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CHE46 3rdlecture StoichiometryofMicrobialGrowthandProductFormation
CHE46 3rdlecture StoichiometryofMicrobialGrowthandProductFormation
Yield coefficients are defined based on the amount of consumption of another material.
A maintenance coefficient is used to describe the specific rate of substrate uptake for cellular
maintenance
Important Quantities and their Definitions
Ideal Chemostat
Important Quantities and their Definitions
Ideal Chemostat
Important Quantities and their Definitions
Summary of Yield Factors for Aerobic Growth of Different
Microorganisms on Various Carbon Sources
Important Quantities and their Definitions
Growth Parameters of Some Organisms Growing
Anaerobically in a Chemostat
Degree of Reduction
Stoichiometric Calculations
In aerobic fermentations, the growth yield per available electron in oxygen molecules is approximately
3.14 ± 0.11 g dw cells/electron when ammonia is used as the nitrogen source. The number of available
electrons per oxygen molecule (O2) is four. When the number of oxygen molecules per mole of substrate
consumed is known, the growth yield coefficient, YX/S, can easily be calculated.
The ATP yield (YX/ATP) in many anaerobic fermentations is approximately 10.5 ± 2 gdw cells/mol ATP.
In aerobic fermentations, this yield varies between 6 and 29. When the energy yield of a metabolic
pathway is known (N moles of ATP produced per gram of substrate consumed), the growth yield YX/S
can be calculated using the following equation:
Example Problem 2
Example Problem 2
References
1. Shuler, M. L., & Kargi, F. (2002). Bioprocess engineering: Basic concepts. Upper Saddle River, NJ:
Prentice Hall.
1. Bailey, J.E. and Ollis, D.F. (1986). Biochemical Engineering Fundamentals (2nd Edition). Mc-Graw
Hill, Inc.
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