PROCHECK

Main-chain parameters
5015679
%-tage of residues in most favoured regions

a. Ramachandran plot quality assessment b. Peptide bond planarity - omega angle sd

100 25

Standard deviation (degrees)

80 20

60 15

40 10

20 5

1.0 1.5 2.0 2.5 3.0 3.5 4.0 1.0 1.5 2.0 2.5 3.0 3.5 4.0
Resolution (Angstroms) Resolution (Angstroms)
c. Measure of bad non-bonded interactions d. Alpha carbon tetrahedral distortion
70 20.0
Zeta angle standard dev. (degrees)
Bad contacts per 100 residues

60 17.5
15.0
50
12.5
40
10.0
30
7.5
20
5.0
10 2.5

1.0 1.5 2.0 2.5 3.0 3.5 4.0 1.0 1.5 2.0 2.5 3.0 3.5 4.0
Resolution (Angstroms) Resolution (Angstroms)
e. Hydrogen bond energies f. Overall G-factor
2.0 1.0
Standard deviation (kcal/mol)

0.5
1.5
0.0
G-factor

-0.5
1.0
-1.0

-1.5
0.5
-2.0

1.0 1.5 2.0 2.5 3.0 3.5 4.0 1.0 1.5 2.0 2.5 3.0 3.5 4.0
Resolution (Angstroms) Resolution (Angstroms)
Plot statistics
Comparison values No. of
No. of Parameter Typical Band band widths
Stereochemical parameter data pts value value width from mean
a. %-tage residues in A, B, L 704 92.3 88.2 10.0 0.4 Inside
b. Omega angle st dev 788 5.7 6.0 3.0 -0.1 Inside
c. Bad contacts / 100 residues 24 3.0 1.0 10.0 0.2 Inside
d. Zeta angle st dev 744 1.1 3.1 1.6 -1.3 BETTER
e. H-bond energy st dev 511 0.7 0.7 0.2 0.2 Inside
f. Overall G-factor 791 0.1 -0.2 0.3 1.1 BETTER

5015679_04.ps