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Rev chil estud med (2005); 4(1):40
el efecto alguicida del sobrenadante total, del las proteinas
del sobrenadante concentradas y del sobrenadante
desproteinizado,
Caracterizacion del efecto alguicida.Cytophaga sp
4) Determinacién las actividades. enzimiticas presentes
nel sobrenadante del cultivo bacteriano (ApiZym).
El sobrenadante de Cyrophaga que contenia 0,9280
mg/ml de proteinas, presenté las actividades enzimticas
Setlaladas en la Tabla 1, El SFD completo presenta actividad:
Fosfatasa alcalina, Esterasa, Esterasa lipasa,Lipasa,Leucina
arilamidasa, Fosfatasa dcida,Naftol AS-BI- fosfohidrolasa.
Las protefnas concentradas presentan las mismas
actividades con mayor intensidad colorimétrica excepto
lipasa que estéausente. En el SFD hervido durante 7 minutos
persiste actividad fosfatasa alcalina, Esterasa lipasa,
Fosfatasa dcida,Naftol AS BI fosfohidrolass, Tras
desproteinizar persisten en el sobrenadante tratado con
sulfato de amonio las actividades fosfatasaalcalina, Esterasa
lipasa, Naftol AS BI fosfohidrolasa. Cabe destacar que en
todas las pruebas enziméticas se mantiene la actividad 12,
siendo ésta: Naftol -AS- BI fosfohidrolasa.
b) Electroforesis unidimensional en condiciones
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