Elena Maria O.

Agravante, LPT, PhD

MC 112 Instructor
elenamarie.agravante@ctu.edu.ph

MC 112
Quiz on Some Reactions
(Individual Submission)

Name: Pila, Mary Ella Mae C. Schedule: M&T (3pm – 4:30pm) Date: May 24, 2021
Instruction: Focus only on those reactions discussed in class, and those assigned.
Describe the result when the reagent is added with the specified analyte. You may include to write
the chemical structure of the resulting product (and its characteristics) which is responsible for the
appearance of the end result of the test, if there’s a positive result, and give the name of the test. You may
write “no reaction” if there’s none. (6 points for each number).
Protein (albumin) and CuSO4 solution
When the reagent is added with egg albumin, the egg albumin dispersion would form a bluish violet color,
a positive result and confirming the presence of peptide bonds and therefore is a protein. This test is the
Biuret Test, which is the general test for proteins, that determines the presence of a peptide bond in a
substance.

Methionine and Nitric acid

Nitrous oxide is a commonly used anesthetic that inhibits the activity of methionine synthase, an enzyme
involved in methylation reactions and DNA synthesis and repair.

Therefore, there is no reaction.

Tollen’s reagent and glucose

Glucose, as a carbohydrate, reacts with Tollen's reagent and forms a silver mirror on the inner walls of
the test tube. The formation of a silver mirror on the bottom and sides of the test tube indicates a positive
result. This confirms the presence of reducing sugars. This test is known as the Tollen's Test that identifies
which carbohydrates reacts with Tollens’ reagent and forms a silver mirror on the inner walls of the test
tube.
Tyrosine and Nitric acid
When a hot, concentrated nitric acid reacts with aromatic amino acids such as tyrosine, it forms a yellow-
colored product known as Xantho protein. The appearance of a yellow to orange colored solution indicates
a positive test. This confirms the presence of amino acids. This test is known as the Xanthoproteic Test
that detects the presence of aromatic groups-containing amino acids like tyrosine and tryptophan.

Benedict’s solution and ketohexose

When Benedict's solution and a ketohexose are heated together, the solution changes its color to orange-
red or brick red precipitate. The formation of a reddish precipitate within three minutes indicates a positive
test. This confirms the presence of reducing sugar in the solution. This test is known as the Benedict's
Test that determines the presence or absence of reducing sugar in the solution.

Fructose, a ketohexose, rearranges to form glucose and is oxidized in Benedict's solution.