XDA592AS SYRINGE,REAGENT ASSY PENTRA60 SINGLE USE
XDA593AS SYRINGE,SAMPLING ASSY P60 SINGLE USE
XDA594AS MOTOR,BLOCK FOR 5DIFF SYR. P60 SINGLE USE
XDA595AS MOTOR,BLOCK FOR REAG. SYR. P60 SINGLE USE
XDA596AS MOTOR,BLOCK FOR SAMPL. SYR.P60 SINGLE USE
XDA597AS SYRINGE,WASTE COMPLETE P60 SINGLE USE
XDA598AS SYRINGE,VACCUM COMPLETE P60 SINGLE USE
XDA600A OPTICAL,BENCH PENTRA60 STD EXCH
XDA601AS CHAMBER,LMNE FLOWCELL PENTRA60 SINGLE USE

XDA602A CHAMBER,4 CHAMBERS BLOCK P60 SINGLE USE
XDA605A CHAMBER,DILUENT TANK ASSY P60 SINGLE USE
XDA610A CHAMBER,WBC/BASO CPTE PENTRA60 SINGLE USE
XDA611CS VALVE,LIQ 11 VALVE ASSY (1-11) SINGLE USE
XDA616AS NEEDLE,190µL 5DIFF SYR. P60 CONSUMABLE
XDA617AS NEEDLE,100µL SAMPLING SYR. P60 CONSUMABLE
XDA618AS CARRIAGE,NEEDLE CARRIAGE P60 SINGLE USE
XDA619AS NEEDLE,SAMPLING NEEDLE P60 CONSUMABLE
XDA621A O'RING,VAC/WASTE PUMP + WASHER PACK OF 10 CONSUMABLE
XDA622A O'RING,REAGENT SYR. + WASHER PACK OF 10 CONSUMABLE
XDA624AS DOOR,FOR T° ROOM EQUIPPED P60 SINGLE USE
XDA625AS HEATER,BLOCK COMPLETE PENTRA60 SINGLE USE
XDA626AS CHAMBER,DILUENT TANK COVER P60 SINGLE USE
XDA627AS PCB,KEYBOARD/LCD DISP ASSY P60 STD EXCH
XDA628AS COVER,FRONT COVER EQUIPED P60 SYSTEM COVER
XDA629AS COVER,REAGENT DOOR EQUIPED P60 SYSTEM COVER
XEA018A REAGENT,STRAW DILUENT L=360 CONSUMABLE
XEA019A GREASE,SILICON CONTAINER CONSUMABLE
XEA286AS KIT,O'RING + WASHER P60/P120 CONSUMABLE
XEA311AS KIT,FITTINGS CONSUMABLE
XEA484A KIT,INSTALLATION PENTRA60 CONSUMABLE
XEA485AS KIT,MAINTENANCE P60 6 MONTHS CONSUMABLE
XEA811AS KIT,MAINTENANCE P60 1 YEAR CONSUMABLE
XEA487AS KIT,T° ROOM FAN + HARNESS P60 SINGLE USE
XEA488AS KIT,HEATER HARNESS P60 SINGLE USE
XEA581BS KIT,SYRINGE PISTONS KIT P60 SINGLE USE

Abx (P60)

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Pentra 60

INDEX P/N REVISION REVISION SECTION DATE

A Creation all 19/07/99

B RAN062A Update all 25/05/04

1. Technical specifications ..................................................................................... 1-2

2. Physical specifications ....................................................................................... 1-6

3. Summary of performance data ........................................................................... 1-9

4. Reagents specifications ................................................................................... 1-15

5. Limitations ........................................................................................................ 1-16

2-HYDRAULIC & PNEUMATIC PRINCIPLES

2. Pneumatic diagrams .......................................................................................... 8

3-ELECTRIC & ELECTRONIC PRINCIPLES

2. Pentra 60 Boards ..................................................................................................... 7

4. Pentra 60 Synoptic ................................................................................................ 24

4-ANALYSIS CYCLE TECHNOLOGY

2. Measuring principles .......................................................................................... 4

2. Software Overview: Technician Menu Overview ................................................ 3

3. Software Release ............................................................................................... 4

7-ALARMS & ERROR LIST

4. Technical Operations .......................................................................................... 3

2. Maintenance kits ................................................................................................ 4

1. Blowups views .................................................................................................... 1

11-SPARE PART LIST

1. Spare part list ...................................................................................................... 1

1. TECHNICAL SPECIFICATIONS ................................................. 1-2

1.1.1. CBC Mode

WBC White Blood Cell

RBC Red Blood Cell

1.1.2. CBC + 5DIFF Mode

WBC White Blood Cell

RBC Red Blood Cell

MCH MCHC RDW MPV PCT PDW

LYC LYC MON MON NEU NEU EOS

EOS BAS BAS ALY ALY LIC LIC

1.2. Throughput Analyses

1.3. Tube Identification

1.5. Keyboard & Display

1.6. Data processing

1.7. Measurements and computation

2.1. Power requirements

• Power Consumption: ......... * Analyzer: 200 VA

2.2. Operating temperature and humidity

2.3. Dimensions and Weight

2.4. Minimum specimen volume

2.5. Dilution ratios

2.6. Hgb measurement

• Light source ....................... * Electroluminescent diode

2.7. Counting aperture diameters

2.8. Reagent consumption (ml)

* for one background count only (maxi = 3)

3. SUMMARY OF PERFORMANCE DATA

PARAMETER ABX MINOTROL WITHIN RUN SD OF RUN SD OF DAILY TOTAL

*Source: 510K submission K030144

PARAMETER % CV NOMINAL VALUES

*Source: 510K submission K030144

3.2. Precision (Repeatability)

PARAMETERS %CV TEST LEVEL