SESSION NUMBER-01

OVERVIEW OF PROCESS
ENGINEERING PRINCIPLES
 COURSE HANDOUT
• Course Objective:

• Course Rationale:

• Course Outcomes:

• Course outcome Indicators:

• Delivery Plan:

• Session Plan:

• Evaluation Plan:
Pre 1940s
Baker’s yeast Solvents Organic acids Amino acids

Probiotics Antibiotics Enzymes

Pre 1980s

biopolymers Vaccine
Industrial Bioprocessing

SCP Biosurfactants
Post 1980s

Biopharmaceuticals rProteins MAb

Plant Bioactive BioDiesel

compounds
 Amino Acids
L-Glutamic acid was the first amino acid to be produced by
microorganisms. Corynebacterium glutamicum

L-Lysine is predominantly produced by Corynebacterium glutamicum

and to some extent by Brevibacterium flavum or B. lactofermentum.

L-Threonine is manufactured industrially by employing either E. coli or

C. glutamicum.

L-phenylalanine can be produced by both E. coli and C. glutamicum.

Aspartic Acid: The aspartase of E. coli is used.

L-Tryptophan: At present, large scale manufacture of tryptophan is

carried out by using the enzyme tryptophan synthase of E. coli.

https://www.biologydiscussion.com/amino-acids/microbial-production-of-7-types-of-
amino-acids/10356
Pringles® BBQ
 Substrate Input and Output
Oxygen Carbon dioxide

Carbon and
Energy Biomass
Sources

Nitrogen
Source CELL Metabolite(s)

Water
Other
requirements
(P, Heat
S,Na,K,Mg,etc…)
• Cells
– Escherichia coli
– Lactic acid bacteria
– Saccharomyces cerevisiae
– Aspergillus niger
– Mammalian cells
Distillation
Optimisation of production of
metabolite
– Analysis of individual processes
• Mass /material input and output
• Energy input and output
• Analysis of chemical reactions
• Calculations
– concentrations(percent weight/percent
volume.normality) of substrate(sugar), reactants
(oxygen, nitrogen) metabolites(alcohol)
– measurement in units of variables (temperature
pressure, volume)
Course Description BTL
Outcomes

CO1 Apply the fundamentals of various units, 3

dimensions and physical
variables related to bioprocess engineering.

CO2 Apply the gas laws in bioprocess industry. 3

CO3 Apply material balances in bioprocess industry. 3

CO4 Apply energy balances in bioprocess industry. 3

 ACTIVE LEARNING-POSTING QUESTIONS

1.What is basic objective of process engineering

principles in biotechnology.