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  • 4a Proteins Data Sheet

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    The document reports on various experiments conducted on proteins such as albumin and gelatin, including how they are precipitated by reagents like heavy metal salts, tannic acid, and picric acid. It also examines the effect of ethanol, strong acids, and ammonium sulfate on proteins and describes several color reaction tests used to identify proteins including the Biuret test, Ninhydrin test, Xanthoproteic test, Millon's test, and Hopkins Cole test. The results of these experiments and tests are listed to characterize the reactions of different proteins.

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    proteins data sheet to use for reference

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    The document reports on various experiments conducted on proteins such as albumin and gelatin, including how they are precipitated by reagents like heavy metal salts, tannic acid, and picric acid. It also examines the effect of ethanol, strong acids, and ammonium sulfate on proteins and describes several color reaction tests used to identify proteins including the Biuret test, Ninhydrin test, Xanthoproteic test, Millon's test, and Hopkins Cole test. The results of these experiments and tests are listed to characterize the reactions of different proteins.

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    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
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    35 views3 pages

    4a Proteins Data Sheet

    Uploaded by

    lucy star
    The document reports on various experiments conducted on proteins such as albumin and gelatin, including how they are precipitated by reagents like heavy metal salts, tannic acid, and picric acid. It also examines the effect of ethanol, strong acids, and ammonium sulfate on proteins and describes several color reaction tests used to identify proteins including the Biuret test, Ninhydrin test, Xanthoproteic test, Millon's test, and Hopkins Cole test. The results of these experiments and tests are listed to characterize the reactions of different proteins.

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    © All Rights Reserved
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    of 3

    PROTEINS

    DATA AND RESULTS:


    I. PRECIPITATION OF PROTEINS

    ALBUMIN GELATIN

    ACTION OF HEAT

    HEAVY METAL SALTS HgCl2

    AgNO3

    PICRIC ACID

    TANNIC ACID

    II. ACTION OF ETHANOL WITH ALBUMIN

    TEST TUBE 1

    TEST TUBE 2

    TEST TUBE 3

    III. ACTION OF STRONG ACIDS

    ALBUMIN GELATIN

    CONCENTRATED H2SO4

    CONCENTRATED HNO3

    CONCENTRATED HCl

    IV. ACTION OF AMMONIUM SULFATE

    3 ml water + ammonium sulfate + 1 ml egg albumin =

    V. BUFFERING ACTION

    TEST TUBE NO. 1


    TEST TUBE NO. 2

    VI. COLOR REACTION TEST

    A. BIURET TEST
    RESULTS

    ALBUMIN

    GLYCINE

    B. NINHYDRIN TEST
    RESULTS

    ALBUMIN

    AGAR

    TYROSINE

    TRYPTOPHAN

    C. XANTHOPROTEIC
    TEST RESULTS

    ALBUMIN

    AGAR

    TYROSINE

    TRYPTOPHAN

    D. MILLON’S TEST
    RESULTS

    ALBUMIN

    AGAR
    TYROSINE

    TRYPTOPHAN

    E. HOPKIN’SCOLE TEST
    RESULTS

    ALBUMIN

    AGAR

    TYROSINE

    TRYPTOPHAN

    Question:

    1.Why are proteins the most important foodstuff?

    2. What is indicated by a positive color reaction with


    a. Biuret’s test? _______________________
    b. Ninhydrin’s test? ___________________
    c. Xanthoproteic’s test? _______________
    d. Millon’s test? _____________________
    e) Hopkins Cole’s test? ________________

    3. What is the basic building blocks of proteins? Draw its basic structure.

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