18BTC301J-Bioseparation Technology

CT 1
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Among the following, which is not a product of cell disruption method? *

Protein

DNA

Water

RNA

Monoclonal antibodies are belong to ----------------- products *

Sector I

Sector II

Sector III

Sector IV

The objective for High value and Low throughput product are------------- *

Maximum yield and optimal substrate utilization

Maximum purity and optimal substrate utilization

Maximum yield and product concentration

Maximum purity and product concentration

The murein layer thickness for gram positive bacteria is about -------------------
-*

10- 80 nm

20 – 200 nm

1 – 8 nm

1000 nm

Yoghurt obeys a model of ----------------- *

Newtonian

Bingham

Casson

Pseudoplastic

In a empirical model for scale-up of operation, the scale factor (n) for SCP is ----
-------------------------- *

0.4

0.6

0.2

0.7- 0.8
In a RIPP scheme, the fourth P, stands for *

Purity

Purification

Polishing

Packing

The definition, The amount of energy required to reduce the size of the material
is proportional to size reduction ratio follows *

Kirchoff’s law

Rittinger’s law

Fick’s law

Kick’s law

Fluids that require a gradually increasing shear stress to maintain a constant

strain rate are referred to as _______________ *

Rheopectic

Thioxotropic

Pseudoplastic

Dilatant
Which of the following method is the physical method of cell disruption? *

Using solvents

Using French press

Using Osmotic shock

Using detergents

Buffer does not exhibit the following function *

Prevent lipase action

Chelating the metals

Prevent protease action

Prevent cell lysis

The kinetics of bead mill in cell disruption during batch mode of operation is
………………. *

Zero order

First order

Second order

mixed order
Start up expenses in a fermentation industry varies from *

0-5% of the capital cost

15-20% of the capital cost

10-15% of the capital cost

5-10% of the capital cost

Random motion of particle due to intermolecular forces *

Diffussion

Purification

Polishing

Packing

In a 5 step process , if the overall yield is expected to be 50% , then the step yield
is ----------- *

57

67

77

87
The size range of Haemoglobin is --------------- *

Ionic

Macromolecular

Micron

Fine

Osmotic shock of cell disruption removes ------------ *

Perplasmic layer

Cell membrane

Cytoplasmic layer

Peptidoglycan layer

Determine the unknown concentration of protein sample shows OD value of 0.3,

while the corresponding linear regression equation for standard sample is
y=0.325x +0.02 *

0.74 mg/mL

0.8 mg/mL

0.86 mg/mL

0.92 mg/mL
Determine Shear stress of suspension of rod-shaped cell in water with volume
fraction of 0.08, when shear rate is 80 sec-1, the K value for rod shaped cell is 20
*

84 N/M2

10 N/M2

208 N/M2

320 N/M2

Determine shear stress T for a suspension of spherical cell in water with cell
volume fraction of 0.08 when shear rate is 70 s-1 *

66

77

84

90

The original operation cost of a 2 ton volume of brewing is 600 Rs. Determine the
new operation cost of 4 ton volume . *

750.5

909.4

1023.4

1035.6

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