Professional Documents
Culture Documents
Herbal Medicine Development
Herbal Medicine Development
Development
Discovery
Target identification
Advantages:
- Pre-selection of potentially active resources
- Promising safety profile (age-long experience)
- Cost-efficient and comparatively fast
Natural product screening
Natural product screening
Process development in herbal medicine
Lead compounds
Identification of lead compounds
Drug targets
Preclinical development
In vitro profiling
Biochemical assays (e.g. enzyme activity
assays)
Cell culture assays (e.g. cancer cell lines)
Isolated tissue assays (e.g. mucosa model)
In vitro toxicology
Investigate potential toxic effects
in bacteria- or cell cultures
Preclinical development
In vivo testing - Animal model (mouse or rat)
Drug action: - Behaviour and reaction
- Physiology
- Histopathology
Toxicology test:
Acute toxicity
Subchronic toxicity
Tissue specific toxicity
Tolerability
Preclinical development
Consider ethical aspects (e.g. number and kind of
animals used).
Objectives:
To develop the pharmacological profile
To determine the acute toxicity in at least 2
animal species
To assess toxicity with studies ranging from
2 weeks to several months
Steps in pre-clinical test
Step One: Get an idea for a drug target.
Drugs usually act on either cellular or genetic chemicals in
the body, known as targets, which are believed to be
associated with disease.
Pharmacological activity
Pharmacokinetics and drug metabolism
Toxicology
Pharmaceutics