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Investigating the effects of ethanolic extract of saffron petals (Crocus Satious

L) on apoptosis and alteration of BAX and BCL2 gene expression in MCF_7
breast cancer cell line

Preprint · August 2020

DOI: 10.13140/RG.2.2.29798.04160

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2 authors:

Homeira Khaneshpour Behboud Jafari

Islamic Azad University of Ahar Ahar Branch, Islamic Azad University, Ahar, Iran
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 Investigating the effects of ethanolic extract of saffron petals (Crocus Satious L) on apoptosis
and alteration of BAX and BCL2 gene expression in MCF_7 breast cancer cell line

Homeira Khaneshpour *1,Behboud Jafari1

*1
Department of Microbiology, Ahar Branch, Islamic Azad University, Ahar, Iran

Abstract:
Introduction and Objectives: One of the most common cancers in women is breast cancer, which has
been on the rise in recent years. Therefore, in this study, we evaluated the effects of ethanolic extract of
saffron petals on apoptosis and altered expression of BAX and BCL2 genes in MCF-7 breast cancer cell
line.
Materials and Methods: In this laboratory study, the effect of ethanolic extract of saffron petals on
survival and expression of BAX and BCL2 genes in MCF-7 breast cancer cells were investigated. For
this purpose, MCF-7 breast cancer cells were cultured and treated with 24, 48 and 72 hours by ethanolic
extract of saffron plant with different concentrations. The effect of the extract on cell viability was
assessed by MTT. RNA extraction was performed and cDNA was performed to measure the expression
of BAX and BCL2 genes Real Time PCR.
Results: This study showed that with increasing concentration and time, cell viability decreased
significantly and significantly compared to control samples. Also, the results of Real-Time PCR
showed that the expression of BAX and BCL2 genes at 48 and 72 hours increased and decreased
significantly compared to the control sample.
Conclusion: The results of this study show that ethanolic saffron extract affects the expression of BAX
and BCL2 genes in human breast cancer cells, which is associated with inhibiting the growth of cancer
cells.
Keywords: saffron petals, Breast Cancer, BAX and BCL2 genes
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