LEBANESE INTERNATIONAL UNIVERSITY

MEDICAL LAB INTERNSHIP

Training Guidelines
MEDL 499

Fall Semester
Academic Year 2021-2022
Course Instructor: Dr. Alaa Taha

MEDL

Developed by
Dr. Sami EL Khatib
Associate Professor

LEBANESE INTERNATIONAL UNIVERSITY

 Medical Lab Internship Program

Objectives

The objective of internship program is to guide the students in dealing with ambulatory patients referred to
laboratory department for laboratory tests. It provides an overview of careers within the field, education
requirements, and professional organizations. Fundamental information about clinical labs including laboratory
divisions, safety, and quality control is presented. It also deals with technical aspects of all basic laboratory
activities. This program is divided into 2 parts distributed into 2 summer semesters 3 credits each one (360
hours): MEDL 399, MEDL 399.

The first rotation covers laboratory safety and regulations, quality control, basic specimen collection, reagent
preparation and laboratory medical terminology. On the other hand it includes training in hematology, Clinical
Chemistry, parasitology, urine and body fluids analysis.

The second rotation covers different techniques and procedures used in different laboratory analyses including:
Hemostasis, Serology, blood banking, bacteriology, genetics, and special procedures.

The student will be guided to handle and acquire the following duties and regulations:

1. Patients must be treated efficiently and medical confidentiality should be respected.

2. Staff behavior must be of the highest professional level
3. Discussion of results with patient is absolutely prohibited, regardless of results. They should be always
referred to their physician and panic values should be reported to treating physicians and documented
according to each laboratory rules.
4. The student must be presentable especially in reception section as it reflects the image of the laboratory.
5. Eating and drinking are strictly forbidden out of break times.
6. A supervisor of training, elected by the university, is always available to answer students' questions and
polish their technical abilities as well as resolving any conflict and monitor students attendance.

Dr. Sami EL Khatib 1

Medical Lab Internship Program, 3 credits, 360 hours

In this rotation the student should acquire and be familiar with the following laboratory processes:

1. ROTATION-1: laboratory organization and biosafety

2. ROTATION-2: Collection and handling of specimens

3. ROTATION-3: Basic laboratory skills

4. ROTATION-4: Body fluid analysis

5. ROTATION-5: Parasitological testing

6. ROTATION-6: Hematological testing

7. ROTATION-7: Clinical Chemistry and Endocrinology

Dr. Sami EL Khatib 2

 Medical Lab Internship – MEDL

Student’s Information

Student Name

I.D

Cell Phone

Home Phone

Emergency

Graduation Semester

MEDL – Hospital

Current Address

Dr. Sami EL Khatib 3

 Medical Lab Internship – MEDL

Departments Rotations

Department Training period

Specimen reception

Phlebotomy

Hematology

Serology

Blood Bank

Clinical Biochemistry

Microbiology

Parasitology

Urine Analysis

Molecular Diagnosis

Dr. Sami EL Khatib 4

 Medical Lab Internship – MEDL

Phlebotomy – Objectives
1. To disinfect the blood collection site with appropriate disinfectant
2. To know how to apply a tourniquet and for desirable time
3. To detect the preferred venous access sites
4. To insert the needle properly for blood withdrawal
5. To take care of the patient to avoid complications during collection process

Trainer Name

Task Observed Performed

Patient identification

Use of tourniquet

Tubes identification

Blood collection

Syringe & Vacutainers

Detection of venous site

Specimen management

Dr. Sami EL Khatib 5

 Medical Lab Internship – MEDL

Microbiology – Objectives
1. To select appropriate media for various clinical specimens
2. To process specimens for isolation of pathogenic microorganisms
3. To identify microorganisms encountered in the clinical laboratory
4. To exhibit knowledge of environmental influences on microbial growth
5. To differentiate between normal flora and pathogens
6. To interpret antimicrobial sensitivity patterns

Trainer Name

Task Observed Performed

Specimen collection

Media preparation

Specimen inoculation

Gram staining

Colony characteristic

biochemical tests

Dr. Sami EL Khatib 6

 Bacterial identification

Task Observed Performed

Antibiotic susceptibility

Automated systems

Blood culture

Urine culture

Sputum culture

Stool culture

Mycology

Body fluids

Dr. Sami EL Khatib 7

 Medical Lab Internship – MEDL

Parasitology – Objectives
1. To recognize appropriate specimens type and quality for requested tests
2. To preserve and process specimens for requested tests
3. To exhibit knowledge of different types of clinically significant parasites
4. To identify different diagnostic stages of clinically significant parasites
5. To perform urine and semen analysis as part of the duties the laboratory

