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    Lecture Notes
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    The document lists different staining errors that can occur when preparing peripheral blood smears, including being too acidic or too basic. Errors in the acidic direction include using a solution that is too acidic, an excess of buffer or stain, insufficient staining time, or a thin smear. Errors in the basic direction include using an alkaline solution, too little buffer, excessive staining time, inadequate washing, a short drying period, or an old or thick smear.

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    Hematology Notes

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    The document lists different staining errors that can occur when preparing peripheral blood smears, including being too acidic or too basic. Errors in the acidic direction include using a solution that is too acidic, an excess of buffer or stain, insufficient staining time, or a thin smear. Errors in the basic direction include using an alkaline solution, too little buffer, excessive staining time, inadequate washing, a short drying period, or an old or thick smear.

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    © All Rights Reserved
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    12 views1 page

    Peripheral Blood Examination

    Uploaded by

    Lecture Notes
    The document lists different staining errors that can occur when preparing peripheral blood smears, including being too acidic or too basic. Errors in the acidic direction include using a solution that is too acidic, an excess of buffer or stain, insufficient staining time, or a thin smear. Errors in the basic direction include using an alkaline solution, too little buffer, excessive staining time, inadequate washing, a short drying period, or an old or thick smear.

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    List down the different staining errors (too acidic/too basic) that are encountered in peripheral blood

    smear preparation.

    Too Acidic Too Basic


    • Too acid or buffer or stain solution (pH • Too alkaline buffer or stain solution
    below 6.4) (pH above 6.8)
    • Excess buffer or stain solution • Too little buffer for stain solution
    • Insufficient staining time • Excessive staining time
    • Very thin smear • Inadequate washing
    • Contaminants (e.g. Chlorine) in wash • Short drying period
    water • Wash water too alkaline
    • Exposure of buffer or stain solution to • Thick smears
    acid fumes • Old smear (dried plasma produces
    • Old stain in which methanol has oxidized blue background)
    to fumic acid • Protein abnormality (e.g. Multiple
    myeloma)
    • Heparin blood sample
    • Very high leukocyte count with many
    blasts
    • Low hematocrit

    Reference:

    Lotspeich-Steininger, C. A., Stiene-Martin, E.A., Keopke, J.A. (1992). Clinical Hematology


    Principles, Procedures, Correlations. J.B. Lippincott Company. Page 34.

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