DNA

Extraction
Activity 3.6

An initial step to do genetic recombination is of course to extract

DNA. In the succeeding exercise/lab activity you are expected
to extract DNA from a selected organism.

MATERIALS:

1 banana Table salt

1 cup of water Ziplock bag
Alcohol Empty plastic
Filter paper glasses
Dishwashing liquid Spoon
 PROCEDURE:

1. Cut the peeled banana

into small pieces and
place it in the zip-lock
bag.

2. Mash the banana by

pressing the bag with
your hands. You could
also use spoon in it.

3. Next we prepare our

solution.
 4. Fill the glass with half
a glass of water.

5. Add 1/2 tablespoon of

salt.

6. Add one tablespoon of

dishwashing liquid. Mix
well.

7. Add the half solution

to the mashed banana.
 8. Make sure to zip the
bag properly. Mix well.

9. You can now filter the

mixture to the filter paper.
Set aside.

10. Add the ice-cold

alcohol to the mixture.

11. Wait for 5 minutes

and observe the mixture.

We can now see the tiny DNA stand present in the mixture.
 CONCLUSION:

The experiment was quite an interesting learning

experience we had. We were bewildered at first as to
how we should perform the task but we researched
about banana extraction and took our time to
understand it. When it was time for us to perform
the experiment, there weren't much confusion about it
but we had to do a second attempt because the first
attempt wasn't a success. The second attempt was
fortunately successful and we were able to extract
the DNA of the banana. In conclusion, this
experiment was very educational for us and it
thought us a lot about DNA extraction. Teamwork
made all the difference as well.

MEMBERS:

Kyla Daneielle Dael

Leslie Benetez
Harel Casipong
Charmi Razonable
John Mell Cabugnason