Dynamics of the Tnt1 retroelement and circadian clock genes in

homeostasis conditions in Nicotiana tabacum

Henrique Moura Dias¹; José Hernandes Lopes Filho²; Juliana Morini Küpper Cardoso Perseguini¹; Marie-Anne Van Sluys²
E-mail: henrique.mouradias@gmail.com; zehernandes@gmail.com; julianam@utfpr.edu.br; mavsluys@usp.br

¹Universidade Tecnológica Federal do Paraná (UTFPR); ²Genomics and Transposable Elements Laboratory (GaTE-Lab) – Universidade de São Paulo (USP)

The demonstration that the activation of
the Tnt1 retroelement is induced by
stress has supported the hypothesis that
under adverse conditions the expression
may be tied to an important mechanism
of gene regulation in pathways related to
plant development, such as the Ethylene
that it may play a role in plant development.
pathway .
Introduction
The regulation that Tnt1 exerts on such
genes occurs only at post-
tress-induced, suggesting transcriptional level (through small
RNAs), or does Tnt1 also be able to
promote transcriptional regulation
through the methylation of promoter
regions of its target genes?

Experimental Procedure Results

1 Biological material 2 Collection of material

12hs 18hs 0h 6hs 12hs 18hs 0h 6hs 12hs

Nicotiana tabacum seedlings with 15 Time (h)

9 collection points in 48 hours
days of in vitro. Germination in
fotoperiod 24h light/dark and
temperature
tress-induced, suggesting
3 Material processing
that it may play a role in plant
development.

RNA isolation

4 RT-qPCR
cDNA synthesis
Gene Category
NtCP-23 Circadian clock
NtTOC1 Circadian clock
ACO1 Ethylene biosynthesis
ER24 Ethylene responsive Conclusion
TEIL Ethylene responsive
These results suggest that although there is a basal expression of the Tnt1 subfamilies, it
JERF1 Ethylene responsive does not follow the circadian rhythm. Induction of Tnt1 expression by ethylene may
U3A Tnt1A qPCR were performed require exposure to higher levels of the phytohormone, and in homeostasis its basal
using Master Mix SYBR
U3B Tnt1B expression may be influenced by other factors that we do not yet understand.
Green
U3C Tnt1C qPCR relative expression
RT Tnt1 all subfamily
Funding Agency: Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP), Coordenação de
Aperfeiçoamento de Pessoal de Nível Superior (CAPES).
5 Data analysis Key-words: Plant development; transposable elements; circadian cycle; molecular genetic.

The expression data were normalized through the reference gene L25, following
data analysis methodology 2-ΔCt (threshold cycle).