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    The Differences Between HPLC GC

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    HPLC and GC are chromatography methods that separate molecules based on their interactions with a mobile phase and column. HPLC uses a liquid mobile phase while GC uses gas, and HPLC columns are longer metal or glass tubes tightly packed with silica or carbon chains compared to coiled GC columns with coated interior walls. HPLC is better for less volatile samples that may contain salts or charges, while GC is used for more volatile compounds and allows for temperature control within its oven.

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    The Differences Between HPLC GC

    Uploaded by

    Sh_Pharmacy
    732 views1 page
    HPLC and GC are chromatography methods that separate molecules based on their interactions with a mobile phase and column. HPLC uses a liquid mobile phase while GC uses gas, and HPLC columns are longer metal or glass tubes tightly packed with silica or carbon chains compared to coiled GC columns with coated interior walls. HPLC is better for less volatile samples that may contain salts or charges, while GC is used for more volatile compounds and allows for temperature control within its oven.

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    The Differences Between HPLC gc

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    HPLC and GC are chromatography methods that separate molecules based on their interactions with a mobile phase and column. HPLC uses a liquid mobile phase while GC uses gas, and HPLC columns are longer metal or glass tubes tightly packed with silica or carbon chains compared to coiled GC columns with coated interior walls. HPLC is better for less volatile samples that may contain salts or charges, while GC is used for more volatile compounds and allows for temperature control within its oven.

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    732 views1 page

    The Differences Between HPLC GC

    Uploaded by

    Sh_Pharmacy
    HPLC and GC are chromatography methods that separate molecules based on their interactions with a mobile phase and column. HPLC uses a liquid mobile phase while GC uses gas, and HPLC columns are longer metal or glass tubes tightly packed with silica or carbon chains compared to coiled GC columns with coated interior walls. HPLC is better for less volatile samples that may contain salts or charges, while GC is used for more volatile compounds and allows for temperature control within its oven.

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    The Differences Between HPLC & GC

    o HPLC (high performance liquid chromatography) and GC (gas chromatography) are


    both methods scientists use to analyze samples to determine what the sample contains or the
    concentration of molecules in the sample. Both use the same principle, that heavier molecules will
    elute, or flow, more slowly than lighter ones (polarity also plays a role in elution time). Although the
    idea is the same, GC and HPLC have several differences.

    The Mobile Phase


    o The mobile phase of chromatography equipment is the substance that moves the
    sample through the machine. In HPLC the mobile phase is a liquid made up of an organic solvent,
    ultrapure water and other ingredients to ensure its compatibility with the sample. GC uses gas for its
    mobile phase. Gases used include helium, nitrogen, argon or hydrogen, depending on what is being
    analyzed.

    The Columns
    o As samples travel over chromatography columns, the sample and mobile phase
    interact with the column contents causing the components of the sample to elute at different time.
    HPLC columns are typically four-to-six inch-long metal or glass tubes tightly packed with silica or
    differing carbon chain lengths. GC systems have coiled capillary columns with interior walls coated
    with various materials depending on the lab's needs. Stretched out, GC columns can reach lengths
    of 100 feet.
    The Samples
    o GC is used for volatile compounds (those that break down rapidly) while HPLC is
    better for less volatile samples. If a sample contains salts or carries a charge, it must be analyzed
    using HPLC, not GC.

    Temperature Control
    o GC columns are housed in an oven within the machine. A computer changes the
    temperature while samples are analyzed. The higher the temperature, the faster the sample elutes,
    but temperatures that are too high produce poor results. HPLC columns are kept at a stable
    temperature (most often room temperature) at all times.

    Read more: The Differences Between HPLC & GC | eHow.com http://www.ehow.com/list_7456786_differences-


    between-hplc-gc.html#ixzz1HaJaWyyH

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