Trainer Name

Task Observed Performed

Specimen collection

Macroscopic examination

Microscopic examination

Sedimentation test

Staining tests

Biochemical tests

Semen analysis

Dr. Sami EL Khatib 8

 Medical Lab Internship – MEDL

Clinical Biochemistry – Objectives

1. To learn different techniques in clinical chemistry
2. To learn special techniques applied in clinical chemistry
3. To analyze biochemical values for healthy and disease conditions
4. To learn calibration procedures for various tests
5. To apply criteria for calibration acceptance or rejection

Trainer Name

Task Observed Performed

Specimen collection

Reading instructions

Tests principles

Instruments set-up

Operating machines

Samples preparation

Reagents preparation

Dr. Sami EL Khatib 9

 Panic values

Check trouble shootings

Liver function

ALT

AST

AP

Total bilirubin

Direct bilirubin

Indirect bilirubin

Total proteins

Albumin

Globulin A/G ratio

Other tests

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 Renal function

Creatinine

Urea

BUN

Uric acid

Creatinine clearance

Lipid profile

Total cholesterol

Triglycerides

HDL-cholesterol

LDL-cholesterol

VLDL-cholesterol

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 Cardiac markers

Creatinine kinase

LDH

Troponin

Myoglobin

Bone profile

Total calcium

Ionized calcium

Phosphorus

Uric acid

Vitamin D

Parathyroid hormone

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 Pancreatic function

Alpha amylase

Lipase

Special clinical biochemistry

Electrophoresis

Electrophoregram

Hemoglobin

Immunoassays

Tumor markers

Hormones

Dr. Sami EL Khatib 13

 Medical Lab Internship – MEDL

Hematology – Objectives
1. To perform routine hematological tests
2. To prepare and stain blood films with routine and special stains
3. To exhibit knowledge of processing bone marrow specimens
4. To perform or observe special techniques
5. To prepare films from body fluids
6. To apply knowledge of flow cytometry operation and sample preparation

Trainer Name

Task Observed Performed

Specimen reception

Specimen registration

Labelling

Logbook

CBC Bench

Automated counter

Blood film reading

Dr. Sami EL Khatib 14

 Coagulation Bench

Automated tests

PT

PTT

INR

Fibrinogen

Thrombin

D-dimer

Anti-thrombin III

Platelets

Coagulation factors

Dr. Sami EL Khatib 15

 Staining Bench

Blood smear

Leishman stain

Giemsa stain

Malaria parasites

Reticulocyte count

Manual techniques and procedures

ESR

Osmotic fragility test

Sickle cell anemia

Malaria parasites

Body fluids

Bone marrow

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 Medical Lab Internship – MEDL

Blood Banking – Objectives

1. To develop technical accuracy and self-confidence by experiencing routine
functions of Blood Bank
2. To recognize and resolve discrepancies for blood grouping
3. To exhibit knowledge of standard techniques used for ABO and Rh typing,
compatibility testing, antibody identification, antigen typing, and
preparation of blood components
4. To acquaint with the procedures of donor selection and issuing of blood and
blood products for transfusion

Trainer Name

Task Observed Performed

Donor selection

Blood donation

Blood components

Storage

Management of units

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 Pre-transfusion testing

ABO grouping

Rh typing (D, Du)

DAT

Resolve discrepancies

Anti-Human globulin

Cross-matching

Antibody titration

Antibody elution

Dr. Sami EL Khatib 18

 Medical Lab Internship – MEDL

Serology and Immunology – Objectives

1. To acquire knowledge in routine serological and immunological techniques.
2. To use different techniques and equipment to performing routine tests.
3. To exhibit knowledge and importance of blood donors testing, if applicable.

Trainer Name

Task Observed Performed

Agglutination tests

Serial dilution

Titer calculation

ELISA

Immuno-blotting

Western-blot

immunofluorescence

Dr. Sami EL Khatib 19

 Immuno-techniques

RPR

CRP

Widal

Brucella

ASO

HIV

HBV

HTLV

CMV

Nephelometry

Enzyme immunoassay

Quality control

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