Title Rat IL1

Protocol Bioassay Technology

Date/Time 09/07/2021 13:02:50
Technician Juane Plantika Menra, M.Si
Plate ID 01
Unit pg/ml
Reader Setup Endpoint Single 450.0nm Mix off Temp 25.5
Reader Model # xMark
Reader Serial # 10108
Reader Version # 2.02.05
Comments

Using Standard Data Set from Current Experiment.

Linear Fit: Y=slope*X+intercept
20/50/80%: X = 26,668 / 75,547 / 124,427 Y = 0,412 / 1,016 / 1,621
intercept: 0,082 (+/-0,043), slope: 0,012 (+/-0,001)
chi2=0,023, RMS=0,076, r^2=0,992

Standards Report:

Conc Optical
Std # Well
(pg/ml) Density
1 0 A8 0,009
2 10 A7 0,164
3 20 A6 0,348
4 40 A5 0,695
5 80 A4 1,087
6 160 A3 2,024

Data Analysis Report:

Conc SD
Sample ID Well Replicates Mean
(pg/ml) (Conc)
 D9 0,688
K+01 0,688 48,985 0,000
D10 0,688
D11 0,663
K+02 0,667 47,287 0,457
D12 0,671
E1 0,810
K+03 0,800 58,082 1,086
E2 0,791
E3 0,710
K+04 0,770 55,575 6,804
E4 0,829
E5 0,732
K+05 0,761 54,888 3,316
E6 0,790
E7 0,691
K+06 0,634 44,578 6,575
E8 0,576
E9 0,867
K+07 0,873 63,944 0,686
E10 0,879
E11 0,647
K+08 0,589 41,020 6,575
E12 0,532
F1 0,720
K+09 0,698 49,834 2,459
F2 0,677
F3 0,842
K+10 0,842 61,437 0,000
F4 0,842
F5 0,490
K+11 0,528 36,007 4,288
F6 0,565
F7 0,710
K+12 0,700 49,955 1,144
F8 0,690
F9 0,655
K+13 0,658 46,559 0,343
F10 0,661
F11 0,753
K+14 0,736 52,907 1,887
F12 0,720
G1 0,648
K+15 0,655 46,316 0,800
G2 0,662
G3 0,596
K+16 0,597 41,667 0,172
G4 0,599
G5 0,783
K+17 0,764 55,130 2,173
G6 0,745
G7 0,684
K+18 0,648 45,791 4,060
G8 0,613
A9 0,632
K-01 0,649 45,831 1,944
A10 0,666
A11 0,611
K-02 0,597 41,627 1,601
A12 0,583
B1 0,598
K-03 0,585 40,656 1,487
B2 0,572
B3 0,513
K-04 0,574 39,767 6,976
B4 0,635
B5 0,570
K-05 0,570 39,443 0,000
B6 0,570
B7 0,555
K-06 0,517 35,158 4,345
B8 0,479
B9 0,662
K-07 0,628 44,093 3,945
B10 0,593
B11 0,665
K-08 0,670 47,570 0,629
B12 0,676
K-09 C1 0,776 0,776 56,100 0,000
 C2 0,776
C3 0,671
K-10 0,671 47,610 0,000
C4 0,671
C5 0,511
K-11 0,511 34,673 0,000
C6 0,511
C7 0,799
K-12 0,739 53,149 6,804
C8 0,680
C9 0,540
K-13 0,576 39,969 4,174
C10 0,613
C11 0,579
K-14 0,632 44,497 6,118
C12 0,686
D1 0,688
K-15 0,688 48,985 0,000
D2 0,688
D3 0,756
K-16 0,797 57,799 4,688
D4 0,838
D5 0,731
K-17 0,731 52,462 0,000
D6 0,731
D7 0,354
K-18 0,280 15,994 8,462
D8 0,206