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Atsuete (Bixa Orellana L.) Seeds and Alugbati (Basella Alba L.) Stem Ethanolic Extract As Potential Hair Dye Tested Via Spectrophotometry
Atsuete (Bixa Orellana L.) Seeds and Alugbati (Basella Alba L.) Stem Ethanolic Extract As Potential Hair Dye Tested Via Spectrophotometry
Atsuete (Bixa orellana L.) Seeds and Alugbati (Basella alba L.)
Stem Ethanolic Extract as Potential Hair Dye Tested via
Spectrophotometry
1John V. Bernardo, 2Hazel E. Laureta
1
Pangasinan State University, Binmaley Campus, Pangasinan, Philippines
2
PHINMA – University of Pangasinan, Dagupan City, Pangasinan, Philippines
1
bernardonhojv@gmail.com, 2hazellaureta29@yahoo.com
Abstract
Coloring agents were obtained from natural sources such as plants and animals.
The biosphere is gifted as more than 500 plant species which yield natural dyes. With the
worldwide concern over the use of eco-friendly and biodegradable materials, the use of
natural dyes has undoubtedly once again gained interest and momentum. This study aimed
to analyse the level of absorbance and (%) transmission of Atsuete seeds and Alugbati stem
dye extract through spectrophotometer as potential hair dye substitute synthetic hair dye.
Parallel group design was used in this study with three treatments groups and one control
group and was subjected to spectrophometric analysis at 200nm, 250m, and 400-550nm.
Results showed that Treatment3 is the most potential hair dye coloring agent because it
absorbs less light from ultraviolet to visible light and this may protect the hair strands from
being brittle due to light absorption of color applied in the hair stands. Further,
Treatment3 has the highest transmission among the treatment and control groups with
transmission of 6.028%-6.300% at 200-250nm respectively; and 99.46% at 400-550nm.
High Transmission property of Treatment3 can protect the hair from phototendering due
to light when applied by this dye. Generally, this study found that combination of dyes from
plant can enhance light transmission to help protect hair structure and color from sunlight
exposure and that atsuete and alugbati have a potential as hair dye. Recommendation for
home-made use for the said extract is recommended.
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ISSN 2672-2461 (Print) journal.paressu.org ISSN 2651-247X (Online)
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NATURAL AND SOCIAL SCIENCE
Vol. 4, No. 1, s. 2020
colorants that the effects of radiation, particularly On the other hand, Alugbati (Basella
visible and ultraviolet, can be measured in some alba L.) a plant that thrives in tropical Asia,
quantitative way [Pugh]. Africa, Malaya and is found cultivated
throughout the Philippines. Basella alba fruit
Nowadays, dyes are being subjected to with dark blue skin and deep red violet flesh is a
spectrophotometric analysis. Spectrophotometric potential source of natural colorant. [sk reshmi].
analysis was introduced in 1941 [14] and Different studies have proved that the plant is
spectrophotometry is mainly used for the rich in vitamin A and vitamin C along with
measurement of specific substances and flavonoids, saponins, carotenoids, many amino
aggregate parameters in water and wastewater. acids and organic acids. Various in vivo and in
However, other applications for physical vitro studies revealed that the plants is enriched
parameters like measurement or characterisation with active substances/principles having
of color had also been reported [15]. Some other medicinal potential. Physical and biological
uses of spectrophotometric analysis in research properties of Basella alba stem mucilage has
field are in biostain [16] [17], anti-oxidant [18], studied and reported that mucilage is mainly a
spray dye [19], dye for solar cell [20], and bixa polysaccharide with pH ranging between 5.3 to
dye for food that is tested for absorbance level; 5.4, contains Dgalactose as a major
and optical property test of biosynthesized silver monosaccharide and exhibits slow swelling
nanoparticles [21]. This study aimed to conduct capacity, mild antioxidant activity and no
spectrophotometric analysis on the different ratio tyrosinase inhibiting activity [24]. These two
mixture of Atsuete (Bixa orellana L.) seeds and plants will be utilized for the production and
Alugbati (Basella alba L.) stem ethanolic extract formulation of potential hair dye that can replace
as potential source of hair dye. synthetic hair dye in the market.
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ISSN 2672-2461 (Print) journal.paressu.org ISSN 2651-247X (Online)
P H I L I P P I N E J O U R N A L O F
NATURAL AND SOCIAL SCIENCE
Vol. 4, No. 1, s. 2020
Plate 1. Certificate of
Plant Authentication
Plate 2. One kilogram of Atsuete and
Alugbati and laboratory materials
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Vol. 4, No. 1, s. 2020
c. Spectrophotometric Analysis
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NATURAL AND SOCIAL SCIENCE
Vol. 4, No. 1, s. 2020
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