SIP180017

SIX1008
BIOCOMPUTING

NAME : PUA WEI YI

NO. MATRIC : SIP180017

ASSIGNMENT : 2

SEMESTER : 1

ACADEMIC SESSION : 2019/2020

COURSE INSTRUCTOR : PROFESSOR MADYA DR. TEOH

TEOH TEOW CHONG

DATE OF SUBMISSION : 9TH OF NOVEMBER, 2019

Page | 1
 SIP180017

Assignment 2 SHES 1603/SIX 1008 2020_1 [25 marks]

Based on the nucleotide sequence below:

1. Write the nucleotide sequence for:

a. T7 promoter [2]
TAATACGACTCACTATAGG

b. lac operator [2]

GGAATTGTGAGCGGATAACAATTCC

c. ribosomal binding site [2]

GAAGGAG

d. 6 x His tag [2]

CACCACCACCACCACCAC

e. NcoI restriction enzyme [2]

CCATGG

f. BamHI restriction enzyme [2]

GGATCC

g. EcoRI restriction enzyme [2]

GAATTC

h. HindIII restriction enzyme [2]

AAGCTT

2. Write the amino acid sequence of the translated protein. [7]

Met K Y L L P T A A A G L L L L A A Q P A Met A Met G R I C R C

I C G R G I C R C I C G V P G V G V P G V G G A T G S D V N F
D L S T A T A K T Y T K F I E D F R A T L P F S H K V Y D I P L
L Y S T I S D S R R F I L L D L T S Y A Y E T I S V A I D V T N
V Y V V A Y R T R D V S Y F F K E S P P E A Y N I L F K G T R
K I T L P Y T G N Y E N L Q T A A H K I R E N I D L G L P A L S
S A I T T L F Y Y N A Q S A P S A L L V L I Q T T A E A A R F
K Y I E R H V A K Y V A T N F K P N L A I I S L E N Q W S A L
S K Q I F L A Q N Q G G K F R N P V D L I K P T G E R F Q V T
N V D S D V V K G N I K L L L N S R A S T A D E N F I T T Met T
L L G E S V V E F P W A L W K T Met L K E L G T Met A L H A G
K A A L G A A A D T I S Q G T Q V P G V G V P G V G K L A A A
L E H H H H H H STOP

Page | 2
 SIP180017

3. Calculate the molecular weight of the protein translated. [2]

The molecular weight of the protein translated is 41282.65 Daltons (using Compute pl/Mw tool)

Page | 3
 SIP180017

>RetroMAD

AGATCTCGATCCCGCGAAATTAATACGACTCACTATAGGGGAATTGTGAGCGGATAACAATTCCCCTC
TAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACAT (5P untranslated regions)

ATGAAATACCTGCTGCCGACCGCTGCTGCTGGTCTGCTGCTCCTCGCTGCCCAGCCGGCGATGGCCAT
GGGGCGTATTTGCCGTTGCATTTGCGGCCGTGGCATTTGCCGCTGCATCTGTGGCGTGCCGGGTGTTG
GTGTTCCGGGTGTGGGTGGTGCGACCGGATCCGATGTGAACTTTGATCTGAGCACCGCGACCGCGAAA
ACCTATACCAAATTCATCGAAGATTTTCGTGCGACCCTGCCGTTTAGCCATAAAGTGTATGATATCCC
GCTGCTGTATAGCACCATTAGCGATAGCCGTCGTTTTATTCTGCTGGATCTGACCAGCTATGCGTATG
AAACCATTAGCGTGGCGATTGATGTGACCAACGTGTATGTGGTGGCGTATCGTACCCGTGATGTGAGC
TACTTTTTCAAAGAAAGCCCGCCGGAAGCGTACAACATTCTGTTTAAAGGCACCCGTAAAATTACCCT
GCCGTATACCGGCAACTATGAAAACCTGCAGACCGCGGCGCATAAAATTCGTGAAAACATCGATCTGG
GCCTGCCGGCCCTGAGCAGCGCGATTACCACCCTGTTTTATTATAACGCGCAGAGCGCGCCGAGCGCG
CTGCTGGTGCTGATTCAGACCACCGCGGAAGCGGCGCGTTTTAAATATATTGAACGCCACGTGGCGAA
ATATGTGGCGACCAACTTTAAACCGAACCTGGCCATTATTAGCCTGGAAAACCAGTGGAGCGCCCTGA
GCAAACAAATTTTTCTGGCCCAGAACCAGGGCGGCAAATTTCGTAATCCGGTGGATCTGATTAAACCG
ACCGGCGAACGTTTTCAGGTGACCAATGTGGATAGCGATGTGGTGAAAGGCAACATTAAACTGCTGCT
GAACAGCCGTGCGAGCACCGCGGATGAAAACTTTATTACCACCATGACCCTGCTGGGCGAAAGCGTGG
TGGAATTCCCGTGGGCGCTGTGGAAAACCATGCTGAAAGAACTGGGCACGATGGCGCTGCATGCGGGT
AAAGCGGCGCTGGGTGCGGCAGCGGATACCATTAGCCAGGGCACCCAGGTTCCGGGCGTGGGCGTTCC
GGGCGTTGGTAAGCTTGCGGCCGCACTCGAGCACCACCACCACCACCACTGA

GATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGC
ATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG (3P untranslated
regions)

Page | 4
 SIP180017

>RetroMAD
AGATCTCGATCCCGCGAAATTAATACGACTCACTATAGGGGAATTGTGAGCGGATAACAATTCCCCTC
TAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGAAATACCTGCTGCCGACCGCTGCTG
CTGGTCTGCTGCTCCTCGCTGCCCAGCCGGCGATGGCCATGGGGCGTATTTGCCGTTGCATTTGCGGC
CGTGGCATTTGCCGCTGCATCTGTGGCGTGCCGGGTGTTGGTGTTCCGGGTGTGGGTGGTGCGACCGG
ATCCGATGTGAACTTTGATCTGAGCACCGCGACCGCGAAAACCTATACCAAATTCATCGAAGATTTTC
GTGCGACCCTGCCGTTTAGCCATAAAGTGTATGATATCCCGCTGCTGTATAGCACCATTAGCGATAGC
CGTCGTTTTATTCTGCTGGATCTGACCAGCTATGCGTATGAAACCATTAGCGTGGCGATTGATGTGAC
CAACGTGTATGTGGTGGCGTATCGTACCCGTGATGTGAGCTACTTTTTCAAAGAAAGCCCGCCGGAAG
CGTACAACATTCTGTTTAAAGGCACCCGTAAAATTACCCTGCCGTATACCGGCAACTATGAAAACCTG
CAGACCGCGGCGCATAAAATTCGTGAAAACATCGATCTGGGCCTGCCGGCCCTGAGCAGCGCGATTAC
CACCCTGTTTTATTATAACGCGCAGAGCGCGCCGAGCGCGCTGCTGGTGCTGATTCAGACCACCGCGG
AAGCGGCGCGTTTTAAATATATTGAACGCCACGTGGCGAAATATGTGGCGACCAACTTTAAACCGAAC
CTGGCCATTATTAGCCTGGAAAACCAGTGGAGCGCCCTGAGCAAACAAATTTTTCTGGCCCAGAACCA
GGGCGGCAAATTTCGTAATCCGGTGGATCTGATTAAACCGACCGGCGAACGTTTTCAGGTGACCAATG
TGGATAGCGATGTGGTGAAAGGCAACATTAAACTGCTGCTGAACAGCCGTGCGAGCACCGCGGATGAA
AACTTTATTACCACCATGACCCTGCTGGGCGAAAGCGTGGTGGAATTCCCGTGGGCGCTGTGGAAAAC
CATGCTGAAAGAACTGGGCACGATGGCGCTGCATGCGGGTAAAGCGGCGCTGGGTGCGGCAGCGGATA
CCATTAGCCAGGGCACCCAGGTTCCGGGCGTGGGCGTTCCGGGCGTTGGTAAGCTTGCGGCCGCACTC
GAGCACCACCACCACCACCACTGAGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGC
TGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG

Deadline: 29/11/2019, 10pm.

Page | 5